ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
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(Phase Collapse)<br />
.( 2 ﻞﮑﺷ)<br />
ﺪﻨﮐ ﯽﻣ ﺍﺪﯿﭘ ﺭﺍﺪﯾﺎﭘﺎﻧ<br />
" ﻦﮐﺎﺳ ﺯﺎﻓ ﺵﺰﯾﺭوﺮﻓ<br />
" ﻡﺎﻨﺑ یﺍ ﻩﺪﯾﺪﭘ ﻉﻮﻗو<br />
ﺖﻟﺎﺣ ﻥﻮﺘﺳ و ﻪﺘﻓﺎﯾ<br />
ﺶﻫﺎﮐ " ﺍﺪﯾﺪﺷ<br />
Column: Zorbax SB-C18, 4.6 x 150 mm<br />
Mobile Phase: 10% Acetonitrile<br />
90% 0.05 M Phosphate Buffer<br />
Sample: 1. Uracil<br />
2. Nitroethane<br />
3. Phetalic acid<br />
4. 4-chloroaniline<br />
5. 3-cyanobenzoic acid<br />
6. 3,5-dimethylaniline<br />
7. 1-nitrobutane<br />
وﺮﻓ " ﺪﻨﯾﺍﺮﻓ ،ﯽﻟﻮﻤﻌﻣ<br />
C18 ﺎﯾ C8 یﺎﻫ ﻥﻮﺘﺳ ﺭﺩ ﯽﺑﺁ " ﺍﺪﯾﺪﺷ کﺮﺤﺘﻣ ﺯﺎﻓ ﻥﺪﻧﺎﻣ<br />
ﻝﻮﻃ ﺕﺪﻣ ﺭﺩ C18 ﻥﻮﺘﺳ ﻞﺧﺍﺩ ﺭﺩ ﻞﮑﺷ ﻦﯾﺍ ﺭﺩ . ﺪﺸﺨﺑ ﯽﻣ ﺕﺪﺷ ﺍﺭ " ﻦﮐﺎﺳ ﺯﺎﻓ ﺵﺰﯾﺭ<br />
ﺎﺑ ﻪﺑﺎﺸﻣ ﻼﻣﺎﮐ ﻂﯾﺍﺮﺷ ﺖﺤﺗ B ﻡﺍﺮﮔﻮﺗﺎﻣوﺮﮐ ﺪﻌﺑ ﺯوﺭ و ﻩﺪﺷ ﻪﺘﺷﺍﺩ ﻪﮕﻧ ﺏﺁ ،ﺐﺷ<br />
ﻥﺎﻣﺯ ﺪﯾﺪﺷ ﺶﻫﺎﮐ ﻩﺪﻨﻫﺩ ﻥﺎﺸﻧ B ﻡﺍﺮﮔﻮﺗﺎﻣوﺮﮐ . ﺖﺳﺍ ﻩﺪﺷ<br />
ﺪﺷﺎﺑ ﯽﻣ " ﻦﮐﺎﺳ ﺯﺎﻓ ﺵﺰﯾﺭ وﺮﻓ<br />
( 2)<br />
ﻞﮑﺷ<br />
ﻪﺘﻓﺮﮔ A ﻡﺍﺮﮔﻮﺗﺎﻣوﺮﮐ<br />
" ﻩﺪﯾﺪﭘ ﻉﻮﻗو ﻞﯿﻟﺩ ﻪﺑ یﺭﺍﺩﺯﺎﺑ<br />
ﺭﻮﻀﺣ ﺭﺩ ،C8<br />
ﺎﯾ C18 ﻞﺜﻣ ﮏﯿﺑﻮﻓوﺭﺪﯿﻫ ﻦﮐﺎﺳ ﺯﺎﻓ یﺯﺎﻓ ﺵﺰﯾﺭوﺮﻓ<br />
ﺎﯾ C18 ﯽﺋﺎﻀﻓ ﺭﺎﺘﺧﺎﺳ ﯽﮔﺩﺭﻮﺧ ﻢﻬﺑ ﯽﻨﻌﻣ ﻪﺑ ﯽﺑﺁ " ﺍﺪﯾﺪﺷ کﺮﺤﺘﻣ ﺯﺎﻓ<br />
ﺭﺩ .( 3 ﻞﮑﺷ)<br />
ﺪﺷﺎﺑ ﯽﻣ ﻦﮐﺎﺳ ﺯﺎﻓ ﺭﺩ ﺎﮑﯿﻠﯿﺳ ﻪﯾﺎﭘ<br />
ﻪﺑ ﻩﺪﺷ ﻩﺩﺍﺩ ﺪﻧﻮﯿﭘ<br />
C8<br />
ﺝﺭﺎﺧ ﻪﺑ ﺎﮑﯿﻠﯿﺳ ﺢﻄﺳ ﺯﺍ ﻢﻈﻨﻣ ﻞﮑﺸﺑ C8 ﺎﯾ C18 ﻩوﺮﮔ ﯽﻟﻮﻤﻌﻣ ﺖﻟﺎﺣ<br />
ﺎﺑ ﺶﻨﮐﺍو یﺍﺮﺑ ﯽﺒﺳﺎﻨﻣ ﯽﻓﺍﺮﮔﻮﺗﺎﻣوﺮﮐ ﺢﻄﺳ ﻦﯾﺍﺮﺑﺎﻨﺑ و ﺪﻧﺍ ﻪﺘﻓﺎﯾ ﺩﺍﺪﺘﻣﺍ<br />
یﺯﺎﻓ ﺵﺰﯾﺭوﺮﻓ ﺯﺍ ﺲﭘ ﻪﮐ ﯽﻟﺎﺣ ﺭﺩ ،ﺪﻨﻨﮐ<br />
ﯽﻣ ﺩﺎﺠﯾﺍ کﺮﺤﺘﻣ ﺯﺎﻓ و ﻪﻧﻮﻤﻧ<br />
ﻪﻧﻮﻤﻧ ﺍﺬﻟ و ﻩﺪﺷ ﻢﮐ " ﺍﺪﯾﺪﺷ ﺢﻄﺳ ﻦﯾﺍ ﻥﺍﺰﯿﻣ و ﻩﺩﺭﻮﺧ ﻢﻬﺑ ﻢﻈﻨﻣ ﻞﮑﺷ ﻦﯾﺍ<br />
یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﻪﺠﯿﺘﻧ ﺭﺩ و ﺩﻮﺷ ﻪﺘﺷﺍﺩ ﺯﺎﺑ ﻥﻮﺘﺳ ﺭﺩ ﺩﺎﯾﺯ ﺭﺍﺪﻘﻣ ﻪﺑ ﺪﻧﺍﻮﺗ ﯽﻤﻧ<br />
،ﻪﯿﻟوﺍ<br />
ﻖﯾﺭﺰﺗ ﺭﺩ . ﺖﺳﺍ ﻕﺎﻔﺗﺍ ﻦﯾﺍ ﺯﺍ ﯽﻌﻗﺍو ﯽﻟﺎﺜﻣ ( 4)<br />
ﻞﮑﺷ . ﺪﺑﺎﯾ ﯽﻣ ﺶﻫﺎﮐ<br />
ﺎﺑ ﺎﻣﺍ ﺪﻧﺍ ﻩﺪﺷ ﺍﺪﺟ ﻢﻫ ﺯﺍ ﻼﻣﺎﮐ و ﻩﺩﻮﺑ ﺐﺳﺎﻨﻣ یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ یﺍﺭﺍﺩ ﺎﻬﮑﯿﭘ<br />
ﻞﺧﺍﺩ ﺭﺩ ﻪﻘﯿﻗﺩ 10 ﻂﻘﻓ ﺕﺪﻣ ﻪﺑ ﯽﺑﺁ ﺪﺻﺭﺩ 100 کﺮﺤﺘﻣ ﺯﺎﻓ ﻦﺘﺷﺍﺩ<br />
ﻪﮕﻧ<br />
ﺎﻬ<br />
ﻠﺣ ﻩﺍﺭ و ﯽﺑﺁ " ﺍﺪﯾﺪﺷ کﺮﺤﺘﻣ ﺯﺎﻓ ﺎﺑ ﺭﺎﮐ ﺕﻼﮑﺸﻣ<br />
20<br />
ﺭﺩ ﯽﺑﺁ کﺮﺤﺘﻣ ﺯﺎﻓ ﺎﺑ یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﯽﻓﺎﮐ یﺮﯾﺬﭘ ﺭﺍﺮﮑﺗ ﻡﺪﻋ<br />
ﻪﻧﻮﻤﻧ یﺯﺎﺳﺍﺪﺟ ﻡﺎﮕﻨﻫ<br />
ﻥوﺭﺍو ﺯﺎﻓ یﺎﻬﻧﻮﺘﺳ<br />
،ﻥوﺭﺍو ﺯﺎﻓ ﯽﻓﺍﺮﮔﻮﺗﺎﻣوﺮﮐ ﺭﺩ ﻪﮐ ﺖﺳﺍ ﻝﻮﻤﻌﻣ<br />
ﺖﻤﺴﻗ ﻦﯾﺮﺘﺸﯿﺑ ﻪﮐ ﯽﮐﺮﺤﺘﻣ ﺯﺎﻓ ﺯﺍ ،ﺏﺁ ﺭﺩ ﺮﯾﺬﭘ ﻞﺣ ﯽﺒﻄﻗ ﺭﺎﯿﺴﺑ یﺎﻫ<br />
( ﻞﯾﺮﺘﯿﻧﻮﺘﺳﺍ و ﻝﻮﻧﺎﺘﻣ ﻞﺜﻣ)<br />
ﻥﺁ ﯽﻟﺁ ﻝﻼﺣ و ﻩﺩﻮﺑ ﯽﺑﺁ ﻝﻼﺣ<br />
ﮏﯾ<br />
یﺯﺎﺳﺍﺪﺟ و ﯽﻓﺎﮐ یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﺎﺗ ﺩﻮﺷ ﻩﺩﺎﻔﺘﺳﺍ ﺖﺳﺍ ﺪﺻﺭﺩ 10 ﺯﺍ ﺮﺘﻤﮐ<br />
ﻥﺎﻣﺯ یﺮﯾﺬﭘ ﺭﺍﺮﮑﺗ ﻡﺪﻋ ﻪﺑ ﻻﻮﻤﻌﻣ ﻂﯾﺍﺮﺷ ﻦﯾﺍ ﺎﻣﺍ . ﺪﯾﺁ ﺖﺳﺪﺑ ﯽﺒﺳﺎﻨﻣ<br />
ﻦﯾﺍ ﻪﮐ ،ﻩﺪﺷ ﺮﺘﻫﺎﺗﻮﮐ ﺭوﺮﻣ ﻪﺑ یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﯽﻨﻌﯾ . ﺩﻮﺷ ﯽﻣﺮﺠﻨﻣ یﺭﺍﺩﺯﺎﺑ<br />
(Resolution) یﺯﺎﺳﺍﺪﺟ ﺖﯿﻔﯿﮐ ﺶﻫﺎﮐ ﻪﺑ ﺮﺠﻨﻣ ﺖﯾﺎﻬﻧ ﺭﺩ ﺮﻣﺍ<br />
.( 1 ﻞﮑﺷ)<br />
ﺩﻮﺷ ﯽﻣ<br />
Column: YMC ODS<br />
Mobile Phase: 5% CH3OH/ 95% 0.1 M KH2PO4<br />
Flow Rate: 1 mL/min<br />
Sample: 1. Vitamin C 2. Vitamin B6<br />
3. Vitamin B1 4. Ni<strong>co</strong>tinamide<br />
ﻞﯿﻟﺩ ﻪﺑ Ni<strong>co</strong>tinamide یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﺭﺎﮐ ﺖﻋﺎﺳ 72 ﺯﺍ ﺲﭘ<br />
. ﺪﺑﺎﯾ ﯽﻣ ﺶﻫﺎﮐ ﺪﺻﺭﺩ 8 ﺯﺍ ﺶﯿﺑ " ﻦﮐﺎﺳ ﺯﺎﻓ ﺵﺰﯾﺭوﺮﻓ"<br />
( 1)<br />
ﻞﮑﺷ<br />
ﺭﺩ (no flow) ﻥﺎﯾﺮﺟ ﻥوﺪﺑ ﯽﺑﺁ " ﺍﺪﯾﺪﺷ کﺮﺤﺘﻣ ﺯﺎﻓ ﻪﭽﻧﺎﻨﭼ ﻝﺎﺣ<br />
ﻞﯿﻟﺩ ﻪﺑ<br />
ﺎﻬﮑﯿﭘ یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ یﺪﻌﺑ یﺎﻫﺰﯿﻟﺎﻧﺁ ﺭﺩ ،ﺪﺷﺎﺑ<br />
ﻩﺪﻧﺎﻣ ﯽﻗﺎﺑ ﻥﻮﺘﺳ<br />
ﻥﺁ
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