Properties of Fluorescent Dyes and Quenchers 1 - Jena Bioscience
Properties of Fluorescent Dyes and Quenchers 1 - Jena Bioscience
Properties of Fluorescent Dyes and Quenchers 1 - Jena Bioscience
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<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
<strong>Fluorescent</strong><br />
Color<br />
Near-IR DY-776<br />
Near-IR DY-751<br />
Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
λem<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
835 771 801 120 pure<br />
815 751 779 220 pure<br />
Near-IR ATTO 740 undisclosed 568 740 764 120 pure<br />
Near-IR ATTO 725 undisclosed 516 725 752 120 pure<br />
Isomers Notes/<br />
Advantages<br />
Soluble in water,<br />
negatively charged,<br />
spectrum similar to LICOR<br />
IRD 800RS<br />
Red ATTO 700 undisclosed 575 700 719 120 pure Substitute for Cy5.5<br />
Red ATTO 680 undisclosed 631 680 700 125 pure<br />
Red ATTO 665 undisclosed 623 663 684 160 pure<br />
Red ATTO 655 undisclosed 634 663 684 125 pure<br />
Red OYSTER-656<br />
737 656 674 200 pure<br />
Red Cy5 658 649 670 250 pure<br />
Red ATTO 647N undisclosed 746 644 669 150 two<br />
Red ATTO 633 undisclosed 652 629 657 130 pure<br />
Substitute for Cy5.5;<br />
spectroscopic properties<br />
similar to LICOR IRD 700<br />
Suitable for STED<br />
applications<br />
Substitute for Cy5, but<br />
better since no triplet<br />
behavior<br />
Higher photostability<br />
compared to Cy5<br />
Substitute for Alexa Fluor<br />
647 <strong>and</strong> Cy5; base<br />
stable<br />
Emission maximum <strong>of</strong> He-<br />
Ne laser, base stable,<br />
less hydrophilic<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
<strong>Fluorescent</strong><br />
Color<br />
Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
λem<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
Red ATTO Rho14 undisclosed 784 625 646 120 pure<br />
Red ATTO 620 undisclosed 612 619 643 120 pure<br />
Red DY-480XL<br />
515 500 630 50 pure<br />
Red ATTO 594 undisclosed 1137 601 627 120 pure<br />
Orange ATTO Rho13 undisclosed 646 600 625 125 pure<br />
Orange ATTO 590<br />
691 594 624 120 two<br />
Orange ATTO Rho101 undisclosed 590 586 610 120 pure<br />
Orange Texas Red<br />
Orange ATTO Thio12<br />
725 588 609 80 pure<br />
503 579 609 110 pure<br />
Orange ATTO Rho12 undisclosed 650 576 601 120 pure<br />
Isomers Notes/<br />
Advantages<br />
Substitute for LightCycler<br />
Red 640; base stable<br />
Large Stokes Shift <strong>of</strong><br />
130 nm<br />
Substitute for Alexa Fluor<br />
594, more hydrophilic<br />
than ATTO 590<br />
Substitute for Texas Red,<br />
Alexa Fluor 594<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
<strong>Fluorescent</strong><br />
Color<br />
Orange 6-ROX<br />
Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
λem<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
535 575 600 82 pure<br />
Orange ATTO Rho11 undisclosed 567 571 595 120 pure<br />
Orange ATTO 565<br />
611 563 592 120 two<br />
Yellow ATTO 550 undisclosed 694 554 576 120<br />
Isomers Notes/<br />
Advantages<br />
three<br />
60%:22%:17%<br />
Yellow 5/6-TAMRA 430 545 575 90 two<br />
Yellow Cy3 632 550 570 150 pure<br />
Yellow-<br />
Green<br />
Yellow-<br />
Green<br />
ATTO Rho6G undisclosed 514 535 560 115 pure<br />
DY-485XL 503 485 560 50 pure<br />
Substitute for Cy3, Alexa<br />
Fluor 546, Bodipy<br />
558/568; better<br />
photostability<br />
Substitute for Cy3, Alexa<br />
Fluor 546, Bodipy 558/<br />
568; better photostability<br />
Large Stokes Shift <strong>of</strong><br />
75 nm<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
<strong>Fluorescent</strong><br />
Color<br />
Yellow-<br />
Green<br />
Yellow-<br />
Green<br />
Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
ATTO 532<br />
6-JOE<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
λem<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
765 532 553 115 pure<br />
Isomers Notes/<br />
Advantages<br />
Substitute for Alexa Fluor<br />
532; Bodipy 530/550<br />
504 520 548 75 pure Substitute for VIC, HEX<br />
Green ATTO 495 452 495 527 80 pure<br />
Green ATTO 488 804 501 523 90 pure<br />
Green 6-FAM<br />
Green 5-FAM<br />
376 492 517 83 pure<br />
376 492 517 83 pure<br />
Substitute for FITC, Alexa<br />
Fluor 488, Cy2; identical<br />
to Alexa, much more<br />
photostable than Cy2 or<br />
FITC<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
<strong>Fluorescent</strong><br />
Color<br />
Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
λem<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
Isomers Notes/<br />
Advantages<br />
Blue-Green ATTO 465 396 453 508 75 pure Substitute for Cyan 500<br />
Blue-Green ATTO 425<br />
401 436 484 45 pure<br />
Blue ATTO 390 343 390 479 24 pure<br />
Blue MANT<br />
151 335 440 2,7 pure<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
<strong>Properties</strong> <strong>of</strong> <strong>Fluorescent</strong> <strong>Dyes</strong><br />
<strong>and</strong> <strong>Quenchers</strong><br />
Quencher Structure MW<br />
(free acid)<br />
[g/mol]<br />
DYQ-661<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
λabs<br />
[nm]<br />
Quenching<br />
Range<br />
[nm]<br />
771 661 610 - 680 pure<br />
DYQ-660 669 660 610 - 690 pure<br />
ATTO 612Q undisclosed 791 615 570 - 660 two<br />
ATTO 580Q undisclosed 795 586 540 - 610 three<br />
ATTO 540Q undisclosed 659 542 490 - 570 two<br />
Redox Dye Structure MW<br />
(free acid)<br />
[g/mol]<br />
λabs<br />
[nm]<br />
εmax<br />
[cm -1 *M -1 ]<br />
ATTO MB2 284 658 110 pure<br />
Isomers Recommended in<br />
combination with<br />
dye...<br />
DY-630, -480XL; ATTO-<br />
620, -635, -647(N),<br />
-655; OYSTER-656<br />
DY-630, -480XL; ATTO-<br />
620, -635, -647(N)<br />
-655; OYSTER-656<br />
ATTO 610, -620; -635,<br />
-647(N)<br />
TAMRA; ATTO 550, -<br />
565, -590; ROX<br />
FAM; ATTO 488, -495,<br />
-532; JOE; DY-485XL<br />
Isomers Notes/<br />
Advantages<br />
Reversible reducible to<br />
its colorless leuko-form<br />
http://www.jenabioscience.com Last update: Apr 19, 2012
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