ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.
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یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﻪﮐﺪﻧﻮﺷ ﯽﻣ ﺖﻓﺎﯾ ﻥوﺭﺍو ﺯﺎﻓ<br />
HPLC<br />
ﺭﺩ یﺩﺭﺍﻮﻣ ﻪﺘﺒﻟﺍ<br />
ﻪﮐ ﺖﺳﺍ ﯽﻌﻗﺍﻮﻣ ﻪﺑﺮﺼﺤﻨﻣ ﺩﺭﺍﻮﻣ ﻦﯾﺍ . ﺪﻨﮐ ﯽﻣ ﺮﯿﯿﻐﺗﺮﻓﺎﺑ ﺖﻈﻠﻏ ﺎﺑ ﻪﻧﻮﻤﻧ<br />
یوﺭ یﺪﯿﺳﺍ یﺎﻫ ﻝﻮﻧﻼﯿﺳو یﺯﺎﺑ یﺎﻫ ﻪﻧﻮﻤﻧ ﻦﯿﺑ ﯽﻧﻮﯾ ﺶﻨﮐ ﻢﻫﺮﺑ ﺎﻬﻧﺁﺭﺩ<br />
ﻡﺎﮕﻨﻫ ﻕﺎﻔﺗﺍ ﻦﯾﺍ ﺎﻣﻮﻤﻋ . ﺪﻫﺩ ﯽﻣ ﺥﺭ ﻦﮐﺎﺳﺯﺎﻓ ﻪﯾﺎﭘ یﺎﮑﯿﻠﯿﺳ ﺢﻄﺳ<br />
ﯽﻨﮐﺎﺳﺯﺎﻓ ﺯﺍ ﻩﺪﺷﺮﭘ ﻥوﺭﺍوﺯﺎﻓ یﺎﻫ ﻥﻮﺘﺳ ﺭﺩ یﺯﺎﺑ یﺎﻫ ﻪﻧﻮﻤﻧ یﺯﺎﺳﺍﺪﺟ<br />
ﻩﺩﺎﻔﺘﺳﺍ ﻡﺎﮕﻨﻫ ﺮﺘﺸﯿﺑ و ﺖﺳﺍ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﻝﺎﻌﻓ یﺎﻫ ﻝﻮﻧﻼﯿﺳ یﺍﺭﺍﺩ ﻪﮐ<br />
،ﺩﺭﺍﻮﻣ ﻪﻧﻮﮔ ﻦﯾﺍ ﺭﺩ . ﺪﻫﺩ ﯽﻣ یوﺭ 3 ﺯﺍ ﺮﺘﮔﺭﺰﺑ pH ﺎﺑ کﺮﺤﺘﻣ یﺎﻫﺯﺎﻓﺯﺍ<br />
ﻥﺁ ﯽﻧﻮﯾ ﺕﺭﺪﻗ<br />
ﺶﯾﺍﺰﻓﺍ ﻪﺠﯿﺘﻧ ﺭﺩو کﺮﺤﺘﻣﺯﺎﻓ ﺮﻓﺎﺑ ﺖﻈﻠﻏ ﺶﯾﺍﺰﻓﺍ<br />
یﺭﺍﺩﺯﺎﺑﺮﺛﺍ ،ﻪﺠﯿﺘﻧ ﺭﺩو ﻩﺩﺮﮐ ﻢﮐ ﺍﺭ ﺭﻮﮐﺬﻣ ﯽﻧﻮﯾ ﺶﻨﮐ ﻢﻫﺮﺑ ﺪﻧﺍﻮﺗ ﯽﻣ<br />
ﺪﻫﺩ ﺶﻫﺎﮐ ﺍﺭ (Se<strong>co</strong>ndary retention effect) ﻪﯾﻮﻧﺎﺛ<br />
ﻥوﺭﺍوﺯﺎﻓ HPLC ﺭﺩ ﻝﻮﻤﻌﻣ یﺎﻫﺮﻓﺎﺑ ﯽﺧﺮﺑ – 1 ﻝوﺪﺟ<br />
: یﺮﻓﺎﺑ کﺮﺤﺘﻣﺯﺎﻓ ﻪﯿﻬﺗ<br />
: ﺪﻧﻮﺷ ﯽﻣ ﻪﯿﺻﻮﺗﺮﯾﺯ یﺎﻫ ﻡﺎﮔ یﺮﻓﺎﺑ کﺮﺤﺘﻣﺯﺎﻓ ﻪﯿﻬﺗ یﺍﺮﺑ<br />
ﻝوﺍﺪﺟ ﻪﺑﺮﺘﻬﺑ یﺮﯿﮔ ﻢﯿﻤﺼﺗ یﺍﺮﺑ . ﺪﯿﻨﮐ ﺏﺎﺨﺘﻧﺍ ﺐﺳﺎﻨﻣﺮﻓﺎﺑ<br />
ﮏﯾ<br />
. ﺪﯿﻨﮐ ﻉﻮﺟﺭ 2 و 1<br />
ﺩﺭﻮﻣ<br />
pH<br />
و ﺖﻈﻠﻏﺎﺑ<br />
(<br />
ﺏﺁﺭﺩ ﻩﺪﺷ ﻞﺣﺮﻓﺎﺑ)<br />
ﯽﺑﺁﺮﻓﺎﺑ ﮏﯾ<br />
. ﺪﯿﻨﮐ ﻪﯿﻬﺗﺮﻈﻧ<br />
-1<br />
-2<br />
3<br />
: ﺢﯿﺤﺻﺮﻓﺎﺑ ﺏﺎﺨﺘﻧﺍ<br />
ﻪﮐﺪﻨﻨﮐ ﯽﻣ ﻩﺩﺎﻔﺘﺳﺍ ﯽﯾﺎﻬﺷوﺭ ﺯﺍ ﯽﻓﺍﺮﮔﻮﺗﺎﻣوﺮﮐ ﻥﺍﺮﺑﺭﺎﮐ ﺕﺎﻗوﺍﺮﺜﮐﺍ<br />
یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﺎﯾ ،ﻩﺩﺎﻔﺘﺳﺍ<br />
ﺩﺭﻮﻣ pH ﺭﺩ ،ﺎﻬﻧﺁﺭﺩ ﻩﺩﺎﻔﺘﺳﺍ ﺩﺭﻮﻣ یﺎﻫﺮﻓﺎﺑ<br />
ﻪﺗﺎﻔﺳﺎﺘﻣ . ﺖﺳﺍ ﻢﮐﺭﺎﯿﺴﺑ ﻥﺁ ﺭﺍﺪﻘﻣ ﺎﯾو ﺪﻧﺭﺍﺪﻧ (Buffer Capacity)<br />
، pH ﻩﺯﺎﺑﺭﺩ ﺕﺎﺘﺳﺍﺮﻓﺎﺑ ﺎﯾو 6 ﺎﺗ 4 ،pH<br />
ﻩﺯﺎﺑﺭﺩ ﺕﺎﻔﺴﻓﺮﻓﺎﺑ ﻪﮐ ﯽﯾﺎﻬﺷوﺭ<br />
ﻪﻧ ، pH ﻩﺩوﺪﺤﻣ ﻦﯾﺍﺭﺩﺎﻫﺮﻓﺎﺑ ﻦﯾﺍ . ﺪﻨﺘﺴﯿﻧ ﻢﮐ ﺪﻨﻨﮐ ﯽﻣ ﻪﯿﺻﻮﺗﺍﺭ 7 و 6<br />
ﺮﺗ ﺖﺨﺳ ﺍﺭ کﺮﺤﺘﻣﺯﺎﻓ یﺯﺎﺳ ﻩﺩﺎﻣﺁ ﺕﺭوﺮﺿ ﻥوﺪﺑ ﻪﮑﻠﺑ ﺪﻧﺮﺛﺍ ﯽﺑ ﺎﻬﻨﺗ<br />
ﻪﺑ ﺍﺭ ﺮﯾﺬﭘﺭﺍﺮﮑﺗ ﺕﺭﻮﺻ ﻪﺑ یﺯﺎﺳﺍﺪﺟ ﻝﺮﺘﻨﮐ ﻂﻠﻏﺮﮑﻓ و ﺪﻨﻨﮐ ﯽﻣ<br />
. ﺪﻨﯾﺎﻤﻧ ﯽﻣ ﺎﻘﻟﺍﺮﺑﺭﺎﮐ<br />
ﻪﺑ<br />
. ﺪﺘﻓﺍ ﯽﻣ ﻕﺎﻔﺗﺍﺮﻓﺎﺑ<br />
pKa<br />
ﺎﺑ ﻝﺩﺎﻌﻣ<br />
pHﺭﺩ<br />
یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﻦﯾﺮﺘﻬﺑ<br />
ﻪﮐ ﺩﻮﺑ ﺪﻨﻫﺍﻮﺧ ﻝﻮﺒﻗ ﻞﺑﺎﻗ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ یﺍﺭﺍﺩ ﯽﻣﺎﮕﻨﻫ ﺎﻫﺮﻓﺎﺑ ﯽﻠﮐﺭﻮﻃ<br />
ﻥﺁ pKa ﺭﺍﺪﻘﻣ ﺯﺍ ﻑﻼﺘﺧﺍ ﺪﺣﺍو ± 1ﺮﺜﮐﺍﺪﺣ<br />
ﺎﺑ یﺍ ﻩﺩوﺪﺤﻣﺭﺩ ﺎﻫ ﻥﺁ pH<br />
. ﺩﻮﺑﺪﻫﺍﻮﺨﻧ ﻝﻮﺒﻗ ﻞﺑﺎﻗ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﺕﺭﻮﺼﻨﯾﺍﺮﯿﻏﺭﺩ ،ﺪﺷﺎﺑ ﻪﺘﺷﺍﺩ ﺭﺍﺮﻗ ﺎﻫ<br />
. ﺪﻫﺩ ﯽﻣ ﻥﺎﺸﻧ ﺍﺭ ﻥوﺭﺍوﺯﺎﻓ<br />
HPLC ﺭﺩ ﻝوﺍﺪﺘﻣ یﺎﻫﺮﻓﺎﺑﺯﺍ ﯽﻀﻌﺑ 1 ﻝوﺪﺟ<br />
UV cut off و pH ﻝﺮﺘﻨﮐ یﺍﺮﺑ ﻪﻨﯿﻬﺑ ﻩﺯﺎﺑ ، pKa ﺭﺍﺪﻘﻣ ﻝوﺪﺟ ﻦﯾﺍﺭﺩ<br />
. ﺖﺳﺍ ﻩﺪﺷ ﻩﺩﺭوﺁﺰﯿﻧ ﺎﻫﺮﻓﺎﺑ<br />
ﺯﺎﺳﺭﺎﮑﺷﺁ ﻪﺑ ﻪﮐﺪﻧﻮﺷ ﯽﻣ ﺖﻓﺎﯾ ﯽﯾﺎﻫ HPLC ﻻﻮﻤﻌﻣ ﻪﮑﯿﯾﺎﺠﻧﺁﺯﺍ<br />
ﺰﯿﻧ LC/MS یﺎﻫﺩﺮﺑﺭﺎﮐ یﺍﺮﺑ ﺭﺍﺮﻓ یﺎﻫﺮﻓﺎﺑ ،ﺪﻨﺷﺎﺑ ﻞﺼﺘﻣ MASS<br />
. ﺪﻧﺍ ﻩﺪﺷ ﺺﺨﺸﻣ<br />
ﯽﻤﮐﺮﯿﺛﺎﺗ ،ﻥوﺭﺍوﺯﺎﻓ<br />
HPLC<br />
: ﺮﻓﺎﺑ ﺖﻈﻠﻏ<br />
ﺭﺩ کﺮﺤﺘﻣﺯﺎﻓ یﺎﻫﺮﻓﺎﺑ ﺖﻈﻠﻏﻻﻮﻤﻌﻣ<br />
یﺍﺮﺑ . ﺪﻨﮐ ﻝﺮﺘﻨﮐ ﺍﺭ pH ﻪﮐﺪﺷﺎﺑ یﺪﺣﺭﺩ ﺪﯾﺎﺑ ﻂﻘﻓ و ﺩﺭﺍﺩ یﺭﺍﺩﺯﺎﺑﺭﺩ<br />
ﺖﯾﺎﻔﮐﺭﻻﻮﻣ ﯽﻠﯿﻣ 50 ﺎﺗ 25 ﻦﯿﺑ ﺮﻓﺎﺑ<br />
ﺖﻈﻠﻏ ،ﻥوﺯﺍوﺯﺎﻓ یﺎﻫﺩﺮﺑﺭﺎﮐﺮﺜﮐﺍ<br />
ﺭﺩ ﺏﻮﺳﺭ ﺩﺎﺠﯾﺍ ﺯﺍ ﻪﮐ ﺖﺳﺍ ﻢﮐ ﻥﺎﻨﭼ ﺖﻈﻠﻏ ﻦﯾﺍ ﻝﺎﺣ ﻦﯿﻋ ﺭﺩ . ﺪﻨﮐ ﯽﻣ<br />
ﯽﻟﺁ یﺎﻫ ﻩﺪﻨﻨﮐ ﺡﻼﺻﺍ یﺍ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﺭﺍﺪﻘﻣ ﻪﮑﯿﻣﺎﮕﻨﻫ ،ﻢﺘﺴﯿﺳ<br />
یﺮﯿﮔﻮﻠﺟ ،ﺪﻧﻮﺷ ﯽﻣ ﻩﺩﺎﻔﺘﺳﺍ کﺮﺤﺘﻣﺯﺎﻓﺭﺩ (Organic Modifiers)<br />
ﺮﺛﺍ ﻥﺪﻧﺎﺳﺭ ﻞﻗﺍﺪﺣ ﻪﺑ یﺍﺮﺑ ﻕﻮﻓ ﺖﻈﻠﻏ ﺰﯿﻧ ﺕﺎﻔﺴﻓ ﺮﻓﺎﺑ ﺩﺭﻮﻣﺭﺩ . ﺩﻮﺷ<br />
ﺮﺘﻤﮐ ﺖﻈﻠﻏ ﺎﺑﺮﻓﺎﺑﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ . ﺖﺳﺍ ﺐﺳﺎﻨﻣ ﭗﻤﭘ یﺎﻫ<br />
seal یوﺭ ﯽﺒﯾﺮﺨﺗ<br />
.<br />
ﺖﺳﺍ ﻩﺪﺷ ﺵﺭﺎﻔﺳ ﺕﺭﺪﻧ ﻪﺑ ﺭﻻﻮﻣ ﯽﻠﯿﻣ 10ﺯﺍ
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