Chromatofocusing with Polybuffer and PBE
Chromatofocusing with Polybuffer and PBE
Chromatofocusing with Polybuffer and PBE
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18-1009-07<br />
Edition AB<br />
<strong>Chromatofocusing</strong><br />
<strong>with</strong> <strong>Polybuffer</strong><br />
<strong>and</strong> <strong>PBE</strong><br />
H<strong>and</strong>book
Antibody Purification<br />
H<strong>and</strong>book<br />
18-1037-46<br />
The Recombinant Protein H<strong>and</strong>book<br />
Protein Amplification <strong>and</strong> Simple Purification<br />
18-1142-75<br />
Protein Purification<br />
H<strong>and</strong>book<br />
18-1132-29<br />
Ion Exchange Chromatography<br />
Principles <strong>and</strong> Methods<br />
18-1114-21<br />
Affinity Chromatography<br />
Principles <strong>and</strong> Methods<br />
18-1022-29<br />
Hydrophobic Interaction Chromatography<br />
Principles <strong>and</strong> Methods<br />
18-1020-90<br />
Gel Filtration<br />
Principles <strong>and</strong> Methods<br />
18-1022-18<br />
H<strong>and</strong>books<br />
from Amersham Pharmacia Biotech<br />
Reversed Phase Chromatography<br />
Principles <strong>and</strong> Methods<br />
18-1134-16<br />
Exp<strong>and</strong>ed Bed Adsorption<br />
Principles <strong>and</strong> Methods<br />
18-1124-26<br />
<strong>Chromatofocusing</strong><br />
<strong>with</strong> <strong>Polybuffer</strong> <strong>and</strong> <strong>PBE</strong><br />
18-1009-07<br />
Microcarrier cell culture<br />
Principles <strong>and</strong> Methods<br />
18-1140-62
<strong>Polybuffer</strong> TM <strong>and</strong> <strong>Polybuffer</strong> exchangers <strong>PBE</strong> 94 <strong>and</strong> <strong>PBE</strong> 118 have been specially<br />
designed by Amersham Pharmacia Biotech for chromatofocusing. They are<br />
inexpensive <strong>and</strong> very simple to use.<br />
This h<strong>and</strong>book is designed as an introduction to chromatofocusing <strong>and</strong> as a guide<br />
to the use of chromatofocusing products available from Amersham Pharmacia<br />
Biotech.
Fig. 1. Fractionation of soluble proteins extracted from elk muscle. Column C 10/40. Bed height: 35<br />
cm. Sample: 5 ml of supernatant from elk meat homogenate. Elution conditions: Start buffer 0.025 M<br />
ethanolamine HCl, pH 9.4, elution buffer 0.0075 mmol/ pH unit/ ml <strong>Polybuffer</strong> 96, pH 6. Flow rate: 20<br />
cm.h -1 .
Recorders REC 111, REC 112<br />
Reliable <strong>and</strong> accurate chart recorders for use <strong>with</strong> Pharmacia monitors or other<br />
laboratory equipment to give a permanent record of your experiments.
Correct column design is essential for the best results. Amersham Pharmacia Biotech<br />
provide a wide range of column designs to suit different applications <strong>and</strong> budgets.<br />
The best results are obtained on a long narrow column. Useful column types are<br />
the C10/40, SR 10/50, C16/70 <strong>and</strong> XK 16/70.
18-1110-91
<strong>Polybuffer</strong>, Pharmalyte, Sephadex <strong>and</strong> Sepharose are trademarks of Amersham Pharmacia Biotech Limited.<br />
Amersham is a trademark of Amersham plc.<br />
Pharmacia <strong>and</strong> Drop Design are trademarks of Pharmacia Corporation.<br />
All goods <strong>and</strong> services are sold subject to the terms <strong>and</strong> conditions of sale of the company <strong>with</strong>in<br />
the Amersham Pharmacia Biotech group that supplies them.<br />
A copy of these terms <strong>and</strong> conditions is available on request.<br />
© Amersham Pharmacia Biotech AB 2001 – All rights reserved.<br />
Amersham Pharmacia Biotech AB Björkgatan 30, SE-751 84 Uppsala, Sweden<br />
Amersham Pharmacia Biotech UK Limited Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, Engl<strong>and</strong><br />
Amersham Pharmacia Biotech Inc 800 Centennial Avenue, PO Box 1327, Piscataway, NJ 08855 USA<br />
Amersham Pharmacia Biotech Europe GmbH Munzinger Strasse 9, D-79111 Freiburg, Germany<br />
Amersham Pharmacia Biotech KK, Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan
www.apbiotech.com<br />
TK i Uppsala AB 2001
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