PNP spectrochemistry Prelab assignment, Spring 2010 Page 1 PNP ...
PNP spectrochemistry Prelab assignment, Spring 2010 Page 1 PNP ...
PNP spectrochemistry Prelab assignment, Spring 2010 Page 1 PNP ...
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Sample calculation for problem 1<br />
For pH 6.5:<br />
<br />
<br />
6.5 6.82 <br />
<br />
0.32 <br />
<br />
0.47863 <br />
<br />
We have 2 variables, and 2 equations:<br />
Equation 1: Equation 2:<br />
A‐+HA = 5 mL A‐/HA = ratio (in this case 0.47863)<br />
Start:<br />
A‐/HA=0.47863<br />
A‐=0.47863HA<br />
Plug this into equation 1<br />
0.47863HA + HA = 5<br />
1.47863HA =5<br />
HA = 3.4<br />
Plug this into Equation 1:<br />
A‐+3.4 = 5.0<br />
A‐ = 1.6<br />
For problem 2, use M1V1 = M2V2 where M1=100 uM, M2=value in column 5, and V2=2000 uL. Solve for<br />
V1. Volume of pH 10 buffer is simply 2000 uL – V1.<br />
<strong>PNP</strong> <strong>spectrochemistry</strong> <strong>Prelab</strong> <strong>assignment</strong>, <strong>Spring</strong> <strong>2010</strong> <strong>Page</strong> 2
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