ES/iPSs Cells, Media&Tools 2024

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www.pelobiotech.com 2. Stem Cell Culture Media Induced pluripotent stem cells enable researchers to push the boundaries of in vitro models opening opportunities for clinical applications. The designation "iPS" (induced pluripotent stem) is applied to cells that mirror the morphology and growth properties of embryonic stem (ES) cells while expressing essential ES cell marker genes, as elucidated by Takahashi & Yamanaka in 2006. iPSCs can be cultured in various types of media ranging from classic DMEM with specific supplements and serum added to fully chemically defined media. Furthermore, defined media makes the process reliable and without batch effects. We offer media in our own Cellovations® brand and from different suppliers for ES/iPSCs and iPS-derived cells. For iPSCs, feeder cells are often utilized to help maintain their growth while they remain being pluripotent; however, iPSCs can be cultured in either feeder-layer or feeder-free conditions. 2.1 Feeder-dependent Culture Growth factors are needed to foster the growth and proliferation of stem cells. One way of providing these growth factors is through the direct addition of factors such as bFGF into the media. An alternative is with the application of feeder cells which are able to provide growth factors, cytokines, and extracellular matrix components all of which facilitates cell attachment and overall increases cell viability. Interestingly enough, feeder cells have also been shown to preserve the stem cell telomere integrity better ensuring that there is more reliable capabilities of self-renewal (as shown in the Guo et al, 2018 paper published in Nature Communications). For those interesting in using feeder cells for their culture we can provide the following mouse embryonic fibroblasts: Cat # Product Quantity Supplier MUIEF-01002 ICR Mouse Embryonic 1 Vial, or packs of 10 Cyagen Fibroblasts (Irradiated) vials of 0,5x10E6 cells RCHEFC001 SL10: Mouse fibroblast feeder cells applicable for culture of murine, primate, and human PSCs. 5 Vials with 3 Million, Cells/Vial REPROCELL® 6

www.pelobiotech.com 2.2 Feeder-free Culture Feeder-free cell culture media has garnered significant attention due to its versatile applications and inherent advantages in cell culture techniques. Moreover, when the goal is to have a fully xeno-free culture, then the use of the standard feeder cells must be avoided. Moreover, the adoption of a defined, xeno-free, and feeder-free culture system for human iPSCs has proven advantageous, diminishing the lotto-lot variability commonly seen in culture with classical serum and feeder cells. Several products have been developed to facilitate these advancements in feeder-free cell culture. NutriStem XF/FF culture medium for ES/iPSCs stands out as one option for a fully defined, xeno-free medium. The Cellovations Pluripotent Stem Cell Kit for human iPSCs presents another defined and serumfree alternative that does not require bFGF supplementation, and is complete with a specialized coating solution. Another notable product is the Cellovations iPS Growth Medium, which serves as a xeno-free growth medium tailored for ES/IPSCs. These innovations signify a growing commitment to refining and advancing feeder-free culture systems for diverse cell types. We carry the following media for stem cells culturing: Cat # Product Quantity Supplier PB-C-MH-609- Cellovations iPS Growth Medium – 500 ml PELOBiotech 0000-rtu xeno- free and ready-to use RCHEMD001 Primate ES cell medium 500 mL REPROCELL® PB-015-500 HiPSC Growth Medium 500 ml Cell Applications PB-015XF-500 HiPSC Growth Medium Xeno Free 500 ml Cell Applications PB-015XFK-500 HiPSC Growth Medium Kit 500 ml Cell Applications PB-015K-500 Human Induced Pluripotent Stem Cell Yields 500 Cell Applications (HiPSC) Growth Medium Kit ml HUXES-90011 Human Embryonic Stem Cell Growth Kit Cyagen Medium MUXES-90011 Mouse Embryonic Stem Cell Growth Kit Cyagen Medium MUXES-90051 EB formation Medium Kit Cyagen PB-cAP-49 Human iPSC SFM Culture Medium, 500 ml Angio-Proteomie Xeno- Free 01-0005-100 NutriStem hPSC XF Medium 100 ml or 500 ml available REPROCELL® Check out our GMP products in our portfolio. We offer growth factors, cytokines, media supplements (e.g. A & AB-serum, human platelet lysate (hPL), HAS), and cryopreservation media. 2.3 Differentiation Media for iPSCs iPSCs can be differentiated into any type of cell through the application of specific supplemented small molecules in the media. Differentiation media for induced pluripotent stem (iPS) cells play a pivotal role in steering the fate of these cells toward specific lineages. A plethora of studies have delved into the exploration of distinct culture media to effectively promote the differentiation of iPSCs into targeted cell types. 7