ES/iPSs Cells, Media&Tools 2024

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www.pelobiotech.com Also available are arrays to evaluate the best coating for your application. Two different arrays are available using human biomatrix proteins or GAGs in combination with ECM-peptides. The MicroMatrix Array can be used to test 36 different biomatrix combinations (n=9) on a slide. Cat # Product Quantity Supplier PB-MM012011 MicroMatrix 36 1 pcs MicroStem PB-MM012011-4 MicroMatrix 36-4 Pack 1 pcs MicroStem 3.4 Dissociation Tools for Stem Cells Trypsin-based dissociation can at times be too harsh for iPSCs, so more gentle solutions can lead to better viability. We have the following options: Cat # Product Quantity Supplier PB-076-05 i-HNSC Dissociation Solution 5 ml Cell Applications PB-076-20 i-HNSC Dissociation Solution 20 ml Cell Applications RCHETP002 Dissociation solution for human ES/iPSCs 30 ml REPROCELL® PB-PAACUTASE Cell Passaging Solution (Accutase) 100 ml PELOBiotech 4. Small Molecules, Growth Factors and Cytokines We offer a broad portfolio of small molecules (produced by StemGent of REPROCELL®), growth factors & cytokines (REPROCELL® and Akron Biotech). The growth factors and cytokines are available also as animal component free (ACF) and some are available in cGMP quality. 4.1 Small Molecules Small molecules play a pivotal role in the reprogramming of somatic cells into induced pluripotent stem (iPS) cells, offering versatile tools for overcoming challenges associated with traditional methods. Beyond reprogramming, small molecules have been identified for their ability to modulate the epigenetic state of reprogrammed cells. This means that small molecules do not alter the genome, meaning donor-based genetic factors remain intact during the iPSC derivation process. By mimicking key reprogramming factors, enhancing efficiency, and modulating epigenetic states, small molecules contribute substantially to the refinement and optimization of iPSC generation methods. We carry the following small molecules: Cat # Product Quantity Supplier 04-0014 or 04- Stemolecule A83-01 2 or 10 mg StemGent by REPROCELL® 0014-10 04-0015 Stemolecule ALK5 Inhibitor 1 mg StemGent by REPROCELL® 04-0021 Stemolecule All-Trans Retinoic Acid 100 mg StemGent by REPROCELL® 04-0004 or 04- Stemolecule CHIR99021 2 or 10 mg StemGent by REPROCELL® 0004-10 04-0004-02 Stemolecule CHIR99021 in Solution 2 mg StemGent by REPROCELL® (10mM) 04-0022 Stemolecule Cyclopamine 2 mg StemGent by REPROCELL® 04-0041 Stemolecule DAPT 5 mg StemGent by REPROCELL® 04-0024 Stemolecule Dorsomorphin 2 mg StemGent by REPROCELL® 12

www.pelobiotech.com 4.2 Growth factors and cytokines The differentiation and proliferation of iPSCs are intricately influenced by a spectrum of growth factors and cytokines, showcasing the complexity and versatility of cellular responses. The induction of pluripotent stem cells from fibroblast cultures, as pioneered by Takahashi & Yamanaka (2006), demonstrates how specific factors can impart growth properties and gene expression profiles reminiscent of embryonic stem cells. Thus, they can be differentiated into a multitude of different cell types by using an intricate interplay of growth factors and cytokines in directing the differentiation and proliferation of iPSCs across various cell lineages. Cytokines give stability and flexibility to cells while promoting rapid responses to certain stimuli. We can offer the following growth factors and cytokines: Cat # Product Quantity Supplier 03-0001 Stemfactor Activin A, Human Recombinant 5 μg StemGent by REPROCELL® 03-0007 Stemfactor BMP-4, Human Recombinant 10 μg StemGent by REPROCELL® 03-0002 Stemfactor FGF-basic, Human Recombinant 50 μg StemGent by REPROCELL® 04-0016 Stemolecule Doxycycline hyclate 10 mg StemGent by REPROCELL® SRP002 Stemolecule ec23 5 mg Alvetex by REPROCELL® 04-0025 Stemolecule Forskolin 10 mg StemGent by REPROCELL® 04-0028 Stemolecule KAAD-Cyclopamine 100 μg StemGent by REPROCELL® 04-0074 or 04- Stemolecule LDN-193189 2, 10 mg StemGent by REPROCELL® 0074-02/10 04-0006/-10/- Stemolecule PD0325900 2,10 mg StemGent by REPROCELL® 02 04-0005 Stemolecule Sodium Butyrate 5 mg StemGent by REPROCELL® 04-0017 Stemolecule Thiazovivin 500 mg StemGent by REPROCELL® 04-0007 Stemolecule Valproic Acid 1 mg StemGent by REPROCELL® 04-0034 Stemolecule Wnt Inhibitor IWP-2 5 g StemGent by REPROCELL® 04-0035 Stemolecule Wnt Inhibitor IWP-3 2 mg StemGent by REPROCELL® 04-0036/-50 Stemolecule Wnt Inhibitor IWP-4 2, 50 mg StemGent by REPROCELL® 04-0046 Stemolecule XAV939 50 mg StemGent by REPROCELL® 04-0012 Stemolecule Y27632 2 mg StemGent by REPROCELL® 03-0019- 250 Stemfactor Hepatocyte Growth Factor, Human Recombinant (1 ug/uL) 250 μg StemGent by REPROCELL® 03-0016 Stemfactor LIF, Human Recombinant (10 ug/mL) 1 mL StemGent by REPROCELL® 03-0016- 100 Stemfactor LIF, Human Recombinant (100 ug/mL) 1 mL StemGent by REPROCELL® 03-0011 Stemfactor LIF, Mouse Recombinant (10 ug/mL) 1 mL StemGent by REPROCELL® 03-0011- 100 Stemfactor LIF, Mouse Recombinant (100 ug/mL) 1 mL StemGent by REPROCELL® RCHEOT002 Stemfactor TM human bFGF 25 µg StemGent by REPROCELL® 13