Dyes & Cell Imaging Reagents

Product Overview

Product Overview Dyes Cellular Thermoprobe “Cellular Thermoprobe for Fluorescence Lifetime” is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intra- cellular temperature distribution by fluorescence lifetime imaging microscopy. It can be delivered into cell without microinjection. It is highly photostable and easy-to-use. With its cell permeability, it is applicable for both adherent and suspension cells. It enables us to distinguish intracellular temperature in cultured mammalian cells at organelle level. 6 Catalog# Description Quantity Supplier FDV-0004-200 Cellular Thermoprobe for Fluorescence Lifetime 200μg Funakoshi FDV-0004-600 Cellular Thermoprobe for Fluorescence Lifetime 3x200μg Funakoshi Cellular Thermoprobe® for Fluorescence Ratio Cellular Thermoprobe® for Fluorescence Ratio is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence ratio. Cellular Thermoprobe® for Fluorescence Ratio can be delivered into cell without microinjection. In addition, it does not require Fluorescence Lifetime for measuring. That means it is easy-to-use. With its cell permeability, Cellular Thermoprobe® for Fluorescence Ratio is applicable for both adherent and suspension cells. It enables researchers to distinguish intracellular regional temperature at organelles in cultured mammalian cells. Cellular Thermoprobe® for Fluorescence Ratio is an innovative new tool that can provide unprecedented scientific insight Catalog# Description Quantity Supplier FDV-0005-200 Cellular Thermoprobe for Fluorescence Ratio 200μg Funakoshi FDV-0005-600 Cellular Thermoprobe for Fluorescence Ratio 3x200μg Funakoshi Diffusive Thermoprobe Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. Catalog# Description Quantity Supplier FDV-0002 Diffusive Thermoprobe 100μg Funakoshi PELOBiotech GmbH, Am Klopferspitz 19, 82152 Martinsried www.pelobiotech.com • email: info@pelobiotech.com Tel: +49 89 517 286 59 0 • Fax: +49 89 517 286 59 88

Product Overview Dyes Particulate Thermoprobe Particulate Thermoprobe is a fluorescent nanogel thermometer for living cells. It aggregates in proportion to increase in the temperature and fluorescence intensity is proportional to itsaggregation. Fluorescence intensity is observed by fluorescencemicroscopy. Thermoprobe measures the average temperature ofthe whole cells, and it provides unprecedented experimentalapproach. Catalog# Description Quantity Supplier FDV-0003 Particulate Thermoprobe 100μg Funakoshi 7 AcroleinRED - Cell-based Acrolein Detection Reagent Product Background Acrolein, the simplest α, ß-unsaturated aldehyde (chemical name; 2-propenal), is highly toxic metabolite for cells. Acrolein is found in a common dietary and environmental pollutant such as tobacco smoke. Acrolein is also endogenously generated in cells, especially under the oxidative stress condition. Enzymatic oxidation of threonineand polyamine species, reactive oxygen species (ROS)-mediated oxidation of highly unsaturated lipids are majorsource of acrolein in cells. Recent evidences indicate acrolein is more toxic to cells than ROS such as hydrogenperoxide (H2O2) or hydroxyl radicals. Because of its highly reactivity, acrolein in the cell immediately reacts with various biomolecules such as DNA/RNA and proteins, and subsequently impairs their functions. Establishment of the detection methods of cellular acrolein is an important issue to understand physiological relevance of acrolein. AcroleinRED is the world first cell-based acrolein-detection reagent under the live cellcondition without any pre-treatment and cell lysis. This reagent is based on phenylazide-acrolein click chemistry discovered by Drs. Katsunori Tanaka and Ambara Pradipta (Ref.1). AcroleinRED specifically react with either extracellular acrolein released from cell surface lipids or intracellular acrolein generated via enzymatic pathway and label acrolein with TAMRA fluorophore. In the case of extracellular labeling, TAMRA-labeled acrolein are immediately incorporated into cells through the endocytosis pathway. The TAMRA-labeled acrolein react with biomolecules such as a protein and stay in the cells (Figure 1). PELOBiotech GmbH, Am Klopferspitz 19, 82152 Martinsried www.pelobiotech.com • email: info@pelobiotech.com Tel: +49 89 517 286 59 0 • Fax: +49 89 517 286 59 88