PeloNews with Focus on Liver/Islets

PELONews

PELONews Liver/Pancreas 2D/3D models Go natural: Take native Hydrogels Xylyx Bio has developed the native proteins and growth factors from the respective organ tissue for the cell culture and thus reproduce a more natural environment. The ECM is available as coating, hydrogel or scaffold (custom order) for liver, pancreas-and much, much more. Pancreas: 2D Surface Coating , 3D Scaffold Liver: 2D Surface Coating , 3D Hydrogel & Scaffold Take your pick: Pancreas ECM cell culture substrates Exxtracellular matrix (ECM) is a major component of the cell microenvironment ong>withong> important signaling and regulatory functions. Derived from healthy acellular porcine porcine tissue, NativeCoat and TissueSpec® pancreas matrix is rich in collagen types I, III, IV but also includes collagen type V. TissueSpec® liver matrix is the organizational framework consisting of fibronectin and collagen types I, III, IV, V, and VI that mediates hepatic endothelial and epithelial cell communication, modulates liver homeostasis, and supports repair and regeneration. Excellent Growth Results for Dog Primary Liver Organoids The very innovative company Noviocell has developed Noviogel. It is a synthetic matrix, free of animal and undefined components. The hydrogel for cell culture behaves similar to collagen or fibrin: Your Plus: - functionalized ong>withong> RGD peptides or Pure - thermosensitive and reversible But look for yourself! Already cultured successfully: Dog primary liver organoids ong>withong> Noviogel-RGD: Dog primary liver cells were cultured in Matrigel and noviogel-RGD for 7 days. Liver organoids cultured in noviogel-RGD showed excellent growth characteristics, more organoids and bigger in size. 3D Co Culture Model for Liver, Pancreas Tumor Cells & more Have a look at theese Applicable Cell Systems for Pancreas and Liver Tumor cells. These cells have been tested sucessfuly for aggregate sphere formation by our academic partners. Full overview here: https://biopply.com/assets/Uploads/Applicable-Cell- Systems.PNG Details are described in the publication: A deep conical agarose microwell array for adhesion independent threedimensional cell culture and dynamic volume measurement Andreas R. Thomsen et al., Lab Chip, DOI 10.1039/

Revolution of Enzyme Requirements for Human Islet Isolation by Bob McCarthy The quality of collagenase used for mammalian islet isolation has been a focus of clinical research scientists soon after Ballinger and Lacy demonstrated that islet transplantation could correct chemically induced diabetes in inbred strains of rats (1). Clostridium histolyticum collagenase became a critical reagent because of the need to isolate sufficient numbers of functional islets for islet transplantation in higher mammals (e.g., dogs, pigs, humans). Typically, researchers evaluated several “lots” of crude or enriched commercial collagenase products (i.e., traditional collagenase) for their ability to recover pancreatic islets. The goal of lot qualification was to identify a “good” lot of traditional collagenase that worked successfully in the islet isolation procedure before purchasing larger amounts of a specific lot of product for routine use. Good lots for human islet isolation were hard to find. Camillo Ricordi’s development of a semi-automated method for human islet isolation led him to focus on screening new collagenase lots once these lots were released for sale by a supplier. During this process, Ricordi screened lots of Collagenase P, a crude collagenase product first sold by Boehringer Mannheim Biochemicals in 1990. He found that this material worked better for this application than competitive collagenase products. One lot, lot 9, was used to isolate islets for many allo-islet transplantation procedures (2). Collagenase P had higher collagenase and lower protease activity than found in Sigma collagenase products used by many research scientists at that time. Ricordi’s use of the Collagenase P product in clinical trials led to a meeting ong>withong> R&D management at Boehringer Mannheim Diagnostics in Indianapolis, Indiana about Boehringer solving the “collagenase problem.” This discussion led to funding of a research project at Boehringer Mannheim Biochemicals to identify and purify the key enzymes in crude collagenase responsible for releasing islets from porcine or human pancreatic tissue. The assumption was that the identification, purification, and formulation of purified enzymes used at an appropriate dose would resolve the collagenase problem. To read full paper, please visit: https://www.vitacyte.com/news/evolution-enzymerequirements-human-islet-isolation-link-recent-review/ Reference: 1 Ballinger WF, Lacy PE. Transplantation of intact pancreatic islets in rats. Surgery. 1972;72(2):175-86. 2 Ricordi C, Tzakis AG, Carroll PB, Zeng YJ, Rilo HL, Alejandro R, et al. Human islet isolation and allotransplantation in 22 consecutive cases. Transplantation. 1992;53(2):407-14. Enzymes VitaCyte’s defined enriched (DE) collagenase products set a new standard of consistency and enables assessment of a broader range of collagenase: protease ratios for cell isolation than found ong>withong> current collagenase products. DE Collagenase is designed ong>withong> increasing amounts of enriched collagenase added to a fix amount of purified BP protease. Overview Rodent Islet Overview discussion of common protocols used in rodent islet isolations. Includes a link to a white paper that summarizes a survey of the affect of rodent strain, age, sex, or nutritional state on expected islet yields when purified enzymes were used to isolate islets. https:// www.vitacyte.com/ product-applications/ rodent-islet-applications