Effects of polypeptide-k supplemented soft bun on blood glucose ...

lnrernali<strong>of</strong>al Joumal ol Nut tion and Metabolism Vol. 3(1 ), pp. 7-1 0, January 201 1
Avaibble <strong>of</strong> line hllp//www.academicjounals.org/ijnam
lSgN 21 41-2340 O201 I Academic Journals
Full Length Research Paper
<strong>Effects</strong> <strong>of</strong> <strong>polypeptide</strong>-k <strong>supplemented</strong> s<strong>of</strong>t <strong>bun</strong> on
blood glucose level in healthy adults
Lee Cheng Lokl, M. D. Yong Yean Slrnt, M. D. zurainlAhmad2, Azhar Yaacob3 and
Muhammad Nazrul Haklm"'
-
'WettAgain Clinic Holistic Healthcaro, 46150, P€taling Jaya, [,4alaysia.
'Dopartment <strong>of</strong> Biomedical Scioncas, Facully ol l\r€dicin€ and Health Sciences, Universiti Pulra l,,lalaysia,
43400 UPM Sordang, Malaysia.
3sports Academy, Unlversiti Putra lvlaiaysi , €4Ob UPNI Serdang, [,4alaysia.
Ac@pred 7 January, 201 l
The alm ol thls study llr to ovaluate lho <strong>of</strong>focts <strong>of</strong> Polypoptlde-k (Ppk) suppl€montod s<strong>of</strong>l roll on lhs
blood glucose level<strong>of</strong> 18 haalthy Indlvldu.l..s comprred to control sott roll alone. Healthy Indlvlduals
were fasted ovornlght lnd blood was taken at basellns (0 mlh) befors cohsumptlon <strong>of</strong> conlrol s<strong>of</strong>t roll,
and at 0, 30, 90, 150 and 210 mln. Glucoso lovsl was thsn dstehlhgd. Thl6 proo€duro waa ropoatod wlth
Ppk strpplemanled 6<strong>of</strong>t rcll on th€ same Indlvldual3 the next d.y. Ppk supplomonlod roll .oll showod
slghlllcant docrotnghl (p

lrf J. Nutr. Metab.
9 ,.
€
Ej
=0
g
U
; -1.s
nme{mlnutesl
-conkols<strong>of</strong>tRoll - Polypeptlde s<strong>of</strong>t Roll
Flguro 1, G€ph olblood qllcoso levellrcm basollne vorsus llrn€
medrinalhefb and/or vegelable for treaiment ol diabolos
In gouth€ast
UATERIALS AND METHODS
Asian colnlries (Sonanayake et al,, 2004). ooypeprlde.k
hs hypoglycemic acuvlty has b€€n reported in pulps,
{oxract6d lrcm M. chaqada war prcvided by
Magna Blo-Laboratori€s, Varaysla). Son rols lomLlal€d
seeds ari leavos ln v/vo (Xlang ol a1.,2007). Khanna et and bEk6d bv Hl€ltand Malavsla wllh nut llona conlonts in
al. (19811 isolated an active protoin (polypopiido"p) from Tab 6 1. 18 hoilthy ind vlduals (aga 22lo 40 yoals old, 3 mal€ and
seods bt acid ethanol extracllon, whlch dscrsasod 15 t€m6le) w€rs selootsd for lh6 studv. Thos€ Indlvlduals
bloodglucoso in STz-indlcod diab6tlc rats and increased volunle€fed lo bo h thls sludy and lhe proJsol ha! be€n approv€d
lhe glycMic enzymes aotivily. Polyp€ptid€-p has al6o by the Unlv€lslv Elhical Commltt€o. The lndlviduals qtore €xam nod
by a roglslercd physlclan and excuslon laclor.e wsrc ob6sa (Bodv
shown lo hav6 hypoglycemic etloct In iuvenil€ and mass inderBMl more lhan 30) and any pr$crlbsd medloatons
maturity-ons€t dlab€llc patlents (Khan na et al., 1 981 ), Panollsllast€d ov6rn ghl (10 h)alt$taklng dlnnor. Ony plaln waler
t'D?oBtudy has al6o shown thal bitier gourd extlact to wa, consumed durino faEllng pe od. Tho n€xl |nomhg, blood was
hav6 r€Flative €lfect on pancreatic P-Cells (Xiang ot al., ta€n ano basellno blood g uco€o lsv€l was n6a$r€d (bslorc
2004. Aparl from its hypoglycemic aclivily, bitler gourd corumpton ol bLr), Conlrols<strong>of</strong>l lll(2 x 35 o 6ach l.gredei as
also ha6 lipid loworing elfgct. S€rum and llver lipids, ln Tabl€ 1) was oonslm€d mm€dlaloly.
Blood 1,as tak€n at 30,90, 150 and 210 mln. Durlno lhlE tssl
especial! lriglyc€ride concentBtion was signiljcanlly peroo, only plan waler ls allo{eo lo bo consLmad All lhs
lowersd h €ts ted dists containing 1% bifter gourd orocodu'€s wero rsDoared ansr 24 h wnl. Ppk-supp6m€11€o solt
powder tompared to those f6d diels with no bitter gourd Roll (2 x 35 g saoh. IngGdl6nt as In Tablo 1) in ths samo
(Senana.lake et al., 2004). There is incroased intorest in
finding e nalurally occuffing, plant-based compound ' a Oata w6rc sxprossed 6 m66n I SD. Data ol bolh control and
functjond tood ingredieflt, which could prevent exp€ m€nlal was slbjeci€d to 2 way ANOVA Lrslng SPSS v 1s.
and/or Meanswilh p < 0.05 b comd€r€d signilica|lt.
amefDrale D[, (Schake et al., 2008)- On€ such lood
ingred€d is said to have this potenllal is polypoptide-k.
Polnepiide-k is a functional food ingredleni lhat is RESULTS AND DISCUSSION
exlraclsd from soeds ol bilt€r gourd. lt has high
homologt with human insulin and helps in rejuvenaling Figure 1 illustrate ths blood glucose lev6l lrom 0lo 30
pano€€6 and activatos inactive insulin. lt conlains 18 min, both control s<strong>of</strong>l roll and Ppk-<strong>supplemented</strong> s<strong>of</strong>t roll
siandard amino aclds in a single molecule and its caused ihcrement in blood glucose lev6l from baselins
appfcaton as a food ingredient may help in reducing glucose level by 1.5 mmoyl and 1.6 mmol/L respeclively.
blood glu.ose level and prevention <strong>of</strong> diabete6 (Khanna, Frcm 30 to 210 min, blood glucose l6vel for both control
2004L
and Ppk-suppl€menled sott rolls d€croassd. This showed
Thereforo, lhe objective <strong>of</strong> this currenl study was to that digestion had takon place where cadohydrale was
delenmln€ lhe eflect ol Polypeptide-k supplem€nted s<strong>of</strong>i bfoken into glucose and Insulin in blood has acted on the
roll ard control s<strong>of</strong>l roll on blood glucose level in heallhy glucose allowing the glucose to g€t absorbed inlo the
individuals.
cells. However, blood glucoss levelwilh <strong>polypeptide</strong> solt

Tabb r. Nut lional iniomation ol Polypeptide-k supplemsnlod andcontols<strong>of</strong>l lolls.
Lee et al
PDk-Suoll€menled s<strong>of</strong>t roll'ouantitv 061100 qm Conlrols<strong>of</strong>l roll'quantlty p6r 100 gm
Eneey 260.9 KCAL
268.9 KCAL
7.3 gm
4.9 gm
6-9 0m
4.9 gm
45.9 om
49.10m
lnE€dl€ris lEl rwhear
llour, war6', <strong>bun</strong>s., yoasl, wholo milk powd€r, pasleuized whoo oqg, bread imprcv6r, salt, slsvia
enrad, polypeprd6-l (ppl2 md.'zWhear llour, waler, sugar, blnor,lr€sh y€asl,lul cEam milk powder, pastourized whole
e!q, ascorbicacid (E300), Emursfler (E472e)
Tabls 2, Blood glucose levelol 18 panels wlth conlroland polyp€plid€-k supplem6nled Sott aolls at 0,30,90, 150 and 210 tnh
1
2
3
5
6
I
I
10
t1
12
t3
15
16
17
18
Averaqe blood glucoso (mmol/L)
Standad dovialion
4,4
4.3
5.6
5.4
6.2
4,9
5.4
5.0
5.2
5.2
4.8
5.0
4.7
5.4
5.4
5.3
5.1
0.46
roll .equir€d lesser time, 110 min lo drop lo baseline
glucose level as compared to control solt roll, which
required 190 min.
Paired samples tesl shows thai at 90, 150 and 210 min,
Ppk-supplemsnl€d soit roll give significant decrement
(p

1O lrtJ. Nulr. Metab.
While lorcontrol s<strong>of</strong>t rolls, only after 150 min, 100 % ot
the panels experienced drop in blood glucose. This is
becauso lhe pancreas will only respond to release il')sulin
when glucose is in excess in blood and thls account lor
the longer response time for blood glucose reduction
(Khanna el al., 1981). Rosults from the currsnl sludy
showed lhat Ppk in the <strong>supplemented</strong> s<strong>of</strong>t <strong>bun</strong>s
accelerated carbohydrale metabolism resulting in rapid
reduclionot glucose level as compared to control sott roll
in healthyadults.
ACKNO1VLEDGEMENTS
The aulh<strong>of</strong>s wish to thank [,,lagna Bio Laboratories and
Hiestand Malaysia Sdn. Bhd. lor sponsoring this panel
sludy.
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