Briefing Session on Analysis of Nitrate/Nitrite and ... - uri=govtlab.gov
Briefing Session on Analysis of Nitrate/Nitrite and ... - uri=govtlab.gov
Briefing Session on Analysis of Nitrate/Nitrite and ... - uri=govtlab.gov
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Nit <strong>Nitrate</strong>/<strong>Nitrite</strong>: t /Nit it Flow Fl Injecti<strong>on</strong> I j ti <strong>Analysis</strong> A l i C<strong>on</strong>diti<strong>on</strong> C diti<br />
Buffer / Carrier: Amm<strong>on</strong>ium chloride buffer soluti<strong>on</strong> (Carrier <strong>and</strong> Buffer):<br />
1. 53.2 g NH4Cl + 1800 mL distilled dei<strong>on</strong>ized water in a 2 L volumetric flask.<br />
22. Adj Adjust pH H to 8.5 8 5 ± 00.1 1 with i h 15N sodium di hydroxide h d id soluti<strong>on</strong> l i ddropwise. i<br />
3. Make up to the mark with distilled dei<strong>on</strong>ized water.<br />
Sulphanilamide/NED colour reagent:<br />
1. 100 mL 85% phosphoric acid + 40.0 g sulphanilamide + 1.0 g <strong>of</strong> NED in about 1L<br />
distilled dei<strong>on</strong>ized water.<br />
2. Store in a dark bottle.<br />
Washbath<br />
Dissolve 1 g <strong>of</strong> Tween 20 in 4 L distilled dei<strong>on</strong>ized water.<br />
Buffer + sample<br />
Carrier<br />
Sulphanilamide/<br />
NED colour reagent<br />
Dialysis Cell<br />
‐‐‐‐‐‐‐‐‐‐‐<br />
Cd Column<br />
Waste<br />
Detector<br />
540nm
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