Antigen Retrieval - NordiQC

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HIER procedurerMWO ~ RT / MWO / 75ºC ~ 8 min / 20 min / 10 minPT-Link ~ 85ºC / 95ºC / 85ºC ~ 8 min / 15 min / 8 minPT-Link ~ 85ºC / 95ºC / 85ºC ~ 8 min / 30 min / 8 minPT-Link ~ 85ºC / 97ºC / 85ºC ~ 8 min / 20 min / 10 minSlut titrering:CD5 clone 4C7 1:50 → 1:6400CD23 clone 1B12 1:50 → 1:6400CyclinD1 clone SP4 1:50 → 1:6400PT-Link ~ 65ºC / 97ºC / 65ºC ~ 18 min / 20 min / 32 min (DAKO)
MWO - CD5 1+50 PT-95/15 min. - CD5 1+50 1+6400PT- 95/30 min.CD5 clone 4C7PT- 97/20 min.4,543,5Score32,521,5PT-95/30min.PT-97/20min.PT-95/15min.MWO/TEG/20min.PT- 95/15 min.10,501+800 1+1600 1+3200 1+6400DilutionMWO/20 min.
Page 1 and 2: Antigen RetrievalErfaringer med PT-
Page 3 and 4: 1423NordiQCSøren Nielsen /Aalborg
Page 5 and 6: Hepar/Tonsil fix. 24h/NBFCD34 clone
Page 7 and 8: Knoglemarvs aspirater (koagler) 8 o
Page 9: Effektivitet af forskellige demaske
Page 13 and 14: Epitope RetrievalNatten over ved 75
Page 15 and 16: Epitope Retrieval, PT-Link, High ph
Page 17 and 18: Konklusion• Pre-heat settings 85
Page 19 and 20: Epitope Retrieval, PT-Link, High ph

Antigen Retrieval - NordiQC

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