Enteroclon Anti-Salmonella A-67, omnivalent - SIFIN

Enteroclon Anti-Salmonella A-67, omnivalent
The optimal screening reagent for
suspicious Salmonella isolates from food and
other samples — quick, simple, economical.
Microbiology
Bacteriological
Test Reagents
100 % sensitivity – covers
all Salmonella serotypes
Excellent reproducibility
Strong agglutination
Result can be read off at
the latest after 20 seconds
Only the simplest laboratory
equipment needed
Only involves a few steps

Enteroclon Anti-Salmonella A-67, omnivalent
Salmonellosis is one of the most frequent
infectious diseases caused by
food and even in industrial countries
its occurrence is tending upwards.
Proceeding in accordance with § 64
German Food and Feed Code, salmonellae
are fi rst isolated by preenrichment,
enrichment and subsequent
cultivation on selective media, and
then subjected to biochemical and serological
confi rmation.
Principle of the serotyping test
Enteroclon Anti-Salmonella A-67,
omnivalent is a mixture of 48 monoclonal
antibodies. It is manufactured
from cell culture supernatants of hybridoma
cell lines which secrete antibodies
against the corresponding
group-specifi c Salmonella O antigens
or the Vi antigen.
Enteroclon is the term used by our
company to designate products that
are prepared exclusively from monoclonal
antibodies. If the strain that is
isolated possesses a Salmonella antigen
which is covered by Enteroclon
Anti-Salmonella A-67, omnivalent,
this antigen becomes bound when
mixed with the specifi c antibody. The
antigen-antibody reaction causes the
strain to agglutinate in a clearly visible
fashion.
Materials
For the serological confi rmation test
only simple laboratory equipment is
required:
– glass slides
– stirring rods
– physiological saline
Institut für Immunpräparate
und Nährmedien GmbH Berlin
Berliner Allee 317 - 321
13088 Berlin
Germany
Fon + 49 - 30 - 92 70 30 0
Fax + 49 - 30 - 92 70 30 30
Mail info@sifi n.de
Web www.sifi n.de
How to perform the test
Place one drop of
the test reagent onto
a slide
➡ ➡ ➡
Add a small amount of
bacterial mass
from a suspicious colony
and mix it in so that a
homogeneneous, slightly milky
suspension results
Holding the slide by hand,
tilt it back and
forth 1-20 times
in front of a light source
and then inspect it with
the naked eye
The result is available
in almost no time at all
Negative: The suspension remains
milky-opaque even after it has been
tilted back and forth 20 times.
A negative result shows with certainty
that the isolate is not Salmonella (diagnosis
by exclusion).
Positive: The suspension agglutinates
after it has been tilted back and
forth between once and 20 times, or
in the case of a strongly positive reaction,
even while it is being mixed.
Specifi city: In exceptional cases, antigen
identities or related antigens may
lead to cross-reactions with other genera
of the Enterobacteriaceae family
(e.g. with strains of Citrobacter spp.,
Morganella spp. or Proteus spp.). For
this reason, it is necessary to ensure
that the sample belongs to the Salmonella
genus biochemically (see the
scheme below, right).
The classical agglutination test
using
Enteroclon Anti-Salmonella A-67,
omnivalent
is the rapid test
‚par excellence‘
Products
Further serological differentiation is
performed using the group-specifi c
and monospecifi c Enteroclons or test
sera in accordance with the Kauffmann-White
Scheme.
Product Number
Enteroclon Anti-Salmonella
(A-67), omnivalent
Enteroclon Anti-Salmonella
I (A-E)
Enteroclon Anti-Salmonella
II (F-67)
Enteroclon Anti-Salmonella
Poly-H Phase 1 & 2
Test reagents for serological
differentiation
Buffered
Peptone Water
Magnesium Chloride
Malachite Green Broth
acc. to Rappaport-
Vassiliadis (RV-Medium)
Selenit Cystine Bouillon
Brilliant Green Phenol Red
Agar acc. to
Edel and Kampelmacher
(BPLS-Agar, mod.)
MSRV Medium Base (Modifi
ed Semi-Solid Rappoport
Vassiliadis Medium Base)
Desoxycholate Citrate Agar
(DCLS-Agar), Modifi ed
XLD-Agar acc. to ISO 6579
(Xylose Lysine
Deoxycholate Agar)
SS-Agar, modifi ed (Salmonella-Shigella
Agar, mod.)
Swarm Agar
Literature
TR 1101
TR 1105
TR 1111
TR 1115
TR 1121
TR 1125
TR 1141
TR 1145
acc. to
catalogue
TN 1137
TN 1226
TN 1157
TN 1183
TN 1110
TN 1272
TN 1121
TN 1122
TN 1196
TN 1221
1 ml
5 ml
1 ml
5 ml
1 ml
5 ml
1 ml
5 ml
500 g
5 kg
500 g
500 g
500 g
500 g
500 g
5 kg
500 g
500 g
TN 1702 20 x 10 ml
Biochemical Confi rmation
Genus LDC KCN PYR NPA
Salmonella + - - -
Citrobacter - + + -
Proteus - + - +
Morganella - + - +
Lysine decarboxylase
Potassiumcyanide test
Pyrase test
Phenylalanine desaminase
Size
Mohr, J und G Pollex: Agglutinating monoclonal
antibodies in diagnosis of salmonelloses. Biotest
Bulletin 6: 75-83 (1998)
Date of revision: October 2007