pdf✔download⚡ A Practical Textbook of Genetic Engineering in Bacteria
COPY LINK : https://fastpdf.bookcenterapp.com/yump/B00TYWLCO8 Genetic Entropy presents compelling scientific evidence that the genomes of all living creatures are slowly degenerating - due to the accumulation of slightly harmful mutations. This is happening in spite of natural selection. The author of this book, Dr. John Sanford, is a Cornell University geneticist. Dr. Sanford has devoted more than 10 years of his life to the study of this specific problem. Arguably, he has examined this problem in greater depth than any other scientist. The evidences that he presents are diverse and compelli
COPY LINK : https://fastpdf.bookcenterapp.com/yump/B00TYWLCO8
Genetic Entropy presents compelling scientific evidence that the genomes of all living creatures are slowly degenerating - due to the accumulation of slightly harmful mutations. This is happening in spite of natural selection. The author of this book, Dr. John Sanford, is a Cornell University geneticist. Dr. Sanford has devoted more than 10 years of his life to the study of this specific problem. Arguably, he has examined this problem in greater depth than any other scientist. The evidences that he presents are diverse and compelli
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A Practical Textbook of Genetic Engineering in Bacteria
Description :
Genetic manipulation of bacteria is exploited largely in the
laboratory because of their simple genetics and as they are easy to
grow. These bacteria are predominantly important in producing large
amounts of pure recombinant human proteins which can be used in
medicine. The first example of this kind was shown in 1978 by
Herbert Boyer working in a laboratory in University of California. He
expressed human insulin gene in Escherichia coli. This product was
approved by U.S. Food and Drug Administration for treating
diabetes. The ease with which the bacteria could be manipulated has
helped researchers in obtaining important molecules which otherwise
are very difficult to purify from the organism in large amounts to be
used in therapeutic purposes. The genetic sequences from a broad
range of organisms can be easily ligated to a plasmid and
transformed into bacteria for both storage and modification. Bacteria
are simple to grow, divide quickly, easy to transform, and can be
stored at -86 °Cmore or less indefinitely. The moment a gene
is isolated it is very easy to store in bacteria and this provides
limitless supply of active material for research. This has led to the
development of huge number of recombinant plasmids for
manipulating DNA/genes of different prokaryotic and eukaryotic
organisms. CONTENTS: Chapter 1 - Cloning of DNA Fragments into
Plasmid Vectors, Chapter 2 - Cloning of DNA fragments into Phage
Vectors, Chapter 3 - Cloning in cosmid vectors, Chapter 4 -
Techniques to understand the functionality of a gene, Glossary,
Index.
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