pGST-MOLUC - Boneandcancer.org

Name of Vector: pGST-MOLUCAntibiotic Selection: AmpCreated by: Tao Feng(He Lab @ The University of Chicago)Date of Creation: September 2001Nar I 4336EcoR V 4146Bsu36 I 4789BspM I 63 Msc I 466BstB I 656PtacBamH I 935Not I 942Sac II 942Xho I 948HinD III 954BssH II 4107GSTNhe I 960Bgl II 967Apa I 3907lac IqSal I 973BstE II 3877Mlu I 3696pGST-MOLUC4996 bpAcc I 974Kpn I 983Xba I 985EcoR I 989Amp RAat II 1273PflM I 3278AlwN I 2671BR322 OriBsa I 2126Pst I 1950Bsp1286 I 1609Linker Sequence901 921 941| | Bam HI |ATCCTCCAAAATCGGATCTGATCGAAGGTCGTGGGATCCGCGGCCGCTCGAGAAGCTTGCH P P K S D L I E G R G I R G R S R S L961 981 1001| | Eco RI |TAGCGAGATCTGTCGACGGTACCTCTAGAATTCATCGTGACTGACTGACGATCTGCCTCGL A R S V D G T S R I H R D -

pGST-MOLUC SequenceAGCTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTG 60GTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCCCGT 120TCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGC 180TGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCA 240CACAGGAAACAGTATTCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC 300AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGC 360GCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATC 420TTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATA 480TAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGC 540TTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT 600TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAG 660ATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGT 720TGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAA 780AATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAAT 840CCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC 900ATCCTCCAAAATCGGATCTGATCGAAGGTCGTGGGATCCgcggccgctcgagaagcttgc 960tagcgagatctgtcgacggtacctctagAATTCATCGTGACTGACTGACGATCTGCCTCG 1020CGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAG 1080CTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG 1140GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATAATTCTT 1200GAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGG 1260TTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTAT 1320TTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTC 1380AATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCT 1440TTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAG 1500ATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTA 1560AGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTC 1620TGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCA 1680TACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGG 1740ATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGG 1800

CCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACA 1860TGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAA 1920ACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAA 1980CTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA 2040AAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAT 2100CTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC 2160CCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATA 2220GACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTT 2280ACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGA 2340AGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAG 2400CGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAA 2460TCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAG 2520AGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTG 2580TCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT 2640ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTA 2700CCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGG 2760GTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGC 2820GTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAA 2880GCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATC 2940TTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGT 3000CAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCT 3060TTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACC 3120GTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCG 3180AGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGT 3240GCGGTATTTCACACCGCATAAATTCCGACACCATCGAATGGTGCAAAACCTTTCGCGGTA 3300TGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGT 3360TATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACC 3420AGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGA 3480ATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCG 3540TTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTC 3600GCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG 3660

CCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACT 3720ATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGT 3780TATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACG 3840GTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAG 3900CGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCA 3960CTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTT 4020TTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCA 4080ACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTG 4140CGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGT 4200TAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGC 4260AACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAA 4320GAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCAT 4380TAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATT 4440AATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGT 4500ATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGAT 4560TACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCA 4620ACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCG 4680CACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTT 4740TCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATAC 4800TGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGT 4860AACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTA 4920CTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTT 4980TGATGGCGTTGGAATT 4996

pGST-MOLUC Restriction SitesUnique enzymes in pGST-MOLUC:BspM I ACCTGC 10/14 63Msc I TGG|CCA 466BsiC I TT`CG,AA 656BstB I TT`CG,AA 656BamH I G`GATC,C 935Sac II CC,GC`GG 942Not I GC`GGCC,GC 942Eag I C`GGCC,G 942Xma III C`GGCC,G 942Ava I C`YCGR,G 948PaeR7 I C`TCGA,G 948Xho I C`TCGA,G 948HinD III A`AGCT,T 954Nhe I G`CTAG,C 960Bgl II A`GATC,T 967Sal I G`TCGA,C 973Acc I GT`MK,AC 974Acc65 I G`GTAC,C 979Asp718 G`GTAC,C 979Kpn I G,GTAC`C 983Xba I T`CTAG,A 985EcoR I G`AATT,C 989BsaA I YAC|GTR 1174Aat II G,ACGT`C 1273Bsp1286 I G,DGCH`C 1609Pst I C,TGCA`G 1950Bsa I GGTCTC 7/11 2126Ahd I GACNN,N`NNGTC 2192AlwN I CAG,NNN`CTG 2671PflM I CCAN,NNN`NTGG 3278Mlu I A`CGCG,T 3696BstE II G`GTNAC,C 3877Bsp120 I G`GGCC,C 3903Apa I G,GGCC`C 3907Ban II G,RGCY`C 3907BssH II G`CGCG,C 4107EcoR V GAT|ATC 4146Hpa I GTT|AAC 4202Kas I G`GCGC,C 4335Nar I GG`CG,CC 4336Ehe I GGC|GCC 4337Bbe I G,GCGC`C 4339Bsu36 I CC`TNA,GG 4789Number of enzymes = 43The following enzymes do not cut in pGST-MOLUC:Afl II Age I Asc I Avr II Blp IBsaB I BseR I BsiW I Bsm I BspM IIBsrG I Bst1107 I Cla I Dra III Eco47 IIIEco72 I Esp I Fse I Mun I Nae INco I Nde I NgoM I Nru I Nsi IPme I Pml I PpuM I PspA I Rsr IISac I Sfi I Sma I SnaB I Spe ISph I Spl I Srf I Stu I Sty IpGST-MOLUC: sites sorted by name:Aat II (1) 1273Acc I (1) 974Acc65 I (1) 979Aci I (59) 226 550 939 941945 1093 1132 11421184 1301 1348 14471556 1633 1677 17981844 2035 2126 24882497 2632 2742 28632882 3009 3037 31283149 3156 3199 32163242 3255 3296 34083447 3463 3472 34953514 3586 3650 36703673 3724 3901 39854141 4195 4251 42794352 4362 4418 45214679 4755 4884Afl III (3) 497 3080 3696Aha II (5) 36 1270 1652 36534336Ahd I (1) 2192Alu I (27) 3 56 180 484633 811 956 10621081 1839 1902 20022523 2780 2826 29163142 3477 3757 41814296 4389 4453 45484657 4768 4948Alw I (11) 922 931 942 15521558 1873 2337 23382435 2435 2521AlwN I (1) 2671Apa I (1) 3907ApaL I (4) 19 1520 2766 3676Apo I (3) 989 3261 3971Ase I (4) 191 2017 4381 4440Asp718 (1) 979Ava I (1) 948Ava II (4) 753 1828 2050 4248BamH I (1) 935Ban I (7) 979 2239 3616 43354465 4745 4757Ban II (1) 3907Bbe I (1) 4339Bbs I (3) 1209 3842 4181Bbv I (11) 55 1071 1168 19602149 2751 3170 31884398 4647 4720Bbv II (3) 1208 3841 4180Bcl I (2) 693 3710Bcn I (7) 1067 1102 1657 20082704 3317 4126Bfa I (6) 271 961 986 19992334 2587Bgl I (2) 2074 4717Bgl II (1) 967Bpm I (4) 2123 3534 4023 4794Bsa I (1) 2126BsaA I (1) 1174BsaH I (5) 36 1270 1652 36534336BsaJ I (6) 939 2920 4331 44704607 4897BsaW I (4) 1896 2727 2874 4015Bsg I (5) -3 91 545 35473747BsiC I (1) 656BsiE I (6) 945 1674 1823 27463170 4688BsiHKA I (5) 23 1524 1609 27703680BsmA I (7) 1064 1351 2125 33923797 3923 4310BsmB I (2) 1063 4311BsmF I (2) 246 4794BsoF I (30) 44 942 945 10601157 1448 1677 17721799 1949 2138 24662672 2675 2740 28833038 3156 3159 31773464 3587 3651 36713758 4129 4258 43874636 4709Bsp120 I (1) 3903Bsp1286 I (1) 1609BspH I (3) 1247 1352 2360BspM I (1) 63Bsr I (20) 1168 1546 1718 19852028 2146 2552 26662679 3353 3514 35523616 3853 4005 43154414 4575 4601 4823BsrB I (6) 226 550 947 13503151 4521BsrD I (4) 1958 2132 3743 4109BssH II (1) 4107BssS I (3) 1216 1523 2907BstB I (1) 656BstE II (1) 3877BstN I (11) 766 2921 2934 30553421 3736 4276 43334472 4609 4736BstU I (27) 362 941 1021 10231126 1301 1633 21262456 3037 3296 34103447 3497 3568 35863602 3691 3698 38463892 3927 4109 41334364 4366 4970BstX I (3) 3498 3627 3750BstY I (9) 915 935 967 15451562 2330 2342 24282439Bsu36 I (1) 4789Cac8 I (30) 524 631 874 878958 962 1946 20792470 3030 3067 31533426 3516 3566 36213762 3903 3942 39464109 4228 4255 43914420 4655 4659 46794770 4950Cfr10 I (2) 2107 3382Csp6 I (4) 831 980 1710 3842Dde I (8) 625 1265 1691 22312397 2806 4268 4789Dpn I (25) 662 695 917 922937 969 1012 15111547 1564 1822 18681886 2227 2332 23442422 2430 2441 25163608 3712 4085 46874783DpnII (25) 660 693 915 920935 967 1010 15091545 1562 1820 18661884 2225 2330 23422420 2428 2439 25143606 3710 4083 46854781Dra I (5) 686 795 1614 2306

2325Drd I (2) 1115 2978Dsa I (2) 939 4897Eae I (5) 464 942 1799 43704573Eag I (1) 942Ear I (5) 338 1393 3197 33144666Eco57 I (2) 1525 2539EcoN I (2) 269 1831EcoO109 I (2) 291 1212EcoR I (1) 989EcoR II (11) 764 2919 2932 30533419 3734 4274 43314470 4607 4734EcoR V (1) 4146Ehe I (1) 4337Fnu4H I (30) 44 942 945 10601157 1448 1677 17721799 1949 2138 24662672 2675 2740 28833038 3156 3159 31773464 3587 3651 36713758 4129 4258 43874636 4709Fok I (10) 692 781 887 11081753 2040 2221 37423751 4627Fsp I (2) 1969 4707Gdi II (5) 941 943 1800 43694572Gsu I (4) 2122 3535 4022 4793Hae I (7) 466 861 2606 30583069 3424 4963Hae II (6) 2840 3210 3315 40964339 4730Hae III (21) 293 466 861 9441214 1801 2068 21482606 3040 3058 30693424 3559 3905 42744372 4575 4677 47944963Hga I (11) 26 735 1116 16592391 2969 3643 36883917 3923 4975HgiA I (5) 23 1524 1609 27703680HgiE II (3) 2491 3287 4746Hha I (34) 112 141 362 10231126 1156 1301 16331970 2063 2456 25652739 2839 2906 31763209 3314 3570 36043693 3894 3927 40954102 4109 4111 41334338 4366 4431 47084729 4844HinC II (4) 185 975 1650 4202Hind II (4) 185 975 1650 4202HinD III (1) 954Hinf I (9) 2193 2710 3106 31813324 4120 4375 45664904HinI I (5) 36 1270 1652 36534336HinP I (34) 110 139 360 10211124 1154 1299 16311968 2061 2454 25632737 2837 2904 31743207 3312 3568 36023691 3892 3925 40934100 4107 4109 41314336 4364 4429 47064727 4842Hpa I (1) 4202Hpa II (19) 1066 1100 1655 18972007 2074 2108 25122702 2728 2875 33163383 3775 4016 41244493 4743 4761Hph I (15) 381 450 702 10411050 1466 1499 17072121 2348 3350 34253872 4292 4325Kas I (1) 4335Kpn I (1) 983Mae I (6) 271 961 986 19992334 2587Mae II (12) 474 801 883 11731270 1590 1963 23793358 3431 4589 4858Mae III (19) 699 997 1074 11691531 1719 1872 19302261 2544 2660 27233354 3877 4593 46134833 4859 4917Mbo I (25) 660 693 915 920935 967 1010 15091545 1562 1820 18661884 2225 2330 23422420 2428 2439 25143606 3710 4083 46854781Mbo II (17) 342 354 413 6691213 1409 1518 15962351 2424 3213 33303677 3846 4185 46824777Mlu I (1) 3696Mme I (5) 2688 2872 3289 49314971Mnl I (19) 913 992 1026 10561224 1819 2025 21712252 2652 2903 29763186 3557 4041 43644667 4784 4822Msc I (1) 466Mse I (23) 191 287 446 451685 794 1241 16131978 2017 2252 23052319 2324 2376 35933716 3910 4201 43814440 4624 4933Msl I (5) 353 1421 1780 37484036Msp I (19) 1066 1100 1655 18972007 2074 2108 25122702 2728 2875 33163383 3775 4016 41244493 4743 4761MspA1 I (9) 941 1132 1556 24972742 3726 4296 43894657Nar I (1) 4336Nci I (7) 1066 1101 1656 20072703 3316 4125Nhe I (1) 960Nla III (22) 261 501 700 720753 860 1059 11641251 1356 1749 17851863 1873 2364 30843308 3835 4014 40354187 4559Nla IV (16) 755 937 981 13051895 2106 2147 22413013 3052 3618 39054337 4467 4747 4759Not I (1) 942Nsp7524 I (3) 497 1055 3080NspB II (9) 941 1132 1556 24972742 3726 4296 43894657NspH I (3) 501 1059 3084PaeR7 I (1) 948Pal I (21) 293 466 861 9441214 1801 2068 21482606 3040 3058 30693424 3559 3905 42744372 4575 4677 47944963PflM I (1) 3278Ple I (4) 2201 3189 3332 4128Psp1406 I (3) 1590 1963 3358Pst I (1) 1950Pvu I (2) 1823 4688Pvu II (3) 4296 4389 4657Rsa I (4) 832 981 1711 3843Sac II (1) 942Sal I (1) 973Sap I (2) 338 3197Sau3A I (25) 660 693 915 920935 967 1010 15091545 1562 1820 18661884 2225 2330 23422420 2428 2439 25143606 3710 4083 46854781Sau96 I (13) 291 753 1212 18282050 2067 2146 35583903 3904 4248 42724676Sca I (2) 832 1711ScrF I (18) 766 1066 1101 16562007 2703 2921 29343055 3316 3421 37364125 4276 4333 44724609 4736Sec I (6) 939 2920 4331 44704607 4897SfaN I (14) 760 1087 1492 17391932 2984 3204 32423730 3869 4059 40604817 4830Sfc I (4) 1946 2624 2815 4953Ssp I (2) 167 1387Taq I (12) 8 309 577 656923 949 974 15382982 3275 3634 3656Tfi I (4) 3106 4375 4566 4904Tsp45 I (7) 997 1074 1169 17191930 3877 4593Tth111 I (2) 1167 3522Tth111 II (7) 504 530 2458 24882497 3534 4227Vsp I (4) 191 2017 4381 4440Xba I (1) 985Xcm I (3) 3552 4068 4086Xho I (1) 948Xho II (9) 915 935 967 15451562 2330 2342 2428

2439Xma III (1) 942Site usage in pGST-MOLUC:Xmn I (2) 652 1592Aat II G,ACGT`C 1 Acc I GT`MK,AC 1Acc65 I G`GTAC,C 1 Aci I C`CG,C 59Afl II C`TTAA,G - Afl III A`CRYG,T 3Age I A`CCGG,T - Aha II GR`CG,YC 5Ahd I GACNN,N`NNGTC 1 Alu I AG|CT 27Alw I GGATC 8/9 11 AlwN I CAG,NNN`CTG 1Apa I G,GGCC`C 1 ApaL I G`TGCA,C 4Apo I R`AATT,Y 3 Asc I GG`CGCG,CC -Ase I AT`TA,AT 4 Asp718 G`GTAC,C 1Ava I C`YCGR,G 1 Ava II G`GWC,C 4Avr II C`CTAG,G - BamH I G`GATC,C 1Ban I G`GYRC,C 7 Ban II G,RGCY`C 1Bbe I G,GCGC`C 1 Bbs I GAAGAC 8/12 3Bbv I GCAGC 13/17 11 Bbv II GAAGAC 7/11 3Bcl I T`GATC,A 2 Bcn I CC,S`GG 7Bfa I C`TA,G 6 Bgl I GCCN,NNN`NGGC 2Bgl II A`GATC,T 1 Blp I GC`TNA,GC -Bpm I CTGGAG 22/20 4 Bsa I GGTCTC 7/11 1BsaA I YAC|GTR 1 BsaB I GATNN|NNATC -BsaH I GR`CG,YC 5 BsaJ I C`CNNG,G 6BsaW I W`CCGG,W 4 BseR I GAGGAG 16/14 -Bsg I GTGCAG 22/20 5 BsiC I TT`CG,AA 1BsiE I CG,RY`CG 6 BsiHKA I G,WGCW`C 5BsiW I C`GTAC,G - Bsm I GAATG,C 7 -BsmA I GTCTC`/9 7 BsmB I CGTCTC 7/11 2BsmF I GGGAC 15/19 2 BsoF I GC`N,GC 30Bsp120 I G`GGCC,C 1 Bsp1286 I G,DGCH`C 1BspH I T`CATG,A 3 BspM I ACCTGC 10/14 1BspM II T`CCGG,A - Bsr I ACT,GG` 20BsrB I GAG|CGG 6 BsrD I GCAATG, 8 4BsrG I T`GTAC,A - BssH II G`CGCG,C 1BssS I C`TCGT,G 3 Bst1107 I GTA|TAC -BstB I TT`CG,AA 1 BstE II G`GTNAC,C 1BstN I CC`W,GG 11 BstU I CG|CG 27BstX I CCAN,NNNN`NTGG 3 BstY I R`GATC,Y 9Bsu36 I CC`TNA,GG 1 Cac8 I GCN|NGC 30Cfr10 I R`CCGG,Y 2 Cla I AT`CG,AT -Csp6 I G`TA,C 4 Dde I C`TNA,G 8Dpn I GA|TC 25 DpnII `GATC, 25Dra I TTT|AAA 5 Dra III CAC,NNN`GTG -Drd I GACNN,NN`NNGTC 2 Dsa I C`CRYG,G 2Eae I Y`GGCC,R 5 Eag I C`GGCC,G 1Ear I CTCTTC 7/10 5 Eco47 III AGC|GCT -Eco57 I CTGAAG 21/19 2 Eco72 I CAC|GTG -EcoN I CCTNN`N,NNAGG 2 EcoO109 I RG`GNC,CY 2EcoR I G`AATT,C 1 EcoR II `CCWGG, 11EcoR V GAT|ATC 1 Ehe I GGC|GCC 1Esp I GC`TNA,GC - Fnu4H I GC`N,GC 30Fok I GGATG 14/18 10 Fse I GG,CCGG`CC -Fsp I TGC|GCA 2 Gdi II `YGGC,CG 5Gsu I CTGGAG 21/19 4 Hae I WGG|CCW 7Hae II R,GCGC`Y 6 Hae III GG|CC 21Hga I GACGC 9/14 11 HgiA I G,WGCW`C 5HgiE II ACCNNNNNNGGT -1/133 Hha I G,CG`C 34HinC II GTY|RAC 4 Hind II GTY|RAC 4HinD III A`AGCT,T 1 Hinf I G`ANT,C 9HinI I GR`CG,YC 5 HinP I G`CG,C 34Hpa I GTT|AAC 1 Hpa II C`CG,G 19Hph I GGTGA 12/11 15 Kas I G`GCGC,C 1Kpn I G,GTAC`C 1 Mae I C`TA,G 6Mae II A`CG,T 12 Mae III `GTNAC, 19Mbo I `GATC, 25 Mbo II GAAGA 12/11 17Mlu I A`CGCG,T 1 Mme I TCCRAC 25/23 5Mnl I CCTC 10/10 19 Msc I TGG|CCA 1Mse I T`TA,A 23 Msl I CAYNN|NNRTG 5Msp I C`CG,G 19 MspA1 I CMG|CKG 9Mun I C`AATT,G - Nae I GCC|GGC -Nar I GG`CG,CC 1 Nci I CC`S,GG 7Nco I C`CATG,G - Nde I CA`TA,TG -NgoM I G`CCGG,C - Nhe I G`CTAG,C 1Nla III ,CATG` 22 Nla IV GGN|NCC 16Not I GC`GGCC,GC 1 Nru I TCG|CGA -Nsi I A,TGCA`T - Nsp7524 I R`CATG,Y 3NspB II CMG|CKG 9 NspH I R,CATG`Y 3PaeR7 I C`TCGA,G 1 Pal I GG|CC 21PflM I CCAN,NNN`NTGG 1 Ple I GAGTC 9/10 4Pme I CTTT|AAAC - Pml I CAC|GTG -PpuM I RG`GWC,CY - Psp1406 I AA`CG,TT 3PspA I C`CCGG,G - Pst I C,TGCA`G 1Pvu I CG,AT`CG 2 Pvu II CAG|CTG 3Rsa I GT|AC 4 Rsr II CG`GWC,CG -Sac I G,AGCT`C - Sac II CC,GC`GG 1Sal I G`TCGA,C 1 Sap I GCTCTTC 8/11 2Sau3A I `GATC, 25 Sau96 I G`GNC,C 13Sca I AGT|ACT 2 ScrF I CC`N,GG 18Sec I C`CNNG,G 6 SfaN I GCATC 9/13 14Sfc I C`TRYA,G 4 Sfi I GGCCN,NNN`NGGCC -Sma I CCC|GGG - SnaB I TAC|GTA -Spe I A`CTAG,T - Sph I G,CATG`C -Spl I C`GTAC,G - Srf I GCCC|GGGC -Ssp I AAT|ATT 2 Stu I AGG|CCT -Sty I C`CWWG,G - Taq I T`CG,A 12Tfi I G`AWT,C 4 Tsp45 I `GTSAC, 7Tth111 I GACN`N,NGTC 2 Tth111 II CAARCA 16/14 7Vsp I AT`TA,AT 4 Xba I T`CTAG,A 1Xca I GTA|TAC - Xcm I CCANNNN,N`NNNNTGG3Xho I C`TCGA,G 1 Xho II R`GATC,Y 9Xma I C`CCGG,G - Xma III C`GGCC,G 1

pGST-MOLUC GST Coding Region1 21 41| | |AGCTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTG61 81 101| | |GTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCCCGT121 141 161| | |TCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGC181 201 221| | |TGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCA241 261 281| | |CACAGGAAACAGTATTCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCM S P I L G Y W K I K G L V301 321 341| | |AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCQ P T R L L L E Y L E E K Y E E H L Y E361 381 401| | |GCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCR D E G D K W R N K K F E L G L E F P N421 441 461| | |TTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATAL P Y Y I D G D V K L T Q S M A I I R Y481 501 521| | |TAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCI A D K H N M L G G C P K E R A E I S M541 561 581| | |TTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTL E G A V L D I R Y G V S R I A Y S K D601 621 641| | |TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGF E T L K V D F L S K L P E M L K M F E

661 681 701| | |ATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTD R L C H K T Y L N G D H V T H P D F M721 741 761| | |TGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAL Y D A L D V V L Y M D P M C L D A F P781 801 821| | |AATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATK L V C F K K R I E A I P Q I D K Y L K841 861 881| | |CCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCS S K Y I A W P L Q G W Q A T F G G G D901 921 941| | |ATCCTCCAAAATCGGATCTGATCGAAGGTCGTGGGATCCGCGGCCGCTCGAGAAGCTTGCH P P K S D L I E G R G I R G R S R S L961 981 1001| | |TAGCGAGATCTGTCGACGGTACCTCTAGAATTCATCGTGACTGACTGACGATCTGCCTCGL A R S V D G T S R I H R D -1021 1041 1061| | |CGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAG1081 1101 1121| | |CTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG1141 1161 1181| | |GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATAATTCTT1201 1221 1241| | |GAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGG