PeloNews Fall 2019

PELONews New Products

PELONews New Products New Kits on the Block: Endothelial Transwell Permeability Assay Kit Our partner Cell Biologics has developed a simple, reproducible, sensitive, quantitative transwell permeability assay to measure endothelial barrier function for wide analyses of multiple types of endothelial permeability in vitro. The assay will be performed by a spectrometer-based absorbance reader (ELISA plate reader) at 450 nm within one hour post endothelial treatment. The assay kit is suitable for primary endothelial cells and immortal cell lines from multiple species. The microvascular endothelial cell monolayer localized at the critical interface between the blood and vessel wall has the vital functions of regulating tissue fluid balance and supplying the essential nutrients needed for the survival of the organism. The endothelial cells are able to dynamically regulate its paracellular and transcellular pathways for transport of plasma proteins, solutes, and liquid. MORE Please click here: https:// www.yumpu.com/s/ oAlv6Dx375zsgkiE The endothelial barrier is a well-regulated structure, which maintains a minimal and selective permeability to fluid and molecules under normal physiological conditions. Vascular leakage is an important feature in several diseases, such as septic shock, inflammation, disruption of blood-brain barrier (BBB)-related neurological disorders, viral hemorrhagic fever, cancer and ischemiareperfusion injuries. It is emergent to develop a highly sensitive and reliable assay to measure endothelial permeability. ATAC-seq Kit The ATAC-seq kit is a complete set of optimized reagents that are ideal to generate indexed DNA libraries for ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing). The Kit includes Tn5 Transposase and tagmentation reagents, and also library construction reagents (index-primers, enzyme et al), with this kit you can obtain qualified ATAC-Seq library DNA ready to perform sequencing on Illumina sequencer. Feature Low input, requires sample as low as 50,000 live cells Fast workflow, with minimal hands-on time MORE Please click here: https:// www.yumpu.com/s/ Pr5iWyCENMgI39bq Robust, reliable performance Ultra high-fidelity amplification with minimized GC bias Application: Prepare ATAC-seq DNA library for Illumina sequencer

World's First Medium for Mouse (HSCs) Hematopoietic Stem Cells for Maintaining Undifferentiated State HemEx-TypeA is a serum-free media, which contains Polyvinyl Alcohol (PVA) and can be used for mouse Hematopoietic Stem Cells (HSCs). Features Optimized for the expansion of mouse HSCs, Hematopoietic Stem and Progenitor Cell (HSPCs) obtained from bone marrow. Can do expansion culture of HSCs, for more than 1 month in undifferentiated state. PVA is used instead of albumin. In general, culture media for HSCs contains serum albumin. Such albumin is required to induce cell division of HSCs. On theother hand, albumin inhibits stable undifferentiated state. Widely used purified or recombinant albumin contains small amount of contaminants,but are tough to remove. It is reported that such contaminants induce differentiation of HSCs. Moreover, protein's oxidation reaction inducescellular senescence. Thefore, albumin needs to be replaced by other chemicals. Dr. Satoshi Yamazaki at The Institute of Medical Science, The University of Tokyo reported that PVA can replace albumin (1). Moreover, PVA is a stable chemical with no oxidation reaction in medium so aging of HSCs are also suppressed. These advantages help to do expansion culture of HSCs while maintaining undifferentiated state. Protocol Please see the paper of Nature, 571, 117 - 121 (2019). Note : For culture, addition of cytokine is required. Reference: (1) Adam C., et al. Nature, 571, 117 - 121 (2019). Long-term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation (paper is attached).