Real-Time qPCR Kits with EvaGreen - Jena Bioscience
Real-Time qPCR Kits with EvaGreen - Jena Bioscience
Real-Time qPCR Kits with EvaGreen - Jena Bioscience
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<strong>Real</strong>-<strong>Time</strong> <strong>qPCR</strong> <strong>Kits</strong> <strong>with</strong> EVAGreen TM<br />
<strong>EvaGreen</strong> TM Fluorescent DNA Stain is a superior intercalator dye specially<br />
developed for real-time <strong>qPCR</strong> applications including high-resolution DNA<br />
melting curve analysis. <strong>EvaGreen</strong>TM is highly stable both thermally<br />
and hydrolytically, resulting in minimal quenching. Combined <strong>with</strong> its high<br />
quantum yield and low interference <strong>with</strong> PCR it is the ideal fluorophore<br />
for real-time PCR and a superior and convenient replacement for commonly<br />
used dyes such as SYBR ® Green I.<br />
Simply select the optical<br />
setting for SYBR ® Green or FAM<br />
Excitation and emission spectra of <strong>EvaGreen</strong> TM is similar<br />
to SYBR ® Green / FAM. Excitation max: λ Ex =500 nm,<br />
Emission max: λ Em =530 nm(<strong>EvaGreen</strong> TM bound to dsDNA<br />
in PBS buffer pH 7.3)<br />
<strong>EvaGreen</strong> TM<br />
Comp. Dye<br />
extra peak<br />
80 85 90 80 85 90<br />
DNA melting curve analysis using <strong>EvaGreen</strong> TM and<br />
Competitor Dye <strong>with</strong> 4 different amplicons. Competitor Dye<br />
shows occasional formation of an extra melting peak.<br />
SYBR ® Green I <strong>EvaGreen</strong> TM<br />
Degeneration of SYBR ® Green I <strong>with</strong>in 3 hours at 99°C.<br />
<strong>EvaGreen</strong> TM shows no detectable decrease in fluorescence<br />
intensity. (Each fluorophore 1.2 µM in Tris-HCl buffer pH 9.0)<br />
PCR amplification plots using <strong>EvaGreen</strong> TM and<br />
Competitor Dye at two different concentrations.<br />
Competitor Dye exhibits significant PCR inhibition<br />
at 1× concentration while <strong>EvaGreen</strong> TM does not.<br />
Our <strong>EvaGreen</strong>TM <strong>qPCR</strong> product series provides easy-to-handle and powerful tools for quantification of sample DNA in a broad<br />
dynamic range of up to 6 orders of magnitude <strong>with</strong> exceptional sensitivity and precision. The high specificity and sensitivity of our<br />
<strong>EvaGreen</strong>TM reagents is complemented by an optimized hot-start polymerase preventing extension of non-specifically annealed<br />
primers or primer-dimer formations at low temperatures during PCR setup.<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH<br />
Loebstedter Str. 80<br />
07749 <strong>Jena</strong><br />
Germany<br />
High-resolution DNA<br />
melting curve analysis<br />
<strong>Jena</strong> <strong>Bioscience</strong> introduced a new system for quantitative real-time PCR based on<br />
the fluorescent DNA stain <strong>EvaGreen</strong>TM . The green fluorescent dye enables rapid<br />
analysis of any target DNA <strong>with</strong>out additional sequence-specific DNA probes.<br />
<strong>EvaGreen</strong>TM is compatible <strong>with</strong> all common <strong>Real</strong>-<strong>Time</strong> PCR cyclers – simply select<br />
the standard settings for SYBR ® Green or FAM!<br />
Phone +49 (0)3641- 62 85 000<br />
Fax +49 (0)3641- 62 85 100<br />
www.jenabioscience.com<br />
info@jenabioscience.com<br />
Relative Fluorescence<br />
Delta Rn<br />
<strong>EvaGreen</strong> TM is<br />
highly stable<br />
Delta Rn vs. Cycle<br />
Cycle number<br />
Wavelength (nm) Cycle number<br />
Relative Fluorescence<br />
Low interference<br />
<strong>with</strong> PCR
Please contact us for inquiries and<br />
further information or request your<br />
customized quotation.<br />
<strong>Real</strong>-<strong>Time</strong> <strong>qPCR</strong> <strong>Kits</strong> <strong>with</strong> EVAGreen TM<br />
<strong>qPCR</strong> Ready-to-Use Mixes <strong>with</strong> Green Fluorescent DNA Stain<br />
<strong>qPCR</strong> GreenMaster <strong>with</strong> UNG / ROX<br />
<strong>qPCR</strong> GreenMaster <strong>with</strong> UNG<br />
<strong>qPCR</strong> GreenMaster <strong>with</strong> ROX<br />
<strong>qPCR</strong> GreenMaster<br />
<strong>qPCR</strong> Core <strong>Kits</strong> <strong>with</strong> Green Fluorescent DNA Stain<br />
<strong>qPCR</strong> Green Core Kit <strong>with</strong> ROX<br />
<strong>qPCR</strong> Green Core Kit<br />
<strong>qPCR</strong> Supplements<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH<br />
Loebstedter Str. 80<br />
07749 <strong>Jena</strong><br />
Germany<br />
Phone +49 (0)3641- 62 85 000<br />
Fax +49 (0)3641- 62 85 100<br />
www.jenabioscience.com<br />
info@jenabioscience.com<br />
PCR-304S 100 reactions 130,– Euro<br />
PCR-304L 500 reactions 520,– Euro<br />
PCR-303S 100 reactions 130,– Euro<br />
PCR-303L 500 reactions 520,– Euro<br />
PCR-314S 100 reactions 110,– Euro<br />
PCR-314L 500 reactions 440,– Euro<br />
PCR-313S 100 reactions 110,– Euro<br />
PCR-313L 500 reactions 440,– Euro<br />
PCR-334S 100 reactions 90,– €<br />
PCR-334L 500 reactions 360,– €<br />
PCR-333S 100 reactions 90,– €<br />
PCR-333L 500 reactions 360,– €<br />
ROX Reference Dye PCR-351 500 reactions 25,– €<br />
<strong>EvaGreen</strong> TM Fluorescent DNA Stain PCR-352 500 reactions 40,– €<br />
Thermolabile UNG PCR-353 200 units 100,– €<br />
<strong>qPCR</strong> Control Kit PCR-354 500 reactions 130,– €<br />
<strong>EvaGreen</strong> TM is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.<br />
SYBR ® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.<br />
Find out more details at our website<br />
www.jenabioscience.com
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