Antigen Retrieval - NordiQC

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ProblemPT, High-buffer (3-1 buffer)RT/ 95ºC/85ºC ~41/15/8 min65ºC/95ºC/65ºC ~18/30/32 min65ºC/95 or 97ºC/65ºC ~ 18/20/32 min65ºC/95 or 97or 99ºC/65ºC ~ 18/10/32 minMorfologi ”suboptimal”:• Knoglemarvsaspirater / Koagler• Vabrasio uterus• Celle el. vævsmateriale (Akut / kronisk inflammation)•?
1423<strong>NordiQC</strong>Søren Nielsen /Aalborg
Page 1: Antigen RetrievalErfaringer med PT-
Page 5 and 6: Hepar/Tonsil fix. 24h/NBFCD34 clone
Page 7 and 8: Knoglemarvs aspirater (koagler) 8 o
Page 9 and 10: Effektivitet af forskellige demaske
Page 11 and 12: MWO - CD5 1+50 PT-95/15 min. - CD5
Page 13 and 14: Epitope RetrievalNatten over ved 75
Page 15 and 16: Epitope Retrieval, PT-Link, High ph
Page 17 and 18: Konklusion• Pre-heat settings 85
Page 19 and 20: Epitope Retrieval, PT-Link, High ph

Antigen Retrieval - NordiQC

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