English - McKnight Foundation Collaborative Crop Research Program
English - McKnight Foundation Collaborative Crop Research Program
English - McKnight Foundation Collaborative Crop Research Program
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Edited by:<br />
A M Mbwaga ARI Uyole, Tanzania<br />
Joseph Hella Sokoine University of Agriculture, Tanzania<br />
J. Mligo Ilonga <strong>Research</strong> Institute, Tanzania<br />
V. Kabambe Bunda College University of Malawi<br />
September 2009<br />
1<br />
2009
Project Team<br />
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Institution Name<br />
Tanzania<br />
Uyole Agricultural <strong>Research</strong> Institute, PO<br />
Box 400, Mbeya<br />
Ilonga Agricultural <strong>Research</strong> Institute, PO<br />
Box 33, Kilosa<br />
Sokoine University of Agriculture<br />
PO Box 3007, Morogoro<br />
Dodoma Rural District Council<br />
PO Box 1089, Dodoma<br />
Dr A M Mbwaga (Pathologist & Project Leader)<br />
~ Dr J K Mligo (Cowpea breeder)<br />
~ M. Mchomvu (Home Economics)<br />
~ Dr Joseph Hella (Agric. Economist)<br />
~ G. Martin (MSc Student)<br />
~ D. Muywanga (District Agricultural and Livestock<br />
Officer)<br />
~ Ms Mwamba (Subject Matter specialist)<br />
Singida Rural District Council ~ B. Manento (DALDO)<br />
~ L. Sakwera (<strong>Crop</strong>s specialist)<br />
Iringa Rural District Council<br />
PO Box 290<br />
INADES Formation, Tanzania<br />
PO Box 203, Dodoma<br />
Bunda College, University of Malawi, PO<br />
Box 219, Lilongwe<br />
Chitedze <strong>Research</strong> Station<br />
PO Box 158, Lilongwe<br />
Department of <strong>Crop</strong> Production, Ministry of<br />
Agriculture. PO Box 1035, Capital City,<br />
Lilongwe<br />
International Institute of Tropical<br />
Agriculture. C/O Lambourne Ltd, 26<br />
Dingwall Rd, Croydon CR9 3EE, UK<br />
~ P. Mphwewe (DALDO)<br />
~ Mrs Nyalusi (<strong>Crop</strong>s Specialist)<br />
A. Katunzi (Director & Training specialist)<br />
Malawi<br />
~ Dr V Kabambe (Weed Scientist, Senior Lecturer &<br />
project coordinator for Malawi)<br />
~ T Chilongo (Agricultural Economist)<br />
~ Dr J. Bokosi, (Plant Breeder)<br />
~ Dr A Mangwela (Food scientist)<br />
~ Ms Kazila. (MSc Student)<br />
~ William Henderson Harawa<br />
~ BSc Student in Agricultural Economics<br />
~ Mr E Mazuma (Pathologist & National Commodity Team<br />
Leader, Pulse <strong>Research</strong>)<br />
~ Ms C Mtambo. Entomologist and Chief <strong>Crop</strong> Protection<br />
Officer<br />
Nigeria<br />
Boukar Ousmane (Breeder) IITA Kano Nigeria<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Table of Contents<br />
Page<br />
Summary VI<br />
Introduction 2<br />
Project objectives 2<br />
Summary of research activities 2<br />
Objective 1. Development of high yielding A. vogelii resistant cowpea varieties 2<br />
Objective 2. Promotion of high yielding A. vogelii resistant cowpea varieties 13<br />
Implications of the research findings 15<br />
Annex 1 Tanzania Country Report 17<br />
Annex 2: Cowpea Utilization in Iringa and Dodoma Regions, Tanzania 23<br />
Annex3: Malawi Country Report 32<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Summary:<br />
The parasitic weed Alectra vogelii is a wide spread constraint to cowpea production in semi-arid areas<br />
of Southern Africa, where the crop is an important source of protein for resource poor farmers. A<br />
project aiming to develop and promote Alectra resistant cowpea cultivars was initiated in Malawi and<br />
Tanzania. Farmer research groups have been established in cowpea growing areas of both countries<br />
and have been focusing on evaluation of cowpea lines for adaptation, yield, resistance to Alectra and<br />
processing and utilization of cowpea grain for three seasons now. Initial studies of farmers and market<br />
trader’s preferences in both countries Malawi and Tanzania indicate that large white to-cream seed<br />
types are most favored. Growers also need plant types that produce copious foliage for spinach and for<br />
preservation for consumption in the dry season. Cowpea germplasm collected from local institutions,<br />
farmers and the breeding program at the International Institute of Agriculture (IITA) Nigeria was<br />
evaluated. In Tanzania this season farmers have selected from five promising to three lines for their<br />
yield performance, Alectra resistance and earliness. Selected cowpea lines included IT99K-7-21-2-2-1,<br />
IT99K-573-1 and TZA 263. These lines are now being multiplied at Ilonga Agricultural <strong>Research</strong><br />
Institute for planting by more farmers on a large scale next season.In Malawi line IT99K-7-21-2-2-1<br />
was selected the best in terms of Alectra resistance, yield and grain size and is being earmarked for<br />
release. Parallel to this work some of these materials have been analyzed for macro and micro-nutrient<br />
content at Sokoine University of Agriculture Tanzania (see next section below). Post-graduate student<br />
projects on production, utilization and marketing of cowpea were completed in Tanzania last year, but<br />
there are still to be completed at Bunda College in Malawi.<br />
The processing of cowpea especially into grain was found to be a laborious work for women. Using<br />
tradition method of preparing - for example buns (Bagia) - a woman had to sleep late and to wake up<br />
around four in the morning ready to prepare Bagia. After introduction of new ways of preparing flour<br />
women are now it takes less than an hour to prepare Bagia, hence there is a lot of time saving. This can<br />
enable each member of the family to prepare Bagia for the children each morning. It has also been<br />
observed that dehuled grain can store for a long time without getting attacked by bruchids. The dehuled<br />
grain also cooks fast compared to grain. This is hoped to increased usage of cowpea grain at family<br />
level.<br />
Studies on micronutrients included also the frequency uptake of cowpea products by farmers in<br />
sampled villages of Iringa and Dodoma Tanzania Minerals of nutritional importance that were<br />
analyzed included iron, zinc and calcium; results indicated that, there was twice much calcium in leaves<br />
than in cowpea grains and almost 40 times as much iron. Since iron and zinc levels in cowpea leaves<br />
have shown to be significantly higher than the levels in cowpea grains, creating more awareness and<br />
encouraging the utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies<br />
from cheap sources especially from resource-poor families. The variety that was best with regards to<br />
all minerals was IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and<br />
fats content were IT97K499‐8 and IT99K‐721‐2‐2‐1.<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Introduction:<br />
Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural households in<br />
the drier and sub-humid region of Southern Africa, where diets tend to be overly reliant on starchy foods<br />
such as millet, sorghum, maize and cassava. The addition of even a small amount of cowpea ensures the<br />
nutritional balance of the diet and enhances the protein quality by the synergistic effect of high protein<br />
and high lysine from cowpea and high methionine and high energy from cereals. Hence cowpea grain is<br />
an inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />
particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />
harvest also it adds cash to the household after selling the grain and dried leaves. It has to be noted that<br />
cowpea is mainly a women crop.<br />
On-farm cowpea yields are extremely low, averaging 319 kg/ha in Tanzania and 388 kg/ha in Malawi.<br />
Use of late maturing cultivars, low plant density and insect damage are widely recognized as important<br />
constraints to improved cowpea production under on-farm conditions. Less well appreciated is the<br />
importance of the parasitic weed Alectra vogelii, which attaches itself to the roots of cowpea plants and<br />
interferes with the plants' ability to obtain water and nutrients. Recently-released improved cowpea<br />
cultivars that are earlier maturing and more tolerant to key insect pests and diseases are especially<br />
susceptible to Alectra attack, experiencing up to 50% yield reductions. A. vogelii is widespread from the<br />
Northern Province of South Africa, through Central Africa to Kenya and across West Africa to Mali. In<br />
Tanzania, A. vogelii is common in Mwanza, Shinyanga, Dodoma, Iringa, Mtwara and Ruvuma regions,<br />
while in Malawi, it is common in Lilongwe, Dowa and districts in central Malawi, the lower lying, drier<br />
areas of the southern region and the Blantyre/Shire Highlands<br />
Project objectives<br />
The general objective of the project is to improve cowpea productivity on A. vogelii-infested land in<br />
Malawi and Tanzania by introducing Alectra resistance into cowpea cultivars that are also early<br />
maturing, pest/disease tolerant and high yielding. The specific objectives are to develop high yielding A.<br />
vogelii resistant cowpea cultivars and then to promote them in both countries, Malawi and Tanzania.<br />
SUMMARY OF RESEARCH ACTIVITIES:<br />
Objective 1: Development of high yielding Alectra vogelii resistant cowpea varieties<br />
In year three of the project further studies have been undertaken to consolidate breeding through transfer<br />
of resistance to Alectra from the resistant lines (donor parents) into the commercial released varieties<br />
(recurrent parents) which are susceptible to Alectra by using a backcross method to improve the<br />
released varieties both in Malawi and Tanzania. Farmers groups established continued to be<br />
strengthened through training and participation in joint activities both within and outside their villages.<br />
Farmers in study villages continued to conduct on farm evaluation of the promising cowpea lines with<br />
commercially released varieties as checks. This year, 10 promising lines in Tanzania and five lines in<br />
Malawi were evaluated by farmers based on their own suitability criterion. In year three, project team<br />
continued to develop partnerships with farmer groups thus allowing continued on farm trials and<br />
availing useful data to research for making informed decisions in on-station breeding programmes.<br />
Through exchange visits and joint design of cowpea breeding programme, research collaboration<br />
between Malawi and Tanzania was increased and capacity was enhanced through farmer group training<br />
and graduate (BSc. and MSc.) students training. Details of activities undertaken on the outputs that the<br />
project had planned to address during the 2008/09 season (year 3) are presented in the individual county<br />
reports in the Annexes. A highlight of this work is as follows<br />
Output 1.1: Producer and consumer preference in cowpea identified<br />
The activities under this output were completed in year 1 and 2 and are found in annual report of 2008.<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />
In Tanzania farmer groups have expanded from 6 to 8 and emphasis of the training was on the new<br />
farmer groups. Similarly two farmer groups were added in Malawi. The training given to the new farmer<br />
groups was on group formation, leadership and writing a group constitution. Training was also<br />
conducted on seed production using demonstration plots.<br />
Participatory Video shooting was one of the new activities introduced to farmers this season starting in<br />
Tanzania. Two farmer groups from Kikombo village (12farmers) in Dodoma and Mangalali village (14<br />
farmers) in Iringa region participated in the participatory video shooting training course. , The outcome<br />
has shown to be very effective in empowering farmers to document activities they are undertaking in the<br />
project. Their participation at CoP3 in Bagamoyo Tanzania was very evident and has created a<br />
motivation for them to undertake more of these documentation of project activities.<br />
Output 1.3: Cowpea lines resistant to A vogelii identified<br />
Assembling of cowpea germplasm:<br />
The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />
obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />
Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />
starting in the same year one. In Malawi the germplasm used came from IITA and from their national<br />
collection and Chitedzi <strong>Research</strong> Institute.<br />
On-station screening of cowpea cultivars for Alectra resistance<br />
In Tanzania, this activity started in 2007 growing season and the promising lines were tested to more<br />
Alectra infested sites. This year the trials were planted at three on-station locations which included<br />
Ismani, Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those<br />
from last season showing promising Alectra resistance and farmers had already selected some of them<br />
as the best lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288,<br />
TZA 263, IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207 and B301. VULI-2 and FAHARI<br />
were included as local checks. The materials were planted at a plot size of 3m x 4m, 4 rows per plot and<br />
replicated four times. Data recorded included; plant stand count at harvest, Alectra count per plot and<br />
yield per plot (kg/plot). Yield data was computed to yield in kg per ha.<br />
Generally weather condition was not very good for the crop, because rains started late and were erratic.<br />
Dodoma suffered the most for all the crops and this affected the crop establishment and Alectra<br />
infestation. From the test cowpea materials, only cowpea line IT97K-499-38 and B301 supported zero<br />
Alectra counts at three locations. (Table1). Other lines supported low Alectra plants as compared to the<br />
susceptible checks (Vuli-2 and Fahari) or TZA 263, and IT99K-573-2-1.The susceptible varieties<br />
(released commercial varieties) are being improved by incorporating resistance to Alectra by backcross<br />
method. At present the work is at backcross three progressing into backcross four, this will be followed<br />
by selfing then screening for resistance to Alectra.<br />
Table 1: Average infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />
locations at 12 weeks after planting 2009:<br />
Cowpea Entries Ismani Bihawana Hombolo Mean Comments<br />
FC<br />
Alectra<br />
count/sites<br />
IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of<br />
Alectra<br />
IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of<br />
Alectra<br />
IT97K-499-8 0.0 0.5 0.0 0.2<br />
IT97K-818-35 0.0 1.0 0.0 0.3<br />
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IT97K-819-118 0.0 4.5 0.0 1.5<br />
IT97K-499-38 0.0 0.0 0.0 0.0<br />
IT89KD-288 0.0 26.3 0.0 8.8<br />
IT00K-1207 0.8 1.8 0.0 0.9<br />
IT96D-733 0.0 42.0 0.0 14.0<br />
B301 0.0 0.0 0.0 0.0<br />
TZA 263 0.0 1.5 0.0 0.5 Farmers selected<br />
for large grain<br />
Vuli 2 3.0 48.3 0.3 17.2 Commercial<br />
released varieties<br />
Fahari 7.5 34.3 1.0 14.3 Commercial<br />
released varieties<br />
Mean 0.87 12.73 0.15 7.58<br />
SE 0.41 2.98 -<br />
Range: 0-16 0-74 0-3<br />
In Malawi five of entries selected for further testing based on Alectra resistance, farmer preferences on<br />
grain characteristics and according to evaluations conducted in the last two years were planted at<br />
Chitedze and Bunda College. The trial’ design was complete block design, with 4 replicates. The trial<br />
plots had 5 rows, 4 m long and 0.75 m apart. Other traits evaluated included: grain yields and yield<br />
components (e.g. seed per pod,), days to flowering and maturity and disease incidences.<br />
Screening for Alectra resistance, entries IT 97K-825-15; IT99K-7-21-2-2-1 and IT 99K-494-6 recorded<br />
least number of Alectra counts (Table 2). Entries IT99K-494-6 (1369kg/ha), IT82E-16 (1786kg/ha) and<br />
Sudan 1(1827kg/ha) recorded high yield and the same entries showed larger number of seeds per pod<br />
than other entries<br />
Table 2: Alectra counts m -2 taken after flowering at all the trial sites in Malawi<br />
Entrycode & name Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />
IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />
IT97K-825-15 0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />
T99K-7-21-2-2-1 0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />
IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />
Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />
IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />
Farmers’ local 2.80 1.14 3.29 - 0.03 0.11 1.23<br />
Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot.<br />
With assistance from Village Extension Officers farmers were able to collect data and evaluate the<br />
materials based on their own set criterion.<br />
As discussed earlier, rainfall distribution was a major problem. It started late and erratic; hence there<br />
was very poor crop establishment especially in Iringa and Dodoma region. Singida received well<br />
distributed rainfall but there was a serious outbreak of cut worms on legumes in that region. The Alectra<br />
infestation was only reported on commercially released varieties and local cultivars, the other lines were<br />
observed free from the parasitic weed. The average highest cowpea yield was recorded from Singida<br />
region followed by Iringa and last Dodoma as presented in Table 3.<br />
Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania<br />
2009<br />
Cowpea line Farmer group managed to harvest from their plots of cowpea<br />
Mkungugu Iringa Kikombo Dodoma Msungua Singida<br />
IT99K-7-21-2-2-1 600 222 600<br />
IT99K 573-2-1 720 440 600<br />
IT97K 818-35 200 389 800<br />
IT89KD 288 400 333 200<br />
TZA 263 280 167 1200<br />
VULI-2 560 - 600<br />
FAHARI 800 - 760<br />
Village Local- 200 - 530<br />
Mean 470.0 310.2 661.3<br />
On-farm evaluation of cow pea lines (responses from farmers)<br />
Results of the on farm evaluation of selected cultivars was conducted in five participating villages;<br />
Msungua and IKhanoda (Singida region), Kikombo (Dodoma region), Mkungugu and Mangalali (Iringa<br />
region) based on the criterion they identified during the 2008 growing season i.e. resistance to Alectra,<br />
diseases/insects/pests, high yielding, large white to cream coloured seed, early maturity, production of<br />
many and tender leaves, drought resistance. These criteria were matched with five lines presented in<br />
Table 4.<br />
Table 4: Five best lines selected in 2008 evaluation session<br />
Lines Line description based on 2008 selection<br />
IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding, white<br />
coloured seed,<br />
IT 99K 573-1-1 Slightly late maturing, resistant to Alectra, average seed size, good seed colour,<br />
high yielding<br />
1T 97K 818-35 Late maturity, resistant to Alectra<br />
1T 89KD 288 Late maturity, high yielding, resistant to Alectra, normal seed colour,<br />
TZA 263 Late maturity, resistant to Alectra, good colour, large seeded<br />
Results indicated that line TZA263, followed by IT 99 K-7-21-2-2-1 and IT 99K-573-1-1 suited<br />
farmers’ social, economic and cultural interest. Based on these findings, lines TZA 263, IT 99K-7-21-2-<br />
2-1 and IT 99K-573-1-1 are to be increased for distribution to many farmers for further evaluation in<br />
2010 season. Initial process to establish descriptors for these lines for possible inclusion in the National<br />
Performance Trials (NPT) towards variety releases will be initiated.<br />
On farm evaluation in Malawi was conducted in Riviri, Bunda College, Mngwangwa, Mpokwa<br />
Khuvinda village, Mpokwa Chibisa village, and Lisasadzi. Seven entries involving four lines IT98K-<br />
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503-1, IT97K-825-15, IT99K-7-21-2-2-1, IT99K-494-6, and two released varieties Sudan -1, IT82E-16<br />
and one farmers’ local cultivar were evaluated against five main criteria, which are; seed size, plant<br />
type, early maturity, insect resistance and disease resistance. Note that varieties Sudan -1 and IT82E-16<br />
were included for counterchecking with introduced lines. Preliminary description of the lines is<br />
presented in Table 5.<br />
Table 5: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />
Code Name Description<br />
5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra vogelii<br />
resistance<br />
37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />
preference<br />
9 IT99K-7-21-2-2- Best Alectra resistance in ’07, ‘07/08, good seed size, medium seed size,<br />
1<br />
good yield<br />
21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium seed size,<br />
low farmer preference<br />
53 Sudan -1 Released variety, good resistance to Alectra and good yielder in ‘07/08<br />
52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08. Poor<br />
resistance to A. vogelii. Well liked by farmers.<br />
54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />
Results of analysis show preference on the following order. Sudan 1 is most preferred followed by<br />
IT82E-16, IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6. Lines IT97K-825-15 followed by<br />
farmers’ local are less preferred as indicated above. Since Sudan 1 and IT82E-16 are already released,<br />
varieties IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6 may be considered for further genetic<br />
perfection on-station with a possibility of releasing them commercially starting with line IT99K-7-21-2-<br />
2-1.<br />
Geographic variability in host response to A. vogelii<br />
Studies undertaken in pot experiment at Long Ashton UK indicate that at a species level there<br />
are 3 strains of Alectra by host range:<br />
1. Alectra from Singida attaches and emerges on cowpea and groundnut but not on mung or common<br />
bean.<br />
2. Alectra from Bihawana and probably Ismani attaches and emerges on cowpea, groundnut, common<br />
bean but not on mung bean<br />
3. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and mung<br />
bean.<br />
Within cowpea, patterns of virulence were also observed to be evident: Alectra from all sites in both<br />
countries emerges on IT36E-16 and TZA 263 but not on B301.<br />
1. Alectra from Bunda and Kasungu emerges on ITK1207, IT97K 818-35, IT97K819-118 and IT<br />
99K7-21-2-2-1<br />
2. Alectra from Zomba emerges on ITK1207, IT97K 818-35, IT97K819-118 but not on IT 99K7-21-<br />
2-2-1 (this result for IT 99K7-21-2-2-1 same as in 2008 pot trial).<br />
3. Alectra from Singida and Ismani does not emerge on ITK1207, IT97K 818-35, IT97K819-118 and<br />
IT 99K7-21-2-2-1 (result for Singida same as in 2008 trial)<br />
4. Alectra from Bihawana emerges on IT 99K7-21-2-2-1 but not on ITK1207, IT97K 818-35 and<br />
IT97K819-118.<br />
In Tanzania it appears that Alectra from Singida has a narrower species host range and less virulent on<br />
the cowpea lines tested than Alectra from other sites. Bihawana Alectra provides a "universal" test for<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
the three Tanzania sites. From this study the cowpeas that are resistant at Bihawana are also resistant at<br />
Singida and Ismani.<br />
In Malawi it appears that Alectra from Bunda and Kasungu are similar but different to Zomba.<br />
It has to be also noted that the Alectra samples we have from Malawi are different from the parasite<br />
collected further south near Blantyre as in trials some time back at Long Ashton this attacked B301<br />
(Mainjeni, 1999).<br />
It has been more confusing to find the result for IT81D-994 as this was resistant to all samples in 2008.<br />
There must be something went wrong with the samples delivered to Long Ashton this year.<br />
The most reliable method for characterization of these strains would be to go for using molecular<br />
markers.<br />
Reference:<br />
Mainjeni, C. E. (1999) The host range of Alectra vogelii Benth. From Malawi and resistance in common<br />
bean and cowpea. Msc. Thesis, University of Bath, UK. pp 83.<br />
Table 6: Response of 11 cowpea, one mung bean, one bambara groundnut and two groundnut lines to<br />
five accessions of A.vogelii.<br />
Alectra accession<br />
Zomba Malawi (ex cowpea) Bunda Malawi (ex groundnut) Kasungu Malawi (ex groundnut)<br />
Line Emerged No of<br />
reps<br />
Cowpea<br />
Un‐<br />
emerged<br />
Emerged No. of<br />
reps<br />
10<br />
Un‐<br />
emerged<br />
Emerged No. of<br />
reps<br />
2009<br />
Un‐<br />
emerged<br />
IT86E‐16 19.3 + 9.7 3 3 19.3 + 17.5 3 3 20.3 + 4.6 3 3<br />
B301 0 0 2 0 0 3 0 2 0 1<br />
TZA‐263 19 + 8.7 3 3 14.7 + 7.3 3 3 8.3 + 5.9 3 3<br />
IT00K‐1207 2.3 + 1.9 2 3 9 + 2.1 3 3 5.7 + 3.2 2 3<br />
IT81D‐994 1.3 + 0.7 2 3 1.3 + 0.9 2 3 0.3 + 0.3 1 3<br />
IT97K‐818‐35 1.3 + 1.3 1 3 2.7 + 1.8 2 3 3.3 + 2.8 2 2<br />
IT97K‐819‐118 0.7 + 0.7 1 3 3.3 + 2.4 2 3 1 + 0.6 2 2<br />
IT99K‐7‐21‐2‐2‐<br />
1<br />
Common bean<br />
0 0 3 0.7 + 0.2 2 3 0.7 + 0.3 2 3<br />
Kabalabala 1.7 + 1.7 1 NR 0 0 NR 2.7 + 2.2 2 NR<br />
Maluwa 4.3 + 2.2 2 3 3 + 0.8 3 3 4 + 2.5 3 3<br />
Masaka 1.0 + 0.6 2 3 5 + 4 2 3 3 + 1.7 2 3<br />
Mung bean<br />
Ex Tanzania 1.7 + 1.7 1 2 4 + 0.8 3 3 2.0 + 0.6 2 3<br />
Groundnut<br />
Chalimbana 0.7 + 0.7 1 NR 0.3 + 0.3 1 NR 1.3 + 1.3 1 NR<br />
CG7 2 0.5 + 0.4 1 NR 1.7 + 0.9 2 NR 2 + 1 2 NR<br />
1 Only one replicate pot established. 2 Only two replicate pots established, NR = not recorded yet.<br />
Table 6 continued.<br />
Singida Tanzania (ex cowpea)<br />
Alectra accession<br />
Bihawana Tanzania (ex cowpea) Ismani Tanzania (cowpea)<br />
Line Emerged No of Un‐ Emerged No. of Un‐ Emerged No. of Un‐<br />
Cowpea<br />
reps emerged<br />
reps emerged<br />
reps emerged<br />
IT86E‐16 13.3 +<br />
3.5<br />
3 3 9.3 + 6.8 3 3 3 + 0 3 3
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
B301 0 0 0 0 0 0 0 0 0<br />
TZA‐263 3.3 + 1.5 3 3 0.7 + 0.3 2 3 0.3 + 0.3 1 2<br />
IT00K‐1207 0 0 0 0 0 0 0 0 0<br />
IT81D‐994 0 0 1 2 2<br />
1 1 0.3 + 0.3 1<br />
IT97K‐818‐<br />
35<br />
0 0 2 0 0 1 0 0 1<br />
IT97K‐819‐<br />
118<br />
0 0 1 0 0 2 0 0 0<br />
IT99K‐7‐21‐<br />
2‐2‐1<br />
Common<br />
bean<br />
0 0 2<br />
0.7 + 0.3<br />
2<br />
2 0 0 1<br />
Kabalabala 0 0 NR 0 0 NR 0 0 NR<br />
Maluwa 0 0 2 1.3 + 0.3 3 3 0.3 + 0.3 1 2<br />
Masaka<br />
Mung bean<br />
0 0 3 2 + 1 3 3 0.3 + 0.3 1 2<br />
Ex Tanzania<br />
Groundnut<br />
0 0 0 0 0 0 0 0 0<br />
Chalimbana 0.7 + 0.7 1 NR 0 0 NR 0 0 NR<br />
CG7 0 0 NR 0 0 NR 0 0 NR<br />
NB: Mean number emerged stems per pot (+ S.E.) at 97 days after sowing. For entries with no emerged<br />
A. vogelii, the number of replicate pots with unemerged parasite attachments is shown. No standard<br />
errors are shown when there were less than three pots per cowpea/A. vogelii combination.<br />
Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />
Project activities are leading to increased capacity in cowpea research in Malawi and Tanzania and the<br />
strengthening of collaboration between institutions in both countries. Within the project, two researchers<br />
Abubakar Mzanda from Tanzania and Dr Vernon Kabambe from Malawi participated in a week long<br />
statistics training course offered by <strong>McKnight</strong> <strong>Foundation</strong> at Centre for Agricultural <strong>Research</strong><br />
Development (CARD)Bunda College University of Malawi June 15-22, 2009.<br />
• Two project staff – Drs J.P. Hella and V Kabamabe attended ASARECA review workshop on<br />
climate change. The workshop was held at Naivasha Kenya.<br />
• A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />
Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual<br />
Agricultural Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in<br />
AGREST conference series Vol. 8. (in press). The proceedings will be ready within and outside<br />
the country. Mr Gabriel Martin’s got financial support from the project for his MSc research work<br />
• Group dynamics training was conducted for all the farmers in the two participating villages in<br />
Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />
leadership and operation. This group strengthening included membership and responsibilities,<br />
constitution, networking and fund raising to sustain the groups.<br />
• During the season an undergraduate student (Ms Elida Kazira )undertook a study on cowpea time<br />
of planting x variety x density trial titled - Farmer Participatory Selection of Alectra Resistant<br />
Cowpea Varieties in Selected Districts of Malawi). The student has submitted her thesis for<br />
examination.<br />
• Participatory video workshop was held at INADES Dodoma, where two extension staff from the<br />
projects sites Dodoma and Iringa respectively participated and these also went back and trained<br />
farmers from the groups at Mangalali Iringa (14 farmers) and Kikombo in Dodoma (12 farmers)<br />
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Output 1.5: Project Monitored and evaluated<br />
To accomplish Activity 1.5.2 (Review progress against M & E criteria each year) monitoring and<br />
evaluation activities were conducted in Tanzania and Malawi as planned. The objective was to evaluate<br />
on going on-farm and on station cowpea project activities and crossing work in screen house.<br />
The monitoring and evaluation for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro)<br />
from 27 th to 30 th April, 2009 with participants including Ambonesigwe Mbwaga (Project Leader) ARI,<br />
Uyole Mbeya Tanzania, Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania; Vernon Kabambe (Weed<br />
specialist) Bunda College, Lilongwe, Malawi; James Bokosi (Breeder) Bunda College, Lilongwe,<br />
Malawi; Boukar Ousmane (Breeder,) IITA Nigeria. On-farm visits were done in regions of Iringa and<br />
Dodoma, whereas on-station visits were done at Ismani (Iringa region), Bihawana (Dodoma region and<br />
at Ilonga research institute (Morogoro region). Crossing for cowpea varieties to improve resistance to<br />
Alectra is being conducted.<br />
In Malawi, monitoring and evaluation was conducted from 15 th to 20 th March 2009. The team was<br />
composed of Drs Joseph Mligo (cowpea breeder), Ambonesigwe Mbwaga (Project team leader), Charlie<br />
Riches (CoP Liaison Officer) and Malawian counterparts comprising Dr Vernon Kabambe (Country<br />
project leader), Mosses Mamiliro (Breeder, and James Bokosi (Breeder). For On –farm activities 7<br />
cowpea cultivars of which 5 were experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-<br />
2-2-1, IT 97K 825-15, Bunda 1, IT98K503-1) one commercially released variety (IT 82E-16) were<br />
monitored in Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu district in<br />
Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA, Also seed<br />
multiplication of experimental cowpea lines such as TZA 263 (from Tanzania) and IT 81D-994. Seed<br />
increase also included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and<br />
IT82E-16.<br />
For on-station activities, the team visited Bunda college experimental site where seed multiplication of<br />
experimental cowpea lines which have shown Alectra resistance was being conducted. The<br />
performances of the materials were good and were at flowering stage in addition to seed multiplication<br />
activities at Bunda.<br />
Overall M&E team observed the following<br />
• Weather variability (especially drought) is an overriding problem for cowpea production. The<br />
team recommended the collaboration with the Tropical Legumes II project of IITA and try to<br />
incorporate both traits (Alectra resistance and drought tolerance) in the current breeding<br />
programme.<br />
• Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least<br />
preferred by the farmers. At all sites farmer’s variety was the latest, and it had not even<br />
flowered when all the other entries were maturing.<br />
• It was noted that only early maturing varieties were most preferred, forgetting the advantage of<br />
late maturing varieties for not having other traits such as prolonged availability of leaves as<br />
relish. This suggests that there is a need to evaluate the materials according to maturity groups.<br />
• The good production practices should be incorporated in the screening of the varieties against<br />
Alectra. Hence, there is need to expose the groups involved in the project to all these production<br />
practices.<br />
• There is a need to evaluate the materials according to maturity group: early, medium and late<br />
because farmers’ selections are dictated and influenced by time to maturity<br />
• The backcrossing programme at Ilonga will continue to backcross 6 by October 2010, while at<br />
Bunda will be at back cross 4. Request for extension of the project should be initiated early<br />
2010<br />
Overall the team noted good progress which are being made and high enthusiasm among stakeholders<br />
especially farmers who are conducting on-farm demonstrations<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Objective 2: Promotion of high yielding A. vogelii resistant cowpea varieties<br />
With the continuation of the on-farm trials/demonstration component of the project efforts to increase<br />
awareness of the problem of A. vogelii and approaches to management continued in year 3. Work<br />
implemented included farmers field exchange visits, participation in national agricultural shows, training<br />
on preparation of different cowpea recipes and some market studies in Malawi<br />
Output 2.1: Farmer and extension awareness of .A. vogelii and use of resistant cultivars enhanced<br />
In the implementation of the above mentioned output, two activities were conducted in Tanzania, these are<br />
farmer field days and exchange visits and participation in 2009 National Agricultural show. With respect to<br />
exchange visit, twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu (Fahari research<br />
group) and later both visited Ismani On-station trials in Iringa. The objective of the farmer exchange visit<br />
was for farmers from Mangalali to share experiences with other farmers on principles of cowpea<br />
production, processing and utilization and to be acquainted with on station cowpea lines screening work. At<br />
the research station farmers participated in evaluation of 14 cowpea screening materials planted. Four<br />
cowpea farmers of the CCRP project from Singida rural district (two from Msungua and two from<br />
Ikhanoda) were sponsored by their district council and attended the National Agricultural Show between 1 st<br />
and 8 th August 2009 in Dodoma. Promising cowpea lines and associate processed products of cowpea from<br />
their group were displayed.<br />
In Malawi, farmers from Mngwangwa EPA, on their own accord, organized themselves and participated at<br />
a District Agriculture Fair held in the same EPA in June 2009. The farmers demonstrated a range of<br />
cowpea products based on the training made by the project and shared this with participants. In addition,<br />
the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science Department. Again,<br />
the focus of the exhibit was on utilization. The exhibits attracted a lot of attention from participants<br />
Output 2.2a: Nutritional status of farmers improved<br />
To accomplish output 2.2 above, activity 2.2.2: (Develop/modify/ demonstrate cowpea products) was<br />
undertaken. The primary objective was to promote the utilization of cowpea in Tanzania. Specifically was<br />
to train farmers on the processing of cowpea grains into dhal, preparation of cowpea dishes (snack bites<br />
from cowpea flour “bagia’); and to perform sensory evaluation and organoleptic tests. The training was<br />
conducted in eight villages namely Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />
Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The villages were<br />
purposively selected as a result of their involvement in a project on “Improvement of cowpea productivity<br />
on Alectra Vogelii infested land in Tanzania. A one day workshop was conducted in each project village<br />
and farmers were trained on improved cowpea technologies. The trainings were based on demonstrations,<br />
hands-on trials and printed materials (brochures for cowpea recipes). Farmers participated in the validation<br />
of five recipes for cowpea value addition. Sensory and organoleptic tests were carried out, where by<br />
farmers were required to rank and find out the most preferred types of ”bagia”. The five types of bagia<br />
were made using different recipes. The recipes were translated into Swahili language for better<br />
understanding by farmers.<br />
Table 7: Cowpea snack product recipes<br />
Type of<br />
Materials/ingredients<br />
Bagia<br />
1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />
2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />
3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and water<br />
4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and water<br />
5. Bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs, yeast and<br />
water<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
In Malawi where farmers already have advanced knowledge on preparation of different<br />
cowpea/groundnut recipes, in areas where cowpea seed was provided, farmers were encouraged to use<br />
their cowpea to practice the different recipes which they learned from the project<br />
B: Cowpea Utilization in Iringa and Dodoma Regions<br />
Per capital consumption of 40‐200gms of cowpea grains were found to be 84% in Iringa and 94% in<br />
Dodoma, while per capital consumption for cowpea leaves (10‐500 grams) were 95% in Iringa and<br />
100% in Dodoma, respectively. More than 50% of households in Iringa and Dodoma consumed cowpea<br />
once to three times a week. The leaves are consumed fresh especially during wet season and in dried<br />
form during lean/dry season. Almost all farmers in the two regions consume cowpea leaves<br />
Macro‐ and micro‐ element analysis<br />
Analysis of the improved cowpea cultivars showed relatively higher protein levels of 24% to 26% and<br />
8% to 11% fat content. Similarly the improved cowpeas were relatively high in calcium, iron, and zinc.<br />
However, considerable mineral variation was found among the analyzed cowpeas varieties.<br />
Surprisingly, local cowpea cultivar had comparable high mineral calcium, zinc and iron concentrations<br />
compared with the improved varieties. This shows that apart from other merits that the improved<br />
cowpeas might have over local varieties such as higher yielding and resistance to certain diseases and<br />
pests, still nutritionally they are equally good. (See Annex 2)<br />
Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />
seed identified<br />
The seed availability is a big issue because commercial companies are not interested in producing seeds<br />
of low volume crops like sorghum, pearl millet bambara nuts and cowpea. To accomplish Activity 2.3.2<br />
{Train farmers to produce seed (QDS) for commercial use}, farmers were given basic training in<br />
community seed production covering four main topics namely Organization of seed production in<br />
Tanzania (L 1), Attributes of seed quality (L 2), Cowpea Seed Production (L 3) touching base on issues<br />
related to site selection for cowpea seed production, land preparation, choice of varieties to be planted<br />
according to days to maturity, and plant spacing depending on variety growth habit, crop husbandry<br />
practices such as pest management, rouging of off types, harvesting, seed conditioning, seed storage and<br />
seed sampling and testing for quality attributes. Table 8 present numbers of participants to on-farm seed<br />
production<br />
Table 8:. Participants to on-farm seed production for project participating farmers<br />
Region District Village Nane of<br />
No. of Topics Covered<br />
<strong>Research</strong> Group participants L1 L2 L3<br />
SINGIDA Singida Msungua Mshikamano 10 X X X<br />
rural Ikhanoda Mfwenji 20 X X X<br />
Nduu Matumaini 21 X X X<br />
DODOMA Dodoma Kikombo Ushirika 11 X X<br />
rural Mpunguzi Twende na<br />
wakati<br />
10 X X<br />
IRINGA Iringa Mkungugu Ari mpya 10 X X<br />
rural Mangalali Fahari 14 X X<br />
In Malawi, the project provided small packs of seed of 0.5 kg each to all project participating farmers at<br />
Mpokwa, Mngwangwa EPA (about 60 farmers at each EPA) and Kasungu. The objective was to create<br />
awareness on cowpea production. Subsequently, a follow-up training was conducted on pest<br />
management, to grow cowpea intensively (as sole crop) and to practice pest management and observe<br />
14<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
results. Farmers were monitored and they managed to get good crops and yields and were motivated to<br />
produce cowpeas. These farmers have been advised to store some of their seeds so that they can plant<br />
more cowpeas next season. They were also trained on principles of cowpea seed production during field<br />
days and visits.<br />
Output 2.4: Cowpea marketing opportunity identified<br />
In Malawi, cowpea market value chain workshops which brought representatives of cowpea producers,<br />
middlemen and traders were conducted in Lilongwe and Zomba in August 2009. The workshops were<br />
held to establish the linkages between cowpea producers (farmers) with the markets and to identify<br />
increased marketing opportunities. The workshop revealed that there are six big business traders who<br />
buy cowpea seeds (ADMARC, Rab Processors, Mulli Brothers, Agora, Grain Traders Association, and<br />
Transglobe) most of them paced emphasis on large seeded grain types as preferences.<br />
In Tanzania, the workshop was not held due to poor cowpea yields as a result of poor rainfall; hence<br />
farmers had nothing to offer to the traders. It is scheduled for May 2009 in Dodoma<br />
A paper - Gabriel, M. M.; Hella, J.P. and Mbwaga, A.M (2008) Assessment of Cowpea Marketing<br />
Efficiency: A case study of selected regions in Tanzania” will appear in the forthcoming AGREST<br />
conference proceedings Vol. 8 which is in press. Plan is underway to publish a paper in an international<br />
Journal for wider circulation. This is the first paper on cowpea marketing published in Tanzania<br />
Implication of the research findings<br />
During the past year, the project partners have built on work begun in year 1 and 2 to strengthen farmer<br />
groups, establish on farm trials and demonstrations, to take forward a cowpea-breeding programme,<br />
cowpea marketing stakeholders and to finalize marketing studies for Malawi. Crossing programme to<br />
incorporate resistance to Alectra into released commercial varieties has reached backcross 3 going in<br />
backcross 4 in Tanzania and backcross 2 in Malawi (due to delayed start in Malawi) These<br />
achievements will allow the project to move forward with next stage of the agreed work plan to<br />
undertake activities in the following ways;<br />
1. The project continues to support capacity building of farmer groups especially those new ones so<br />
that they are well informed about the aims of the project. Providing short term benefits for group<br />
membership should encourage continued participation in trials and other project activities.<br />
2. Crossing programme will continue up to backcross 6 by November 2010 in Tanzania, while in<br />
Malawi will be at backcross 4 because of late start.<br />
3. Wide scaling out identified Alectra resistant cowpea lines for Tanzania are three lines<br />
TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 and in Malawi are entries IT97K-825-15,<br />
IT99K-494-6 and IT99K-7-21-2-2-1<br />
4. Market studies have been completed in Tanzania and a stakeholders meeting will be conducted next<br />
financial year and in Malawi data is being analysed, a separate report will be prepared. Preliminary<br />
results were shared during the value chain workshops held in Lilongwe and Zomba respectively<br />
5. Farmer groups continued to be equipped with knowledge on QDS production and eventually. It is<br />
envisaged that QDS seed be bought by the project and then re-packed and farmers be given each<br />
member of the group give five new farmers of his choice to produce grain which can then be<br />
marketed by the group with aim to increase volume of grain production and hence increase grain for<br />
market at the same time popularize the varieties to more farmers.<br />
6. Since iron and zinc levels in cowpea leaves have shown to be significantly higher than the levels in<br />
cowpea grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
contribute in alleviating micronutrient deficiencies from cheap sources especially for resource-poor<br />
families.<br />
7. The new line, which was best with regards to all minerals, was IT99K-7-21-2-2-1 while the best<br />
with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1. The later has<br />
been selected by farmers for its earliness in both countries Tanzania and Malawi (Item 3 above). It<br />
will be one of the potential candidate to be promoted for release in both countries Malawi and<br />
Tanzania<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Annex 1: Tanzania Annual Report<br />
Development and promotion of Alectra resistant cowpea cultivars for smallholder farmers in<br />
Malawi and Tanzania<br />
Edited by:<br />
Dr Ambonesigwe M Mbwaga: Uyole Agricultural <strong>Research</strong> Institute, Box 400 Mbeya<br />
Dr Joseph Mligo: Ilonga Agricultural <strong>Research</strong> Institute, Box 33 Kilosa, Morogoro<br />
Dr Joseph Hella: Sokoine University of Agriculture, Morogoro<br />
September 2009<br />
1<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Tanzania Project Team<br />
Uyole Agricultural <strong>Research</strong> Institute Dr A M Mbwaga (Pathologist & Project Leader)<br />
PO Box 400, Mbeya<br />
Ilonga Agricultural <strong>Research</strong> Institute Dr J K Mligo (Cowpea breeder)<br />
PO Box 33, Kilosa<br />
Dodoma Rural District Council<br />
D Muywanga (District Agricultural and Livestock<br />
PO Box 1089, Dodoma<br />
Officer)<br />
Ms Stella Mwamba (Subject Matter specialist)<br />
Singida Rural District Council N Mosha (DALDO)<br />
L Sakwera (<strong>Crop</strong>s specialist)<br />
Iringa Rural District Council<br />
P Mphwewe (<strong>Crop</strong> specialist)<br />
PO Box 290<br />
INADES Formation, Tanzania<br />
A. Katunzi (Director & Training specialist)<br />
PO Box 203, Dodoma<br />
TOSCI P.O BOX 1056, Morogoro Mwakitwange (Seed Technologist)<br />
Photo: Cowpea Seed production plot at Mpunguzi village in Dodoma, Tanzania 2009<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Introduction:<br />
The rainfall amount and distribution during the 2008/09 season was poor in most dry areas of Tanzania.<br />
Also there was an outbreak of cut warms at seedling stage especially in Singida, where farmers had to<br />
re-plant legumes more than twice until they ran out of seed. This has led to poor harvest of the crop. The<br />
two outputs 1.1 and 1.2 mentioned below were covered in year one and year 2.<br />
Output 1.1: Producer and consumer preferences for traits in cowpea identified<br />
This out put has been completed in year two<br />
Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />
Three new more farmer groups were formed this season, one from each district of Singida, Dodoma and<br />
Iringa. These are from Nduu village (21 members) in Singida, Mpunguzi (10 members) in Dodoma and<br />
Ilambilole (8 members) in Iringa. Some preliminary training has been undertaken, mainly group<br />
formation, seed production and processing and utilization of cowpea.<br />
Output1.3.0: Cowpea lines resistant to A. vogelii identified<br />
1.3.1: To assemble cowpea germplasm:<br />
The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />
obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />
Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />
starting in the same year one.<br />
1.3.2 On-station screening of cowpea cultivars for Alectra resistance<br />
Introduction: Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural<br />
households in the drier and sub-humid regions of Eastern and Southern Africa, where diets tend to be<br />
overly reliant on starchy foods such as millet, sorghum, maize and cassava. Cowpea grain is an<br />
inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />
particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />
harvest, also it adds cash to the household after selling the grain, dried leaves and cookies like bagia<br />
(buns). Farmers grow local long duration cowpea cultivars, which produce low yield due to a number of<br />
reasons including Alectra infestation. In odder to improve the productivity of cowpeas in these areas,<br />
there was a need to introduce cowpea cultivars, which are high yielding, resistant to pests including<br />
Alectra and preferred by consumers both for market and for household use. The introduction of<br />
accessions was to asses for their resistance to Alectra. This activity started by this project in 2006/2007<br />
growing season and the promising lines were tested to more Alectra infested sites. These sites were<br />
Ismani in Iringa region; Bihawana Farmer Training Centre and Hombolo <strong>Research</strong> station both in<br />
Dodoma. For the second season 2007/2008 the cowpea lines were again planted at the same sites of<br />
Ismani, Bihawana FC and Hombolo to confirm their performance in terms of Alectra resistance and<br />
yield. Promising lines for Alectra resistance and yield were identified. In the third season 2008/2009, the<br />
promising lines were planted again at these Alectra hot spots for further confirmation of resistance to<br />
Alectra and for yield performance.<br />
Material and methods: The trial was planted at three on station locations which included Ismani,<br />
Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those from last<br />
season showing promising Alectra resistance and farmers had already selected some of them as the best<br />
lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288, TZA 263,<br />
IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207, B301 and VULI-2, FAHARI as local<br />
checks. The materials were planted at a plot size of 3x4m, 4 rows per plot and replicated four times.<br />
Data recorded were plant stand count at harvest, Alectra count per plot and yield per plot (g/plot). Yield<br />
data was computed to yield in kg per ha.<br />
3<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Results and discussions: Generally weather condition was not very good for the crop, because rains<br />
started late and erratic. Dodoma suffered the most for all the crops and this affected the crop<br />
establishment and Alectra infestation. The trial at Hombolo was mistakenly planted at a site where<br />
almost there was no Alectra infestation, only few Alectra plants were observed on susceptible cowpea<br />
checks Vuli-2 and Fahari. Looking at the susceptible checks high infestation of Alectra was observed at<br />
Bihawana Training Centre followed by Ismani. From the test cowpea materials only cowpea line<br />
IT97K-499-38 and B301 supported zero Alectra counts at both<br />
locations Ismani and Bihawana (Table 3.1). Other lines supported very low Alectra plants as compared<br />
to the susceptible checks Vuli-2 and Fahari.<br />
4<br />
2009<br />
Fig. 1 Cowpea field highly infested by Alectra at<br />
Bihawana Farmers training Centre Dodoma,<br />
2009<br />
Cowpea yield on average was observed highest at Hombolo followed by Bihawana FC and last was at<br />
Ismani. From the test cowpea lines, line IT99K-573-2-1 produced the highest average yield<br />
(735.6kg/ha) across sites followed by line IT97K-819-118 (647.3kg/ha). These lines also gave the<br />
highest yield from the test cowpea lines in 2008 season (Project Annual report 2008). Test line TZA 263<br />
was severely infected with a bacterial disease called bacterial blight especially at Ismani and Hombolo;<br />
in some of the plots almost all plants died due to the disease<br />
Fig. 2 Cowpea line TZA 263 attacked by a bacterial<br />
disease likely to be Bacterial blight (Xanthomonas<br />
vignicola)
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Table 1.Average Infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />
locations at 12 weeks after planting 2009:<br />
Cowpea Entries Ismani Bihawana<br />
FC<br />
Hombolo Mean Alectra<br />
count across<br />
5<br />
sites<br />
Comments<br />
IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of Alectra<br />
IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of Alectra<br />
IT97K-499-8 0.0 0.5 0.0 0.2<br />
IT97K-818-35 0.0 1.0 0.0 0.3<br />
IT97K-819-118 0.0 4.5 0.0 1.5<br />
IT97K-499-38 0.0 0.0 0.0 0.0<br />
IT89KD-288 0.0 26.3 0.0 8.8<br />
IT00K-1207 0.8 1.8 0.0 0.9<br />
IT96D-733 0.0 42.0 0.0 14.0<br />
B301 0.0 0.0 0.0 0.0<br />
TZA 263 0.0 1.5 0.0 0.5 Farmers selected for large<br />
grain<br />
Vuli 2 3.0 48.3 0.3 17.2 Commercial released varieties<br />
Fahari 7.5 34.3 1.0 14.3 Commercial released varieties<br />
Mean 0.87 12.73 0.15<br />
SE 0.41 2.98 -<br />
Range: 0-16 0-74 0-3<br />
Table 2: Yield (kg/ha) of cowpea entries at Alectra vogelii hot spot at three locations, 2009<br />
Cowpea Entries Ismani Bihawana Hombolo mean yield Comments<br />
FC<br />
across sites<br />
IT99K-7-21-2-2 372.8 527.1 568.7 489.6 Farmers selected for<br />
earliness<br />
IT99K-573-2-1 309.4 927.1 970.4 735.6 Farmers selected for yield<br />
IT97K-499-8 410.2 643.7 753.9 602.6<br />
IT97K-818-35 432.3 525.0 608.2 521.8<br />
IT97K-819-118 590.8 677.1 674.0 647.3<br />
IT97K-499-38 362.5 409.6 604.2 458.8<br />
IT89KD-288 413.9 639.6 773.5 609.0<br />
IT00K-1207 262.9 545.8 515.2 441.3<br />
IT96D-733 404.6 302.1 514.3 407.0<br />
B301 164.8 952.1 789.2 635.4<br />
TZA 263 265.6 525.0 386.6 392.4 Large grain but severely<br />
infected by Bacterial blight<br />
disease.<br />
Vuli 2 212.7 354.1 867.3 478.0<br />
Fahari 209.8 554.2 745.6 503.2<br />
Mean 331.70 583.24 674.68<br />
SE 29.44 35.15 27.86<br />
Range: 92.5- 108.3- 236.6-<br />
791.7 1542.0 1198.0<br />
1.3.3a: On-farm evaluation of cowpea lines for Alectra resistance and yield:<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Introduction: Most farmers are growing long duration trailing type of cowpea, and mostly around their<br />
homestead. They are mainly for getting leaves and few pods. The grain yields are usually very low due<br />
to low yield potentials of the cultivars and farmers do not spray against insect pests at flowering and<br />
podding, hence, most of the pods are lost. In addition, the local varieties are very susceptible to Alectra<br />
vogelii. The introduction of early, Alectra resistant and high yielding cowpea varieties will improve the<br />
nutritional status and increase income to the households by selling extra produced grain. There is high<br />
potential market for cowpea and for improving household nutritional status.<br />
Material and Methods: Due to shortage of cowpea seeds, five villages were selected for evaluation of<br />
five best selected cowpea lines from last season for Alectra resistance, adaptation and yield.<br />
Commercially released varieties Vuli-2 and Fahari were added and their village local checks. The<br />
villages involved were Mangalali and Mkungugu (Iringa region), Kikombo in Dodoma and other two<br />
villages were Msungua and Ikhanoda in Singida rural district.<br />
The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot,<br />
which were separated by one meter. Farmers wanted to have larger plots, but we were limited with<br />
amount of seed. With their chairman and secretary of the group and with assistance from extension<br />
officers farmers were able to collect data and evaluate the materials according to their criterion<br />
Results and discussion: As discussed under section 3.1 rains distribution was a major problem. It was<br />
late and erratic; hence there was very poor crop establishment such that many of farmers just managed<br />
to get few cowpea pods especially in Dodoma and Iringa sites. The other problem observed in Singida<br />
was for unforeseen outbreak of cut worms, which occurred at germination of the crop. Some farmers<br />
had to replant more than two times until they ran out of seed. In Singida rainfall started on time and it<br />
was relatively evenly distributed as compared to Dodoma and Iringa rural districts. The Alectra<br />
infestation was only reported on commercial and local cultivars, the other lines were observed free from<br />
the weed pest<br />
The yields recorded on few farmers who managed to get a crop are as presented in Table 3.. The<br />
average highest cowpea yield obtained was from Singida rural followed by Iringa and last Dodoma rural<br />
district.<br />
Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania 2009<br />
Cowpea line Farmer groups ir<br />
Mkungugu Kikombo Msungua<br />
Iringa Dodoma Singida<br />
IT99K-7-21-2-2-1 600 222 600<br />
IT99K 573-2-1 720 440 600<br />
IT97K 818-35 200 389 800<br />
IT89KD 288 400 333 200<br />
TZA 263 280 167 1200<br />
VULI-2 560 - 600<br />
FAHARI 800 - 760<br />
Village Local 200 - 530<br />
Mean 470.0 310.2 661.3<br />
1.3.3b: Farmer Participatory Cowpea Lines Evaluation<br />
Introductions: Cowpea (Vigna unguiculata (L) Walp), is one of the African indigenous most important<br />
leguminous crops. It is mostly grown in tropical countries for various uses and is a cheap source of<br />
vegetable protein. The crop is well adapted to relatively dry and stressful growing conditions of the<br />
marginal land, but produces excellent nutritive value (Singh et al., 1997). It is a drought tolerant crop<br />
and recently, there has been an increasing demand of cowpeas in the human diet, as well as for income<br />
generation. It thus provides solution for periodic hunger in semi-arid region of Tanzania<br />
6<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
This is the second season that the on farm evaluation of most promising lines of cowpea has been<br />
conducted in five villages namely; Msungua and Ikhanoda (Singida region), Kikombo (Dodoma region)<br />
and Mangalali and Mkungugu (Iringa region). Building from the 2008 evaluation, objectives of the 2009<br />
evaluation were as follow:<br />
~ To revisit the 2008 results<br />
~ To revisit evaluation criteria used in 2008<br />
~ To evaluate cowpea lines planted in 2009<br />
~ To make informed conclusion to guide for the subsequent on farm evaluation<br />
This brief presents the outcome evaluation of farmers’ trials in five villages in the above mentioned<br />
districts<br />
Method: The evaluation mission comprised Dr A.M. Mbwaga (Pathologist), Dr J.K. Mligo (Legume<br />
breeder) and Dr J.P. Hella (Agricultural Economist). As in 2008, the team participated in the on-farm<br />
evaluation in all villages for a period between 17 th and 24 th May 2009. In 2008 evaluation involved<br />
traversing with farmers in all five villages in each plot planted with a known variety of cowpea. Farmers<br />
were then requested to list, based on their own experience, the criteria which govern them to value and<br />
prefer particular varieties than another variety. Although main criteria varied across villages, but mostly<br />
hanged on high yield, early maturity, resistant to pests including Alectra, big sized seed and white to<br />
cream colour.<br />
Observations<br />
Criteria for selecting cowpea cultivar by villages in 2008<br />
Table 4 present the criterion used by farmers to rank different cowpea lines. The results were almost the<br />
similar in all villages. Good and more preferred lines are those associated with high yielding, early<br />
maturity, pest/disease (including Alectra) and drought resistant, large and white/cream seed colour.<br />
Table 4: Criteria for selecting different cowpea lines by village<br />
Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />
Early maturity High yielding High yielding High yielding High yielding<br />
Resistance to Alectra, Early maturity Drought Large seeds Large seeds<br />
diseases/insects/pests<br />
resistance<br />
High yielding varieties<br />
with many leaves<br />
Cream color Early maturing Early maturity Early maturing<br />
Brown color Alectra Resistant to Tolerant to Alectra<br />
resistant Alectra Alectra tolerance<br />
Big size- seeds Big size seed Cream Seed Reddish seed Drought<br />
color<br />
colour<br />
resistance<br />
Large seeds Plenty green<br />
leaves<br />
Cowpea lines preferred by farmers in target villages 2008<br />
Based on criteria highlighted in Table 4 above, in May 2008, farmers evaluated 14 lines including the<br />
commercial released varieties i.e. Vuli-2 and Fahari and locally grown cultivars named by the respective<br />
village name. List of cowpea lines evaluated in 2008 is presented in Table 5 below:<br />
Table 5: List of cowpeas line planted and evaluated in 2008 season<br />
No Lines/cultivar<br />
1 IT 99K-7-21-2-2-1<br />
2 IT 99K-573-1-1<br />
3 IT 97K-499-8<br />
4 IT 97K-818-35<br />
5 IT 97K-819-118<br />
6 IT 97K-499-38<br />
7<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
7 IT 89KD-288<br />
8 IT 00K-1207<br />
9 IT96D-733<br />
10 B 301<br />
11 TZA 263<br />
12 Vuli-2<br />
13 Fahari<br />
14 Farmer’s variety<br />
(Msungua/Ikhanoda/Kikombo/Makungugu/Mangalali)<br />
Table 6: Cowpea lines selected by each participating farmer Group in 2008 based on criteria in<br />
Table 4<br />
# Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />
1 IT99K-7-21-2-2 TZ A263 IT 00K-1207 TZA 263 IT 99K-7-21-2-2<br />
2 IT97K-499-8 IT99K-7-21-2-2 IT97K-818-35 IT 99K-573-1-1 IT 97K-499-8<br />
3 IT99K-573-1-1 IT97K-818-35 IT97K-499-38 IT 97K-499-8 IT 97K-819-118<br />
4 IT00K-1207 IT97K-499-38 IT96D-733 IT 89KD-288 TZA 263<br />
5 IT97K-818-35 IT99K-573-1-1 B301, IT 97K-499-38 IT 99K-573-1-1<br />
6 IT97K-499-38 IT97K-499-8 T99K-573-1-1, IT 97K-818-35 IT 97K-499-38<br />
Ranking for 2009<br />
At the beginning of the season, five out of 14 lines planted in 2008 were selected for planting at<br />
farmers’ plots. The lines selected for the current year was based in farmers’ own expression as indicated<br />
in Table 6 above. Lines presented for further evaluation and reasons advanced by farmers are as<br />
presented in table 7 below<br />
Table 7: Cowpea lines selected for on-farm experiment in 2009<br />
# Lines Varieties<br />
1 IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding,<br />
white cream coloured seed,<br />
2 IT 99K 573-1 Slightly late maturity, resistant to Alectra, average seed size, white<br />
cream seed colour, high yielding<br />
3 1T 97K 818-35 Late maturity, resistant to Alectra<br />
4 1T 89KD 288 Late maturity, high yielding, resistant to Alectra, white cream seed<br />
colour,<br />
5 TZA 263 Late maturity, resistant to Alectra, reddish brown colour, large seeded<br />
Msungua -Village, Singida<br />
In Msungua village, 16 farmers participated at the on-farm evaluation. Results are as presented in Table<br />
8 below. Line IT 99K 7-21-2-2-1 was the most preferred and IT89KD288 was the least preferred line.<br />
Table 8: Pair-wise ranking for Msungua village<br />
Lines 1 2 3 4 5 Result Rank<br />
IT 99K 7-21-2-2-1 1 1 1 1 4 1<br />
IT 99K 573-1-1 2 2 2 3 2<br />
1T 89KD 288 4 5 0 5<br />
1T 97K 818-35 5 1 4<br />
TZA 263 2 3<br />
8<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Ikhanoda-Village, Singida<br />
At Ikhanoda village, 12 farmers participated at the on-farm evaluation and results are as presented in<br />
Table 9 below. Line TZA 263 was the most preferred while IT 89KD 288 was the least preferred line.<br />
Table 9: Pair-wise ranking for Ikhanoda village<br />
Lines 1 2 3 4 5 Result Rank<br />
IT 99K 7-21-2-2 1 1 1 5 3 2<br />
IT 99K 573-1-1 2 2 5 2 3<br />
1T 97K 818-35 4 5 1 4<br />
1T 89KD 288 5 0 5<br />
TZA 263 4 1<br />
Kikombo village, Dodoma<br />
In Kikombo village, 6 farmers participated at the on-farm evaluation and results are as presented in<br />
Table 10 below. Line TZA 263 was the most preferred but IT 89KD 288 was the least preferred line.<br />
Table 10: Pair-wise ranking for Kikombo village<br />
Lines 1 2 3 4 5 Result Rank<br />
IT 99K 7-21-2-2-1 3 2<br />
IT 99K 573-1 1 2 3<br />
1T 97K 818-35 1 2 1 4<br />
1T 89KD 288 1 2 3 0 5<br />
TZA 263 5 5 5 5 4 1<br />
Mkungugu –Ismani, Iringa<br />
In Mkungugu village, 10 farmers belonging to Ari Mpya farmer research group participated at the onfarm<br />
evaluation. Results are as presented in Table 11 below. Line TZA 263 was the most preferred and<br />
IT 97K 818-35 was the least preferred line.<br />
Table 11: Pair-wise ranking for Mkungugu village<br />
Lines 1 2 3 4 5 Result Rank<br />
IT 99K 7-21-2-2-1 1 1 1 5 3 2<br />
IT 99K 573-1 2 2 5 2 3<br />
1T 97K 818-35 4 5 0 5<br />
1T 89KD 288 5 1 4<br />
TZA 263<br />
Mangalali - Iringa<br />
5 1<br />
For unforeseen reasons it was not possible to evaluate the materials on day the team visited Mangalali<br />
village. When the farmers from Managalali visited Ismani research station, they were given opportunity<br />
to evaluate replicated trial planted with 14 lines including those planted at their own plots at Mangalali.<br />
The results are as presented in Table 12 below, where TZA 263 was ranked the best line followed by<br />
IT99K-7-21-2-2.<br />
Table 12: Ranking of the cowpea lines by Mangalali farmers at Ismani research Station 2009<br />
No Cowpea line Number of Number of Ranking of the Comment<br />
participants farmers selected cowpea line<br />
1 TZA-263 26 24 1 Each farmer<br />
2 IT99K-7-21-2-2-1 20 2<br />
was given a<br />
3 B 301 9 3<br />
chance of<br />
4 ITOOK-1207 8 4<br />
selecting only<br />
5<br />
6<br />
IT96D-733<br />
IT89KD-288<br />
8<br />
6<br />
4<br />
5<br />
best three<br />
cowpea lines<br />
7 IT99K-573-1 3 6<br />
9<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Conclusion<br />
The main objective of the field visitation was to evaluate farmer managed experiments in all study<br />
villages. Based on the approach and criteria agreed by the farmers, final rank on 5 lines introduced to<br />
them is presented in Table 10. The most preferred line is TZA, 263, followed by IT 99 K-7-21-2-2-1<br />
and IT 99K-573-1-1. Position four and five were contested between line IT 89KD-288 and IT 97K 818<br />
which were scored by two villages in the same positions. After revisiting some inherent characteristics<br />
of the lines as scored by same farmers in 2008, line IT 89KD-288 was relatively batter on same traits<br />
such as early maturity and leave sizes which are lacking in IT 97K-818. Thus IT 89KD-288 was ranked<br />
4 th while IT 97K-818 was ranked 5 th . as indicated in Table 10<br />
Table 10: Farmer preference with respect to 5 introduced cowpea LINES<br />
Villages Rank in order of preference<br />
1 st 2 nd 3 rd 4 th 5 th<br />
Msungua IT 99K 7-21-2-2-1 IT 99K 573-1-1 TZA 263 1T 97K 818-35 IT 89KD 288<br />
Ikanoda TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />
Kikombo TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />
Mkungugu TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 89KD 288 IT 97K 818-35<br />
Mangalali TZA 263 IT99K-7-21-2-2-1 1T89KD-288 IT99K-573-1 1T 97K 818-35<br />
Rank (1) TZA 263<br />
(2)<br />
IT 99K 7-21-2-2-1<br />
(3)<br />
IT 99K 573-1-1<br />
(4) IT 89KD 288<br />
(5) IT 97K 818-35<br />
Based on these findings, lines TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 are to be increased for<br />
distribution to many farmers for more evaluation. For these lines, initial process to establish descriptors<br />
for these lines for possible inclusion in the National Performance Trials (NPT) towards variety release<br />
should be started.<br />
1.3.4 Targeted crosses<br />
Tanzania cowpea crossing program<br />
In 2007 report 130 cowpea accessions were screened for Alectra resistance. 10 accessions could not<br />
support emergence of Alectra. The identified accessions that could not support emergence of Alectra<br />
plants were classified as resistant to Alectra. These were crossed to the desirable parents (the released<br />
varieties which are: Fahari, Tumaini, Vuli-1 and Vuli-2) with the aim of transferring the Alectra<br />
resistance into these released varieties. Since resistance to Alectra is conditioned by a single gene, a<br />
backcross method was used to transfer of resistance. At present the screening (September, 2009) is at<br />
backcross three plants. The resistant plants will be identified while they are still flowering, thus the<br />
resistant backcross three plants will be backcrossed to the desirable parents to obtain backcross four<br />
seed which will be planted and selfed then followed by screening for resistance to Alectra. This process<br />
will continue up to backcross six at which it is expected a single chromosome segment carrying the<br />
desired gene will have been incorporated into the desired parents. Planting of backcross four seed will<br />
be conducted in early November 2009. The selfed backcross four seed will be planted in late February<br />
2010 for screening again for resistance to Alectra and resistant plants backcrossed to the desired parent<br />
to produce backcross five seed. Backcross five seed will be planted for selfing in early May 2010 and<br />
the selfed backcross five seed will be planted for screening for Alectra resistance in early august 2010.<br />
Resistant plants will be backcrossed to desirable parent in October 2010 to produce backcross six seed.<br />
Backcross six seed will be selfed ready for evaluation for Alectra resistance and yield on Alectra<br />
infested fields in season 20010/011 with the expectation that the project will be extended.<br />
10<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Table 14: Backcross three crosses in the screen house at ARI-Ilonga by September 2009<br />
Cross number Pedigree<br />
17 (B301 x Vuli-1) x Vuli-1<br />
30 (IT 97K-499-8 x Vuli-2) x Vuli-2<br />
38 (Tumaini x IT96D-733) x Tumaini<br />
48 (ITK-499-8 x Tumaini) x Tumaini<br />
Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />
Activity 1.4.1: Across site evaluation (PL to visit programme in Malawi)<br />
A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra project activities<br />
The team was composite of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />
leader and Charlie Riches, CoP liaison officer and met by the Malawian project team that was<br />
composite of Dr Vernon Kabambe country project leader, Mosses Mamiliro (breeder) ,and James<br />
Bokasi (breeder)<br />
The evaluation tour for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro) from 27 th<br />
to 30 th April, 2009. The tour involved visiting seed multiplication, on-farm, on-station, and crossing<br />
programme. The team comprised of the following:<br />
Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />
Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />
Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />
James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />
Boukar Ousmane (Breeder,) IITA Nigeria<br />
Activity 1.4.2: Student projects<br />
For Tanzania an MSC student working on a project “Assessment of cowpea marketing efficiency; A<br />
case of selected regions of Tanzania” was completed and the student graduated November 2008.<br />
In Malawi an MSc student working on a project “Farmer Participatory Selection of Alectra Resistant<br />
Cowpea Varieties in Selected Districts of Malawi”. The thesis has been submitted to examiners for<br />
review. In addition in Malawi an BSc student is undertaking a study on “Cowpea variety x time of<br />
planting x density”<br />
Activity 1.4.3: Paricipation in CCRP "community of practie<br />
Three researchers, one extensionist and one farmer from the project attended CoP2 in Maputo<br />
Mozambique in October 2008. In CoP3 in Bagamoyo Tanzania 4 researchers and 4farmers from project<br />
sites attended<br />
Activity 1.4.4: End of project stakeholder’s workshop (location to be determined later)<br />
Output 1.5: Project monitored and evaluated<br />
Activity 1.5.1. Establish project level M & E criteria<br />
(done during partners meeting in Mbeya at the inception of the project)<br />
Activity1.5.2. Review progress against M & E criteria each year<br />
Introduction: A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra<br />
project activities.<br />
The team was composed of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />
leader and Charlie Riches( CoP- liaison officer) and met by the Malawian project team that was<br />
composed of Dr Vernon Kabambe country project leader, Mosses Mamiliro( breeder), and James<br />
Bokosi (breeder).<br />
The objective of the visit were<br />
To evaluate on going on-farm cowpea activities, on station project activities and crossing work in screen<br />
house<br />
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On –farm activities: On farm activity included evaluation of 7 cowpea cultivars of which 5 were<br />
experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-2-2-1, IT 97K 825-15, Bunda 1,<br />
IT98K503-1) one commercially released variety (IT 82E-16) and a farmer’s variety at each project site.<br />
The project sites included Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu<br />
district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA.<br />
Lilongwe district<br />
Ngwangwa Extension Planning Area (EPA)<br />
a. Mazila village: A farmer research group in Ngwangwa was composited of 30 members but due to<br />
shortage of cowpea seed only four farmers planted the 7 cultivars. The general performance of the<br />
cultivars was very poor which resulted in poor pod setting. The possible reasons are: the soil fertility<br />
looked very poor, also it appeared to have had high insect incidences.<br />
The Alectra infestation was very high especially on the released variety (IT 82E-16) and Bunda 1. Less<br />
infestation was observed on IT97K-825-15, IT99K-494-6 and IT99K-7-21-2-2-1.<br />
b. Mpondela: Here the group was able to visit field of one farmer. There was good performance of all<br />
the entries planted and there was no Alectra observed in all of the seven entries.<br />
Kasungu District<br />
Lisasi EPA<br />
Sikondeyani village: Five farmers planted the trial. The trial performance was very good compared to<br />
those of Ngwangwa EPA. This may be due to good soils and relatively good rain Although nearby fields<br />
planted with cowpea had very high Alectra infestation but the infestation levels in the trials was<br />
relatively low and late emergency for this reason the grain yield was not much affected. Farmers<br />
selected IT82E-16 and Bunda 1 as the best varieties in terms of yield and earliness.<br />
Zomba district<br />
Mpokwa EPA<br />
Khuvinda village: There was good performance of the cowpea lines with very low Alectra infestation.<br />
Out of the seven entries, two entries (IT82E-16 and Bunda 1) were selected by farmers to be the best<br />
due to their earliness and good pod setting. Although the released variety IT82E-16 is a short duration<br />
variety, farmers were not aware of such type of the variety. This emphasizes the need to carry out more<br />
demonstrations to create awareness to more farmers.<br />
Balaka district:<br />
Rivirivi EPA: This is the only area where farmers were not organized in a formal farmer research group.<br />
The demonstrations were very good and planted along the road side for everybody to see. Alectra<br />
infestation was very low. As in other villages, here farmers also selected the early maturing varieties as<br />
the best cultivars.<br />
Bunda College: Activities here included, seed multiplication of experimental cowpea lines which have<br />
shown Alectra resistance. These included TZA 263 (from Tanzania) and IT 81D-994. Seed increase also<br />
included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and IT82E-16. The<br />
performances of the materials were good and were at flowering stage. The materials for crossing were<br />
planted in screen house and were at vegetative stage. Some of them had supported Alectra emergence<br />
already, which were recommended to be removed out of the experiment because they had shown<br />
susceptibility to Alectra.<br />
Lessons learnt:<br />
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Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least preferred by<br />
the farmers. At all sites farmer’s variety was the latest, and it had not even flowered when all the other<br />
entries were maturing.<br />
It was noted that only early maturing varieties were most preferred, forgetting the advantage of late<br />
maturing varieties for having other traits such as prolonged availability of leaves as relish. This suggests<br />
that there is a need to evaluate the materials according to maturity groups.<br />
Cowpea and pigeon pea as the main source of vegetable protein in the project target sites; it was<br />
observed that early maturity of the cowpea bridge the hunger period of February/March, while waiting<br />
for the pigeon pea to mature late in the season.<br />
Wrap up:<br />
- Released varieties showed better yields than test lines<br />
- Early maturing varieties save as source of food in hunger periods<br />
- Seed system for cowpea is very poor<br />
- More cowpea seed should be sent to Balaka and Kasungu districts as there is high demand for seed<br />
of the improved varieties.<br />
- Farmers in Rivirivi recommended forming farmer research groups for better access by researchers<br />
and extension staff as compared to individual isolated farmers<br />
TANZANIA<br />
27 th -30 th April, 2009<br />
Introduction: The monitoring and evaluation tour for Tanzania was done in three regions (Iringa,<br />
Dodoma, and Morogoro) from 27 th to 30 th April, 2009. The tour involved visiting seed multiplication,<br />
on-farm, on-station, and crossing activities. The objective was to monitor progress in implementing<br />
agreed strategies and action plans for the project. This report gives information on sites/farmers/stations<br />
visited, varieties evaluated, team observations and way forward.<br />
The monitoring and evaluation team comprised the following:<br />
Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />
Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />
Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />
James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />
Boukar Ousmane (Breeder,) IITA Nigeria<br />
On-farm visits: A number of farmers groups were visited in the two regions (Iringa, and Dodoma). In<br />
general farmers were required to plant five IITA lines and two locally released varieties (Fahari and<br />
Vuli-2). The two released varieties showed to be more adapted and performed consistently well across<br />
locations despite the prevalence of drought in many locations. The major problem is that they are both<br />
susceptible to Alectra. In a participatory variety trial conducted at Mpunguzi, Dodoma region, farmers<br />
selected IT 00K-126-3 as the best entry despite being susceptible to Alectra. Many of the farmers<br />
visited showed dedication to running the on-farm trials.<br />
On-station visits: The first on-station trial visited was at Ismani. The trial had 14 entries replicated four<br />
times. Each plot had five rows of four meter length. Although drought had an impact the performance<br />
of the entries was far better than the farmers’ fields. Alectra pressure was not as high as the previous<br />
year. This could have been due to the prolonged dry spell during the growing season. Vuli-2 and Fahari<br />
performed very well despite sustaining some Alectra plants. Except for the two check entries the rest<br />
had no Alectra emergence. The second trial was at Bihawana (Bihawana farmers training centre). The<br />
entries were similar to those planted at Ismani but four extra entries. Rainfall distribution was not good.<br />
The site received a mere 250 mm (normal 330 -488 mm) of rainfall between February (151.3mm) and<br />
March (98.8mm) and poorly distributed. This site had the heaviest infestation of Alectra and is good for<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
screening. High insect population was observed at this site as compared to the other sites. Vuli-2<br />
looked good but not outstanding. Entry IT 573-1 doing well and B301 though small seeded. <strong>Crop</strong><br />
management was very good despite drought. IT99K 573-1 and B301had no Alectra in all four<br />
replicates. Two entries, IT99K-7-21-2-2-1 and TZA 263 had only one Alectra plant in one replication.<br />
The third on-station trial was at Hombolo. The entries were same as those planted at Ismani and<br />
Bihawana. The planting date was 16 th January, 2009 and germination occurred on 4 th February, 2009<br />
because of lack of rain after planting. However the Alectra pressure was not high. Field management<br />
was good and the entries performed well.<br />
Seed production: A major objective of this activity was to teach farmers on how to produce quality<br />
seed. The first seed multiplication activity visited in Iringa was at a community plot known as. Three<br />
varieties were planted (Fahari, Vuli-2, and local cultivar). The varieties were planted on 25 th February,<br />
2009 and entries were just starting to flower. The isolation distance was 2.5 meters and crop looked<br />
very clean. Dimethoate was used to control aphids. The soil was quite sandy. Farmers claim late<br />
planting was due to late land preparation because other farmers contributed late for hiring oxen used for<br />
land preparation. There was poor effort in labelling entries.<br />
The second visit was at Mpunguzi, Dodoma region. Two farmers planted one variety (Fahari) on 2 nd<br />
February, 2009. One farmer had a poor field but the other one managed the crop well (one acre) and will<br />
harvest a good yield despite the drought.<br />
Cowpea crossing programme at ARI Ilonga<br />
The visit to see crossing programme was only to Ilonga research institute where the crossing for cowpea<br />
varieties to improve resistance to Alectra is being conducted. This station is in Kilosa district. The<br />
research mandate includes other crops such as maize, sorghum, soybean, sunflower, cotton, pearl millet<br />
besides cowpea. The crossings were done in a screen house and involved commercially released<br />
varieties as recurrent parents in a backcrossing program such as Tumani, Fahari, ,Vuli-1 and Vuli-2.<br />
Aphid infestation is a major problem in the screen house. Backcross 3 seed had been harvested ready for<br />
planting to make backcross 4.<br />
WAY FORWARD/RECOMMENDATIONS:<br />
Drought (rainfall, poor distribution and low amounts) has been an overriding problem this season. We<br />
commend the collaboration the project is undertaking along with the tropical legumes II project of IITA.<br />
The project should therefore incorporate both traits (Alectra resistance and Drought tolerance). An<br />
analysis of medium term climatic data (20-25 years) would be important to determine the frequency of<br />
rainfall events (e.g. start dates, dry spells and rainfall amounts). This can help to determine the<br />
important drought traits in plant (e.g. earliness or resilience). The good production practices should be<br />
incorporated in the screening of the varieties against Alectra. Hence, there is need to expose the groups<br />
involved in the project to all these production practices. There is a need to evaluate the materials<br />
according to maturity group early, medium and late because farmers’ selections are dictated and<br />
influenced by time to maturity. The experiences observed from this project and TLII project indicate<br />
that the overriding preference trait is seed size; large seeded types are more liked than small seeded<br />
types. Thus this should be taken care in our project. The backcrossing programme at Ilonga will<br />
continue to backcross 6 by October 2010, while at Bunda will be at back cross 4. Request for extension<br />
Objective 2: Promotion of high yielding A. vogelii resistant cowpea cultivars<br />
Output 2.1 Farmer and extension awareness of Alectra vogelii and use of resistant cultivars<br />
Activity 2.1.1: Develop leaflets on crop management and ultilization of cowpea, pre-test and print<br />
A draft leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These<br />
were produced in Kiswahili for better understanding by farmers. It emphasized on the different ways of<br />
cowpea utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be<br />
converted into other value-added products such as dhal, stew and snack products like “bagia”. This<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
leaflet has been printed out for further dissemination of the technologies. The leaflet on crop<br />
management is to be produced next cropping season<br />
Activity 2.1.2a: Farmer exchange visits and field days across project sites<br />
Farmer exchange visit involved twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu<br />
(Fahari Group) farmer <strong>Research</strong> Group and also at Ismani On-station trials in Iringa. These were also<br />
accompanied by their extension officers from the respective villages and from the district. The objective<br />
of the farmer exchange visit was farmers from Mangalali to get experiences from other farmers on<br />
principals of cowpea production, processing and utilization as well as how to manage farmer group and<br />
then compare with the way they do in their own group. Also jointly went to research station to learn new<br />
materials and management at the research station.<br />
15<br />
2009<br />
Fig.3. Farmers discuss with their host<br />
about seed production at research plot<br />
of Mangalali <strong>Research</strong> Group, 2009<br />
At Ismani research station, farmers from both Mkungugu and Mangalali participated at evaluation of the<br />
14 cowpea screening materials planted. The outcome from their selection of the lines according to their<br />
criterion is shown in the Table-15Below.<br />
Table 15: Ranking of the cowpea lines planted at Ismani <strong>Research</strong> station 2009<br />
No Cowpea line Number of Number of Ranking of Comment<br />
participants farmers selected the cowpea<br />
line<br />
1 TZA-263 26 24 1 Each farmer was<br />
2 IT99K-7-21-2-<br />
20 2<br />
given a chance<br />
2-1<br />
of selecting only<br />
3 B 301 9 3<br />
best three<br />
4 ITOOK-1207 8 4<br />
cowpea lines<br />
5 IT96D-733 8 4<br />
6 IT89KD-288 6 5<br />
7 IT99K-573-1 3 6<br />
Table 16: Ranking of cowpea by Ari Mpya Farmer <strong>Research</strong> Group Mkungugu 2009<br />
No Cowpea line Number of Number of Ranking of Comment<br />
participants farmers who the cowpea<br />
selected line<br />
1 TZA-263 23 21 1 Each farmer was<br />
2 IT99K-7-21-2-<br />
17 2 given a chance<br />
2-1<br />
of selecting only<br />
3 IT89KD-288 17 2 best three<br />
4 B 301 9 3 cowpea lines<br />
5 IT99K-573-1 5 4<br />
At both sites farmers chose two best cowpea lines, which are TZA 263 for large grain size and IT99K-7-<br />
21-2-2-1 for its earliness.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Activity 2.1.2b Farmers participate at National Agricultural Show in Dodoma 2009:<br />
Two farmers from each of the farmer groups Msungua (Mshikamano FRG) and Ikhanoda (Mfwenji<br />
FRG) organized by the District Council represented their farmer groups at National Agricultural show,<br />
which took place in Dodoma from 1-8 August 2009. The farmers displayed their cowpea varieties and<br />
products processed from cowpea such as bans. This was a good motivation for the farmers.<br />
Output 2.2: Nutrition status of farmers improved<br />
Activity 2.2.1 Inventory of recipes of cowpea products<br />
This activity was undertaken in 2007/08 and completed and made a basis for the following activities<br />
Activity 2.2.2a: Develop/modify / demonstrate cowpea products:<br />
Introduction: Cowpea (Vigina unguiculata) is an important food legume and an integral part of<br />
traditional cropping systems in the semi arid regions of the tropics (Ryan et al, 1984). Although<br />
indigenous to Southern Africa, cowpeas has spread world wide and is extensively cultivated in semiarid<br />
and consumed in many regions of Africa including Tanzania.<br />
Farmers in the project sites produce many varieties of legumes particularly cowpeas, which are<br />
important for food and cash income for household. Thus make leguminous crops rank second after<br />
cereals such as maize and sorghum. Cowpea is a preferred staple food and its desirability reflects the<br />
fact that the leaves, immature pods, fresh seeds, green pods and dry grains can be eaten or marketed.<br />
The dry grains are commonly eaten whole or can be milled and consumed in numerous traditional main<br />
snack foods (E. Sarakikya, 1996). Cowpea grains as well as the vegetable parts, make major nutritional<br />
contributions to diets. Nutritionally, cowpea plays a critical subsistence role in the diets of many<br />
households by providing nutrients that are deficient in cereals.<br />
In order to add value, cowpea grains need to be processed. The processing of cowpea grains improves<br />
palatability, digestability, acceptability, reduced cooking time and increases market value of products<br />
made from dhal. This section therefore, sought to improve cowpeas utilization through nutrients<br />
addition to different products.<br />
The main objective of this study was to promote the utilization of cowpea in Tanzania.<br />
• Train farmers on the processing of cowpea grains into dhal<br />
• Preparation of cowpea dishes (snack bites from cowpea flour “bagia’)<br />
• Perform sensory evaluation tests.<br />
Expected outputs<br />
• Smallholder farmers’ capacity and capability on ways of using processed cowpea products built.<br />
• Smallholder farmers, skills and knowledge in value addition on cowpea products enhanced and<br />
strengthened<br />
• Cowpea products utilization promoted<br />
• Nutrition status for the families in project sites improved<br />
Methedology<br />
Study area<br />
The training was conducted in Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />
Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The sites were<br />
purposively selected as a result of their involvement in a project on “Improvement of cowpea<br />
productivity on Alectra Vogelii infested land in Tanzania.<br />
Materials used<br />
Materials used to perform and conduct this study include, flip charts, board, cowpea grains, cowpea<br />
flour, cowpea dhal, ingredients such as onions, garlic, eggs, chilies, cooking oil and salt.<br />
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Approach<br />
A participatory research approach of working with organized village farmer groups was adopted. These<br />
included participatory research models such as demonstrations, displays and training through hands-on<br />
experience. Appreciative problem solving methods were used to encourage farmers learn, explore and<br />
practice new skills and knowledge.<br />
Sample size<br />
The number of farmers in each group varied between 15-35 members. Level of women representation<br />
was 58 percent of the total number. The study participants were mainly smallholder farmers drawn from<br />
eight villages in three regions. These farmers provided the information on producers and consumers.<br />
These farmers mostly grew cowpeas for subsistence and they were the one who tackled the issue of<br />
consumer preference. Other participants included government officials, district and village extension<br />
officers.<br />
Training of farmers and local processors<br />
A one day workshop was conducted in each project area and farmers were trained on improved cowpea<br />
technologies. The trainings were based on demonstrations, hands-on trials and printed materials<br />
(bronchures for cowpea recipes). Farmers participated in the validation of five recipes for cowpea value<br />
addition. Sensory tests were carried out, where by farmers were required to rank and find out the most<br />
preferred type of”bagia”. The five types of bagia were made using different recipes (see Table 1). The<br />
recipes were translated into national language for better understanding.<br />
Table 18: Cowpea snack product recipes<br />
Bagia type Materials/ingredients<br />
1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />
2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />
3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and<br />
water<br />
4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and<br />
water<br />
5. bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs,<br />
yeast and water<br />
Common method used<br />
• Mix all the ingredients together<br />
• Add water and mix well<br />
• Make the dough by needing (allow the dough to rise by leaving it for 10-15 minutes)<br />
• Heat cooking oil until really hot.<br />
• Use spoon or hand to make the size of bagia you need and put the dough into the oil<br />
• Stir and cook until brown<br />
• Remove from oil and drain<br />
• Cool and pack for marketing or save for consumption.<br />
Farmers were equipped with skills and knowledge on quality control with emphasis on the storage<br />
techniques. Also they were informed about product grading/sorting, market requirements of quality,<br />
quantity and sustainability. The training also emphasized on the methods of de-hulling whole cowpea<br />
grain for dhal production that can be converted into other value added and snack products, such as<br />
“bagia”. The value added products are considered nutritious and sources of income generation. It was<br />
however, observed that the prevailing low production levels, lack of adequate and appropriate storage<br />
facilities constrained the sustainability of the niche markets.<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Dissemination of information<br />
A Leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These were<br />
produced in Swahili for better understanding of farmers. It emphasized on the different ways of cowpea<br />
utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be converted<br />
into other value-added products such as dhal, stew and snack products like “bagia”. Value-added<br />
products are considered nutritious and sources of income generation. It was also observed that storage of<br />
cowpea grain was a big problem but dehulling the grains could improve storage up to many years. Also<br />
this reduces the labour involved in preparation by women, wherever they come to prepare Bagia.<br />
It is envisaged the disseminated materials can always be referred to by farmers/ processors in case of<br />
problem shooting long after the project expires.<br />
Results and Discussions<br />
Cowpea preference<br />
The study found that, the current type of cowpea grown in the project sites take more than two hours to<br />
cook. The smallholder farmers/consumers prefer cowpeas that cook in less than two hours. Hence<br />
training of cowpea grain processing into dhal as on alternative of reducing cooking time was important.<br />
Sensory evaluation<br />
“Bagia” samples were prepared using the above recipes. Each bagia type reflected to the type of recipe.<br />
At the time of testing the samples were coded 1,2,3,4, and 5 for bagia types made from recipes 1 to 5<br />
respectively. A panel of respective farmers, extension officers and village government leaders<br />
participated in the trial. They were given the five samples of bagia not simultaneously, one at a time and<br />
they were asked to assign preference score on products attributes including taste, texture and<br />
palatability. A pair-wise ranking method (Table19) was used to find out the most preferred bagia type.<br />
Panelists were asked to taste and rank the overall acceptability of the product in relation to that of the<br />
other four. Every sample was compared to each other by allowing every panelist to give opinion or rise<br />
up hands for most preferred sample. The scores of the quality attributes were done using the following<br />
rating scale;<br />
1 = excellent<br />
2 = very good<br />
3 = good<br />
4 = average<br />
5 = poor<br />
The overall acceptability was ranked from 1-5 (Table 19)<br />
Table 19; Ranked cowpea “bagia”<br />
Bagia type Rank<br />
1 5<br />
2 3<br />
3 4<br />
4 2<br />
5 1<br />
Table 2 shows that, bagia type 5 was the most preferred by farmers, while bagia type 1 was the least.<br />
This indicates that, bagia enriched with nutrients (type 5) came out as the most preferred as compared to<br />
“bagia asilia”(type 1), which was not enriched with nutrients (See Table 19). It is evident that,<br />
palatability and taste to a large extent is associated with nutrients enrichment. According to the panelist<br />
views, it is believed that if a product is palatable and nutritionally enriched, it is most likely to fetch<br />
more market than the reverse. Therefore value added products, apart from being nutritious they are<br />
considered palatable and sources of income generation.<br />
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Table 20: Summary village score and rank data<br />
Bagia type Village scores and rank<br />
Singida region Dodoma region Iringa region<br />
Msungua Ikhanoda Nduu Kikombo Mpunguzi Mkungugu Ilambilole Mangalali<br />
S R S R S R S R S R S R S R S R S R<br />
1.Bagia asilia 0 4 0 5 0 5 4 1 0 3 0 5 0 5 0 5 0.5 3.5<br />
2. Bagi +<br />
viungo<br />
3.Bagia +<br />
viungo+hamira<br />
4. Bagia +<br />
viungo+<br />
mayaia<br />
19<br />
2009<br />
Aver<br />
age<br />
Av.<br />
rank<br />
2 2 2 3 4 1 3 2 3 1 2 3 2 3 4 2 2.8 2.1<br />
1 3 1 4 1 4 0 5 1 2 1 4 1 4 1 4 0.9 3.8<br />
4 1 4 1 2 3 1 4 3 1 3 2 3 2 2 3 2.8 2.1<br />
5.Bagia +<br />
viungo+ mayai<br />
+ hamira<br />
4 1 3 2 3 2 2 3 3 1 4 1 5 1 3 1 3.4 1.5<br />
NB: S= Score, R= Rank<br />
Storage and storage insect pests:<br />
Cowpea grain is very susceptible to storage pests especially to bruchids hence store poorly. This has<br />
been one of the constraints that farmers do not keep much of their cowpea grains more than 6 months.<br />
On the other hand dehuled cowpeas are not infested by storage pests and can bee stored for a long time<br />
so long they are kept in a dry place.<br />
CONCLUSIONS AND RECOMMENDATIONS<br />
Initially, cowpea production has mainly been for household use and limited sales to local/village<br />
markets. Thus by providing farmers with the technologies for use in the improvement of the processing<br />
of cowpea into dhal, this study has opened the community to new avenues for storing the cowpea<br />
products longer and obtaining income from the crop.<br />
By achieving the diversification of small-scale rural farmers’ incomes through adoption of appropriate<br />
post-harvest technologies and providing information on the value addition for cowpeas, the project has<br />
equipped farmers with means of enhancing their household incomes. This will be enhanced through<br />
better quality products which will ensure small-holder farmers’ ability to have market access for them.<br />
Additionally, through equipping farmers with knowledge and skills of improved handling, processing<br />
and utilization of cowpea products, the capacity of the rural communities in which the project has<br />
operated, to earn an income from similar processed products through value addition has been enhanced.<br />
Also the project has built the capacity of the rural small-scale cowpea farmers in working in groups and<br />
organizing themselves to produce necessary volumes and quality requirements cowpea grain which can<br />
meet market requirements. The project further enabled the farmers to change their perception of cowpea<br />
as a crop for household consumption and small scale production, to perceiving it as a possible income<br />
earner with potential to increase household incomes.<br />
However, this study highlighted the fact that, what is needed at times is change in attitude of farmers<br />
towards a crop in order to enable them increase its production and make an income from marketing its<br />
products.<br />
It is recommended that, farmer groups need to be facilitated and equipped with means to obtain storage<br />
facilities for large volumes of cowpea grains, especially since large quantities of grains are expected to<br />
be produced.<br />
Finally, it is recommended that, this study is replicated in other areas of the country and other crops<br />
characterized by low utilization base.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Way forward<br />
There a need to have a follow-up study to evaluate post-harvest technologies taught/taken to smallholder<br />
farmers in project sites. This will be a kind of monitoring and evaluation study, it may be done routinely<br />
to evaluate the rate of adoption of post-harvest technologies taught/taken to farmers.<br />
The study will include the following activities;<br />
• Post-harvest technologies taught<br />
• Cowpea handling, processing, storage utilization and marketing<br />
Indicators<br />
- Level and constraints limiting the use of technologies on cowpea handling, processing, storage,<br />
utilization and marketing<br />
- Processing methods<br />
- Storage techniques<br />
- Forms and rate of cowpea consumption at household and community levels<br />
- Cowpea preferences on for example product taste, texture, grain size, skin colour, type of<br />
products, etc<br />
- Cowpea marketing value chains<br />
- Types of value added cowpea products used and sold at the markets, at what type and level of<br />
markets, Access and market linkages established/existing<br />
- The general level, use and application of improved technologies.<br />
References<br />
Eva Pendeli Sarakikya, 1996, Tanzania Cook Book, Tanzania Publishing Limited,<br />
Dar es Salaam.<br />
J.K. Ryan, P.D. Bidinger, N. Prahlad Rao, p.Pushpamma, 1884, The Determinants of<br />
Indivicual Diets and Nutritional Status in six villages of South India, ICRISAT, India<br />
Activity2.2.2b: Analysis of nutrient content in cowpea grain and products<br />
Studies on micronutrients: Minerals of nutritional importance that were analyzed included iron, zinc<br />
and calcium; results indicate that, there was twice much calcium in leaves than in cowpea grains and<br />
almost 40 times as much iron. Since iron and zinc levels in cowpea leaves have shown to be<br />
significantly higher than the levels in cowpea grains, creating more awareness and encouraging the<br />
utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies from cheap sources<br />
especially from resource-poor families. The variety that was best with regards to all minerals was<br />
IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and fats content<br />
were IT97K499‐8 and IT99K‐7‐21‐2‐2‐1.<br />
Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />
seed identified:<br />
Activity 2.3.1: Identification of seed multplication mechanism<br />
- Available sources of seed or classes of seed are<br />
- Breeder seed<br />
- <strong>Foundation</strong> seed<br />
- Certified seed<br />
- Quality Declared Seed (QDS)<br />
From these classes farmer groups or communities can produce QDS from foundation seed or certified<br />
seed. The produced QDS can only be sold within the district. However, only trained Farmer<br />
Groups/Farmers can be contracted by Agricultural Seed Agency (ASA) or any other seed company to<br />
produce certified seed that can be sold beyond the production area.<br />
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Sub-Activity2.3.1.1: Train farmers to produce seed (QDS) for commercial use:<br />
Training of farmers on Seed Production (QDS) of Cowpea is very important because the crop saves<br />
many lives of people in drought prone areas of the semiarid areas where the crop is grown. The seed<br />
availability is a big issue because commercial companies are not interested in producing seed of low<br />
volume crops like sorghum, pearl millet bambaranuts and cowpea. For this reason it was embedded in<br />
the project to train farmers to produce their own seed which they can use themselves and sell to the<br />
neighbours and neighbouring villages. It is intended also that once these farmers have known the<br />
techniques of producing high quality seeds, they will later be contracted by the Agricultural Seed<br />
Agency (ASA) to produce certied seed to reach many farmers, and not within their district, but to more<br />
districts that grow the crop.<br />
Farmers were given training on the following main seed production areas:<br />
1. Organization of seed production in Tanzania (L 1): This lecture was given in order to expose farmers<br />
on the various institutions/stakeholders involved in the seed industry, where they are and what they are<br />
doing. It also intended to make them understand the seed classes produced in the country.<br />
Lecture components included the following: The roles of Agriculture <strong>Research</strong> Institutions (ARI’s),<br />
Agricultural Seed Agency (ASA), Seed companies, Farmer groups, Seed Certification Institute and<br />
Agriculture Extension Services Department, Seed production flow from ARI’s to Seed companies and<br />
to farmer groups and Seed classes recognized in Tanzania seed industry<br />
2. Attributes of seed quality (L 2): To make farmers understand the concept of seed quality and its<br />
implications in seed production, processing, storage and distribution. The lecture components included:<br />
Importance of seed as a basic input in agriculture production, Merits of improved varieties as a basic<br />
requirement for any variety before releasing to farmers and Seed quality aspects which are verifiable by<br />
already set standards by Seed Certification Institute such as genetic and physical purity, germination and<br />
seed health.<br />
3. Cowpea Seed Production (L 3): Being the core subject, farmers were trained on the techniques used<br />
in the production of cowpea seeds. More emphasis was put in the husbandry practices, which will<br />
enable them harvest high quality seed.<br />
Training components included: Land selection for cowpea seed production, Land preparation, Choice of<br />
varieties to be planted according to days to maturity, Plant spacing depending on whether a variety is<br />
determinate or indeterminate, <strong>Crop</strong> husbandry practices in general such as pest management, rouging of<br />
off types in seed production, timely harvesting, seed conditioning, safe seed storage and seed sampling<br />
and testing for quality attributes of the project should be initiated by 2010.<br />
Table 17: Summary topics covered by each Farmer <strong>Research</strong> Groups, 2009<br />
Region District Village F R G NO. of<br />
participants<br />
SINGIDA Singida<br />
district<br />
DODOMA Dodoma<br />
urban<br />
IRINGA Iringa<br />
district<br />
Topics Covered<br />
Topic Topic Topic<br />
1 2 3<br />
Msungua Mshikamano 10 X X X<br />
Ikhanoda Mfwenji 20 X X X<br />
Nduu Matumaini 21 X X X<br />
Kikombo Ushirika 11 X X<br />
Mpunguzi Twende na<br />
wakati<br />
10 X X<br />
Mkungugu Ari mpya 10 X X<br />
Mangalali Fahari 14 X X<br />
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Output 2.4: Cowpea market opportunities identified<br />
Activity 2.4.1: Market survey<br />
The survey was completed 2008 and out of it a paper was presented at the 8 th Scientific Conference of<br />
the Agricultural Economics Society of Tanzania (AGREST) held at Visitors Inn Hotel, Zanzibar, 6 th -8 th<br />
October 2008’<br />
Activity 2.4.2: Marketing chain stakeholders meeting<br />
The meeting was supposed to take place this year but due to poor cowpea harvest, farmers had nothing<br />
to offer to traders. This now has been rescheduled for next season hopping there will be good harvest for<br />
the crop as farmers have enough seed to plant during the season.<br />
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Annex 2: Cowpea Utilization in Iringa and Dodoma Regions<br />
Dr. Peter S. Mamiro; Sokoine University of Agriculture, Morogoro<br />
Dr Ambonesigwe. M. Mbwaga, ARI Uyole Mbeya<br />
Introduction<br />
Mature dry cowpeas are important in the diets of many population groups around the world. Africa<br />
alone accounts for 7.5 million hectares of the estimated world total area, of about 10 million hectares.<br />
Of the 7.5 million hectares, about 70% lies in West and Central Africa (Singh et al., 1996). This food<br />
legume is readily available, inexpensive and popular part of the traditional food system. Cowpeas<br />
(Vigna unguiculata L.Walp) along with other legumes are recognized as an important source of protein<br />
(Agazounon et al, 2004). However, their contribution to the overall diet and dietary mineral needs is less<br />
well known. For instance nutritional deficiencies of iron and zinc are often widespread in developing<br />
countries, where staple diets are frequently plant-based and consumption of expensive meat products is<br />
low. Cowpea is an annual legume, cultivated dried fresh grain and long stored dried grain being cooked<br />
while others are dehulled to remove the seed testa, then ground to obtain flour. The flour is used to<br />
make various dishes or recipes. The young and tender cowpea leaves are picked and eaten as relish<br />
along with the main staples. The grains and leaves are the source of carbohydrates, proteins, fats,<br />
vitamin A (ß-carotene), B and C, which are necessary for maintaining good health and fighting disease.<br />
Young cowpea leaves are consumed in at least 18 countries in Africa, and seven countries in Asia and<br />
the Pacific (Ahenkora et al., 1998). Cowpeas are among the top three or four leaf vegetables marketed<br />
and consumed (Barret, 1990). Cowpea also contains iron, calcium, phosphorus, magnesium, copper,<br />
zinc and is high in dietary fibre (Sinha and Kawatra, 2005). The other vegetative parts of the cowpea<br />
plant after removal of the grain, are used as feed, forage, hay and silage for livestock. This paper<br />
reports on the extent of utilization of cowpeas and its contribution to the macro and micro nutrients to<br />
communities that cultivate and consume the legume.<br />
Methodology<br />
Survey<br />
A survey to determine the pattern of utilization of cowpea was conducted in Iringa and Dodoma region<br />
in Kalenga and Msinga villages respectively. The choice of these villages was undertaken in<br />
collaboration with government extension authorities from the two regions. The communities are well<br />
known for the production, selling and consumption of cowpeas. The two regions fall within two<br />
different agro ecological zones,. Iringa being a wet region located in the southern highlands zone and<br />
Dodoma being a semi arid region in the central zone. A simple structured questionnaire seeking<br />
information on the quantity and frequency of consumption of cowpeas and other foods at the homestead<br />
was administered to 511 randomly selected farmers. Samples of local cowpea varieties and products<br />
prepared from cowpeas were collected for analysis in a laboratory at Sokoine University of Agriculture.<br />
The processing and preparation steps of cowpea recipes and products were documented.<br />
Proximate analysis of the samples<br />
The food samples for chemical analysis were grounded to pass through a one millimeter screen in a<br />
Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (% DM) was determined by<br />
drying the sample in an oven at 103 ºC – 105 ºC for 24 hours. Crude protein percentage (% CP) was<br />
determined by Kjeldahl method AOAC method No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030<br />
analyzer, whereby percentage Nitrogen obtained was used to calculate the % CP using the relationship:<br />
% CP = % N x 6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />
System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and percentage ash (%<br />
minerals) was determined immediately after the dry matter determination by incinerating the samples in<br />
a muffle furnace at 550 ºC for four (4) hours. The ash was cooled in a desiccators and then weighed.<br />
Crude fiber percentage (% CF) was determined by using the fiber system and Weende method (AOAC<br />
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1995). Nitrogen free extract percentage (% NFE) was calculated by differences as shown: % NFE = 100<br />
- (% moisture + % CP + % EE + % CF + % Ash).<br />
Total minerals<br />
Total mineral content of the samples was carried out after dry ash. The ash was dissolved in a solution<br />
of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture was 6NHCL. Total iron, zinc<br />
and calcium were determined by atomic absorption spectrophotometry by AOAC method 970.12 No.<br />
(AOAC, 1995).<br />
Data analysis<br />
Data for minerals and proximate analysis were entered in SPSS 12.0 for windows computer software.<br />
Descriptive statistics was used to determine the measures of central tendency for each analyzed cowpea<br />
variety. An analysis of variance of the results was done at 95% confidence interval (P≤0.05) using<br />
Tukeys Honestly Significant Difference. Homogeneity test was performed to determine homogenous<br />
sets.<br />
Results<br />
Demographic characteristics of farmers<br />
There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116 (48%) were<br />
females. In Dodoma 277 farmers were interviewed, 135 (49%) were males and 142 (51%) were females<br />
(Table 1). Farmer’s age ranged between 18 to 83 years with a mean of 45±15 years while in Dodoma<br />
the range was between 20 and 59 years with a mean of 35±7 years. In the two regions, the majority of<br />
the farmers had only studied up to class 7 (57% in Iringa and 88% in Dodoma) which is primary school<br />
level. However, 5% and 1% of interviewed farmers had no formal education in Iringa and<br />
Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up to six family<br />
members.<br />
Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />
Parameter Category Iringa Dodoma<br />
n % N %<br />
Gender Male 124 51.7 135 48.7<br />
Female 116 48.3 142 51.3<br />
Education Class: 3-7 164 70.4 257 92.8<br />
Class 8-14 58 24.2 16 5.9<br />
No education 13 5.4 4 1.4<br />
Household size 1-6 members 192 80.8 183 66.8<br />
7-14 members 48 19.2 94 33.2<br />
Cowpea harvested, procured and utilization<br />
Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own cowpea, or obtained<br />
cowpea supplies from friends and relatives. Few of the farmers purchased cowpea for home<br />
consumption (Table 2). Farmers harvested an average of 75 kg of cowpeas per household with a range<br />
from 53 to 118 kg and the amount sold per household ranged from 4 to 73kg with an average of 25kg<br />
per household. The amount kept at home for consumption ranged from 36 to 97 kg with an average of<br />
52 kg. A substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />
between 100 and 1000 grams of cowpea leaves for household consumption depending on the household<br />
size. Per capital consumption ranged from 40 to 200 gm of cowpea grain for 84% of households in<br />
Iringa and 94% in Dodoma. At the same time per capital consumption for cowpea leaves ranged from<br />
10 to 500 grams by 95% of households in Iringa and 100% in Dodoma, respectively.<br />
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On days when cowpeas are cooked in a household, the same relish will be eaten with either rice or stiff<br />
porridge during lunch, dinner and with breakfast the following morning. More than 50% of households<br />
in Iringa and Dodoma consumed cowpea at least once and up to three times a week (Table 2). Cowpea<br />
leaves were also prepared and eaten on separate days. The leaves are consumed fresh especially during<br />
wet season and in dried form during the lean or dry season. Almost all farmers in the two regions<br />
consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff porridge (ugali), a<br />
mixture of dehulled maize and cowpeas (kande) and most frequently the cowpea buns (bagia).<br />
Table 2: Farmers Responses on Cowpeas Utilization<br />
Parameter Category Iringa Dodoma<br />
n % n %<br />
Ever eaten cowpea Yes 240 100 277 100<br />
How often eaten per day Twice 92 38.5 156 56.3<br />
Thrice 147 61.5 121 47.7<br />
How often eaten per week Once 77 32.1 28 10.1<br />
Twice 75 31.3 119 43.0<br />
Thrice 65 27.1 82 29.6<br />
Four Times 11 4.6 38 13.7<br />
Five Times 12 5 10 3.6<br />
Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />
Purchase<br />
market<br />
from the 41 17.1 6 2.2<br />
Get from the neighbor 75 31.3 140 50.5<br />
How much cowpea is prepared in one 100-500gms<br />
meal<br />
137 57.1 115 76.9<br />
500-2000gms 103 42.9 64 33.1<br />
How much cowpea leaves are prepared<br />
in one meal<br />
100-1000gms 146 61.9 227 89.7<br />
1000-4000gms 90 39.1 26 11.3<br />
Consumed green cowpea leaves Yes 240 100 277 100<br />
Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />
Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />
20 – 90 minutes 133 65.7 214 79.4<br />
Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />
20 – 90 minutes 31 54.4 72 62.1<br />
How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />
Wash-chop-cook 214 90.7 44 44<br />
Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />
Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />
Cowpea roasted 17 2.1 44 4.8<br />
Roasted milled to flour to prepare<br />
porridge<br />
Cowpea Porridge 28 3.5 66 7.2<br />
Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />
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Parameter Category Iringa Dodoma<br />
Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />
Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />
201-500 39 16.2 19 7.0<br />
Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />
500 - 1400 12 5.1 0 0<br />
Mineral Content<br />
Minerals of nutritional importance that were analyzed included; iron, zinc and calcium. Analysis<br />
showed that all varieties had significantly different (p
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />
Moisture<br />
(%)<br />
27<br />
Ash<br />
(%)<br />
Ca<br />
(mg/kg)<br />
Zn<br />
(mg/kg)<br />
Fe<br />
(mg/kg)<br />
Local Cowpea grain Dodoma 9.40 3.89 958.14 31.45 28.90<br />
Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />
Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />
Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />
Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />
Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />
Local Cowpea grain Iringa 9.56 3.66 992.43 32.56 27.61<br />
Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />
Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />
Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />
Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />
Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />
Proximate composition<br />
Proximate analysis showed that the cowpea varieties with the highest crude protein content were<br />
IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%) Varieties with lowest iron<br />
included IT00K-1207 (22.01%) and TZA 263 (22.6%). For fats, IT97K499-8 (11.18%), IT99K-7212-2-<br />
1 (10.98%) and Fahari (10.33%) varieties had the highest levels while the lowest included IT97K819-<br />
118 (8.17%) and IT96D-733<br />
(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118 (16.1%). The<br />
lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%) and VULI 2 (13.1%). Among<br />
the best new lines with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1.<br />
Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />
Variety/Food component % DM %CP %CF %EE %NFE<br />
VULI 1 89.25 24.02 13.77 9.12 38.18<br />
IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />
IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />
IT96D-733 88.54 23.44 14.91 8.34 37.68<br />
IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />
IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />
FAHARI 89.29 25.46 13.45 10.33 35.86<br />
TUMAINI 89.13 25.96 13.83 9.89 35.23<br />
IT00K-1207 87.95 22.01 15.66 8.46 37.82<br />
IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />
TZA263 89.21 22.60 17.21 8.86 36.45<br />
VULI 2 89.53 25.64 13.10 10.25 36.53<br />
IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />
B301 90.11 23.75 14.85 8.39 38.85<br />
IT99K-721-2-2-1 89.68 26.00 12.53 10.98 35.89<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />
Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />
Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />
Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />
Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />
Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />
Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />
In comparison with the improved lines and varieties, local cowpeas have comparable levels of dry<br />
matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table 5). However, the levels of<br />
fats are relatively lower (5.4%) in comparison with the improved lines/ varieties ranging from 8% to<br />
11.2%. A similar trend is shown with cowpea leaves. Dehulling the cowpeas reduces crude fibre<br />
significantly with a gradual fall in fats. With regard to the cowpea buns from Iringa and Dodoma<br />
regions there was significant drop in crude protein and crude fibre but a very significant rise in fats<br />
(14.3%).<br />
Discussion<br />
Macro and micro nutrient content<br />
In the two surveyed regions it was revealed that cowpeas are produced by majority of the farmers and<br />
consumed to a relatively large extent by all respondents, which provides an indication of cowpea<br />
importance in the two communities. Similarly, the survey revealed that cowpeas are prepared and<br />
consumed in a number of ways, such as relish for stiff porridge and rice, mixed and boiled with dehulled<br />
or un-dehulled maize and cowpea buns. In both regions, the cowpea buns were important as they were<br />
also a source of income in a number of households. The buns however, appear to have lower iron and<br />
calcium, especially those from Iringa region. The calcium, zinc and iron content of the cowpea buns<br />
were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma region had comparatively<br />
higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and iron (16.3mg/kg). This difference might<br />
emanatre from the method of preparation of cowpeas in Iringa in comparison to Dodoma. In Iringa the<br />
grains are dehulled and soaked for about three hours and then washed in flowing river water for about<br />
an hour. This procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />
these minerals are largely lost during washing.<br />
With regard to the deep frying of the cowpea buns, though some nutrients such as proteins and crude<br />
fibre were significantly reduced (from average of 22% to 19% protein), in the process fats were<br />
significantly increased (from average of 5% to 14%), while nitrogen free extracts and dry matter<br />
remained stable. The oil increase is attributed to the sunflower oil used to deep fry the buns. The farmers<br />
also cultivate sunflower for home use and sale. Greater oil intake is better as daily activities require<br />
adequate energy supply.<br />
Analysis of the developed cowpea varieties showed relatively higher protein levels of 24- 26% and 8%-<br />
11% fat content. Similar results have been observed by Horax et al., (2004) where they found cowpea<br />
seed contained 20-25% protein and was rich in essential and non-essential amino acids. Asante et al.<br />
(2006) were investigating on 32 accessions of cowpea seeds, found protein and tannin contents ranging<br />
from 16.4-27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006) proximate<br />
analysis of various infested cowpea samples indicated increases in protein and crude fiber contents and<br />
decreases in moisture, ash, fats/oil and carbohydrates contents when compared with those of the<br />
uninfected samples. Percentage increases in protein and fiber contents ranged between 9.31-30.56%,<br />
and 1.24-15.11%, respectively while percentage decreases were, moisture (3.97-10.23%); ash (3.75-<br />
12.87%); fats/oil (1.43-10.00%); and carbohydrate (1.88-7.50%).<br />
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Similarly the developed cowpeas were relatively high in calcium, iron, and zinc. However,<br />
considerable mineral variation was found among the analyzed cowpeas varieties. Similar study by<br />
Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea variety with the highest calcium<br />
concentration of 2096.0 g/g and Zn was detected in only few varieties ranging from 1501.0 g/g to<br />
2071.0 g/g. Analysis of variance of the samples showed significant differences (p
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed varieties,<br />
which are higher yielding and resistant to Alectra, they will probably be accepted by the communities<br />
and the amount per capita of cowpeas consumed should increase provided they continue to cultivate the<br />
same area of land.<br />
Conclusion<br />
Cowpea grain and leaves are widely consumed in the two surveyed areas. The contribution of micro and<br />
macro nutrients is significant for both developed lines and local varieties but with leaves having greater<br />
mineral content than grain. Therefore awareness-raising within communities to consume more cowpea<br />
leaves is required. However the amount produced per household is low compared to requirements.<br />
Consequently cowpea intake per capita is low, and does not meet the RDA. However, since households<br />
in both areas still have ample land they should be encouraged to increase the area planted as well as<br />
planting higher yielding varieties in conjunction with preferred local varieties. This does however<br />
require further market investigation.<br />
Other important nutrients such as vitamins, amino acid profile and levels of ant-nutritional factors<br />
(tannins and phytates) should also be further investigated. The vitamins and amino acids influence in<br />
nutrition while the antinutritional factors influence on the bioavailability of the nutrients in the body.<br />
Acknowlegement<br />
The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong> <strong>Foundation</strong>, who<br />
facilitated successful survey and laboratory analysis of macro and micronutrients.<br />
References<br />
Agazounon, C., Coulibaly, O. and Houndekon, V. (2004) Analysis of of cowpea processing techniques<br />
into ‘atta’ in Benin. Bulletin de la recherché Agronomique du Benin. No. 46, September 2004.<br />
Ahenkora, K. Adu Dapaah, H. K. and Agyeman, A. (1998) Selected nutritional components and sensory<br />
attributes of cowpea (Vigna unguiculata [L.]Walp) leaves Plant Foods for Human Nutrition 52:<br />
221–229.<br />
AOAC (1995), Official methods of analysis. Association of Official Analytical Chemists methods,<br />
AOAC 16 th edition Nos. 963.13, 968.08, 970.12, 915.03, 920.87. Washington, D.C.<br />
Asante, I. K., Adu-Dapaah, H Addison (2006) Seed weight and protein and tannin contents of 32<br />
cowpea accessions in Ghana. Journal of Tropical Science, 44 (2): 77 – 79.<br />
Barret R. P. (1990) Legume species as leafy vegetables. In: Janick J, Simon J. E. (eds), Advances in<br />
new crops. Portland, OR: Timber Press, pp 391–396.<br />
Horax, R., Hettiarachchy, N. S., and Jalaluddin M. (2004).Preparation, characterization and functional<br />
properties of protein isolate from cowpea (Vigna unguiculata L. Walp.) Dept. of Food Science,<br />
Univ. of Arkansas, 2650 N. Young Avenue, Fayetteville, AR AR 72704, (2) Department of<br />
Agriculture, University of Arkansas, Pine Bluff, AR 71611.<br />
http://ift.confex.com/ift/2004/techprogram/paper_22759.htm.Accessed 19.09.2009.<br />
Mgbemena, I. C., Opara, F, N. and Iwuala, M. O. (2006) The effect of insect pest infestation on the<br />
nutritional composition of cowpea and maize. Department of Biotechnology, Federal University<br />
of Technology PMB 1526 Owerri, Nigeria International Journal of Natural and Applied<br />
Sciences 2 (2): 96-100, 2006.<br />
Mune Mune, M. A., Mbome, L. I. and Minka S. R. (2007) Improving the Nutritional Quality of Cowpea<br />
and Bambara Bean Flours for Use in Infant Feeding, Pakistan Journal of Nutrition 6 (6): 660-<br />
664<br />
Olaleke, A. M., Olorunfemi, O. and Akintayo, T. E. (2006) Compositional evaluation of cowpea (Vigna<br />
unguiculata) and scarlet runner bean (Phaseolus coccineus) varieties grown in Nigeria.<br />
International journal of food, agriculture and environment 4 (2): 39‐43.<br />
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Singh B. B., Sharma B. M. and Chambliss O. L. (1996). Recent advances in cowpea breeding. In<br />
Proceedings of the Second World Cowpea <strong>Research</strong> Conference, 5–8 September 1995, Accra,<br />
Ghana. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.<br />
Sinha, R. and Kawatra A. (2005) Effect of Processing on phytic acid and polyphenol contents of<br />
cowpeas (Vigna unguiculata (L) Walp. Plant Foods for human Nutrition, 58 (3): 1‐8.<br />
Wang, N., Hatcher, D.W., Toews, R. and Gawalko, E.J. (2009) Influence of cooking and dehulling on<br />
nutritional composition of several varieties of lentils (Lens culinaris) LWT ‐ Food Science and<br />
Technology, 42, (4): 842‐848.<br />
Weinberger, K., John Msuya, J. and Mamiro, P. (2004) Domestication of selected African indigenous<br />
vegetables in Tanzania – an ex‐ante impact assessment. AVRDC – The World Vegetable Center,<br />
pp22.<br />
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Annex 3: Malawi annual report:<br />
Malawi annual report 2008-09 season (third year)<br />
Prepared by V.H. Kabambe, E. Kazila, A. Mangwela, J Bokosi<br />
CC Mtambo, T. Chilongo and E. Mazuma<br />
1. Field evaluation of promising varieties<br />
1.1 Sites and entries.<br />
On farm trials to evaluate performance and adaptation of outstanding lines were conducted in the<br />
2008/09 season. Trial sites were Bunda College in Lilongwe, Mgwangwa EPA in Lilongwe, Mpokwa<br />
EPA in Zomba, Rivirivi EPA in Balaka, Chitedze <strong>Research</strong> Station and Kasungu. Kusungu and Balaka<br />
are new sites which were added in order to consolidate the genotype x environment evaluation, which<br />
would provide strong evidence on adaptation zones for any new entry which we may have to formalize<br />
for release. Balaka is a low altitude site with low and erratic rainfall. Kasungu is a mid-altitude site<br />
with sandy soils. The rainfall was normal and fairly distributed for all sites (Table 1).<br />
Table 1. Mean monthly rainfall (mm) for the trial sites in 2008/09 season.<br />
Month Site<br />
Bunda Ngwangwa Rivirivi Chitedze<br />
October ‘08 18.5 0 3.0 45<br />
November ‘08 119.2 51 113.4 112<br />
December ‘08 177.1 147 242.1 116.4<br />
January ‘09 291.9 198 300.5 227.8<br />
February ‘09 174.0 152 145.1 121<br />
March ‘09 218.3 134 125.1 223.7<br />
April /09 28.0 71 6.3 18.1<br />
May ‘09 0 0 0 0<br />
Monthly total 1026.7 753 932.5 864<br />
Seven of entries selected for evaluation based on Alectra resistance, farmer’s preferences on grain<br />
characteristics and according to evaluations conducted in the last two years. Table 2 gives brief<br />
highlights about the entries based on preliminary yield evaluations. There were about 25 farmers at 4<br />
on-farm sites conducting the trial, each farmer acted as a replicate. At Chitedze and Bunda College the<br />
trials were complete block design, each with 4 replicates. The trial plots had 5 row plots, 4 m long and<br />
0.75 m apart.<br />
Table 2: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />
Code Name Description<br />
5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra<br />
vogelii resistance<br />
37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />
preference<br />
9 IT99K-7-21-2- Best Alectra resistance in ’07, ‘07/08, preferableseed size, , good<br />
2-1<br />
yield<br />
21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium<br />
seed size, low farmer preference<br />
53 Sudan -1 Released variety, good resistance to Alectra and good yielder in<br />
‘07/08<br />
52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08.<br />
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Poor resistance to A. vogelii. Well liked by farmers.<br />
54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />
1.2 Screening for Alectra resistance.<br />
The sites at Ngwangwa and Kasungu had high infestation of Alectra, even though yields were good..<br />
However, within the plots, spots that had high numbers of Alectra were causing stunted growth and<br />
wilting on cowpea plants. Observations on Alectra resistance showed that in sites with high pressure, all<br />
entries succumbed to the parasite, though differentilly. In lightly infested sites, some entries showed<br />
resistance (Table 3). .At Mngwangwa, where pressure was high, the same lines showed fair resistance.<br />
At Lisasadzi, with high Alectra pressure, entries IT97K-825-15 and IT99K-494-6 showed good<br />
resistance. At Bunda College, with high Alectra pressure, the same three lines showed resistance. On the<br />
overall, entry 21 (IT99K-494-6) and entry 9 (IT99K-7-21-2-2-1) were best for resistance with 0.28 and<br />
0.29Alectra plants m -2 respectively, and entry 21 having zero Alectra count at Bunda. Depending on<br />
yield and other results, one of these three could be released. However, all these have longer maturity<br />
periods.<br />
As these observations were done in presence of local extension and farmers, this gave a good<br />
opportunity to orient farmers to the Alectra problem and the objectives of the project. Since the entries<br />
in the trial were combination of early and late maturing types, the general farmers preference at all sites<br />
was for the early types IT82E-16 and Sudan 1. At Kasungu farmers particularly noted that IT97K-825-<br />
15 was undesired due to its late maturity, as it would need more rains. Some of the other reasons for<br />
preferences were grain size (large size preferred).<br />
Table 3 Alectra counts m -2 taken after flowering at all the trial sites<br />
Entrycode<br />
name<br />
& Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />
5=IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />
37=IT97K-825-<br />
15<br />
0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />
9=IT99K-7-21-2-<br />
2-1<br />
0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />
21=IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />
53=Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />
52=IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />
54=Farmers’<br />
local<br />
2.80 1.14 3.29 - 0.03 0.11 1.23<br />
Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
traits in entry 9 and 21 can be reflected in the progenies. The project is so far promoting the released<br />
varieties into its outreach progammes.<br />
Results on number of plants/m 2 are shown in Table 5and they show that there were significant<br />
variations in plant establishment. Looking at yield data, entries 21, 52 and 53 which gave highest yields<br />
also had the best plant establishment.<br />
Table 4:. Grain yield kg/ha of cowpea entries at 6 trial sites in 2008/09.<br />
Entry Site Mean<br />
Lisasadzi Mpokwa Bunda Balaka Mgwangwa Chitedze<br />
5=IT98K-503-1 2034 461 1496 749 856 1667 1210<br />
9=IT97K-7-21-2-<br />
2-1<br />
1749 353 1665 903 543 1222 1073<br />
21=IT99K-494-6 2345 562 1846 1372 1114 972 1369<br />
37=IT99K-825- 1527 447 1276 589 796 1083 953<br />
15<br />
52=IT82E-16 1936 896 1631 2156 903 3194 1786<br />
53=Sudan-1 2328 1402 1728 2158 1070 2278 1827<br />
54=Local - 353 794 964 536 1861 902<br />
Mean 1936 681 1492 1270 831 1754 1327<br />
LSD 523 544 830 878 435 1340<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
9=IT97K-7-21-2-<br />
2-1<br />
10.4 9.0 13.0 10.5 10.0 8.6<br />
21=IT99K-494-6 10.2 11.0 10.8 10.7 11.1 10.8<br />
37=IT99K-825-<br />
15<br />
8.8 10.5 10.7 10.2 10.6 10.2<br />
52=IT82E-16 13.8 13.4 11.5 15.0 14.1 13.5<br />
53=Sudan-1 16.2 17.0 14.8 17.2 16.1 16.3<br />
54=Local - - 16.0 16.0 10.0 14.0<br />
Mean 11.3 11.5 12.3 12.7 12.3 12.0<br />
P
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
54=Local 3.0 3.5 3.2 4.8 5.0 4.9<br />
Mean 2.5 3.4 3.0 3.7 5.9 4.8<br />
P 0.43 0.60 0.43 0.09<br />
LSD 0.88 1.0 2.40 1.7<br />
CV% 24 21 44 19<br />
Table 9. The effect of genotype on early and late season Cercospora leafspot Incidence (5 plants<br />
affected) and scores (scale 1=clean, 9=severe) of Chitedze <strong>Research</strong> Station.<br />
Entry Cercospora Incidence Cercospora leafspot infection<br />
Early Late Mean Early Late Mean<br />
5=IT98K-503-1 26.6 72.5 49.6 4.2 6.5 5.4<br />
9=IT97K-7-21-2-2-1 21.3 73.8 47.6 5.8 7.3 6.5<br />
21=IT99K-494-6 27.3 68.9 48.1 5.0 7.0 6.0<br />
37=IT99K-825-15 20.8 73.2 47.0 4.8 6.8 5.8<br />
52=IT82E-16 19.2 64.4 41.8 4.8 6.0 5.4<br />
53=Sudan 27.2 75.2 64.4 5.3 7.2 6.3<br />
54=Local 32.7 70.2 75.2 5.8 6.2 6.0<br />
Mean 25.0 71.2 48.1 5.1 6.7 5.9<br />
P 0.38 0.80 0.44 0.14<br />
LSD 13.3 15.2 1.6 1.0<br />
CV% 36 23 22 11<br />
1.5 Days to flowering and maturity<br />
The variations of cowpea entries on days to 50% flower and to maturity are shown in Table 10 and 11.<br />
Table 10 shows that on average plants flowered earliest at Mpokwa (46 days from planting), closely<br />
followed by Mngwangwa (47 days). Mean date to flowering was most delayed at Lisasadzi in Kasungu,<br />
mainly due to the influence of the local variety which took 89 days to flower. In general Sudan-1 was<br />
the earliest to flower across all sites, followed by IT82E-16. The local entry was the last to flower at<br />
Mngwangwa and Lisasadzi only. In general, there were small differences between flowering dates of the<br />
improved varieties (no more than 10 days), but the farmers highlighted that these differences were<br />
significant and important. Regarding days to maturity, the mean maturity period was shortest at<br />
Mpokwa and Balaka, which was expected. These sites are warmer and drier historically. Apart from<br />
the local entry, entries 9 and 37 had the longest maturity periods of about 77 days. This agrees with<br />
observations in the fields and farmers reasons for disliking the entries. Although these entries were late,<br />
they were inferior in yield compared to the early maturing released varieties. This further suggests good<br />
adaptability for the released varieties.<br />
Table 10. Effect of genotype on days to 50% flower at 5 sites, 2008/09<br />
Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />
5=IT98K-503-1 46 52 57 46 48 50<br />
9=IT97K-7-21-2-2-1 48 54 59 53 53 53<br />
21=IT99K-494-6 46 52 57 46 47 50<br />
37=IT99K-825-15 47 56 58 53 52 53<br />
52=IT82E-16 44 48 55 39 46 46<br />
53=Sudan-1 46 47 59 41 51 49<br />
54=Local 50 53 89 - 52 61<br />
Mean 47 52 62 46 50 51<br />
P
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
CV% 4 4 9 7 26<br />
Table 11 Effect of cowpea line on days to maturity at five sites, 2008/09 season.<br />
Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />
5=IT98K-503-1 79 82 73 64 61 72<br />
9=IT97K-7-21-2-2-1 80 83 76 73 70 76<br />
21=IT99K-494-6 80 82 71 65 60 72<br />
37=IT99K-825-15 83 88 72 74 69 77<br />
52=IT82E-16 74 75 60 59 66 67<br />
53=Sudan-1 74 74 68 60 69 69<br />
54=Local 80 83 107 - 68 84<br />
Mean 78 81 76 66 66 73<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Table 13. Proportion of farmers preferences of trial lines based on selected criteria<br />
Entry Criteria Site or village<br />
Bala Bunda Mngwa Mpokwa Mpokwa Lisaka<br />
ngwa Khuvinda CChibisa Sadzi<br />
vge Vge<br />
n 24 40 17 40 39 32<br />
5=IT98K-503-1 Seed size 4 100 100 90 10 34<br />
Plant type 0 100 100 5 0 59<br />
Early maturity 0 100 100 0 100 3<br />
Insect resistant 0 100 100 0 0 28<br />
Disease resistant 0 100 100 0 25 38<br />
9=IT97K-7-21-2-2-<br />
1<br />
Seed size 100 100 65 60 100 53<br />
Plant type 100 40 41 35 0 63<br />
Early maturity 0 100 47 0 0 0<br />
Insect resistant 71 100 100 0 0 0<br />
Disease resistant 71 100 100 0 0 0<br />
21=IT99K-493-6 Seed size 100 25 71 55 60 50<br />
Plant type 4 0 88 0 26 81<br />
Early maturity 100 0 41 45 67 100<br />
Insect resistant 0 0 100 0 44 100<br />
Disease resistant 0 0 100 0 21 63<br />
37=IT99K-825-15 Seed size 100 70 0 100 38 0<br />
Plant type 0 100 41 15 0 44<br />
Early maturity 0 83 0 0 0 0<br />
Insect resistant 0 100 100 25 0 0<br />
Disease resistant 0 100 100 15 0 13<br />
52=IT82E-16 Seed size 71 35 91 75 28 59<br />
Plant type 100 40 100 100 49 63<br />
Early maturity 100 55 100 100 62 81<br />
Insect resistant 0 60 100 100 40 57<br />
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2009<br />
Disease resistant 0 50 100 100 26 100<br />
Seed size 75 83 88 75 21 82<br />
53=Sudan Plant type 100 100 100 100 49 68<br />
Early maturity 100 100 100 100 59 100<br />
Insect resistant 100 100 100 100 59 100<br />
Disease resistant 100 15 100 100 77 59<br />
Farmers local Seed size 100 50 0 65 39 19<br />
Plant type 100 0 0 100 41 19<br />
Early maturity 0 75 0 0 0 0<br />
Insect resistant 0 0 0 100 72 0<br />
Disease resistant 0 0 0 100 62 0<br />
1.7 GENERAL DISCUSSION<br />
The results of the field test have been informative due to close association between yield data, yield<br />
components and agronomic traits. The interesting point is that the earlier maturing released varieties<br />
gave highest yields. In general, longer maturity varieties are expected to give higher yields, the reason<br />
for low yields could be low yield potential, or uncontrolled insect pest attack. The key observations on<br />
the entries are summarized in Table 14 below. All entries were equally prone to pests and diseases. The
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
high yields recorded under famer’s conditions are encouraging for them to grow cowpea for food and<br />
income generation.<br />
Table 14. Key traits and strengths of trial entries<br />
Entry Key traits and strength<br />
5=IT98K-503-1 Low yielder, medium maturity (64 days), disease prone, poor farmer<br />
preference, medium Alectra resistance<br />
9=IT97K-825-15 Large seed size, low yielder, good Alectra resistance, medium farmer<br />
preference, long maturity period (73 days), disease and pest prone<br />
21=IT99K-7-21-2-2- Good seed size, medium maturity (65 days), very good Alectra resistance,<br />
1<br />
prone to pests, earmarked for release.<br />
37=IT99K-494-6 Poor farmer preference, late maturity (74 days), very good Alectra<br />
resistance, poor yielder, prone to disease and insect pests<br />
52=Sudan-1 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />
maturity, released variety<br />
53=IT82E-16 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />
maturity, released variety<br />
54=Local Variable traits depending on source, largely late maturing, pest prone,<br />
medium yields<br />
2. INCORPORATION OF ALECTRA RESISTANCE INTO ADAPTED VARIETIES<br />
This work is in progress. The released lines are being upgraded to incorporate Alectra vogelii resistance.<br />
The donor lines for Sudan-1 are IT97K-820-18, IT99K-1060, IT99K494-6, IT97K-7-21-2-2-1, and<br />
IT93K-452-1. The donor linefor IT82E-16 is 1T81D-994. The work is at backcross 2 stage to be<br />
followed by selfing.<br />
3. COLLABORATIVE STUDENT WORK<br />
3.1 BSc student (Cowpea variety x time of planting x density trial)<br />
During the season an undergraduate student undertook a study on cowpea time of planting x variety x<br />
density trial. The objective was to determine how these factors interact to optimize crop yields with<br />
respect to rainfall and the need to plant late to avoid disease and insect pressure. Preliminary<br />
observations showed that early planting was the best for yield and less disease infection. This was<br />
because there was a dry spell soon after the first planting. This might have led to conditions not<br />
favourable for the build up of the diseases.<br />
3.2: MSC Student (Ms Elida Kazira - Farmer Participatory Selection of Alectra Resistant Cowpea<br />
Varieties in Selected Districts of Malawi).The student has submitted her thesis which is in the hands of<br />
examiner.<br />
4. CAPACITY BUILDING AND SCALING OUT OF IMPROVED COWPEA LINES<br />
4.1 Primary seed support<br />
The project provided small packs of seed of 0.5 kg each to all project participating farmers Mpokwa and<br />
Mngwangwa EPA (about 60 farmers at each EPA). Participating farmers in Kasungu were also given<br />
this support. Many farmers planted this in pure stand while others planted this in intercrop with maize.<br />
The objective of this program was to provide an experience for the farmers to grow cowpea intensively<br />
(as sole crop) and to practice pest management and observe results. Some of the farmers we monitored<br />
managed to get good yields and were motivated to produce cowpeas. We have helped these farmers to<br />
store some of their seeds so that they can plant more cowpeas next season. During field days and visits<br />
farmers were trained on principles of cowpea seed production. Farmers were also encouraged to use<br />
their cowpea to practice the different recipes which they learned from the project.<br />
4.2 Agriculture fair participation and sharing of cowpea recipes<br />
In June 2009 the farmers from Mngwangwa EPA, on their own accord, organized themselves to<br />
participate at a District Agriculture Fair held in the same EPA. The farmers demonstrated a range of<br />
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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
cowpea products based on the training made by the project and shared this with participants (Figure 1).<br />
In addition, the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science<br />
Department. Again, the focus of the exhibit was of utilization. The exhibits attracted a lot of attention<br />
from participants.<br />
Figure 1. Farmer group displays of cowpea recipes (left) and cross section of participants (right)<br />
at ADD – wide show in Lilongwe.<br />
4.3 Group dynamics training for farmers in Ngwangwa<br />
Group dynamics training was conducted for all the farmers in the two participating villages in<br />
Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />
leadership and operation. This group strengthening included membership and responsibilities,<br />
constitution, networking and fund raising to sustain the groups.<br />
4.4 Workshops on cowpea market value chain<br />
Cowpea market value chain workshops were conducted in Lilongwe and Zomba in August 2009. These<br />
workshops were held following the conclusion of a market survey. As per project document, the,<br />
meetings were held to bring together representatives of cowpea producers, middlemen and traders. The<br />
objective was to link producers better to the market and identifies increased marketing opportunities.<br />
There were lead presentations made by project team theme leaders as follows:- a) a presentation on<br />
cowpea production trends, production requirements, cowpea agronomy, potential yields reasons for low<br />
yields in Malawi and cowpea storage Mr E. Mazuma; b) a presentation on cowpea utilization, including<br />
nutritional value, processing of leaves and grain, utilization as animal feed by Dr Mangwela and c)<br />
presentation on cowpea preferences and marketing by Mr Thabie Chilongo. At both venues there were<br />
representatives of farmers, government extension, NGO’s, agricultural input suppliers, traders and<br />
researchers.<br />
The workshop revealed that there are six big business traders who buy cowpea seeds (ADMARC, Rab<br />
Processors, Mulli Brothers, Agora, Grain Traders Association, and Transglobe). Many of them placed<br />
emphasis on large seeded grain types as preferences. A representative of ADMARC indicated that this<br />
season they have been instructed to buy large seeded cowpea only. The traders acknowledged that they<br />
don’t buy much cowpea, but that the markets were small as well thus can satisfy it. Some cowpea is<br />
sold locally (for cakes and cookie making) while most of it is exported. However, it was noted that most<br />
of the cowpeas are traded in local markets with no clear records on volumes and quality requirements.<br />
At the meetings we displayed a draft version of our booklet on cowpea production and utilization. The<br />
participants demanded speedy release of this book. The nutrition officers collected the draft copies for<br />
immediate use. The workshops also noted that there are many reasons for variety choice amongst<br />
40<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
farmers, such as maturity period, plant stature (whether climbing or creeper), seed size, stay green<br />
characteristics (for prolonged leaf harvesting), seed size and pest resistance. Participants resolved that<br />
some of these preferences were minor and suitable for backyard crop for home consumption. It was thus<br />
resolved that researchers should focus on high yield and pest resistance and commercially desirable<br />
traits such as large seeds. Farmers should be advised to plant small patches of local varieties for<br />
vegetables and green pods.<br />
At the Lilongwe Workshop the research team established links with IITA, which has just won a grant to<br />
work on cowpea. The initial collaboration will cover scaling out issues and market and preferences<br />
surveys. At the Zomba workshop we established a partnership with World Vision Area Development<br />
project officer to facilitate seed supply for groups of farmers for scaling out. They already have<br />
experience on community-based seed production. Similar relationships were established with the<br />
District Agriculture Development Officer for Phalombe.<br />
4.5 Cowpea market survey<br />
This work has been conducted and results are being analyzed, a separate report will be prepared. Very<br />
preliminary results were shared during workshops on cowpea market value chain.<br />
4.6. Policy workshop on seeds<br />
The Malawi country lead participated in a workshop bringing together relevant policy officials to<br />
discuss issues that can facilitate the scaling out of legume seed systems and Integrated Soil Fertility<br />
Management. The workshop was jointly organized by the <strong>McKnight</strong> Legume Best Bets and Seed<br />
Systems projects.<br />
The major policy issue on seed systems, which was a matter of concern, was the seed registration and<br />
certification process. The key concern was t based on the fact that process is too centralized. A seed<br />
grower is required to register at Chitedze <strong>Research</strong> Station, and only the same can certify. The<br />
registration fee is high, particularly for small holder farmers or community group whose cultivated area<br />
is 1-2 ha. The registration fees and inspection costs will erode profits. The argument was that legumes,<br />
self pollinated, have simple requirements for isolation, and rouging for disease and off-types can be<br />
done at District level. Any seed not going through the process cannot be packed and sold on shelves,<br />
then killing any entrepreneurship. The dissemination of legume seeds is directly linked ISFM because<br />
the same do make some N additions to soil. These concerns and other suggestion were raised for<br />
consideration by policy makers.<br />
5: Way forward<br />
Based on the above, the project team would like to make the following recommendations for future<br />
research action.<br />
1. Evaluate the lines again this season and earmark entry 21 for release owing to its good Alectra<br />
tolerance and medium yield<br />
2. Noting the lack of resistance to disease and pests across lines, to intensity the work on crop<br />
protection. This work should include clear definitions of critical minimum sprays required to<br />
keep pesticide costs and labour down. Also to intensity studies on use of botanical pesticides<br />
such as neem, moringa, Tephrosia vogelii and others.<br />
3. To refine the issues of time of planting in line with variety maturity periods in order to avoid<br />
pests and for drought management purposes.<br />
4. To intensify incorporation of resistance into the adapted lines and conduct adaptation trials.<br />
5. To extend primary seed support programme to all testing sites and to willing partners.<br />
6. To request for extension of the project with the objective of completing tasks above. To release<br />
new Alectra resistant at the end of renewal, as well as to establish a seed base to support the<br />
scaling out of the new varieties.<br />
41<br />
2009
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
42<br />
2009<br />
7. Continue with introgression of Alectra resistance in adapted cowpea varieties at Bunda College<br />
if extension of the project has been granted. At the end of the first phase we shall have reached<br />
backcross four.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />
Team Report<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Project leader: Dr AM Mbwaga, ARI Uyole, Box 400 Mbeya, Tanzania. Email: ambwaga@yahoo.co.uk<br />
Team report for 2008 ‐ 2009<br />
Team activities<br />
Team members from Malawi and Tanzania had a number of opportunities to meet during the year.<br />
These included the CoP2 meeting in Maputo, Mozambique in October 2008. This event allowed<br />
representatives from each country to discuss progress and were followed up with a visit by the project<br />
leader Dr Mbwaga accompanied by Dr Mligo, legume breeder, Dr Charlie Riches (CoP- liaison officer) to<br />
Lilongwe in March 2009 and met by the Malawian project team that was composed of Dr Vernon<br />
Kabambe country project leader, Mosses Mamiliro (breeder), and James Bokosi (breeder). The team<br />
visited several project sites, which included Lilongwe district in Ngwangwa Extension Planning Area<br />
(EPA), Kasungu district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi<br />
EPA where farmer groups are undertaking on‐farm trials with the project. The wrap up discussions at<br />
Bunda College, University of Malawi came up with the following observations/recommendations; -<br />
released varieties showed better yields than test lines, early maturing varieties save as source of food in<br />
hunger periods, seed system for cowpea is very poor, more cowpea seed should be sent to Balaka and<br />
Kasungu districts as there was high demand for seed of the improved cowpea varieties and lastly farmers<br />
in Rivirivi were recommended to form farmer research groups for better access by researchers and<br />
extension staff as compared to individual isolated farmers<br />
Representatives of project partner institutions travelled together to assess progress with project<br />
activities in Tanzania starting on 27 th April, 2009 in Iringa and ending on 30th April at Ilonga Agricultural<br />
<strong>Research</strong> Institute, Kilosa in Tanzania. Evaluation team members were: Joseph Mligo (Breeder, ARI<br />
Ilonga Kilosa Tanzania); Vernon Kabambe (co‐ordinator for Malawi, Bunda College Malawi); James<br />
Bokosi (Breeder, Bunda College of Malawi), Boukar Ousmane (cowpea breeder IITA Kano Nigeria) and<br />
Ambonesigwe Mbwaga (Project leader, ARI Uyole Mbeya Tanzania). The team was joined by Extension<br />
staff working with the project and other staff members at various sites in each district of Tanzania. From<br />
the evaluation it was noted that drought (rainfall, poor distribution and low amounts) has been an<br />
overriding problem this season. The team commended the collaboration the project is undertaking along<br />
with the tropical legumes II project of IITA. It was recommended to incorporate both traits (Alectra<br />
resistance and Drought tolerance) in the breeding programme. An analysis of medium term climatic data<br />
(20-25 years) would be important to determine the frequency of rainfall events (e.g. start dates, dry spells<br />
and rainfall amounts). This can help to determine the important drought traits in plants (e.g. earliness or<br />
resilience). The good production practices should be incorporated in the screening of the varieties against<br />
Alectra. Hence, there is a need to expose the farmer groups involved in the project to all these production<br />
practices.<br />
The experiences observed from cowpea/Alectra and TL II projects indicate that the overriding preference<br />
trait by farmers is seed size; large seeded are more liked than small seeded types.<br />
The backcrossing programme at ARI Ilonga will continue to backcross 6 by October 2010, while at<br />
Bunda College will be at back cross 4. There will be an urgent need to request for an extension of the<br />
project as the intended cowpea materials will have not reached the farmers at the end of the funding year<br />
2010.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />
Team Report<br />
Insights and lessons learned<br />
During the monitoring tour the team found that the activities planned for 2009 were underway. This was<br />
continuation of activities started in year 1 and 2, including breeding for Alectra resistance and on-farm<br />
evaluation of promising cowpea lines, market surveys, cowpea processing and utilisation as well as new<br />
work on cowpea macro and micro nutrient analysis in cowpea products. Also introduced to the project<br />
was the training of farmers on seed production i.e. production of Quality Declared Seeds (QDS).<br />
There was an outbreak of cutworms on the demonstrations planted in Singida and this gave a learning<br />
lesson that cowpea seed should be dressed with insecticide to prevent damage by cutworms especially at<br />
two leaf stage.<br />
Variability was observed in the reaction of individual cowpea lines to Alectra across sites. Seed was<br />
therefore provided from a range of locations in both countries for a glasshouse screening trial at Long<br />
Ashton UK. This confirmed variability in the host range of parasite samples from Malawi and Tanzania<br />
At a species level there are 3 strains of Alectra by host range: Alectra from Singida in Tanzania attaches<br />
and emerges on cowpea and groundnut but not on mung or common bean. Alectra from Bihawana and<br />
Ismani in Tanzania attaches and emerges on cowpea, groundnut, and common bean but not on<br />
mung bean. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and<br />
mung bean. Within cowpea, patterns of virulence are evident: Alectra from all sites in both countries<br />
emerges on IT36E-16 and TZA 263 but not on B301. There is need to confirm this by using<br />
biotechnology techniques.<br />
Three selected cowpea lines with traits farmers like, including IT99K-7-21-2-2-1, IT99K-753-1 and TZA<br />
267 are being increased under irrigation at Ilonga <strong>Research</strong> Station to be distributed to many farmers for<br />
wider evaluation in the 2009/2010 season<br />
Due to poor cowpea crop performance during this season, the intended market stakeholder’s meeting<br />
was postponed to next season in Tanzania but it was undertaken in Malawi and a report is being<br />
prepared.<br />
A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />
Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual Agricultural<br />
Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in AGREST conference<br />
series Vol. 8. (in press). The proceedings will be ready within and outside the country. Mr Gabriel<br />
Martin’s got financial support from the project for his MSc research work
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Cowpea Utilization in Iringa and Dodoma Regions<br />
Peter S. Mamiro 1 , A. M. Mbwaga 2<br />
Introduction<br />
Mature dry cowpeas are important in the diets of many population groups around the<br />
world. Africa alone accounts for 7.5 million hectares of the estimated world total area, of<br />
about 10 million hectares. Of the 7.5 million hectares, about 70% lies in West and Central<br />
Africa (Singh et al., 1996). This food legume is readily available, inexpensive and<br />
popular part of the traditional food system. Cowpeas (Vigna unguiculata L.Walp) along<br />
with other legumes are recognized as an important source of protein (Agazounon et al,<br />
2004). However, their contribution to the overall diet and dietary mineral needs is less<br />
well known. For instance nutritional deficiencies of iron and zinc are often widespread in<br />
developing countries, where staple diets are frequently plant-based and consumption of<br />
expensive meat products is low. Cowpea is an annual legume, cultivated dried fresh grain<br />
and long stored dried grain being cooked while others are dehulled to remove the seed<br />
testa, then ground to obtain flour. The flour is used to make various dishes or recipes. The<br />
young and tender cowpea leaves are picked and eaten as relish along with the main<br />
staples. The grains and leaves are the source of carbohydrates, proteins, fats, vitamin A<br />
(ß-carotene), B and C, which are necessary for maintaining good health and fighting<br />
disease. Young cowpea leaves are consumed in at least 18 countries in Africa, and seven<br />
countries in Asia and the Pacific (Ahenkora et al., 1998). Cowpeas are among the top<br />
three or four leaf vegetables marketed and consumed (Barret, 1990). Cowpea also<br />
contains iron, calcium, phosphorus, magnesium, copper, zinc and is high in dietary fibre<br />
(Sinha and Kawatra, 2005). The other vegetative parts of the cowpea plant after removal<br />
of the grain, are used as feed, forage, hay and silage for livestock. This paper reports on<br />
the extent of utilization of cowpeas and its contribution to the macro and micro nutrients<br />
to communities that cultivate and consume the legume.<br />
Methodology<br />
Survey<br />
A survey to determine the pattern of utilization of cowpea was conducted in Iringa and<br />
Dodoma region in Kalenga and Msinga villages respectively. The choice of these villages<br />
was undertaken in collaboration with government extension authorities from the two<br />
regions. The communities are well known for the production, selling and consumption of<br />
cowpeas. The two regions fall within two different agro ecological zones,. Iringa being a<br />
wet region located in the southern highlands zone and Dodoma being a semi arid region<br />
in the central zone. A simple structured questionnaire seeking information on the quantity<br />
and frequency of consumption of cowpeas and other foods at the homestead was<br />
1<br />
1.Department of Food Science and Technology, Sokoine University of Agriculture, P. O. Box 3006,<br />
Morogoro, Tanzania.<br />
2 Uyole Agricultural <strong>Research</strong> Institute, Box 400, Mbeya, Tanzania
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
administered to 511 randomly selected farmers. Samples of local cowpea varieties and<br />
products prepared from cowpeas were collected for analysis in a laboratory at Sokoine<br />
University of Agriculture. The processing and preparation steps of cowpea recipes and<br />
products were documented.<br />
Proximate analysis of the samples<br />
The food samples for chemical analysis were grounded to pass through a one millimeter<br />
screen in a Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (%<br />
DM) was determined by drying the sample in an oven at 103 ºC – 105 ºC for 24 hours.<br />
Crude protein percentage (% CP) was determined by Kjeldahl method AOAC method<br />
No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030 analyzer, whereby percentage<br />
Nitrogen obtained was used to calculate the % CP using the relationship: % CP = % N x<br />
6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />
System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and<br />
percentage ash (% minerals) was determined immediately after the dry matter<br />
determination by incinerating the samples in a muffle furnace at 550 ºC for four (4)<br />
hours. The ash was cooled in a desiccators and then weighed. Crude fiber percentage (%<br />
CF) was determined by using the fiber system and Weende method (AOAC 1995).<br />
Nitrogen free extract percentage (% NFE) was calculated by differences as shown: %<br />
NFE = 100 - (% moisture + % CP + % EE + % CF + % Ash).<br />
Total minerals<br />
Total mineral content of the samples was carried out after dry ash. The ash was dissolved<br />
in a solution of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture<br />
was 6NHCL. Total iron, zinc and calcium were determined by atomic absorption<br />
spectrophotometry by AOAC method 970.12 No. (AOAC, 1995).<br />
Data analysis<br />
Data for minerals and proximate analysis were entered in SPSS 12.0 for windows<br />
computer software. Descriptive statistics was used to determine the measures of central<br />
tendency for each analyzed cowpea variety. An analysis of variance of the results was<br />
done at 95% confidence interval (P≤0.05) using Tukeys Honestly Significant Difference.<br />
Homogeneity test was performed to determine homogenous sets.<br />
Results<br />
Demographic characteristics of farmers<br />
There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116<br />
(48%) were females. In Dodoma 277 farmers were interviewed, 135 (49%) were males<br />
and 142 (51%) were females (Table 1). Farmer’s age ranged between 18 to 83 years with<br />
a mean of 45±15 years while in Dodoma the range was between 20 and 59 years with a<br />
mean of 35±7 years. In the two regions, the majority of the farmers had only studied up<br />
to class 7 (57% in Iringa and 88% in Dodoma) which is primary school level. However,<br />
5% and 1% of interviewed farmers had no formal education in Iringa and
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up<br />
to six family members.<br />
Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />
Parameter Category Iringa Dodoma<br />
n % n %<br />
Gender Male 124 51.7 135 48.7<br />
Female 116 48.3 142 51.3<br />
Education Class: 3-7 164 70.4 257 92.8<br />
Class 8-14 58 24.2 16 5.9<br />
No education 13 5.4 4 1.4<br />
Household size 1-6 members 192 80.8 183 66.8<br />
7-14 members 48 19.2 94 33.2<br />
Cowpea harvested, procured and utilization<br />
Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own<br />
cowpea, or obtained cowpea supplies from friends and relatives. Few of the farmers<br />
purchased cowpea for home consumption (Table 2). Farmers harvested an average of 75<br />
kg of cowpeas per household with a range from 53 to 118 kg and the amount sold per<br />
household ranged from 4 to 73kg with an average of 25kg per household. The amount<br />
kept at home for consumption ranged from 36 to 97 kg with an average of 52 kg. A<br />
substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />
between 100 and 1000 grams of cowpea leaves for household consumption depending on<br />
the household size. Per capital consumption ranged from 40 to 200 gm of cowpea grain<br />
for 84% of households in Iringa and 94% in Dodoma. At the same time per capital<br />
consumption for cowpea leaves ranged from 10 to 500 grams by 95% of households in<br />
Iringa and 100% in Dodoma, respectively.<br />
On days when cowpeas are cooked in a household, the same relish will be eaten with<br />
either rice or stiff porridge during lunch, dinner and with breakfast the following<br />
morning. More than 50% of households in Iringa and Dodoma consumed cowpea at least<br />
once and up to three times a week (Table 2). Cowpea leaves were also prepared and<br />
eaten on separate days. The leaves are consumed fresh especially during wet season and<br />
in dried form during the lean or dry season. Almost all farmers in the two regions<br />
consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff<br />
porridge (ugali), a mixture of dehulled maize and cowpeas (kande) and most frequently<br />
the cowpea buns (bagia).
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Table 2: Farmers Responses on Cowpeas Utilization<br />
Parameter Category Iringa Dodoma<br />
n % n %<br />
Ever eaten cowpea Yes 240 100 277 100<br />
How often eaten per day Twice 92 38.5 156 56.3<br />
Thrice 147 61.5 121 47.7<br />
How often eaten per week Once 77 32.1 28 10.1<br />
Twice 75 31.3 119 43.0<br />
Thrice 65 27.1 82 29.6<br />
Four Times 11 4.6 38 13.7<br />
Five Times 12 5 10 3.6<br />
Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />
Purchase from the market 41 17.1 6 2.2<br />
Get from the neighbor 75 31.3 140 50.5<br />
How much cowpea is prepared in one meal 100-500gms 137 57.1 115 76.9<br />
500-2000gms 103 42.9 64 33.1<br />
How much cowpea leaves are prepared in one<br />
meal<br />
100-1000gms 146 61.9 227 89.7<br />
1000-4000gms 90 39.1 26 11.3<br />
Consumed green cowpea leaves Yes 240 100 277 100<br />
Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />
Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />
20 – 90 minutes 133 65.7 214 79.4<br />
Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />
20 – 90 minutes 31 54.4 72 62.1<br />
How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />
Wash-chop-cook 214 90.7 44 44<br />
Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />
Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />
Cowpea roasted 17 2.1 44 4.8<br />
Roasted milled to flour to prepare porridge Cowpea Porridge 28 3.5 66 7.2<br />
Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />
Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />
Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />
201-500 39 16.2 19 7.0<br />
Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />
500 - 1400 12 5.1 0 0
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Content<br />
Minerals of nutritional importance that were analyzed in cowpea products included iron,<br />
zinc and calcium. Analysis showed that all varieties had significantly different (p
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />
Moisture<br />
(%)<br />
Ash<br />
(%)<br />
Ca<br />
(mg/kg)<br />
Zn<br />
(mg/kg)<br />
Fe<br />
(mg/kg)<br />
Local Cowpea Dodoma 9.40 3.89 958.14 31.45 28.90<br />
Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />
Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />
Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />
Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />
Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />
Local Cowpea Iringa 9.56 3.66 992.43 32.56 27.61<br />
Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />
Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />
Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />
Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />
Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />
Can we explain the difference from the two sites<br />
Proximate composition<br />
Proximate analysis showed that the cowpea varieties with the highest crude protein<br />
content were IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%)<br />
Varieties with lowest iron included IT00K-1207 (22.01%) and TZA 263 (22.6%). For<br />
fats, IT97K499-8 (11.18%), IT99K-7212-2-1 (10.98%) and Fahari (10.33%) varieties had<br />
the highest levels while the lowest included IT97K819-118 (8.17%) and IT96D-733<br />
(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118<br />
(16.1%). The lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%)<br />
and VULI 2 (13.1%). Among the best new lines with regards to crude protein and fats<br />
content were IT97K499-8 and IT99K-7212-2-1.<br />
Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />
Variety/Food component % DM %CP %CF %EE %NFE<br />
VULI 1 89.25 24.02 13.77 9.12 38.18<br />
IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />
IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />
IT96D-733 88.54 23.44 14.91 8.34 37.68<br />
IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />
IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />
FAHARI 89.29 25.46 13.45 10.33 35.86<br />
TUMAINI 89.13 25.96 13.83 9.89 35.23<br />
IT00K-1207 87.95 22.01 15.66 8.46 37.82
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />
TZA263 89.21 22.60 17.21 8.86 36.45<br />
VULI 2 89.53 25.64 13.10 10.25 36.53<br />
IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />
B301 90.11 23.75 14.85 8.39 38.85<br />
IT99K-7212-2-1 89.68 26.00 12.53 10.98 35.89<br />
Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />
Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />
Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />
Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />
Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />
Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />
Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />
In comparison with the improved lines and varieties, local cowpeas have comparable<br />
levels of dry matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table<br />
5). However, the levels of fats are relatively lower (5.4%) in comparison with the<br />
improved lines/ varieties ranging from 8% to 11.2%. A similar trend is shown with<br />
cowpea leaves. Dehulling the cowpeas reduces crude fibre significantly with a gradual<br />
fall in fats. With regard to the cowpea buns from Iringa and Dodoma regions there was<br />
significant drop in crude protein and crude fibre but a very significant rise in fats<br />
(14.3%).<br />
Discussion<br />
Macro and micro nutrient content<br />
In the two surveyed regions it was revealed that cowpeas are produced by majority of the<br />
farmers and consumed to a relatively large extent by all respondents, which provides an<br />
indication of cowpea importance in the two communities. Similarly, the survey revealed<br />
that cowpeas are prepared and consumed in a number of ways, such as relish for stiff<br />
porridge and rice, mixed and boiled with dehulled or un-dehulled maize and cowpea<br />
buns. In both regions, the cowpea buns were important as they were also a source of<br />
income in a number of households. The buns however, appear to have lower iron and<br />
calcium, especially those from Iringa region. The calcium, zinc and iron content of the<br />
cowpea buns were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma<br />
region had comparatively higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and<br />
iron (16.3mg/kg). This difference might emanatre from the method of preparation of<br />
cowpeas in Iringa in comparison to Dodoma. In Iringa the grains are dehulled and soaked<br />
for about three hours and then washed in flowing river water for about an hour. This<br />
procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />
these minerals are largely lost during washing.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
With regard to the deep frying of the cowpea buns, though some nutrients such as<br />
proteins and crude fibre were significantly reduced (from average of 22% to 19%<br />
protein), in the process fats were significantly increased (from average of 5% to 14%),<br />
while nitrogen free extracts and dry matter remained stable. The oil increase is attributed<br />
to the sunflower oil used to deep fry the buns. The farmers also cultivate sunflower for<br />
home use and sale. Greater oil intake is better as daily activities require adequate energy<br />
supply.<br />
Analysis of the developed cowpea varieties showed relatively high protein and fat in the<br />
levels of 24- 26% and 8%-11% respectively. Similar results have been observed by<br />
Horax et al., (2004) where they found cowpea seed contained 20-25% protein and was<br />
rich in essential and non-essential amino acids. Asante et al. (2006) were investigating on<br />
32 accessions of cowpea seeds, found protein and tannin contents ranging from 16.4-<br />
27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006)<br />
proximate analysis of various infested cowpea samples indicated increases in protein and<br />
crude fiber contents and decreases in moisture, ash, fats/oil and carbohydrates contents<br />
when compared with those of the uninfected samples. Percentage increases in protein and<br />
fiber contents ranged between 9.31-30.56%, and 1.24-15.11%, respectively while<br />
percentage decreases were, moisture (3.97-10.23%); ash (3.75-12.87%); fats/oil (1.43-<br />
10.00%); and carbohydrate (1.88-7.50%).<br />
Similarly the developed cowpeas were relatively high in calcium, iron, and zinc.<br />
However, considerable mineral variation was found among the analyzed cowpeas<br />
varieties. Similar study by Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea<br />
variety with the highest calcium concentration of 2096.0 μg/g and Zn was detected in only few<br />
varieties ranging from 1501.0 μg/g to 2071.0 μg/g. Analysis of variance of the samples<br />
showed significant differences (p
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
With regard to cowpea leaves, it was observed that the leaves are consumed by almost all<br />
in the two communities surveyed. The young leaves are the ones normally picked,<br />
usually the first three or four from the cowpea plant. While some chop the leaves into<br />
small pieces before cooking, others boil the whole leaves. The leaves seem to have a big<br />
contribution with regard to calcium, iron and zinc. According to Schippers (1997)<br />
African Indigenous Vegetables (AIVs) play a highly significant role in food security of<br />
the under-privileged in both urban and rural settings. AIVs have traditionally taken an<br />
important role in African diets, being used both as medicine and as a vegetable. Many of<br />
these crops are highly nutritious, easy and cheap to grow.<br />
Results indicate that, there was twice as much calcium in leaves than in cowpea grains<br />
and almost 40 times as much iron. Similar results were observed by Weinberger et al.<br />
(2004) where indigenous vegetables showed high mineral levels. Since the iron and zinc<br />
levels in cowpea leaves have shown to be significantly higher than the levels in cowpea<br />
grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />
contribute to alleviating micronutrient deficiencies from cheap sources especially in<br />
resource-poor families. However, it is also important to note that, vegetables that are<br />
common in a particular locality do not necessarily have values of iron contents that are<br />
comparable. For example, in a study by Weinberger et al. (2004) it was reported that<br />
cowpea leaves were identified and collected from four districts in Tanzania, their values<br />
were quite different varying from 179.0 mg in Kongwa, 66.0 mg in Singida, 77.5 mg in<br />
Muheza and 187.0 mg in Arumeru, per kg of edible portion. This is evident that the<br />
amount of minerals found in these vegetables does differ not only according to type of<br />
vegetable but also according to the place, location or district where they were obtained.<br />
This shows that the soil mineral content might have an influence in mineral uptake of the<br />
plants. This aspect can be further investigated.<br />
Adequacy of cowpea consumed in households<br />
As revealed in the results, the daily per capita consumption for the majority of the<br />
households surveyed ranged from 41 to 200 grams of cowpea, which means using the<br />
Atwater factors (4 kcal/g carbohydrates, 4 kcal/g protein and 4 kcal/g fats) an individual<br />
obtained about 135 to 658 kcal per day. Considering that a normal adult requires about a<br />
supply of 2500 kcal per day to perform moderate activities, the other amount of food<br />
eaten in conjunction with cowpeas such as rice or maize flour cannot fill the remaining<br />
gap. This shows that the amount of cowpeas consumed by most households does not<br />
satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed<br />
varieties, which are higher yielding and resistant to Alectra, they will probably be<br />
accepted by the communities and the amount per capita of cowpeas consumed should<br />
increase provided they continue to cultivate the same area of land.
<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />
Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
Conclusion<br />
Cowpea grain and leaves are widely consumed in the two surveyed areas. The<br />
contribution of micro and macro nutrients is significant for both developed lines and local<br />
varieties but with leaves having greater mineral content than grain. Therefore awarenessraising<br />
within communities to consume more cowpea leaves is required. However the<br />
amount produced per household is low compared to requirements. Consequently cowpea<br />
intake per capita is low, and does not meet the RDA. However, since households in both<br />
areas still have ample land they should be encouraged to increase the area planted as well<br />
as planting higher yielding varieties in conjunction with preferred local varieties. This<br />
does however require further market investigation.<br />
Other important nutrients such as vitamins, amino acid profile and levels of<br />
antinutritional factors (tannins and phytates) should also be further investigated. The<br />
vitamins and amino acids influence in nutrition while the antinutritional factors influence<br />
on the bioavailability of the nutrients in the body.<br />
Acknowlegement<br />
The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong><br />
<strong>Foundation</strong>, who facilitated successful survey and laboratory analysis of macro and<br />
micronutrients.<br />
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Development and promotion of Alectra resistant cowpea cultivars for<br />
smallholder farmers in Malawi and Tanzania<br />
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