ES/iPSs Cells, Media&Tools 2024

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www.pelobiotech.com 2.3.1 iPS-Derived Endothelial Cell Differentiation Medium For reference, here is the description of one of those such media. iPS-Derived Endothelial Cells Differentiated Media is designed for the differentiation of iPS into endothelial cells. The medium contains growth factors and supplements such as: • Ham's F-12 Nutrient Mix • IMDM • N2-SUPPLEMENT • B-27 Supplement Minus • 10% BSA • MTG (1-Thioglycerol,0.45M,1000x) • Ascorbic acid Before use, one must always add the specific growth factors needed into 100ml of the supplemented medium. These can include 0.1ml VEGF, 20µl FGF-2, and 4µl BMP-4. Cat # Product Quantity Supplier PB-M6861 IPS-Derived Endothelial Cell Differentiated Medium Kit Cell Biologics 2.3.2 Human Cardiomyocyte Differentiation Medium We have one set media kit for the differentiation of iPSCs into cardiomyocytes. You must note however, that this is for non-commercial research use only (RUO) and thus is unable to be used for any clinical applications. Cat # Product Quantity Supplier 017DK Human Cardiomyocyte Differentiation Medium Kit Kit Cell Applications 8

www.pelobiotech.com More differentiation media will be coming soon! For example, stay tuned for media for hematopoietic progenitors and hepatocytes. 3. Stem Cell Culture Tools Beside stem cell media we additionally offer tools for cryo-preservation, coating, dissociation and transfection. Moreover, we have set tools for 3D culturing! 3.1 Cryo-Preservation Media for Stem Cells Cryopreservation stands as a crucial technique for the long-term storage of induced pluripotent stem (iPS) cells, safeguarding their viability and functionality upon thawing. As a matter of fact, extensive work has delved into the optimization of cryopreservation media, particularly for various types of stem cells. We carry a wide range of cryopreservation media options that come in GMP and RUO quality. Moreover, we have some with and without DMSO, and many remain xeno-free. REPROCELL®'s freezing medium, formulated by stem cell expert Prof. Nakatsuji and colleagues at Kyoto University, Japan, for human stem cells, ensures high levels of viability, surpassing typical viability obtained with conventional 10% DMSO solutions. These advancements in the options of what you can purchase signify ongoing efforts to enhance the efficacy and applicability of cryopreservation techniques in the field of stem cell research and therapy. DMSO-containing Cryo-Media • CryoStor CS10 o Embryonic Stem Cells & iPSCs, Mesenchymal Stem Cells (MSCs), Adult Stem Cells (available in GMP) • CRYO-GOLD TM o Embryonic Stem Cells & iPSCs, Mesenchymal Stem Cells, Adult Stem Cells, Primary Cells, and Cell lines • NutriFrez TM (REPROCELL®) o Embryonic Stem Cells & iPSCs, Mesenchymal Stem Cells (MSCs), • Stem Cell Freezing Medium for ES, iPS & adult Stem Cells (defined; protein- & serum-free) DMSO-free Cryo-Media (Defined and Xeno-free) • CryoScarless o Embryonic Stem Cells & iPSCs, Mesenchymal Stem Cells • CRYO-DMSO-F o Embryonic Stem Cells & iPSCs, Mesenchymal Stem Cells Cryo-Medium for Vitrification (DMSO-free, Defined & Xeno-free) • StemCell Keep o Embryonic Stem Cells & iPSCs For your reference, here are the item numbers for the cryo-preservation solutions we have: Cat # Product Quantity Supplier 01-0020-50 NutriFreez D10 Cryopreservation 50 ml REPROCELL® Medium (50mL) CPL-A1 CryoScarless DMSO-Free (Serum & 100 ml Funakoshi DMSO-free) VPL-A1 StemCell Keep 20 ml Funakoshi 9