Ch. 28 Vibrio, Aeromonas, Plesiomonas, and Chromobacterium ...
Ch. 28 Vibrio, Aeromonas, Plesiomonas, and Chromobacterium ...
Ch. 28 Vibrio, Aeromonas, Plesiomonas, and Chromobacterium ...
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臨床微生物學 (Clinical microbiology )<br />
<strong>Ch</strong>. <strong>28</strong><br />
<strong>Vibrio</strong>, <strong>Aeromonas</strong>, <strong>Plesiomonas</strong>, <strong>and</strong><br />
<strong>Ch</strong>romobacterium<br />
MacConkey-positive<br />
Oxidase-positive<br />
吳雪霞 吳雪霞 老師<br />
老師<br />
醫學檢驗暨生物技術學系<br />
醫學檢驗暨生物技術學系<br />
分機 分機:3322 分機 :3322<br />
1
學習目標<br />
• 1. 使同學能明白臨床微生物實驗室及臨床<br />
微生物醫檢師所扮演的角色。<br />
• 2. 使同學能熟悉臨床微生物相關各菌種的<br />
特性及其致病機制。<br />
2
參考資料<br />
• 1. Bailey & Scott's Diagnostic Microbiology<br />
• 2. Manule of Clinical Microbiology<br />
3
glucose-fermenter<br />
4
Wound infection<br />
5
Rice water<br />
6
<strong>Vibrio</strong>static agent 0/129<br />
Biotypes: classical <strong>and</strong> El-Tor<br />
classical El-Tor<br />
<strong>Ch</strong>icken <strong>and</strong> sheep RBC agglutination - +<br />
Hemolysis on BAP - +<br />
Polymyxin B susceptibility S R<br />
Mukerjee phage susceptibility S R<br />
10
V. cholerae:<br />
Cary-Blair medium (but not buffered glycerol saline)<br />
Alkaline peptone water (pH8.4), 5-8hr<br />
13
Thiosulfate-citrate-bile salts-sucrose<br />
14
Indole DNase<br />
+ +<br />
- -<br />
+ -<br />
16
0.5% sodium deoxycholate<br />
17
<strong>Ch</strong>. 29<br />
Sphingomonas paucimobilus <strong>and</strong><br />
Similar Organisms<br />
MacConkey-negative<br />
Oxidase-positive<br />
19
<strong>Ch</strong>. 30<br />
Moraxella<br />
MacConkey-negative<br />
Oxidase-positive
U penicillin disk<br />
nglucose utilizer (except N. elongata subsp glycolytica)
Liquefy serum
<strong>Ch</strong>. 31<br />
Eikenella <strong>and</strong> Similar Organisms<br />
MacConkey-negative<br />
Oxidase-positive
ubacute bacterial endocarditis<br />
Haemophilus aphrophilus ch.34<br />
Actinobacillus actinomycetemcomitans ch.33<br />
Cardiobacterium hominis ch.33<br />
Eikenella corrodens ch.31<br />
Kingella kingae ch.33
Eikenella corrodens<br />
(ch.31)<br />
Sta. intermedius<br />
CDC group EF-4a,4b<br />
Bergeyella zoohelcum
MTM<br />
thylobacterium spp: 25 ℃, Utilize acetate
<strong>Ch</strong>. 32<br />
Pasteurella <strong>and</strong> Similar Organisms<br />
MacConkey-negative<br />
Oxidase-positive
se fermenter
Eikenella corrodens<br />
(ch.31)<br />
Sta. intermedius<br />
CDC group EF-4a,4b<br />
Bergeyella zoohelcum
<strong>Ch</strong>. 33<br />
Actinobacillus, Kingella,<br />
Cardiobacterium, Capnocytophaga,<br />
<strong>and</strong> Similar Organisms<br />
MacConkey-negative<br />
Oxidase-positive
ubacute bacterial endocarditis<br />
Haemophilus aphrophilus ch.34<br />
Actinobacillus actinomycetemcomitans ch.33<br />
Cardiobacterium hominis ch.33<br />
Eikenella corrodens ch.31<br />
Kingella kingae ch.33
GLC
<strong>Ch</strong>. 34<br />
Haemophilus<br />
MacConkey-negative<br />
Oxidase-variable
Hemin (X)
ypeable <strong>and</strong> nontypeable<br />
apsule
ubacute bacterial endocarditis<br />
Haemophilus aphrophilus ch.34<br />
Actinobacillus actinomycetemcomitans ch.33<br />
Cardiobacterium hominis ch.33<br />
Eikenella corrodens ch.31<br />
Kingella kingae ch.33
fluenzae: Horse blood-bacitracin agar for CF patient
Sucrose<br />
-<br />
+
Summary<br />
1. Introduction of the importance of clinical<br />
microbiology lab <strong>and</strong> how to establish it.<br />
2. Introduction of the characteristics <strong>and</strong><br />
the pathogenesis of each microorganisms.
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