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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Edited by:<br />

A M Mbwaga ARI Uyole, Tanzania<br />

Joseph Hella Sokoine University of Agriculture, Tanzania<br />

J. Mligo Ilonga <strong>Research</strong> Institute, Tanzania<br />

V. Kabambe Bunda College University of Malawi<br />

September 2009<br />

1<br />

2009


Project Team<br />

<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Institution Name<br />

Tanzania<br />

Uyole Agricultural <strong>Research</strong> Institute, PO<br />

Box 400, Mbeya<br />

Ilonga Agricultural <strong>Research</strong> Institute, PO<br />

Box 33, Kilosa<br />

Sokoine University of Agriculture<br />

PO Box 3007, Morogoro<br />

Dodoma Rural District Council<br />

PO Box 1089, Dodoma<br />

Dr A M Mbwaga (Pathologist & Project Leader)<br />

~ Dr J K Mligo (Cowpea breeder)<br />

~ M. Mchomvu (Home Economics)<br />

~ Dr Joseph Hella (Agric. Economist)<br />

~ G. Martin (MSc Student)<br />

~ D. Muywanga (District Agricultural and Livestock<br />

Officer)<br />

~ Ms Mwamba (Subject Matter specialist)<br />

Singida Rural District Council ~ B. Manento (DALDO)<br />

~ L. Sakwera (<strong>Crop</strong>s specialist)<br />

Iringa Rural District Council<br />

PO Box 290<br />

INADES Formation, Tanzania<br />

PO Box 203, Dodoma<br />

Bunda College, University of Malawi, PO<br />

Box 219, Lilongwe<br />

Chitedze <strong>Research</strong> Station<br />

PO Box 158, Lilongwe<br />

Department of <strong>Crop</strong> Production, Ministry of<br />

Agriculture. PO Box 1035, Capital City,<br />

Lilongwe<br />

International Institute of Tropical<br />

Agriculture. C/O Lambourne Ltd, 26<br />

Dingwall Rd, Croydon CR9 3EE, UK<br />

~ P. Mphwewe (DALDO)<br />

~ Mrs Nyalusi (<strong>Crop</strong>s Specialist)<br />

A. Katunzi (Director & Training specialist)<br />

Malawi<br />

~ Dr V Kabambe (Weed Scientist, Senior Lecturer &<br />

project coordinator for Malawi)<br />

~ T Chilongo (Agricultural Economist)<br />

~ Dr J. Bokosi, (Plant Breeder)<br />

~ Dr A Mangwela (Food scientist)<br />

~ Ms Kazila. (MSc Student)<br />

~ William Henderson Harawa<br />

~ BSc Student in Agricultural Economics<br />

~ Mr E Mazuma (Pathologist & National Commodity Team<br />

Leader, Pulse <strong>Research</strong>)<br />

~ Ms C Mtambo. Entomologist and Chief <strong>Crop</strong> Protection<br />

Officer<br />

Nigeria<br />

Boukar Ousmane (Breeder) IITA Kano Nigeria<br />

2<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table of Contents<br />

Page<br />

Summary VI<br />

Introduction 2<br />

Project objectives 2<br />

Summary of research activities 2<br />

Objective 1. Development of high yielding A. vogelii resistant cowpea varieties 2<br />

Objective 2. Promotion of high yielding A. vogelii resistant cowpea varieties 13<br />

Implications of the research findings 15<br />

Annex 1 Tanzania Country Report 17<br />

Annex 2: Cowpea Utilization in Iringa and Dodoma Regions, Tanzania 23<br />

Annex3: Malawi Country Report 32<br />

3<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Summary:<br />

The parasitic weed Alectra vogelii is a wide spread constraint to cowpea production in semi-arid areas<br />

of Southern Africa, where the crop is an important source of protein for resource poor farmers. A<br />

project aiming to develop and promote Alectra resistant cowpea cultivars was initiated in Malawi and<br />

Tanzania. Farmer research groups have been established in cowpea growing areas of both countries<br />

and have been focusing on evaluation of cowpea lines for adaptation, yield, resistance to Alectra and<br />

processing and utilization of cowpea grain for three seasons now. Initial studies of farmers and market<br />

trader’s preferences in both countries Malawi and Tanzania indicate that large white to-cream seed<br />

types are most favored. Growers also need plant types that produce copious foliage for spinach and for<br />

preservation for consumption in the dry season. Cowpea germplasm collected from local institutions,<br />

farmers and the breeding program at the International Institute of Agriculture (IITA) Nigeria was<br />

evaluated. In Tanzania this season farmers have selected from five promising to three lines for their<br />

yield performance, Alectra resistance and earliness. Selected cowpea lines included IT99K-7-21-2-2-1,<br />

IT99K-573-1 and TZA 263. These lines are now being multiplied at Ilonga Agricultural <strong>Research</strong><br />

Institute for planting by more farmers on a large scale next season.In Malawi line IT99K-7-21-2-2-1<br />

was selected the best in terms of Alectra resistance, yield and grain size and is being earmarked for<br />

release. Parallel to this work some of these materials have been analyzed for macro and micro-nutrient<br />

content at Sokoine University of Agriculture Tanzania (see next section below). Post-graduate student<br />

projects on production, utilization and marketing of cowpea were completed in Tanzania last year, but<br />

there are still to be completed at Bunda College in Malawi.<br />

The processing of cowpea especially into grain was found to be a laborious work for women. Using<br />

tradition method of preparing - for example buns (Bagia) - a woman had to sleep late and to wake up<br />

around four in the morning ready to prepare Bagia. After introduction of new ways of preparing flour<br />

women are now it takes less than an hour to prepare Bagia, hence there is a lot of time saving. This can<br />

enable each member of the family to prepare Bagia for the children each morning. It has also been<br />

observed that dehuled grain can store for a long time without getting attacked by bruchids. The dehuled<br />

grain also cooks fast compared to grain. This is hoped to increased usage of cowpea grain at family<br />

level.<br />

Studies on micronutrients included also the frequency uptake of cowpea products by farmers in<br />

sampled villages of Iringa and Dodoma Tanzania Minerals of nutritional importance that were<br />

analyzed included iron, zinc and calcium; results indicated that, there was twice much calcium in leaves<br />

than in cowpea grains and almost 40 times as much iron. Since iron and zinc levels in cowpea leaves<br />

have shown to be significantly higher than the levels in cowpea grains, creating more awareness and<br />

encouraging the utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies<br />

from cheap sources especially from resource-poor families. The variety that was best with regards to<br />

all minerals was IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and<br />

fats content were IT97K499‐8 and IT99K‐721‐2‐2‐1.<br />

4<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction:<br />

Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural households in<br />

the drier and sub-humid region of Southern Africa, where diets tend to be overly reliant on starchy foods<br />

such as millet, sorghum, maize and cassava. The addition of even a small amount of cowpea ensures the<br />

nutritional balance of the diet and enhances the protein quality by the synergistic effect of high protein<br />

and high lysine from cowpea and high methionine and high energy from cereals. Hence cowpea grain is<br />

an inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />

particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />

harvest also it adds cash to the household after selling the grain and dried leaves. It has to be noted that<br />

cowpea is mainly a women crop.<br />

On-farm cowpea yields are extremely low, averaging 319 kg/ha in Tanzania and 388 kg/ha in Malawi.<br />

Use of late maturing cultivars, low plant density and insect damage are widely recognized as important<br />

constraints to improved cowpea production under on-farm conditions. Less well appreciated is the<br />

importance of the parasitic weed Alectra vogelii, which attaches itself to the roots of cowpea plants and<br />

interferes with the plants' ability to obtain water and nutrients. Recently-released improved cowpea<br />

cultivars that are earlier maturing and more tolerant to key insect pests and diseases are especially<br />

susceptible to Alectra attack, experiencing up to 50% yield reductions. A. vogelii is widespread from the<br />

Northern Province of South Africa, through Central Africa to Kenya and across West Africa to Mali. In<br />

Tanzania, A. vogelii is common in Mwanza, Shinyanga, Dodoma, Iringa, Mtwara and Ruvuma regions,<br />

while in Malawi, it is common in Lilongwe, Dowa and districts in central Malawi, the lower lying, drier<br />

areas of the southern region and the Blantyre/Shire Highlands<br />

Project objectives<br />

The general objective of the project is to improve cowpea productivity on A. vogelii-infested land in<br />

Malawi and Tanzania by introducing Alectra resistance into cowpea cultivars that are also early<br />

maturing, pest/disease tolerant and high yielding. The specific objectives are to develop high yielding A.<br />

vogelii resistant cowpea cultivars and then to promote them in both countries, Malawi and Tanzania.<br />

SUMMARY OF RESEARCH ACTIVITIES:<br />

Objective 1: Development of high yielding Alectra vogelii resistant cowpea varieties<br />

In year three of the project further studies have been undertaken to consolidate breeding through transfer<br />

of resistance to Alectra from the resistant lines (donor parents) into the commercial released varieties<br />

(recurrent parents) which are susceptible to Alectra by using a backcross method to improve the<br />

released varieties both in Malawi and Tanzania. Farmers groups established continued to be<br />

strengthened through training and participation in joint activities both within and outside their villages.<br />

Farmers in study villages continued to conduct on farm evaluation of the promising cowpea lines with<br />

commercially released varieties as checks. This year, 10 promising lines in Tanzania and five lines in<br />

Malawi were evaluated by farmers based on their own suitability criterion. In year three, project team<br />

continued to develop partnerships with farmer groups thus allowing continued on farm trials and<br />

availing useful data to research for making informed decisions in on-station breeding programmes.<br />

Through exchange visits and joint design of cowpea breeding programme, research collaboration<br />

between Malawi and Tanzania was increased and capacity was enhanced through farmer group training<br />

and graduate (BSc. and MSc.) students training. Details of activities undertaken on the outputs that the<br />

project had planned to address during the 2008/09 season (year 3) are presented in the individual county<br />

reports in the Annexes. A highlight of this work is as follows<br />

Output 1.1: Producer and consumer preference in cowpea identified<br />

The activities under this output were completed in year 1 and 2 and are found in annual report of 2008.<br />

5<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />

In Tanzania farmer groups have expanded from 6 to 8 and emphasis of the training was on the new<br />

farmer groups. Similarly two farmer groups were added in Malawi. The training given to the new farmer<br />

groups was on group formation, leadership and writing a group constitution. Training was also<br />

conducted on seed production using demonstration plots.<br />

Participatory Video shooting was one of the new activities introduced to farmers this season starting in<br />

Tanzania. Two farmer groups from Kikombo village (12farmers) in Dodoma and Mangalali village (14<br />

farmers) in Iringa region participated in the participatory video shooting training course. , The outcome<br />

has shown to be very effective in empowering farmers to document activities they are undertaking in the<br />

project. Their participation at CoP3 in Bagamoyo Tanzania was very evident and has created a<br />

motivation for them to undertake more of these documentation of project activities.<br />

Output 1.3: Cowpea lines resistant to A vogelii identified<br />

Assembling of cowpea germplasm:<br />

The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />

obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />

Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />

starting in the same year one. In Malawi the germplasm used came from IITA and from their national<br />

collection and Chitedzi <strong>Research</strong> Institute.<br />

On-station screening of cowpea cultivars for Alectra resistance<br />

In Tanzania, this activity started in 2007 growing season and the promising lines were tested to more<br />

Alectra infested sites. This year the trials were planted at three on-station locations which included<br />

Ismani, Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those<br />

from last season showing promising Alectra resistance and farmers had already selected some of them<br />

as the best lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288,<br />

TZA 263, IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207 and B301. VULI-2 and FAHARI<br />

were included as local checks. The materials were planted at a plot size of 3m x 4m, 4 rows per plot and<br />

replicated four times. Data recorded included; plant stand count at harvest, Alectra count per plot and<br />

yield per plot (kg/plot). Yield data was computed to yield in kg per ha.<br />

Generally weather condition was not very good for the crop, because rains started late and were erratic.<br />

Dodoma suffered the most for all the crops and this affected the crop establishment and Alectra<br />

infestation. From the test cowpea materials, only cowpea line IT97K-499-38 and B301 supported zero<br />

Alectra counts at three locations. (Table1). Other lines supported low Alectra plants as compared to the<br />

susceptible checks (Vuli-2 and Fahari) or TZA 263, and IT99K-573-2-1.The susceptible varieties<br />

(released commercial varieties) are being improved by incorporating resistance to Alectra by backcross<br />

method. At present the work is at backcross three progressing into backcross four, this will be followed<br />

by selfing then screening for resistance to Alectra.<br />

Table 1: Average infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />

locations at 12 weeks after planting 2009:<br />

Cowpea Entries Ismani Bihawana Hombolo Mean Comments<br />

FC<br />

Alectra<br />

count/sites<br />

IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of<br />

Alectra<br />

IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of<br />

Alectra<br />

IT97K-499-8 0.0 0.5 0.0 0.2<br />

IT97K-818-35 0.0 1.0 0.0 0.3<br />

6<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

IT97K-819-118 0.0 4.5 0.0 1.5<br />

IT97K-499-38 0.0 0.0 0.0 0.0<br />

IT89KD-288 0.0 26.3 0.0 8.8<br />

IT00K-1207 0.8 1.8 0.0 0.9<br />

IT96D-733 0.0 42.0 0.0 14.0<br />

B301 0.0 0.0 0.0 0.0<br />

TZA 263 0.0 1.5 0.0 0.5 Farmers selected<br />

for large grain<br />

Vuli 2 3.0 48.3 0.3 17.2 Commercial<br />

released varieties<br />

Fahari 7.5 34.3 1.0 14.3 Commercial<br />

released varieties<br />

Mean 0.87 12.73 0.15 7.58<br />

SE 0.41 2.98 -<br />

Range: 0-16 0-74 0-3<br />

In Malawi five of entries selected for further testing based on Alectra resistance, farmer preferences on<br />

grain characteristics and according to evaluations conducted in the last two years were planted at<br />

Chitedze and Bunda College. The trial’ design was complete block design, with 4 replicates. The trial<br />

plots had 5 rows, 4 m long and 0.75 m apart. Other traits evaluated included: grain yields and yield<br />

components (e.g. seed per pod,), days to flowering and maturity and disease incidences.<br />

Screening for Alectra resistance, entries IT 97K-825-15; IT99K-7-21-2-2-1 and IT 99K-494-6 recorded<br />

least number of Alectra counts (Table 2). Entries IT99K-494-6 (1369kg/ha), IT82E-16 (1786kg/ha) and<br />

Sudan 1(1827kg/ha) recorded high yield and the same entries showed larger number of seeds per pod<br />

than other entries<br />

Table 2: Alectra counts m -2 taken after flowering at all the trial sites in Malawi<br />

Entrycode & name Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />

IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />

IT97K-825-15 0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />

T99K-7-21-2-2-1 0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />

IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />

Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />

IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />

Farmers’ local 2.80 1.14 3.29 - 0.03 0.11 1.23<br />

Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot.<br />

With assistance from Village Extension Officers farmers were able to collect data and evaluate the<br />

materials based on their own set criterion.<br />

As discussed earlier, rainfall distribution was a major problem. It started late and erratic; hence there<br />

was very poor crop establishment especially in Iringa and Dodoma region. Singida received well<br />

distributed rainfall but there was a serious outbreak of cut worms on legumes in that region. The Alectra<br />

infestation was only reported on commercially released varieties and local cultivars, the other lines were<br />

observed free from the parasitic weed. The average highest cowpea yield was recorded from Singida<br />

region followed by Iringa and last Dodoma as presented in Table 3.<br />

Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania<br />

2009<br />

Cowpea line Farmer group managed to harvest from their plots of cowpea<br />

Mkungugu Iringa Kikombo Dodoma Msungua Singida<br />

IT99K-7-21-2-2-1 600 222 600<br />

IT99K 573-2-1 720 440 600<br />

IT97K 818-35 200 389 800<br />

IT89KD 288 400 333 200<br />

TZA 263 280 167 1200<br />

VULI-2 560 - 600<br />

FAHARI 800 - 760<br />

Village Local- 200 - 530<br />

Mean 470.0 310.2 661.3<br />

On-farm evaluation of cow pea lines (responses from farmers)<br />

Results of the on farm evaluation of selected cultivars was conducted in five participating villages;<br />

Msungua and IKhanoda (Singida region), Kikombo (Dodoma region), Mkungugu and Mangalali (Iringa<br />

region) based on the criterion they identified during the 2008 growing season i.e. resistance to Alectra,<br />

diseases/insects/pests, high yielding, large white to cream coloured seed, early maturity, production of<br />

many and tender leaves, drought resistance. These criteria were matched with five lines presented in<br />

Table 4.<br />

Table 4: Five best lines selected in 2008 evaluation session<br />

Lines Line description based on 2008 selection<br />

IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding, white<br />

coloured seed,<br />

IT 99K 573-1-1 Slightly late maturing, resistant to Alectra, average seed size, good seed colour,<br />

high yielding<br />

1T 97K 818-35 Late maturity, resistant to Alectra<br />

1T 89KD 288 Late maturity, high yielding, resistant to Alectra, normal seed colour,<br />

TZA 263 Late maturity, resistant to Alectra, good colour, large seeded<br />

Results indicated that line TZA263, followed by IT 99 K-7-21-2-2-1 and IT 99K-573-1-1 suited<br />

farmers’ social, economic and cultural interest. Based on these findings, lines TZA 263, IT 99K-7-21-2-<br />

2-1 and IT 99K-573-1-1 are to be increased for distribution to many farmers for further evaluation in<br />

2010 season. Initial process to establish descriptors for these lines for possible inclusion in the National<br />

Performance Trials (NPT) towards variety releases will be initiated.<br />

On farm evaluation in Malawi was conducted in Riviri, Bunda College, Mngwangwa, Mpokwa<br />

Khuvinda village, Mpokwa Chibisa village, and Lisasadzi. Seven entries involving four lines IT98K-<br />

8<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

503-1, IT97K-825-15, IT99K-7-21-2-2-1, IT99K-494-6, and two released varieties Sudan -1, IT82E-16<br />

and one farmers’ local cultivar were evaluated against five main criteria, which are; seed size, plant<br />

type, early maturity, insect resistance and disease resistance. Note that varieties Sudan -1 and IT82E-16<br />

were included for counterchecking with introduced lines. Preliminary description of the lines is<br />

presented in Table 5.<br />

Table 5: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />

Code Name Description<br />

5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra vogelii<br />

resistance<br />

37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />

preference<br />

9 IT99K-7-21-2-2- Best Alectra resistance in ’07, ‘07/08, good seed size, medium seed size,<br />

1<br />

good yield<br />

21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium seed size,<br />

low farmer preference<br />

53 Sudan -1 Released variety, good resistance to Alectra and good yielder in ‘07/08<br />

52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08. Poor<br />

resistance to A. vogelii. Well liked by farmers.<br />

54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />

Results of analysis show preference on the following order. Sudan 1 is most preferred followed by<br />

IT82E-16, IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6. Lines IT97K-825-15 followed by<br />

farmers’ local are less preferred as indicated above. Since Sudan 1 and IT82E-16 are already released,<br />

varieties IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6 may be considered for further genetic<br />

perfection on-station with a possibility of releasing them commercially starting with line IT99K-7-21-2-<br />

2-1.<br />

Geographic variability in host response to A. vogelii<br />

Studies undertaken in pot experiment at Long Ashton UK indicate that at a species level there<br />

are 3 strains of Alectra by host range:<br />

1. Alectra from Singida attaches and emerges on cowpea and groundnut but not on mung or common<br />

bean.<br />

2. Alectra from Bihawana and probably Ismani attaches and emerges on cowpea, groundnut, common<br />

bean but not on mung bean<br />

3. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and mung<br />

bean.<br />

Within cowpea, patterns of virulence were also observed to be evident: Alectra from all sites in both<br />

countries emerges on IT36E-16 and TZA 263 but not on B301.<br />

1. Alectra from Bunda and Kasungu emerges on ITK1207, IT97K 818-35, IT97K819-118 and IT<br />

99K7-21-2-2-1<br />

2. Alectra from Zomba emerges on ITK1207, IT97K 818-35, IT97K819-118 but not on IT 99K7-21-<br />

2-2-1 (this result for IT 99K7-21-2-2-1 same as in 2008 pot trial).<br />

3. Alectra from Singida and Ismani does not emerge on ITK1207, IT97K 818-35, IT97K819-118 and<br />

IT 99K7-21-2-2-1 (result for Singida same as in 2008 trial)<br />

4. Alectra from Bihawana emerges on IT 99K7-21-2-2-1 but not on ITK1207, IT97K 818-35 and<br />

IT97K819-118.<br />

In Tanzania it appears that Alectra from Singida has a narrower species host range and less virulent on<br />

the cowpea lines tested than Alectra from other sites. Bihawana Alectra provides a "universal" test for<br />

9<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

the three Tanzania sites. From this study the cowpeas that are resistant at Bihawana are also resistant at<br />

Singida and Ismani.<br />

In Malawi it appears that Alectra from Bunda and Kasungu are similar but different to Zomba.<br />

It has to be also noted that the Alectra samples we have from Malawi are different from the parasite<br />

collected further south near Blantyre as in trials some time back at Long Ashton this attacked B301<br />

(Mainjeni, 1999).<br />

It has been more confusing to find the result for IT81D-994 as this was resistant to all samples in 2008.<br />

There must be something went wrong with the samples delivered to Long Ashton this year.<br />

The most reliable method for characterization of these strains would be to go for using molecular<br />

markers.<br />

Reference:<br />

Mainjeni, C. E. (1999) The host range of Alectra vogelii Benth. From Malawi and resistance in common<br />

bean and cowpea. Msc. Thesis, University of Bath, UK. pp 83.<br />

Table 6: Response of 11 cowpea, one mung bean, one bambara groundnut and two groundnut lines to<br />

five accessions of A.vogelii.<br />

Alectra accession<br />

Zomba Malawi (ex cowpea) Bunda Malawi (ex groundnut) Kasungu Malawi (ex groundnut)<br />

Line Emerged No of<br />

reps<br />

Cowpea<br />

Un‐<br />

emerged<br />

Emerged No. of<br />

reps<br />

10<br />

Un‐<br />

emerged<br />

Emerged No. of<br />

reps<br />

2009<br />

Un‐<br />

emerged<br />

IT86E‐16 19.3 + 9.7 3 3 19.3 + 17.5 3 3 20.3 + 4.6 3 3<br />

B301 0 0 2 0 0 3 0 2 0 1<br />

TZA‐263 19 + 8.7 3 3 14.7 + 7.3 3 3 8.3 + 5.9 3 3<br />

IT00K‐1207 2.3 + 1.9 2 3 9 + 2.1 3 3 5.7 + 3.2 2 3<br />

IT81D‐994 1.3 + 0.7 2 3 1.3 + 0.9 2 3 0.3 + 0.3 1 3<br />

IT97K‐818‐35 1.3 + 1.3 1 3 2.7 + 1.8 2 3 3.3 + 2.8 2 2<br />

IT97K‐819‐118 0.7 + 0.7 1 3 3.3 + 2.4 2 3 1 + 0.6 2 2<br />

IT99K‐7‐21‐2‐2‐<br />

1<br />

Common bean<br />

0 0 3 0.7 + 0.2 2 3 0.7 + 0.3 2 3<br />

Kabalabala 1.7 + 1.7 1 NR 0 0 NR 2.7 + 2.2 2 NR<br />

Maluwa 4.3 + 2.2 2 3 3 + 0.8 3 3 4 + 2.5 3 3<br />

Masaka 1.0 + 0.6 2 3 5 + 4 2 3 3 + 1.7 2 3<br />

Mung bean<br />

Ex Tanzania 1.7 + 1.7 1 2 4 + 0.8 3 3 2.0 + 0.6 2 3<br />

Groundnut<br />

Chalimbana 0.7 + 0.7 1 NR 0.3 + 0.3 1 NR 1.3 + 1.3 1 NR<br />

CG7 2 0.5 + 0.4 1 NR 1.7 + 0.9 2 NR 2 + 1 2 NR<br />

1 Only one replicate pot established. 2 Only two replicate pots established, NR = not recorded yet.<br />

Table 6 continued.<br />

Singida Tanzania (ex cowpea)<br />

Alectra accession<br />

Bihawana Tanzania (ex cowpea) Ismani Tanzania (cowpea)<br />

Line Emerged No of Un‐ Emerged No. of Un‐ Emerged No. of Un‐<br />

Cowpea<br />

reps emerged<br />

reps emerged<br />

reps emerged<br />

IT86E‐16 13.3 +<br />

3.5<br />

3 3 9.3 + 6.8 3 3 3 + 0 3 3


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

B301 0 0 0 0 0 0 0 0 0<br />

TZA‐263 3.3 + 1.5 3 3 0.7 + 0.3 2 3 0.3 + 0.3 1 2<br />

IT00K‐1207 0 0 0 0 0 0 0 0 0<br />

IT81D‐994 0 0 1 2 2<br />

1 1 0.3 + 0.3 1<br />

IT97K‐818‐<br />

35<br />

0 0 2 0 0 1 0 0 1<br />

IT97K‐819‐<br />

118<br />

0 0 1 0 0 2 0 0 0<br />

IT99K‐7‐21‐<br />

2‐2‐1<br />

Common<br />

bean<br />

0 0 2<br />

0.7 + 0.3<br />

2<br />

2 0 0 1<br />

Kabalabala 0 0 NR 0 0 NR 0 0 NR<br />

Maluwa 0 0 2 1.3 + 0.3 3 3 0.3 + 0.3 1 2<br />

Masaka<br />

Mung bean<br />

0 0 3 2 + 1 3 3 0.3 + 0.3 1 2<br />

Ex Tanzania<br />

Groundnut<br />

0 0 0 0 0 0 0 0 0<br />

Chalimbana 0.7 + 0.7 1 NR 0 0 NR 0 0 NR<br />

CG7 0 0 NR 0 0 NR 0 0 NR<br />

NB: Mean number emerged stems per pot (+ S.E.) at 97 days after sowing. For entries with no emerged<br />

A. vogelii, the number of replicate pots with unemerged parasite attachments is shown. No standard<br />

errors are shown when there were less than three pots per cowpea/A. vogelii combination.<br />

Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />

Project activities are leading to increased capacity in cowpea research in Malawi and Tanzania and the<br />

strengthening of collaboration between institutions in both countries. Within the project, two researchers<br />

Abubakar Mzanda from Tanzania and Dr Vernon Kabambe from Malawi participated in a week long<br />

statistics training course offered by <strong>McKnight</strong> <strong>Foundation</strong> at Centre for Agricultural <strong>Research</strong><br />

Development (CARD)Bunda College University of Malawi June 15-22, 2009.<br />

• Two project staff – Drs J.P. Hella and V Kabamabe attended ASARECA review workshop on<br />

climate change. The workshop was held at Naivasha Kenya.<br />

• A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />

Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual<br />

Agricultural Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in<br />

AGREST conference series Vol. 8. (in press). The proceedings will be ready within and outside<br />

the country. Mr Gabriel Martin’s got financial support from the project for his MSc research work<br />

• Group dynamics training was conducted for all the farmers in the two participating villages in<br />

Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />

leadership and operation. This group strengthening included membership and responsibilities,<br />

constitution, networking and fund raising to sustain the groups.<br />

• During the season an undergraduate student (Ms Elida Kazira )undertook a study on cowpea time<br />

of planting x variety x density trial titled - Farmer Participatory Selection of Alectra Resistant<br />

Cowpea Varieties in Selected Districts of Malawi). The student has submitted her thesis for<br />

examination.<br />

• Participatory video workshop was held at INADES Dodoma, where two extension staff from the<br />

projects sites Dodoma and Iringa respectively participated and these also went back and trained<br />

farmers from the groups at Mangalali Iringa (14 farmers) and Kikombo in Dodoma (12 farmers)<br />

11<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 1.5: Project Monitored and evaluated<br />

To accomplish Activity 1.5.2 (Review progress against M & E criteria each year) monitoring and<br />

evaluation activities were conducted in Tanzania and Malawi as planned. The objective was to evaluate<br />

on going on-farm and on station cowpea project activities and crossing work in screen house.<br />

The monitoring and evaluation for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro)<br />

from 27 th to 30 th April, 2009 with participants including Ambonesigwe Mbwaga (Project Leader) ARI,<br />

Uyole Mbeya Tanzania, Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania; Vernon Kabambe (Weed<br />

specialist) Bunda College, Lilongwe, Malawi; James Bokosi (Breeder) Bunda College, Lilongwe,<br />

Malawi; Boukar Ousmane (Breeder,) IITA Nigeria. On-farm visits were done in regions of Iringa and<br />

Dodoma, whereas on-station visits were done at Ismani (Iringa region), Bihawana (Dodoma region and<br />

at Ilonga research institute (Morogoro region). Crossing for cowpea varieties to improve resistance to<br />

Alectra is being conducted.<br />

In Malawi, monitoring and evaluation was conducted from 15 th to 20 th March 2009. The team was<br />

composed of Drs Joseph Mligo (cowpea breeder), Ambonesigwe Mbwaga (Project team leader), Charlie<br />

Riches (CoP Liaison Officer) and Malawian counterparts comprising Dr Vernon Kabambe (Country<br />

project leader), Mosses Mamiliro (Breeder, and James Bokosi (Breeder). For On –farm activities 7<br />

cowpea cultivars of which 5 were experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-<br />

2-2-1, IT 97K 825-15, Bunda 1, IT98K503-1) one commercially released variety (IT 82E-16) were<br />

monitored in Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu district in<br />

Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA, Also seed<br />

multiplication of experimental cowpea lines such as TZA 263 (from Tanzania) and IT 81D-994. Seed<br />

increase also included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and<br />

IT82E-16.<br />

For on-station activities, the team visited Bunda college experimental site where seed multiplication of<br />

experimental cowpea lines which have shown Alectra resistance was being conducted. The<br />

performances of the materials were good and were at flowering stage in addition to seed multiplication<br />

activities at Bunda.<br />

Overall M&E team observed the following<br />

• Weather variability (especially drought) is an overriding problem for cowpea production. The<br />

team recommended the collaboration with the Tropical Legumes II project of IITA and try to<br />

incorporate both traits (Alectra resistance and drought tolerance) in the current breeding<br />

programme.<br />

• Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least<br />

preferred by the farmers. At all sites farmer’s variety was the latest, and it had not even<br />

flowered when all the other entries were maturing.<br />

• It was noted that only early maturing varieties were most preferred, forgetting the advantage of<br />

late maturing varieties for not having other traits such as prolonged availability of leaves as<br />

relish. This suggests that there is a need to evaluate the materials according to maturity groups.<br />

• The good production practices should be incorporated in the screening of the varieties against<br />

Alectra. Hence, there is need to expose the groups involved in the project to all these production<br />

practices.<br />

• There is a need to evaluate the materials according to maturity group: early, medium and late<br />

because farmers’ selections are dictated and influenced by time to maturity<br />

• The backcrossing programme at Ilonga will continue to backcross 6 by October 2010, while at<br />

Bunda will be at back cross 4. Request for extension of the project should be initiated early<br />

2010<br />

Overall the team noted good progress which are being made and high enthusiasm among stakeholders<br />

especially farmers who are conducting on-farm demonstrations<br />

12<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Objective 2: Promotion of high yielding A. vogelii resistant cowpea varieties<br />

With the continuation of the on-farm trials/demonstration component of the project efforts to increase<br />

awareness of the problem of A. vogelii and approaches to management continued in year 3. Work<br />

implemented included farmers field exchange visits, participation in national agricultural shows, training<br />

on preparation of different cowpea recipes and some market studies in Malawi<br />

Output 2.1: Farmer and extension awareness of .A. vogelii and use of resistant cultivars enhanced<br />

In the implementation of the above mentioned output, two activities were conducted in Tanzania, these are<br />

farmer field days and exchange visits and participation in 2009 National Agricultural show. With respect to<br />

exchange visit, twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu (Fahari research<br />

group) and later both visited Ismani On-station trials in Iringa. The objective of the farmer exchange visit<br />

was for farmers from Mangalali to share experiences with other farmers on principles of cowpea<br />

production, processing and utilization and to be acquainted with on station cowpea lines screening work. At<br />

the research station farmers participated in evaluation of 14 cowpea screening materials planted. Four<br />

cowpea farmers of the CCRP project from Singida rural district (two from Msungua and two from<br />

Ikhanoda) were sponsored by their district council and attended the National Agricultural Show between 1 st<br />

and 8 th August 2009 in Dodoma. Promising cowpea lines and associate processed products of cowpea from<br />

their group were displayed.<br />

In Malawi, farmers from Mngwangwa EPA, on their own accord, organized themselves and participated at<br />

a District Agriculture Fair held in the same EPA in June 2009. The farmers demonstrated a range of<br />

cowpea products based on the training made by the project and shared this with participants. In addition,<br />

the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science Department. Again,<br />

the focus of the exhibit was on utilization. The exhibits attracted a lot of attention from participants<br />

Output 2.2a: Nutritional status of farmers improved<br />

To accomplish output 2.2 above, activity 2.2.2: (Develop/modify/ demonstrate cowpea products) was<br />

undertaken. The primary objective was to promote the utilization of cowpea in Tanzania. Specifically was<br />

to train farmers on the processing of cowpea grains into dhal, preparation of cowpea dishes (snack bites<br />

from cowpea flour “bagia’); and to perform sensory evaluation and organoleptic tests. The training was<br />

conducted in eight villages namely Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />

Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The villages were<br />

purposively selected as a result of their involvement in a project on “Improvement of cowpea productivity<br />

on Alectra Vogelii infested land in Tanzania. A one day workshop was conducted in each project village<br />

and farmers were trained on improved cowpea technologies. The trainings were based on demonstrations,<br />

hands-on trials and printed materials (brochures for cowpea recipes). Farmers participated in the validation<br />

of five recipes for cowpea value addition. Sensory and organoleptic tests were carried out, where by<br />

farmers were required to rank and find out the most preferred types of ”bagia”. The five types of bagia<br />

were made using different recipes. The recipes were translated into Swahili language for better<br />

understanding by farmers.<br />

Table 7: Cowpea snack product recipes<br />

Type of<br />

Materials/ingredients<br />

Bagia<br />

1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />

2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />

3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and water<br />

4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and water<br />

5. Bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs, yeast and<br />

water<br />

13<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

In Malawi where farmers already have advanced knowledge on preparation of different<br />

cowpea/groundnut recipes, in areas where cowpea seed was provided, farmers were encouraged to use<br />

their cowpea to practice the different recipes which they learned from the project<br />

B: Cowpea Utilization in Iringa and Dodoma Regions<br />

Per capital consumption of 40‐200gms of cowpea grains were found to be 84% in Iringa and 94% in<br />

Dodoma, while per capital consumption for cowpea leaves (10‐500 grams) were 95% in Iringa and<br />

100% in Dodoma, respectively. More than 50% of households in Iringa and Dodoma consumed cowpea<br />

once to three times a week. The leaves are consumed fresh especially during wet season and in dried<br />

form during lean/dry season. Almost all farmers in the two regions consume cowpea leaves<br />

Macro‐ and micro‐ element analysis<br />

Analysis of the improved cowpea cultivars showed relatively higher protein levels of 24% to 26% and<br />

8% to 11% fat content. Similarly the improved cowpeas were relatively high in calcium, iron, and zinc.<br />

However, considerable mineral variation was found among the analyzed cowpeas varieties.<br />

Surprisingly, local cowpea cultivar had comparable high mineral calcium, zinc and iron concentrations<br />

compared with the improved varieties. This shows that apart from other merits that the improved<br />

cowpeas might have over local varieties such as higher yielding and resistance to certain diseases and<br />

pests, still nutritionally they are equally good. (See Annex 2)<br />

Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />

seed identified<br />

The seed availability is a big issue because commercial companies are not interested in producing seeds<br />

of low volume crops like sorghum, pearl millet bambara nuts and cowpea. To accomplish Activity 2.3.2<br />

{Train farmers to produce seed (QDS) for commercial use}, farmers were given basic training in<br />

community seed production covering four main topics namely Organization of seed production in<br />

Tanzania (L 1), Attributes of seed quality (L 2), Cowpea Seed Production (L 3) touching base on issues<br />

related to site selection for cowpea seed production, land preparation, choice of varieties to be planted<br />

according to days to maturity, and plant spacing depending on variety growth habit, crop husbandry<br />

practices such as pest management, rouging of off types, harvesting, seed conditioning, seed storage and<br />

seed sampling and testing for quality attributes. Table 8 present numbers of participants to on-farm seed<br />

production<br />

Table 8:. Participants to on-farm seed production for project participating farmers<br />

Region District Village Nane of<br />

No. of Topics Covered<br />

<strong>Research</strong> Group participants L1 L2 L3<br />

SINGIDA Singida Msungua Mshikamano 10 X X X<br />

rural Ikhanoda Mfwenji 20 X X X<br />

Nduu Matumaini 21 X X X<br />

DODOMA Dodoma Kikombo Ushirika 11 X X<br />

rural Mpunguzi Twende na<br />

wakati<br />

10 X X<br />

IRINGA Iringa Mkungugu Ari mpya 10 X X<br />

rural Mangalali Fahari 14 X X<br />

In Malawi, the project provided small packs of seed of 0.5 kg each to all project participating farmers at<br />

Mpokwa, Mngwangwa EPA (about 60 farmers at each EPA) and Kasungu. The objective was to create<br />

awareness on cowpea production. Subsequently, a follow-up training was conducted on pest<br />

management, to grow cowpea intensively (as sole crop) and to practice pest management and observe<br />

14<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

results. Farmers were monitored and they managed to get good crops and yields and were motivated to<br />

produce cowpeas. These farmers have been advised to store some of their seeds so that they can plant<br />

more cowpeas next season. They were also trained on principles of cowpea seed production during field<br />

days and visits.<br />

Output 2.4: Cowpea marketing opportunity identified<br />

In Malawi, cowpea market value chain workshops which brought representatives of cowpea producers,<br />

middlemen and traders were conducted in Lilongwe and Zomba in August 2009. The workshops were<br />

held to establish the linkages between cowpea producers (farmers) with the markets and to identify<br />

increased marketing opportunities. The workshop revealed that there are six big business traders who<br />

buy cowpea seeds (ADMARC, Rab Processors, Mulli Brothers, Agora, Grain Traders Association, and<br />

Transglobe) most of them paced emphasis on large seeded grain types as preferences.<br />

In Tanzania, the workshop was not held due to poor cowpea yields as a result of poor rainfall; hence<br />

farmers had nothing to offer to the traders. It is scheduled for May 2009 in Dodoma<br />

A paper - Gabriel, M. M.; Hella, J.P. and Mbwaga, A.M (2008) Assessment of Cowpea Marketing<br />

Efficiency: A case study of selected regions in Tanzania” will appear in the forthcoming AGREST<br />

conference proceedings Vol. 8 which is in press. Plan is underway to publish a paper in an international<br />

Journal for wider circulation. This is the first paper on cowpea marketing published in Tanzania<br />

Implication of the research findings<br />

During the past year, the project partners have built on work begun in year 1 and 2 to strengthen farmer<br />

groups, establish on farm trials and demonstrations, to take forward a cowpea-breeding programme,<br />

cowpea marketing stakeholders and to finalize marketing studies for Malawi. Crossing programme to<br />

incorporate resistance to Alectra into released commercial varieties has reached backcross 3 going in<br />

backcross 4 in Tanzania and backcross 2 in Malawi (due to delayed start in Malawi) These<br />

achievements will allow the project to move forward with next stage of the agreed work plan to<br />

undertake activities in the following ways;<br />

1. The project continues to support capacity building of farmer groups especially those new ones so<br />

that they are well informed about the aims of the project. Providing short term benefits for group<br />

membership should encourage continued participation in trials and other project activities.<br />

2. Crossing programme will continue up to backcross 6 by November 2010 in Tanzania, while in<br />

Malawi will be at backcross 4 because of late start.<br />

3. Wide scaling out identified Alectra resistant cowpea lines for Tanzania are three lines<br />

TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 and in Malawi are entries IT97K-825-15,<br />

IT99K-494-6 and IT99K-7-21-2-2-1<br />

4. Market studies have been completed in Tanzania and a stakeholders meeting will be conducted next<br />

financial year and in Malawi data is being analysed, a separate report will be prepared. Preliminary<br />

results were shared during the value chain workshops held in Lilongwe and Zomba respectively<br />

5. Farmer groups continued to be equipped with knowledge on QDS production and eventually. It is<br />

envisaged that QDS seed be bought by the project and then re-packed and farmers be given each<br />

member of the group give five new farmers of his choice to produce grain which can then be<br />

marketed by the group with aim to increase volume of grain production and hence increase grain for<br />

market at the same time popularize the varieties to more farmers.<br />

6. Since iron and zinc levels in cowpea leaves have shown to be significantly higher than the levels in<br />

cowpea grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />

15<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

contribute in alleviating micronutrient deficiencies from cheap sources especially for resource-poor<br />

families.<br />

7. The new line, which was best with regards to all minerals, was IT99K-7-21-2-2-1 while the best<br />

with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1. The later has<br />

been selected by farmers for its earliness in both countries Tanzania and Malawi (Item 3 above). It<br />

will be one of the potential candidate to be promoted for release in both countries Malawi and<br />

Tanzania<br />

16<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 1: Tanzania Annual Report<br />

Development and promotion of Alectra resistant cowpea cultivars for smallholder farmers in<br />

Malawi and Tanzania<br />

Edited by:<br />

Dr Ambonesigwe M Mbwaga: Uyole Agricultural <strong>Research</strong> Institute, Box 400 Mbeya<br />

Dr Joseph Mligo: Ilonga Agricultural <strong>Research</strong> Institute, Box 33 Kilosa, Morogoro<br />

Dr Joseph Hella: Sokoine University of Agriculture, Morogoro<br />

September 2009<br />

1<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Tanzania Project Team<br />

Uyole Agricultural <strong>Research</strong> Institute Dr A M Mbwaga (Pathologist & Project Leader)<br />

PO Box 400, Mbeya<br />

Ilonga Agricultural <strong>Research</strong> Institute Dr J K Mligo (Cowpea breeder)<br />

PO Box 33, Kilosa<br />

Dodoma Rural District Council<br />

D Muywanga (District Agricultural and Livestock<br />

PO Box 1089, Dodoma<br />

Officer)<br />

Ms Stella Mwamba (Subject Matter specialist)<br />

Singida Rural District Council N Mosha (DALDO)<br />

L Sakwera (<strong>Crop</strong>s specialist)<br />

Iringa Rural District Council<br />

P Mphwewe (<strong>Crop</strong> specialist)<br />

PO Box 290<br />

INADES Formation, Tanzania<br />

A. Katunzi (Director & Training specialist)<br />

PO Box 203, Dodoma<br />

TOSCI P.O BOX 1056, Morogoro Mwakitwange (Seed Technologist)<br />

Photo: Cowpea Seed production plot at Mpunguzi village in Dodoma, Tanzania 2009<br />

2<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction:<br />

The rainfall amount and distribution during the 2008/09 season was poor in most dry areas of Tanzania.<br />

Also there was an outbreak of cut warms at seedling stage especially in Singida, where farmers had to<br />

re-plant legumes more than twice until they ran out of seed. This has led to poor harvest of the crop. The<br />

two outputs 1.1 and 1.2 mentioned below were covered in year one and year 2.<br />

Output 1.1: Producer and consumer preferences for traits in cowpea identified<br />

This out put has been completed in year two<br />

Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />

Three new more farmer groups were formed this season, one from each district of Singida, Dodoma and<br />

Iringa. These are from Nduu village (21 members) in Singida, Mpunguzi (10 members) in Dodoma and<br />

Ilambilole (8 members) in Iringa. Some preliminary training has been undertaken, mainly group<br />

formation, seed production and processing and utilization of cowpea.<br />

Output1.3.0: Cowpea lines resistant to A. vogelii identified<br />

1.3.1: To assemble cowpea germplasm:<br />

The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />

obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />

Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />

starting in the same year one.<br />

1.3.2 On-station screening of cowpea cultivars for Alectra resistance<br />

Introduction: Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural<br />

households in the drier and sub-humid regions of Eastern and Southern Africa, where diets tend to be<br />

overly reliant on starchy foods such as millet, sorghum, maize and cassava. Cowpea grain is an<br />

inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />

particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />

harvest, also it adds cash to the household after selling the grain, dried leaves and cookies like bagia<br />

(buns). Farmers grow local long duration cowpea cultivars, which produce low yield due to a number of<br />

reasons including Alectra infestation. In odder to improve the productivity of cowpeas in these areas,<br />

there was a need to introduce cowpea cultivars, which are high yielding, resistant to pests including<br />

Alectra and preferred by consumers both for market and for household use. The introduction of<br />

accessions was to asses for their resistance to Alectra. This activity started by this project in 2006/2007<br />

growing season and the promising lines were tested to more Alectra infested sites. These sites were<br />

Ismani in Iringa region; Bihawana Farmer Training Centre and Hombolo <strong>Research</strong> station both in<br />

Dodoma. For the second season 2007/2008 the cowpea lines were again planted at the same sites of<br />

Ismani, Bihawana FC and Hombolo to confirm their performance in terms of Alectra resistance and<br />

yield. Promising lines for Alectra resistance and yield were identified. In the third season 2008/2009, the<br />

promising lines were planted again at these Alectra hot spots for further confirmation of resistance to<br />

Alectra and for yield performance.<br />

Material and methods: The trial was planted at three on station locations which included Ismani,<br />

Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those from last<br />

season showing promising Alectra resistance and farmers had already selected some of them as the best<br />

lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288, TZA 263,<br />

IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207, B301 and VULI-2, FAHARI as local<br />

checks. The materials were planted at a plot size of 3x4m, 4 rows per plot and replicated four times.<br />

Data recorded were plant stand count at harvest, Alectra count per plot and yield per plot (g/plot). Yield<br />

data was computed to yield in kg per ha.<br />

3<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Results and discussions: Generally weather condition was not very good for the crop, because rains<br />

started late and erratic. Dodoma suffered the most for all the crops and this affected the crop<br />

establishment and Alectra infestation. The trial at Hombolo was mistakenly planted at a site where<br />

almost there was no Alectra infestation, only few Alectra plants were observed on susceptible cowpea<br />

checks Vuli-2 and Fahari. Looking at the susceptible checks high infestation of Alectra was observed at<br />

Bihawana Training Centre followed by Ismani. From the test cowpea materials only cowpea line<br />

IT97K-499-38 and B301 supported zero Alectra counts at both<br />

locations Ismani and Bihawana (Table 3.1). Other lines supported very low Alectra plants as compared<br />

to the susceptible checks Vuli-2 and Fahari.<br />

4<br />

2009<br />

Fig. 1 Cowpea field highly infested by Alectra at<br />

Bihawana Farmers training Centre Dodoma,<br />

2009<br />

Cowpea yield on average was observed highest at Hombolo followed by Bihawana FC and last was at<br />

Ismani. From the test cowpea lines, line IT99K-573-2-1 produced the highest average yield<br />

(735.6kg/ha) across sites followed by line IT97K-819-118 (647.3kg/ha). These lines also gave the<br />

highest yield from the test cowpea lines in 2008 season (Project Annual report 2008). Test line TZA 263<br />

was severely infected with a bacterial disease called bacterial blight especially at Ismani and Hombolo;<br />

in some of the plots almost all plants died due to the disease<br />

Fig. 2 Cowpea line TZA 263 attacked by a bacterial<br />

disease likely to be Bacterial blight (Xanthomonas<br />

vignicola)


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 1.Average Infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />

locations at 12 weeks after planting 2009:<br />

Cowpea Entries Ismani Bihawana<br />

FC<br />

Hombolo Mean Alectra<br />

count across<br />

5<br />

sites<br />

Comments<br />

IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of Alectra<br />

IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of Alectra<br />

IT97K-499-8 0.0 0.5 0.0 0.2<br />

IT97K-818-35 0.0 1.0 0.0 0.3<br />

IT97K-819-118 0.0 4.5 0.0 1.5<br />

IT97K-499-38 0.0 0.0 0.0 0.0<br />

IT89KD-288 0.0 26.3 0.0 8.8<br />

IT00K-1207 0.8 1.8 0.0 0.9<br />

IT96D-733 0.0 42.0 0.0 14.0<br />

B301 0.0 0.0 0.0 0.0<br />

TZA 263 0.0 1.5 0.0 0.5 Farmers selected for large<br />

grain<br />

Vuli 2 3.0 48.3 0.3 17.2 Commercial released varieties<br />

Fahari 7.5 34.3 1.0 14.3 Commercial released varieties<br />

Mean 0.87 12.73 0.15<br />

SE 0.41 2.98 -<br />

Range: 0-16 0-74 0-3<br />

Table 2: Yield (kg/ha) of cowpea entries at Alectra vogelii hot spot at three locations, 2009<br />

Cowpea Entries Ismani Bihawana Hombolo mean yield Comments<br />

FC<br />

across sites<br />

IT99K-7-21-2-2 372.8 527.1 568.7 489.6 Farmers selected for<br />

earliness<br />

IT99K-573-2-1 309.4 927.1 970.4 735.6 Farmers selected for yield<br />

IT97K-499-8 410.2 643.7 753.9 602.6<br />

IT97K-818-35 432.3 525.0 608.2 521.8<br />

IT97K-819-118 590.8 677.1 674.0 647.3<br />

IT97K-499-38 362.5 409.6 604.2 458.8<br />

IT89KD-288 413.9 639.6 773.5 609.0<br />

IT00K-1207 262.9 545.8 515.2 441.3<br />

IT96D-733 404.6 302.1 514.3 407.0<br />

B301 164.8 952.1 789.2 635.4<br />

TZA 263 265.6 525.0 386.6 392.4 Large grain but severely<br />

infected by Bacterial blight<br />

disease.<br />

Vuli 2 212.7 354.1 867.3 478.0<br />

Fahari 209.8 554.2 745.6 503.2<br />

Mean 331.70 583.24 674.68<br />

SE 29.44 35.15 27.86<br />

Range: 92.5- 108.3- 236.6-<br />

791.7 1542.0 1198.0<br />

1.3.3a: On-farm evaluation of cowpea lines for Alectra resistance and yield:<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction: Most farmers are growing long duration trailing type of cowpea, and mostly around their<br />

homestead. They are mainly for getting leaves and few pods. The grain yields are usually very low due<br />

to low yield potentials of the cultivars and farmers do not spray against insect pests at flowering and<br />

podding, hence, most of the pods are lost. In addition, the local varieties are very susceptible to Alectra<br />

vogelii. The introduction of early, Alectra resistant and high yielding cowpea varieties will improve the<br />

nutritional status and increase income to the households by selling extra produced grain. There is high<br />

potential market for cowpea and for improving household nutritional status.<br />

Material and Methods: Due to shortage of cowpea seeds, five villages were selected for evaluation of<br />

five best selected cowpea lines from last season for Alectra resistance, adaptation and yield.<br />

Commercially released varieties Vuli-2 and Fahari were added and their village local checks. The<br />

villages involved were Mangalali and Mkungugu (Iringa region), Kikombo in Dodoma and other two<br />

villages were Msungua and Ikhanoda in Singida rural district.<br />

The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot,<br />

which were separated by one meter. Farmers wanted to have larger plots, but we were limited with<br />

amount of seed. With their chairman and secretary of the group and with assistance from extension<br />

officers farmers were able to collect data and evaluate the materials according to their criterion<br />

Results and discussion: As discussed under section 3.1 rains distribution was a major problem. It was<br />

late and erratic; hence there was very poor crop establishment such that many of farmers just managed<br />

to get few cowpea pods especially in Dodoma and Iringa sites. The other problem observed in Singida<br />

was for unforeseen outbreak of cut worms, which occurred at germination of the crop. Some farmers<br />

had to replant more than two times until they ran out of seed. In Singida rainfall started on time and it<br />

was relatively evenly distributed as compared to Dodoma and Iringa rural districts. The Alectra<br />

infestation was only reported on commercial and local cultivars, the other lines were observed free from<br />

the weed pest<br />

The yields recorded on few farmers who managed to get a crop are as presented in Table 3.. The<br />

average highest cowpea yield obtained was from Singida rural followed by Iringa and last Dodoma rural<br />

district.<br />

Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania 2009<br />

Cowpea line Farmer groups ir<br />

Mkungugu Kikombo Msungua<br />

Iringa Dodoma Singida<br />

IT99K-7-21-2-2-1 600 222 600<br />

IT99K 573-2-1 720 440 600<br />

IT97K 818-35 200 389 800<br />

IT89KD 288 400 333 200<br />

TZA 263 280 167 1200<br />

VULI-2 560 - 600<br />

FAHARI 800 - 760<br />

Village Local 200 - 530<br />

Mean 470.0 310.2 661.3<br />

1.3.3b: Farmer Participatory Cowpea Lines Evaluation<br />

Introductions: Cowpea (Vigna unguiculata (L) Walp), is one of the African indigenous most important<br />

leguminous crops. It is mostly grown in tropical countries for various uses and is a cheap source of<br />

vegetable protein. The crop is well adapted to relatively dry and stressful growing conditions of the<br />

marginal land, but produces excellent nutritive value (Singh et al., 1997). It is a drought tolerant crop<br />

and recently, there has been an increasing demand of cowpeas in the human diet, as well as for income<br />

generation. It thus provides solution for periodic hunger in semi-arid region of Tanzania<br />

6<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

This is the second season that the on farm evaluation of most promising lines of cowpea has been<br />

conducted in five villages namely; Msungua and Ikhanoda (Singida region), Kikombo (Dodoma region)<br />

and Mangalali and Mkungugu (Iringa region). Building from the 2008 evaluation, objectives of the 2009<br />

evaluation were as follow:<br />

~ To revisit the 2008 results<br />

~ To revisit evaluation criteria used in 2008<br />

~ To evaluate cowpea lines planted in 2009<br />

~ To make informed conclusion to guide for the subsequent on farm evaluation<br />

This brief presents the outcome evaluation of farmers’ trials in five villages in the above mentioned<br />

districts<br />

Method: The evaluation mission comprised Dr A.M. Mbwaga (Pathologist), Dr J.K. Mligo (Legume<br />

breeder) and Dr J.P. Hella (Agricultural Economist). As in 2008, the team participated in the on-farm<br />

evaluation in all villages for a period between 17 th and 24 th May 2009. In 2008 evaluation involved<br />

traversing with farmers in all five villages in each plot planted with a known variety of cowpea. Farmers<br />

were then requested to list, based on their own experience, the criteria which govern them to value and<br />

prefer particular varieties than another variety. Although main criteria varied across villages, but mostly<br />

hanged on high yield, early maturity, resistant to pests including Alectra, big sized seed and white to<br />

cream colour.<br />

Observations<br />

Criteria for selecting cowpea cultivar by villages in 2008<br />

Table 4 present the criterion used by farmers to rank different cowpea lines. The results were almost the<br />

similar in all villages. Good and more preferred lines are those associated with high yielding, early<br />

maturity, pest/disease (including Alectra) and drought resistant, large and white/cream seed colour.<br />

Table 4: Criteria for selecting different cowpea lines by village<br />

Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />

Early maturity High yielding High yielding High yielding High yielding<br />

Resistance to Alectra, Early maturity Drought Large seeds Large seeds<br />

diseases/insects/pests<br />

resistance<br />

High yielding varieties<br />

with many leaves<br />

Cream color Early maturing Early maturity Early maturing<br />

Brown color Alectra Resistant to Tolerant to Alectra<br />

resistant Alectra Alectra tolerance<br />

Big size- seeds Big size seed Cream Seed Reddish seed Drought<br />

color<br />

colour<br />

resistance<br />

Large seeds Plenty green<br />

leaves<br />

Cowpea lines preferred by farmers in target villages 2008<br />

Based on criteria highlighted in Table 4 above, in May 2008, farmers evaluated 14 lines including the<br />

commercial released varieties i.e. Vuli-2 and Fahari and locally grown cultivars named by the respective<br />

village name. List of cowpea lines evaluated in 2008 is presented in Table 5 below:<br />

Table 5: List of cowpeas line planted and evaluated in 2008 season<br />

No Lines/cultivar<br />

1 IT 99K-7-21-2-2-1<br />

2 IT 99K-573-1-1<br />

3 IT 97K-499-8<br />

4 IT 97K-818-35<br />

5 IT 97K-819-118<br />

6 IT 97K-499-38<br />

7<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

7 IT 89KD-288<br />

8 IT 00K-1207<br />

9 IT96D-733<br />

10 B 301<br />

11 TZA 263<br />

12 Vuli-2<br />

13 Fahari<br />

14 Farmer’s variety<br />

(Msungua/Ikhanoda/Kikombo/Makungugu/Mangalali)<br />

Table 6: Cowpea lines selected by each participating farmer Group in 2008 based on criteria in<br />

Table 4<br />

# Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />

1 IT99K-7-21-2-2 TZ A263 IT 00K-1207 TZA 263 IT 99K-7-21-2-2<br />

2 IT97K-499-8 IT99K-7-21-2-2 IT97K-818-35 IT 99K-573-1-1 IT 97K-499-8<br />

3 IT99K-573-1-1 IT97K-818-35 IT97K-499-38 IT 97K-499-8 IT 97K-819-118<br />

4 IT00K-1207 IT97K-499-38 IT96D-733 IT 89KD-288 TZA 263<br />

5 IT97K-818-35 IT99K-573-1-1 B301, IT 97K-499-38 IT 99K-573-1-1<br />

6 IT97K-499-38 IT97K-499-8 T99K-573-1-1, IT 97K-818-35 IT 97K-499-38<br />

Ranking for 2009<br />

At the beginning of the season, five out of 14 lines planted in 2008 were selected for planting at<br />

farmers’ plots. The lines selected for the current year was based in farmers’ own expression as indicated<br />

in Table 6 above. Lines presented for further evaluation and reasons advanced by farmers are as<br />

presented in table 7 below<br />

Table 7: Cowpea lines selected for on-farm experiment in 2009<br />

# Lines Varieties<br />

1 IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding,<br />

white cream coloured seed,<br />

2 IT 99K 573-1 Slightly late maturity, resistant to Alectra, average seed size, white<br />

cream seed colour, high yielding<br />

3 1T 97K 818-35 Late maturity, resistant to Alectra<br />

4 1T 89KD 288 Late maturity, high yielding, resistant to Alectra, white cream seed<br />

colour,<br />

5 TZA 263 Late maturity, resistant to Alectra, reddish brown colour, large seeded<br />

Msungua -Village, Singida<br />

In Msungua village, 16 farmers participated at the on-farm evaluation. Results are as presented in Table<br />

8 below. Line IT 99K 7-21-2-2-1 was the most preferred and IT89KD288 was the least preferred line.<br />

Table 8: Pair-wise ranking for Msungua village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 1 1 1 1 4 1<br />

IT 99K 573-1-1 2 2 2 3 2<br />

1T 89KD 288 4 5 0 5<br />

1T 97K 818-35 5 1 4<br />

TZA 263 2 3<br />

8<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Ikhanoda-Village, Singida<br />

At Ikhanoda village, 12 farmers participated at the on-farm evaluation and results are as presented in<br />

Table 9 below. Line TZA 263 was the most preferred while IT 89KD 288 was the least preferred line.<br />

Table 9: Pair-wise ranking for Ikhanoda village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2 1 1 1 5 3 2<br />

IT 99K 573-1-1 2 2 5 2 3<br />

1T 97K 818-35 4 5 1 4<br />

1T 89KD 288 5 0 5<br />

TZA 263 4 1<br />

Kikombo village, Dodoma<br />

In Kikombo village, 6 farmers participated at the on-farm evaluation and results are as presented in<br />

Table 10 below. Line TZA 263 was the most preferred but IT 89KD 288 was the least preferred line.<br />

Table 10: Pair-wise ranking for Kikombo village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 3 2<br />

IT 99K 573-1 1 2 3<br />

1T 97K 818-35 1 2 1 4<br />

1T 89KD 288 1 2 3 0 5<br />

TZA 263 5 5 5 5 4 1<br />

Mkungugu –Ismani, Iringa<br />

In Mkungugu village, 10 farmers belonging to Ari Mpya farmer research group participated at the onfarm<br />

evaluation. Results are as presented in Table 11 below. Line TZA 263 was the most preferred and<br />

IT 97K 818-35 was the least preferred line.<br />

Table 11: Pair-wise ranking for Mkungugu village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 1 1 1 5 3 2<br />

IT 99K 573-1 2 2 5 2 3<br />

1T 97K 818-35 4 5 0 5<br />

1T 89KD 288 5 1 4<br />

TZA 263<br />

Mangalali - Iringa<br />

5 1<br />

For unforeseen reasons it was not possible to evaluate the materials on day the team visited Mangalali<br />

village. When the farmers from Managalali visited Ismani research station, they were given opportunity<br />

to evaluate replicated trial planted with 14 lines including those planted at their own plots at Mangalali.<br />

The results are as presented in Table 12 below, where TZA 263 was ranked the best line followed by<br />

IT99K-7-21-2-2.<br />

Table 12: Ranking of the cowpea lines by Mangalali farmers at Ismani research Station 2009<br />

No Cowpea line Number of Number of Ranking of the Comment<br />

participants farmers selected cowpea line<br />

1 TZA-263 26 24 1 Each farmer<br />

2 IT99K-7-21-2-2-1 20 2<br />

was given a<br />

3 B 301 9 3<br />

chance of<br />

4 ITOOK-1207 8 4<br />

selecting only<br />

5<br />

6<br />

IT96D-733<br />

IT89KD-288<br />

8<br />

6<br />

4<br />

5<br />

best three<br />

cowpea lines<br />

7 IT99K-573-1 3 6<br />

9<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Conclusion<br />

The main objective of the field visitation was to evaluate farmer managed experiments in all study<br />

villages. Based on the approach and criteria agreed by the farmers, final rank on 5 lines introduced to<br />

them is presented in Table 10. The most preferred line is TZA, 263, followed by IT 99 K-7-21-2-2-1<br />

and IT 99K-573-1-1. Position four and five were contested between line IT 89KD-288 and IT 97K 818<br />

which were scored by two villages in the same positions. After revisiting some inherent characteristics<br />

of the lines as scored by same farmers in 2008, line IT 89KD-288 was relatively batter on same traits<br />

such as early maturity and leave sizes which are lacking in IT 97K-818. Thus IT 89KD-288 was ranked<br />

4 th while IT 97K-818 was ranked 5 th . as indicated in Table 10<br />

Table 10: Farmer preference with respect to 5 introduced cowpea LINES<br />

Villages Rank in order of preference<br />

1 st 2 nd 3 rd 4 th 5 th<br />

Msungua IT 99K 7-21-2-2-1 IT 99K 573-1-1 TZA 263 1T 97K 818-35 IT 89KD 288<br />

Ikanoda TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />

Kikombo TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />

Mkungugu TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 89KD 288 IT 97K 818-35<br />

Mangalali TZA 263 IT99K-7-21-2-2-1 1T89KD-288 IT99K-573-1 1T 97K 818-35<br />

Rank (1) TZA 263<br />

(2)<br />

IT 99K 7-21-2-2-1<br />

(3)<br />

IT 99K 573-1-1<br />

(4) IT 89KD 288<br />

(5) IT 97K 818-35<br />

Based on these findings, lines TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 are to be increased for<br />

distribution to many farmers for more evaluation. For these lines, initial process to establish descriptors<br />

for these lines for possible inclusion in the National Performance Trials (NPT) towards variety release<br />

should be started.<br />

1.3.4 Targeted crosses<br />

Tanzania cowpea crossing program<br />

In 2007 report 130 cowpea accessions were screened for Alectra resistance. 10 accessions could not<br />

support emergence of Alectra. The identified accessions that could not support emergence of Alectra<br />

plants were classified as resistant to Alectra. These were crossed to the desirable parents (the released<br />

varieties which are: Fahari, Tumaini, Vuli-1 and Vuli-2) with the aim of transferring the Alectra<br />

resistance into these released varieties. Since resistance to Alectra is conditioned by a single gene, a<br />

backcross method was used to transfer of resistance. At present the screening (September, 2009) is at<br />

backcross three plants. The resistant plants will be identified while they are still flowering, thus the<br />

resistant backcross three plants will be backcrossed to the desirable parents to obtain backcross four<br />

seed which will be planted and selfed then followed by screening for resistance to Alectra. This process<br />

will continue up to backcross six at which it is expected a single chromosome segment carrying the<br />

desired gene will have been incorporated into the desired parents. Planting of backcross four seed will<br />

be conducted in early November 2009. The selfed backcross four seed will be planted in late February<br />

2010 for screening again for resistance to Alectra and resistant plants backcrossed to the desired parent<br />

to produce backcross five seed. Backcross five seed will be planted for selfing in early May 2010 and<br />

the selfed backcross five seed will be planted for screening for Alectra resistance in early august 2010.<br />

Resistant plants will be backcrossed to desirable parent in October 2010 to produce backcross six seed.<br />

Backcross six seed will be selfed ready for evaluation for Alectra resistance and yield on Alectra<br />

infested fields in season 20010/011 with the expectation that the project will be extended.<br />

10<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 14: Backcross three crosses in the screen house at ARI-Ilonga by September 2009<br />

Cross number Pedigree<br />

17 (B301 x Vuli-1) x Vuli-1<br />

30 (IT 97K-499-8 x Vuli-2) x Vuli-2<br />

38 (Tumaini x IT96D-733) x Tumaini<br />

48 (ITK-499-8 x Tumaini) x Tumaini<br />

Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />

Activity 1.4.1: Across site evaluation (PL to visit programme in Malawi)<br />

A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra project activities<br />

The team was composite of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />

leader and Charlie Riches, CoP liaison officer and met by the Malawian project team that was<br />

composite of Dr Vernon Kabambe country project leader, Mosses Mamiliro (breeder) ,and James<br />

Bokasi (breeder)<br />

The evaluation tour for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro) from 27 th<br />

to 30 th April, 2009. The tour involved visiting seed multiplication, on-farm, on-station, and crossing<br />

programme. The team comprised of the following:<br />

Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />

Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />

Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />

James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />

Boukar Ousmane (Breeder,) IITA Nigeria<br />

Activity 1.4.2: Student projects<br />

For Tanzania an MSC student working on a project “Assessment of cowpea marketing efficiency; A<br />

case of selected regions of Tanzania” was completed and the student graduated November 2008.<br />

In Malawi an MSc student working on a project “Farmer Participatory Selection of Alectra Resistant<br />

Cowpea Varieties in Selected Districts of Malawi”. The thesis has been submitted to examiners for<br />

review. In addition in Malawi an BSc student is undertaking a study on “Cowpea variety x time of<br />

planting x density”<br />

Activity 1.4.3: Paricipation in CCRP "community of practie<br />

Three researchers, one extensionist and one farmer from the project attended CoP2 in Maputo<br />

Mozambique in October 2008. In CoP3 in Bagamoyo Tanzania 4 researchers and 4farmers from project<br />

sites attended<br />

Activity 1.4.4: End of project stakeholder’s workshop (location to be determined later)<br />

Output 1.5: Project monitored and evaluated<br />

Activity 1.5.1. Establish project level M & E criteria<br />

(done during partners meeting in Mbeya at the inception of the project)<br />

Activity1.5.2. Review progress against M & E criteria each year<br />

Introduction: A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra<br />

project activities.<br />

The team was composed of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />

leader and Charlie Riches( CoP- liaison officer) and met by the Malawian project team that was<br />

composed of Dr Vernon Kabambe country project leader, Mosses Mamiliro( breeder), and James<br />

Bokosi (breeder).<br />

The objective of the visit were<br />

To evaluate on going on-farm cowpea activities, on station project activities and crossing work in screen<br />

house<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

On –farm activities: On farm activity included evaluation of 7 cowpea cultivars of which 5 were<br />

experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-2-2-1, IT 97K 825-15, Bunda 1,<br />

IT98K503-1) one commercially released variety (IT 82E-16) and a farmer’s variety at each project site.<br />

The project sites included Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu<br />

district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA.<br />

Lilongwe district<br />

Ngwangwa Extension Planning Area (EPA)<br />

a. Mazila village: A farmer research group in Ngwangwa was composited of 30 members but due to<br />

shortage of cowpea seed only four farmers planted the 7 cultivars. The general performance of the<br />

cultivars was very poor which resulted in poor pod setting. The possible reasons are: the soil fertility<br />

looked very poor, also it appeared to have had high insect incidences.<br />

The Alectra infestation was very high especially on the released variety (IT 82E-16) and Bunda 1. Less<br />

infestation was observed on IT97K-825-15, IT99K-494-6 and IT99K-7-21-2-2-1.<br />

b. Mpondela: Here the group was able to visit field of one farmer. There was good performance of all<br />

the entries planted and there was no Alectra observed in all of the seven entries.<br />

Kasungu District<br />

Lisasi EPA<br />

Sikondeyani village: Five farmers planted the trial. The trial performance was very good compared to<br />

those of Ngwangwa EPA. This may be due to good soils and relatively good rain Although nearby fields<br />

planted with cowpea had very high Alectra infestation but the infestation levels in the trials was<br />

relatively low and late emergency for this reason the grain yield was not much affected. Farmers<br />

selected IT82E-16 and Bunda 1 as the best varieties in terms of yield and earliness.<br />

Zomba district<br />

Mpokwa EPA<br />

Khuvinda village: There was good performance of the cowpea lines with very low Alectra infestation.<br />

Out of the seven entries, two entries (IT82E-16 and Bunda 1) were selected by farmers to be the best<br />

due to their earliness and good pod setting. Although the released variety IT82E-16 is a short duration<br />

variety, farmers were not aware of such type of the variety. This emphasizes the need to carry out more<br />

demonstrations to create awareness to more farmers.<br />

Balaka district:<br />

Rivirivi EPA: This is the only area where farmers were not organized in a formal farmer research group.<br />

The demonstrations were very good and planted along the road side for everybody to see. Alectra<br />

infestation was very low. As in other villages, here farmers also selected the early maturing varieties as<br />

the best cultivars.<br />

Bunda College: Activities here included, seed multiplication of experimental cowpea lines which have<br />

shown Alectra resistance. These included TZA 263 (from Tanzania) and IT 81D-994. Seed increase also<br />

included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and IT82E-16. The<br />

performances of the materials were good and were at flowering stage. The materials for crossing were<br />

planted in screen house and were at vegetative stage. Some of them had supported Alectra emergence<br />

already, which were recommended to be removed out of the experiment because they had shown<br />

susceptibility to Alectra.<br />

Lessons learnt:<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least preferred by<br />

the farmers. At all sites farmer’s variety was the latest, and it had not even flowered when all the other<br />

entries were maturing.<br />

It was noted that only early maturing varieties were most preferred, forgetting the advantage of late<br />

maturing varieties for having other traits such as prolonged availability of leaves as relish. This suggests<br />

that there is a need to evaluate the materials according to maturity groups.<br />

Cowpea and pigeon pea as the main source of vegetable protein in the project target sites; it was<br />

observed that early maturity of the cowpea bridge the hunger period of February/March, while waiting<br />

for the pigeon pea to mature late in the season.<br />

Wrap up:<br />

- Released varieties showed better yields than test lines<br />

- Early maturing varieties save as source of food in hunger periods<br />

- Seed system for cowpea is very poor<br />

- More cowpea seed should be sent to Balaka and Kasungu districts as there is high demand for seed<br />

of the improved varieties.<br />

- Farmers in Rivirivi recommended forming farmer research groups for better access by researchers<br />

and extension staff as compared to individual isolated farmers<br />

TANZANIA<br />

27 th -30 th April, 2009<br />

Introduction: The monitoring and evaluation tour for Tanzania was done in three regions (Iringa,<br />

Dodoma, and Morogoro) from 27 th to 30 th April, 2009. The tour involved visiting seed multiplication,<br />

on-farm, on-station, and crossing activities. The objective was to monitor progress in implementing<br />

agreed strategies and action plans for the project. This report gives information on sites/farmers/stations<br />

visited, varieties evaluated, team observations and way forward.<br />

The monitoring and evaluation team comprised the following:<br />

Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />

Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />

Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />

James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />

Boukar Ousmane (Breeder,) IITA Nigeria<br />

On-farm visits: A number of farmers groups were visited in the two regions (Iringa, and Dodoma). In<br />

general farmers were required to plant five IITA lines and two locally released varieties (Fahari and<br />

Vuli-2). The two released varieties showed to be more adapted and performed consistently well across<br />

locations despite the prevalence of drought in many locations. The major problem is that they are both<br />

susceptible to Alectra. In a participatory variety trial conducted at Mpunguzi, Dodoma region, farmers<br />

selected IT 00K-126-3 as the best entry despite being susceptible to Alectra. Many of the farmers<br />

visited showed dedication to running the on-farm trials.<br />

On-station visits: The first on-station trial visited was at Ismani. The trial had 14 entries replicated four<br />

times. Each plot had five rows of four meter length. Although drought had an impact the performance<br />

of the entries was far better than the farmers’ fields. Alectra pressure was not as high as the previous<br />

year. This could have been due to the prolonged dry spell during the growing season. Vuli-2 and Fahari<br />

performed very well despite sustaining some Alectra plants. Except for the two check entries the rest<br />

had no Alectra emergence. The second trial was at Bihawana (Bihawana farmers training centre). The<br />

entries were similar to those planted at Ismani but four extra entries. Rainfall distribution was not good.<br />

The site received a mere 250 mm (normal 330 -488 mm) of rainfall between February (151.3mm) and<br />

March (98.8mm) and poorly distributed. This site had the heaviest infestation of Alectra and is good for<br />

13<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

screening. High insect population was observed at this site as compared to the other sites. Vuli-2<br />

looked good but not outstanding. Entry IT 573-1 doing well and B301 though small seeded. <strong>Crop</strong><br />

management was very good despite drought. IT99K 573-1 and B301had no Alectra in all four<br />

replicates. Two entries, IT99K-7-21-2-2-1 and TZA 263 had only one Alectra plant in one replication.<br />

The third on-station trial was at Hombolo. The entries were same as those planted at Ismani and<br />

Bihawana. The planting date was 16 th January, 2009 and germination occurred on 4 th February, 2009<br />

because of lack of rain after planting. However the Alectra pressure was not high. Field management<br />

was good and the entries performed well.<br />

Seed production: A major objective of this activity was to teach farmers on how to produce quality<br />

seed. The first seed multiplication activity visited in Iringa was at a community plot known as. Three<br />

varieties were planted (Fahari, Vuli-2, and local cultivar). The varieties were planted on 25 th February,<br />

2009 and entries were just starting to flower. The isolation distance was 2.5 meters and crop looked<br />

very clean. Dimethoate was used to control aphids. The soil was quite sandy. Farmers claim late<br />

planting was due to late land preparation because other farmers contributed late for hiring oxen used for<br />

land preparation. There was poor effort in labelling entries.<br />

The second visit was at Mpunguzi, Dodoma region. Two farmers planted one variety (Fahari) on 2 nd<br />

February, 2009. One farmer had a poor field but the other one managed the crop well (one acre) and will<br />

harvest a good yield despite the drought.<br />

Cowpea crossing programme at ARI Ilonga<br />

The visit to see crossing programme was only to Ilonga research institute where the crossing for cowpea<br />

varieties to improve resistance to Alectra is being conducted. This station is in Kilosa district. The<br />

research mandate includes other crops such as maize, sorghum, soybean, sunflower, cotton, pearl millet<br />

besides cowpea. The crossings were done in a screen house and involved commercially released<br />

varieties as recurrent parents in a backcrossing program such as Tumani, Fahari, ,Vuli-1 and Vuli-2.<br />

Aphid infestation is a major problem in the screen house. Backcross 3 seed had been harvested ready for<br />

planting to make backcross 4.<br />

WAY FORWARD/RECOMMENDATIONS:<br />

Drought (rainfall, poor distribution and low amounts) has been an overriding problem this season. We<br />

commend the collaboration the project is undertaking along with the tropical legumes II project of IITA.<br />

The project should therefore incorporate both traits (Alectra resistance and Drought tolerance). An<br />

analysis of medium term climatic data (20-25 years) would be important to determine the frequency of<br />

rainfall events (e.g. start dates, dry spells and rainfall amounts). This can help to determine the<br />

important drought traits in plant (e.g. earliness or resilience). The good production practices should be<br />

incorporated in the screening of the varieties against Alectra. Hence, there is need to expose the groups<br />

involved in the project to all these production practices. There is a need to evaluate the materials<br />

according to maturity group early, medium and late because farmers’ selections are dictated and<br />

influenced by time to maturity. The experiences observed from this project and TLII project indicate<br />

that the overriding preference trait is seed size; large seeded types are more liked than small seeded<br />

types. Thus this should be taken care in our project. The backcrossing programme at Ilonga will<br />

continue to backcross 6 by October 2010, while at Bunda will be at back cross 4. Request for extension<br />

Objective 2: Promotion of high yielding A. vogelii resistant cowpea cultivars<br />

Output 2.1 Farmer and extension awareness of Alectra vogelii and use of resistant cultivars<br />

Activity 2.1.1: Develop leaflets on crop management and ultilization of cowpea, pre-test and print<br />

A draft leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These<br />

were produced in Kiswahili for better understanding by farmers. It emphasized on the different ways of<br />

cowpea utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be<br />

converted into other value-added products such as dhal, stew and snack products like “bagia”. This<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

leaflet has been printed out for further dissemination of the technologies. The leaflet on crop<br />

management is to be produced next cropping season<br />

Activity 2.1.2a: Farmer exchange visits and field days across project sites<br />

Farmer exchange visit involved twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu<br />

(Fahari Group) farmer <strong>Research</strong> Group and also at Ismani On-station trials in Iringa. These were also<br />

accompanied by their extension officers from the respective villages and from the district. The objective<br />

of the farmer exchange visit was farmers from Mangalali to get experiences from other farmers on<br />

principals of cowpea production, processing and utilization as well as how to manage farmer group and<br />

then compare with the way they do in their own group. Also jointly went to research station to learn new<br />

materials and management at the research station.<br />

15<br />

2009<br />

Fig.3. Farmers discuss with their host<br />

about seed production at research plot<br />

of Mangalali <strong>Research</strong> Group, 2009<br />

At Ismani research station, farmers from both Mkungugu and Mangalali participated at evaluation of the<br />

14 cowpea screening materials planted. The outcome from their selection of the lines according to their<br />

criterion is shown in the Table-15Below.<br />

Table 15: Ranking of the cowpea lines planted at Ismani <strong>Research</strong> station 2009<br />

No Cowpea line Number of Number of Ranking of Comment<br />

participants farmers selected the cowpea<br />

line<br />

1 TZA-263 26 24 1 Each farmer was<br />

2 IT99K-7-21-2-<br />

20 2<br />

given a chance<br />

2-1<br />

of selecting only<br />

3 B 301 9 3<br />

best three<br />

4 ITOOK-1207 8 4<br />

cowpea lines<br />

5 IT96D-733 8 4<br />

6 IT89KD-288 6 5<br />

7 IT99K-573-1 3 6<br />

Table 16: Ranking of cowpea by Ari Mpya Farmer <strong>Research</strong> Group Mkungugu 2009<br />

No Cowpea line Number of Number of Ranking of Comment<br />

participants farmers who the cowpea<br />

selected line<br />

1 TZA-263 23 21 1 Each farmer was<br />

2 IT99K-7-21-2-<br />

17 2 given a chance<br />

2-1<br />

of selecting only<br />

3 IT89KD-288 17 2 best three<br />

4 B 301 9 3 cowpea lines<br />

5 IT99K-573-1 5 4<br />

At both sites farmers chose two best cowpea lines, which are TZA 263 for large grain size and IT99K-7-<br />

21-2-2-1 for its earliness.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Activity 2.1.2b Farmers participate at National Agricultural Show in Dodoma 2009:<br />

Two farmers from each of the farmer groups Msungua (Mshikamano FRG) and Ikhanoda (Mfwenji<br />

FRG) organized by the District Council represented their farmer groups at National Agricultural show,<br />

which took place in Dodoma from 1-8 August 2009. The farmers displayed their cowpea varieties and<br />

products processed from cowpea such as bans. This was a good motivation for the farmers.<br />

Output 2.2: Nutrition status of farmers improved<br />

Activity 2.2.1 Inventory of recipes of cowpea products<br />

This activity was undertaken in 2007/08 and completed and made a basis for the following activities<br />

Activity 2.2.2a: Develop/modify / demonstrate cowpea products:<br />

Introduction: Cowpea (Vigina unguiculata) is an important food legume and an integral part of<br />

traditional cropping systems in the semi arid regions of the tropics (Ryan et al, 1984). Although<br />

indigenous to Southern Africa, cowpeas has spread world wide and is extensively cultivated in semiarid<br />

and consumed in many regions of Africa including Tanzania.<br />

Farmers in the project sites produce many varieties of legumes particularly cowpeas, which are<br />

important for food and cash income for household. Thus make leguminous crops rank second after<br />

cereals such as maize and sorghum. Cowpea is a preferred staple food and its desirability reflects the<br />

fact that the leaves, immature pods, fresh seeds, green pods and dry grains can be eaten or marketed.<br />

The dry grains are commonly eaten whole or can be milled and consumed in numerous traditional main<br />

snack foods (E. Sarakikya, 1996). Cowpea grains as well as the vegetable parts, make major nutritional<br />

contributions to diets. Nutritionally, cowpea plays a critical subsistence role in the diets of many<br />

households by providing nutrients that are deficient in cereals.<br />

In order to add value, cowpea grains need to be processed. The processing of cowpea grains improves<br />

palatability, digestability, acceptability, reduced cooking time and increases market value of products<br />

made from dhal. This section therefore, sought to improve cowpeas utilization through nutrients<br />

addition to different products.<br />

The main objective of this study was to promote the utilization of cowpea in Tanzania.<br />

• Train farmers on the processing of cowpea grains into dhal<br />

• Preparation of cowpea dishes (snack bites from cowpea flour “bagia’)<br />

• Perform sensory evaluation tests.<br />

Expected outputs<br />

• Smallholder farmers’ capacity and capability on ways of using processed cowpea products built.<br />

• Smallholder farmers, skills and knowledge in value addition on cowpea products enhanced and<br />

strengthened<br />

• Cowpea products utilization promoted<br />

• Nutrition status for the families in project sites improved<br />

Methedology<br />

Study area<br />

The training was conducted in Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />

Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The sites were<br />

purposively selected as a result of their involvement in a project on “Improvement of cowpea<br />

productivity on Alectra Vogelii infested land in Tanzania.<br />

Materials used<br />

Materials used to perform and conduct this study include, flip charts, board, cowpea grains, cowpea<br />

flour, cowpea dhal, ingredients such as onions, garlic, eggs, chilies, cooking oil and salt.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Approach<br />

A participatory research approach of working with organized village farmer groups was adopted. These<br />

included participatory research models such as demonstrations, displays and training through hands-on<br />

experience. Appreciative problem solving methods were used to encourage farmers learn, explore and<br />

practice new skills and knowledge.<br />

Sample size<br />

The number of farmers in each group varied between 15-35 members. Level of women representation<br />

was 58 percent of the total number. The study participants were mainly smallholder farmers drawn from<br />

eight villages in three regions. These farmers provided the information on producers and consumers.<br />

These farmers mostly grew cowpeas for subsistence and they were the one who tackled the issue of<br />

consumer preference. Other participants included government officials, district and village extension<br />

officers.<br />

Training of farmers and local processors<br />

A one day workshop was conducted in each project area and farmers were trained on improved cowpea<br />

technologies. The trainings were based on demonstrations, hands-on trials and printed materials<br />

(bronchures for cowpea recipes). Farmers participated in the validation of five recipes for cowpea value<br />

addition. Sensory tests were carried out, where by farmers were required to rank and find out the most<br />

preferred type of”bagia”. The five types of bagia were made using different recipes (see Table 1). The<br />

recipes were translated into national language for better understanding.<br />

Table 18: Cowpea snack product recipes<br />

Bagia type Materials/ingredients<br />

1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />

2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />

3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and<br />

water<br />

4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and<br />

water<br />

5. bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs,<br />

yeast and water<br />

Common method used<br />

• Mix all the ingredients together<br />

• Add water and mix well<br />

• Make the dough by needing (allow the dough to rise by leaving it for 10-15 minutes)<br />

• Heat cooking oil until really hot.<br />

• Use spoon or hand to make the size of bagia you need and put the dough into the oil<br />

• Stir and cook until brown<br />

• Remove from oil and drain<br />

• Cool and pack for marketing or save for consumption.<br />

Farmers were equipped with skills and knowledge on quality control with emphasis on the storage<br />

techniques. Also they were informed about product grading/sorting, market requirements of quality,<br />

quantity and sustainability. The training also emphasized on the methods of de-hulling whole cowpea<br />

grain for dhal production that can be converted into other value added and snack products, such as<br />

“bagia”. The value added products are considered nutritious and sources of income generation. It was<br />

however, observed that the prevailing low production levels, lack of adequate and appropriate storage<br />

facilities constrained the sustainability of the niche markets.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Dissemination of information<br />

A Leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These were<br />

produced in Swahili for better understanding of farmers. It emphasized on the different ways of cowpea<br />

utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be converted<br />

into other value-added products such as dhal, stew and snack products like “bagia”. Value-added<br />

products are considered nutritious and sources of income generation. It was also observed that storage of<br />

cowpea grain was a big problem but dehulling the grains could improve storage up to many years. Also<br />

this reduces the labour involved in preparation by women, wherever they come to prepare Bagia.<br />

It is envisaged the disseminated materials can always be referred to by farmers/ processors in case of<br />

problem shooting long after the project expires.<br />

Results and Discussions<br />

Cowpea preference<br />

The study found that, the current type of cowpea grown in the project sites take more than two hours to<br />

cook. The smallholder farmers/consumers prefer cowpeas that cook in less than two hours. Hence<br />

training of cowpea grain processing into dhal as on alternative of reducing cooking time was important.<br />

Sensory evaluation<br />

“Bagia” samples were prepared using the above recipes. Each bagia type reflected to the type of recipe.<br />

At the time of testing the samples were coded 1,2,3,4, and 5 for bagia types made from recipes 1 to 5<br />

respectively. A panel of respective farmers, extension officers and village government leaders<br />

participated in the trial. They were given the five samples of bagia not simultaneously, one at a time and<br />

they were asked to assign preference score on products attributes including taste, texture and<br />

palatability. A pair-wise ranking method (Table19) was used to find out the most preferred bagia type.<br />

Panelists were asked to taste and rank the overall acceptability of the product in relation to that of the<br />

other four. Every sample was compared to each other by allowing every panelist to give opinion or rise<br />

up hands for most preferred sample. The scores of the quality attributes were done using the following<br />

rating scale;<br />

1 = excellent<br />

2 = very good<br />

3 = good<br />

4 = average<br />

5 = poor<br />

The overall acceptability was ranked from 1-5 (Table 19)<br />

Table 19; Ranked cowpea “bagia”<br />

Bagia type Rank<br />

1 5<br />

2 3<br />

3 4<br />

4 2<br />

5 1<br />

Table 2 shows that, bagia type 5 was the most preferred by farmers, while bagia type 1 was the least.<br />

This indicates that, bagia enriched with nutrients (type 5) came out as the most preferred as compared to<br />

“bagia asilia”(type 1), which was not enriched with nutrients (See Table 19). It is evident that,<br />

palatability and taste to a large extent is associated with nutrients enrichment. According to the panelist<br />

views, it is believed that if a product is palatable and nutritionally enriched, it is most likely to fetch<br />

more market than the reverse. Therefore value added products, apart from being nutritious they are<br />

considered palatable and sources of income generation.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 20: Summary village score and rank data<br />

Bagia type Village scores and rank<br />

Singida region Dodoma region Iringa region<br />

Msungua Ikhanoda Nduu Kikombo Mpunguzi Mkungugu Ilambilole Mangalali<br />

S R S R S R S R S R S R S R S R S R<br />

1.Bagia asilia 0 4 0 5 0 5 4 1 0 3 0 5 0 5 0 5 0.5 3.5<br />

2. Bagi +<br />

viungo<br />

3.Bagia +<br />

viungo+hamira<br />

4. Bagia +<br />

viungo+<br />

mayaia<br />

19<br />

2009<br />

Aver<br />

age<br />

Av.<br />

rank<br />

2 2 2 3 4 1 3 2 3 1 2 3 2 3 4 2 2.8 2.1<br />

1 3 1 4 1 4 0 5 1 2 1 4 1 4 1 4 0.9 3.8<br />

4 1 4 1 2 3 1 4 3 1 3 2 3 2 2 3 2.8 2.1<br />

5.Bagia +<br />

viungo+ mayai<br />

+ hamira<br />

4 1 3 2 3 2 2 3 3 1 4 1 5 1 3 1 3.4 1.5<br />

NB: S= Score, R= Rank<br />

Storage and storage insect pests:<br />

Cowpea grain is very susceptible to storage pests especially to bruchids hence store poorly. This has<br />

been one of the constraints that farmers do not keep much of their cowpea grains more than 6 months.<br />

On the other hand dehuled cowpeas are not infested by storage pests and can bee stored for a long time<br />

so long they are kept in a dry place.<br />

CONCLUSIONS AND RECOMMENDATIONS<br />

Initially, cowpea production has mainly been for household use and limited sales to local/village<br />

markets. Thus by providing farmers with the technologies for use in the improvement of the processing<br />

of cowpea into dhal, this study has opened the community to new avenues for storing the cowpea<br />

products longer and obtaining income from the crop.<br />

By achieving the diversification of small-scale rural farmers’ incomes through adoption of appropriate<br />

post-harvest technologies and providing information on the value addition for cowpeas, the project has<br />

equipped farmers with means of enhancing their household incomes. This will be enhanced through<br />

better quality products which will ensure small-holder farmers’ ability to have market access for them.<br />

Additionally, through equipping farmers with knowledge and skills of improved handling, processing<br />

and utilization of cowpea products, the capacity of the rural communities in which the project has<br />

operated, to earn an income from similar processed products through value addition has been enhanced.<br />

Also the project has built the capacity of the rural small-scale cowpea farmers in working in groups and<br />

organizing themselves to produce necessary volumes and quality requirements cowpea grain which can<br />

meet market requirements. The project further enabled the farmers to change their perception of cowpea<br />

as a crop for household consumption and small scale production, to perceiving it as a possible income<br />

earner with potential to increase household incomes.<br />

However, this study highlighted the fact that, what is needed at times is change in attitude of farmers<br />

towards a crop in order to enable them increase its production and make an income from marketing its<br />

products.<br />

It is recommended that, farmer groups need to be facilitated and equipped with means to obtain storage<br />

facilities for large volumes of cowpea grains, especially since large quantities of grains are expected to<br />

be produced.<br />

Finally, it is recommended that, this study is replicated in other areas of the country and other crops<br />

characterized by low utilization base.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Way forward<br />

There a need to have a follow-up study to evaluate post-harvest technologies taught/taken to smallholder<br />

farmers in project sites. This will be a kind of monitoring and evaluation study, it may be done routinely<br />

to evaluate the rate of adoption of post-harvest technologies taught/taken to farmers.<br />

The study will include the following activities;<br />

• Post-harvest technologies taught<br />

• Cowpea handling, processing, storage utilization and marketing<br />

Indicators<br />

- Level and constraints limiting the use of technologies on cowpea handling, processing, storage,<br />

utilization and marketing<br />

- Processing methods<br />

- Storage techniques<br />

- Forms and rate of cowpea consumption at household and community levels<br />

- Cowpea preferences on for example product taste, texture, grain size, skin colour, type of<br />

products, etc<br />

- Cowpea marketing value chains<br />

- Types of value added cowpea products used and sold at the markets, at what type and level of<br />

markets, Access and market linkages established/existing<br />

- The general level, use and application of improved technologies.<br />

References<br />

Eva Pendeli Sarakikya, 1996, Tanzania Cook Book, Tanzania Publishing Limited,<br />

Dar es Salaam.<br />

J.K. Ryan, P.D. Bidinger, N. Prahlad Rao, p.Pushpamma, 1884, The Determinants of<br />

Indivicual Diets and Nutritional Status in six villages of South India, ICRISAT, India<br />

Activity2.2.2b: Analysis of nutrient content in cowpea grain and products<br />

Studies on micronutrients: Minerals of nutritional importance that were analyzed included iron, zinc<br />

and calcium; results indicate that, there was twice much calcium in leaves than in cowpea grains and<br />

almost 40 times as much iron. Since iron and zinc levels in cowpea leaves have shown to be<br />

significantly higher than the levels in cowpea grains, creating more awareness and encouraging the<br />

utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies from cheap sources<br />

especially from resource-poor families. The variety that was best with regards to all minerals was<br />

IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and fats content<br />

were IT97K499‐8 and IT99K‐7‐21‐2‐2‐1.<br />

Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />

seed identified:<br />

Activity 2.3.1: Identification of seed multplication mechanism<br />

- Available sources of seed or classes of seed are<br />

- Breeder seed<br />

- <strong>Foundation</strong> seed<br />

- Certified seed<br />

- Quality Declared Seed (QDS)<br />

From these classes farmer groups or communities can produce QDS from foundation seed or certified<br />

seed. The produced QDS can only be sold within the district. However, only trained Farmer<br />

Groups/Farmers can be contracted by Agricultural Seed Agency (ASA) or any other seed company to<br />

produce certified seed that can be sold beyond the production area.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Sub-Activity2.3.1.1: Train farmers to produce seed (QDS) for commercial use:<br />

Training of farmers on Seed Production (QDS) of Cowpea is very important because the crop saves<br />

many lives of people in drought prone areas of the semiarid areas where the crop is grown. The seed<br />

availability is a big issue because commercial companies are not interested in producing seed of low<br />

volume crops like sorghum, pearl millet bambaranuts and cowpea. For this reason it was embedded in<br />

the project to train farmers to produce their own seed which they can use themselves and sell to the<br />

neighbours and neighbouring villages. It is intended also that once these farmers have known the<br />

techniques of producing high quality seeds, they will later be contracted by the Agricultural Seed<br />

Agency (ASA) to produce certied seed to reach many farmers, and not within their district, but to more<br />

districts that grow the crop.<br />

Farmers were given training on the following main seed production areas:<br />

1. Organization of seed production in Tanzania (L 1): This lecture was given in order to expose farmers<br />

on the various institutions/stakeholders involved in the seed industry, where they are and what they are<br />

doing. It also intended to make them understand the seed classes produced in the country.<br />

Lecture components included the following: The roles of Agriculture <strong>Research</strong> Institutions (ARI’s),<br />

Agricultural Seed Agency (ASA), Seed companies, Farmer groups, Seed Certification Institute and<br />

Agriculture Extension Services Department, Seed production flow from ARI’s to Seed companies and<br />

to farmer groups and Seed classes recognized in Tanzania seed industry<br />

2. Attributes of seed quality (L 2): To make farmers understand the concept of seed quality and its<br />

implications in seed production, processing, storage and distribution. The lecture components included:<br />

Importance of seed as a basic input in agriculture production, Merits of improved varieties as a basic<br />

requirement for any variety before releasing to farmers and Seed quality aspects which are verifiable by<br />

already set standards by Seed Certification Institute such as genetic and physical purity, germination and<br />

seed health.<br />

3. Cowpea Seed Production (L 3): Being the core subject, farmers were trained on the techniques used<br />

in the production of cowpea seeds. More emphasis was put in the husbandry practices, which will<br />

enable them harvest high quality seed.<br />

Training components included: Land selection for cowpea seed production, Land preparation, Choice of<br />

varieties to be planted according to days to maturity, Plant spacing depending on whether a variety is<br />

determinate or indeterminate, <strong>Crop</strong> husbandry practices in general such as pest management, rouging of<br />

off types in seed production, timely harvesting, seed conditioning, safe seed storage and seed sampling<br />

and testing for quality attributes of the project should be initiated by 2010.<br />

Table 17: Summary topics covered by each Farmer <strong>Research</strong> Groups, 2009<br />

Region District Village F R G NO. of<br />

participants<br />

SINGIDA Singida<br />

district<br />

DODOMA Dodoma<br />

urban<br />

IRINGA Iringa<br />

district<br />

Topics Covered<br />

Topic Topic Topic<br />

1 2 3<br />

Msungua Mshikamano 10 X X X<br />

Ikhanoda Mfwenji 20 X X X<br />

Nduu Matumaini 21 X X X<br />

Kikombo Ushirika 11 X X<br />

Mpunguzi Twende na<br />

wakati<br />

10 X X<br />

Mkungugu Ari mpya 10 X X<br />

Mangalali Fahari 14 X X<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 2.4: Cowpea market opportunities identified<br />

Activity 2.4.1: Market survey<br />

The survey was completed 2008 and out of it a paper was presented at the 8 th Scientific Conference of<br />

the Agricultural Economics Society of Tanzania (AGREST) held at Visitors Inn Hotel, Zanzibar, 6 th -8 th<br />

October 2008’<br />

Activity 2.4.2: Marketing chain stakeholders meeting<br />

The meeting was supposed to take place this year but due to poor cowpea harvest, farmers had nothing<br />

to offer to traders. This now has been rescheduled for next season hopping there will be good harvest for<br />

the crop as farmers have enough seed to plant during the season.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 2: Cowpea Utilization in Iringa and Dodoma Regions<br />

Dr. Peter S. Mamiro; Sokoine University of Agriculture, Morogoro<br />

Dr Ambonesigwe. M. Mbwaga, ARI Uyole Mbeya<br />

Introduction<br />

Mature dry cowpeas are important in the diets of many population groups around the world. Africa<br />

alone accounts for 7.5 million hectares of the estimated world total area, of about 10 million hectares.<br />

Of the 7.5 million hectares, about 70% lies in West and Central Africa (Singh et al., 1996). This food<br />

legume is readily available, inexpensive and popular part of the traditional food system. Cowpeas<br />

(Vigna unguiculata L.Walp) along with other legumes are recognized as an important source of protein<br />

(Agazounon et al, 2004). However, their contribution to the overall diet and dietary mineral needs is less<br />

well known. For instance nutritional deficiencies of iron and zinc are often widespread in developing<br />

countries, where staple diets are frequently plant-based and consumption of expensive meat products is<br />

low. Cowpea is an annual legume, cultivated dried fresh grain and long stored dried grain being cooked<br />

while others are dehulled to remove the seed testa, then ground to obtain flour. The flour is used to<br />

make various dishes or recipes. The young and tender cowpea leaves are picked and eaten as relish<br />

along with the main staples. The grains and leaves are the source of carbohydrates, proteins, fats,<br />

vitamin A (ß-carotene), B and C, which are necessary for maintaining good health and fighting disease.<br />

Young cowpea leaves are consumed in at least 18 countries in Africa, and seven countries in Asia and<br />

the Pacific (Ahenkora et al., 1998). Cowpeas are among the top three or four leaf vegetables marketed<br />

and consumed (Barret, 1990). Cowpea also contains iron, calcium, phosphorus, magnesium, copper,<br />

zinc and is high in dietary fibre (Sinha and Kawatra, 2005). The other vegetative parts of the cowpea<br />

plant after removal of the grain, are used as feed, forage, hay and silage for livestock. This paper<br />

reports on the extent of utilization of cowpeas and its contribution to the macro and micro nutrients to<br />

communities that cultivate and consume the legume.<br />

Methodology<br />

Survey<br />

A survey to determine the pattern of utilization of cowpea was conducted in Iringa and Dodoma region<br />

in Kalenga and Msinga villages respectively. The choice of these villages was undertaken in<br />

collaboration with government extension authorities from the two regions. The communities are well<br />

known for the production, selling and consumption of cowpeas. The two regions fall within two<br />

different agro ecological zones,. Iringa being a wet region located in the southern highlands zone and<br />

Dodoma being a semi arid region in the central zone. A simple structured questionnaire seeking<br />

information on the quantity and frequency of consumption of cowpeas and other foods at the homestead<br />

was administered to 511 randomly selected farmers. Samples of local cowpea varieties and products<br />

prepared from cowpeas were collected for analysis in a laboratory at Sokoine University of Agriculture.<br />

The processing and preparation steps of cowpea recipes and products were documented.<br />

Proximate analysis of the samples<br />

The food samples for chemical analysis were grounded to pass through a one millimeter screen in a<br />

Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (% DM) was determined by<br />

drying the sample in an oven at 103 ºC – 105 ºC for 24 hours. Crude protein percentage (% CP) was<br />

determined by Kjeldahl method AOAC method No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030<br />

analyzer, whereby percentage Nitrogen obtained was used to calculate the % CP using the relationship:<br />

% CP = % N x 6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />

System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and percentage ash (%<br />

minerals) was determined immediately after the dry matter determination by incinerating the samples in<br />

a muffle furnace at 550 ºC for four (4) hours. The ash was cooled in a desiccators and then weighed.<br />

Crude fiber percentage (% CF) was determined by using the fiber system and Weende method (AOAC<br />

23<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

1995). Nitrogen free extract percentage (% NFE) was calculated by differences as shown: % NFE = 100<br />

- (% moisture + % CP + % EE + % CF + % Ash).<br />

Total minerals<br />

Total mineral content of the samples was carried out after dry ash. The ash was dissolved in a solution<br />

of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture was 6NHCL. Total iron, zinc<br />

and calcium were determined by atomic absorption spectrophotometry by AOAC method 970.12 No.<br />

(AOAC, 1995).<br />

Data analysis<br />

Data for minerals and proximate analysis were entered in SPSS 12.0 for windows computer software.<br />

Descriptive statistics was used to determine the measures of central tendency for each analyzed cowpea<br />

variety. An analysis of variance of the results was done at 95% confidence interval (P≤0.05) using<br />

Tukeys Honestly Significant Difference. Homogeneity test was performed to determine homogenous<br />

sets.<br />

Results<br />

Demographic characteristics of farmers<br />

There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116 (48%) were<br />

females. In Dodoma 277 farmers were interviewed, 135 (49%) were males and 142 (51%) were females<br />

(Table 1). Farmer’s age ranged between 18 to 83 years with a mean of 45±15 years while in Dodoma<br />

the range was between 20 and 59 years with a mean of 35±7 years. In the two regions, the majority of<br />

the farmers had only studied up to class 7 (57% in Iringa and 88% in Dodoma) which is primary school<br />

level. However, 5% and 1% of interviewed farmers had no formal education in Iringa and<br />

Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up to six family<br />

members.<br />

Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />

Parameter Category Iringa Dodoma<br />

n % N %<br />

Gender Male 124 51.7 135 48.7<br />

Female 116 48.3 142 51.3<br />

Education Class: 3-7 164 70.4 257 92.8<br />

Class 8-14 58 24.2 16 5.9<br />

No education 13 5.4 4 1.4<br />

Household size 1-6 members 192 80.8 183 66.8<br />

7-14 members 48 19.2 94 33.2<br />

Cowpea harvested, procured and utilization<br />

Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own cowpea, or obtained<br />

cowpea supplies from friends and relatives. Few of the farmers purchased cowpea for home<br />

consumption (Table 2). Farmers harvested an average of 75 kg of cowpeas per household with a range<br />

from 53 to 118 kg and the amount sold per household ranged from 4 to 73kg with an average of 25kg<br />

per household. The amount kept at home for consumption ranged from 36 to 97 kg with an average of<br />

52 kg. A substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />

between 100 and 1000 grams of cowpea leaves for household consumption depending on the household<br />

size. Per capital consumption ranged from 40 to 200 gm of cowpea grain for 84% of households in<br />

Iringa and 94% in Dodoma. At the same time per capital consumption for cowpea leaves ranged from<br />

10 to 500 grams by 95% of households in Iringa and 100% in Dodoma, respectively.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

On days when cowpeas are cooked in a household, the same relish will be eaten with either rice or stiff<br />

porridge during lunch, dinner and with breakfast the following morning. More than 50% of households<br />

in Iringa and Dodoma consumed cowpea at least once and up to three times a week (Table 2). Cowpea<br />

leaves were also prepared and eaten on separate days. The leaves are consumed fresh especially during<br />

wet season and in dried form during the lean or dry season. Almost all farmers in the two regions<br />

consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff porridge (ugali), a<br />

mixture of dehulled maize and cowpeas (kande) and most frequently the cowpea buns (bagia).<br />

Table 2: Farmers Responses on Cowpeas Utilization<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Ever eaten cowpea Yes 240 100 277 100<br />

How often eaten per day Twice 92 38.5 156 56.3<br />

Thrice 147 61.5 121 47.7<br />

How often eaten per week Once 77 32.1 28 10.1<br />

Twice 75 31.3 119 43.0<br />

Thrice 65 27.1 82 29.6<br />

Four Times 11 4.6 38 13.7<br />

Five Times 12 5 10 3.6<br />

Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />

Purchase<br />

market<br />

from the 41 17.1 6 2.2<br />

Get from the neighbor 75 31.3 140 50.5<br />

How much cowpea is prepared in one 100-500gms<br />

meal<br />

137 57.1 115 76.9<br />

500-2000gms 103 42.9 64 33.1<br />

How much cowpea leaves are prepared<br />

in one meal<br />

100-1000gms 146 61.9 227 89.7<br />

1000-4000gms 90 39.1 26 11.3<br />

Consumed green cowpea leaves Yes 240 100 277 100<br />

Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />

Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />

20 – 90 minutes 133 65.7 214 79.4<br />

Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />

20 – 90 minutes 31 54.4 72 62.1<br />

How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />

Wash-chop-cook 214 90.7 44 44<br />

Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />

Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />

Cowpea roasted 17 2.1 44 4.8<br />

Roasted milled to flour to prepare<br />

porridge<br />

Cowpea Porridge 28 3.5 66 7.2<br />

Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Parameter Category Iringa Dodoma<br />

Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />

Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />

201-500 39 16.2 19 7.0<br />

Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />

500 - 1400 12 5.1 0 0<br />

Mineral Content<br />

Minerals of nutritional importance that were analyzed included; iron, zinc and calcium. Analysis<br />

showed that all varieties had significantly different (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />

Moisture<br />

(%)<br />

27<br />

Ash<br />

(%)<br />

Ca<br />

(mg/kg)<br />

Zn<br />

(mg/kg)<br />

Fe<br />

(mg/kg)<br />

Local Cowpea grain Dodoma 9.40 3.89 958.14 31.45 28.90<br />

Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />

Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />

Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />

Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />

Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />

Local Cowpea grain Iringa 9.56 3.66 992.43 32.56 27.61<br />

Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />

Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />

Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />

Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />

Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />

Proximate composition<br />

Proximate analysis showed that the cowpea varieties with the highest crude protein content were<br />

IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%) Varieties with lowest iron<br />

included IT00K-1207 (22.01%) and TZA 263 (22.6%). For fats, IT97K499-8 (11.18%), IT99K-7212-2-<br />

1 (10.98%) and Fahari (10.33%) varieties had the highest levels while the lowest included IT97K819-<br />

118 (8.17%) and IT96D-733<br />

(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118 (16.1%). The<br />

lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%) and VULI 2 (13.1%). Among<br />

the best new lines with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1.<br />

Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />

Variety/Food component % DM %CP %CF %EE %NFE<br />

VULI 1 89.25 24.02 13.77 9.12 38.18<br />

IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />

IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />

IT96D-733 88.54 23.44 14.91 8.34 37.68<br />

IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />

IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />

FAHARI 89.29 25.46 13.45 10.33 35.86<br />

TUMAINI 89.13 25.96 13.83 9.89 35.23<br />

IT00K-1207 87.95 22.01 15.66 8.46 37.82<br />

IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />

TZA263 89.21 22.60 17.21 8.86 36.45<br />

VULI 2 89.53 25.64 13.10 10.25 36.53<br />

IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />

B301 90.11 23.75 14.85 8.39 38.85<br />

IT99K-721-2-2-1 89.68 26.00 12.53 10.98 35.89<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />

Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />

Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />

Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />

Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />

Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />

Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />

In comparison with the improved lines and varieties, local cowpeas have comparable levels of dry<br />

matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table 5). However, the levels of<br />

fats are relatively lower (5.4%) in comparison with the improved lines/ varieties ranging from 8% to<br />

11.2%. A similar trend is shown with cowpea leaves. Dehulling the cowpeas reduces crude fibre<br />

significantly with a gradual fall in fats. With regard to the cowpea buns from Iringa and Dodoma<br />

regions there was significant drop in crude protein and crude fibre but a very significant rise in fats<br />

(14.3%).<br />

Discussion<br />

Macro and micro nutrient content<br />

In the two surveyed regions it was revealed that cowpeas are produced by majority of the farmers and<br />

consumed to a relatively large extent by all respondents, which provides an indication of cowpea<br />

importance in the two communities. Similarly, the survey revealed that cowpeas are prepared and<br />

consumed in a number of ways, such as relish for stiff porridge and rice, mixed and boiled with dehulled<br />

or un-dehulled maize and cowpea buns. In both regions, the cowpea buns were important as they were<br />

also a source of income in a number of households. The buns however, appear to have lower iron and<br />

calcium, especially those from Iringa region. The calcium, zinc and iron content of the cowpea buns<br />

were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma region had comparatively<br />

higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and iron (16.3mg/kg). This difference might<br />

emanatre from the method of preparation of cowpeas in Iringa in comparison to Dodoma. In Iringa the<br />

grains are dehulled and soaked for about three hours and then washed in flowing river water for about<br />

an hour. This procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />

these minerals are largely lost during washing.<br />

With regard to the deep frying of the cowpea buns, though some nutrients such as proteins and crude<br />

fibre were significantly reduced (from average of 22% to 19% protein), in the process fats were<br />

significantly increased (from average of 5% to 14%), while nitrogen free extracts and dry matter<br />

remained stable. The oil increase is attributed to the sunflower oil used to deep fry the buns. The farmers<br />

also cultivate sunflower for home use and sale. Greater oil intake is better as daily activities require<br />

adequate energy supply.<br />

Analysis of the developed cowpea varieties showed relatively higher protein levels of 24- 26% and 8%-<br />

11% fat content. Similar results have been observed by Horax et al., (2004) where they found cowpea<br />

seed contained 20-25% protein and was rich in essential and non-essential amino acids. Asante et al.<br />

(2006) were investigating on 32 accessions of cowpea seeds, found protein and tannin contents ranging<br />

from 16.4-27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006) proximate<br />

analysis of various infested cowpea samples indicated increases in protein and crude fiber contents and<br />

decreases in moisture, ash, fats/oil and carbohydrates contents when compared with those of the<br />

uninfected samples. Percentage increases in protein and fiber contents ranged between 9.31-30.56%,<br />

and 1.24-15.11%, respectively while percentage decreases were, moisture (3.97-10.23%); ash (3.75-<br />

12.87%); fats/oil (1.43-10.00%); and carbohydrate (1.88-7.50%).<br />

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Similarly the developed cowpeas were relatively high in calcium, iron, and zinc. However,<br />

considerable mineral variation was found among the analyzed cowpeas varieties. Similar study by<br />

Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea variety with the highest calcium<br />

concentration of 2096.0 g/g and Zn was detected in only few varieties ranging from 1501.0 g/g to<br />

2071.0 g/g. Analysis of variance of the samples showed significant differences (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed varieties,<br />

which are higher yielding and resistant to Alectra, they will probably be accepted by the communities<br />

and the amount per capita of cowpeas consumed should increase provided they continue to cultivate the<br />

same area of land.<br />

Conclusion<br />

Cowpea grain and leaves are widely consumed in the two surveyed areas. The contribution of micro and<br />

macro nutrients is significant for both developed lines and local varieties but with leaves having greater<br />

mineral content than grain. Therefore awareness-raising within communities to consume more cowpea<br />

leaves is required. However the amount produced per household is low compared to requirements.<br />

Consequently cowpea intake per capita is low, and does not meet the RDA. However, since households<br />

in both areas still have ample land they should be encouraged to increase the area planted as well as<br />

planting higher yielding varieties in conjunction with preferred local varieties. This does however<br />

require further market investigation.<br />

Other important nutrients such as vitamins, amino acid profile and levels of ant-nutritional factors<br />

(tannins and phytates) should also be further investigated. The vitamins and amino acids influence in<br />

nutrition while the antinutritional factors influence on the bioavailability of the nutrients in the body.<br />

Acknowlegement<br />

The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong> <strong>Foundation</strong>, who<br />

facilitated successful survey and laboratory analysis of macro and micronutrients.<br />

References<br />

Agazounon, C., Coulibaly, O. and Houndekon, V. (2004) Analysis of of cowpea processing techniques<br />

into ‘atta’ in Benin. Bulletin de la recherché Agronomique du Benin. No. 46, September 2004.<br />

Ahenkora, K. Adu Dapaah, H. K. and Agyeman, A. (1998) Selected nutritional components and sensory<br />

attributes of cowpea (Vigna unguiculata [L.]Walp) leaves Plant Foods for Human Nutrition 52:<br />

221–229.<br />

AOAC (1995), Official methods of analysis. Association of Official Analytical Chemists methods,<br />

AOAC 16 th edition Nos. 963.13, 968.08, 970.12, 915.03, 920.87. Washington, D.C.<br />

Asante, I. K., Adu-Dapaah, H Addison (2006) Seed weight and protein and tannin contents of 32<br />

cowpea accessions in Ghana. Journal of Tropical Science, 44 (2): 77 – 79.<br />

Barret R. P. (1990) Legume species as leafy vegetables. In: Janick J, Simon J. E. (eds), Advances in<br />

new crops. Portland, OR: Timber Press, pp 391–396.<br />

Horax, R., Hettiarachchy, N. S., and Jalaluddin M. (2004).Preparation, characterization and functional<br />

properties of protein isolate from cowpea (Vigna unguiculata L. Walp.) Dept. of Food Science,<br />

Univ. of Arkansas, 2650 N. Young Avenue, Fayetteville, AR AR 72704, (2) Department of<br />

Agriculture, University of Arkansas, Pine Bluff, AR 71611.<br />

http://ift.confex.com/ift/2004/techprogram/paper_22759.htm.Accessed 19.09.2009.<br />

Mgbemena, I. C., Opara, F, N. and Iwuala, M. O. (2006) The effect of insect pest infestation on the<br />

nutritional composition of cowpea and maize. Department of Biotechnology, Federal University<br />

of Technology PMB 1526 Owerri, Nigeria International Journal of Natural and Applied<br />

Sciences 2 (2): 96-100, 2006.<br />

Mune Mune, M. A., Mbome, L. I. and Minka S. R. (2007) Improving the Nutritional Quality of Cowpea<br />

and Bambara Bean Flours for Use in Infant Feeding, Pakistan Journal of Nutrition 6 (6): 660-<br />

664<br />

Olaleke, A. M., Olorunfemi, O. and Akintayo, T. E. (2006) Compositional evaluation of cowpea (Vigna<br />

unguiculata) and scarlet runner bean (Phaseolus coccineus) varieties grown in Nigeria.<br />

International journal of food, agriculture and environment 4 (2): 39‐43.<br />

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Singh B. B., Sharma B. M. and Chambliss O. L. (1996). Recent advances in cowpea breeding. In<br />

Proceedings of the Second World Cowpea <strong>Research</strong> Conference, 5–8 September 1995, Accra,<br />

Ghana. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.<br />

Sinha, R. and Kawatra A. (2005) Effect of Processing on phytic acid and polyphenol contents of<br />

cowpeas (Vigna unguiculata (L) Walp. Plant Foods for human Nutrition, 58 (3): 1‐8.<br />

Wang, N., Hatcher, D.W., Toews, R. and Gawalko, E.J. (2009) Influence of cooking and dehulling on<br />

nutritional composition of several varieties of lentils (Lens culinaris) LWT ‐ Food Science and<br />

Technology, 42, (4): 842‐848.<br />

Weinberger, K., John Msuya, J. and Mamiro, P. (2004) Domestication of selected African indigenous<br />

vegetables in Tanzania – an ex‐ante impact assessment. AVRDC – The World Vegetable Center,<br />

pp22.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 3: Malawi annual report:<br />

Malawi annual report 2008-09 season (third year)<br />

Prepared by V.H. Kabambe, E. Kazila, A. Mangwela, J Bokosi<br />

CC Mtambo, T. Chilongo and E. Mazuma<br />

1. Field evaluation of promising varieties<br />

1.1 Sites and entries.<br />

On farm trials to evaluate performance and adaptation of outstanding lines were conducted in the<br />

2008/09 season. Trial sites were Bunda College in Lilongwe, Mgwangwa EPA in Lilongwe, Mpokwa<br />

EPA in Zomba, Rivirivi EPA in Balaka, Chitedze <strong>Research</strong> Station and Kasungu. Kusungu and Balaka<br />

are new sites which were added in order to consolidate the genotype x environment evaluation, which<br />

would provide strong evidence on adaptation zones for any new entry which we may have to formalize<br />

for release. Balaka is a low altitude site with low and erratic rainfall. Kasungu is a mid-altitude site<br />

with sandy soils. The rainfall was normal and fairly distributed for all sites (Table 1).<br />

Table 1. Mean monthly rainfall (mm) for the trial sites in 2008/09 season.<br />

Month Site<br />

Bunda Ngwangwa Rivirivi Chitedze<br />

October ‘08 18.5 0 3.0 45<br />

November ‘08 119.2 51 113.4 112<br />

December ‘08 177.1 147 242.1 116.4<br />

January ‘09 291.9 198 300.5 227.8<br />

February ‘09 174.0 152 145.1 121<br />

March ‘09 218.3 134 125.1 223.7<br />

April /09 28.0 71 6.3 18.1<br />

May ‘09 0 0 0 0<br />

Monthly total 1026.7 753 932.5 864<br />

Seven of entries selected for evaluation based on Alectra resistance, farmer’s preferences on grain<br />

characteristics and according to evaluations conducted in the last two years. Table 2 gives brief<br />

highlights about the entries based on preliminary yield evaluations. There were about 25 farmers at 4<br />

on-farm sites conducting the trial, each farmer acted as a replicate. At Chitedze and Bunda College the<br />

trials were complete block design, each with 4 replicates. The trial plots had 5 row plots, 4 m long and<br />

0.75 m apart.<br />

Table 2: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />

Code Name Description<br />

5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra<br />

vogelii resistance<br />

37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />

preference<br />

9 IT99K-7-21-2- Best Alectra resistance in ’07, ‘07/08, preferableseed size, , good<br />

2-1<br />

yield<br />

21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium<br />

seed size, low farmer preference<br />

53 Sudan -1 Released variety, good resistance to Alectra and good yielder in<br />

‘07/08<br />

52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08.<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Poor resistance to A. vogelii. Well liked by farmers.<br />

54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />

1.2 Screening for Alectra resistance.<br />

The sites at Ngwangwa and Kasungu had high infestation of Alectra, even though yields were good..<br />

However, within the plots, spots that had high numbers of Alectra were causing stunted growth and<br />

wilting on cowpea plants. Observations on Alectra resistance showed that in sites with high pressure, all<br />

entries succumbed to the parasite, though differentilly. In lightly infested sites, some entries showed<br />

resistance (Table 3). .At Mngwangwa, where pressure was high, the same lines showed fair resistance.<br />

At Lisasadzi, with high Alectra pressure, entries IT97K-825-15 and IT99K-494-6 showed good<br />

resistance. At Bunda College, with high Alectra pressure, the same three lines showed resistance. On the<br />

overall, entry 21 (IT99K-494-6) and entry 9 (IT99K-7-21-2-2-1) were best for resistance with 0.28 and<br />

0.29Alectra plants m -2 respectively, and entry 21 having zero Alectra count at Bunda. Depending on<br />

yield and other results, one of these three could be released. However, all these have longer maturity<br />

periods.<br />

As these observations were done in presence of local extension and farmers, this gave a good<br />

opportunity to orient farmers to the Alectra problem and the objectives of the project. Since the entries<br />

in the trial were combination of early and late maturing types, the general farmers preference at all sites<br />

was for the early types IT82E-16 and Sudan 1. At Kasungu farmers particularly noted that IT97K-825-<br />

15 was undesired due to its late maturity, as it would need more rains. Some of the other reasons for<br />

preferences were grain size (large size preferred).<br />

Table 3 Alectra counts m -2 taken after flowering at all the trial sites<br />

Entrycode<br />

name<br />

& Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />

5=IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />

37=IT97K-825-<br />

15<br />

0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />

9=IT99K-7-21-2-<br />

2-1<br />

0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />

21=IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />

53=Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />

52=IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />

54=Farmers’<br />

local<br />

2.80 1.14 3.29 - 0.03 0.11 1.23<br />

Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

traits in entry 9 and 21 can be reflected in the progenies. The project is so far promoting the released<br />

varieties into its outreach progammes.<br />

Results on number of plants/m 2 are shown in Table 5and they show that there were significant<br />

variations in plant establishment. Looking at yield data, entries 21, 52 and 53 which gave highest yields<br />

also had the best plant establishment.<br />

Table 4:. Grain yield kg/ha of cowpea entries at 6 trial sites in 2008/09.<br />

Entry Site Mean<br />

Lisasadzi Mpokwa Bunda Balaka Mgwangwa Chitedze<br />

5=IT98K-503-1 2034 461 1496 749 856 1667 1210<br />

9=IT97K-7-21-2-<br />

2-1<br />

1749 353 1665 903 543 1222 1073<br />

21=IT99K-494-6 2345 562 1846 1372 1114 972 1369<br />

37=IT99K-825- 1527 447 1276 589 796 1083 953<br />

15<br />

52=IT82E-16 1936 896 1631 2156 903 3194 1786<br />

53=Sudan-1 2328 1402 1728 2158 1070 2278 1827<br />

54=Local - 353 794 964 536 1861 902<br />

Mean 1936 681 1492 1270 831 1754 1327<br />

LSD 523 544 830 878 435 1340<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

9=IT97K-7-21-2-<br />

2-1<br />

10.4 9.0 13.0 10.5 10.0 8.6<br />

21=IT99K-494-6 10.2 11.0 10.8 10.7 11.1 10.8<br />

37=IT99K-825-<br />

15<br />

8.8 10.5 10.7 10.2 10.6 10.2<br />

52=IT82E-16 13.8 13.4 11.5 15.0 14.1 13.5<br />

53=Sudan-1 16.2 17.0 14.8 17.2 16.1 16.3<br />

54=Local - - 16.0 16.0 10.0 14.0<br />

Mean 11.3 11.5 12.3 12.7 12.3 12.0<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

54=Local 3.0 3.5 3.2 4.8 5.0 4.9<br />

Mean 2.5 3.4 3.0 3.7 5.9 4.8<br />

P 0.43 0.60 0.43 0.09<br />

LSD 0.88 1.0 2.40 1.7<br />

CV% 24 21 44 19<br />

Table 9. The effect of genotype on early and late season Cercospora leafspot Incidence (5 plants<br />

affected) and scores (scale 1=clean, 9=severe) of Chitedze <strong>Research</strong> Station.<br />

Entry Cercospora Incidence Cercospora leafspot infection<br />

Early Late Mean Early Late Mean<br />

5=IT98K-503-1 26.6 72.5 49.6 4.2 6.5 5.4<br />

9=IT97K-7-21-2-2-1 21.3 73.8 47.6 5.8 7.3 6.5<br />

21=IT99K-494-6 27.3 68.9 48.1 5.0 7.0 6.0<br />

37=IT99K-825-15 20.8 73.2 47.0 4.8 6.8 5.8<br />

52=IT82E-16 19.2 64.4 41.8 4.8 6.0 5.4<br />

53=Sudan 27.2 75.2 64.4 5.3 7.2 6.3<br />

54=Local 32.7 70.2 75.2 5.8 6.2 6.0<br />

Mean 25.0 71.2 48.1 5.1 6.7 5.9<br />

P 0.38 0.80 0.44 0.14<br />

LSD 13.3 15.2 1.6 1.0<br />

CV% 36 23 22 11<br />

1.5 Days to flowering and maturity<br />

The variations of cowpea entries on days to 50% flower and to maturity are shown in Table 10 and 11.<br />

Table 10 shows that on average plants flowered earliest at Mpokwa (46 days from planting), closely<br />

followed by Mngwangwa (47 days). Mean date to flowering was most delayed at Lisasadzi in Kasungu,<br />

mainly due to the influence of the local variety which took 89 days to flower. In general Sudan-1 was<br />

the earliest to flower across all sites, followed by IT82E-16. The local entry was the last to flower at<br />

Mngwangwa and Lisasadzi only. In general, there were small differences between flowering dates of the<br />

improved varieties (no more than 10 days), but the farmers highlighted that these differences were<br />

significant and important. Regarding days to maturity, the mean maturity period was shortest at<br />

Mpokwa and Balaka, which was expected. These sites are warmer and drier historically. Apart from<br />

the local entry, entries 9 and 37 had the longest maturity periods of about 77 days. This agrees with<br />

observations in the fields and farmers reasons for disliking the entries. Although these entries were late,<br />

they were inferior in yield compared to the early maturing released varieties. This further suggests good<br />

adaptability for the released varieties.<br />

Table 10. Effect of genotype on days to 50% flower at 5 sites, 2008/09<br />

Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />

5=IT98K-503-1 46 52 57 46 48 50<br />

9=IT97K-7-21-2-2-1 48 54 59 53 53 53<br />

21=IT99K-494-6 46 52 57 46 47 50<br />

37=IT99K-825-15 47 56 58 53 52 53<br />

52=IT82E-16 44 48 55 39 46 46<br />

53=Sudan-1 46 47 59 41 51 49<br />

54=Local 50 53 89 - 52 61<br />

Mean 47 52 62 46 50 51<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

CV% 4 4 9 7 26<br />

Table 11 Effect of cowpea line on days to maturity at five sites, 2008/09 season.<br />

Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />

5=IT98K-503-1 79 82 73 64 61 72<br />

9=IT97K-7-21-2-2-1 80 83 76 73 70 76<br />

21=IT99K-494-6 80 82 71 65 60 72<br />

37=IT99K-825-15 83 88 72 74 69 77<br />

52=IT82E-16 74 75 60 59 66 67<br />

53=Sudan-1 74 74 68 60 69 69<br />

54=Local 80 83 107 - 68 84<br />

Mean 78 81 76 66 66 73<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 13. Proportion of farmers preferences of trial lines based on selected criteria<br />

Entry Criteria Site or village<br />

Bala Bunda Mngwa Mpokwa Mpokwa Lisaka<br />

ngwa Khuvinda CChibisa Sadzi<br />

vge Vge<br />

n 24 40 17 40 39 32<br />

5=IT98K-503-1 Seed size 4 100 100 90 10 34<br />

Plant type 0 100 100 5 0 59<br />

Early maturity 0 100 100 0 100 3<br />

Insect resistant 0 100 100 0 0 28<br />

Disease resistant 0 100 100 0 25 38<br />

9=IT97K-7-21-2-2-<br />

1<br />

Seed size 100 100 65 60 100 53<br />

Plant type 100 40 41 35 0 63<br />

Early maturity 0 100 47 0 0 0<br />

Insect resistant 71 100 100 0 0 0<br />

Disease resistant 71 100 100 0 0 0<br />

21=IT99K-493-6 Seed size 100 25 71 55 60 50<br />

Plant type 4 0 88 0 26 81<br />

Early maturity 100 0 41 45 67 100<br />

Insect resistant 0 0 100 0 44 100<br />

Disease resistant 0 0 100 0 21 63<br />

37=IT99K-825-15 Seed size 100 70 0 100 38 0<br />

Plant type 0 100 41 15 0 44<br />

Early maturity 0 83 0 0 0 0<br />

Insect resistant 0 100 100 25 0 0<br />

Disease resistant 0 100 100 15 0 13<br />

52=IT82E-16 Seed size 71 35 91 75 28 59<br />

Plant type 100 40 100 100 49 63<br />

Early maturity 100 55 100 100 62 81<br />

Insect resistant 0 60 100 100 40 57<br />

38<br />

2009<br />

Disease resistant 0 50 100 100 26 100<br />

Seed size 75 83 88 75 21 82<br />

53=Sudan Plant type 100 100 100 100 49 68<br />

Early maturity 100 100 100 100 59 100<br />

Insect resistant 100 100 100 100 59 100<br />

Disease resistant 100 15 100 100 77 59<br />

Farmers local Seed size 100 50 0 65 39 19<br />

Plant type 100 0 0 100 41 19<br />

Early maturity 0 75 0 0 0 0<br />

Insect resistant 0 0 0 100 72 0<br />

Disease resistant 0 0 0 100 62 0<br />

1.7 GENERAL DISCUSSION<br />

The results of the field test have been informative due to close association between yield data, yield<br />

components and agronomic traits. The interesting point is that the earlier maturing released varieties<br />

gave highest yields. In general, longer maturity varieties are expected to give higher yields, the reason<br />

for low yields could be low yield potential, or uncontrolled insect pest attack. The key observations on<br />

the entries are summarized in Table 14 below. All entries were equally prone to pests and diseases. The


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

high yields recorded under famer’s conditions are encouraging for them to grow cowpea for food and<br />

income generation.<br />

Table 14. Key traits and strengths of trial entries<br />

Entry Key traits and strength<br />

5=IT98K-503-1 Low yielder, medium maturity (64 days), disease prone, poor farmer<br />

preference, medium Alectra resistance<br />

9=IT97K-825-15 Large seed size, low yielder, good Alectra resistance, medium farmer<br />

preference, long maturity period (73 days), disease and pest prone<br />

21=IT99K-7-21-2-2- Good seed size, medium maturity (65 days), very good Alectra resistance,<br />

1<br />

prone to pests, earmarked for release.<br />

37=IT99K-494-6 Poor farmer preference, late maturity (74 days), very good Alectra<br />

resistance, poor yielder, prone to disease and insect pests<br />

52=Sudan-1 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />

maturity, released variety<br />

53=IT82E-16 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />

maturity, released variety<br />

54=Local Variable traits depending on source, largely late maturing, pest prone,<br />

medium yields<br />

2. INCORPORATION OF ALECTRA RESISTANCE INTO ADAPTED VARIETIES<br />

This work is in progress. The released lines are being upgraded to incorporate Alectra vogelii resistance.<br />

The donor lines for Sudan-1 are IT97K-820-18, IT99K-1060, IT99K494-6, IT97K-7-21-2-2-1, and<br />

IT93K-452-1. The donor linefor IT82E-16 is 1T81D-994. The work is at backcross 2 stage to be<br />

followed by selfing.<br />

3. COLLABORATIVE STUDENT WORK<br />

3.1 BSc student (Cowpea variety x time of planting x density trial)<br />

During the season an undergraduate student undertook a study on cowpea time of planting x variety x<br />

density trial. The objective was to determine how these factors interact to optimize crop yields with<br />

respect to rainfall and the need to plant late to avoid disease and insect pressure. Preliminary<br />

observations showed that early planting was the best for yield and less disease infection. This was<br />

because there was a dry spell soon after the first planting. This might have led to conditions not<br />

favourable for the build up of the diseases.<br />

3.2: MSC Student (Ms Elida Kazira - Farmer Participatory Selection of Alectra Resistant Cowpea<br />

Varieties in Selected Districts of Malawi).The student has submitted her thesis which is in the hands of<br />

examiner.<br />

4. CAPACITY BUILDING AND SCALING OUT OF IMPROVED COWPEA LINES<br />

4.1 Primary seed support<br />

The project provided small packs of seed of 0.5 kg each to all project participating farmers Mpokwa and<br />

Mngwangwa EPA (about 60 farmers at each EPA). Participating farmers in Kasungu were also given<br />

this support. Many farmers planted this in pure stand while others planted this in intercrop with maize.<br />

The objective of this program was to provide an experience for the farmers to grow cowpea intensively<br />

(as sole crop) and to practice pest management and observe results. Some of the farmers we monitored<br />

managed to get good yields and were motivated to produce cowpeas. We have helped these farmers to<br />

store some of their seeds so that they can plant more cowpeas next season. During field days and visits<br />

farmers were trained on principles of cowpea seed production. Farmers were also encouraged to use<br />

their cowpea to practice the different recipes which they learned from the project.<br />

4.2 Agriculture fair participation and sharing of cowpea recipes<br />

In June 2009 the farmers from Mngwangwa EPA, on their own accord, organized themselves to<br />

participate at a District Agriculture Fair held in the same EPA. The farmers demonstrated a range of<br />

39<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

cowpea products based on the training made by the project and shared this with participants (Figure 1).<br />

In addition, the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science<br />

Department. Again, the focus of the exhibit was of utilization. The exhibits attracted a lot of attention<br />

from participants.<br />

Figure 1. Farmer group displays of cowpea recipes (left) and cross section of participants (right)<br />

at ADD – wide show in Lilongwe.<br />

4.3 Group dynamics training for farmers in Ngwangwa<br />

Group dynamics training was conducted for all the farmers in the two participating villages in<br />

Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />

leadership and operation. This group strengthening included membership and responsibilities,<br />

constitution, networking and fund raising to sustain the groups.<br />

4.4 Workshops on cowpea market value chain<br />

Cowpea market value chain workshops were conducted in Lilongwe and Zomba in August 2009. These<br />

workshops were held following the conclusion of a market survey. As per project document, the,<br />

meetings were held to bring together representatives of cowpea producers, middlemen and traders. The<br />

objective was to link producers better to the market and identifies increased marketing opportunities.<br />

There were lead presentations made by project team theme leaders as follows:- a) a presentation on<br />

cowpea production trends, production requirements, cowpea agronomy, potential yields reasons for low<br />

yields in Malawi and cowpea storage Mr E. Mazuma; b) a presentation on cowpea utilization, including<br />

nutritional value, processing of leaves and grain, utilization as animal feed by Dr Mangwela and c)<br />

presentation on cowpea preferences and marketing by Mr Thabie Chilongo. At both venues there were<br />

representatives of farmers, government extension, NGO’s, agricultural input suppliers, traders and<br />

researchers.<br />

The workshop revealed that there are six big business traders who buy cowpea seeds (ADMARC, Rab<br />

Processors, Mulli Brothers, Agora, Grain Traders Association, and Transglobe). Many of them placed<br />

emphasis on large seeded grain types as preferences. A representative of ADMARC indicated that this<br />

season they have been instructed to buy large seeded cowpea only. The traders acknowledged that they<br />

don’t buy much cowpea, but that the markets were small as well thus can satisfy it. Some cowpea is<br />

sold locally (for cakes and cookie making) while most of it is exported. However, it was noted that most<br />

of the cowpeas are traded in local markets with no clear records on volumes and quality requirements.<br />

At the meetings we displayed a draft version of our booklet on cowpea production and utilization. The<br />

participants demanded speedy release of this book. The nutrition officers collected the draft copies for<br />

immediate use. The workshops also noted that there are many reasons for variety choice amongst<br />

40<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

farmers, such as maturity period, plant stature (whether climbing or creeper), seed size, stay green<br />

characteristics (for prolonged leaf harvesting), seed size and pest resistance. Participants resolved that<br />

some of these preferences were minor and suitable for backyard crop for home consumption. It was thus<br />

resolved that researchers should focus on high yield and pest resistance and commercially desirable<br />

traits such as large seeds. Farmers should be advised to plant small patches of local varieties for<br />

vegetables and green pods.<br />

At the Lilongwe Workshop the research team established links with IITA, which has just won a grant to<br />

work on cowpea. The initial collaboration will cover scaling out issues and market and preferences<br />

surveys. At the Zomba workshop we established a partnership with World Vision Area Development<br />

project officer to facilitate seed supply for groups of farmers for scaling out. They already have<br />

experience on community-based seed production. Similar relationships were established with the<br />

District Agriculture Development Officer for Phalombe.<br />

4.5 Cowpea market survey<br />

This work has been conducted and results are being analyzed, a separate report will be prepared. Very<br />

preliminary results were shared during workshops on cowpea market value chain.<br />

4.6. Policy workshop on seeds<br />

The Malawi country lead participated in a workshop bringing together relevant policy officials to<br />

discuss issues that can facilitate the scaling out of legume seed systems and Integrated Soil Fertility<br />

Management. The workshop was jointly organized by the <strong>McKnight</strong> Legume Best Bets and Seed<br />

Systems projects.<br />

The major policy issue on seed systems, which was a matter of concern, was the seed registration and<br />

certification process. The key concern was t based on the fact that process is too centralized. A seed<br />

grower is required to register at Chitedze <strong>Research</strong> Station, and only the same can certify. The<br />

registration fee is high, particularly for small holder farmers or community group whose cultivated area<br />

is 1-2 ha. The registration fees and inspection costs will erode profits. The argument was that legumes,<br />

self pollinated, have simple requirements for isolation, and rouging for disease and off-types can be<br />

done at District level. Any seed not going through the process cannot be packed and sold on shelves,<br />

then killing any entrepreneurship. The dissemination of legume seeds is directly linked ISFM because<br />

the same do make some N additions to soil. These concerns and other suggestion were raised for<br />

consideration by policy makers.<br />

5: Way forward<br />

Based on the above, the project team would like to make the following recommendations for future<br />

research action.<br />

1. Evaluate the lines again this season and earmark entry 21 for release owing to its good Alectra<br />

tolerance and medium yield<br />

2. Noting the lack of resistance to disease and pests across lines, to intensity the work on crop<br />

protection. This work should include clear definitions of critical minimum sprays required to<br />

keep pesticide costs and labour down. Also to intensity studies on use of botanical pesticides<br />

such as neem, moringa, Tephrosia vogelii and others.<br />

3. To refine the issues of time of planting in line with variety maturity periods in order to avoid<br />

pests and for drought management purposes.<br />

4. To intensify incorporation of resistance into the adapted lines and conduct adaptation trials.<br />

5. To extend primary seed support programme to all testing sites and to willing partners.<br />

6. To request for extension of the project with the objective of completing tasks above. To release<br />

new Alectra resistant at the end of renewal, as well as to establish a seed base to support the<br />

scaling out of the new varieties.<br />

41<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

42<br />

2009<br />

7. Continue with introgression of Alectra resistance in adapted cowpea varieties at Bunda College<br />

if extension of the project has been granted. At the end of the first phase we shall have reached<br />

backcross four.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />

Team Report<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Project leader: Dr AM Mbwaga, ARI Uyole, Box 400 Mbeya, Tanzania. Email: ambwaga@yahoo.co.uk<br />

Team report for 2008 ‐ 2009<br />

Team activities<br />

Team members from Malawi and Tanzania had a number of opportunities to meet during the year.<br />

These included the CoP2 meeting in Maputo, Mozambique in October 2008. This event allowed<br />

representatives from each country to discuss progress and were followed up with a visit by the project<br />

leader Dr Mbwaga accompanied by Dr Mligo, legume breeder, Dr Charlie Riches (CoP- liaison officer) to<br />

Lilongwe in March 2009 and met by the Malawian project team that was composed of Dr Vernon<br />

Kabambe country project leader, Mosses Mamiliro (breeder), and James Bokosi (breeder). The team<br />

visited several project sites, which included Lilongwe district in Ngwangwa Extension Planning Area<br />

(EPA), Kasungu district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi<br />

EPA where farmer groups are undertaking on‐farm trials with the project. The wrap up discussions at<br />

Bunda College, University of Malawi came up with the following observations/recommendations; -<br />

released varieties showed better yields than test lines, early maturing varieties save as source of food in<br />

hunger periods, seed system for cowpea is very poor, more cowpea seed should be sent to Balaka and<br />

Kasungu districts as there was high demand for seed of the improved cowpea varieties and lastly farmers<br />

in Rivirivi were recommended to form farmer research groups for better access by researchers and<br />

extension staff as compared to individual isolated farmers<br />

Representatives of project partner institutions travelled together to assess progress with project<br />

activities in Tanzania starting on 27 th April, 2009 in Iringa and ending on 30th April at Ilonga Agricultural<br />

<strong>Research</strong> Institute, Kilosa in Tanzania. Evaluation team members were: Joseph Mligo (Breeder, ARI<br />

Ilonga Kilosa Tanzania); Vernon Kabambe (co‐ordinator for Malawi, Bunda College Malawi); James<br />

Bokosi (Breeder, Bunda College of Malawi), Boukar Ousmane (cowpea breeder IITA Kano Nigeria) and<br />

Ambonesigwe Mbwaga (Project leader, ARI Uyole Mbeya Tanzania). The team was joined by Extension<br />

staff working with the project and other staff members at various sites in each district of Tanzania. From<br />

the evaluation it was noted that drought (rainfall, poor distribution and low amounts) has been an<br />

overriding problem this season. The team commended the collaboration the project is undertaking along<br />

with the tropical legumes II project of IITA. It was recommended to incorporate both traits (Alectra<br />

resistance and Drought tolerance) in the breeding programme. An analysis of medium term climatic data<br />

(20-25 years) would be important to determine the frequency of rainfall events (e.g. start dates, dry spells<br />

and rainfall amounts). This can help to determine the important drought traits in plants (e.g. earliness or<br />

resilience). The good production practices should be incorporated in the screening of the varieties against<br />

Alectra. Hence, there is a need to expose the farmer groups involved in the project to all these production<br />

practices.<br />

The experiences observed from cowpea/Alectra and TL II projects indicate that the overriding preference<br />

trait by farmers is seed size; large seeded are more liked than small seeded types.<br />

The backcrossing programme at ARI Ilonga will continue to backcross 6 by October 2010, while at<br />

Bunda College will be at back cross 4. There will be an urgent need to request for an extension of the<br />

project as the intended cowpea materials will have not reached the farmers at the end of the funding year<br />

2010.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />

Team Report<br />

Insights and lessons learned<br />

During the monitoring tour the team found that the activities planned for 2009 were underway. This was<br />

continuation of activities started in year 1 and 2, including breeding for Alectra resistance and on-farm<br />

evaluation of promising cowpea lines, market surveys, cowpea processing and utilisation as well as new<br />

work on cowpea macro and micro nutrient analysis in cowpea products. Also introduced to the project<br />

was the training of farmers on seed production i.e. production of Quality Declared Seeds (QDS).<br />

There was an outbreak of cutworms on the demonstrations planted in Singida and this gave a learning<br />

lesson that cowpea seed should be dressed with insecticide to prevent damage by cutworms especially at<br />

two leaf stage.<br />

Variability was observed in the reaction of individual cowpea lines to Alectra across sites. Seed was<br />

therefore provided from a range of locations in both countries for a glasshouse screening trial at Long<br />

Ashton UK. This confirmed variability in the host range of parasite samples from Malawi and Tanzania<br />

At a species level there are 3 strains of Alectra by host range: Alectra from Singida in Tanzania attaches<br />

and emerges on cowpea and groundnut but not on mung or common bean. Alectra from Bihawana and<br />

Ismani in Tanzania attaches and emerges on cowpea, groundnut, and common bean but not on<br />

mung bean. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and<br />

mung bean. Within cowpea, patterns of virulence are evident: Alectra from all sites in both countries<br />

emerges on IT36E-16 and TZA 263 but not on B301. There is need to confirm this by using<br />

biotechnology techniques.<br />

Three selected cowpea lines with traits farmers like, including IT99K-7-21-2-2-1, IT99K-753-1 and TZA<br />

267 are being increased under irrigation at Ilonga <strong>Research</strong> Station to be distributed to many farmers for<br />

wider evaluation in the 2009/2010 season<br />

Due to poor cowpea crop performance during this season, the intended market stakeholder’s meeting<br />

was postponed to next season in Tanzania but it was undertaken in Malawi and a report is being<br />

prepared.<br />

A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />

Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual Agricultural<br />

Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in AGREST conference<br />

series Vol. 8. (in press). The proceedings will be ready within and outside the country. Mr Gabriel<br />

Martin’s got financial support from the project for his MSc research work


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Cowpea Utilization in Iringa and Dodoma Regions<br />

Peter S. Mamiro 1 , A. M. Mbwaga 2<br />

Introduction<br />

Mature dry cowpeas are important in the diets of many population groups around the<br />

world. Africa alone accounts for 7.5 million hectares of the estimated world total area, of<br />

about 10 million hectares. Of the 7.5 million hectares, about 70% lies in West and Central<br />

Africa (Singh et al., 1996). This food legume is readily available, inexpensive and<br />

popular part of the traditional food system. Cowpeas (Vigna unguiculata L.Walp) along<br />

with other legumes are recognized as an important source of protein (Agazounon et al,<br />

2004). However, their contribution to the overall diet and dietary mineral needs is less<br />

well known. For instance nutritional deficiencies of iron and zinc are often widespread in<br />

developing countries, where staple diets are frequently plant-based and consumption of<br />

expensive meat products is low. Cowpea is an annual legume, cultivated dried fresh grain<br />

and long stored dried grain being cooked while others are dehulled to remove the seed<br />

testa, then ground to obtain flour. The flour is used to make various dishes or recipes. The<br />

young and tender cowpea leaves are picked and eaten as relish along with the main<br />

staples. The grains and leaves are the source of carbohydrates, proteins, fats, vitamin A<br />

(ß-carotene), B and C, which are necessary for maintaining good health and fighting<br />

disease. Young cowpea leaves are consumed in at least 18 countries in Africa, and seven<br />

countries in Asia and the Pacific (Ahenkora et al., 1998). Cowpeas are among the top<br />

three or four leaf vegetables marketed and consumed (Barret, 1990). Cowpea also<br />

contains iron, calcium, phosphorus, magnesium, copper, zinc and is high in dietary fibre<br />

(Sinha and Kawatra, 2005). The other vegetative parts of the cowpea plant after removal<br />

of the grain, are used as feed, forage, hay and silage for livestock. This paper reports on<br />

the extent of utilization of cowpeas and its contribution to the macro and micro nutrients<br />

to communities that cultivate and consume the legume.<br />

Methodology<br />

Survey<br />

A survey to determine the pattern of utilization of cowpea was conducted in Iringa and<br />

Dodoma region in Kalenga and Msinga villages respectively. The choice of these villages<br />

was undertaken in collaboration with government extension authorities from the two<br />

regions. The communities are well known for the production, selling and consumption of<br />

cowpeas. The two regions fall within two different agro ecological zones,. Iringa being a<br />

wet region located in the southern highlands zone and Dodoma being a semi arid region<br />

in the central zone. A simple structured questionnaire seeking information on the quantity<br />

and frequency of consumption of cowpeas and other foods at the homestead was<br />

1<br />

1.Department of Food Science and Technology, Sokoine University of Agriculture, P. O. Box 3006,<br />

Morogoro, Tanzania.<br />

2 Uyole Agricultural <strong>Research</strong> Institute, Box 400, Mbeya, Tanzania


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

administered to 511 randomly selected farmers. Samples of local cowpea varieties and<br />

products prepared from cowpeas were collected for analysis in a laboratory at Sokoine<br />

University of Agriculture. The processing and preparation steps of cowpea recipes and<br />

products were documented.<br />

Proximate analysis of the samples<br />

The food samples for chemical analysis were grounded to pass through a one millimeter<br />

screen in a Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (%<br />

DM) was determined by drying the sample in an oven at 103 ºC – 105 ºC for 24 hours.<br />

Crude protein percentage (% CP) was determined by Kjeldahl method AOAC method<br />

No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030 analyzer, whereby percentage<br />

Nitrogen obtained was used to calculate the % CP using the relationship: % CP = % N x<br />

6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />

System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and<br />

percentage ash (% minerals) was determined immediately after the dry matter<br />

determination by incinerating the samples in a muffle furnace at 550 ºC for four (4)<br />

hours. The ash was cooled in a desiccators and then weighed. Crude fiber percentage (%<br />

CF) was determined by using the fiber system and Weende method (AOAC 1995).<br />

Nitrogen free extract percentage (% NFE) was calculated by differences as shown: %<br />

NFE = 100 - (% moisture + % CP + % EE + % CF + % Ash).<br />

Total minerals<br />

Total mineral content of the samples was carried out after dry ash. The ash was dissolved<br />

in a solution of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture<br />

was 6NHCL. Total iron, zinc and calcium were determined by atomic absorption<br />

spectrophotometry by AOAC method 970.12 No. (AOAC, 1995).<br />

Data analysis<br />

Data for minerals and proximate analysis were entered in SPSS 12.0 for windows<br />

computer software. Descriptive statistics was used to determine the measures of central<br />

tendency for each analyzed cowpea variety. An analysis of variance of the results was<br />

done at 95% confidence interval (P≤0.05) using Tukeys Honestly Significant Difference.<br />

Homogeneity test was performed to determine homogenous sets.<br />

Results<br />

Demographic characteristics of farmers<br />

There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116<br />

(48%) were females. In Dodoma 277 farmers were interviewed, 135 (49%) were males<br />

and 142 (51%) were females (Table 1). Farmer’s age ranged between 18 to 83 years with<br />

a mean of 45±15 years while in Dodoma the range was between 20 and 59 years with a<br />

mean of 35±7 years. In the two regions, the majority of the farmers had only studied up<br />

to class 7 (57% in Iringa and 88% in Dodoma) which is primary school level. However,<br />

5% and 1% of interviewed farmers had no formal education in Iringa and


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up<br />

to six family members.<br />

Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Gender Male 124 51.7 135 48.7<br />

Female 116 48.3 142 51.3<br />

Education Class: 3-7 164 70.4 257 92.8<br />

Class 8-14 58 24.2 16 5.9<br />

No education 13 5.4 4 1.4<br />

Household size 1-6 members 192 80.8 183 66.8<br />

7-14 members 48 19.2 94 33.2<br />

Cowpea harvested, procured and utilization<br />

Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own<br />

cowpea, or obtained cowpea supplies from friends and relatives. Few of the farmers<br />

purchased cowpea for home consumption (Table 2). Farmers harvested an average of 75<br />

kg of cowpeas per household with a range from 53 to 118 kg and the amount sold per<br />

household ranged from 4 to 73kg with an average of 25kg per household. The amount<br />

kept at home for consumption ranged from 36 to 97 kg with an average of 52 kg. A<br />

substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />

between 100 and 1000 grams of cowpea leaves for household consumption depending on<br />

the household size. Per capital consumption ranged from 40 to 200 gm of cowpea grain<br />

for 84% of households in Iringa and 94% in Dodoma. At the same time per capital<br />

consumption for cowpea leaves ranged from 10 to 500 grams by 95% of households in<br />

Iringa and 100% in Dodoma, respectively.<br />

On days when cowpeas are cooked in a household, the same relish will be eaten with<br />

either rice or stiff porridge during lunch, dinner and with breakfast the following<br />

morning. More than 50% of households in Iringa and Dodoma consumed cowpea at least<br />

once and up to three times a week (Table 2). Cowpea leaves were also prepared and<br />

eaten on separate days. The leaves are consumed fresh especially during wet season and<br />

in dried form during the lean or dry season. Almost all farmers in the two regions<br />

consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff<br />

porridge (ugali), a mixture of dehulled maize and cowpeas (kande) and most frequently<br />

the cowpea buns (bagia).


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Table 2: Farmers Responses on Cowpeas Utilization<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Ever eaten cowpea Yes 240 100 277 100<br />

How often eaten per day Twice 92 38.5 156 56.3<br />

Thrice 147 61.5 121 47.7<br />

How often eaten per week Once 77 32.1 28 10.1<br />

Twice 75 31.3 119 43.0<br />

Thrice 65 27.1 82 29.6<br />

Four Times 11 4.6 38 13.7<br />

Five Times 12 5 10 3.6<br />

Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />

Purchase from the market 41 17.1 6 2.2<br />

Get from the neighbor 75 31.3 140 50.5<br />

How much cowpea is prepared in one meal 100-500gms 137 57.1 115 76.9<br />

500-2000gms 103 42.9 64 33.1<br />

How much cowpea leaves are prepared in one<br />

meal<br />

100-1000gms 146 61.9 227 89.7<br />

1000-4000gms 90 39.1 26 11.3<br />

Consumed green cowpea leaves Yes 240 100 277 100<br />

Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />

Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />

20 – 90 minutes 133 65.7 214 79.4<br />

Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />

20 – 90 minutes 31 54.4 72 62.1<br />

How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />

Wash-chop-cook 214 90.7 44 44<br />

Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />

Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />

Cowpea roasted 17 2.1 44 4.8<br />

Roasted milled to flour to prepare porridge Cowpea Porridge 28 3.5 66 7.2<br />

Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />

Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />

Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />

201-500 39 16.2 19 7.0<br />

Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />

500 - 1400 12 5.1 0 0


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Content<br />

Minerals of nutritional importance that were analyzed in cowpea products included iron,<br />

zinc and calcium. Analysis showed that all varieties had significantly different (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />

Moisture<br />

(%)<br />

Ash<br />

(%)<br />

Ca<br />

(mg/kg)<br />

Zn<br />

(mg/kg)<br />

Fe<br />

(mg/kg)<br />

Local Cowpea Dodoma 9.40 3.89 958.14 31.45 28.90<br />

Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />

Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />

Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />

Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />

Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />

Local Cowpea Iringa 9.56 3.66 992.43 32.56 27.61<br />

Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />

Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />

Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />

Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />

Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />

Can we explain the difference from the two sites<br />

Proximate composition<br />

Proximate analysis showed that the cowpea varieties with the highest crude protein<br />

content were IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%)<br />

Varieties with lowest iron included IT00K-1207 (22.01%) and TZA 263 (22.6%). For<br />

fats, IT97K499-8 (11.18%), IT99K-7212-2-1 (10.98%) and Fahari (10.33%) varieties had<br />

the highest levels while the lowest included IT97K819-118 (8.17%) and IT96D-733<br />

(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118<br />

(16.1%). The lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%)<br />

and VULI 2 (13.1%). Among the best new lines with regards to crude protein and fats<br />

content were IT97K499-8 and IT99K-7212-2-1.<br />

Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />

Variety/Food component % DM %CP %CF %EE %NFE<br />

VULI 1 89.25 24.02 13.77 9.12 38.18<br />

IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />

IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />

IT96D-733 88.54 23.44 14.91 8.34 37.68<br />

IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />

IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />

FAHARI 89.29 25.46 13.45 10.33 35.86<br />

TUMAINI 89.13 25.96 13.83 9.89 35.23<br />

IT00K-1207 87.95 22.01 15.66 8.46 37.82


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />

TZA263 89.21 22.60 17.21 8.86 36.45<br />

VULI 2 89.53 25.64 13.10 10.25 36.53<br />

IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />

B301 90.11 23.75 14.85 8.39 38.85<br />

IT99K-7212-2-1 89.68 26.00 12.53 10.98 35.89<br />

Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />

Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />

Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />

Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />

Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />

Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />

Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />

In comparison with the improved lines and varieties, local cowpeas have comparable<br />

levels of dry matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table<br />

5). However, the levels of fats are relatively lower (5.4%) in comparison with the<br />

improved lines/ varieties ranging from 8% to 11.2%. A similar trend is shown with<br />

cowpea leaves. Dehulling the cowpeas reduces crude fibre significantly with a gradual<br />

fall in fats. With regard to the cowpea buns from Iringa and Dodoma regions there was<br />

significant drop in crude protein and crude fibre but a very significant rise in fats<br />

(14.3%).<br />

Discussion<br />

Macro and micro nutrient content<br />

In the two surveyed regions it was revealed that cowpeas are produced by majority of the<br />

farmers and consumed to a relatively large extent by all respondents, which provides an<br />

indication of cowpea importance in the two communities. Similarly, the survey revealed<br />

that cowpeas are prepared and consumed in a number of ways, such as relish for stiff<br />

porridge and rice, mixed and boiled with dehulled or un-dehulled maize and cowpea<br />

buns. In both regions, the cowpea buns were important as they were also a source of<br />

income in a number of households. The buns however, appear to have lower iron and<br />

calcium, especially those from Iringa region. The calcium, zinc and iron content of the<br />

cowpea buns were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma<br />

region had comparatively higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and<br />

iron (16.3mg/kg). This difference might emanatre from the method of preparation of<br />

cowpeas in Iringa in comparison to Dodoma. In Iringa the grains are dehulled and soaked<br />

for about three hours and then washed in flowing river water for about an hour. This<br />

procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />

these minerals are largely lost during washing.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

With regard to the deep frying of the cowpea buns, though some nutrients such as<br />

proteins and crude fibre were significantly reduced (from average of 22% to 19%<br />

protein), in the process fats were significantly increased (from average of 5% to 14%),<br />

while nitrogen free extracts and dry matter remained stable. The oil increase is attributed<br />

to the sunflower oil used to deep fry the buns. The farmers also cultivate sunflower for<br />

home use and sale. Greater oil intake is better as daily activities require adequate energy<br />

supply.<br />

Analysis of the developed cowpea varieties showed relatively high protein and fat in the<br />

levels of 24- 26% and 8%-11% respectively. Similar results have been observed by<br />

Horax et al., (2004) where they found cowpea seed contained 20-25% protein and was<br />

rich in essential and non-essential amino acids. Asante et al. (2006) were investigating on<br />

32 accessions of cowpea seeds, found protein and tannin contents ranging from 16.4-<br />

27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006)<br />

proximate analysis of various infested cowpea samples indicated increases in protein and<br />

crude fiber contents and decreases in moisture, ash, fats/oil and carbohydrates contents<br />

when compared with those of the uninfected samples. Percentage increases in protein and<br />

fiber contents ranged between 9.31-30.56%, and 1.24-15.11%, respectively while<br />

percentage decreases were, moisture (3.97-10.23%); ash (3.75-12.87%); fats/oil (1.43-<br />

10.00%); and carbohydrate (1.88-7.50%).<br />

Similarly the developed cowpeas were relatively high in calcium, iron, and zinc.<br />

However, considerable mineral variation was found among the analyzed cowpeas<br />

varieties. Similar study by Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea<br />

variety with the highest calcium concentration of 2096.0 μg/g and Zn was detected in only few<br />

varieties ranging from 1501.0 μg/g to 2071.0 μg/g. Analysis of variance of the samples<br />

showed significant differences (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

With regard to cowpea leaves, it was observed that the leaves are consumed by almost all<br />

in the two communities surveyed. The young leaves are the ones normally picked,<br />

usually the first three or four from the cowpea plant. While some chop the leaves into<br />

small pieces before cooking, others boil the whole leaves. The leaves seem to have a big<br />

contribution with regard to calcium, iron and zinc. According to Schippers (1997)<br />

African Indigenous Vegetables (AIVs) play a highly significant role in food security of<br />

the under-privileged in both urban and rural settings. AIVs have traditionally taken an<br />

important role in African diets, being used both as medicine and as a vegetable. Many of<br />

these crops are highly nutritious, easy and cheap to grow.<br />

Results indicate that, there was twice as much calcium in leaves than in cowpea grains<br />

and almost 40 times as much iron. Similar results were observed by Weinberger et al.<br />

(2004) where indigenous vegetables showed high mineral levels. Since the iron and zinc<br />

levels in cowpea leaves have shown to be significantly higher than the levels in cowpea<br />

grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />

contribute to alleviating micronutrient deficiencies from cheap sources especially in<br />

resource-poor families. However, it is also important to note that, vegetables that are<br />

common in a particular locality do not necessarily have values of iron contents that are<br />

comparable. For example, in a study by Weinberger et al. (2004) it was reported that<br />

cowpea leaves were identified and collected from four districts in Tanzania, their values<br />

were quite different varying from 179.0 mg in Kongwa, 66.0 mg in Singida, 77.5 mg in<br />

Muheza and 187.0 mg in Arumeru, per kg of edible portion. This is evident that the<br />

amount of minerals found in these vegetables does differ not only according to type of<br />

vegetable but also according to the place, location or district where they were obtained.<br />

This shows that the soil mineral content might have an influence in mineral uptake of the<br />

plants. This aspect can be further investigated.<br />

Adequacy of cowpea consumed in households<br />

As revealed in the results, the daily per capita consumption for the majority of the<br />

households surveyed ranged from 41 to 200 grams of cowpea, which means using the<br />

Atwater factors (4 kcal/g carbohydrates, 4 kcal/g protein and 4 kcal/g fats) an individual<br />

obtained about 135 to 658 kcal per day. Considering that a normal adult requires about a<br />

supply of 2500 kcal per day to perform moderate activities, the other amount of food<br />

eaten in conjunction with cowpeas such as rice or maize flour cannot fill the remaining<br />

gap. This shows that the amount of cowpeas consumed by most households does not<br />

satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed<br />

varieties, which are higher yielding and resistant to Alectra, they will probably be<br />

accepted by the communities and the amount per capita of cowpeas consumed should<br />

increase provided they continue to cultivate the same area of land.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Conclusion<br />

Cowpea grain and leaves are widely consumed in the two surveyed areas. The<br />

contribution of micro and macro nutrients is significant for both developed lines and local<br />

varieties but with leaves having greater mineral content than grain. Therefore awarenessraising<br />

within communities to consume more cowpea leaves is required. However the<br />

amount produced per household is low compared to requirements. Consequently cowpea<br />

intake per capita is low, and does not meet the RDA. However, since households in both<br />

areas still have ample land they should be encouraged to increase the area planted as well<br />

as planting higher yielding varieties in conjunction with preferred local varieties. This<br />

does however require further market investigation.<br />

Other important nutrients such as vitamins, amino acid profile and levels of<br />

antinutritional factors (tannins and phytates) should also be further investigated. The<br />

vitamins and amino acids influence in nutrition while the antinutritional factors influence<br />

on the bioavailability of the nutrients in the body.<br />

Acknowlegement<br />

The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong><br />

<strong>Foundation</strong>, who facilitated successful survey and laboratory analysis of macro and<br />

micronutrients.<br />

References<br />

Agazounon, C., Coulibaly, O. and Houndekon, V. (2004) Analysis of of cowpea<br />

processing techniques into ‘atta’ in Benin. Bulletin de la recherché Agronomique<br />

du Benin. No. 46, September 2004.<br />

Ahenkora, K. Adu Dapaah, H. K. and Agyeman, A. (1998) Selected nutritional<br />

components and sensory attributes of cowpea (Vigna unguiculata [L.]Walp)<br />

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<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

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