2nd ASEAN Sago Symposium 2012, UNIMAS, Kota Samarahan ...
2nd ASEAN Sago Symposium 2012, UNIMAS, Kota Samarahan ...
2nd ASEAN Sago Symposium 2012, UNIMAS, Kota Samarahan ...
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2 nd <strong>ASEAN</strong> <strong>Sago</strong> <strong>Symposium</strong> <strong>2012</strong>, <strong>UNIMAS</strong>, <strong>Kota</strong> <strong>Samarahan</strong><br />
Advances in <strong>Sago</strong> Research and Development<br />
Poster 8<br />
<strong>Sago</strong> and Papua Local Wisdom.<br />
Nicolaas Filo Maniagasi*<br />
Papua Biodiversity Foundation, Komp. BTN Skyland Indah,No. 76, <strong>Kota</strong>-Raya,<br />
Jayapura, Papua INDONESIA.<br />
Email address of corresponding author: nnmaniagasi@yahoo.com<br />
Papua Indonesia has sago nature 1.200.000 ha or 53% of totality 2.250.000 ha sago nature of<br />
the World. This sago nature potential should be utilized either. There is the experience of native<br />
Papua can we notice about sago. <strong>Sago</strong> nature should be protected for the world.<br />
Poster 9<br />
Physiological Performance of Nursery Stage Spine and Spineless <strong>Sago</strong>.<br />
Mohd Roslan Md Noor*, Maizan Ismail, Wahid Omar & Ahmad Kushairi Din<br />
Biological Research Division, Malaysian Palm Oil Board,<br />
6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, MALAYSIA<br />
*Email address of corresponding author: mroslan@mpob.gov.my<br />
Physiological performance of two types of sago planting materials namely the spine and<br />
spineless sago were evaluated based on gas exchange measurements and water use efficiency<br />
(WUE). Both planting materials were raised on concrete beds filled with 6cm depth of water<br />
under rain shelter. Gas exchange measurements were done using the LICOR 6400 (Nebraska,<br />
USA) on leaflets from frond No 2. Results showed that the CO2 assimilation rate of spineless<br />
sago was 11% higher than the spine, 18% higher in WUE and 12.5% higher in stomatal<br />
conductance.<br />
Poster 10<br />
Construction of Metroxylon sagu Cysteine Protease cDNA into expression vector pET41a+<br />
Nurul ‘Izzati binti Chik* & Hairul Azman Roslan<br />
Department of Molecular Biology, Faculty of Resource Science and Technology,<br />
Universiti Malaysia Sarawak, 94300 <strong>Kota</strong> <strong>Samarahan</strong><br />
Sarawak MALAYSIA.<br />
*Email address of corresponding author: izzati89_chik@yahoo.com<br />
Protease is a group of enzymes that degrades polypeptides. The enzyme cysteine protease in<br />
particular has many roles in plant cells physiology and development such as in embryogenesis,<br />
tracheary element differentiation, germination of seeds, leaf and flower senescence, and in<br />
response to biotic and abiotic stresses. Cysteine protease was found to be present in sago palm<br />
previously via transcriptome analysis of young sago palm leaf. In this project, the main aim is to<br />
construct Metroxylon sagu Cysteine Protease cDNA into expression vector pET41a+. For this<br />
work a complementary DNA (cDNA) library was used to identify and clone cysteine protease<br />
cDNA into pET41a+. This is done by employing the Polymerase Chain Reaction (PCR) method,<br />
plasmid transformation method and restriction enzyme method. Construction of cysteine<br />
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