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<strong>Generon</strong> Ltd., 12 Rawcliffe House,<br />
Howarth Road, Maidenhead,<br />
Berkshire SL6 1AP.<br />
Order Tel: (01753) 866-511<br />
Order Fax: (01753) 20-88-99<br />
Order E-mail: info@generon.co.uk<br />
Web: www.generon.co.uk<br />
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Vivascience Vivapure Price List<br />
Ion exchange membrane type Protein binding capacity<br />
C = Carboxyl groups, weak cation exchanger H = high (4 mg)<br />
D = Diethylamine groups, weak anion exchanger M = medium (1 mg)<br />
Q = Quaternary ammonium groups, strong anion exchanger L = low (0.02 mg)<br />
S = Sulfonic acid groups, strong cation exchanger<br />
<strong>Catalogue</strong> No. Description Spin Columns Centrifuge Tubes Price<br />
Vivapure Mini (up to 0.5 ml)<br />
Order Code Description Spin Columns Price<br />
VS-IX01DH24 Vivapure D Mini H 24 £89.00<br />
VS-IX01DM24 Vivapure D Mini M 24 £60.00<br />
VS-IX01QH24 Vivapure Q Mini H 24 £89.00<br />
VS-IX01QM24 Vivapure Q Mini M 24 £60.00<br />
VS-IX01SH24 Vivapure S Mini H 24 £89.00<br />
VS-IX01SM24 Vivapure S Mini M 24 £60.00<br />
VS-IX01SQ16 Vivapure Mini H Starter Kit (8Q & 8S) 16 £120.00<br />
Vivapure Maxi (up to 20 ml) Protein binding capacity<br />
H = high (60 - 80 mg)<br />
M = medium (15 - 20 mg)<br />
Order Code Description Spin Columns Price<br />
VS-IX20DM08 Vivapure D Maxi M 8 £86.00<br />
VS-IX20DH08 Vivapure D Maxi H 8 £175.00<br />
VS-IX20QM08 Vivapure Q Maxi M 8 £86.00<br />
VS-IX20QH08 Vivapure Q Maxi H 8 £175.00<br />
VS-IX20SM08 Vivapure S Maxi M 8 £86.00<br />
VS-IX20SH08 Vivapure S Maxi H 8 £175.00<br />
Vivapure Anti HSA/IgG and Adenovirus/Lentivirus Purification Kits<br />
Order Code Description Spin Columns Price<br />
VS-SP08HAIGG Vivapure Anti-HSA/IgG Kit 12 £246.00<br />
VS-SP08HAR Vivapure Anti-HSA Kit 12 £155.00<br />
VS-SP50HAR Vivapure Anti-HSA Affinity Resin 50 ml £1,100.00<br />
VS-AVPQ020 Adenovirus Purification Kit (6 x 20 ml sample) £150.00<br />
VS-AVPQ101 Adenovirus Purification Kit (2 x 100 ml sample) £260.00<br />
VS-AVPQ501 Adenovirus Purification Kit (1 x 500 ml sample) £550.00
VC-LVPQ040 LentiSelect 40 Kit (4 x 40 ml sample) £290.00<br />
For purification of VSV-G pseudotyped lentivirus<br />
VC-LVPQ500 LentiSelect 500 Kit (1 x 500 ml sample) £500.00<br />
For purification of VSV-G pseudotyped lentivirus<br />
VC-LVPQ1000 LentiSelect 1000 Kit (1 x 1,000 ml sample) £850.00<br />
For purification of VSV-G pseudotyped lentivirus<br />
Vivapure Spin Columns and Kit Accessories<br />
Order Code Description Spin Columns Price<br />
VK01P042 Vivaclear Mini 0.8µm PES 100 £127.20<br />
VS-RP218L24 Vivapure C18 Micro spin columns 24 £50.00<br />
Vivawell Plates + Strips (IEX)<br />
Order Code Description Spin Columns Price<br />
VW96ID02 Vivawell 96 Well Plate D 2 £206.30<br />
VW96IS02 Vivawell 96 Well Plate S 2 £206.30<br />
VW96IQ02 Vivawell 96 Well Plate Q 2 £206.30<br />
VW08ID02 Vivawell 8 - Strip D 2 £291.50<br />
VW08IS02 Vivawell 8 - Strip S 2 £291.50<br />
VW08IQ02 Vivawell 8 - Strip Q 2 £291.50<br />
Vivascience Ultrafiltration Concentrators Price List<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Vivaspin 500 (100-600µl samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS0191 Vivaspin 500 Polyethersulfone 3,000 25 £51.30<br />
VS0192 Vivaspin 500 Polyethersulfone 3,000 100 £162.20<br />
VS0111 Vivaspin 500 Polyethersulfone 5,000 25 £51.30<br />
VS0112 Vivaspin 500 Polyethersulfone 5,000 100 £179.55<br />
VS0101 Vivaspin 500 Polyethersulfone 10,000 25 £49.80<br />
VS0102 Vivaspin 500 Polyethersulfone 10,000 100 £162.20<br />
VS0121 Vivaspin 500 Polyethersulfone 30,000 25 £51.30<br />
VS0122 Vivaspin 500 Polyethersulfone 30,000 100 £179.55<br />
VS0131 Vivaspin 500 Polyethersulfone 50,000 25 £51.30<br />
VS0132 Vivaspin 500 Polyethersulfone 50,000 100 £179.55<br />
VS0141 Vivaspin 500 Polyethersulfone 100,000 25 £51.30<br />
VS0142 Vivaspin 500 Polyethersulfone 100,000 100 £179.55<br />
VS0151 Vivaspin 500 Polyethersulfone 300,000 25 £51.30<br />
VS0152 Vivaspin 500 Polyethersulfone 300,000 100 £179.55<br />
VS0161 Vivaspin 500 Polyethersulfone 1,000,000 25 £51.30<br />
VS0162 Vivaspin 500 Polyethersulfone 1,000,000 100 £179.55<br />
VS0171 Vivaspin 500 Polyethersulfone 0.2 µm 25 £51.30<br />
VS0172 Vivaspin 500 Polyethersulfone 0.2 µm 100 £179.55<br />
VS01S1 Vivaspin 500 Polyethersulfone 25 £51.30<br />
Start Pack (5 of each 5K to 100K)
24 well Ultrafiltration Plate<br />
Order code Product Membrane MWCO Pack size Price<br />
VW24HT051 24-well Ultrafiltration Frames 2 £189.30<br />
(includes 2 collection plates)<br />
VW24PS0212 24-well filtrate collection plates 12 £14.40<br />
VS01HT01 Vivaspin 500 HT 10,000 480 £677.65<br />
(includes 120 cap strips)<br />
VS01HT21 Vivaspin 500 HT 30,000 480 £677.65<br />
(includes 120 cap strips)<br />
Vivaspin 2 (0.4 - 3.0 ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS0291 Vivaspin 2 Polyethersulfone 3,000 25 £61.00<br />
VS0292 Vivaspin 2 Polyethersulfone 3,000 100 £246.35<br />
VS0211 Vivaspin 2 Polyethersulfone 5,000 25 £80.30<br />
VS0212 Vivaspin 2 Polyethersulfone 5,000 100 £246.35<br />
VS0201 Vivaspin 2 Polyethersulfone 10,000 25 £61.00<br />
VS0202 Vivaspin 2 Polyethersulfone 10,000 100 £205.00<br />
VS0221 Vivaspin 2 Polyethersulfone 30,000 25 £80.30<br />
VS0222 Vivaspin 2 Polyethersulfone 30,000 100 £246.35<br />
VS0231 Vivaspin 2 Polyethersulfone 50,000 25 £80.30<br />
VS0232 Vivaspin 2 Polyethersulfone 50,000 100 £246.35<br />
VS0241 Vivaspin 2 Polyethersulfone 100,000 25 £80.30<br />
VS0242 Vivaspin 2 Polyethersulfone 100,000 100 £246.35<br />
VS0251 Vivaspin 2 Polyethersulfone 3,000,000 25 £80.30<br />
VS0252 Vivaspin 2 Polyethersulfone 3,000,000 100 £246.35<br />
VS0261 Vivaspin 2 Polyethersulfone 1,000,000 25 £80.30<br />
VS0262 Vivaspin 2 Polyethersulfone 1,000,000 100 £246.35<br />
VS0271 Vivaspin 2 Polyethersulfone 0.2 µm 25 £80.30<br />
VS0272 Vivaspin 2 Polyethersulfone 0.2 µm 100 £246.35<br />
VS02S1 Vivaspin 2 Polyethersulfone 25 £80.30<br />
Start Pack (5 of each 5K to 100K)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS02U1 Vivaspin 2 Cellulose Triacetate 5,000 25 £80.30<br />
VS02U2 Vivaspin 2 Cellulose Triacetate 5,000 100 £246.35<br />
VS02V1 Vivaspin 2 Cellulose Triacetate 10,000 25 £80.30<br />
VS02V2 Vivaspin 2 Cellulose Triacetate 10,000 100 £246.35<br />
VS02X1 Vivaspin 2 Cellulose Triacetate 20,000 25 £80.30<br />
VS02X2 Vivaspin 2 Cellulose Triacetate 20,000 100 £246.35<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Order code Product Membrane MWCO Pack size Price<br />
VS02H91 Vivaspin 2 Hydrosart® 2,000 25 £80.30<br />
VS02H92 Vivaspin 2 Hydrosart® 2,000 100 £246.35<br />
VS02H11 Vivaspin 2 Hydrosart® 5,000 25 £80.30<br />
VS02H12 Vivaspin 2 Hydrosart® 5,000 100 £246.35<br />
VS02H01 Vivaspin 2 Hydrosart® 10,000 25 £80.30<br />
VS02H02 Vivaspin 2 Hydrosart® 10,000 100 £246.35<br />
VS02H21 Vivaspin 2 Hydrosart® 30,000 25 £80.30<br />
VS02H22 Vivaspin 2 Hydrosart® 30,000 100 £246.35
Centrisart I-Centrifugal Units for rapid preparation of protein-free ultra-filtrates<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Order code Product Membrane MWCO Pack size Price<br />
13209-E Starter Kit Cellulose Triacetate Variety 12 £33.00<br />
13229-E Centrisart I Cellulose Triacetate 5,000 12 £33.00<br />
13239-E Centrisart I Cellulose Triacetate 10,000 12 £33.00<br />
13249-E Centrisart I Cellulose Triacetate 20,000 12 £33.00<br />
13269-E Centrisart I Polyethersulfone 100,000 12 £33.00<br />
13279-E Centrisart I Polyethersulfone 300,000 12 £33.00<br />
Vivaspin 4 (2 - 4ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS0413 Vivaspin 4 Polyethersulfone 5,000 25 £82.35<br />
VS0414 Vivaspin 4 Polyethersulfone 5,000 100 £272.70<br />
VS0403 Vivaspin 4 Polyethersulfone 10,000 25 £82.35<br />
VS0404 Vivaspin 4 Polyethersulfone 10,000 100 £272.70<br />
VS0423 Vivaspin 4 Polyethersulfone 30,000 25 £82.35<br />
VS0424 Vivaspin 4 Polyethersulfone 30,000 100 £272.70<br />
VS0433 Vivaspin 4 Polyethersulfone 50,000 25 £82.35<br />
VS0434 Vivaspin 4 Polyethersulfone 50,000 100 £272.70<br />
VS0443 Vivaspin 4 Polyethersulfone 100,000 25 £82.35<br />
VS0444 Vivaspin 4 Polyethersulfone 100,000 100 £272.70<br />
VS0473 Vivaspin 4 Polyethersulfone 0.2µm 25 £82.35<br />
VS0474 Vivaspin 4 Polyethersulfone 0.2µm 100 £272.70<br />
VS04S3 Vivaspin 4 Polyethersulfone varied 25 £82.35<br />
Vivaspin 6 (2 - 6 ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS0691 Vivaspin 6 Polyethersulfone 3,000 25 £107.30<br />
VS0692 Vivaspin 6 Polyethersulfone 3,000 100 £324.60<br />
VS0611 Vivaspin 6 Polyethersulfone 5,000 25 £76.85<br />
VS0612 Vivaspin 6 Polyethersulfone 5,000 100 £260.20<br />
VS0601 Vivaspin 6 Polyethersulfone 10,000 25 £76.85<br />
VS0602 Vivaspin 6 Polyethersulfone 10,000 100 £260.20<br />
VS0621 Vivaspin 6 Polyethersulfone 30,000 25 £107.30<br />
VS0622 Vivaspin 6 Polyethersulfone 30,000 100 £260.20<br />
VS0631 Vivaspin 6 Polyethersulfone 50,000 25 £107.30<br />
VS0632 Vivaspin 6 Polyethersulfone 50,000 100 £324.60<br />
VS0641 Vivaspin 6 Polyethersulfone 100,000 25 £107.30<br />
VS0642 Vivaspin 6 Polyethersulfone 100,000 100 £324.60<br />
VS0651 Vivaspin 6 Polyethersulfone 300,000 25 £107.30<br />
VS0652 Vivaspin 6 Polyethersulfone 300,000 100 £324.60<br />
VS0661 Vivaspin 6 Polyethersulfone 1,000,000 25 £107.30<br />
VS0662 Vivaspin 6 Polyethersulfone 1,000,000 100 £324.60<br />
VS0671 Vivaspin 6 Polyethersulfone 0.2 µm 25 £107.30<br />
VS0672 Vivaspin 6 Polyethersulfone 0.2 µm 100 £324.60<br />
VS06S1 Vivaspin 6 Polyethersulfone 25 £107.30<br />
Start Pack (5 of each 5K to 100K)
Vivaspin 15R (2 - 15ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS15RH91 Vivaspin 15R Hydrosart® 2,000 12 £74.90<br />
VS15RH92 Vivaspin 15R Hydrosart® 2,000 48 £240.90<br />
VS15RH11 Vivaspin 15R Hydrosart® 5,000 12 £74.90<br />
VS15RH12 Vivaspin 15R Hydrosart® 5,000 48 £240.90<br />
VS15RH01 Vivaspin 15R Hydrosart® 10,000 12 £74.90<br />
VS15RH02 Vivaspin 15R Hydrosart® 10,000 48 £240.90<br />
VS15RH21 Vivaspin 15R Hydrosart® 30,000 12 £74.90<br />
VS15RH22 Vivaspin 15R Hydrosart® 30,000 48 £240.90<br />
Vivaspin 15 (2 - 15ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS1511 Vivaspin 15 Polyethersulfone 5,000 10 £68.85<br />
VS1512 Vivaspin 15 Polyethersulfone 5,000 40 £232.20<br />
VS1501 Vivaspin 15 Polyethersulfone 10,000 10 £68.85<br />
VS1502 Vivaspin 15 Polyethersulfone 10,000 40 £232.20<br />
VS1521 Vivaspin 15 Polyethersulfone 30,000 10 £68.85<br />
VS1522 Vivaspin 15 Polyethersulfone 30,000 40 £232.20<br />
VS1531 Vivaspin 15 Polyethersulfone 50,000 10 £68.85<br />
VS1532 Vivaspin 15 Polyethersulfone 50,000 40 £232.20<br />
VS1541 Vivaspin 15 Polyethersulfone 100,000 10 £68.85<br />
VS1542 Vivaspin 15 Polyethersulfone 100,000 40 £232.20<br />
VS1571 Vivaspin 15 Polyethersulfone 0.2µm 10 £68.85<br />
VS1572 Vivaspin 15 Polyethersulfone 0.2µm 40 £232.20<br />
VS15S1 Vivaspin 15 Polyethersulfone 10 £68.85<br />
Start Pack (5 of each 5K to 100K)
Vivaspin 15 Accessories<br />
VSA001 Conical Bottom 50ml tubes and lids 100 £23.80<br />
Vivaspin 20 (2 - 20 ml samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS2091 Vivaspin 20 Polyethersulfone 3,000 12 £65.00<br />
VS2092 Vivaspin 20 Polyethersulfone 3,000 48 £198.75<br />
VS2011 Vivaspin 20 Polyethersulfone 5,000 12 £65.00<br />
VS2012 Vivaspin 20 Polyethersulfone 5,000 48 £198.75<br />
VS2001 Vivaspin 20 Polyethersulfone 10,000 12 £65.00<br />
VS2002 Vivaspin 20 Polyethersulfone 10,000 48 £198.75<br />
VS2021 Vivaspin 20 Polyethersulfone 30,000 12 £65.00<br />
VS2022 Vivaspin 20 Polyethersulfone 30,000 48 £198.75<br />
VS2031 Vivaspin 20 Polyethersulfone 50,000 12 £65.00<br />
VS2032 Vivaspin 20 Polyethersulfone 50,000 48 £198.75<br />
VS2041 Vivaspin 20 Polyethersulfone 100,000 12 £65.00<br />
VS2042 Vivaspin 20 Polyethersulfone 100,000 48 £198.75<br />
VS2051 Vivaspin 20 Polyethersulfone 300,000 12 £76.25<br />
VS2052 Vivaspin 20 Polyethersulfone 300,000 48 £230.00<br />
VS2061 Vivaspin 20 Polyethersulfone 1,000,000 12 £76.25<br />
VS2062 Vivaspin 20 Polyethersulfone 1,000,000 48 £230.00<br />
VS2071 Vivaspin 20 Polyethersulfone 0.2 µm 12 £76.25<br />
VS2072 Vivaspin 20 Polyethersulfone 0.2 µm 48 £230.00<br />
VS20S1 Vivaspin 20 Polyethersulfone 12 £76.25<br />
Start Pack (2 of each 5K to 0.2µm)
Vivaspin 20 Accessories<br />
VSA005 Diafiltration Cups 12 £15.00<br />
VCA200 Vivaspin 20 Pressure Head 1 £32.00<br />
VCA002 Air Pressure Controller (APC) 1 £185.50<br />
VCA005 Charge valve for Pressure-fuge head 1 £34.00<br />
VCA010 Female coupling 1 £26.00<br />
VCA011 Male coupling 1 £24.00<br />
VCA012 4 mm o.d. pressure tubing (3 m) 1 £6.50<br />
Vivacell 70 (10 - 70 ml Samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VS6011 Vivacell 70 Polyethersulfone 5,000 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6001 Vivacell 70 Polyethersulfone 10,000 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6021 Vivacell 70 Polyethersulfone 30,000 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6031 Vivacell 70 Polyethersulfone 50,000 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6041 Vivacell 70 Polyethersulfone 100,000 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6071 Vivacell 70 Polyethersulfone 0.2 µm 2 £33.10<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6012 Vivacell 70 Polyethersulfone 5,000 10 £98.00<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6002 Vivacell 70 Polyethersulfone 10,000 10 £137.70<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6022 Vivacell 70 Polyethersulfone 30,000 10 £137.70<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6032 Vivacell 70 Polyethersulfone 50,000 10 £137.70<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6042 Vivacell 70 Polyethersulfone 100,000 10 £137.70<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
VS6072 Vivacell 70 Polyethersulfone 0.2 µm 10 £137.70<br />
(Includes 2 x 250 ml Centrifuge Bottles)<br />
Vivacell 70 Accessories<br />
Order code Product Pack size Price<br />
VCA002 Air Pressure Controller (APC) 1 £185.50<br />
VSA003 250 ml Centrifuge bottle 4 £55.00<br />
VCA004 250 ml Centrifuge bottle with modified cap 2 £35.00<br />
VCA005 Charge valve for Pressure-fuge head 1 £34.00<br />
VCA007 Replacement seals for Pressure-fuge head 10 £13.00<br />
VCA010 Female coupling 1 £26.00<br />
VCA011 Male coupling 1 £24.00<br />
VCA012 4 mm o.d. pressure tubing (3 m) 1 £6.50<br />
VCA700 Pressure head with reservoir & Filtrate Bottle 1 £115.00<br />
VCA701 Pressure-fuge head 2 £135.00
Vivacell 100 (10 - 100ml Samples)<br />
Order code Product Membrane MWCO Pack size Price<br />
VC1011 Vivacell 100 Polyethersulfone 5,000 2 £42.50<br />
VC1012 Vivacell 100 Polyethersulfone 5,000 10 £192.00<br />
VC1001 Vivacell 100 Polyethersulfone 10,000 2 £42.50<br />
VC1002 Vivacell 100 Polyethersulfone 10,000 10 £192.00<br />
VC1021 Vivacell 100 Polyethersulfone 30,000 2 £42.50<br />
VC1022 Vivacell 100 Polyethersulfone 30,000 10 £192.00<br />
VC1031 Vivacell 100 Polyethersulfone 50,000 2 £42.50<br />
VC1032 Vivacell 100 Polyethersulfone 50,000 10 £192.00<br />
VC1041 Vivacell 100 Polyethersulfone 100,000 2 £42.50<br />
VC1042 Vivacell 100 Polyethersulfone 100,000 10 £192.00<br />
VC1051 Vivacell 100 Polyethersulfone 300,000 2 £42.50<br />
VC1052 Vivacell 100 Polyethersulfone 300,000 10 £192.00<br />
VC1061 Vivacell 100 Polyethersulfone 1,000,000 2 £42.50<br />
VC1062 Vivacell 100 Polyethersulfone 1,000,000 10 £192.00<br />
VC1071 Vivacell 100 Polyethersulfone 0.2 µm 2 £42.50<br />
VC1072 Vivacell 100 Polyethersulfone 0.2 µm 10 £192.00<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Vivacell 100 Accessories<br />
Order code Product Pack size Price<br />
VCA800 Vivacell 100 Pressure Head with reservoir 1 £86.00<br />
VCA013 Concentrator Cap 2 £89.00<br />
VCA014 Santoprene Replacement Seals 10 £9.90<br />
VCA002 Air Pressure Controller (APC) 1 £185.50<br />
VCA010 Female coupling 1 £26.00<br />
VCA011 Male coupling 1 £24.00<br />
VCA012 4 mm o.d. pressure tubing (3 m) 1 £6.50<br />
BJPA-P500 Plastic recovery pipettes 500 £42.00<br />
Vivacell 250<br />
Order code Product Membrane MWCO Pack size Price<br />
VCA250 Vivacell 250 complete with accessories 1 £225.00<br />
VC2501 Vivacell 250 Polyethersulfone 10,000 5 £74.00<br />
VC2511 Vivacell 250 Polyethersulfone 5,000 5 £74.00<br />
VC2521 Vivacell 250 Polyethersulfone 30,000 5 £74.00<br />
VC2541 Vivacell 250 Polyethersulfone 100,000 5 £74.00<br />
VC2571 Vivacell 250 Polyethersulfone 0.2 µm 5 £74.00<br />
VC25S1 Vivacell 250 Polyethersulfone 5 £74.00<br />
Start Pack (1 of each 5K to 100K)
Vivacell 250 Accessories<br />
Order code Product Pack size Price<br />
VCA002 Air Pressure Controller (APC) 1 £185.50<br />
VCA008 Pressure indicator/relief valve 1 £35.00<br />
VCA009 Vivacell 250 Maintenance Kit 1 £39.00<br />
VCA010 Female coupling 1 £26.00<br />
VCA011 Male coupling 1 £24.00<br />
VCA012 4 mm o.d. pressure tubing (3 m) 1 £6.50<br />
VCA015 Vivacell 250 pressure head 1 £108.00<br />
Membrane MWCO Pack Size Price<br />
Vivaflow 50 Tangential Flow Products<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Order code Product Membrane MWCO Pack size Price<br />
VF05P9 Vivaflow 50 Polyethersulfone 3,000 2 £78.30<br />
VF05P1 Vivaflow 50 Polyethersulfone 5,000 2 £78.30<br />
VF05P0 Vivaflow 50 Polyethersulfone 10,000 2 £78.30<br />
VF05P2 Vivaflow 50 Polyethersulfone 30,000 2 £78.30<br />
VF05P3 Vivaflow 50 Polyethersulfone 50,000 2 £78.30<br />
VF05P4 Vivaflow 50 Polyethersulfone 100,000 2 £78.30<br />
VF05P7 Vivaflow 50 Polyethersulfone 0.2 µm 2 £78.30<br />
VF05C0 Vivaflow 50 Regen Cellulose 10,000 2 £78.30<br />
VF05C2 Vivaflow 50 Regen Cellulose 30,000 2 £78.30<br />
VF05C4 Vivaflow 50 Regen Cellulose 100,000 2 £78.30<br />
Vivaflow 50 Accessories<br />
Order code Product Pack size Price<br />
VFP001 Masterflex Economy Drive Peristaltic Pump 230V 1 £520.00<br />
VFA006 500 ml Sample/Diafiltration Reservoir 1 £70.00<br />
VFA010 Masterflex Standard Pump Head - Size 16 1 £165.00<br />
VFA012 Masterflex Easy Load Pump Head - Size 16 1 £175.00<br />
VFA016 Vivaflow 50 Stand (one or two stack position) 1 £80.50<br />
VFA020 Pressure Indicator (0.5-3 bar) 1 £16.00<br />
Vivaflow Tubing and Fittings<br />
VFA004 Tygon Size 16 pump tubing & luer fittings 3 meters £18.00<br />
VFA009 Flow restrictors (set of 2 x 0.4, 0.6, 0.8mm) 1 £6.40<br />
VFA030 T Connectors for running 2 stacks 2 £5.90<br />
VFA031 Series Interconnectors 6 £12.00<br />
VFA032 Female Luer fittings 10 £5.25<br />
VFA034 Tubing set for Vivaflow 50 1 £39.00<br />
VFA035 Flow restrictors 6 X 0.6 pink 6 £5.65<br />
Vivaflow 50 Complete System (Ready to Run) comprises<br />
VFS502 Pump, Easy Load Pump Head (size 16), tubing, 1 £691.00<br />
500 ml diafiltration reservoir, module stand,<br />
pressure indicator, T connectors, interconnectors
Vivaflow 200 Tangential Flow Products<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Order code Product Membrane MWCO Pack size Price<br />
VF20P1 Vivaflow 200 Polyethersulfone 5,000 1 £199.80<br />
VF20P0 Vivaflow 200 Polyethersulfone 10,000 1 £199.80<br />
VF20P2 Vivaflow 200 Polyethersulfone 30,000 1 £199.80<br />
VF20P3 Vivaflow 200 Polyethersulfone 50,000 1 £199.80<br />
VF20P4 Vivaflow 200 Polyethersulfone 100,000 1 £199.80<br />
VF20P7 Vivaflow 200 Polyethersulfone 0.2 µm 1 £199.80<br />
VF20C0 Vivaflow 200 Regen Cellulose 10,000 1 £199.80<br />
VF20C2 Vivaflow 200 Regen Cellulose 30,000 1 £199.80<br />
VF20C4 Vivaflow 200 Regen Cellulose 100,000 1 £199.80<br />
VF20H0 Vivaflow 200 Hydrosart® 10,000 1 £199.80<br />
VF20H1 Vivaflow 200 Hydrosart® 5,000 1 £199.80<br />
VF20H2 Vivaflow 200 Hydrosart® 30.000 1 £199.80<br />
Hydrosart is a cellulose-based membrane from Sartorius-Stedim Biotech.<br />
Vivaflow 200 Accessories<br />
Order code Product Pack size Price<br />
VFP001 Masterflex Economy Drive Peristaltic Pump 230V 1 £520.00<br />
VFA001 Vivaflow Recirculation Assembly 1 £70.00<br />
VFA006 500 ml Sample/Diafiltration Reservoir 1 £70.00<br />
VFA010 Masterflex Standard Pump Head - Size 16 1 £165.00<br />
VFA011 Masterflex Standard Pump Head - Size 15 1 £165.00<br />
VFA012 Masterflex Easy Load Pump Head - Size 16 1 £175.00<br />
VFA013 Masterflex Easy Load Pump Head - Size 15 1 £180.00
Vivaflow 200 Tubing and Fittings<br />
Order code Product Pack size Price<br />
VFA003 Tygon Size 15 pump tubing & luer fittings 3 meters £28.00<br />
Order code Product Pack size Price<br />
VFA004 Tygon Size 16 pump tubing & luer fittings 3 meters £18.00<br />
VFA005 Y Connector (size 15 to 2 x size 16) 1 £5.00<br />
VFA009 Flow restrictors (set of 2 x 0.4, 0.6, 0.8mm) 1 £6.40<br />
VFA032 Female luer fittings size 16 10 £5.25<br />
VFA035 Flow Restrictor (0.6mm) 6 £5.65<br />
VFA036 Female luer fittings size 15 10 £5.00<br />
<strong>Catalogue</strong> No. Description Membrane MWCO Pack Size Price<br />
Vivaflow 200 Complete System (Ready to Run) comprises<br />
VFS202 Pump, Easy Load Pump Head (size 16), 1 £592.00<br />
tubing, 500 ml sample/diafiltration Reservoir<br />
Classic Minisart Syringe Filters from Sartorius<br />
Order code Product Pack size Price<br />
16534 K 0.2 µm Minisarts, sterile, individually-packed, 50 £27.00<br />
with luer lock outlet.<br />
16555 K 0.45 µm Minisarts, sterile, individually-packed, 50 £27.00<br />
with luer lock outlet.<br />
Proteus Mini Clarification Spin Columns from <strong>Generon</strong><br />
Order code Product Pack size Price<br />
GEN-MSF500 Mini Clarification Spin Column Pack 100 £78.00<br />
(0.2 µm low protein-binding hydrophilic PVDF)
<strong>Generon</strong>’s Chromatography Resins, Columns and Kits<br />
Proteus Affinity Resin from <strong>Generon</strong><br />
PC-MC25 Ni 2+ -IDA Metal Chelate Sepharose® resin (25 ml) £125.00<br />
PC-MC100 Ni 2+ -IDA Metal Chelate Sepharose® resin (100 ml) £380.00<br />
GEN-MCS25 Ni-Superflow® Plus resin (25 ml; NiNTA Equiv.) £182.00<br />
GEN-MCS100 Ni-Superflow® Plus resin (100 ml; NiNTA Equiv.) £665.00<br />
GEN-MCS500 Ni-Superflow® Plus resin (500 ml; NiNTA Equiv.) £2,450.00<br />
PC-A1 Recombinant Protein A Sepharose® FF resin (1 ml) £70.00<br />
PC-A5 Recombinant Protein A Sepharose® FF resin (5 ml) £210.00<br />
PC-A25 Recombinant Protein A Sepharose® FF resin (25 ml) £500.00<br />
PC-A100 Recombinant Protein A Sepharose® FF resin (100 ml) £1,490.00<br />
PC-G1 Recombinant Protein G Sepharose® FF resin (1 ml) £70.00<br />
PC-G5 Recombinant Protein G Sepharose® FF resin (5 ml) £210.00<br />
PC-G25 Recombinant Protein G Sepharose® FF resin (25 ml) £500.00<br />
PC-G100 Recombinant Protein G Sepharose® FF resin (100 ml) £1,490.00<br />
SuperGlu10 Glutathione Superflow® (10 ml) £120.00<br />
SuperGlu25 Glutathione Superflow® (25 ml) £275.00<br />
SuperGlu100 Glutathione Superflow® (100 ml) £900.00<br />
SuperGlu500 Glutathione Superflow® (500 ml) £4,100.00<br />
Proteus 1-Step Back Midi Spin Columns from <strong>Generon</strong><br />
Order code Product Pack size Price<br />
GEN-1SB08 1-Step Batch Spin Column Pack 8 £32.00<br />
Proteus Mini Purification Columns from <strong>Generon</strong><br />
GEN-MPS500 Mini Purification Spin Column Pack 25 £25.00<br />
Single StEP Empty Batch/Pump Elution Columns from Thompson<br />
9452086-10 10 ml Single StEP column with bottom frit 10 £98.00<br />
9452088-10 25 ml Single StEP column with bottom frit 10 £98.00<br />
9452090-10 50 ml Single StEP column with bottom frit 10 £108.00<br />
9452092-10 100ml Single StEP column with bottom frit 10 £148.00<br />
9452094-10 200 ml Single StEP column with bottom frit 10 £148.00<br />
9452099-10 320 ml Single StEP column with bottom frit 10 £310.00<br />
9452097-10 650 ml Single StEP column with bottom frit 10 £355.00<br />
235008-20 Dual ended Caps (Blue) 20 £25.00
Proteus FliQ Columns for AKTAs<br />
Order code Description Pack Size Prices<br />
GEN-FliQ1 Proteus 1 ml FliQ column 1 £15.00<br />
GEN-FliQ5 Proteus 5 ml FliQ column 1 £24.00<br />
GEN-FliQ10 Proteus 10 ml FliQ column 1 £35.00<br />
GEN-FliQ20 Proteus 20 ml FliQ column 1 £70.00<br />
GEN-10.32 10.32 packing connector 1 £6.50<br />
Protein Desalting Columns from emp Biotech GmbH (P25 = PD-10 equivalents)<br />
Order code Description Pack Size Prices<br />
GEN-CP-0201-25 Centripure Mini Columns 25 pc £53.00<br />
GEN-CP-0201-100 Centripure Mini Columns 100 pc £187.50<br />
GEN-CP-0110-50 Centripure P2 Columns 50 pc £136.00<br />
GEN-CP-0106-50 Centripure P5 Columns 50 pc £175.00<br />
GEN-CP-0107-50 Centripure P10 Columns 50 pc £187.50<br />
GEN-CP-0108-25 Centripure P25 Columns 25 pc £125.00<br />
GEN-CP-0113-10 Centripure P50 Columns 10 pc £210.00<br />
GEN-CP-0106-50 Centripure P5 Columns 50 pc £175.00<br />
Proteus Protein A and G Spin Column Kits<br />
Order code Description Contents Price<br />
Protein A & G Mini Starter Pack<br />
PC-MAG04 Protein A & G Starter Pack Includes UF concentrators £59.00<br />
-2 x Mini A and 2 x Mini G and buffers<br />
plugs<br />
Order code Description Contents Price<br />
Protein A Mini<br />
PC-MA02 Protein A Mini Sample kit Incl. UF concentrators £60.00<br />
-2 x Mini A plugs and buffers<br />
PC-MAK16 Protein A Mini kit Incl. UF concentrators £266.00<br />
-16 x Mini A plugs and buffers<br />
PC-MAC48 Protein A Mini Bulk Pack No UF concentrators £433.00<br />
-48 x Mini A plugs and buffers<br />
Order code Description Contents Price<br />
Protein A Midi<br />
PC-AK04 Protein A Midi kit Incl. UF concentrators £246.00<br />
-4 x Midi A plugs and buffers<br />
PC-AC12 Protein A Midi Bulk Pack No UF concentrators £422.00<br />
-12 x Midi A plugs and buffers
Protein G Mini<br />
PC-MG02 Protein G Mini Sample kit Incl. UF concentrators £63.00<br />
-2 x Mini G plugs and buffers<br />
PC-MGK16 Protein G Mini kit Incl. UF concentrators £287.00<br />
-16 x Mini G plugs and buffers<br />
PC-MGC48 Protein G Mini Bulk Pack No UF concentrators £486.00<br />
-48 x Mini G plugs and buffers<br />
Protein G Midi<br />
PC-GK04 Protein G Midi kit Incl. UF concentrators £266.00<br />
-4 x Midi G plugs and buffers<br />
PC-GC12 Protein G Midi Bulk Pack No UF concentrators £565.00<br />
-12 x Midi G plugs and buffers<br />
Proteus IMAC Spin Column Kits<br />
Order code Description Contents Price<br />
IMAC Mini<br />
PC-MMCK04 MC Mini Sample kit Incl. UF concentrators £63.00<br />
-4 x Mini MC plugs and buffers<br />
PC-MMCK24 MC Mini kit Incl. UF concentrators £227.00<br />
-24 x Mini MC plugs and buffers<br />
PC-MMCC24 MC Mini Pack Incl. buffers £180.00<br />
-24 x Mini MC plugs<br />
PC-MMCC72 MC Mini Bulk Pack No UF concentrators £400.00<br />
-72 x Mini MC plugs and buffers<br />
Order code Description Contents Price<br />
IMAC Midi<br />
PC-MCK08 MC Midi kit Incl. UF concentrators £200.00<br />
-8 x MC plugs and buffers<br />
PC-MCC08 MC Midi Pack Incl. buffers £167.00<br />
-8 x MC plugs<br />
PC-MCC24 MC Midi Bulk Pack No UF concentrators £399.00<br />
-24 x MC plugs and buffers<br />
Accessories<br />
PC-BAP Protein A Buffer Pack Buffers only £35.00<br />
PC-BGP Protein G Buffer Pack Buffers only £33.00<br />
PC-BMCP Metal Chelate Buffer Pack Buffers only £33.00<br />
PC-PPCSC Peristaltic Pump Collar for Proteus Spin Columns £75.00
Proteus NoEndo Spin Column Kits<br />
Order code Description Contents Price<br />
Protein NoEndo Starter Pack<br />
GEN-NoE2M Proteus NoEndoM 2 x 0.25 ml NoEndo £60.00<br />
Starter Pack Mini resin vials<br />
GEN-NoE2S Proteus NoEndoS 2 spin columns £70.00<br />
Starter Pack<br />
GEN-NoE2HC Proteus NoEndoHC 2 HC spin columns £80.00<br />
Starter Pack<br />
Proteus NoEndo Mini<br />
GEN-NoE12M Proteus NoEndoM Kit 12 x 0.25 ml NoEndo £220.00<br />
Mini resin vials<br />
GEN-NoE48M Proteus NoEndoM 48 x 0.25 ml NoEndo £720.00<br />
Bulk Pack Mini resin vials<br />
Proteus NoEndo Standard<br />
GEN-NoE12S Proteus NoEndoS Kit 12 S spin columns £280.00<br />
GEN-NoE48S Proteus NoEndoS 48 S spin columns £930.00<br />
Bulk Pack<br />
Proteus NoEndo High Capacity<br />
GEN-NoE12HC Proteus NoEndoHC Kit 12 HC spin columns £330.00<br />
GEN-NoE48HC Proteus NoEndoHC 48 HC spin columns £1,060.00<br />
Bulk Pack<br />
Accessories<br />
PC-PPCSC Peristaltic Pump Collar for Proteus Spin Columns £75.00<br />
GEN-NoE025ML NoEndo Resin (25 ml) PoA<br />
GEN-NoE0100ML NoEndo Resin (100 ml) PoA
Pro-Chem BJP Static Concentrator Price List<br />
Order code Description Price<br />
BJP-5/40 40 tests - Pack of five 8-test-block units, £140.00<br />
5 ml sample volume, 7,500 MWCO<br />
(excludes transfer pipettes)<br />
BJP-10/40 40 tests - Pack of five 8-test-block units, £140.00<br />
10 ml sample volume, 7,500 MWCO<br />
(excludes transfer pipettes)<br />
BJP-5/30 30 individual units, 5 ml sample volume, 7,500 MWCO £115.00<br />
(excludes transfer pipettes)<br />
BJP-10/30 30 individual units, 10 ml sample volume, 7,500 MWCO £115.00<br />
(excludes transfer pipettes)<br />
BJP-20/30 30 individual units, 20ml sample volume, 7,500 MWCO £120.00<br />
(excludes transfer pipettes)<br />
BJP-5/100 100 individual units, 5 ml sample volume, 7,500 MWCO £265.00<br />
(excludes transfer pipettes)<br />
BJP-10/100 100 individual units, 10 ml sample volume, 7,500 MWCO £265.00<br />
(excludes transfer pipettes)<br />
BJP-20/100 100 individual units, 20 ml sample volume, 7,500 MWCO £285.00<br />
(excludes transfer pipettes)<br />
BJP Accessories<br />
BJPA-P500 Pack of 500 plastic transfer pipettes £42.00<br />
for use with BJP units<br />
BJPA-ER20 Pack of 10 expansion reservoirs for use £20.00<br />
with BJP 20 devices<br />
BJPA-AS Acrylic stand for BJP individual units FoC
Owl DNA/RNA Electrophoresis Price list<br />
Horizontal Mini Electrophoresis System<br />
Order code Description Price<br />
B1A (EasyCast) Minigel System, 7 x 8 cm (B1A) £250.00<br />
B1A-10 10 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1A-12 12 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1A-5C 5 tooth comb, 1.0 mm thick £32.50<br />
B1A-5D 5 tooth comb, 1.5 mm thick £32.50<br />
B1A-6 6 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1A-8 8 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1A-BP Minigel System with BEP, 7 x 8 cm £284.00<br />
B1A-CST Casting chamber £49.70<br />
B1A-GK Replacement Gasket Set of 2 £10.00<br />
B1A-LID Lid for B1A Unit with Attached Power Supply Leads £41.50<br />
B1A-PREP Prep/Marker Comb, 1.5mm thick £32.50<br />
B1A-UVT Gasketed UVT gel tray £49.90<br />
B1 (EasyCast) Minigel System, 9 x 11 cm (B1) £285.00<br />
B1-10 10 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1-12C 12 tooth comb, 1.0 mm thick £32.50<br />
B1-12D 12 tooth comb, 1.5 mm thick £32.50<br />
B1-14 14 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B1-5C 5 tooth comb, 1.0 mm thick £32.50<br />
B1-5D 5 tooth comb, 1.5 mm thick £32.50
B1-8C 8 tooth comb, 1.0 mm thick £32.50<br />
B1-8D 8 tooth comb, 1.5 mm thick £32.50<br />
B1-BP Minigel System with BEP, 9 x 11 cm £320.00<br />
B1-CST Casting chamber £58.00<br />
B1-GK Replacement Gasket Set of 2 £10.00<br />
B1-LID Lid for B1 Unit with Attached Power Supply Leads £49.80<br />
B1-PREP Prep/Marker Comb, 1.5mm thick £32.50<br />
B1-RL-17C B1 rapid load 17 well comb, 1.0mm £32.50<br />
B1-RL-17D B1 rapid load 17 well comb, 1.5mm £32.50<br />
B1-RL-9C B1 rapid load 9 well comb, 1.0mm £32.50<br />
B1-RL-9D B1 rapid load 9 well comb, 1.5mm £32.50<br />
B1-UVT Gasketed UVT gel tray £58.10<br />
B2 (EasyCast) Minigel System, 12 x 14 cm (B2) £318.00<br />
B2-12 12 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B2-16 16 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B2-20 20 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B2-24 24 tooth comb, 1.0/1.5 mm thick Double Sided £32.50<br />
B2-8C 8 tooth comb, 1.0 mm thick £32.50<br />
B2-8D 8 tooth comb, 1.5 mm thick £32.50<br />
B2-BP Minigel System with BEP, 12 x 14 cm £345.00<br />
B2-CST Casting chamber £63.00<br />
B2-GK Replacement Gasket Set of 2 £10.00<br />
B2-LID Lid for B2 Unit with Attached Power Supply Leads £53.10<br />
B2-PREP Preparative comb, 1.5 mm thick £32.50<br />
B2-RL B2/B3 Multi Load System £310.00
B2-RL-25C B2 Multi Load 25 well comb, 1.0mm £24.00<br />
B2-RL-25D B2 Multi Load 25 well comb, 1.5mm £24.00<br />
B2-RL-9C B2 Multi Load 9 well comb, 1.0mm £24.00<br />
B2-RL-9D B2 Multi Load 9 well comb, 1.5mm £24.00<br />
B2-RL-UVT Multi Load UVT gel tray for B2 system £76.00<br />
B2-UVT Gasketed UVT gel tray £65.00<br />
B2-WALL Wall comb. 1.5 mm thick £32.50<br />
Power<br />
Supplies<br />
OSP300-2Q NEW! Power Supply (O-300 V, 400 mA, 75 W, 3 jacks) £560.00<br />
OSP200-2Q NEW! Power Supply (O-200 V, 2A, 200 W, 4 jacks) £1,350.00<br />
OSP1000-2Q NEW! Power Supply (O-1000 V, 500 mA, 250 W, 4 jacks) £1,480.00<br />
Cell Culture<br />
GEN-00-025 alamarBlue Stain (25 ml) £100.00<br />
GEN-00-100 alamarBlue Stain (100 ml) £209.00<br />
<strong>Generon</strong> Ltd., 12 Rawcliffe House, Howarth Rd, Maidenhead, Berkshire SL6 1AP.<br />
Order and Technical Support Tel: (01753) 866-511<br />
Order Fax: (01753) 20-88-99<br />
Order E-mail: info@generon.co.uk<br />
WWW: www.generon.co.uk<br />
*All prices exclude VAT and are FOB <strong>Generon</strong> UK
CELLine 2-compartment Bioreactor<br />
Cell Culture<br />
Order code Description Price<br />
DC-90010 CELLine 350 (Disposable two–compartment £380.00<br />
Bioreactor for suspension cell cultures, 350 ml<br />
media volume, 5 ml culture volume, 5 pc)<br />
DC-90005 CELLine 1000 (Disposable two – compartment £249.00<br />
Bioreactor for suspension cell cultures, 1000 ml<br />
media volume, 15 ml culture volume, 3 pc)<br />
Cultiflask 50<br />
Order code Description Price<br />
DF-050MB-SSH---4 Cultiflask 50 disposable bioreactor, 180 pc £54.00<br />
UltraYield Flasks from Thomson<br />
Order code Description Price<br />
Sterile Flasks<br />
931136-B 2.5 L Ultra Yield Flasks; 500 ml-1 L media (6 pc) £94.95<br />
931141 500 ml Ultra Yield Flasks, 125-200 ml media (25 pc) £244.95<br />
931144 250 ml Ultra Yield Flasks, 75-110 ml media (50 pc) £279.95<br />
931147 125 ml Ultra Yield Flasks, 40-50 ml media (50 pc) £249.95<br />
Sterile AirOTop Seals<br />
899425 AirOTop enhanced seals for 2.5L Flasks (100 pc) £115.00<br />
899415 AirOTop seals for 125, 250 and 500 ml flasks (100 pc) £69.95<br />
Cell Culture Plates from Thomson<br />
Culture Plates<br />
931565-G-1X 24 well plate (10.4 ml PP, £99.00<br />
individually-wrapped sterile (20 pc)<br />
931130 96 well plate (2 ml PP, £96.00<br />
individually-wrapped sterile (32 pc)<br />
Sterile Airoporous Seals<br />
899410 Rectangular seals for Growing Cultures (100 pc) £65.00<br />
Adhesive Foil Seals<br />
899405-1 Adhesive Foil Seal (100 pc) £65.00
Alvetex 3-D Cell Culture<br />
Order code Description Price<br />
AVP002 12 well Alvetex ® plate (1 pc) £78.00<br />
AVP015 Alvetex ® well insert holder and petri-dish (1 pc) £26.00<br />
AVP-004-3 Alvetex ® 6 well insert (3 pc) £39.00<br />
AVP-005-3 Alvetex ® 12 well insert (3 pc) £33.00<br />
Complete Filter Units<br />
Nalgene Disposable Vacuum filtration Units<br />
Order code Description Price<br />
568-0020 Nalgene Sterile Mach V Supor Filter Unit, PES, £56.00<br />
0.2 µm pore size (250 ml; 12 pc)<br />
566-0020 Nalgene Sterile Mach V Supor Filter Unit, PES, £74.00<br />
0.2 µm pore size (500 ml; 12 pc)<br />
567-0020 Nalgene Sterile Mach V Supor Filter Unit, PES, £93.00<br />
0.2 µm pore size (1,000 ml; 12 pc)<br />
Bottle-Top filters (excl. receiver flasks)<br />
Order code Description Price<br />
595-4520 Nalgene Sterile Mach V Supor B-Top Filter, PES, £49.00<br />
0.2 µm pore size (500 ml; 12 pc)<br />
597-4520 Nalgene Sterile Mach V Supor B-Top Filter, PES, £89.00<br />
0.2 µm pore size (1,000 ml; 12 pc)<br />
Filter Plates from Thomson<br />
Order code Description Price<br />
931919 96 well filter plate, 2 ml, 25 µm sinter (25 pc) £420.00<br />
982080 96 well filter plate, 800 µl, 25 µm sinter, Long drip (25 pc) £395.00<br />
Pierce Coomassie Plus Bradford Reagent Kit<br />
Order code Description Price<br />
GEN-23236 Pierce Coomassie Plus Bradford Reagent (950 ml) £175.00<br />
Albumin Standard Ampules, 2mg/mL, 10 x 1mL<br />
Sufficient for 630 tube assays or 3,160 microplate assays
10 well<br />
Nusep Pre-cast Tris-HEPES UNIVERSAL Protein Gels<br />
For Bio-Rad, Invitrogen, Hoefer and Owl Tanks<br />
Order code Description Price<br />
NH21-008 Protein Gels, 10 wells x 50µl ea, 8 %, 10 gels £59.95<br />
NH21-010 Protein Gels, 10 wells x 50µl ea, 10 %, 10 gels £59.95<br />
NH21-012 Protein Gels, 10 wells x 50µl ea, 12 %, 10 gels £59.95<br />
NH21-420 Protein Gels, 10 wells x 50µl ea, 4-20 %, 10 gels £59.95<br />
NH21-816 Protein Gels, 10 wells x 50µl ea, 8-16 %, 10 gels £59.95<br />
12 well<br />
Order code Description Price<br />
NH11-008 Protein Gels, 12 wells x 30µl ea, 8 %, 10 gels £59.95<br />
NH11-010 Protein Gels, 12 wells x 30µl ea, 10 %, 10 gels £59.95<br />
NH11-012 Protein Gels, 12 wells x 30µl ea, 12 %, 10 gels £59.95<br />
NH11-420 Protein Gels, 12 wells x 30µl ea, 4-20 %, 10 gels £59.95<br />
NH11-816 Protein Gels, 12 wells x 30µl ea, 8-16 %, 10 gels £59.95<br />
15 well<br />
Order code Description Price<br />
NH31-008 Protein Gels, 15 wells x 25µl ea, 8 %, 10 gels £59.95<br />
NH31-010 Protein Gels, 15 wells x 25µl ea, 10 %, 10 gels £59.95<br />
NH31-012 Protein Gels, 15 wells x 25µl ea, 12 %, 10 gels £59.95<br />
NH31-420 Protein Gels, 15 wells x 25µl ea, 4-20 %, 10 gels £59.95<br />
NH31-816 Protein Gels, 15 wells x 25µl ea, 8-16 %, 10 gels £59.95
10 well<br />
Nusep Pre-cast Tris-Glycine UNIVERSAL Protein Gels<br />
For Bio-Rad, Invitrogen, Hoefer and Owl Tanks<br />
Order code Description Price<br />
NG21-008 Protein Gels, 10 wells x 50µl ea, 8 %, 10 gels £59.95<br />
NG21-010 Protein Gels, 10 wells x 50µl ea, 10 %, 10 gels £59.95<br />
NG21-012 Protein Gels, 10 wells x 50µl ea, 12 %, 10 gels £59.95<br />
NG21-420 Protein Gels, 10 wells x 50µl ea, 4-20 %, 10 gels £59.95<br />
NG21-816 Protein Gels, 10 wells x 50µl ea, 8-16 %, 10 gels £59.95<br />
12 well<br />
Order code Description Price<br />
NG11-010 Protein Gels, 12 wells x 30µl ea, 10 %, 10 gels £59.95<br />
NG11-012 Protein Gels, 12 wells x 30µl ea, 12 %, 10 gels £59.95<br />
NG11-420 Protein Gels, 12 wells x 30µl ea, 4-20 %, 10 gels £59.95<br />
NG11-816 Protein Gels, 12 wells x 50µl ea, 8-16 %, 10 gels £59.95<br />
15 well<br />
Order code Description Price<br />
NG31-010 Protein Gels, 15 wells x 25µl ea, 10 %, 10 gels £59.95<br />
NG31-012 Protein Gels, 15 wells x 25µl ea, 12 %, 10 gels £59.95<br />
NG31-420 Protein Gels, 15 wells x 25µl ea, 4-20 %, 10 gels £59.95<br />
NG31-816 Protein Gels, 15 wells x 25µl ea, 8-16 %, 10 gels £59.95
10 well<br />
Nusep Pre-cast Tris-Glycine MAX Protein Gels<br />
For Invitrogen Tanks Only<br />
Order code Description Price<br />
NN10-008 Protein Max Gels, 10 wells x 50µl ea, 8 %, 10 gels £59.95<br />
NN10-010 Protein Max Gels, 10 wells x 50µl ea, 10 %, 10 gels £59.95<br />
NN10-012 Protein Max Gels, 10 wells x 50µl ea, 12 %, 10 gels £59.95<br />
NN10-420 Protein Max Gels, 10 wells x 50µl ea, 4-20 %, 10 gels £59.95<br />
NN10-816 Protein Max Gels, 10 wells x 50µl ea, 8-16 %, 10 gels £59.95<br />
12 well<br />
Order code Description Price<br />
NN12-010 Protein Max Gels, 12 wells x 30µl ea, 10 %, 10 gels £59.95<br />
NN12-012 Protein Max Gels, 12 wells x 30µl ea, 12 %, 10 gels £59.95<br />
NN12-420 Protein Max Gels, 12 wells x 30µl ea, 4-20 %, 10 gels £59.95<br />
NN12-816 Protein Max Gels, 12 wells x 30µl ea, 8-16 %, 10 gels £59.95<br />
17 well<br />
Order code Description Price<br />
NN17-010 Protein Max Gels, 17 wells x 30µl ea, 10 %, 10 gels £59.95<br />
NN17-012 Protein Max Gels, 17 wells x 30µl ea, 12 %, 10 gels £59.95<br />
NN17-420 Protein Max Gels, 17 wells x 30µl ea, 4-20 %, 10 gels £59.95<br />
NN17-816 Protein Max Gels, 17 wells x 30µl ea, 8-16 %, 10 gels £59.95<br />
Accessories<br />
BG-163 LongLife Tris-HEPES-SDS Running Buffer (1 x 5L) £34.00<br />
BG-145 Tris-Gly-SDS Running Buffer (1 x 5L) £34.00<br />
BG-165 Tris-HCl-SDS Sample Buffer (2x; 2 x 5 ml) £30.00<br />
Rapid Quick Coomassie Stain<br />
GEN-QC-Stain-1L Quick Coomassie Stain (1L) £88.00<br />
GEN-QC-Stain-3L Quick Coomassie Stain (3L) £225.00
Protein Ladders from Fermentas<br />
Order code Description Price<br />
GEN-SM1841 Spectra Broad Range Pre-stained Protein Ladder £98.50<br />
(10-260 kDa) 2 x 250 µl<br />
(Enough for 50 Mini gels (10 µl per well)<br />
GEN-SM1811 PageRuler Plus Pre-stained Protein Ladder £87.50<br />
(10-250 kDa) 2 x 250 µl<br />
(Enough for 100 Mini gels (5 µl per well)<br />
GEN-SM0671 PageRuler Pre-stained Protein Ladder £80.50<br />
(10-170 kDa) 2 x 250 µl<br />
(Enough for 100 Mini gels (5 µl per well)<br />
GEN-SM0661 PageRuler Unstained Protein Ladder £64.50<br />
(10-200 kDa) 2 x 250 µl<br />
(Enough for 100 Mini gels (5 µl per well)<br />
Micro-Dialysis Tubes from GeBA<br />
Order code Description Price<br />
Mini (10-250 µl)<br />
D070-6-10 GeBAflex-tube Mini (10-250 µl;10pc; 8kDa MWCO) £33.50<br />
D070-25-5 GeBAflex-tube Mini (10-250 µl;5pc; 25kDa MWCO) £33.00<br />
Midi (50-800 µl)<br />
D010 GeBAflex-tube Midi (50-800 µl;10pc; 3.5kDa MWCO) £52.00<br />
D020 GeBAflex-tube Midi (50-800 µl;10pc; 8kDa MWCO) £52.00<br />
Maxi (0.1-3 ml)<br />
D035 GeBAflex-tube Maxi (0.1-3 ml;15pc; 3.5kDa MWCO) £74.00<br />
D045 GeBAflex-tube Maxi (0.1-3 ml;15pc; 8kDa MWCO) £74.00<br />
D060-25-5 GeBAflex-tube Maxi (0.1-3 ml;5pc; 25kDa MWCO) £48.00<br />
D060-50-5 GeBAflex-tube Maxi (0.1-3 ml;5pc; 50kDa MWCO) £48.00<br />
Mega (3-20 ml)<br />
D080-1/10-5 GeBAflex-tube Mega (3-20 ml;5pc; 10 ml cap; 1kDa MWCO) £85.00<br />
D080-1/15-5 GeBAflex-tube Mega (3-20 ml;5pc; 15 ml cap; 1kDa MWCO) £85.00<br />
D080-1/20-5 GeBAflex-tube Mega (3-20 ml;5pc; 20 ml cap; 1kDa MWCO) £85.00<br />
D080-3.5/10-10 GeBAflex-tube Mega (3-20 ml;10pc; 10 ml cap; 3.5kDa MWCO) £85.00<br />
D080-3.5/15-10 GeBAflex-tube Mega (3-20 ml;10pc; 15 ml cap; 3.5kDa MWCO) £85.00<br />
D080-3.5/20-10 GeBAflex-tube Mega (3-20 ml;10pc; 20 ml cap; 3.5kDa MWCO) £85.00<br />
D080-6/10-10 GeBAflex-tube Mega (3-20 ml;10pc; 10 ml cap; 8kDa MWCO) £85.00<br />
D080-6/15-10 GeBAflex-tube Mega (3-20 ml;10pc; 15 ml cap; 8kDa MWCO) £85.00<br />
D080-6/20-10 GeBAflex-tube Mega (3-20 ml;10pc; 20 ml cap; 8kDa MWCO) £85.00
UltraPure Detergents and Reagents from Glycon<br />
(Bulk Available; No α-anomer)<br />
GEN-S-02122 Ultra pure IPTG > 99.0% (25 g) £82.50<br />
Non-Ionic Detergents<br />
Detergent Screening Kit (5 x 1 g of the following 5 ultrapure detergents)<br />
GEN-D2NM02 n-Decyl-β-maltoside (DM) > 99.5% (1 g) £200.00<br />
n-Dodecyl-β-maltoside (DDM) > 99.5% (1 g)<br />
n-Nonyl β-D-maltoside (NM) > 99.5% (1 g)<br />
n-Octyl β-D-glucopyranoside (OG) > 99.5% (1 g)<br />
n-Octyl 1-thio-β-D-glucopyranoside (OTG) > 99.5% (1 g)<br />
n-Octyl β-D-glucopyranoside (βOG)<br />
D97001-C-1 n-Octyl β-D-glucopyranoside (OG) > 99.5% (1 g) £35.00<br />
D97001-C-10 n-Octyl β-D-glucopyranoside (OG) > 99.5% (10 g) £225.00<br />
D97001-C-25 n-Octyl β-D-glucopyranoside (OG) > 99.5% (25 g) £425.00<br />
D97001-C-100 n-Octyl β-D-glucopyranoside (OG) > 99.5% (100 g) £1,400.00<br />
n-Decyl-β-maltoside (DM)<br />
D99003-C-1 n-Decyl-β-maltoside (DM) > 99.5% (1 g) £50.00<br />
D99003-C-10 n-Decyl-β-maltoside (DM) > 99.5% (10 g) £360.00<br />
D99003-C-25 n-Decyl-β-maltoside (DM) > 99.5% (25 g) £725.00<br />
D99003-C-100 n-Decyl-β-maltoside (DM) > 99.5% (100 g) £2,450.00<br />
n-Dodecyl-β-maltoside (DDM)<br />
D97002C-1 n-Dodecyl-β-maltoside (DDM) > 99.5% (1 g) £50.00<br />
D97002-C-10 n-Dodecyl-β-maltoside (DDM) > 99.5% (10 g) £360.00<br />
D97002-C-25 n-Dodecyl-β-maltoside (DDM) > 99.5% (25 g) £725.00<br />
D97002-C-100 n-Dodecyl-β-maltoside (DDM) > 99.5% (100 g) £2,450.00<br />
n-Octyl 1-thio-β-D-glucopyranoside<br />
D20014-1 n-Octyl 1-thio-β-D-glucopyranoside (OTG) > 99.5% (1 g) £35.00<br />
D20014-10 n-Octyl 1-thio-β-D-glucopyranoside (OTG) > 99.5% (10 g) £225.00<br />
D20014-25 n-Octyl 1-thio-β-D-glucopyranoside (OTG) > 99.5% (25 g) £425.00<br />
D20014-100 n-Octyl 1-thio-β-D-glucopyranoside (OTG) > 99.5% (100 g) £1,400.00<br />
n-Undecyl β-D-maltoside (UDM)<br />
D99012-C-25 n-Undecyl-β-maltoside (UDM) > 99.5% (25 g) £725.00<br />
D99012-C-100 n-Undecyl-β-maltoside (UDM) > 99.5% (100 g) £2,450.00<br />
n-Tridecyl β-D-maltoside (TDM)<br />
D20016-C-25 n-Tridecyl-β-maltoside (TDM) > 99.5% (25 g) £1,300.00<br />
D20016-C-100 n-Tridecyl-β-maltoside (TDM) > 99.5% (100 g) £4,200.00
n-Nonyl β-D-glucopyranoside (NG)<br />
D99011-C-25 n-Nonyl β-glucopyranoside (NG) > 99% (25 g) £425.00<br />
D99011-C-100 n-Nonyl β-glucopyranoside (NG) > 99% (100 g) £1,400.00<br />
n-Nonyl β-D-maltoside (NM)<br />
D20015-C-25 n-Nonyl β-D-maltoside (NM) > 99.5% (25 g) £725.00<br />
D20015-C-100 n-Nonyl β-D-maltoside (NM) > 99.5% (100 g) £2,450.00<br />
N-(n-Alkanoyl)-N-methylglucamines (MEGA)<br />
D98004-1 MEGA-8 >99.5%; N-Octanoyl (1 g) £30.00<br />
D98004-10 MEGA-8 >99.5%; N-Octanoyl (10 g) £155.00<br />
D98004-25 MEGA-8 >99.5%; N-Octanoyl (25 g) £308.00<br />
D98004-100 MEGA-8 >99.5%; N-Octanoyl (100 g) £980.00<br />
D99005-1 MEGA-9 >99.5%; N-Nonanoyl (1 g) £30.00<br />
D99005-10 MEGA-9 >99.5%; N-Nonanoyl (10 g) £155.00<br />
D99005-25 MEGA-9 >99.5%; N-Nonanoyl (25 g) £308.00<br />
D99005-100 MEGA-9 >99.5%; N-Nonanoyl (100 g) £980.00<br />
D99008-1 MEGA-10 >99.5%; N-Decanoyl (1 g) £30.00<br />
D99008-10 MEGA-10 >99.5%; N-Decanoyl (10 g) £155.00<br />
D99008-25 MEGA-10 >99.5%; N-Decanoyl (25 g) £308.00<br />
D99008-100 MEGA-10 >99.5%; N-Decanoyl (100 g) £980.00<br />
Ionic Detergent<br />
D99009-1 CHAPS >99 % (1 g) £42.00<br />
D99009-10 CHAPS >99 % (10 g) £78.00<br />
D99009-25 CHAPS >99 % (25 g) £170.00<br />
D99009-100 CHAPS >99 % (100 g) £582.00<br />
L-Selenomethionine, L-Selenocystine & 3-Iodo-L-Tyrosine<br />
3211-76-5 L-(+)-Selenomethionine > 98+% (2.5 g) £175.00<br />
50-50-250 L-Selenocystine > 98+% (250 mg) £38.00<br />
50-50-1000 L-Selenocystine > 98+% (1 g) £98.00<br />
70-78-0-1 3-Iodo-L-Tyrosine > 97+% (1 g) £40.00<br />
70-78-0-25 3-Iodo-L-Tyrosine > 97+% (25 g) £312.00<br />
VIP Purified Anti-Tag Antibodies<br />
Order code Description Price<br />
VIP-HIS-Mab His-Tag Ab (0.1 mg, mouse monoclonal IgG1) £119.00<br />
VIP-FLAG-Pab FLAG-Tag Ab (0.1 mg, polyclonal IgG) £119.00<br />
VIP-V5-Pab V5-Tag Ab (0.1 mg, polyclonal IgG) £119.00<br />
VIP-HA-Mab HA-Tag Ab (0.1 mg, mouse monoclonal IgG2b) £119.00<br />
VIP-GFP-Mab GFP-Tag Ab (0.1 mg, mouse monoclonal IgG1) £119.00<br />
VIP-MYC-Mab Myc-Tag Ab (0.1 mg, mouse monoclonal IgG1) £119.00
Elite Mouse and Rat Isotyping and Quantitation kit<br />
Order code Description Price<br />
GEN-20110 Rapid Type-M Mouse Isotyping Kit (10 tests) £169.00<br />
GEN-20210 Rapid Mouse IgG Quantitation Test kit (20 tests) £195.00<br />
GEN-20120 Rapid Type-R Rat Isotyping Kit (10 tests) £195.00<br />
Natural Lipids<br />
UltraPure Lipid Products<br />
Order code Description Price<br />
GEN-Cer-100 Cerebroside (100 mg) Grade 1 £59.40<br />
GEN-Eth-50 Ethanolamine phosphatides (50 mg) £59.40<br />
GEN-Gly-500 Glycerophosphorylcholine Free (500 mg) £158.40<br />
GEN-Gly-Cd-500 GPC Cadmium Chrloride comp. (500 mg) £44.10<br />
GEN-PC-BH-100 Phosphatidyl choline Bovine heart (100 mg) Grade 1 £76.50<br />
GEN-PC-BSC-100 Phosphatidyl choline Bovine spinal chord (100 mg) Grade 1<br />
£76.50<br />
GEN-PC-EY1-500 Phosphatidyl choline Egg Yolk (500 mg) Grade 1 £66.60<br />
GEN-PC-EY2a-500 Phosphatidyl choline Egg Yolk (500 mg) Grade 2a £52.20<br />
GEN-PC-EY2b-1000 Phosphatidyl choline Egg Yolk (1 g) Grade 2b £30.60<br />
GEN-PC-SB-250 Phosphatidyl choline Soya bean (250 mg) Grade 1 £52.20<br />
GEN-PC-EH-500 Phosphatidyl choline Egg, Hydrogenated (500 mg) Grade 1<br />
£65.70<br />
GEN-Lyso-100 Lysophophatidyl choline (100 mg) Grade 1 £56.70<br />
GEN-Lyso-PE-25 Lysophosphatidyl ethanolamine (25 mg) Grade 1 £54.90<br />
GEN-Lyso-PG-100 Lysophophatidyl glycerol (100 mg) Grade 1 £142.20<br />
GEN-PA-1-50 Phosphatidic acid (PA) (50 mg) Grade 1 £59.40<br />
GEN-PA-2-100 Phosphatidic acid (PA) (100 mg) Grade 2 £52.20<br />
GEN-PB-50 Phosphatidyl butanol (PB) (50 mg) £108.00<br />
GEN-PE-50 Phosphatidyl ethanol (50 mg) £108.00<br />
GEN-PEM-1-100 Phosphatidyl ethanolamine (PE) (100 mg) Grade 1 £93.60<br />
GEN-PEM-2-100 Phosphatidyl ethanolamine (PE) (100 mg) Grade 1 £61.20<br />
GEN-PG-1-50 Phosphatidyl glycerol (PG) (50 mg) Grade 1 £76.50<br />
GEN-PG-2-50 Phosphatidyl glycerol (PG) (50 mg) Grade 2 £61.20<br />
GEN-PI-50 Phosphatidyl inositol (PI) (50 mg) Grade 1 £85.50<br />
Order code Description Price<br />
GEN-PIBP-1 Phosphatidyl inositol 4,5-bisphosphate (1 mg) £277.20<br />
GEN-PIBP-5 Phosphatidyl inositol 4,5-bisphosphate (5 mg) £1,080.00<br />
GEN-PP-50 Phosphatidyl propanol (100 mg) £108.00<br />
GEN-PS-100 Phosphatidyl serine (100 mg) £93.60
Plant Leaf Lipids<br />
GEN-DGDG-50 Digalactosyl diglyceride (50 mg) £103.50<br />
GEN-MGDG-50 Monogalactosyl diglyceride (50 mg) £90.00<br />
GEN-PLL-PG Phosphatidyl glycerol (25 mg) £108.00<br />
GEN-SQDG Sulphoquinovosyl diglyceride (25 mg) £108.00<br />
GEN-SM Sphingomyelin (50 mg) Grade 1 £41.40<br />
GEN-S-25 Sulfatide (25 mg) Grade 1 £79.20<br />
GEN-S-100 Sulfatide (100 mg) Grade 1 £239.40<br />
GEN-TL-500 Total lipid extract, bovine spinal chord (500 mg) £54.90<br />
Our Grade 1 classification implies that we have seen no impurities when at least 100 µg<br />
lipid has been run on a thin layer plate and subsequently charred by a technique which<br />
reveals the presence of 1 µg lipid. Grade 2 products are made either by simpler methods<br />
or batches which just fail to pass our tests as Grade 1.<br />
Semi-Synthetic Lipids<br />
Order code Description C:∆ Price<br />
GEN-DAG-100 Diacyl glycerol (100 mg) Grade 1 Mixed £131.40<br />
GEN-DLPC-100 Dilauroyl PC (100 mg) Grade 1 12:0 £54.90<br />
GEN-DLL-100 Dilinoleoyl PC (100 mg) Grade 1 18:2 £54.90<br />
GEN-DMPC-100 Dimyristoyl PC (100 mg) Grade 1 14:0 £54.90<br />
GEN-DOPC-100 Dioctanoyl PC (100 mg) Grade 1 8.0 £54.90<br />
GEN-DOG-100 Dioleoyl glycerol (100 mg) Grade 1 18:1 £131.40<br />
GEN-DOLPC-100 Dioleoyl PC (100 mg) Grade 1 18:1 £54.90<br />
GEN-DOPE-50 Dioleoyl PE (50 mg) Grade 1 18:1 £131.40<br />
GEN-DPPC-100 Dipalmitoyl PC (100 mg) Grade 1 16:0 £54.90<br />
GEN-DPPE-50 Dipamitoyl PE (50 mg) Grade 1 16:0 £131.40<br />
GEN-DSPC-100 Distearoyl PC (100 mg) Grade 1 18:0 £54.90<br />
GEN-LLPC-50 1-Lauroyl-2-lysoPC (50 mg) Grade 1 12:0 £81.00<br />
<strong>Generon</strong> Ltd.,12 Rawcliffe House, Howarth Rd, Maidenhead, Berkshire SL6 1AP.<br />
Order and Technical Support Tel: (01753) 866-511 Order Fax: (01753) 20-88-99<br />
Order E-mail: info@generon.co.uk WWW: www.generon.co.uk<br />
*All prices exclude VAT and are FOB <strong>Generon</strong> UK
Protein�Consumable�Offers:�<br />
����������������������������������Much�more�at�the�<strong>Generon</strong>�website!� Vivaspins,�Vivacells�and�Vivaflows��<br />
� Price�Checker<br />
Guaranteed�best�pricing�in�UK!��<br />
�<br />
Order�one�of�our�special�priced��<br />
Vivascience�products�eg�VS2002��<br />
(see�website)�and�enjoy�a�further��<br />
<strong>Generon</strong>�prices�compared�to�other�<br />
suppliers�for�a�Vivaspin�20�pack,�<br />
(48�pc,�10�kDa�MWCO;�VS2002)�<br />
�<br />
10%�discount�off�our�� �������������<strong>Generon</strong>�price������£178.88�<br />
catalogue�prices!� Fisher�list�price������£354.63�<br />
� VWR�list�price������£297.90�<br />
�<br />
�<br />
�<br />
Nusep�Protein�Gels:�The�most�affordable�pre�cast�protein�gels�on�the�market�<br />
£59.95�for�a�pack�of�10�gels.�Unparalleled�performance�and�up�to�half�price�<br />
compared�to�those�from�Bio�Rad�and�Invitrogen.�<br />
Available�as�8%,�10%,�12%,�4�12%�and�8�16%�Tris�Glycine��or�Tris�HEPES�gels�<br />
Innovative�3�min.�UV�visualization�comes�as�standard�with�all�Nusep�gels.�<br />
Compatible�with�standard�(10�cm)�mini�gel�tanks�including�Invitrogen,�Bio�<br />
Rad�and�Hoeffer.�Min.�1�year�shelf�life!�<br />
Recombinant�Protein�A�and�Protein�G�Sepharose�FF�resin�for�Immuno�precipitations<br />
Sartorius�list�price������£306.90�<br />
�<br />
5�ml�recombinant�Protein�A�or�Protein�G�Sepharose�for�only�£210.��<br />
Compare�these�prices�with�those�from�GE.�<br />
High�Quality�immobilized�recombinant�Protein�A�and�G�Sepharose�FF�resin.�<br />
Selectively�optimized�for�your�IP�work.�The�resins�are�screened�through�a�300�µm�filter�to�remove�clumps.�<br />
�<br />
�<br />
�<br />
<strong>Generon</strong> Ltd,<br />
� 12 Rawcliffe House, Howarth Road,<br />
Tel:�� (01753)�866�511� Maidenhead,<br />
Fax:� (01753)�20�88�99�<br />
E�mail:�� info@generon.co.uk�<br />
Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk<br />
�<br />
�
Detergent�and�Lipid�Product�Offers:�<br />
��������������������������������Much�more�at�the�<strong>Generon</strong>�website!� Ultra�Pure�alkyl���glycoside�detergents:�<br />
�<br />
��DDM,���DM,�OG,�OTG,�MEGA�etc�for...�<br />
�<br />
� Protein�Purifications�<br />
� Reconstitutions�<br />
� Crystallizations�<br />
�<br />
�<br />
�<br />
No���anomer�in�our�Glycon�detergents.��<br />
All�our�detergents�are�crystallized�to��<br />
greater�than�99.5%�purity.�<br />
�<br />
Guaranteed�to�be�better�quality�and�more�<br />
affordable�than�Anatrace!�Used�by�many��<br />
top�membrane�protein�laboratories�in�UK.�<br />
�<br />
�<br />
Ultra�Pure�Natural�and�Semi�Synthetic�Lipids�from�<strong>Generon</strong>:�<br />
�PC,�PS,�PA,�PG,�PI,�PIP ,�DOPC,�DOPE,�DMPC�etc�for:�<br />
2<br />
�<br />
�<br />
�<br />
�<br />
� Micelles�<br />
� Liposome�Research�<br />
� Signal�Transduction�Studies�<br />
The�purest�and�most�affordable�Grade�1�lipids�on�the�market.�<br />
Tel:�� (01753)�866�511�<br />
Fax:� (01753)�20�88�99�<br />
E�mail:�� info@generon.co.uk�<br />
�<br />
� Chromatogram<br />
showing high<br />
purity of �-<br />
DDM from<br />
Glycon<br />
� �<br />
Superior�quality,�fast�delivery�times�and�no�freight�charges�with�all�our�lipid�purchases!�<br />
<strong>Generon</strong> Ltd,<br />
12 Rawcliffe House, Howarth Road,<br />
Maidenhead,<br />
Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk<br />
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�
Cell Culture + Filtration<br />
Cell Culture & UF Concentration<br />
-Vivascience/Sartorius Products eg Minisart syringe filters, Vivaspins, Vivacells, Vivaflows,<br />
Vivapures<br />
-BJP Static Urine Concentrators<br />
-UF Membrane Discs for Amicon Stirred Cells<br />
-Ultra Yield Flasks from Thompson (125 ml-2.5 L)<br />
-AlamarBlue Stain from Trek Diagnostic Systems (25 ml, 100 ml)<br />
-Avetex 3-D Cell Culture from Reinnervate (12 well plates, 6 well & 12 well inserts)<br />
-CELLine 350 and CELLine 1000 Two-compartment Bioreactors from Integra<br />
Cell Culture Reagents<br />
-Serum e.g. FCS<br />
-Synthetic Media, powdered and liquid e.g. DMEM, RPMI<br />
-Salt solutions and Buffers e.g. PBS<br />
-Amino Acids e.g. L-glutamine<br />
-Antibiotics e.g. Penicillin-Streptomycin<br />
-Vitamins e.g. L-ascorbic acid<br />
-Other Supplements e.g. Trypsin, Trypsin-EDTA<br />
Filterware<br />
-Nalgene Mach V Supor Membrane Vacuum Filtration Units<br />
-Proteus Mini Clarification Spin Columns from <strong>Generon</strong><br />
-Filter Units and Cell Culture Plates from Thomson
Vivaspin Ultrafiltration Family –<br />
sample concentration up to 20 ml<br />
Vivaspin centrifugal concentrators offer<br />
the optimal solution to any concentration<br />
or buffer exchange application with<br />
their broad range of volume capacities<br />
and MWCOs.<br />
Highest flow rates and recoveries are<br />
achieved due to their particular vertical<br />
membrane design.<br />
Incomparably safe sample handling and<br />
convenience is assured by the patented<br />
dead-stop technology allowing direct<br />
recovery of the concentrate without<br />
further respin.<br />
Feature Benefit<br />
Vivaspin applications<br />
– Concentration of biological samples<br />
containing antigens, antibodies,<br />
enzymes or nucleic acids<br />
– Concentration of diluted protein<br />
samples from chromatography eluates<br />
– Desalting or Diafiltration |<br />
Buffer exchange<br />
– Sample concentration for crystallization<br />
and NMR spectroscopy<br />
– Removal of very small or large<br />
contaminants<br />
Vertical, large membrane design Fast, minimal membrane blocking<br />
Integrated Dead-Stop-Volume No risk (to concentrate to dryness)<br />
Low-binding materials High recovery<br />
No Re-spin necessary Easy handling, fast<br />
Membrane selection Highest recovery with extremely<br />
low-binding Hydrosart ® membrane in<br />
Vivaspin 2 and Vivaspin 15R<br />
Broad volume range from 0.6 – 100 ml High volume flexibility<br />
Wide MWCO range from 2 kDa – 0.2 µm High MWCO flexibility<br />
Special Devices<br />
Vivascience offers an extended range of<br />
membranes to cover the different ultrafiltration<br />
requirements which will typically<br />
yield recoveries of the concentrated<br />
sample in excess of 90% when the starting<br />
sample contains over 0.1 mg/ml<br />
of the solute of interest.<br />
For highest recovery, select a membrane<br />
MWCO which is at least half to one third<br />
of the molecular weight of the solute to<br />
be retained.<br />
Highest recovery with extremely<br />
low binding Hydrosart ® membrane<br />
– The membrane of choice for antibody<br />
concentration<br />
– MWCO range from 2 kDa – 30 kDa<br />
– Available in Vivaspin 2 and<br />
Vivaspin 15R<br />
– Vivaspin 2 offers choice of membranes:<br />
PES, Hydrosart ® and CTA<br />
Vivaspin Ultrafiltration Family<br />
Vivaspin 500<br />
600 µl* – 5 µl*<br />
Vivaspin 4<br />
4 ml – 20 µl<br />
Vivaspin 15<br />
15 ml – 50 µl<br />
Vivaspin 20<br />
20 ml – 50 µl<br />
*Volume **Dead stop vol.<br />
Vivaspin 2<br />
2 ml – 8 µl<br />
Vivaspin 6<br />
6 ml – 30 µl<br />
Vivaspin 15R<br />
15 ml – 50 µl
Ordering information –<br />
Vivaspin<br />
Ordering information<br />
Vivaspin – PES Membrane<br />
MWCO 3kDa 5kDa 10kDa 30kDa 50kDa<br />
Product Volume Dead stop vol.<br />
Vivaspin 500 0.6 ml 5 µl VS0191 (25)* VS0111 (25) VS0101 (25) VS0121 (25) VS0131 (25)<br />
VS0192 (100) VS0112 (100) VS0102 (100) VS0122 (100) VS0132 (100)<br />
Vivaspin 2 2 ml 8 µl VS0291 (25) VS0211 (25) VS0201 (25) VS0221 (25) VS0231 (25)<br />
VS0292 (100) VS0212 (100) VS0202 (100) VS0222 (100) VS0232 (100)<br />
Vivaspin 4 4 ml 20 µl VS0413 (25) VS0403 (25) VS0423 (25) VS0433 (25)<br />
VS0414 (100) VS0404 (100) VS0424 (100) VS0434 (100)<br />
Vivaspin 6 6 ml 20 µl VS0691 (25) VS0611 (25) VS0601 (25) VS0621 (25) VS0631 (25)<br />
VS0692 (100) VS0612 (100) VS0602 (100) VS0622 (100) VS0632 (100)<br />
Vivaspin 15 15 ml 50 µl VS1511 (10) VS1501 (10) VS1521 (10) VS1531 (10)<br />
VS1512 (40) VS1502 (40) VS1522 (40) VS1532 (40)<br />
Vivaspin 20 20 ml 50 µl VS2091 (12) VS2011 (12) VS2001 (12) VS2021 (12) VS2031 (12)<br />
VS2092 (48) VS2012 (48) VS2002 (48) VS2022 (48) VS2032 (48)<br />
MWCO 100kDa 300kDa 1,000kDa 0.2 µm 5 of each<br />
Product Volume Dead stop vol.<br />
Vivaspin 500 0.6 ml 5 µl VS0141 (25) VS0151 (25) VS0161 (25) VS0171 (25) VS01S1 (25)<br />
VS0142 (100) VS0152 (100) VS0162 (100) VS0172 (100)<br />
Vivaspin 2 2 ml 8 µl VS0241 (25) VS0251 (25) VS0261 (25) VS0271 (25) VS02S1 (25)<br />
VS0242 (100) VS0252 (100) VS0262 (100) VS0272 (100)<br />
Vivaspin 4 4 ml 20 µl VS0443 (25) VS0473 (25) VS04S3 (25)<br />
VS0444 (100) VS0474 (100)<br />
Vivaspin 6 6 ml 20 µl VS0641 (25) VS0651 (25) VS0661 (25) VS0671 (25) VS06S1 (25)<br />
VS0642 (100) VS0652 (100) VS0662 (100) VS0672 (100)<br />
Vivaspin 15 15 ml 50 µl VS1541 (10) VS1571 (10) VS15S1 (10)<br />
VS1542 (40) VS1572 (40)<br />
Vivaspin 20 20 ml 50 µl VS2041 (12) VS2051 (12) VS2061 (12) VS2071 (12) VS20S1 (12)<br />
VS2042 (48) VS2052 (48) VS2062 (48) VS2072 (40)<br />
Vivaspin – Hydrosart ® Membrane<br />
MWCO 2kDa 5kDa 10kDa 30kDa<br />
Product Volume Dead stop vol.<br />
Vivaspin 2 2 ml 5 µl VS02H91 (25)* VS02H11 (25) VS02H01 (25) VS02H21 (25)<br />
VS02H92 (100) VS02H12 (100) VS02H02 (100) VS02H22 (100)<br />
Vivaspin 15R 15 ml 30 µl VS15RH91 (12) VS15RH11 (12) VS15RH01 (12) VS15RH21 (12)<br />
VS15RH92 (48) VS15RH12 (48) VS15RH02 (48) VS15RH22 (48)<br />
Vivaspin – CTA Membrane<br />
MWCO 5kDa 10kDa 20kDa<br />
Product Volume Dead stop vol.<br />
Vivaspin 2 2 ml 5 µl VS02U1 (25) VS02V1 (25) VS02X1 (25)<br />
VS02U2 (100) VS02V2 (100) VS02X2 (100)<br />
Vivaspin Additional Devices<br />
Product Description Pack size Order No.<br />
Vivaspin 20 – Diafiltration cups 12 VSA005<br />
*(pack size in parenthesise)
Vivacell Pressure Units<br />
Sample concentration up to 250 ml l<br />
Vivacell 70 – Centrifugation Pressure<br />
70 ml* – (150 µl)**<br />
Vivacell 100 – Centrifugation Pressure<br />
100 ml* – (350 µl)**<br />
Vivacell 250 – Pressure<br />
250 ml* – (600 µl)**<br />
*Volume **Dead stop vol.<br />
Vivacell – Put your proteins under<br />
pressure! Pressure, Centrifuge,<br />
Shaking – the choice is yours<br />
Vivascience offers the Vivacell product<br />
range of gas pressure and centrifugal<br />
concentrators for sample volumes from<br />
20 ml to 250 ml.<br />
The patented vertical membrane design<br />
guarantees highest performance and<br />
unmatched flexibility.<br />
Vivacell is a unique and innovative<br />
concentrator, that utilizes pressure,<br />
centrifuge, shaking or pressure-shake<br />
to rapidly concentrate even samples<br />
with high particle loading.<br />
Benefit from patented dead-stop<br />
technology, high process speed and<br />
recovery, direct recovery without<br />
reverse spin!
Ordering information –<br />
Vivacell<br />
Ordering information<br />
Vivacell – PES Membrane<br />
MWCO 5kDa 10kDa 30kDa 50kDa<br />
Product Volume Dead stop vol.<br />
Vivacell 70 70 ml 150 µl VS6011 (2)* VS6001 (2) VS6021 (2) VS6031 (2)<br />
VS6012 (10) VS6002 (10) VS6022 (10) VS6032 (10)<br />
Vivacell 100 100 ml 350 µl VC1011 (2) VC1001 (2) VC1021 (2) VC1031 (2)<br />
VC1012 (10) VC1002 (10) VC1022 (10) VC1032 (10)<br />
Vivacell 250 250 ml 600 µl VC2511 (5) VC2501 (5) VC2511 (5) VC2531 (5)<br />
MWCO 100kDa 300kDa 1,000kDa 0.2 µm<br />
Product Volume Dead stop vol.<br />
Vivacell 70 70 ml 150 µl VS6041 (2) VS6071 (2)<br />
VS6042 (10) VS6072 (10)<br />
Vivacell 100 100 ml 350 µl VC1041 (2) VC1051 (2) VC1061 (2) VC1071 (2)<br />
VC1042 (10) VC1052 (10) VC1062 (10) VC1072 (10)<br />
Vivacell 250 250 ml 600 µl VC2541 (5) VC2571 (5)<br />
Vivacell additional devices<br />
Product description Pack size Order no.<br />
Vivacell 70<br />
Vivacell 70 centrifuge bottle 4 VCA003<br />
Vivacell 70 bottles with caps 1 VCA004<br />
Vivacell 70 replacement seals 1 VCA007<br />
Vivacell 70 pressure head with bottle 1 VCA700<br />
Vivacell 70 pressure-fuge head 2 VCA701<br />
Vivacell 100<br />
Vivacell 100 concentrator cap 2 VCA013<br />
Vivacell 100 santoprene replacement seals 10 VCA014<br />
Vivacell 100 pressure head 1 VCA800<br />
Vivacell 250<br />
Vivacell 250 complete set 1 VCA250<br />
Vivacell 250 PES 5 EA MWCO 5 VC25S1<br />
Vivacell 250 maintenance kit 1 VCA009<br />
Common pressure accessories<br />
Air Pressure Controller (APC) 1 VCA002<br />
Charge valve for pressure-fuge head 1 VCA005<br />
Female coupling quick disconnect 1 VCA010<br />
Male tube coupling 1 VCA011<br />
4mm OD pressure tube 3m 1 VCA012<br />
Pressure inductor pressure valve 1 VCA008<br />
Replacement pressure head and screw closure 1 VCA015<br />
*(pack size in parenthesise)
Vivaflow Crossflow Cassettes<br />
Sample concentration up to 5 l<br />
Vivaflow 50<br />
1 l*– 10 ml**<br />
Vivaflow 200<br />
5 l – 20 ml<br />
*Volume **Dead stop vol.<br />
Vivaflow setup<br />
Vivaflow 50 – Multiple modules<br />
Vivaflow 50 – Single module<br />
Filtrate vessel<br />
Sample | diafiltration<br />
reservoir (VFA006)<br />
Applications<br />
– Concentration of cell culture<br />
supernatants<br />
– Concentration of viruses, bacteria, etc<br />
– Concentration of trace metals<br />
from seawater
Ordering information<br />
Vivaflow – PES Membrane<br />
MWCO 5kDa 10kDa 30kDa 50kDa<br />
Product Volume Min vol.<br />
Vivaflow 50 1 l 10 ml VF05P1 (2)* VF05P0 (2) VF05P2 (2) VF05P3 (2)<br />
Vivaflow 200 5 l 20 ml VF20P1 (1) VF20P0 (1) VF20P2 (1) VF20P3 (1)<br />
MWCO 100kDa 0.2 µm<br />
Product Volume Min vol.<br />
Vivaflow 50 1 l 10 ml VF05P4 (2) VF05P7 (2)<br />
Vivaflow 200 5 l 20 ml VF20P4 (1) VF20P7 (1)<br />
Vivaflow – RC Membrane<br />
MWCO 10kDa 100kDa<br />
Product Volume Min vol.<br />
Vivaflow 50 1 l 10 ml VF05C0 (2)* VF05C4 (2)<br />
Vivaflow 200 5 l 20 ml VF20C0 (1) VF20C4 (1)<br />
Vivaflow – Hydrosart ® Membrane<br />
MWCO 5kDa 10kDa 30kDa<br />
Product Volume Min vol.<br />
Vivaflow 200 5 l 20 ml VF20H1 VF20H0 VF20H2<br />
Vivaflow – Complete system (pump, easy load pump head & tubing)<br />
Product Description Order No.<br />
Vivaflow 50 VFS504<br />
Vivaflow 200 VFS204<br />
*(pack size in parenthesise)
FACT: The most<br />
competitivelypriced<br />
ultrafiltration<br />
membrane<br />
discs in the<br />
market<br />
Ultrafiltration Membrane Discs<br />
For all standard stirred cells<br />
<strong>Generon</strong>’s ultrafiltration membranes are a series of anisotropic<br />
membranes specially selected following research into the<br />
performances of existing membrane discs in the market.<br />
The membrane has been proprietary-treated to ensure low non-specific<br />
protein binding at higher speed.<br />
The membrane discs are available in a complete range of diameters for<br />
stirred cells and for other filtration products which require ultrafiltration membranes.<br />
The hydrophilic regenerated cellulose membrane discs offer superior<br />
chemical resistance and are preferred for highest protein<br />
recovery when handling dilute protein solutions.<br />
The polyethersulphone membrane discs have a more open structure which provides fast separation<br />
flux. Use the polyethersulphone membranes for fast protein separation over a broad pH range.<br />
Specifications<br />
Membrane material: Proprietary-treated polyethersulphone (PES) and<br />
Regenerated cellulose (RC)<br />
MWCOs (molecular weight cut-offs): 5 kDa, 10 kDa, 30 kDa, 50 kDa, 100 kDa (PES)<br />
10 kDa, 30 kDa (RC)<br />
Filter disc diameter (mm): 25 mm, 44.5 mm, 62 mm, 76 mm, 90 mm<br />
Maximum operating pressure: 75 psi (5 bar)<br />
Maximum operating temperature: 60 ˚C<br />
Working pH range: pH 1-14 (PES)<br />
pH 3-12 (RC)<br />
Storage: Store at room temperature<br />
Membrane wetting agent: Shipped dry in glycerine and sodium azide<br />
Storage after use: Store in 10 % ethanol made up in distilled water or<br />
0.1 % sodium azide at +4 ˚C<br />
Sterilization: Sterilize using 70 % ethanol or a mixture of 0.5 %<br />
formalin and 25 % ethanol or 5 % formalin.<br />
Cleaning membrane discs: Rinse the PES or RC membrane in 0.1 M NaOH,<br />
Quantity of discs per pack: 10<br />
100 ppm NaOCl followed by washing with copious<br />
amounts of distilled water. For strongly bound<br />
proteins, soak the membrane in 0.1 % protease<br />
solution and then wash the membrane thoroughly<br />
with distilled water.
Part Numbers<br />
Filter diameter<br />
(mm)<br />
LIST PRICES FOR STIRRED CELLS<br />
RC* PRICE (excl. VAT) PES** PRICE (excl. VAT)<br />
5,000 MWCO<br />
25 mm (10 discs) PES-25-05 £55.00<br />
44.5 mm (10 discs) PES-44-05 £70.00<br />
62 mm (10 discs) PES-62-05 £80.00<br />
76 mm (10 discs) PES-76-05 £94.00<br />
90 mm (10 discs) PES-90-05 £99.00<br />
10,000 MWCO<br />
25 mm (10 discs) RC-25-10 £60.00 PES-25-10 £55.00<br />
44.5 mm (10 discs) RC-44-10 £75.00 PES-44-10 £70.00<br />
62 mm (10 discs) RC-62-10 £90.00 PES-62-10 £80.00<br />
76 mm (10 discs) RC-76-10 £102.00 PES-76-10 £94.00<br />
90 mm (10 discs) RC-90-10 £110.00 PES-90-10 £99.00<br />
30,000 MWCO<br />
25 mm (10 discs) RC-25-30 £60.00 PES-25-30 £55.00<br />
44.5 mm (10 discs) RC-44-30 £75.00 PES-44-30 £70.00<br />
62 mm (10 discs) RC-62-30 £90.00 PES-62-30 £80.00<br />
76 mm (10 discs) RC-76-30 £102.00 PES-76-30 £94.00<br />
90 mm (10 discs) RC-90-30 £110.00 PES-90-30 £99.00<br />
50,000 MWCO<br />
25 mm (10 discs) PES-25-50 £55.00<br />
44.5 mm (10 discs) PES-44-50 £70.00<br />
62 mm (10 discs) PES-62-50 £80.00<br />
76 mm (10 discs) PES-76-50 £94.00<br />
90 mm (10 discs) PES-90-50 £99.00<br />
100,000 MWCO<br />
25 mm (10 discs) PES-25-100 £55.00<br />
44.5 mm (10 discs) PES-44-100 £70.00<br />
62 mm (10 discs) PES-62-100 £80.00<br />
76 mm (10 discs) PES-76-100 £94.00<br />
90 mm (10 discs) PES-90-100 £99.00<br />
* RC: Regenerated Cellulose ** PES: Polyethersulphone<br />
Other products sold by <strong>Generon</strong>:<br />
<strong>Generon</strong> is an authorized UK distributor for Pro-Chem, Sartorius, Sterogene, Glycon, Tubewriter,<br />
Biosera. Please visit the <strong>Generon</strong> website (www.generon.co.uk) for information about ultra pure<br />
carbohydrate detergents, lipid products and other protein consumables.<br />
Tel: (01753) 866-511<br />
Fax: (01753) 20-88-99<br />
E-mail: info@generon.co.uk<br />
For more information or to order, contact <strong>Generon</strong>:<br />
<strong>Generon</strong> Ltd,<br />
12 Rawcliffe House, Howarth Road,<br />
Maidenhead,<br />
Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk
Alvetex-3D Advantage<br />
Alvetex 3-D Cell Culture<br />
alvetex ® is a highly porous<br />
polystyrene scaffold engineered<br />
into a 200μm thick membrane<br />
which provides a 3D space into<br />
which cells can invade, proliferate<br />
and grow. The vertical axis of the<br />
third dimension essentially enables<br />
additional layers of cells to form<br />
complex 3D interactions with<br />
adjacent cells simulating the<br />
structure of a tissue.<br />
alvetex ® can be used for routine 3D cell culture, as an inert plastic.<br />
alvetex ® is a 3D culture product which can be treated in the same manner<br />
as traditional 2D cell culture plastic. The scaffold can be plasma-treated<br />
without any detrimental effect to its structure.<br />
alvetex ® can be sterilised using gamma radiation. The use of polystyrene<br />
as a cell growth substrate is well accepted and recognised.<br />
Order code Description Price<br />
AVP002 12 well Alvetex ® plate (1 pc) £78.00<br />
AVP015 Alvetex ® well insert holder and petri-dish (1 pc) £26.00<br />
AVP-004-3 Alvetex ® 6 well insert (3 pc) £39.00<br />
AVP-005-3 Alvetex ® 12 well insert (3 pc) £33.00
Alvetex ®<br />
Scaffold<br />
ADD A NEW<br />
DIMENSION<br />
TO YOUR CELL<br />
CULTURE<br />
RESEARCH<br />
Takeyourcellculturebeyondthe<br />
limitationsofthemono-layer!<br />
Culturing cells in 3-dimensions using<br />
Alvetex ® Scaffold technology delivers<br />
powerful improvements over traditional<br />
2-dimensional mono-layer cultures.<br />
Cells biologists can maintain the integrity<br />
of cell structure and organisation found<br />
within the native tissue environments to<br />
accelerate understanding of real cell<br />
function and behaviour.<br />
Alvetex ® voted<br />
TOP 100 LIFE SCIENCE<br />
INNOVATIONS OF 2010<br />
by the Scientist Magazine<br />
Alvetex ® voted<br />
TOP 100 INNOVITIVE<br />
PRODUCTS 2011<br />
by R&D 100 Awards<br />
Alvetex ® Scaffold viewed by scanning<br />
electron microscope; a 200um thick<br />
polystyrene membrane (>90% porosity)<br />
polystyrene membrane<br />
REAL 3D cell culture delivers<br />
real benefits to your research:<br />
• Enrich the biological relevance of your cell culture<br />
based research<br />
• Deepen your understanding of in-vivo cell function<br />
and behaviour<br />
• Understand how cells interact within complex<br />
systems<br />
• Enhance primary cell viability and responsiveness to<br />
growth factors<br />
• Build in-vitro assays which much more accurately<br />
represent the natural cell (in-vivo) environment<br />
REAL 3D cell culture, simply and routinely<br />
Tissue processing and staining<br />
highlighting the manner in which cells<br />
freely penetrate the scaffold and form<br />
complex organizations throughout the<br />
Alvetex ® Scaffold matrix.
Accelerate your research and discovery.<br />
Alvetex ® Scaffold delivers<br />
real benefits to your research.<br />
In 2D culture, cells are forced to adapt; structural changes to the<br />
cytoskeleton result in a flattened morphology. These changes<br />
impair cellular functions and drastically reduce the opportunity<br />
for cell - cell interactions.<br />
A Hassle Free introduction<br />
to 3D Cell Culture<br />
Available in a range of Plate and Well Insert formats<br />
Alvetex ® Scaffold provides the ideal environment for cells to<br />
penetrate, grow and proliferate in 3 dimensions (3D). All<br />
products are made from the same inert polystyrene material<br />
as standard 2D cell culture plasticware. Cell Biologists can<br />
quickly experience the many benefits of 3-dimensional cell<br />
culture and at the same time easily switch between 2D and<br />
3D experiments.<br />
Cells maintain their natural 3D shape and structure within<br />
Alvetex ® Scaffold, freely interacting with adjacent cells and accurately<br />
mimicking the natural tissue organisation and environment.<br />
Additional Features and Benefits of<br />
Alvetex ® Scaffold include:<br />
• Compatible with the majority of coating reagents<br />
• Inert & Stable – no new experimental variables, ideal for<br />
long term cell culture<br />
• 90% Scaffold Porosity – cells can easily penetrate scaffold<br />
and cell retrieval possible<br />
• No changes required to already established sera, media<br />
and growth reagents<br />
• No additional equipment required for routine use<br />
Alvetex ®<br />
Scaffold - available in a range of formats to meet the needs of your assay<br />
Alvetex ® Scaffold<br />
12 well plate format<br />
Code Name<br />
AVP002 Alvetex®Scaffold 12-well plate, 1 plate<br />
AVP004-3 Alvetex®Scaffold 6-well inserts, 3 inserts<br />
AVP005-3 Alvetex®Scaffold 12-well inserts, 3 inserts<br />
AVP015 Alvetex®Scaffold well insert holder with deep Petri dish<br />
BN0224123<br />
Alvetex ® Scaffold 6 and<br />
12 Well Insert format<br />
Deep Petri dish and<br />
Well Insert Holder<br />
Free evaluation<br />
packs now<br />
available!<br />
To arrange for a FREE EVALUATION<br />
of Alvetex ® Scaffold simply contact:<br />
info@generon.co.uk<br />
www.reinnervate.com
Cell Culture<br />
CELLine<br />
Two-Compartment Bioreactor for Efficient Cell Cultivation<br />
Description<br />
The CELLine bioreactor is a disposable,<br />
two-compartment cultivation device suitable<br />
for many cell culture applications, e. g. the<br />
production of monoclonal antibodies on a<br />
laboratory scale. Two different sizes of the<br />
CELLine are available: CELLine CL350 and<br />
CL 1000. Details are listed below.<br />
Introduction<br />
Efficient cell cultivation is dependent on<br />
an optimal supply of oxygen and nutrients,<br />
as well as an efficient removal of inhibiting<br />
metabolic waste products. In static cell<br />
culture the optimal balance of these factors<br />
is not given. These limitations lead to a<br />
reduced cell growth and are unfavorable for<br />
achieving high levels of protein expression.<br />
Features & Benefits<br />
The two-compartment bioreactor CELLine<br />
is designed to overcome these limitations<br />
by dividing the bioreactor into a medium<br />
compartment and a cell compartment. A<br />
semi-permeable membrane (10 kDa MWCO)<br />
between the compartments allows small<br />
molecules to diffuse from one compartment<br />
to the other. Higher molecular weight molecules<br />
secreted by the proliferating cells are<br />
retained within the cell compartment. This<br />
results in a continuous flow of nutrients<br />
into the cell compartment and a concurrent<br />
removal of any inhibitory waste products.<br />
The cell compartment and the medium compartment<br />
are individually accessible which<br />
enables the cells to be supplied with fresh<br />
medium according to their individual needs.<br />
In addition, the bottom of the cell compartment<br />
is made of a gas permeable membrane<br />
which ensures an optimal oxygen supply<br />
to and carbon dioxide exchange from the<br />
cell culture. All together, this bioreactor<br />
construction allows optimal maintenance<br />
of the cells at quasi in vivo cultivation<br />
conditions. These conditions enable cells<br />
to proliferate to very high densities within<br />
the cell compartment.<br />
Application<br />
The CELLine is perfectly suited for a wide<br />
range of applications involving suspension<br />
cell culture, like monoclonal antibody<br />
production or long-term continuous culture<br />
maintenance. The unit is optimised for<br />
cultivation of hybridoma, CHO (Chinese<br />
hamster ovary) cells, NSO (mouse myeloma)<br />
cells, SF (Spodoptera frugiperda, insect cells)<br />
cells and many more.<br />
High Cell Density Cultivation<br />
With the two-compartment technology of<br />
CELLine, deficiencies in nutrition or oxygenation<br />
are problems of the past. Therefore,<br />
cell densities inside the cell compartment are<br />
usually between 10 7 to 10 8 cells per ml which<br />
is about two magnitudes higher than with<br />
conventional static cell culture techniques.<br />
High product concentrations<br />
Benefiting from the high cell densities,<br />
product concentrations achieved in CELLine<br />
are in the range of 1 to 5 mg per ml, making<br />
the bioreactor most suitable for antibody<br />
expression in hybridomas, protein expression<br />
in transfected cell lines, or virus production.<br />
Product concentrations in the cell compartment<br />
of CELLine are in most cases 50–100<br />
times above those found in static cell culture<br />
disposables, such as flasks and roller bottles.<br />
Long-term, continuous culture<br />
maintentance<br />
Separate access to the medium compartment<br />
of CELLine allows for continuous delivery<br />
and efficient oxygenation of the cells, thus<br />
enabling the user to perform continuous<br />
studies on a specific cell culture, or to benefit<br />
from a consistent protein production over<br />
several weeks.
NALGENE ®<br />
TM<br />
MF75 Filters with<br />
Pall Supor ®<br />
machV PES Membranes<br />
Provide You with Faster Filtration<br />
PES<br />
• Low protein binding and low extractables make<br />
PES the ideal membrane for your critical media<br />
sterilization<br />
• NALGENE MF75 filters with fast-flowing Supor<br />
machV PES membrane can make your lab more<br />
productive<br />
• Plus, you get all the other proven features of<br />
NALGENE MF75 filters<br />
– Easy-to-use ergonomic shape<br />
– Lot identification right on the product<br />
– Widest choice of filter units and bottle-top<br />
sizes<br />
– Guaranteed leakproof closure system<br />
– NALGENE-certified performance<br />
NALGENE, the leader in laboratory products, is leading the development of faster, more productive<br />
filtration. Only NALGENE MF75 filter units and bottle-top filters are available with the exclusive 0.2µm<br />
Supor machV membrane technology from Pall. Supor machV is an extremely clean, fast-flowing,<br />
asymmetric PES membrane that provides outstanding throughput and low protein binding of media<br />
and other solutions. Combining the manufacturing and laboratory expertise of NALGENE with the<br />
membrane technology from Pall gives you fast, reliable, and easy-to-use vacuum filtration systems.<br />
www.NALGENElabware.com
NALGENE MF75 with Supor machV PES Filters Faster<br />
Get the speed you need to get ahead.<br />
NALGENE MF75 Filters with PES Membrane<br />
Capacity<br />
mL<br />
Pore<br />
Size<br />
µm<br />
Membr.<br />
Diam.<br />
mm<br />
Fits Bottle- Number<br />
neck Size Per Case<br />
<strong>Catalogue</strong><br />
Number<br />
PES Filter Units<br />
150 0.2* 50 — 12 565-0020<br />
150 0.45 50 — 12 165-0045<br />
250 0.2* 50 — 12 568-0020<br />
250 0.45 50 — 12 168-0045<br />
500 0.2* 75 — 12 566-0020<br />
500 0.45 75 — 12 166-0045<br />
500 0.2* 90 — 12 569-0020<br />
500 0.45 90 — 12 169-0045<br />
1000 0.2* 90 — 12 567-0020<br />
1000 0.45 90 — 12 167-0045<br />
PES Bottle-Top Units<br />
150 0.2* 50 33 12 596-3320<br />
150 0.45 50 33 12 296-3345<br />
150 0.2* 50 45 12 596-4520<br />
150 0.45 50 45 12 296-4545<br />
500 0.2* 75 33 12 595-3320<br />
500 0.45 75 33 12 295-3345<br />
500 0.2* 75 45 12 595-4520<br />
500 0.45 75 45 12 295-4545<br />
1000 0.2* 90 33 12 597-3320<br />
1000 0.2* 90 45 12 597-4520<br />
* Supor machV PES membrane.
Specifications:<br />
Proteus Mini Clarification Spin Column Pack<br />
Membrane type: Hydrophilic PVDF<br />
Plastic construction: Polypropylene<br />
Pore size: 0.2 µm<br />
Maximum sample volume: 0.65 ml<br />
Hold-up volume : < 5 µl<br />
Maximum g force: 16,000 g<br />
Typical spin times: 1-2 mins for 0.65 ml sample at 14,000 g<br />
Storage: Store at RT (non-sterile)<br />
Shelf-life: 24 months<br />
Ordering Information:<br />
<strong>Generon</strong>’s Proteus mini clarification spin<br />
columns are designed to remove<br />
microorganisms, particles and precipitates<br />
larger than 0.2 µm pore size from aqueous<br />
solutions. These are ideal for HPLC/FPLC<br />
sample preparation. The PVDF membrane<br />
provides high flow rates and throughput,<br />
low extractables and broad chemical<br />
compatibility. The membrane binds far less<br />
protein than nylon, cellulose or PES<br />
membranes. The columns fit all standard<br />
microfuges and allow you to process<br />
multiple samples in parallel.<br />
Product Units Order Code Price<br />
Proteus mini clarification spin 100 GEN-MSF500 £78.00<br />
column pack
Chromatography +<br />
Electrophoresis + Dialysis<br />
Chromatography<br />
-Proteus Mini Purification Spin Columns from <strong>Generon</strong> (50 µl – 0.65 ml)<br />
-1-Step Batch Spin Columns for Batch Incubation/Centrifugation from <strong>Generon</strong> (1-20 ml)<br />
-Proteus FliQ FPLC Columns ( 1 ml, 5 ml, 10 ml bed volumes)<br />
-Single Step Columns for Batch Incubation/LC Elution from Thompson (10-600 ml)<br />
-Affinity Resin: Protein A and Protein G Sepharose, Ni-IDA Sepharose, Tetradendate Ni-<br />
Superflow, Glutathione Superflow Resin<br />
-Proteus Antibody and His-tagged Protein Purification Spin Column Kits from <strong>Generon</strong><br />
-Adenovirus and Lentivirus Purification Kits (20 ml-1000 ml cell culture volumes)<br />
-Protein Desalting (PD10 equivalent) columns from emp Biotech GmbH<br />
DNA/RNA Electrophoresis<br />
-EasyCast Horizontal Agarose Gel Tanks<br />
-Power Packs<br />
NuSep Pre-Cast Protein Gels<br />
-LongLife Tris-HEPES Protein Gels e.g. 8%, 10%, 12%, 4-20%, 8-16% Acrylamide Gels<br />
-1 year Tris-Gly Protein Gels e.g. 8%, 10%, 12%, 4-20%, 8-16% Acrylamide Gels<br />
-Accessories e.g. Running buffer, Sample loading buffer, Western transfer buffer<br />
Rapid SDS-PAGE Stain<br />
-Rapid Quick Coomassie Stain<br />
Fermentas Pre-stained and Unstained Protein Ladders and Bradford Protein<br />
Concentration Reagent Kit<br />
-Prestained and Unstained Protein Ladders<br />
-Bradford Coomassie Plus Reagent Kit from Pierce<br />
Micro-Dialysis Tubes<br />
-GeBAflex-tube (Dialysis of 10 µl-20 ml samples)<br />
Mini (10-250 µl), Midi (50-800 µl), Maxi (100-3000 µl, Mega (3 -20 ml)<br />
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Specifications:<br />
Proteus Mini Purification Spin Column Pack<br />
Sinter type: Ultra High Density Polyethylene<br />
Plastic construction: Polypropylene<br />
Pore size: 10-40 µm (mean pore size, 18 µm)<br />
Maximum reservoir volume: 0.65 ml<br />
Hold-up volume : < 5 µl<br />
Maximum g force: 14,000 g<br />
Typical spin times: 1 min for 0.65 ml sample at 14,000 g<br />
Storage: Store at RT (non-sterile)<br />
Shelf-life: 24 months<br />
Ordering Information:<br />
<strong>Generon</strong>’s Proteus mini purification spin<br />
columns are designed for small scale<br />
protein purifications such as those<br />
required for expression trials, solubility<br />
determination tests, screening, titering<br />
and scouting studies. The columns fit all<br />
standard microfuges and allow you to<br />
process multiple samples in parallel.<br />
Product Units Order Code Price<br />
Proteus mini purification spin 25 GEN-MPS500 £25.00<br />
column pack
Your Lysate<br />
Your Purified Protein<br />
Spin<br />
Proteus ‘1-step batch’ Midi Spin Column Pack<br />
Any Resin<br />
1. Remove the clear TOP spin cap and load the required amount of resin. Add 10 ml<br />
binding buffer, replace the clear TOP spin cap and spin at 200 g for 2 min to equlibrate<br />
the resin matrix.<br />
2. Carefully remove the spin column barrel and push the beige BOTTOM cap on to the end<br />
of the tapered part of the spin column. Place the spin column into a fresh 50 ml<br />
centrifuge tube.<br />
3. Remove the clear TOP spin cap, load the sample, either filtered or unfiltered, into the<br />
upper reservoir (containing the resin) and screw the yellow TOP cap tightly on to the<br />
centrifuge tube.<br />
4. Place the column on a standard tube roller and mix for a required time. Most<br />
applications require only a 30 min incubation.<br />
5. After batch incubation, replace the yellow TOP cap with the clear TOP spin cap.<br />
Carefully remove the spin column barrel from the centrifuge tube and the beige<br />
BOTTOM cap from the tapered end of the spin column. Place the spin column barrel<br />
into a 50 ml centrifuge tube and spin at 200g for 2 min. Remove the filtrate.<br />
6. Add 15 ml wash buffer. Wash off the unbound protein at 200 g for 2 min. Remove the<br />
filtrate. Repeat this step.<br />
7. Place the spin column barrel into a fresh tube. Add up to 10 ml elution buffer to the<br />
upper reservoir and elute the purified protein at 200 g for 2 min.<br />
<strong>Generon</strong>’s Proteus ‘1-step-batch’ spin columns<br />
allow you to batch mix your filtered or<br />
unclarified sample with the resin of your choice<br />
and then wash and elute your purified and<br />
concentrated protein in a small volume. These<br />
columns allow you to process multiple samples<br />
in parallel. The spin column barrels fit all 50 ml<br />
conical centrifuge tubes and can be used in<br />
standard bench centrifuges containing a swing<br />
bucket rotor.<br />
There is no resin carry-over to a subsequent downstream analyses, no messy transfer of<br />
samples/resin slurries and negligible hold-up volume. As the device is contained, it is ideal for<br />
handling biohazardous samples and samples from infectious sources.<br />
Method
Data<br />
Graph showing the typical IgG capacity of a Protein A ‘1-step batch’ spin column after 3 re-uses<br />
using unclarified rabbit serum. The serum was incubated for 20 min in batch mode on a roller and<br />
the purified IgG was then eluted using centrifugation at 200 g for 2 min.<br />
Specifications<br />
Plastic spin column barrel construction: Polypropylene<br />
Yellow TOP ‘sample incubation’ screw cap: Polypropylene<br />
Beige BOTTOM ‘sample incubation’ cap: TPE<br />
Clear TOP ‘spin’ cap: Polypropylene<br />
Mean sinter pore size: 18 µm<br />
Sinter construction: low protein-binding ultra high density polyethylene<br />
Maximum upper reservoir volume: 22 ml<br />
Typical g force: 200 g<br />
Typical spin times: 2 min<br />
Max. g force: 1,500 g<br />
Storage: Store at RT (non-sterile)<br />
Ordering Information<br />
Product Units Order Code Price<br />
Proteus ‘1-step batch’ Midi spin column pack 8 GEN-1SB08 £32.00<br />
Tel: (01753) 866-511<br />
Fax: (01753) 20-88-99<br />
E-mail: info@generon.co.uk<br />
<strong>Generon</strong> Ltd,<br />
12 Rawcliffe House, Howarth Road,<br />
Maidenhead,<br />
Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk
Specifications:<br />
Thompson Single StEP chromatography columns<br />
Thompson’s Single StEP Empty Columns allow<br />
you the opportunity to fill different sized<br />
columns with resins for protein purification<br />
applications. The sample is mixed with the resin<br />
and batch incubated. The columns can then be<br />
attached to an FPLC or peristaltic pump and the<br />
purified protein can then eluted with a<br />
gradient.<br />
Sinter type: Ultra High Density Polyethylene<br />
Plastic construction: Polypropylene<br />
Pore size: 25 µm<br />
Mode of action: Gravity or Batch Incubation/Pump Elution<br />
Reservoir volume: 10 ml, 25 ml, 50 ml, 100 ml, 200 ml, 320 ml, 650 ml<br />
Storage: Store at RT (non-sterile)<br />
Order Code Description Pack size Price<br />
9452086-10 10 ml Single StEP column with bottom frit 10 £98.00<br />
9452088-10 25 ml Single StEP column with bottom frit 10 £98.00<br />
9452090-10 50 ml Single StEP column with bottom frit 10 £108.00<br />
9452092-10 100ml Single StEP column with bottom frit 10 £148.00<br />
9452094-10 200 ml Single StEP column with bottom frit 10 £148.00<br />
9452099-10 320 ml Single StEP column with bottom frit 10 £310.00<br />
9452097-10 650 ml Single StEP column with bottom frit 10 £355.00<br />
235008-20 Dual ended Caps (Blue) 20 £25.00
Specifications:<br />
Maximum pressure 1 ml column: 5 bar (70 psi)<br />
Maximum pressure 5, 10 & 20 ml column: 3 bar (42 psi)<br />
pH stability: 2-14<br />
Recommended flow rate: 0.5-2 ml/min<br />
Chemical compatibility: All common aqueous solutions<br />
Denaturants e.g. 8 M Urea, 4 M guanidine<br />
hydrochloride and chaotropic salts.<br />
1 ml column housing: Medical grade polypropylene<br />
5 ml, 10 ml & 20 ml column housing: Acrylic<br />
Bed dimensions of the 1 ml column: 33 x 6.2 mm i.d.<br />
Bed dimensions of the 5 ml column: 52 x 11.0 mm i.d.<br />
Bed dimensions of the 10 ml column: 104 x 11.0 mm i.d.<br />
Bed dimensions of the 20 ml column: 100 x 16.0 mm i.d.<br />
End Adaptors (polypropylene): 10-32 UNF female thread (1/16”)<br />
Stop plug: 10-32 UNF male thread (1/16”)<br />
Product Quantity Order code<br />
Proteus 1 ml FliQ column<br />
Price: £15.00<br />
Proteus 5 ml FliQ column<br />
Price: £24.00<br />
Proteus 10 ml FliQ column<br />
Price: £35.00<br />
Proteus 20 ml FliQ column<br />
Price: £70.00<br />
10.32 packing connector<br />
Price: £6.50<br />
Proteus FliQ Columns<br />
<strong>Generon</strong> now offers empty 1 ml, 5 ml, 10<br />
ml and 20 ml FPLC chromatography<br />
columns at an affordable price. Both ends<br />
of the FliQ columns have 10-32 UNF<br />
threads which fits all common<br />
chromatography instruments such as AKTA.<br />
Pack your own resin into these columns.<br />
Separate your proteins using an FPLC,<br />
HPLC, low pressure pump or a syringe. Use<br />
your own resins or use our own high<br />
capacity: high flow rate resins eg Protein A<br />
Agarose, Protein G Agarose, Ni-Superflow,<br />
Glutathione Superflow resin.<br />
1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs)<br />
1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs)<br />
1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs)<br />
1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs)<br />
Luer/Thread connector for packing FliQ columns (1 pc)<br />
GEN-FliQ1<br />
GEN-FliQ5<br />
GEN-FliQ10<br />
GEN-FliQ20<br />
GEN-10.32
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Do you purify GST-tagged or His-tagged proteins?<br />
New Glutathione Superflow (SuperGlu) and tetradentate Ni-SuperFlow Resin<br />
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GENERATE BINDING CAPACITIES OF 10-20 MG/ML AT FLOW RATES UP TO 140 PSI!<br />
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Order Code Description Price (£)<br />
SuperGlu 10 Glutathione Superflow (10 ml) £120.00<br />
SuperGlu 25 Glutathione Superflow (25 ml) £275.00<br />
SuperGlu 100 Glutathione Superflow (100 ml) £900.00<br />
GEN-MCS25 Ni-Superflow Plus Resin (25 ml) £182.00<br />
GEN-MCS100 Ni-Superflow Plus Resin (100ml) £665.00<br />
GEN-MCS500 Ni-Superflow Plus Resin (500 ml) £2,450.00<br />
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Optimized for high-performance purification of<br />
GST and His-tagged proteins.<br />
Negligible ligand leakage.<br />
Typical binding capacity of 10-20 mg/ml resin.<br />
Higher flow rates than Sepharose resin.<br />
Compatible with a wide range of reducing<br />
agents, denaturants etc.<br />
Supplied in 10 ml, 25 ml, 100 ml and 500 ml<br />
quantities.<br />
<strong>Generon</strong> Ltd,<br />
12 Rawcliffe House, Howarth Road,<br />
Maidenhead,<br />
Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk<br />
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Recombinant Protein A and Protein G Sepharose FF resin for<br />
Immuno-precipitation<br />
Benefits<br />
• High Quality immobilized Protein A and G Sepharose FF resin.<br />
• Selectively optimized for your IP work. The resins are screened<br />
through a 300 µm filter to remove clumps.<br />
• High Capacity recombinant Protein A and Protein G Sepharose FF resin.<br />
<strong>Generon</strong> 2012 Prices<br />
1 ml recombinant Protein A Sepharose FF resin for £70.00 (Order code: PC-A1)<br />
1 ml recombinant Protein G Sepharose FF resin for £70.00 (Order code: PC-G1)<br />
5 ml recombinant Protein A Sepharose FF resin for £210.00 (Order code: PC-A5)<br />
5 ml recombinant Protein G Sepharose FF resin for £210.00 (Order code: PC-G5)<br />
These prices are over 50% lower than those from GE Healthcare!<br />
GE Healthcare 2009 Price Comparison<br />
5 ml Protein A Sepharose FF resin £335.00 (GE Healthcare website, 17-5138-01, 18 th January 2009)<br />
5 ml Protein G Sepharose FF resin £348.00 (GE Healthcare website, 17-0618-01, 18 th January 2009)<br />
<strong>Generon</strong> Ltd., 12 Rawcliffe House, Howarth Road, Maidenhead SL6 1AP.<br />
Tel: +44 (0) 1753 866 511; Fax: +44 (0) 1753 20-88-99<br />
E-mail: info@generon.co.uk www.generon.co.uk
Proteus NoEndo TM M (Mini), NoEndo TM S (Standard) and NoEndo TM HC<br />
(High Capacity) Spin Column Kits: The Gold Standard<br />
Residual endotoxin contamination in advanced biotherapy products is an expensive and<br />
often difficult contaminant to control.<br />
Many commercially-available protocols are unable to remove endotoxins effectively<br />
and are based on non-affinity chromatography methods e.g. Ion exchange<br />
chromatography, phase separation using Triton X-114 or require time consuming and<br />
expensive affinity steps. These costly resins are often supplied as loose resin or packed<br />
in slow gravity columns.<br />
The Proteus NoEndo TM spin column kits offer a standardised method for high grade<br />
clearance of endotoxin from recombinant proteins, antibodies and viral vectors. These<br />
agents are increasingly being designed for therapeutic applications, hence moving them<br />
forward efficiently through in vivo studies requires pure preparations of the samples.<br />
LPS molecule<br />
Next generation Proteus kits combine the quality separation you expect from gravity flow columns with the speed and<br />
ease-of-use of spin columns. Both column formats reveal a high degree of innovation! The Standard and High Capacity<br />
columns incorporate pre-packed resin cartridges utilizing our FlowGo TM technology. The Mini columns are empty<br />
columns that incorporate our proprietary SelfSeal TM membrane technology. This ensures that there is no passage of<br />
the sample through the membrane during the batch incubation at 4 0 C or at room temperature.<br />
NoEndoS and NoEndoHC Columns:<br />
The proprietary FlowGo TM technology regulates sample movement through the technologically-advanced affinity resin<br />
cartridge, increasing both endotoxin removal and protein recovery. Uniquely, we offer flow rate control for endotoxin<br />
removal in a centrifuge.<br />
NoEndoM Columns:<br />
The NoEndo TM Mini columns incorporate a SelfSeal TM membrane technology which retains the NoEndo TM resin and<br />
sample in the batch incubation chamber. When the column spun in a low speed centrifuge, the pores of the<br />
membrane dilate and the filtered eluate is collected in the bottom of the centrifuge tube.<br />
We offer three versions of the Proteus kits: Mini, Standard and High Capacity<br />
� Proteus NoEndo TM Mini: for endotoxin loads less than 3,000 EU<br />
� Proteus NoEndo TM Standard: for endotoxin loads less than 30,000 EU<br />
� Proteus NoEndo TM High Capacity: for endotoxin loads less than 1,000,000 EU<br />
Proteus NoEndo TM M, NoEndo TM S and NoEndo TM E.coli Expressions<br />
Mammalian Expressions<br />
Insect Expressions<br />
Yeast Expressions<br />
Antibody Samples<br />
Final Polishing Steps<br />
HC applications<br />
Endotoxin-free preparation in less than 1 hour<br />
The yields of a gravity flow with the speed of a spin column<br />
NoEndo TM M NoEndo TM S NoEndo TM HC<br />
www.generon.co.uk 1
Convenient<br />
For NoEndo TM S and NoEndo TM HC columns only incorporating the FlowGo TM Technology:<br />
Pre-packed chromatography resin plug – no mess, no filling columns, no pumps,<br />
no lengthy steps and minimal optimisation required.<br />
For NoEndo TM M columns, incorporating the SelfSeal TM Technology: Minimal manual<br />
intervention, high capture efficiency, no dilution of sample, perfect for final polishing steps.<br />
Easy-to-use Full technical and application handbook including unambiguous protocols supplied with<br />
every kit.<br />
Rapid Endotoxin removal and high protein recovery (typically >90%) in 30 min for NoEndo TM S and<br />
NoEndo TM HC columns. For low endotoxin loads, use NoEndo Mini columns. Typically,<br />
Protein Recovery (%)<br />
Protein Recovery (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
Product performance<br />
0<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
NoEndo<br />
Rabbit Immunoglobulin G Bovine Serum Albumin<br />
TM HC<br />
100<br />
540000<br />
98 97<br />
6000<br />
Sample Post 1st<br />
column<br />
15<br />
Post 2nd<br />
column<br />
100 98 98<br />
3500<br />
80<br />
Recovery (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
Figure 3: The Proteus NoEndo TM S and Proteus NoEndo TM HC spin columns exhibit low protein binding. The data<br />
represents a wide range of proteins that were used through the column regardless of their iso-electric point (pI).<br />
Typical protein recoveries close to 90% were obtained.<br />
When to use Proteus NoEndo TM Mini, NoEndo TM Standard or NoEndo TM High Capacity spin columns?<br />
The Proteus NoEndo TM columns have different endotoxin binding capacities. For samples with endotoxin loads less<br />
than 3,000 EU, Proteus NoEndo TM M columns are ideal. For samples with endotoxin loads less than 30,000 EU, Proteus<br />
NoEndo TM S can be used. For samples with endotoxin loads less than 1,000,000 EU, Proteus NoEndo TM HC columns are<br />
required.<br />
Column Specifications<br />
Spin Columns NoEndo M NoEndo S NoEndo HC<br />
Typical in situ binding capacity per column 3,000 EU 30,000 EU 500,000-1,000,000 EU<br />
Typical endotoxin binding capacity 300 EU/ml 1,500 EU/ml 30,000 EU/ml<br />
Minimum endotoxin levels tested post-column
Ordering Information<br />
Description Product Code Quantity<br />
Kits<br />
Proteus NoEndoM 2 column kit (2 x 0.25 ml resin vials)<br />
Proteus NoEndoS 2 column kit<br />
Proteus NoEndoHC 2 column kit<br />
Contents – 2 spin columns, 2 centrifuge tubes,<br />
2 resin cartridges/vials<br />
Proteus NoEndoM 12 column kit (12 x 0.25 ml resin vials)<br />
Proteus NoEndoS 12 column kit<br />
Proteus NoEndoHC 12 column kit<br />
Contents – 12 spin columns, 12 centrifuge tubes,<br />
12 resin cartridges/vials<br />
Proteus NoEndoM 48 column kit (48 x 0.25 ml resin vials)<br />
Proteus NoEndoS 48 column kit<br />
Proteus NoEndoHC 48 column kit<br />
Contents – 48 Midi spin columns, 48 resin<br />
cartridges/vials (excludes 50 ml centrifuge tubes)<br />
Loose Resin<br />
GEN-NoE2M<br />
GEN-NoE2S<br />
GEN-NoE2HC<br />
GEN-NoE12M<br />
GEN-NoE12S<br />
GEN-NoE12HC<br />
GEN-NoE48M<br />
GEN-NoE48S<br />
GEN-NoE48HC<br />
2 M spin columns<br />
2 S spin columns<br />
2 HC spin columns<br />
12 M spin columns<br />
12 S spin columns<br />
12 HC spin columns<br />
48 M Spin Columns<br />
48 S spin columns<br />
48 HC spin columns<br />
NoEndo TM Resin (25 ml loose resin) GEN-NoE025ML 25 mL NoEndo TM resin<br />
NoEndo TM Resin (100 ml loose resin)<br />
GEN-NoE100ML 100 mL NoEndo TM resin<br />
Accessories<br />
Empty spin/batch columns<br />
Proteus ‘1-step batch’ Midi spin column pack<br />
Empty FPLC columns<br />
GEN-1SB08<br />
www.generon.co.uk 5<br />
8 pack<br />
Proteus 1 ml FliQ column<br />
GEN-FliQ1<br />
1 column<br />
Proteus 5 ml FliQ column GEN-FliQ5 1 column<br />
Proteus 10 ml FliQ column GEN-FliQ10 1 column<br />
Proteus 20 ml FliQ column GEN-FliQ20 1 column<br />
Empty scalable columns<br />
10 ml Single step column with bottom frit<br />
25 ml Single step column with bottom frit<br />
50 ml Single step column with bottom frit<br />
100 ml Single step column with bottom frit<br />
Ultrafiltration concentrators<br />
Vivaspin 20 (10 kDa MWCO, 12 pc)<br />
Vivaspin 20 (10 kDa MWCO, 48 pc)<br />
9452086-10<br />
9452088-10<br />
9452090-10<br />
9452092-10<br />
VS2001<br />
VS2002<br />
10 pack<br />
10 pack<br />
10 pack<br />
10 pack<br />
12 pack<br />
48 pack<br />
<strong>Generon</strong> Ltd, 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire SL6 1AP, UK<br />
Tel : +44 (0)1753 866-511 Fax : +44 (0)1753 20-88-99 Email : info@generon.co.uk
MINI<br />
MIDI<br />
Binding Affinities of<br />
Protein A & Protein G<br />
Protein A Protein G<br />
Human IgG1 ✔✔✔✔ ✔✔✔✔<br />
Human IgG2 ✔✔✔✔ ✔✔✔✔<br />
Human IgG3 ✖ ✔✔✔✔<br />
Human IgG4 ✔✔✔✔ ✔✔✔✔<br />
Human IgA ✔✔ ✖<br />
Human IgD ✔✔ ✖<br />
Human IgE ✔✔ ✖<br />
Human IgM ✔✔ ✖<br />
Mouse IgG1 ✔ ✔✔<br />
Mouse IgG2a ✔✔✔✔ ✔✔✔✔<br />
Mouse IgG2b ✔✔✔ ✔✔✔<br />
Mouse IgG3 ✔✔ ✔✔✔<br />
Rat IgG1 ✖ ✔<br />
Rat IgG2a ✖ ✔✔✔✔<br />
Rat IgG2b ✖ ✔✔<br />
Rat IgG2c ✔ ✔✔<br />
✖ ✔✔✔✔<br />
No affinity Strong affinity<br />
PROTEUS<br />
Antibody Purification<br />
Proteus A & G kits are<br />
designed for simple, rapid and<br />
multiple antibody purification<br />
from serum, ascites and tissue<br />
culture supernatant derived<br />
from static cultures and<br />
bioreactors.<br />
Proteus purification kits<br />
incorporate convenient, ready<br />
to use, single step affinity spin<br />
columns, offering the<br />
researcher total protein<br />
purification solutions.<br />
Convenient and<br />
easy-to-use<br />
• Simply insert the pre-packed<br />
chromatography resin plug into the<br />
device and place it in the centrifuge.<br />
• No mess, no filling columns, no<br />
attachment of accessories, no pumps<br />
and no lengthy equilibration.<br />
• Kits are supplied with a<br />
comprehensive technical and<br />
application-supported handbook, a<br />
step-wise laminated protocol card<br />
and all necessary buffers for rapid<br />
and simple antibody purification.<br />
Rapid<br />
• The purification protocols provided<br />
in the operating handbook permit<br />
fast recovery of pure antibodies.<br />
• Multiple samples can be processed<br />
at the same time, allowing rapid<br />
screening for antibody production.<br />
Cost-effective<br />
• Proteus spin columns can be used by<br />
any laboratory with access<br />
to a centrifuge.<br />
• The Proteus columns are re-usable.<br />
Each Mini spin column can be used<br />
up to 3 times and each Midi spin<br />
column can be used up to 5 times.<br />
• As research budgets are always under<br />
pressure, Proteus kits offer a<br />
cost-effective alternative.<br />
High Performance<br />
• Proteus chromatography spin<br />
columns will typically bind the target<br />
antibody with greater than 90%<br />
recovery.<br />
• Choice of both Protein A & G for<br />
complete coverage of antibody<br />
subclasses and host species.<br />
• Antibodies are sufficiently pure for<br />
radiolabelling, conjugation or<br />
preparation of immuno-affinity<br />
columns, ELISA and Western blots.<br />
A new and innovative range of<br />
high performance agarose-based<br />
antibody purification kits that use<br />
your laboratory centrifuge
M I N I<br />
Plug and Play Design<br />
Recessed end<br />
Place the plug into the spin column<br />
with the recessed end uppermost.<br />
Push the plug FULLY into the<br />
tapered end of the spin column<br />
using the plug insertion tool.<br />
• Complete purification in less than 20 minutes • Low % CV<br />
• Kits include enough ready-to-use buffers for 48 mini preps<br />
The Mini Protein A & G spin column is the ideal tool for screening antibody<br />
expression and for small-scale purification of antibodies for solution-state immunoassays,<br />
immuno-histochemical and immuno-fluorescence studies, Western blotting<br />
and immuno-precipitation studies. Antibodies are purified using a powerful,<br />
patented affinity spin column using a microfuge common to all biochemistry and<br />
immunology laboratories.<br />
MINI SPECIFICATIONS<br />
Kit PROTEIN A PROTEIN G<br />
Source Recombinant Protein A expressed in Recombinant Protein G expressed in<br />
E. coli (N.B. No toxic bacterial E. coli (N.B. The recombinant Protein G<br />
contaminants normally found in lacks the albumin-binding domain found<br />
native Protein A) in native Protein G)<br />
Binding capacity per use Typically 1 mg human IgG Typically 1 mg human IgG<br />
Typical number of uses per plug 3 3<br />
Supporting Proteus matrix Covalently coupled to agarose resin Covalently coupled to agarose resin<br />
Resin bed volume 0.23 ml 0.23 ml<br />
Ligand density 3.5 mg Protein A/ml resin 2 mg Protein G/ml resin<br />
Bead size range 60-165 µm 45-165 µm<br />
Maximum volume 0.65 ml 0.65 ml<br />
Storage temp. for resin plugs 2-8 ˚C 2-8 ˚C<br />
Recommended working pH pH 2.5-9.0 pH 2.0-9.0<br />
Plastic construction Polypropylene Polypropylene<br />
Color coded end-caps Red Yellow<br />
MINI PERFORMANCE<br />
205 kD<br />
116 kD<br />
97 kD<br />
66 kD<br />
45 kD<br />
Albumin<br />
31 kD<br />
The Mini spin<br />
column is now<br />
21.5 kD<br />
ready for preequilibration<br />
with<br />
14.4 kD<br />
binding buffer. Marker Sample Final Eluate<br />
Reducing SDS-Polyacrylamide gel of human IgG purified<br />
from serum with the Proteus Protein A kit<br />
Recovered Antibody (mg)<br />
1.6<br />
1.4<br />
1.2<br />
1<br />
0.8<br />
0.6<br />
0.4<br />
0.2<br />
0<br />
1.32<br />
1.38<br />
1.26<br />
1 2 3<br />
Number of Re-uses<br />
Heavy chain<br />
Light chain<br />
DIMENSIONS (mm)<br />
31<br />
10<br />
8<br />
14<br />
10<br />
Graph showing that typical<br />
IgG capacity of Protein A<br />
Mini spin columns remain<br />
unaffected after 3 re-uses<br />
using rabbit serum.<br />
Low % CV.
M I D I<br />
Plug and Play Design<br />
Recessed end<br />
Place the plug into the spin column<br />
with the recessed end uppermost.<br />
Push the plug FULLY into the<br />
tapered end of the spin column<br />
using the plug insertion tool.<br />
The Midi spin<br />
column is now<br />
ready for preequilibration<br />
with<br />
binding buffer.<br />
After use, the<br />
plug is<br />
removed using<br />
the plug<br />
insertion tool.<br />
• Complete purification in less than 60 minutes • High yields of functional antibodies<br />
• Concentrated antibodies purified in small elution volumes<br />
The Midi Protein A & G spin column permits semi-preparative purification of<br />
concentrated monoclonal and polyclonal antibodies for all downstream applications.<br />
Both the Mini and Midi kits contain all the resin spin columns, buffers and<br />
ultrafiltration spinners necessary for rapid and convenient purifications of your<br />
target antibodies.<br />
MIDI SPECIFICATIONS<br />
Kit PROTEIN A PROTEIN G<br />
Source Recombinant Protein A expressed in Recombinant Protein G expressed in<br />
E. coli (N.B. No toxic bacterial E. coli (N.B. The recombinant Protein G<br />
contaminants normally found in lacks the albumin-binding domain found<br />
native Protein A) in native Protein G)<br />
Binding capacity per use Typically 20 mg human IgG Typically 20 mg human IgG<br />
Typical number of uses per plug 5 5<br />
Supporting Proteus matrix Covalently coupled to agarose resin Covalently coupled to agarose resin<br />
Resin bed volume 1.6 ml 1.6 ml<br />
Ligand density 3.5 mg Protein A/ml resin 2 mg Protein G/ml resin<br />
Bead size range 60-165 µm 45-165 µm<br />
Maximum volume 20 ml 20 ml<br />
Storage temp. for resin plugs 2-8 ˚C 2-8 ˚C<br />
Recommended working pH pH 2.5-9.0 pH 2.0-9.0<br />
Plastic construction Polypropylene Polypropylene<br />
Color coded end-caps Red Yellow<br />
MIDI PERFORMANCE<br />
205 kD<br />
116 kD<br />
97 kD<br />
66 kD<br />
Albumin Heavy chain<br />
45 kD<br />
Recovered Antibody (mg)<br />
31 kD<br />
21.5 kD<br />
Marker Sample Final Eluate<br />
Reducing SDS-Polyacrylamide gel of mouse IgG1 purified<br />
from cell culture supernatant (+ foetal calf serum) with<br />
the Proteus Protein G kit<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
32.7<br />
31.5<br />
Light chain<br />
34.0 32.9 32.5<br />
1 2 3 4 5<br />
Number of Re-uses<br />
DIMENSIONS (mm)<br />
76<br />
25<br />
12<br />
29<br />
25<br />
Graph showing that typical IgG<br />
capacity of Protein A Midi<br />
spin columns remain<br />
unaffected after 5 re-uses<br />
using rabbit serum.<br />
Low % CV.
PROTEUS KIT CONTENTS<br />
Centrifuge<br />
Tubes x 32<br />
Plug Insertion Tool x 1<br />
Ultrafiltration Spinners x 16<br />
Spin Columns x 16<br />
Resin Plugs x 16<br />
30 ml Neutralization Buffer C<br />
Mini kits MiDi kits<br />
ORDERING INForMATION<br />
Mini Products Quantity Order codes<br />
Mini Protein A & G Starter Pack 1 PC-MAG04<br />
(2 A & 2 G Spin Columns)<br />
Mini Protein A Kit (16 units) 1 PC-MAK16<br />
Mini Protein G Kit (16 units) 1 PC-MGK16<br />
Mini Protein A Bulk Pack (48 units) 1 PC-MAC48<br />
Mini Protein G Bulk Pack (48 units) 1 PC-MGC48<br />
Ultrafiltration Spinners x 4<br />
Plug Insertion Tool x 1<br />
Spin Columns x 4<br />
125 ml Elution Buffer B2<br />
250 ml Binding Buffer A 125 ml Elution Buffer B1 (Protein A kits only)<br />
Resin Plugs x 4<br />
Midi Products Quantity Order codes<br />
Midi Protein A Kit (4 units) 1 PC-AK04<br />
Midi Protein G Kit (4 units) 1 PC-GK04<br />
Midi Protein A Bulk Pack (12 units) 1 PC-AC12<br />
Midi Protein G Bulk Pack (12 units) 1 PC-GC12<br />
Mini and Midi Accessories Quantity Order codes<br />
Protein A Buffer Pack 1 PC-BAP<br />
Protein G Buffer Pack 1 PC-BGP<br />
Centrifuge Tubes x 8
MINI<br />
MIDI<br />
PROTEUS<br />
Recombinant Protein Purification<br />
REFINE the way you purify your proteins REPLACE your conventional spin columns REDUCE your hands-on time and costs<br />
IMAC PERFORMANCE<br />
SDS-polyacrylamide gel showing the purity of a<br />
6xHis-tagged recombinant protein from E. coli.<br />
Marker<br />
Cell lysate<br />
1st Eluate<br />
2nd Eluate<br />
Proteus Metal Chelate Mini and Midi kits<br />
are designed for simple, complete and rapid<br />
6xHis-tagged recombinant protein<br />
purification from bacterial cells, insect<br />
vectors, mammalian cells and yeast under<br />
native or denaturing conditions.<br />
Proteus purification kits incorporate<br />
patented, single step and affinity spin<br />
columns, clear and unambiguous<br />
procedures, desalting/buffer exchange<br />
columns and ready-to-use buffers.<br />
Proteus Metal Chelate kits are ideal for<br />
screening expression clones for high levels<br />
of 6xHis-tagged proteins, scouting mutant<br />
clones for recombinant protein expression<br />
and multiple small-scale and preparative<br />
purification of 6xHis-tagged proteins for<br />
activity and structural studies.<br />
Convenient & easy-to-use<br />
• Simply insert the pre-packed<br />
chromatography resin plug into the<br />
device and place it in the centrifuge.<br />
• No mess, no filling columns, no<br />
attachment of accessories, no pumps<br />
and no lengthy equilibration.<br />
• Kits are supplied with a comprehensive<br />
technical and application-supported<br />
handbook, a step-wise laminated<br />
protocol card and all necessary buffers<br />
for rapid and simple recombinant protein<br />
purification.<br />
IMAC SPECIFICATIONS<br />
Rapid<br />
• The purification protocols provided in<br />
the operating handbook permit fast<br />
recovery of pure recombinant protein.<br />
• Multiple samples can be processed at<br />
the same time, allowing rapid<br />
screening for 6xHis-tagged proteins.<br />
• No hold-up volume or spluttering. High<br />
recovery during the fast elution step.<br />
Cost-effective<br />
• Proteus spin columns can be used by any<br />
laboratory with access to a centrifuge.<br />
• The Proteus columns are re-usable. Each<br />
Mini spin column can be used up to a<br />
minimum of 2 times and each Midi spin<br />
column can be used up to a minimum<br />
of 2 times without nickel re-charging.<br />
• As research budgets are always under<br />
pressure, Proteus kits offer a<br />
cost-effective alternative.<br />
Performance<br />
• Proteus chromatography spin columns<br />
will typically bind the target recombinant<br />
protein with greater than 90% recovery.<br />
• Proteus spin columns can purify and<br />
concentrate the protein of interest in a<br />
single step and with high reproducibility<br />
(low % CV).<br />
• Proteins are sufficiently pure for<br />
structural and activity-based studies or<br />
for raising antibodies.<br />
Kit MINI MIDI<br />
Max. sample volume per load 0.65 ml, fixed angle rotor 20 ml, swing bucket rotor<br />
Charged metal ion Ni 2+ Ni 2+<br />
Typical binding capacity per use 1 mg 6xHis-tagged protein 10 mg 6xHis-tagged protein<br />
Min. number of uses per plug 2 (assuming no further Ni 2+ charging) 2 (assuming no further Ni 2+ charging)<br />
Supporting Proteus matrix Covalently coupled to agarose resin Covalently coupled to agarose resin<br />
Resin bed volume 0.23 ml 1.6 ml<br />
Bead size range 45-165 µm 45-165 µm<br />
Storage temp. for resin plugs 2-8 ˚C 2-8 ˚C<br />
Recommended working pH pH 2.0-12.0 pH 2.0-12.0<br />
Chemical stability High High<br />
Plastic construction Polypropylene Polypropylene<br />
Time to purity 15 min 48 min<br />
Color coded end-caps Black Black
PROTEUS KIT CONTENTS<br />
Centrifuge Tubes x 24<br />
Plug Insertion Tool x 1<br />
Ultrafiltration Spinners x 24<br />
Spin Columns x 24<br />
Spin Column Plugs x 24<br />
Spin Columns x 8<br />
Mini kits MiDi kits<br />
250 ml 5 x PBS Buffer A<br />
Ultrafiltration Spinners x 8<br />
Plug Insertion Tool x 1<br />
APPLICATION DRIVERS FOR METAL CHELATE CHROMATOGRAPHY<br />
Centrifuge Tubes x 12<br />
Spin Column Plugs x 8<br />
150 ml 1 x Imidazole Buffer B<br />
Purification of recombinant proteins for activity and/or structural studies Mini & Midi Kits<br />
Purification of recombinant proteins for raising antibodies Mini & Midi Kits<br />
Multiple and parallel semi-preparative purification of 6xHis-tagged proteins Midi Kit<br />
Small-scale purification of 6xHis-tagged proteins Mini Kit<br />
Screening expression clones for high levels of 6xHis-tagged proteins Mini Kit<br />
Scouting mutant clones for recombinant protein expression Mini Kit<br />
ORDERING INForMATION<br />
Min. number of<br />
Mini Products Quantity purifications Order codes<br />
Mini IMAC Starter Kit (8 units) 1 16 PC-MMCK-08<br />
Mini IMAC Kit (24 units) 1 48 PC-MMCK-24<br />
Mini IMAC Pack (24 units; excludes UF concentrators) 1 48 PC-MMCC-24<br />
Mini IMAC Bulk Pack (72 units; excludes UF concentrators and buffers) 1 144 PC-MMCC-72<br />
Midi Products<br />
Midi IMAC Kit (8 units) 1 16 PC-MCK-08<br />
Midi IMAC Pack (8 units; excludes UF concentrators) 1 16 PC-MCC-08<br />
Midi IMAC Bulk Pack (24 units; excludes UF concentrators and buffers) 1 48 PC-MCC-24<br />
Accessories<br />
IMAC Buffer Pack (250 ml 5 x PBS Buffer A, 150 ml 1 x Imidazole Buffer B) 1 - PC-BMCP
Technical Data and Operating Instructions:<br />
The Peristaltic Pump Collar<br />
(Optional Accessories)<br />
Large volumes of dilute and particle-laden samples can be loaded on to Proteus spin columns using the peristaltic pump<br />
collar. The peristaltic pump collar (Fig. 1) locks the Proteus sealing cap securely to the spin column barrel to ensure that<br />
high sample loading flow rates can be used (Fig. 2).<br />
Materials Included:<br />
Peristaltic Pump Collar: Black Acetal<br />
Peristaltic Pump Tubing: Nalgene 180 tubing (L/S 16, 4 ft). Tubing is configured for 0.8 ml/rev. For example<br />
when taking into account the back pressure exerted by the resin column, 10-20 rpm<br />
would provide a flow rate of approximately 5-8 ml/min.<br />
Required equipment:<br />
Peristaltic pump: Variable speed peristaltic pump capable of speeds less than 20 rpm e.g. Masterflex L/S<br />
pump.<br />
Peristaltic pump head: Pump head designed for L/S 16 tubing E.g. Masterflex L/S Easy-Load pump head for<br />
precision tubing. Pump head can accommodate L/S 13, L/S 14, L/S 16, L/S 25, L/S 17,<br />
and L/S 18 precision tubing.<br />
Operation:<br />
Fig. 1<br />
Load the correct size tubing in to the peristaltic pump head. (For optimum tubing life, keep the tubing straight where it<br />
exits and enters the pump)<br />
Insert the white fittings on to the end of the peristaltic pump tubing. Connect one end of the tubing tightly to the barb<br />
connector on the sealing cap. Place the other end of the tubing into the filtered sample reservoir.<br />
Fill the column barrel with 18 ml filtered sample. Push the sealing cap fully into the top of the column barrel and then<br />
slide the peristaltic pump collar on to the sealing cap. Turn the collar (a near ¼ turn) until one of its legs slides under the<br />
hinge of the column barrel lid (Fig. 2). Ensure that the collar is positioned over the top rim of the column barrel.<br />
Run the peristaltic pump at the desired flow rates noting that 10-20 rpm (using the Type 16 tubing) is equivalent to flow<br />
rates of approximately 5-8 ml/min.<br />
The Proteus spin column can be run in peristaltic mode only. All you need to purchase is the peristaltic pump collar,<br />
sample loading cap and peristaltic pump tubing configured in cat no. PC-PPCSC (Fig. 2).
Peristaltic pump mode (for use with spin columns):<br />
Description Cat. No. Quantity Contents<br />
Fig. 2<br />
Pump Collar and Loading Cap PC-PPCSC 1 Peristaltic Pump Collar, Sample Loading<br />
Cap, Nalgene 180 (Type 16) PVC Tubing
Catalog No. CP-0108<br />
PROTEIN PURIFICATION<br />
Centri•Pure P25<br />
Hydrated Gel Filtration Columns<br />
for protein purification and desalting<br />
Processes Sample Volumes of 2.5 mL<br />
www.empbiotech.com<br />
Centri•Pure P25 Gel Filtration Columns<br />
are designed for rapid and efficient<br />
removal of small molecules (salts, dyes,<br />
ammonia, haptens, biotin, etc.) from<br />
antibodies, enzymes and other proteins.<br />
The gel matrix of Centri•Pure is<br />
Zetadex�25, a beaded composite<br />
material developed by emp BIOTECH<br />
and composed partially of polymerized<br />
dextran. It exhibits high selectivity, high<br />
resolution and chemical stability.<br />
Molecules purified with Zetadex�25 are<br />
separated according to size. Smaller<br />
molecules pass significantly slower<br />
through the column than larger<br />
molecules. Buffer and pH effects on resolution are minimal.<br />
The size exclusion cut�off for Zetadex�25 is 10 kD for proteins. Proteins larger than<br />
10 kD in a sample volume of up to 2.5 mL can be purified with an elution volume of<br />
2.7 to 3.5 mL.<br />
emp Biotech GmbH<br />
Robert-Roessle-Str. 10<br />
13125 Berlin, Germany<br />
Tel. +49 (30) 9489 2201<br />
Fax +49 (30) 9489 3201<br />
info@empbiotech.com
Catalog No. CP-0108<br />
PROTEIN PURIFICATION<br />
Four Step Protocol<br />
1. Column Preparation<br />
Remove the cap from the top and then the<br />
bottom cap of the Centri•Pure P25 Column.<br />
Allow excess column fluid to drain (via gravity)<br />
into a suitable waste reservoir.<br />
3. Sample Application<br />
Transfer 2.5 mL sample to the Centri•Pure P25<br />
Column. Allow the sample to enter the gel bed<br />
completely.<br />
High Performance Results:<br />
1,25<br />
1,00<br />
Absorption<br />
0,75<br />
0,50<br />
0,25<br />
0,00<br />
0 1 2 3 4 5 6 7 8 9 10 11 12 13<br />
Elution volume [mL]<br />
www.empbiotech.com<br />
2012-02-29 CP P25 CP-0108.doc<br />
Conductivity<br />
Fig.1: Desalting of protein solution (2.5 mg<br />
albumin (OvA) in 2.5 ml 0.8 M NaCl), elution<br />
with water<br />
2. Column Equilibration<br />
Equilibrate the column with approximately<br />
25 mL of the required buffer (use the same<br />
buffer for both equilibration and elution).<br />
Allow the equilibration buffer to enter the<br />
gel bed completely...<br />
4. Elution<br />
Place a tube for sample collection under the<br />
Centri•Pure P25 Column. Transfer 3.5 mL of<br />
elution buffer to the column and elute the<br />
purified sample.<br />
1,25<br />
1,00<br />
Absorption<br />
0,75<br />
0,50<br />
0,25<br />
0,00<br />
emp Biotech GmbH<br />
Robert-Roessle-Str. 10<br />
13125 Berlin, Germany<br />
0 2 4 6 8 10 12 14 16 18<br />
Elution volume [mL]<br />
Fig.2: Elution profile overlay of albumin (2.5 mg OvA, black<br />
line � 280 nm) and free dye (1.25 µmol FAM, orange<br />
line � 490 nm) in DMSO/NaHCO 3, elution with water<br />
Tel. +49 (30) 9489 2201<br />
Fax +49 (30) 9489 3201<br />
info@empbiotech.com
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NuSep Gels: The most affordable pre-cast polyacrylamide gels from <strong>Generon</strong><br />
2 minute UV visualisation<br />
All nUView precast gels now incorporate the<br />
unique formulation allowing protein bands to be<br />
visualised in only 2 minutes in the presence of<br />
ultraviolet light (UV).<br />
Simply place your gel over a 250-320 nm UV<br />
transilluminator and watch high-resolution bands<br />
appear in 2 minutes – taking you from analysis to<br />
results faster than any other gel on the market!<br />
With nUView technology you no longer have to<br />
stain and de-stain your gels – increasing protein<br />
recovery and saving you significant time while<br />
receiving improved results.<br />
The most affordable pre-cast<br />
polyacrylamide gels<br />
With prices up to 50% lower than Bio-Rad<br />
or Invitrogen NuSep gels are the most<br />
affordable pre-cast gels available.<br />
Don’t spend money on new<br />
equipment.<br />
Compatible with standard (10cm) mini-gel<br />
tanks including Bio-Rad and Invitrogen. An<br />
adaptor may be required for Invitrogen<br />
Sure-Lock X-Cell tanks, just ask your<br />
<strong>Generon</strong> representative.<br />
Better<br />
• Visualise protein bands in just 2 minutes under<br />
UV light. No staining required!<br />
• Long shelf life up to 2 years from date of<br />
manufacture.<br />
• Better resolution across a wider range (from<br />
205 kDa to 2.5 kDa).<br />
• Cassette design eliminates cross sample<br />
contamination and gel damage.<br />
• The most affordable pre-cast gels available with<br />
prices up to 50% lower than Bio-Rad or<br />
Invitrogen.<br />
Faster<br />
• Fast run times: 30 minutes at 250V, 45 minutes<br />
at 200V.<br />
• Easy to open cassette - no tools required.<br />
• Also compatible with MES and MOPS<br />
running buffers.<br />
Simpler<br />
• Sized to fit all (10 cm) mini-gel tanks.<br />
• Large sample well capacity (up to 50 μL)<br />
• Numbered lanes for easy well<br />
identification.<br />
Try a nUView gel today and experience the difference.
Improve your Protein Electrophoresis today<br />
nUView Tris Glycine gels provide better resolution across a wider range, from 205 kDa to 2.5 kDa.<br />
We can provide the right gel for your sample. For low molecular weight a 16% gel with Tris Tricine<br />
buffer will improve resolution of peptides and small proteins. Separate high molecular weight<br />
proteins with an 8% gel.<br />
Migration table<br />
Gel Percentage<br />
<strong>Generon</strong> Ltd., 12 Rawcliffe House, Howarth Rd, Maidenhead, Berkshire SL6 1AP<br />
nUView<br />
Precast Gels<br />
Let NuSep nUView help you rethink<br />
and improve your workflow. Save<br />
time and get better results faster<br />
• nUView 2 min visualisation to keep your<br />
work flow moving<br />
• 24 month shelf life from date of manufacture<br />
for consistent performance<br />
• 30-45 minute run time provides quick results<br />
• Use standard Tris Glycine buffer<br />
• Transfers efficiently to nitrocellulose or<br />
PVDF in under 90 min<br />
• Large well volumes (up to 50 µL) hold up to<br />
twice the volume of Invitrogen gels.<br />
• Excise bands for in-gel digestion<br />
Tel: (01753) 866-511 Fax: (01753) 20-88-99 E-mail: info@generon.co.uk www.generon.co.uk
NuSep nUView Tris-Glycine (TG) precast gels offer improved resolution and<br />
increased mass range<br />
NuSep’s nUView Tris-Glycine (TG) gels are based on<br />
a reformulation of our popular iGel. nUView TG gels<br />
offer improved resolution, broader mass range,<br />
decreased run time and increased stability.<br />
Separations using nUView TG gels can be carried out<br />
under native or denaturing conditions using a broad<br />
array of popular buffer systems. In this study we<br />
compare the resolution and migration patterns of<br />
nUView TG gels with the Bio-Rad Mini-PROTEAN®<br />
TGX TM and Invitrogen NuPAGE precast gels.<br />
Comparison of migration patterns<br />
nUView TG gels come in a variety of well<br />
configurations and acrylamide formulations (Figure 1).<br />
One of the most popular formulations is the 4-20%<br />
because it offers a broad molecular weight range and<br />
separation characteristics suitable for a wide range of<br />
proteins. We first compared the migration patterns<br />
between the nUView Tris-Glycine, Bio-Rad Mini-<br />
PROTEAN TGX and Invitrogen NuPage precast gels<br />
using the Invitrogen Mark 12 protein standards (Figure<br />
2). Excellent separation performance is achieved with<br />
the nUView TG gels when compared to the TGX and<br />
NuPAGE gels. At the lower MW region (4-2kDA)<br />
nUView TG gels achieve higher resolution than both<br />
the TGX and NuPAGE gels with both insulin chains<br />
completely resolved. Furthermore separation with<br />
nUView TG is achieved across the entire length of the<br />
gel providing a broader MW range from 200kDA to<br />
2kDa.<br />
Figure 2. Migration pattern of protein standard between<br />
nUView TG, Bio-Rad TGX and Invitrogen NuPAGE gels.<br />
MW markers were: 200, 116, 97, 66, 55, 33, 31, 21, 14, 6, 3.5, 2.5<br />
kDa. From left to right, nUView TG 4-20%, Bio-Rad TGX 4-20%,<br />
Invitrogen NuPAGE 4-12%. Seperation over a broader MW range<br />
is achieved with nUView TG gels.<br />
Comparison of resolution<br />
Gel selection is dependent not only on MW range but on<br />
achievable resolution for the sample of interest. To evaluate<br />
the resolution of nUView TG gels a variety of protein<br />
samples were subjected to SDS page (Figure 4).<br />
Figure 4. Resolution for complex protein samples on the<br />
nuView TG gel. Lanes 1-6) Invitrogen Mark 12 protein MW<br />
markers. 2-3) Gallus Gallus Egg white. 4-5) Pisum sativum (English<br />
pea) protein extract. 7-8) Gallus Gallus Egg white dilute. 9-10)<br />
Escherichia coli lysate. 11-12) Human plasma.<br />
Resolution was then compared between the nUView TG,<br />
Bio-Rad TGX, and Invitrogen NuPAGE using the SDS page<br />
analysis of Pisum sativum extract. Comparison of the<br />
banding pattern indicated an increased resolution for the<br />
nUView TG gels, evident by the increase in protein band<br />
separation and number (Figure 5).<br />
Figure 5. Comparison of resolution between nUView TG,<br />
Bio-Rad TGX and Invitrogen NuPAGE. Pisum sativum (English<br />
pea) protein extract was separated and the number of protein<br />
bands and spacing between bands was compared. Left) full gel<br />
lane comparison. Right) The region between 55 and 31 kDa<br />
indicating increased banding and higher resolution for the<br />
nUView TG gel.
Gel Specifications: NuSep Tris Glycine Max nUView gels (Invitrogen Size Cassettes)<br />
Cassette Size: 10 x 10 x 0.7 cm Gel Size: 7.5 x 8.0 x 0.1 cm<br />
Running Buffer: Tris Glycine SDS Shelf-Life: 24 months at 4°C<br />
Sample Buffer: Tris HCl SDS<br />
Voltage: 200-250 V<br />
Run Time: 45 min in Tris Gly buffer<br />
Ordering information for NuSep Max gels (For Invitrogen Mini Tanks Only)<br />
Tris Gly Max<br />
nUView<br />
(pack of 10 gels)<br />
% Acrylamide 10 well (45 µL) 12 well (25 µL) 17 well (15 µL) Price<br />
8% NN10-008 NN12-008 NN17-008 £ 59.95<br />
10% NN10-010 NN12-010 NN17-010 £ 59.95<br />
12% NN10-012 NN12-012 NN17-012 £ 59.95<br />
8-16% NN10-816 NN12-816 NN17-816 £ 59.95<br />
4-20% NN10-420 NN12-420 NN17-420 £ 59.95<br />
Gel Specifications: NuSep Tris Glycine Universal nUView gels (For Bio-Rad Mini Protean<br />
II and III, Invitrogen XCell I and II and Surelock and Hoefer Tall Mighty Small and Hoefer<br />
Mighty Small Tanks)<br />
Universal<br />
Universal<br />
Cassette Size: 10 x 8.5 x 0.44 cm<br />
Gel Size: 8 x 5.8 x 0.1 cm<br />
Running Buffer: Tris Glycine SDS Shelf-Life: 24 months at 4°C<br />
Voltage: 200 V Sample Buffer: Tris HCl SDS<br />
Run Time: 45 min<br />
Tris Glycine<br />
nUView<br />
Universal<br />
cassette<br />
(pack of 10 gels)<br />
Accessories<br />
New Larger Invitrogen-size Max Gels<br />
% Acrylamide 10 well (50 µL) 12 well (30 µL) 15 well (25 µL) Price<br />
8% NG21-008 NG11-008 NG31-008 £ 59.95<br />
10% NG21-010 NG11-010 NG31-010 £ 59.95<br />
12% NG21-012 NG11-012 NG31-012 £ 59.95<br />
8-16% NG21-816 NG11-816 NG31-816 £ 59.95<br />
4-20% NG21-420 NG11-420 NG31-420 £ 59.95<br />
Description Product code Price<br />
Tris HEPES running buffer 5L sachet BG-163 £ 34.00<br />
Tris Glycine running buffer 5L sachet BG-143 £ 34.00<br />
Tris Tricine running buffer (10x) solution BG-121 £ 34.00<br />
TruSep sample buffer 2 x 5mL BG-145 £ 30.00<br />
<strong>Generon</strong> Ltd., 12 Rawcliffe House, Howarth Rd, Maidenhead, Berkshire SL6 1AP<br />
Tel: (01753) 866-511 Fax: (01753) 20-88-99 E-mail: info@generon.co.uk www.generon.co.uk
50 kDa<br />
RAPID: 15 min non-toxic safe 1-step stain. No organic solvents and No phosphoric acid.<br />
SENSITIVE: 50 x more sensitive than other rapid stains. Lower limit is 5 ng protein standard.<br />
LINEAR RANGE: Very low background enabling accurate quantitation of proteins.<br />
HIGH RESOLUTION: Sharp protein bands that you would expect with traditional Coomassie staining.<br />
DURABLE: Re-usable up to 3 times! Also MS compatible.<br />
SHELF-LIFE: 1 year at Room Temperature. No precipitate forms over time so no shaking required!<br />
Order Code Description Price<br />
GEN-QC-STAIN-1L stain (1L) £88.00<br />
GEN-QC-STAIN-3L stain (3L) £225.00<br />
Tel: (01753) 866-511<br />
Fax: (01753) 20-88-99<br />
E-mail: info@generon.co.uk<br />
Simple 1-step Protocol<br />
Remove the gel from the cassette and place<br />
gel into container.<br />
Cover gel with 25 ml QC stain and leave for<br />
minimum 15 minutes or until all weak<br />
protein bands are fully developed.<br />
Store the gel in the QC stain overnight.<br />
Then, either leave in QC stain or transfer to<br />
DI water for gel storage. (N.B. A minimum 1<br />
hour full stain is recommended before<br />
storing the gel in water.)<br />
Lysed bacterial cell pellet in PBS, 1% Triton X-100 and protease inhibitors (1:500). Samples sonicated at 20% amplitude for<br />
7x20 sec on/off on ice. SDS-PAGE samples diluted 1:3 in PBS and loaded on to a 12% Nusep Tris-Gly gel supplied by<br />
<strong>Generon</strong> and stained with our QC Stain. The overexpressed 50 kDa protein is visible.<br />
<strong>Generon</strong> Ltd,<br />
12 Rawcliffe House, Howarth Road,<br />
Maidenhead, Berkshire SL6 1AP, UK.<br />
Web: www.generon.co.uk
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GeBAflex-tube for small scale and rapid dialysis<br />
Ability to dialyse any volume of sample between 10 µl and 20 ml<br />
Available as GeBaflex-tube Mini for sample volumes between 10-250 μl, GeBaflex-tube Midi for<br />
sample volumes between 50-800 μl, GeBaflex-tube Maxi for sample volumes between 100-3000 μl<br />
and GeBaflex-tube Mega for sample volumes between 3 ml and 20 ml.<br />
Simple design means that rapid dialysis is user-friendly with fewer steps and no mess. No syringes or<br />
needles are required. Safe retrieval of sample.<br />
Effective rapid dialysis<br />
Complete and reproducible dialysis within 2 hours.<br />
No air bubbles form between the sample and the dialysis membrane.<br />
NaCl (% control)<br />
The GeBAflex-tube system is a single-step<br />
dialysis tube, free of RNase, DNase, and<br />
available in 4 different volumes and<br />
different MWCOs. Dialysis of a large<br />
number of proteins can be achieved<br />
simultaneously thereby avoiding<br />
bottlenecks in applications as diverse as<br />
de-salting and buffer exchange of protein<br />
and peptide solutions to sample<br />
conditioning in drug screening studies.<br />
Dependence of [NaCl] in the GeBAflex-tube as a function of Time<br />
Time (mins)
Multiple dialysis simultaneously<br />
Multiple samples can be dialyses in parallel. Ideal for screening methodologies or drug candidates.<br />
No pre-treatment necessary<br />
Ready-to-use with no labour intensive preparation steps.<br />
High Recovery<br />
Easy sample loading and recovery and absence of hold up volume ensures high recoveries of dialysed<br />
samples. The use of premium cellulose ester dialysis membrane ensures very low non-specific protein<br />
binding. The membranes are free of heavy metals and sulphides.<br />
Specifications:<br />
Membrane type: Cellulose esters<br />
MWCO: 1 kDa (Mega only), 3.5 kDa, 8 kDa, 25 kDa (Mini and Maxi), 50 kDa<br />
(Maxi only)<br />
Working pH range: pH 2.0-9.0<br />
Maximum sample volume: 20 ml<br />
Minimum sample volume: 10 µl<br />
Storage: Store at RT (15-25 °C). Do not freeze.<br />
Shelf-life: 12 months (Shipped dry in glycerol)<br />
Dialysis time: 2 hours to overnight<br />
Ordering Information:<br />
Product Volume capacity MWCO Units Order Code Price<br />
GeBAflex-tube Mini 250 µl 8 kDa 10 D070-6-10 £33.50<br />
GeBAflex-tube Mini 250 µl 25 kDa 5 D070-25-5 £33.00<br />
GeBAflex-tube Midi 800 µl 3.5 kDa 10 D010 £52.00<br />
GeBAflex-tube Midi 800 µl 8 kDa 10 D020 £52.00<br />
GeBAflex-tube Maxi 3,000 µl 3.5 kDa 15 D035 £74.00<br />
GeBAflex-tube Maxi 3,000 µl 8 kDa 15 D045 £74.00<br />
GeBAflex-tube Maxi 3,000 µl 25 kDa 5 D060-25-5 £48.00<br />
GeBAflex-tube Maxi 3,000 µl 50 kDa 5 D060-50-5 £48.00<br />
GeBAflex-tube Mega 10 ml 1 kDa 5 D080-1/10-5 £85.00<br />
GeBAflex-tube Mega 15 ml 1 kDa 5 D080-1/15-5 £85.00<br />
GeBAflex-tube Mega 20 ml 1 kDa 5 D080-1/20-5 £85.00<br />
GeBAflex-tube Mega 10 ml 3.5 kDa 10 D080-3.5/10-10 £85.00<br />
GeBAflex-tube Mega 15 ml 3.5 kDa 10 D080-3.5/15-10 £85.00<br />
GeBAflex-tube Mega 20 ml 3.5 kDa 10 D080-3.5/20-10 £85.00<br />
GeBAflex-tube Mega 10 ml 8 kDa 10 D080-6/10-10 £85.00<br />
GeBAflex-tube Mega 15 ml 8 kDa 10 D080-6/15-10 £85.00<br />
GeBAflex-tube Mega 20 ml 8 kDa 10 D080-6/20-10 £85.00<br />
*All packs include a floating rack and handbook. The Maxi packs contain 2 caps. One cap is used for dialysing<br />
100-2000 µl sample volumes and the other cap is used for dialysing sample volumes between 2000-3000 µl.
Ultrapure Detergents<br />
Lipid Products<br />
Labelling Reagents for Crystallizations<br />
VIP Monoclonal and Polyclonal Tag Antibodies<br />
Elite Mouse and Rat Isotyping and Quantitation kits<br />
Ultra Pure Carbohydrate/Non-Carbohydrate Detergents and Reagents<br />
-Ultra pure alkyl-β-glycoside-detergents for Purifications, Reconstitutions and Crystallizations<br />
e.g. DDM, DM, OG, OTG, NG, MEGA, UDM, TDM, NM,<br />
-Non-carbohydrate Detergents and Reagents e.g. CHAPS, CHAPSO, IPTG<br />
Ultra Pure Natural and Semi-Synthetic Lipids for Micelles/Liposome/Signal<br />
Transduction Studies<br />
-Semi Synthetic Lipids e.g. DOPC, DMPC, DOPE, DPPE, DPPC etc<br />
-Natural Lipids e.g. PC, PS, PE, PI, PG, Lyso PC, Lyso PE, Lyso PG etc<br />
Ultrapure Labelling Reagents for Crystallizations<br />
L-(+)-Selenomethionine (> 98%)<br />
L-(+)-Selenocystine (> 98%)<br />
3-Iodo-L-tyrosine (>97%)<br />
VIP Monoclonal and Polyclonal Tag Antibodies<br />
Epitope-specific tag antibodies for HIS, FLAG, V5, MYC, HA, GFP recombinant proteins<br />
Elite Monoclonal Antibody Isotyping and Quantification Kits<br />
Rapid Rat and Mouse Monoclinal Isotpying kits and Mouse IgG Quantitation Kit
Detergents<br />
GENERON Detergents for Protein Purifications, Reconstitutions and Crystallizations<br />
GENERON has teamed up with Glycon, who have developed new procedures for the preparation of ultra pure<br />
alkyl-β-glycoside-detergents. All α-isomers are completely removed by chromatography. All of our detergents are<br />
crystallized to greater than 99.5% purity. The re-crystallization procedure removes all side products and<br />
lipid alcohols eg dodecanol and the final detergents are presented in a lyophilized form. There is no α−isomer<br />
present in the Glycon detergents.<br />
The anomeric purity of the detergent is critical for the isolation and crystallization of membrane proteins<br />
[P. Fromme, H. T. Witt, Improved isolation and crystallisation of Photosystem I for structural analysis; Biochem.<br />
Biophys. Acta, 1365 (1998) 175-184].<br />
NON-IONIC DETERGENTS<br />
2<br />
Size Price<br />
n-Octyl β-D-glucopyranoside (OG) > 99.5% for crystallography D97001-C<br />
n-Octyl β-D-glucoside<br />
HO<br />
HO<br />
OH<br />
O<br />
O (CH2 ) 7 CH3 OH<br />
C 14 H 28 O 6 MW 292,38 CAS 29836-26-8,<br />
CMC (H 2 O) 18-20 mM CMC (0,1M NaCl)~23,4 mM<br />
White solid crystal, [α] D -34° (c 5; H 2 O)<br />
thermal transition: 70,1°C (smectic A), 106,8°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,03<br />
Solubility: > 1 g in 10 mL H 2 O or EtOH clear, colourless<br />
solution<br />
Contaminants: n-Octanol 99.5% for crystallography D99003-C<br />
HO<br />
HO<br />
OH<br />
O<br />
HO O<br />
HO<br />
OH<br />
O (CH 2 ) 9 CH 3<br />
O<br />
OH<br />
C 22 H 42 O 11 MW 482,57 CAS 82494-09-5, CMC (H 2 O) 1,8 mM<br />
White solid crystal, [α] D +53,0° (c 4; H 2 O)<br />
thermal transition: 89,2°C (smectic A), 208°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,04<br />
Solubility: 1 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Contaminants: n-Decanol 99.5% for crystallography D97002-C<br />
Lauryl maltoside<br />
HO<br />
HO<br />
OH<br />
O<br />
HO O<br />
HO<br />
OH<br />
O (CH 2 ) 11 CH 3<br />
O<br />
OH<br />
C 24 H 46 O 11 MW 510,63 CAS 69227-93-6,<br />
CMC (H 2 O) 0,17 mM<br />
White solid crystal, [α] D +48°(c 1; H 2 O)<br />
thermal transition: 103°C (smectic A), 246°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,04, Aggregation No.: 98<br />
Solubility: 1 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Contaminants: n-Dodecanol
GENERON Detergents for Protein Purifications, Reconstitutions and Crystallizations<br />
Alkyl 1-thio-β-glycosides<br />
Size Price<br />
n-Octyl 1-thio-β-D-glucopyranoside (OTG) D20014<br />
n-Octyl-β-D-thioglucopyranoside (n-Octyl-β-D-thioglucoside)<br />
HO<br />
HO<br />
OH<br />
O<br />
S (CH2 ) 7 CH3 OH<br />
C 14 H 28 O 5 S MW 308,44 CAS 85618-21-9 CMC (H 2 O) ~9 mM<br />
White solid crystal, m.p. 128°C, [α] D -53° (c 1; MeOH)<br />
Absorbance (0,5%, H 2 O, 260 nm): < 0,25<br />
Solubility: > 8 g in 100 mL H 2 O clear, colourless solution<br />
Contaminants: n-Octanthiol 99.5% for crystallography D99012-C<br />
HO<br />
HO<br />
OH<br />
O<br />
HO OH<br />
O<br />
O (CH2 ) 10CH3 HO O<br />
HO<br />
C 23 H 44 O 11 MW 496,59 CAS 170552-39-3, CMC (H 2 O) 0,59<br />
mM<br />
White solid crystal, [�] D +50° (c 1; H 2 O)<br />
thermal transition: 96°C (smectic A), 236°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,04<br />
Solubility: 1 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Contaminants: n-Undecanol 99.5% for crystallography D20016-C<br />
HO<br />
HO<br />
OH<br />
O<br />
HO OH<br />
O<br />
O (CH2 ) 12CH3 HO O<br />
HO<br />
C 25 H 48 O 11 MW 524,64 CMC (H 2 O) 0,033 mM<br />
White solid crystal, [�] D +45,5° (c 1; H 2 O)<br />
thermal transition: 110°C (smectic A), 255°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,04, Aggregation No.: 105<br />
Solubility: 1 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Contaminants: n-Tridecanol 99.5% for crystallography D99011-C<br />
HO<br />
HO<br />
OH<br />
O<br />
O (CH ) CH<br />
2 8 3<br />
OH<br />
C 15 H 30 O 6 MW 306,40 CAS 69984-73-2, CMC (H 2 O) 18-20<br />
mM<br />
White solid crystal, [�] D -33° (c 5; H 2 O)<br />
thermal transition: 72,3°C (smectic A), 118,1°C (isotropic)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,03<br />
Solubility: > 1 g in 10 mL H 2 O or EtOH clear, colourless<br />
solution<br />
Contaminants: n-Nonanol 99.5% for crystallography D20015-C<br />
HO<br />
HO<br />
C 21 H 40 O 11 MW 468,41 CMC (H 2 O) 6 mMol<br />
OH<br />
O<br />
White solid crystal, [�] D +55° (c 1; H2O) thermal transition: 81°C (smectic A), 197°C (isotropic)<br />
HO<br />
O<br />
OH<br />
O (CH2 ) 8CH Absorbance (1%, H<br />
3<br />
2O, 280 nm): < 0,04, Aggregation No.: 55<br />
HO<br />
HO<br />
O Solubility: 1 g/ 10 mL H2O, 21°C clear, colourless solution<br />
Contaminants: n-Nonanol
GENERON Detergents for Protein Purifications, Reconstitutions and Crystallizations<br />
N-(n-Alkanoyl)-N-methylglucamines – MEGA<br />
MEGA-8 (N-Octanoyl-N-methylglucamin) D98004<br />
HO<br />
HO<br />
OH<br />
OH<br />
N<br />
OH<br />
O<br />
C 15 H 31 NO 6 MW 321,42 CAS [5316-98-9]<br />
White solid CMC (H 2 O) ~9 mM 1,2 m.p.<br />
88°C, [α] D -17,7°(c 2; H 2 O)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,01<br />
Solubility: >2 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Purity (HPLC): >99.5%<br />
1,2<br />
1 g £30<br />
10 g £155<br />
25 g £308<br />
100 g £980<br />
BULK available<br />
MEGA-9 (N-Nonanoyl-N-methylglucamin) D99005<br />
HO<br />
HO<br />
OH<br />
OH<br />
N<br />
OH<br />
O<br />
C 16 H 33 NO 6 MW 335,44 CAS [85261-19-4]<br />
White solid crystal CMC (H 2 O)~ 25 mM<br />
m.p. 89°C, [α] D -17,0°(c 2; H 2 O)<br />
Absorbance (1%, H 2 O, 260 nm): < 0,01<br />
Solubility: >2 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Purity (HPLC): >99.5%<br />
1 g £30<br />
10 g £155<br />
25 g £308<br />
100 g £980<br />
BULK available<br />
MEGA-10 (N-Decanoyl-N-methylglucamin) D99008<br />
HO<br />
HO<br />
CHAPS<br />
OH<br />
OH<br />
N<br />
OH<br />
O<br />
C17H35NO6 MW 349,47 CAS [85261-20-7]<br />
White solid crystal CMC (H2O) ~6-7 mMol/L<br />
m.p. 91°-93°C, [α] D -16,0°(c 1; H2O) 1 g<br />
10 g<br />
25 g<br />
£30<br />
£155<br />
£308<br />
Absorbance (1%, H2O, 260 nm): < 0,01<br />
100 g £980<br />
Solubility: >1 g/ 10 mL H 2 O, 21°C clear, colourless solution<br />
Purity (HPLC): >99.5%<br />
IONIC DETERGENTS<br />
BULK available<br />
Size Price<br />
3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate D99009<br />
Me<br />
HO Me H<br />
Me H<br />
H H<br />
HO OH<br />
H<br />
O<br />
R = N N + SO3 H Me M e<br />
R<br />
-<br />
C 32 H 58 N 2 O 7 S MW 614,89 CAS 75621-03-3<br />
White solid crystal CMC (H2O)~8mM 1 Aggregation No.: 10<br />
Absorbance (1 cm / 5%, H 2 O) 290 nm < 0,5; 400 nm 0,02<br />
Pb < 0,005% Na < 0,005% N 4,5% H 2 O 2%<br />
Purity (HPLC): > 99%<br />
CHAPS is a zwitterionic detergent with non denaturing<br />
properties. It is especially useful for solubilization of membrane<br />
proteins. It is used as a 1 -13 mM solution.<br />
1 g £42<br />
10 g £78<br />
25 g £170<br />
100 g £582
GENERON Detergents for Protein Purifications, Reconstitutions and Crystallizations<br />
DETERGENT ANALYSES<br />
All of our detergents are crystallised to be greater than 99.5% pure as measured by TLC, HPLC,<br />
double melting point and by the following analytical procedures.<br />
Measurement of purity (HPLC, TLC)<br />
Each lot is first analysed by TLC and HPLC. All detergents are greater than 99.5% pure. TLC was<br />
performed on silica gel (Merck 60 F (254), dichloromethane/methanol 7:1) and detection was effected<br />
by spraying the TLC plate with a solution of ethanol-sulfuric acid (9:1) followed by heating. HPLC was<br />
performed on a standard C18 column with an eluant of methanol/water. The ration of methanol to<br />
water may vary from 65/35 to 85/15 depending on the hydrophobicity of the detergent. A 50% solution<br />
of detergent in MeOH is injected into the HPLC system. [P. Fromme, H. T. Witt, Improved isolation and<br />
crystallisation of Photosystem I for structural analysis; BBA, 1365 (1998) 175-184].<br />
Alcohol and α-isomer contamination<br />
β-n-Alkylglycoside detergents are prepared by glycosylation of the hydrophobic alcohol with activated<br />
glucose or maltose acetates. Nearly all products are purified chromatographically and by<br />
crystallisation. Alcohols and α-isomers are completely removed during the manufacturing process.<br />
Trace amounts of this alcohol in the detergent lot can cause cloudiness in the detergent solution. The<br />
β-anomeric purity of the detergent is advantageous for the crystallization of membrane proteins.<br />
Measurement of purity (Double melting point)<br />
The double melting properties of β-n-alkylglycosides have long been known (E. Fischer, B. Helferich;<br />
Liebigs Ann. Chem. 383, 68 (1911). Alkylglycosides have liquid crystal properties and they can form<br />
mesophases in the melt (a state of order between crystals and liquids). In the smectic A mesophase<br />
the amphiphilic rod-like molecules form layered structures - a microscopic separation by a sequence<br />
of hydrophilic and lipophilic layers. In the isotropic phase, the molecules are packed as a liquid in<br />
a random way. An optical polarised microscope equipped with a hotstage and a central processor<br />
is used to identify transition temperatures from the smectic A to the isotropic phase. Measurement of<br />
transition temperatures (double melting temperatures) gives a very sensitive test for the contamination<br />
of the crystalline detergent with hydrophobic alcohols, α-glycosides, solvents and others organic/<br />
inorganic impurities.<br />
Absorbance<br />
Alkyl-glucosides and -maltosides have low absorbance throughout the UV region. MEGA detergents,<br />
containing amido group, have high absorbance at 230 nm. The absorbance of the 1% w/v detergent<br />
solution in water is measured in the UV region.<br />
Fluorescence<br />
A very low level of aromatic impurity can still result in a large fluorescence background. The<br />
fluorescence of the detergent solution is compared to a standard 0.1% BSA solution. The excitation<br />
wavelength is 280 nm and the emission is measured at 345 nm.<br />
Conductance<br />
Measurement of conductance gives a very sensitive test for the presence of ionic impurities. A solution<br />
of non-ionic detergent should have conductance nearly the same as de-ionised water.<br />
Measurement of pH<br />
The pH of the detergent solution at appropriate concentration is measured. The pH value of the<br />
solution should be neutral.<br />
Solubility in water<br />
Some of the impurities (e.g. hydrophobic alcohol) in detergent preparations are not soluble in water.<br />
Therefore cloudiness of a detergent solution at a concentration where it is known to be soluble<br />
indicates the presence of an insoluble impurity.<br />
Address: 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire SL6 1AP.<br />
Tel: 01753-866-511 Fax: 01753-20-88-99 E-mail: info@generon.co.uk Web: www.generon.co.uk
Figure show purity ofβ-DM by HPLC (Glycon)<br />
Solvent: 72% Methanol + 28% Water<br />
Results: The peak at 24.5 min is the β-isomer only. There is no α-isomer peak.<br />
Address: 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire SL6 1AP.<br />
Tel: 01753-866-511 Fax: 01753-20-88-99 E-mail: info@generon.co.uk Web: www.generon.co.uk
Specifications:<br />
Ultrapure IPTG: Isopropyl 1-thio-β-D galactopyranoside<br />
Isopropyl 1-thio-β-D galactopyranoside<br />
Description Specification<br />
IPTG induces the transcription of the gene<br />
coding for β-galactosidase, an enzyme that<br />
promotes lactose utilization, by binding and<br />
inhibiting the LacI repressor. IPTG is widely<br />
used as it cannot be metabolized by E. coli<br />
so its concentration remains constant<br />
through the in vivo study. We offer ultra-<br />
pure IPTG from Glycon.<br />
Appearance: White crystalline powder<br />
Formula: C9H1805S<br />
Molecular weight: 238.30 g/mol<br />
CAS: [367-93-1]<br />
IR: Standard<br />
Specific rotation (20°C, c=1, H2O): -31 to -33°<br />
Purity (HPLC, A220) >99.0% (typically 99.5%)<br />
pH (1% solution) 5.5-6.5<br />
Water (K.F.) max. 0.5%<br />
Dioxan: Dioxan is not used for preparing IPTG.<br />
Solution (3% in H2O, 21°C) Clear, colourless<br />
Solution (20% in EtOH, 21°C) Clear, colourless<br />
TLC (CH2Cl2: MeOH 3:1) One spot Rf 0.5 ± 0.1<br />
Order Code Description Price<br />
GEN-S-02122 Ultrapure IPTG (> 99%; 25 g) £82.50
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SeMet SeCys<br />
Specifications:<br />
L-(+)-Selenomethionine<br />
L-Selenomethioine and 3-Iodo-L-tyrosine<br />
Order Code Description Price<br />
3211-76-5 L-(+)-Selenomethionine > 98+% (2.5 g) £175.00<br />
50-50-250 L-Selenocystine > 98+% (250 mg) £38.00<br />
50-50-1000 L-Selenocystine > 98+% (1 g) £98.00<br />
70-78-0 3-Iodo-L-Tyrosine > 97+% (25 g) £312.00<br />
Tel: (01753) 866-511<br />
Fax: (01753) 20-88-99<br />
E-mail: info@generon.co.uk<br />
L-Selenomethionine (SeMet) and L-Selenocystine (SeCys) are<br />
used to label proteins during cell culture and expression.<br />
Incorporation of these amino acid analogues into target<br />
proteins aids structure determination by X-ray<br />
crystallographic methods, using the well-established<br />
anomalous scattering approach (1, 2).<br />
1: Hendrickson & Ogata (1997) Methods in Enzymology Macromolecular Crystallography Part A.<br />
276, 1997, Pages 494-523. Phase determination from multiwavelength anomalous diffraction<br />
measurements.<br />
2: Salgado et al (2011) Acta Cryst. D67, 8-13. Extending the usability of the phasing power of<br />
diselenide bonds: SeCys SAD phasing of CsgC using a non-auxotrophic strain.<br />
Appearance: White to off-white crystalline<br />
powder<br />
IR: Conform with standard<br />
Specific rotation: +17 to +19.5°<br />
(20°C, 589 nm, c=1, 1 N HCl)<br />
MR (decomp.): 262-267°C<br />
Selenium content: 39-41 %<br />
Water: < 0.5 %<br />
Assay (non-aq.titr.) 98.0-100.5%<br />
Assay (HPLC) >98%<br />
Arsenic
VIP Monoclonal and Polyclonal Tag Antibodies<br />
GENERON offer a range of high quality VIP Tag Antibodies, specific for a wide range of<br />
recombinant proteins. These monoclonal and polyclonal antibodies recognise specific epitope<br />
tags used in the study of protein expression in various systems. Ideal for detecting fusion<br />
protein expression in cells.<br />
Product Code Product<br />
Description<br />
VIP-HIS-Mab HIS Tag Ab<br />
(clone 6AT18)<br />
VIP-FLAG-Pab<br />
VIP-V5-Pab<br />
VIP-HA-Mab<br />
VIP-GFP-Mab<br />
VIP-MYC-Mab<br />
FLAG Tag Ab<br />
(clone RB0493)<br />
V5 Tag Ab<br />
(clone RB0413)<br />
HA Tag Ab<br />
(clone 12CA5)<br />
GFP Tag Ab<br />
(clone 168AT1211)<br />
Myc Tag Ab<br />
(clone 9E10)<br />
Quantity Type £ Price<br />
0.1mg Mouse Monoclonal Ab<br />
(IgG1, 0.25mg/ml)<br />
0.1mg Rabbit Polyclonal Ab<br />
(0.25mg/ml)<br />
0.1mg Rabbit Polyclonal Ab<br />
(0.25mg/ml)<br />
0.1mg Mouse Monoclonal Ab<br />
(IgG2b, 0.25mg/ml)<br />
0.1mg Mouse Monoclonal Ab<br />
(IgG1, 0.25mg/ml)<br />
0.1mg Mouse Monoclonal Ab<br />
(IgG1, 0.25mg/ml)<br />
£119.00<br />
£119.00<br />
£119.00<br />
£119.00<br />
£119.00<br />
£119.00<br />
<strong>Generon</strong> Ltd, 12 Rawcliffe House, Howarth Road,<br />
Maidenhead, Berkshire, SL6 1AP, UK.<br />
Tel: (01753) 866511 Fax: (01753) 208899<br />
Email: info@generon.co.uk <strong>Catalogue</strong>: www.generon.co.uk
Elite Monoclonal Antibody Isotyping and Quantification Kits<br />
GENERON offers rapid MOUSE and RAT MONOCLONAL ANTIBODY ISOTYPING TEST KITs and a MOUSE<br />
MONOCLONAL SEMI-QUANTITATION KIT.<br />
Mouse and Rat Dipstick Isotyping Kits Mouse Monoclonal IgG Semi-Quant Kit<br />
� Kits for mouse and rat monoclonals. Dipstick mouse semi-quantitative kit.<br />
� For hybridoma supernatants and serum For hybridoma supernatants and serum.<br />
� Results in 5-10 minutes. No sample preparation required.<br />
� Sensitivity threshold of 1.0 µg/ml. Results in 7-10 minutes.<br />
� Detects both κ and λ light-chains. Sensitivity from 15 ng/ml to 1,000 ng/ml.<br />
� Detects IgG1, 2a, 2b, 3, IgM and IgA. Detects total IgG (IgG1, 2a, 2b and 3).<br />
� Positive blue flow control band. Designed to give you an idea of the IgG range.<br />
Product Code Product<br />
Description<br />
GEN-20110 Rapid Type- M<br />
Mouse Isotyping<br />
Kit<br />
Rapid Mouse IgG<br />
GEN-20210 Quantitation<br />
Test Kit<br />
GEN-20120 Rapid Type-R<br />
Rat Isotyping Kit<br />
Quantity Target Species £ Price<br />
10 Tests Mouse £169.00<br />
20 Tests Mouse £195.00<br />
10 Tests Rat £195.00<br />
<strong>Generon</strong> Ltd, 12 Rawcliffe House, Howarth Road,<br />
Maidenhead, Berkshire, SL6 1AP, UK.<br />
Tel: (01753) 866511 Fax: (01753) 208899<br />
Email: info@generon.co.uk <strong>Catalogue</strong>: www.generon.co.uk
Elite Protein Expression Service<br />
Custom Peptide Synthesis Service<br />
Custom Monoclonal Antibody Service<br />
Comprehensive Unlabelled and 15 N-labelled Protein Expression/Purification/<br />
Characterization Service<br />
� Full Customer and Trouble-Shooting Support<br />
� E.coli, Insect and Mammalian Expression Platforms available<br />
� Accession number /sequence ID, PCR amplification, Cloning, Colony Screening and<br />
Sequencing.<br />
� Protein Mini-expression/Optimization, Bulk Up, Purification and Tag Removal.<br />
� Final Stage Protein Clean Up/Biophysical Analysis e.g. MS, CD-Spectroscopy, Light<br />
Scattering<br />
� 15 N labelled protein expression and purification service for 15 N-HSQC protein NMR.<br />
Custom Peptide Synthesis Service<br />
� Premium & Rapid custom peptide synthesis service.<br />
� Specialists in long and modified peptides.<br />
Custom Monoclonal Antibody Service: ‘Using Protein-free Media’<br />
� Unique monoclonal antibody production and development in UK.<br />
� Full peptide design, synthesis and conjugation facitlity<br />
� Modular Pricing. Full customer and troubleshooting support.
NMR update: 15 N-labelled<br />
protein expression for<br />
HSQC now available!<br />
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Custom Monoclonal Antibody Service:<br />
‘Using Protein-free Media’<br />
GENERON UNIQUE SERVICE OFFERING: We offer a distinct ‘protein-free’<br />
monoclonal antibody production and development service based in the UK.<br />
Benefit from full client and project support with communications and updates on<br />
a regular basis. Full peptide design, synthesis, and conjugation facility. Modular<br />
pricing structure to ensure we meet your exact requirements. Clients are<br />
welcome to visit facilities and meet the scientific team.<br />
ANTIBODY EXPERTISE: With our experience in providing antibody production and<br />
purification services to clients, we deliver a complete service from immunization<br />
all the way through to high quality production of monoclonal antibody in protein<br />
free media.<br />
BESPOKE QUALITY SERVICE: We work hand in hand with you to generate the best<br />
quality antibody. This includes full client support and troubleshooting.
1. Antigen Design and Immunisation<br />
Phase<br />
� Discuss project strategy with our technical<br />
team.<br />
� Scope requirements for (i) the initial<br />
immunisation phase, (ii) design and preparation<br />
of antigens. Peptide / conjugation design advice<br />
available.<br />
� Quantify serum titer by ELISA, Western Blot.<br />
2. Cell Fusion & Screening of<br />
Colonies Phase<br />
� Hybridoma fusion of mobile lymphocytes into<br />
10x96 well plates.<br />
� ELISA screen to select strongest positive<br />
candidate 'binders'.<br />
� 24-well plate expansion to harvest tissue<br />
culture supernatant.<br />
3. Limiting Dilution Cloning and<br />
Cryopreservation Phase<br />
� Limiting dilution cloning performed with<br />
strongest parental colonies to create<br />
homogeneous populations.<br />
4. Large Scale Antibody Production<br />
Phase<br />
� Production performed in vitro using a variety of<br />
bioreactor systems with option for protein-free<br />
adaptation.<br />
� Purification using immuno-affinity<br />
chromatography.<br />
1. Timescale: Complete in 6-10 weeks<br />
� Generate rat or mouse antisera. Assay by ELISA.<br />
All work carried out in UK lab.<br />
� Serum samples can be sent to the client for<br />
rapid verification.<br />
� Proceed to fusion following positive response<br />
from animal. Cells kept at the facility.<br />
2. Timescale: Complete in 3-6 weeks<br />
� Generate myeloma fusion prep.<br />
� Initial assay by ELISA of up to 960 colonies.<br />
� Typically identify 20-30 'candidate' binders.<br />
� Antibody screening methods including ELISA,<br />
Western Blot or FACS. Select isotype-specific<br />
antibodies.<br />
3. Timescale: Complete in 3-5 weeks<br />
� Subclones with high specificity expanded and<br />
cryopreserved.<br />
� Shipment of hybridoma cells and supernatant<br />
fraction from each subcloned cell line.<br />
� Production of 5mg of pure mAb from the best<br />
subclone.<br />
4. Timescale: Complete in 3-6 weeks<br />
� Milligramme to gramme quantities produced.<br />
� Standard 1 litre batch culture and purification<br />
typically yields 25-40mg<br />
� Please note that all scale up is done in proteinfree<br />
media to deliver mouse IgG only.<br />
� NB. We also offer rat monoclonals.<br />
<strong>Generon</strong> Ltd, 12 Rawcliffe House, Howarth Road,<br />
Maidenhead, Berkshire, SL6 1AP, UK.<br />
Tel: (01753) 866511 Fax: (01753) 208899<br />
Email: info@generon.co.uk <strong>Catalogue</strong>: www.generon.co.uk
<strong>Generon</strong> offers custom polyclonal<br />
antibody services to run alongside its<br />
monoclonal antibody production.<br />
The polyclonal service is available in<br />
rabbit, chicken and goat host species.<br />
How to select the correct Host Species?<br />
Quality Custom Polyclonal<br />
Antibody Production<br />
Rabbit Goat Chicken<br />
Most common species Ideal for large volumes Non-mammalian<br />
Less background in crude Long life span Non-invasive (egg yolks)<br />
serum than goat<br />
Good for peptide immunogens Titres tend to be lower in goats IgY purification available.<br />
than rabbits. Not ideal for<br />
modified peptides.<br />
Not ideal for highly conserved Ideal for highly conserved antigens Often 5 to 10 times more antibody<br />
mammalian antigens than rabbits<br />
Key Service:<br />
Host Species Minimum Serum Supplied Antibody supplied Purification<br />
Amount/Concentration per Collection (days) Available<br />
of Immunogen required<br />
Rabbit 1.5 mg @ 1 mg/ml ~25 ml 56 Yes<br />
Goat 2.5 mg @ 1 mg/ml ~250 ml 56 Yes<br />
Chicken 1.5 mg @ 1 mg/ml ~1 ml 56 Yes<br />
Features<br />
For Rabbits and Goats, the 56 day protocol includes 1 preimmune<br />
bleed, 4 immunizations and 3 production bleeds for<br />
rabbits and goats with a first production bleed in 35 days.<br />
For Chickens, the 56 day protocol includes 1 pre-immune<br />
bleed, 4 immunizations, 3 test bleeds and egg collection<br />
(approximately 14 eggs). IgY purification from egg yolks is also<br />
available.<br />
Why choose <strong>Generon</strong>?<br />
Health of animals is paramount.<br />
The animals are bred and raised in open barns.<br />
A healthy animal is more likely to produce better antibodies.<br />
Serum purification with Protein A, Protein G and immunoaffinity<br />
purifications using peptides.<br />
Conjugation of antibodies with a wide variety of labels.<br />
ELISA report on antibody titres. Western Blot antibody<br />
validation using endogenous protein lysates.<br />
Peptide design and synthesis is also available. Peptides are<br />
conjugated to a carrier protein such as KLH or BSA.<br />
All the farms are registered in US and are inspected regularly by<br />
the USDA.<br />
Guarantee antibody titre of at least 15,000:1 to the<br />
immunogen as measured by ELISA.<br />
<strong>Generon</strong> Ltd, 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire, SL6 1AP, UK.<br />
Tel: (01753) 866511 Fax: (01753) 208899<br />
Email: info@generon.co.uk Web: www.generon.co.uk