ES/iPSs Cells, Media&Tools 2024

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www.pelobiotech.com PB-045-50 HiPSC Freezing Medium: Serum-free 50 ml Cell Applications freezing medium optimized for Human iPSCs PB-cAP-53 iPSC Freezing Medium 50 ml Angio-Proteomie PB-10003-01 and CRYO-Gold (Serum and protein-free) 10 or Revive OrganTech PB-10003-01-20 100 ml PB-10005-01 CRYO-DMSO-Free freezing media 100 ml Revive OrganTech PB-210102 CryoStor CS 10 (Also available is CryoStor CS 2 and 5) Either 10 ml, 100ml, or 1 L bag BioLife Solutions 3.2 Coating Substances or In Vitro Microenvironment for Stem Cells Coating substrates for iPSCs constitute a crucial facet of their culture and differentiation processes, with the choice of coating material exerting a significant influence on cell behavior and fate. Therefore, the selection of coating materials and techniques should be carefully considered to provide an optimal microenvironment for iPSCs to thrive and differentiate effectively. Our extensive network of partners enables us to offer a diverse portfolio of coating solutions for stem cell culture, maintenance, and differentiation. These coating solutions range from undefined sources to precisely chemically defined formulations. The comprehensive suite of solutions listed below reflects our commitment to providing researchers with the tools necessary for creating an optimal environment for the successful culture and differentiation of ES/iPSCs. Some examples include: • iMatrix-511 – xeno-free recombinant laminin 511 E8 (is used with the StemRNA-3 rd Gen Reprogramming Kit) • iMatrix-221 – xeno-free recombinant laminin-211 E8; is used mainly for cardiac and myoblast cells • iMatrix-411 – xeno-free recombinant laminin-211 E8, is mainly used for differentiation into endothelial progenitor cells • ReproCoat – coating for feeder dependent culture of human iPSCs Here are the majority of the products we have available, but there are always more coming from our fantastic partners. If you sign up for our newsletter, you will be the first to hear about the new products. Cat # Product Quantity Supplier 892011 iMatrix-511 2x175μg REPROCELL® 892012 iMatrix-511 (0.5 mg/mL solution) 6x175μg REPROCELL® 892018 Easy iMatrix-511 100 ml REPROCELL® NP892-011 iMatrix 511 175 ug × 2 tube/box REPROCELL® NP892-012 iMatrix 511 (175 ug × 6 tube/box) 1050 μg REPROCELL® NP892-021 iMatrix 511 Silk (175 ug × 1050 µg REPROCELL® 6tube/box) NP892-031 iMatrix 332 (350 ug: 175 ug × 2 tube/box) 350 µg REPROCELL® 10

www.pelobiotech.com NP892-032 iMatrix 332 (1,050 ug: 175 ug × 6 tube/box) 1050 µg REPROCELL® NP892-041 iMatrix 411 (175 ug × 2 tube/box) 350 µg Funakoshi NP892-042 iMatrix 411 (175 ug × 6 tube/box) 1050 µg Funakoshi NP892-043 iMatrix 411 (175 ug × 1 tube/box) 175 µg Funakoshi NP892-061 iMatrix 221 (175 ug × 2 tube/box) 350 µg Funakoshi NP892-062 iMatrix 221 (175 ug × 6 tube/box) 1050 µg Funakoshi NP892-071 iMatrix 111 (350 ug: 175 ug × 2 tube/box) 350 µg REPROCELL® RCHEOT001 ReproCoat 500mL REPROCELL® NP899-001 MatriMix (511) 1 kit (3 vials: 1 Kit REPROCELL® Solution A, B and C) Beside the above listed coating solutions, we offer also organ-derived coating solutions and hydrogel for in vivo-like- 2D/3D culture of cells. The organ derived ECMs and hydrogels are isolated from healthy pigs and can be used for iPS differentiation. Human organ-derived ECMs are also available on request. Cat # Product Quantity Supplier PB-MTSHT101 TissueSpec® Heart ECM Hydrogel 1mL XYLYX Bio PB-MTSIN101 TissueSpec® Intestine ECM Hydrogel 1mL XYLYX Bio PB-MTSKY101 TissueSpec® Kidney ECM Hydrogel 1mL XYLYX Bio PB-MTSLG101 TissueSpec® Lung ECM Hydrogel 1mL XYLYX Bio PB-MTSLV101 TissueSpec® Liver ECM Hydrogel 1mL XYLYX Bio PB-MTSBN101 TissueSpec® Bone 1mL XYLYX Bio PB-MTSSK101 TissueSpec® Skin 1mL XYLYX Bio PhenoDrive from Tissueclick is a ground-breaking class of specialized substrates designed for organotypic tissue cultures and organoids. It is a synthetic biomaterial that facilitates the culture and testing of various cell phenotypes in the form of spheroids and co-culture models, closely mimicking tissue microenvironments like stem cell niches. The biomimetics work seamlessly in 2D/3D culture conditions and adapt to commonly used plasticware. For example, PhenoDrive-I has been shown to promote the differentiation of iPSCs into brain cortical neuron networks with axonal growth in a range of neural cells. Cat # Product Quantity Supplier PB-TC-PD-Y- 0001 PB-TC-PD-I- 0001 PB-TC-PD- U-0001 PB-TC-PD- PS-0001 PhenoDrive Y 1 mg TissueClick PhenoDrive I 1 mg TissueClick PhenoDrive U 1 mg TissueClick PhenoDrive PS 1 mg TissueClick 11