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5 years ago

ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.

ﭘﯿﮏ ﻫﺎی ﺩﻧﺒﺎﻟﻪ ﺩﺍﺭ - Behan chemical co.

یﺭﺍﺩﺯﺎﺑ

یﺭﺍﺩﺯﺎﺑ ﻥﺎﻣﺯ ﻪﮐﺪﻧﻮﺷ ﯽﻣ ﺖﻓﺎﯾ ﻥوﺭﺍو ﺯﺎﻓ HPLC ﺭﺩ یﺩﺭﺍﻮﻣ ﻪﺘﺒﻟﺍ ﻪﮐ ﺖﺳﺍ ﯽﻌﻗﺍﻮﻣ ﻪﺑﺮﺼﺤﻨﻣ ﺩﺭﺍﻮﻣ ﻦﯾﺍ . ﺪﻨﮐ ﯽﻣ ﺮﯿﯿﻐﺗﺮﻓﺎﺑ ﺖﻈﻠﻏ ﺎﺑ ﻪﻧﻮﻤﻧ یوﺭ یﺪﯿﺳﺍ یﺎﻫ ﻝﻮﻧﻼﯿﺳو یﺯﺎﺑ یﺎﻫ ﻪﻧﻮﻤﻧ ﻦﯿﺑ ﯽﻧﻮﯾ ﺶﻨﮐ ﻢﻫﺮﺑ ﺎﻬﻧﺁﺭﺩ ﻡﺎﮕﻨﻫ ﻕﺎﻔﺗﺍ ﻦﯾﺍ ﺎﻣﻮﻤﻋ . ﺪﻫﺩ ﯽﻣ ﺥﺭ ﻦﮐﺎﺳﺯﺎﻓ ﻪﯾﺎﭘ یﺎﮑﯿﻠﯿﺳ ﺢﻄﺳ ﯽﻨﮐﺎﺳﺯﺎﻓ ﺯﺍ ﻩﺪﺷﺮﭘ ﻥوﺭﺍوﺯﺎﻓ یﺎﻫ ﻥﻮﺘﺳ ﺭﺩ یﺯﺎﺑ یﺎﻫ ﻪﻧﻮﻤﻧ یﺯﺎﺳﺍﺪﺟ ﻩﺩﺎﻔﺘﺳﺍ ﻡﺎﮕﻨﻫ ﺮﺘﺸﯿﺑ و ﺖﺳﺍ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﻝﺎﻌﻓ یﺎﻫ ﻝﻮﻧﻼﯿﺳ یﺍﺭﺍﺩ ﻪﮐ ،ﺩﺭﺍﻮﻣ ﻪﻧﻮﮔ ﻦﯾﺍ ﺭﺩ . ﺪﻫﺩ ﯽﻣ یوﺭ 3 ﺯﺍ ﺮﺘﮔﺭﺰﺑ pH ﺎﺑ کﺮﺤﺘﻣ یﺎﻫﺯﺎﻓﺯﺍ ﻥﺁ ﯽﻧﻮﯾ ﺕﺭﺪﻗ ﺶﯾﺍﺰﻓﺍ ﻪﺠﯿﺘﻧ ﺭﺩو کﺮﺤﺘﻣﺯﺎﻓ ﺮﻓﺎﺑ ﺖﻈﻠﻏ ﺶﯾﺍﺰﻓﺍ یﺭﺍﺩﺯﺎﺑﺮﺛﺍ ،ﻪﺠﯿﺘﻧ ﺭﺩو ﻩﺩﺮﮐ ﻢﮐ ﺍﺭ ﺭﻮﮐﺬﻣ ﯽﻧﻮﯾ ﺶﻨﮐ ﻢﻫﺮﺑ ﺪﻧﺍﻮﺗ ﯽﻣ ﺪﻫﺩ ﺶﻫﺎﮐ ﺍﺭ (Secondary retention effect) ﻪﯾﻮﻧﺎﺛ ﻥوﺭﺍوﺯﺎﻓ HPLC ﺭﺩ ﻝﻮﻤﻌﻣ یﺎﻫﺮﻓﺎﺑ ﯽﺧﺮﺑ – 1 ﻝوﺪﺟ : یﺮﻓﺎﺑ کﺮﺤﺘﻣﺯﺎﻓ ﻪﯿﻬﺗ : ﺪﻧﻮﺷ ﯽﻣ ﻪﯿﺻﻮﺗﺮﯾﺯ یﺎﻫ ﻡﺎﮔ یﺮﻓﺎﺑ کﺮﺤﺘﻣﺯﺎﻓ ﻪﯿﻬﺗ یﺍﺮﺑ ﻝوﺍﺪﺟ ﻪﺑﺮﺘﻬﺑ یﺮﯿﮔ ﻢﯿﻤﺼﺗ یﺍﺮﺑ . ﺪﯿﻨﮐ ﺏﺎﺨﺘﻧﺍ ﺐﺳﺎﻨﻣﺮﻓﺎﺑ ﮏﯾ . ﺪﯿﻨﮐ ﻉﻮﺟﺭ 2 و 1 ﺩﺭﻮﻣ pH و ﺖﻈﻠﻏﺎﺑ ( ﺏﺁﺭﺩ ﻩﺪﺷ ﻞﺣﺮﻓﺎﺑ) ﯽﺑﺁﺮﻓﺎﺑ ﮏﯾ . ﺪﯿﻨﮐ ﻪﯿﻬﺗﺮﻈﻧ -1 -2 3 : ﺢﯿﺤﺻﺮﻓﺎﺑ ﺏﺎﺨﺘﻧﺍ ﻪﮐﺪﻨﻨﮐ ﯽﻣ ﻩﺩﺎﻔﺘﺳﺍ ﯽﯾﺎﻬﺷوﺭ ﺯﺍ ﯽﻓﺍﺮﮔﻮﺗﺎﻣوﺮﮐ ﻥﺍﺮﺑﺭﺎﮐ ﺕﺎﻗوﺍﺮﺜﮐﺍ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﺎﯾ ،ﻩﺩﺎﻔﺘﺳﺍ ﺩﺭﻮﻣ pH ﺭﺩ ،ﺎﻬﻧﺁﺭﺩ ﻩﺩﺎﻔﺘﺳﺍ ﺩﺭﻮﻣ یﺎﻫﺮﻓﺎﺑ ﻪﺗﺎﻔﺳﺎﺘﻣ . ﺖﺳﺍ ﻢﮐﺭﺎﯿﺴﺑ ﻥﺁ ﺭﺍﺪﻘﻣ ﺎﯾو ﺪﻧﺭﺍﺪﻧ (Buffer Capacity) ، pH ﻩﺯﺎﺑﺭﺩ ﺕﺎﺘﺳﺍﺮﻓﺎﺑ ﺎﯾو 6 ﺎﺗ 4 ،pH ﻩﺯﺎﺑﺭﺩ ﺕﺎﻔﺴﻓﺮﻓﺎﺑ ﻪﮐ ﯽﯾﺎﻬﺷوﺭ ﻪﻧ ، pH ﻩﺩوﺪﺤﻣ ﻦﯾﺍﺭﺩﺎﻫﺮﻓﺎﺑ ﻦﯾﺍ . ﺪﻨﺘﺴﯿﻧ ﻢﮐ ﺪﻨﻨﮐ ﯽﻣ ﻪﯿﺻﻮﺗﺍﺭ 7 و 6 ﺮﺗ ﺖﺨﺳ ﺍﺭ کﺮﺤﺘﻣﺯﺎﻓ یﺯﺎﺳ ﻩﺩﺎﻣﺁ ﺕﺭوﺮﺿ ﻥوﺪﺑ ﻪﮑﻠﺑ ﺪﻧﺮﺛﺍ ﯽﺑ ﺎﻬﻨﺗ ﻪﺑ ﺍﺭ ﺮﯾﺬﭘﺭﺍﺮﮑﺗ ﺕﺭﻮﺻ ﻪﺑ یﺯﺎﺳﺍﺪﺟ ﻝﺮﺘﻨﮐ ﻂﻠﻏﺮﮑﻓ و ﺪﻨﻨﮐ ﯽﻣ . ﺪﻨﯾﺎﻤﻧ ﯽﻣ ﺎﻘﻟﺍﺮﺑﺭﺎﮐ ﻪﺑ . ﺪﺘﻓﺍ ﯽﻣ ﻕﺎﻔﺗﺍﺮﻓﺎﺑ pKa ﺎﺑ ﻝﺩﺎﻌﻣ pHﺭﺩ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﻦﯾﺮﺘﻬﺑ ﻪﮐ ﺩﻮﺑ ﺪﻨﻫﺍﻮﺧ ﻝﻮﺒﻗ ﻞﺑﺎﻗ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ یﺍﺭﺍﺩ ﯽﻣﺎﮕﻨﻫ ﺎﻫﺮﻓﺎﺑ ﯽﻠﮐﺭﻮﻃ ﻥﺁ pKa ﺭﺍﺪﻘﻣ ﺯﺍ ﻑﻼﺘﺧﺍ ﺪﺣﺍو ± 1ﺮﺜﮐﺍﺪﺣ ﺎﺑ یﺍ ﻩﺩوﺪﺤﻣﺭﺩ ﺎﻫ ﻥﺁ pH . ﺩﻮﺑﺪﻫﺍﻮﺨﻧ ﻝﻮﺒﻗ ﻞﺑﺎﻗ یﺮﻓﺎﺑ ﺖﯿﻓﺮﻇ ﺕﺭﻮﺼﻨﯾﺍﺮﯿﻏﺭﺩ ،ﺪﺷﺎﺑ ﻪﺘﺷﺍﺩ ﺭﺍﺮﻗ ﺎﻫ . ﺪﻫﺩ ﯽﻣ ﻥﺎﺸﻧ ﺍﺭ ﻥوﺭﺍوﺯﺎﻓ HPLC ﺭﺩ ﻝوﺍﺪﺘﻣ یﺎﻫﺮﻓﺎﺑﺯﺍ ﯽﻀﻌﺑ 1 ﻝوﺪﺟ UV cut off و pH ﻝﺮﺘﻨﮐ یﺍﺮﺑ ﻪﻨﯿﻬﺑ ﻩﺯﺎﺑ ، pKa ﺭﺍﺪﻘﻣ ﻝوﺪﺟ ﻦﯾﺍﺭﺩ . ﺖﺳﺍ ﻩﺪﺷ ﻩﺩﺭوﺁﺰﯿﻧ ﺎﻫﺮﻓﺎﺑ ﺯﺎﺳﺭﺎﮑﺷﺁ ﻪﺑ ﻪﮐﺪﻧﻮﺷ ﯽﻣ ﺖﻓﺎﯾ ﯽﯾﺎﻫ HPLC ﻻﻮﻤﻌﻣ ﻪﮑﯿﯾﺎﺠﻧﺁﺯﺍ ﺰﯿﻧ LC/MS یﺎﻫﺩﺮﺑﺭﺎﮐ یﺍﺮﺑ ﺭﺍﺮﻓ یﺎﻫﺮﻓﺎﺑ ،ﺪﻨﺷﺎﺑ ﻞﺼﺘﻣ MASS . ﺪﻧﺍ ﻩﺪﺷ ﺺﺨﺸﻣ ﯽﻤﮐﺮﯿﺛﺎﺗ ،ﻥوﺭﺍوﺯﺎﻓ HPLC : ﺮﻓﺎﺑ ﺖﻈﻠﻏ ﺭﺩ کﺮﺤﺘﻣﺯﺎﻓ یﺎﻫﺮﻓﺎﺑ ﺖﻈﻠﻏﻻﻮﻤﻌﻣ یﺍﺮﺑ . ﺪﻨﮐ ﻝﺮﺘﻨﮐ ﺍﺭ pH ﻪﮐﺪﺷﺎﺑ یﺪﺣﺭﺩ ﺪﯾﺎﺑ ﻂﻘﻓ و ﺩﺭﺍﺩ یﺭﺍﺩﺯﺎﺑﺭﺩ ﺖﯾﺎﻔﮐﺭﻻﻮﻣ ﯽﻠﯿﻣ 50 ﺎﺗ 25 ﻦﯿﺑ ﺮﻓﺎﺑ ﺖﻈﻠﻏ ،ﻥوﺯﺍوﺯﺎﻓ یﺎﻫﺩﺮﺑﺭﺎﮐﺮﺜﮐﺍ ﺭﺩ ﺏﻮﺳﺭ ﺩﺎﺠﯾﺍ ﺯﺍ ﻪﮐ ﺖﺳﺍ ﻢﮐ ﻥﺎﻨﭼ ﺖﻈﻠﻏ ﻦﯾﺍ ﻝﺎﺣ ﻦﯿﻋ ﺭﺩ . ﺪﻨﮐ ﯽﻣ ﯽﻟﺁ یﺎﻫ ﻩﺪﻨﻨﮐ ﺡﻼﺻﺍ یﺍ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﺭﺍﺪﻘﻣ ﻪﮑﯿﻣﺎﮕﻨﻫ ،ﻢﺘﺴﯿﺳ یﺮﯿﮔﻮﻠﺟ ،ﺪﻧﻮﺷ ﯽﻣ ﻩﺩﺎﻔﺘﺳﺍ کﺮﺤﺘﻣﺯﺎﻓﺭﺩ (Organic Modifiers) ﺮﺛﺍ ﻥﺪﻧﺎﺳﺭ ﻞﻗﺍﺪﺣ ﻪﺑ یﺍﺮﺑ ﻕﻮﻓ ﺖﻈﻠﻏ ﺰﯿﻧ ﺕﺎﻔﺴﻓ ﺮﻓﺎﺑ ﺩﺭﻮﻣﺭﺩ . ﺩﻮﺷ ﺮﺘﻤﮐ ﺖﻈﻠﻏ ﺎﺑﺮﻓﺎﺑﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ . ﺖﺳﺍ ﺐﺳﺎﻨﻣ ﭗﻤﭘ یﺎﻫ seal یوﺭ ﯽﺒﯾﺮﺨﺗ . ﺖﺳﺍ ﻩﺪﺷ ﺵﺭﺎﻔﺳ ﺕﺭﺪﻧ ﻪﺑ ﺭﻻﻮﻣ ﯽﻠﯿﻣ 10ﺯﺍ

ﺎﺗ ﺩﺭﺍﺩ CH3OH/Water ﺭﺩ یﺮﺘﺸﯿﺑ ﺖﯿﻟﻼﺣ ﺕﺎﻔﺴﻓ HPLC THF/ Water ﺎﯾ یﺍﺮﺑ کﺮﺤﺘﻣﺯﺎﻓﺮﻓﺎﺑ ﺏﺎﺨﺘﻧﺍ یﺍﺮﺑ ﺕﺎﻈﺣﻼﻣ ﯽﺧﺮﺑ – 2 ﻝوﺪﺟ ﻥوﺭﺍوﺯﺎﻓ CH3CN/ Water ﺭﺩ ﯽﻟﺁ کﺮﺤﺘﻣ یﺎﻫﺯﺎﻓﺭﺩ یﺮﺘﺸﯿﺑ ﺖﯿﻟﻼﺣ NH یﺎﻬﮑﻤﻧ 4 ﻪﺑ ﺖﺒﺴﻧ ﺰﯿﻧ ﺎﻫ ﻥﺁ و ﻢﯿﺳﺎﺘﭘ یﺎﻬﮑﻤﻧ ﺎﺗﺪﻧﺭﺍﺩ ﯽﺑﺁ / . ﺪﻧﺭﺍﺩ یﺮﺘﺸﯿﺑ ﺖﯿﻟﻼﺣ ﻢﯾﺪﺳ یﺎﻬﮑﻤﻧ ﺏﺬﺟ و ﻩﺩﺮﮐ ﺮﯿﯿﻐﺗ ﻥﺎﻣﺯ ﺖﺷﺬﮔﺮﺛﺍﺭﺩ TEA و TFA ﺮﻓﺎﺑوﺩ ﻦﯾﺍ ﻞﻣﺎﺷ کﺮﺤﺘﻣ یﺎﻫﺯﺎﻓ . ﺩوﺭ ﯽﻣ ﻻﺎﺑ ﺎﻬﻧﺁ UV . ﺪﻧﻮﺷ ﻪﯿﻬﺗ ﻩﺯﺎﺗ ﺪﯾﺎﺑ ﺯﺍ ﺲﭘ . ﺪﻧﺮﻀﻣ ﻞﯿﺘﺳﺍ یﺎﻫ ﻪﻟﻮﻟ یﺍﺮﺑ ﺕﺍﺮﺘﯿﺳ یﺎﻫﺮﻓﺎﺑ ﺍﺭ ﺎﻫ ﻥﺁ ﻪﮐﺪﯾﻮﺷ ﻦﺌﻤﻄﻣ ﺎﻤﺘﺣ ﺎﻫﺮﻓﺎﺑ ﻦﯾﺍﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ . ﺪﯾﺍ ﻩﺩﺮﮐ ﺝﺭﺎﺧ ﺎﻫﺮﯿﺴﻣ ﻪﯿﻠﮐﺯﺍ یﺎﻫ ﻩﺪﻨﻨﮐ ﺡﻼﺻﺍ ﺭﺍﺪﻘﻣ ﻪﮐ ﯽﯾﺎﻫﺮﻓﺎﺑ ﺭﺩ ﯽﺑوﺮﮑﯿﻣ ﺪﺷﺭ ﺖﻋﺮﺳ ﻪﺑ ﺪﻨﺘﺴﻫ ﻥﺁﺪﻗﺎﻓ " ﻼﺻﺍ ﺎﯾو ﻩﺩﻮﺑ ﻢﮐﺎﻫ ﻥﺁ ﯽﻟﺁ ﻪﺘﺷﺎﺒﻧﺍ ﻥﻮﺘﺳ یﺩوﺭو ﺭﺩ ﺪﻧﺍﻮﺗ ﯽﻣ ﺪﺷﺭ ﻦﯾﺍ . ﺪﻫﺩ ﯽﻣ ﺥﺭ کﺮﺤﺘﻣ یﺎﻫﺯﺎﻓ ﻦﯾﺍ . ﺩﺮﺒﺑ ﻦﯿﺑﺯﺍ ﺍﺭ ﻥﻮﺘﺳ ﯽﯾﺍﺭﺎﮐو ﻩﺪﺷ ﺪﯾﺎﺑ ﺎﻬﻧﻮﺘﺳ ﺖﻇﺎﻔﺣ یﺍﺮﺑو ﺪﻧﻮﺷ ﻪﯿﻬﺗ ﻩﺯﺎﺗ و ﻪﻧﺍﺯوﺭ ﺪﯾﺎﺑ .( 4 ﻞﮑﺷ ) ﺩﺮﮐ ﻩﺩﺎﻔﺘﺳﺍ ﻥﻮﺘﺳ ﺶﯿﭘ ﺯﺍ کﺮﺤﺘﻣﺯﺎﻓ ﻪﯿﻬﺗ یﺍﺮﺑ HPLC Grade ﺏﺁﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﻪﺑ ﺪﻧﺍﻮﺗ ﯽﻣ ﻝﺎﭽﺨﯾ یﺎﻣﺩﺭﺩ ﻥﺁ یﺭﺍﺪﻬﮕﻧ و یﺮﻓﺎﺑ . ﺪﻨﮐ ﮏﻤﮐ ﯽﺑوﺮﮑﯿﻣ ﺪﺷﺭ ﻞﮑﺸﻣ ﺶﻫﺎﮐ ﺍﺭ ﺎﮑﯿﻠﯿﺳ ﺐﯾﺮﺨﺗ ﺕﺎﻔﺴﻓ یﺎﻫﺮﻓﺎﺑ ، 7 ﺯﺍ ﺮﺗﻻﺎﺑ pH ﺭﺩ ﯽﻣ ﻢﮐ ﺕﺪﺷ ﻪﺑ ﺍﺭ ﺎﻫ ﻥﺁﺮﻤﻋ ﻝﻮﻃ و ﺪﻨﻫﺩ ﯽﻣ ﺶﯾﺍﺰﻓﺍ ﺭﺎﯿﺴﺑ یﺎﮑﯿﻠﯿﺳ ﺎﺑ یﺎﻫ ﻥﻮﺘﺳ ﺯﺍ ﺖﺳﺍ ﺮﺘﻬﺑ ﺍﺬﻟ . ﺪﻨﻨﮐ .( 5 ﻞﮑﺷ) ﺪﯿﻨﮐ ﻩﺩﺎﻔﺘﺳﺍ ﺺﻟﺎﺧ - - - - - - - 4 . ﺪﯿﯾﺎﻤﻧ ﻢﯿﻈﻨﺗﺯﺎﯿﻧ ﺕﺭﻮﺻﺭﺩ و ﻩﺩﺮﮐ یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﺍﺭ ﻝﻮﻠﺤﻣ pH ﺲﭘ ﻝﻮﻠﺤﻣ ﺎﺗ ﺪﯿﻨﮐﺮﺒﺻ ﯽﻓﺎﮐ ﻩﺯﺍﺪﻧﺍ ﻪﺑ ﻝﻮﻠﺤﻣ pH ﻢﯿﻈﻨﺗ ﻥﺎﻣﺯ ﺍﺭ pH ﺲﭙﺳ . ﺪﺳﺮﺑ ﻝﺩﺎﻌﺗ ﺖﻟﺎﺣ ﻪﺑﺯﺎﺑ ﺎﯾ ﺪﯿﺳﺍ ﻥﺩﺮﮐ ﻪﻓﺎﺿﺍﺯﺍ . ﺪﯿﻨﮐ یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﺪﻨﻧﺎﻣ ،ﯽﻟﺁ ﻩﺪﻨﻨﮐ ﺡﻼﺻﺍ ﺺﺨﺸﻣﺭﺍﺪﻘﻣ ﺎﺑ ﺍﺭ ﺏﺁﺭﺩ ﻩﺪﺷ ﻞﺣﺮﻓﺎﺑ -4 کﺮﺤﺘﻣ ﺯﺎﻓ ﻪﺑ pH pH ، ﺏﺁﺭﺩ ﻩﺪﺷ ﻞﺣﺮﻓﺎﺑ ﻥﺪﯿﺳﺭ یﺍﺮﺑ ، ﻞﯾﺮﺘﯿﻧﻮﺘﺳﺍ ﺎﯾ ﻞﻧﺎﺘﻣ . ﺪﯿﻨﮐ ﻁﻮﻠﺨﻣﺮﻈﻧﺩﺭﻮﻣ pH یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ یﺎﺟ ﻪﺑ -3 ﺪﯿﻧﺍﻮﺗ ﯽﻣ ﺎﻤﺷ یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﻪﮐﺪﻨﭼﺮﻫ ﺪﯿﻨﮐ یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﺍﺭ ﯽﯾﺎﻬﻧ کﺮﺤﺘﻣﺯﺎﻓ ﺖﺴﯿﻧ ﻖﯿﻗﺩ ﺺﻟﺎﺧ ﯽﺑﺁﺮﻓﺎﺑ pH یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﺪﻨﻧﺎﻣ ﯽﻟﺁو ﯽﺑﺁﺮﻓﺎﺑ ﻁﻮﻠﺨﻣ ﻩﺪﻨﻨﮐ ﺡﻼﺻﺍ ﻥﺩوﺰﻓﺍﺯﺍ ﻞﺒﻗ ﺍﺭ pH ﻪﮐ ﺩﻮﺷ ﯽﻣ ﻪﯿﺻﻮﺗ ﺕﺪﺷ ﻪﺑ ﻦﯾﺍﺮﺑﺎﻨﺑ . ﺪﯿﻨﮐ ﻢﯿﻈﻨﺗو یﺮﯿﮔ ﻩﺯﺍﺪﻧﺍ ﯽﻟﺁ ﺪﯾﺮﯿﮔ ﯽﻣ ﻥﺁ pH ﻢﯿﻈﻨﺗ ﻪﺑ ﻢﯿﻤﺼﺗ و ﻩﺩﺮﮐ ﻪﯿﻬﺗﺍﺭﺮﻓﺎﺑ ﻪﮐﺭﺎﺑﺮﻫ ﻝﺎﺣﺮﻫ ﻪﺑ . ﺪﯿﻫﺩ ﻡﺎﺠﻧﺍ ﻦﯿﻌﻣ ﺵوﺭ ﮏﯾ ﻪﺑ ﺍﺭ ﺭﺎﮐ ﻦﯾﺍ ﻩﺭﺍﻮﻤﻫ ﻪﮐ ﺪﯿﻨﮐ ﺖﻗﺩ ﺪﯾﺎﺑ ﻩﺩﺎﻔﺘﺳﺍ ﻥﻮﺘﺳ ﺶﯿﭘﺯﺍ ﯽﺑوﺮﮑﯿﻣ ﺪﺷﺭ ﻞﮑﺸﻣ ﺶﻫﺎﮐ یﺍﺮﺑ – 4 ﻞﮑﺷ ﺪﯿﻨﮐ ﺮﺘﻫﺎﺗﻮﮐ ﺪﺻﺭﺩ 40 ﻝﻮﻃ ﺎﺑ ﯽﻧﻮﺘﺳ ﺯﺍ ﻢﯿﻧﺍﻮﺘﺑ ﻪﮐ ﺩﻮﺷ ﯽﻣ ﺐﺒﺳ ﻥوﺮﮑﯿﻣ 5 یﺎﺟ ﻪﺑ ﻥوﺮﮑﯿﻣ 3 ﺕﺍﺭﺫ ﺎﺑ ﻥﻮﺘﺳ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﺪﯿﻧﺍﺩ ﯽﻣ ﺎﯾﺁ . ﻝﻼﺣ ﻑﺮﺼﻣ ﺭﺩ ﯽﯾﻮﺟ ﻪﻓﺮﺻ ﺪﺻﺭﺩ 40 ﯽﻨﻌﯾ ﻦﯾﺍ ﻪﮐ ﺩﻮﺷ ﯽﻣ ﺮﺘﻫﺎﺗﻮﮐ ﺪﺻﺭﺩ 40 ﺰﯿﻟﺎﻧﺁ ﻥﺎﻣﺯ ﻪﺠﯿﺘﻧ ﺭﺩ و ﻢﯿﻨﮐ ﻩﺩﺎﻔﺘﺳﺍ

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