pdf_file - Karol Korzekwa web page

PERSONAL INFORMATION
Name KORZEKWA KAROL, BRONISŁAW
Page 1 - Curriculum vitae of
[ Korzekwa Karol ]
Address 18/3 ORŁOWICZA STREET, 10-684, Olsztyn, Poland
Telephone / fax/ mail Portable: 0500 029 259 / (-) / idefix@moskit.uwm.edu.pl
Nationality / mother tongue Polish / Polish
Date of birth 06-09-1973
WORK EXPERIENCE
• Dates 1998 – to present
• Name and address of employer Department of Genetics, Faculty of Biology, Warmia And Mazury University in Olsztyn
• Type of business or sector Educational sector, High School
• Occupation or position held and
Main activities and responsibilities
1. Research works in range of man infectious diseases with special emphasis on tuberculosis
and lung diseases (molecular epidemiology and genetics) as main performer;
2. High school teacher in range of genetics, genetic engineering, genomics and nursing;
3. Laboratory main manager.
• 2003 NATO military training with promotion to second lieutenant (with excellent note);
• 2002 Military training in range of personnel and staff in Military High School of Poznań;
• 1998 - 1999 Reserve officers training in Military High School of Poznań - as tank and infantry platoon
commander (passed exams with good note);
• 1997 - 1998 Ecological fuel company - as projecting and existing facilities adviser and designer;
• 1996 3 months experience in Giżycko City District Office - as main inspector assistant (natural
resources management – wood and wastewater management – wastewater facilities);
• 1995 3 months experience in biggest tire factory STOMIL-OLSZTYN - as main engineer assistant
(estimating of gas, air and water pollution);
EDUCATION AND TRAINING
•(2005) PhD in biology, speciality of microbial genetics –
doctors thesis: “Application of DNA markers to genetic diversity estimation
in Mycobacterium tuberculosis strains”
•(2000 – 2004) PhD student in Dept. of Genetics, Faculty of Biology, Warmia And Mazury University in Olsztyn
•(2004) conference „Molecular epidemiology of infectious diseases on tuberculosis example” – Provincial Hospital of
Oncology and Lung Diseases in Olsztyn (as speaker);
•(2003) conference „Polish in European Research Space” –Education and Computerisation Ministry (as participant);
•(1999) conference „Micropollutants in human habitat” –Częstochowa Polytechnic in Ustroń (as speaker);
•(1999) conference „Gen-Probe as success in tuberculosis diagnosis” – Tuberculosis And Lung Diseases Institute in
Warsaw organised by bioMérieux (as participant);
•(1997) MSc in Environmental Protection, speciality water protection –
Master thesis: “Municipal wastewater treatment plant as a source of bioaerosols”
•(1993 - 1997) MSc student in Dept. of Water Microbiology, Faculty of Environmental Protection and Inland
Fishery, Agriculture and Technology Academy of Olsztyn (excellent note)”
LANGUAGES ENGLISH FRENCH RUSSIAN
• Reading skills excellent good good
• Writing skills very good good good
• Verbal skills good basic basic
SKILLS
AND COMPETENCES
1. Fluent in DOS environment, very good service of Windows 3.11, 95, 98 and XP. Fluent in
packet of Office 97, 2003 programmes (word, excel, access, power point) and . Very good
acquaintance of graphic programmes Corel and Dreamweaver. Fluent service: thermalcyclers,
spectrophotometers, microscopes, peristaltic pumps, centrifuges and autoclaves as well as
DNA techniques (isolation, quntification).
2. Computer graphic art, guitar music, mountain cycling, eastern arts of combat, archery, poetry.
For more information go to
idefix@moskit.uwm.edu.pl

DRIVING LICENCE
LIST OF PUBLICATIONS
AND SUMMARY
FUTURE EXPECTATIONS
EXPERIENCED WITH:
Page 2 - Curriculum vitae of
[ Korzekwa Karol ]
Yes – B category for cars since 1995, any accidents
1. Filipkowska Z., Korzekwa K., 1999. Municipal wastewater treatment plant in Olsten as a
bioaerosols source. Micropollutants in human environment. Częstochowa Polytechnic,
226–233;
2. Filipkowska Z., Korzekwa K., 1999. Municipal wastewater treatment plant as a source of
microorganisms polluting the air. Engineering and Environmental Protection, t.2 (3-4),
333–345;
summary: These papers present the results of the studies on the degree of pollution and the sanitary –
bacteriological conditions of the bioaerosols from wastewater treatment plant (flow rate about 60 000
m 3 /d). Microbiological analysis comprised he following: TVC (26°C and 37°C), hemolyzing bacteria
(37°C and 10°C), Enterobacteriaceae family, Staphylococcus and Pseudomonas strains,
actinomycetales and fungi (yeast and moulds) amounts cfu/m 3 . Aeration tanks and recirculated sludge
open channel are the major sources of the airborne bacteria. The number of hemolyzing bacteria above
50 cfu/m 3 indicated air pollution by pathogens around all technological objects situated in area of
wastewater treatment plant.
3. Polok K., Sawicki J., Kubiak K., Szczecińska M., Korzekwa K., Szandar K., Zieliński R., 2004.
Evolutionary divergence within Pellia endiviifolia (Dicks.) Dum. from Poland. Variability and
Evolution. Adam Mickiewicz University (in press)
summary: Three molecular forms of Pellia endiviifolia were identified in the Polish territory based on
semi-specific markers ISJ, that amplify nuclear DNA on the exon-intron junctions. The Nei’s genetic
similarity between the typical and water form of P. endiviifolia, was calculated on the base of ISJ
(I=0,183) and between them and the well-headed form (I=0.182 and 0.330, respectively). Taking into
consideration the molecular and morphological diversity, the evolutionary status of the well-headed form
as well as two early sibling species of P. endiviifolia can correspond to well defined biological species.
4. Polok K., Urbaniak L., Korzekwa K., Androsiuk A., Ciągło S., Kubiak K., Zieliński R., 2004.
Genetic similarity of Pinus sylvestris populations on the base of DNA markers. Variability and
Evolution. Adam Mickiewicz University (in press)
summary: Different types of DNA markers were used to estimate the extent of genetic similarity among
seven P. sylvestris populations that originated from different parts of Europe. Both genome scanning
markers, RAPD and ISJ, and the analysis of bacterial insertion element IS6110 was applied. Total of 71
bands was obtained and 63% of them were polymorphic. The similar proportion of polymorphic loci was
detected by ISJs and RAPDs, i.e. 71% and 75% respectively while IS6110 revealed only 27% of
polymorphic loci. The strongest discrimination of populations was displayed by RAPD markers, than by
ISJ markers and the lowest by IS6110. A certain degree of correlation between geographical distribution
of populations and their similarities was observed.
5. Polok K., Pisarek W., Urbaniak L., Korzekwa K., Zieliński R. 2004. Genetic characteristics of
Pinus sylvestris fo. turfosa Woerl from the Gązwa Reserve, North-Eastern Poland. Variability
and Evolution. Adam Mickiewicz University (in press)
summary: The present study was carried out to identify the genetic basis of the turfosa phenotype. In
total 55 trees of the turfosa form inhabiting the Gązwa peat-bog in Poland for variations in
dendrometrical, 7 morpho-anatomical characteristics of needles and in 3 types of DNA markers, i. e.
IS6110, ISJ and RAPD. The data demonstrated clearly the significant differences between turfosa and
typical P. sylvestris from the Gązwa Reserve. Reduction of DNA polymorphism was observed in the
turfosa form. It showed only 2% of polymorphic loci in comparison with 29% of polymorphic loci in typical
P. sylvestris. The unique genotype of the turfosa form was confirmed by 7 diagnostic loci. The present
data confirmed the genetic distinctiveness of the turfosa form and thus they may have implications with
respect to the conservation of this valuable form. In addition the great differences between turfosa and
typical P. sylvestris justifying the elevation of the former to a variety.
1. Population genetics of bacteria in relation to genetic diversity within and between populations;
2. Molecular epidemiology of human and cattle pathogens;
3. Virulence mechanisms of human and cattle pathogens;
4. Evolution of bacteria in wide range with little emphasis on virulence mechanisms
of human and cattle pathogenes and routs of archea evolution;
5. Microbiological wastewater treatment facilities monitoring;
6. Bioremediations and genetic modification of bacteria to wastewater,
sludge, gas and air utilization;
1. Mycobacterium tuberculosis;
2. E.coli;
3. Pseudomonas aeruginosa;
4. Pseudomonas fluorescens;
5. Aeromonas hydrofila;
For more information go to
idefix@moskit.uwm.edu.pl
6. Streptococcus faecalis, faecium and mitis;
7. Srteptococcus bovis, equinus and avium;
8. A little with other such like:
( Klebsiella, Enterobacter, Citrobacter, Bifidobacter,
Salmonella and Shigella)

PHD THESIS ABSTRACT
REFEREES:
Page 3 - Curriculum vitae of
[ Korzekwa Karol ]
Supplemental informations
The obligatory human pathogen, Mycobacterium tuberculosis ssp tuberculosis, is the
most important ethiological factor of tuberculosis. Unfortunately, there is a little information about
genetic diversity of this pathogen. The main aim of this research was the estimation of genetic
diversity of M. tuberculosis on the base of various categories of DNA markers.
Thirty two strains from patients including 25 susceptible and 3 isoniazid resistant, 2
streptomycin resistant and 2 isoniazid and streptomycin resistant were analysed. All strains were
isolated from patients from Warmia and Mazury region.
The genome scanning DNA markers such as RAPD and ISJ and insertion sequence
IS6110 as well as catalase-peroxidase katG gene were applied. Catalase-peroxidase gene was
divided into 12 fragments that were analysed separately.
One hundred and sixty one loci were identified, including 111 RAPD, 32 ISJ and 18
IS6110. All analysed loci were polymorphic. The genetic diversity coefficient, HS of M.
tuberculosis was relatively high for RAPD and ISJ markers and little bit lower for IS6110. There
were 14 alleles in katG gene. The level of genetic diversity was similar for susceptible and
resistant strains. There were no specific markers for each category of studied strains.
All strains were characterised by individual molecular pattern. Genetic similarity of
analysed strains varied from 0.13 to 0.94 for RAPD+ISJ markers and from 0 to 1 for insertion
sequence IS6110. The results showed that the analysed M. tuberculosis strains did not have
clonal structure, a single source of transmission and epidemiological relationships as well.
The applied DNA markers proved to be highly efficient for analysis of genetic structure
of M. tuberculosis and its epidemiological transmission routes.
Prof. Maria Krzakowa – PhD reviewer;
Adam Mickiewicz University in Poznań, Experimental Biology Institute,
Department of Genetics, Międzychodzka 5 street, 60-371 Poznań,
Tel. 061 8292722
mail: krzakowa@amu.edu.pl
Prof. Aleksander Świątecki – PhD reviewer;
University of Warmia and Mazury in Olsztyn, Faculty of Biology, Department of Microbiology,
M. Oczapowskiego 1A, 10-957 Olsztyn,
Tel. 089 5234428
mail: aswiat@matman.uwm.edu.pl
Prof. Roman Zieliński – PhD supeviser;
University of Warmia and Mazury in Olsztyn, Faculty of Biology, Department of Genetics,
Plac Lodzki 3 street, 10-967 Olsztyn,
Tel. 089 5234429
mail: rzielin@moskit.uwm.edu.pl
Prof. Lech Urbaniak – cooperator;
Adam Mickiewicz University in Poznań, Experimental Biology Institute,
Department of Genetics, Międzychodzka 5 street, 60-371 Poznań
Tel. 061 8292723
mail: carexu@amu.edu.pl
Prof. Stanisław Niewolak – past mentor and MSc reviewer,
University of Warmia and Mazury in Olsztyn, Faculty of Environmental Protection and
Fishery, Department of Environmental Microbiology, Oczapowskiego 2 street, 10-967
Olsztyn
Tel. 089 5234532
mail: zofiaf@uwm.edu.pl
Dr hab. Zofia Filipkowska – past mentor and MSc superviser,
University of Warmia and Mazury in Olsztyn, Faculty of Environmental Protection and
Fishery, Department of Environmental Microbiology, Oczapowskiego 2 street, 10-967
Olsztyn
Tel. 089 5234532
mail: zofiaf@uwm.edu.pl
For more information go to
idefix@moskit.uwm.edu.pl