download the presentation - EuroBiO 2009

In- and out-sourcing management forsuccessful business developmentEurobio 2009 Lille

Protéus - corporate overview• Founded in 1998• Based in Nîmes, France• Highly skilled multidisciplinary team• In-house IP team• Over 2000 m² research premises (includingclean rooms and a bio-process developmentfacility)Eurobio 2009 Lille

Protéus, world expert in proteinsProteinsBiological propertiesCatalytic propertiesDiscovers, optimizes, producesand formulates proteinsProducts and bioprocesses for industryEurobio 2009 Lille

Proteins for industrial innovationHealthcareChemistryEnergyEnvironmentEurobio 2009 Lille

Integrated PlatformEurobio 2009 Lille

Multidisciplinary platformMicrobiologyMolecular biologyBioinformaticsProtein expressionIntellectual PropertyProtein engineeringProcess developmentChemistryHigh throughput screeningEurobio 2009 Lille

Industrial partnershipsHealthcareChemistryEnergy... ...Environment......Proteins for industrial innovationEurobio 2009 Lille

In- and out-sourcing management forsuccessful business developmentBy entrusting projects to biotech companies, theindustrial companies are looking for a set of:powerful technologies,know-howindustrial property.Eurobio 2009 Lille

From concept to industrial products…How to obtain a protein fulfilling theneeds of its target application?Eurobio 2009 Lille

From the idea to the productStrategic studies for industrial and pharmaceuticalproteinsProtein discovery and developmentProtein engineering and optimisationProtein- or cell-based process developmentBiocatalysts formulationProtein immunogenicity assessmentProtein production and biomanufacturingMarketingSalesEurobio 2009 Lille

Proteus Business ChainBibliographic, Strategicand Feasibility StudiesBiodiversity screeningProtein engineeringProtein expressionBioprocess Scale upManufacturingEurobio 2009 LilleMarketing & Sales

A vast collection of biodiversityfor sourcing new genes and proteinsBiodiversity screeningEurobio 2009 Lille

Protéus biodiversityVery large diversity collection including extremophilepH14Soda lakes and soils(salt accumulationincluding carbonate)ALCALOPHILESPolar areas,ice fields9Temperature (°C)0 20 60 110PSYCHROPHILES MESOPHILES THERMOPHILES305Hydrothermal vents,Solfatara fields,Geysers, Oil fields10 5 10 8HALOPHILESBAROPHILESSalt lakes,salterns, lagoonsSolfataresEurobio 2009 Lille350Salt concentration (g/l)ACIDOPHILESAcid lakes and soils,gastro-intestinal tracts, volcanic env.0Pressure (KPa)Deep sea(barotolerants)

Protéus biodiversity - a unique international networkProminent members of the networkIFREMERIRD (former ORSTOM)Hebrew University of JerusalemGriffith University, AustraliaTotal-Fina-ElfDegussa Ferm. AromatisationBIODEEP (EC-funded program)University of Buenos AiresUniversity of MoscowHERMES (EC-funded program)Biodiversity from:Deep sea hydrothermal ventsAnaerobic environmentsand tropical fermentation(hyper)saline environments(e.g. Dead Sea)Hot non volcanic environments(Great Artesian Basin)Oil fieldsFood ingredientsHypersaline deep sea basins(Mediterranean Sea)Cold environments(Antartica)(Hyper)-ThermophilesEurope’s deep-ocean marginAccess to strains, samples and knowledgeEurobio 2009 Lille

What does screening result into ?Biodiversity screeningProtein engineeringSelection of strains and/or genelibrariesEurobio 2009 Lille

What does protein engineering result into ?Biodiversity screeningProtein engineeringProtein engineeringOptimized genes and proteinsEurobio 2009 Lille

Case study: improving industrial enzymeResults after L-Shuffling and HTS1000900Inactive 26% < WT 34% ≈ WT12% > WT 28 %Relative activity %8007006005004003002001000Parental genesEurobio 2009 LilleNumber of clones screened

Other case studyImprove specific activity 2 L-Shuffling rounds using three homologous genes (70%)Set up of the HTS assay (colorimetric assay)Screening of 25 000 clones per roundEurobio 2009 Lille

ResultsGenetic diversityVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQATAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVASH1:Referenceprotein Xparentalprotein AVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQATAKHYILNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNHVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVFSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYILNEQELNRETISDVDRTMHELYLWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGFGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDALKTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDAKIEPRYEFGFGLSYTEFTYADLSVTSTVTAGPASGETIPGADLWETVATVTASITNSGEVEGAEVAQLYITLPSAPSTPKQLRGFAKLKLEPGASGVATFNLRDLSYWDAGRGQWVPAGEFTVSVGASRDVRLTGSLTAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQATAKHYILNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDEGVLPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKIPHGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYIVNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKINTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYIVNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKINGSWGCEDQYTLQTVLKDQLGFPGYVSDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPSTPKQLRGFAKLKLEPGASGVATFNLRDLSYWDAGRGQWVPAGEFTVSVGASRDVRLTGSLTAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNHVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPGKVALVGSASVNSAGPN-VDKGCNTGALGMGWGSGSVNYPYFVAPYDALKTRAQADGTLSLHNSDSTNGVSGVSGADVAIVITADSGEGYITVEGHAGDRNHLDPWHDGNALVKAVANKNTIVHSTGPILETILATEGVKAVWAGLPSQENGNALVDVLWGDVSPSGKLVYSIAKRPEDYGTAPSKGSNDKFTEGLFVDYRHFDNAKIEPRYEFGFGLSYTEFTYADLSVTSTVTAGPASGETIPGADLWETVATVTASITNSGEVEGAEVAQLYITLPSAPSTPKQLRGFAKLKLEPGASGVATFNLRDLSYWDTASQKWVPSGSFGISVGASRDIRLTGSLTAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQACAKHYIVNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQATAKHYILNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVANKNTIVHSTGPILETILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSVGVQATAKHYILNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKVNTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVASH2:BestSH1 Xparentalprotein CVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVQANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVFSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKIPHGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGSVTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYIVNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGFGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISDVDRTMHELYLWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGVRYSTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYILNEQELNRETISNPDRTLHELYLWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQNWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPHGRNWEGFGTDPYLAGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISNPDRTLHELYLWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDAKIEPRYEFGFGLSYTEFTYADLSVTSTVTAGPASGETIPGADLWETVATVTASITNSGEVEGAEVAQLYITLPSAPSTPKQLRGFAKLKLEPGASGVATFNLRDLSYWDAGRGQWVPAGEFTVSVGASRDVRLTGSLTA-VPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGSVTAFTPGIQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKIPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYIVNEQELNRETISNPDRTLHELYTWPFADAVQANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDEGVLPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYIVNEQELNRETISNPDRTMHELYLWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKIGYPQLCLQDGPLGIRFGSVTAFTPGIQASTWDVELIRQRGVYLGAEARGVGVHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMSETIEGIQSNGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDVNLIRERGQFIGEVKASGIHVILGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYILNEQELNRETISNPDRTMHELYLWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGDSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVILGPVAGPLGKIPHGRNWEGFGVDPYLTGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNQVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGPLGKTPQGRNWEGFGVDPYLTGIAMGQTINGIQSAGVQACAKHYILNEQELNRETISDVDRTLHELYTWPFADAVHANVASVMCSYNKINGSWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNHVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAVPAGTPWGTAYDKAKALAKLNLQDKVGIVSGVGWNGPCVGNTSPASKISYPSLCLQDGPLGIRFGTGSTAFTPGVQASTWDTELMRQRGEYLGAEAKGCGIHVLGPVAGALGKIPQGRNWEGFGVDPYLAGIAMAETIEGLQSAGVQACAKHYILNEQELNRETISNPDRTLHELYLWPFADAVHANVASVMCSYNKVNGTWACEDQYTLQTVLKDQLGFPGYVMTDWNAQHTVQSANSGLDMSMPGTDFNGNRLWGPALTNAVNSNHVPTSRVDMVTRILAWYLTGQDQAGYPSFNISRNVQGNHKTNVRAIARDGIVLKNDANILPLKPASIAVGSAIGNHARNSPSCNDKGCDGALGMGWGSGAVNYPYFVAPYDAINTRASQGTQVTLSNTDNTSGASARGKDVAIVFITADSGEGYITVEGNAGDRNLDPWHNGNALVQAVAGANSNVIVHSVGAILEQILALPQVKAVWAGLPSQESGNALVDVLWGDVSPSGKLVYTIAKSPNDYNTRIVSGSDSFSEGLFIDYKHFDANITPRYEFGYGLSYTKFNYSRLSVLSTAKSGPATGAVPGPSDLFQNVATVTVDIANSGQVTGAEVAQLYITYPSAPRTPKQLRGFAKLNLTPGQSGTATFNIRDLSYWDTASQKWVPSGSFGISVGASRDIRLTSTLSVAReference protein; parental protein A; parental protein CEurobio 2009 Lille Large sequence diversity Among the best performers, up to 11 recombinationevents per gene were observed

What does protein expression result into ?Biodiversity screeningProtein engineeringProtein engineeringProtein expressionEurobio 2009 LilleMilligrams or grams ofoptimized protein, formulatedproduction and manufacturingas appropriate for theapplication

Host library - Prokaryotes• Multiparallel expression profiling strategy toenhance success• An unmet panel of expression systems andconditions, allowing for expression of mostproteins• Hosts:– Bacteria (E. coli, Bacillus, Pseudomonas)– Yeast (Yarrowia)– Baculovirus / insect cellsEurobio 2009 Lille

Example of expression profilingEurobio 2009 Lille

Key deliverable from ProtéusBiodiversity screeningProtein engineeringProtein engineeringProtein expressionProtein ready to be industrializedEurobio 2009 Lille

In- and out-sourcing management for successfulbusiness developmentBibliographic, Strategicand Feasibility StudiesBiodiversity screeningProtein engineeringProtein expressionScaled up bioprocessesEurobio 2009 LilleProduct and process resulting froma combination of in house andoutsourced R&D

In- and out-sourcing management for successfulbusiness developmentNext stepEurobio 2009 Lille

In- and out-sourcing management for successfulbusiness developmentBibliographic, Strategicand Feasibility StudiesProtéus orR&DpartnersBiodiversity screeningProtein engineeringProtein expressionBioprocess Scale upEurobio 2009 LilleManufacturingMarketing & SalesCustomersor industrialpartners

Relationship Between Owners of Technologies,Protéus & Industrial PartnersThe Deal :Owner of technologies, Know How, IPRscreates value from its technologies, Know How andIndustrial Property RightsProtéuscombines complementary outsourced technologies,know-how and IP with in house R&D to develop newproducts and processes for industrial applicationsIndustrial Partnersmake money by implementing processes and sellingproducts.Eurobio 2009 Lille

In- and out-sourcing management forsuccessful business developmentClauses to be discussed• Definitions• Research Funding• Intellectual Property Rights• Scope• Confidentiality.• Term and Termination• Applicable Law• ………………Eurobio 2009 Lille

The Take Away MessageNo single biotech is able to build such acomprehensive offering unless it teams up with anetwork of academic and industrial third parties toacquire complementary expertise and industrialproperty.Eurobio 2009 Lille

Thank you for your attentionJean-François BLOCHTel: +33 (4) 66 70 64 68jfbloch@proteus.frwww.proteus.frEurobio 2009 Lille