PeloNews Fall 2019

PELONews IPS/ES Special

PELONews IPS/ES Special Features Easy to use Works in various cells - especially good for iPS / ES cells Reduce the risk of contamination by xenocomponents in your culture system. High cell viability and proliferation rate. Ready to use laminin-511 fragment: Easy iMatrix-511 series No more laborious calculation for dilution of coating matrix! iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells or Transgenic silkworm cocoon. They can reduce the risk of the xeno-components contamination in the culture. MORE Please click here: https:// www.yumpu.com/s/ yLV7o3vPlnZj6N8U ) Promotes greater stem cell adhesion than all other matrix proteins that have been tested E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511 Background: Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511". Features Specific for undifferentiated human iPS or ES cells. Uniformly stain cell membrane of human iPS or ES cells. Can be used for removing undifferentiated human iPS or ES cells by cytotoxic effect. Clone : R-17F Please click here MORE https://www.pelobiotech.com/wpcontent/uploads/2018/08/ IPSelector.png iPS/ES cells Specific Monoclonal Antibody : iPSelector iPSelector is a mouse monoclonal antibody recognizes for lacto-N-fucopentaose I (LNFP I) raised against human iPS cells. LNFP I is the sugar chain expressed in undifferentiate state human iPS or ES cells then this antibody can be used as a marker for undiffrentiated human iPS or ES cells. Moreover, this antibody has a function for cytotoxic effect by binding to undifferentiated human iPS or ES cells. Therefore this antibody is beneficial for removing undifferentiated human iPS or ES cells. Reference 1.Kawabe, K., et al., Glycobiology, 23, 322, (2013). 2. Matsumoto, S., et al., J. Biol. Chem., 290, 20071, (2015). Nakao, H., et al., Glycoconj. J., DOI 10.1007/s10719-0169-9710-2 Pic: Western blot (Funakoshi) One major positive band and several minor bands were specific to human iPS cells, and any positive band were not obtained with HEK293 cells.

New Webinar Take it personal! New Customized Media for Animal & Human cell culture You have your very specific cell culture needs in your lab. Because you are unique and your research is so too.In this webinar with CSO Dr. Lothar Steeb, PELOBiotech, you´ll get an overview of our services to customize your academic or industrial research and production needs. Biomedical research is increasingly focusing on genetic profiles, marker invention and other patterns that characterise cells and processes. More and more, gene analytical investigations are moving into the focus of experiments. Exosomes, miRNAs, smallRNAs and epigenetics play an increasingly important role. We contribute to this development by manufacturing special media which are coordinated with the customer on a trial and cell basis. We offer the customer every modification of classical media as well as new chemically and thus serum-free media variations, which he needs in order to exclude disturbing factors from the outset and thus avoid false positive or false negative results. Examples are RPMIs, DMEMs, SILAC BIT, chemically defined renal epithelial cell media or all types of glucose-free media. Sign up and click here: https://attendee.gotowebinar.com/ register/2810266360323725836 or https://www.pelobiotech.com/peloacademy/ NEW Ways: Grow Cells Quicker with UltraGro UltraGRO-PURE meets your need for a consistent and reliable human protein based cell culture supplement that can support your cell production from research through to clinical trial use. UltraGRO-PURE contains abundant growth factors and cytokines necessary for cell growth and proliferation to replace fetal bovine serum (FBS) for growing Adipose-Derived (AD), Bone Marrow (BM) or Umbilical Cord (UC) Mesenchymal stem cells (MSCs). Growth kinetics for MSCs expanded in 5% UltraGRO-PURE containing medium are comparable to cultures grown in medium supplemented with 20% FBS. UltraGRO-PURE grows cells quicker and safer, lowering your costs per volume of cells grown. More infos here: https://www.yumpu.com/s/cvIPngFTbGiR0lK6 Ask Peter, Lothar and Muriel for your Personal Sample: mailto: info@pelobiotech.com PELOAcademy Don´t have time on these lIVE events? No worries. Sign up and we send you the recording asap. You are very welcome. Want to stay tuned in and informed? Then sign up for our regular Webinar Invitation Reminder. Just check the box with the infos you like to receive and sign up here. Sign up here Learn more October 10, 2019 10 AM (CET/MEZ) Please forward this invitation to your co-workers and friends. Thank you. Features -> Cost-effective, nonxenogeneic / serum substitute ->Natural source and natural signal for cell growth -> Abundant growth factors / cytokines / proteins that maximize MSC growth -> Lot to lot consistency Extended storage for without losing potency -> Research grade and GMP grade available -> Cost-effective, nonxenogeneic / serum substitute