ES/iPSs Cells, Media&Tools 2024

www.pelobiotech.com

www.pelobiotech.com Introduction to our stem cells, media, and tools Classically known for our primary cell portfolio, we also offer the full range of products for pluripotent (Embryonic Stem (ES)) and induced pluripotent stem cell research. Inducible pluripotent stem cells (iPSCs) are pluripotent stem cells artificially derived from adult somatic cells by reprogramming, exhibiting the properties of an embryonic stem cell. In 2006, the team of Professor Shinya Yamanaka at Kyoto University, Japan, was the first to produce iPSCs and now the applications of these cells are endless! Contents Introduction to our stem cells, media, and tools ........................................................................... 4 1. RNA Reprogramming ................................................................................................................ 5 2. Stem Cell Culture Media........................................................................................................... 6 2.1 Feeder-dependent Culture .................................................................................................... 6 2.2 Feeder-free Culture ................................................................................................................ 7 2.3 Differentiation Media for iPSCs ............................................................................................. 7 2.3.1 iPS-Derived Endothelial Cell Differentiation Medium ................................................... 8 2.3.2 Human Cardiomyocyte Differentiation Medium ........................................................... 8 3. Stem Cell Culture Tools ............................................................................................................ 9 3.1 Cryo-Preservation Media for Stem Cells ............................................................................... 9 3.2 Coating Substances or In Vitro Microenvironment for Stem Cells ................................. 10 3.4 Dissociation Tools for Stem Cells ..................................................................................... 12 4. Small Molecules, Growth Factors and Cytokines .................................................................. 12 4.1 Small Molecules ................................................................................................................... 12 4.2 Growth factors and cytokines ............................................................................................. 13 5. Antibodies and Staining Kits .................................................................................................. 14 6. Transfection Reagents ............................................................................................................ 14 7. iPSCs and iPS-Derived Cells .................................................................................................... 15 All images in this brochure have been created with BioRender.com unless stated otherwise. 4

www.pelobiotech.com 1. RNA Reprogramming iPSCs can be obtained through cellular reprogramming, a process which upregulates different combinations of pluripotency-associated genes in somatic cells to revert them back into a pluripotent state. The stem cell field was revolutionized after the partially reprogramming mouse embryonic fibroblasts into iPSCs. This was done via overexpression of the following four mouse transcription factors: Oct4, Sox2, Klf4 and c-Myc using retroviral vectors in 2006. Now different techniques are used for this application ranging from mRNA-based to viral-based reprogramming. Our partner REPROCELL® has developed non-viral reprogramming systems so the resulting iPS-derived cells can be used in clinical applications. Viral reprogramming Non-viral reprogramming Techniques Retro/Lentivirus Adenovirus Sendaivirus Episomal mRNA miRNA+mRNA srRNA nmRNA+miRNA Integrating X Nonintegrating X X X X X X X Clinical grade iPSCs X X X X X Fibroblasts, Fibroblasts, Fibroblasts, Cell Bloodderived EPCs, and urine Blood-derived Fibroblasts Fibroblasts Blood-derived source (s) EPCs EPCs derived cells StemRNA Reprogramming kit The Stem RNA TM 3 rd Gen Reprogramming Kit from REPROCELL® provides the fastest, most efficient method to generate clinically relevant iPSCs using a non-integrating, mRNA-based protocol. Cat # Product Quantity Supplier 00-0076 StemRNA 3 rd Gen 1 Kit StemGent by Reprogramming Kit REPROCELL® This Stem RNA TM 3 rd Gen Reprogramming kit (Cat # 00-0076) has the following features: Cell Type Fibroblasts (adult and neonatal) 5 Blood-derived Endothelial Progenitor Cells (EPCs) Urine-derived Progenitor Cells (UPCs) Non-Modified RNA Reprogramming # of Days to 1° iPSC transfections colonies 6-8 6-8 10-15 days Reprogramming efficiency 2-4% up to 1000 colonies/6-well 0,4-3% up to 200 colonies/6-well 0,1-0,5% up to 20 colonies/6-well 5