PeloNews Januar 2019

PELONews New Products

PELONews New Products New Mitochondria Uncoupling Agent Ppc-1 Increasing mitochondria's oxigen consumption without inhibiting ATP synthesis Ppc-1, MitoUncoupler Increasing mitochondria's oxigen consumption without inhibiting ATP synthesis Ppc-1, MitoUncoupler a newly isolated molecule from Polysphondylium pseudo-candidum. It shows comparatively mild uncoupling activity in Mitochodria. According to papers, following effects are reported in Ppc-1. Ppc-1 stimulates oxygen consumption without inhibiting ATP synthesis in mitochondria, isolated 1), 2) from mouse liver. Ppc-1 administrated mouse shows inhibition of weight gain, without affecting liver and kidney. Cell proliferation is inhibited without inducing cell death, in Ppc-1 treated 3T3-L1 cells. 1), 2) Features Comparatively mild uncoupling activity. Low effect on ATP synthesis or degradation. Low active oxygen generation. Low cytotoxicity. Cell proliferation is inhibited by adding Ppc-1 to culture medium. Chemical Name :3-Methylbut-2-enyl 4-methoxy-8-(3-methylbut-2-enyloxy)quinoline-2- carboxylate Molecular Formula :C 21 H 25 NO 4 Molecular Weight :355.43 Purity :98% Soluble in DMSO Reference Learn more 1) Suzuki T, et. al, PLoS One. 2015 Feb10; 10(2):e0117088. 2) Kikuchi H, et. al, Bioorg Med Chem., 23(1): 66-72(2015). 3) Kikuchi H, et. al, Tetrahedron, 66: 6000-6007(2010). Learn more

Newly developed Immunosuppressor PQA-18 PQA-18 is a newly developed immunosuppressor. PQA-18 strongly inhibits p21-activated kinase 2 (PAK2), which is related to IL-2, IL-4, IL-6 and TNF-alpha production. PQA-18 is the abbreviation of "Prenylated Quinolinecarboxylic Acid compound 18", and following effects are reported. PAK2 activity is reduced in a dose-dependent manner, when PQA-18 is added to Jurkat cells. IL-2, IL-4, IL-6 and TNF-alpha production are reduced, when PQA-18 is added to human peripheral lymphocytes stimulated for 24 hours by ConA (10 ug/ml). Antigen specific immune reactions is inhibited when PQA-18 is intraperitoneally administered to mouse. Nc/Nga mouse (13 weeks old), applied petrolatum ointment (100 mg) containing PQA-18 shows less skin irritation and blood IgE amount is reduced. Higher stability in blood compared to FK-506. Compatible with in vivo and in vitro application. Molecular Formula : C 25 H 31 NO 4 Features Molecular Weight (Average) : 409.52 Purity : >98% Solubility : DMSO If you see insoluble, try vortex or heat at 37C. Recommended stock solution concentration is 10 - 20 mM. Example Data PQA-18 is added to Jurkat cells. IL-2 production is reduced in a dose-dependent manner. Learn more Reference Ogura M, et al, Biochem Pharmacol, 105:55-65 (2016).