20.06.2013 Views

English - McKnight Foundation Collaborative Crop Research Program

English - McKnight Foundation Collaborative Crop Research Program

English - McKnight Foundation Collaborative Crop Research Program

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Edited by:<br />

A M Mbwaga ARI Uyole, Tanzania<br />

Joseph Hella Sokoine University of Agriculture, Tanzania<br />

J. Mligo Ilonga <strong>Research</strong> Institute, Tanzania<br />

V. Kabambe Bunda College University of Malawi<br />

September 2009<br />

1<br />

2009


Project Team<br />

<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Institution Name<br />

Tanzania<br />

Uyole Agricultural <strong>Research</strong> Institute, PO<br />

Box 400, Mbeya<br />

Ilonga Agricultural <strong>Research</strong> Institute, PO<br />

Box 33, Kilosa<br />

Sokoine University of Agriculture<br />

PO Box 3007, Morogoro<br />

Dodoma Rural District Council<br />

PO Box 1089, Dodoma<br />

Dr A M Mbwaga (Pathologist & Project Leader)<br />

~ Dr J K Mligo (Cowpea breeder)<br />

~ M. Mchomvu (Home Economics)<br />

~ Dr Joseph Hella (Agric. Economist)<br />

~ G. Martin (MSc Student)<br />

~ D. Muywanga (District Agricultural and Livestock<br />

Officer)<br />

~ Ms Mwamba (Subject Matter specialist)<br />

Singida Rural District Council ~ B. Manento (DALDO)<br />

~ L. Sakwera (<strong>Crop</strong>s specialist)<br />

Iringa Rural District Council<br />

PO Box 290<br />

INADES Formation, Tanzania<br />

PO Box 203, Dodoma<br />

Bunda College, University of Malawi, PO<br />

Box 219, Lilongwe<br />

Chitedze <strong>Research</strong> Station<br />

PO Box 158, Lilongwe<br />

Department of <strong>Crop</strong> Production, Ministry of<br />

Agriculture. PO Box 1035, Capital City,<br />

Lilongwe<br />

International Institute of Tropical<br />

Agriculture. C/O Lambourne Ltd, 26<br />

Dingwall Rd, Croydon CR9 3EE, UK<br />

~ P. Mphwewe (DALDO)<br />

~ Mrs Nyalusi (<strong>Crop</strong>s Specialist)<br />

A. Katunzi (Director & Training specialist)<br />

Malawi<br />

~ Dr V Kabambe (Weed Scientist, Senior Lecturer &<br />

project coordinator for Malawi)<br />

~ T Chilongo (Agricultural Economist)<br />

~ Dr J. Bokosi, (Plant Breeder)<br />

~ Dr A Mangwela (Food scientist)<br />

~ Ms Kazila. (MSc Student)<br />

~ William Henderson Harawa<br />

~ BSc Student in Agricultural Economics<br />

~ Mr E Mazuma (Pathologist & National Commodity Team<br />

Leader, Pulse <strong>Research</strong>)<br />

~ Ms C Mtambo. Entomologist and Chief <strong>Crop</strong> Protection<br />

Officer<br />

Nigeria<br />

Boukar Ousmane (Breeder) IITA Kano Nigeria<br />

2<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table of Contents<br />

Page<br />

Summary VI<br />

Introduction 2<br />

Project objectives 2<br />

Summary of research activities 2<br />

Objective 1. Development of high yielding A. vogelii resistant cowpea varieties 2<br />

Objective 2. Promotion of high yielding A. vogelii resistant cowpea varieties 13<br />

Implications of the research findings 15<br />

Annex 1 Tanzania Country Report 17<br />

Annex 2: Cowpea Utilization in Iringa and Dodoma Regions, Tanzania 23<br />

Annex3: Malawi Country Report 32<br />

3<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Summary:<br />

The parasitic weed Alectra vogelii is a wide spread constraint to cowpea production in semi-arid areas<br />

of Southern Africa, where the crop is an important source of protein for resource poor farmers. A<br />

project aiming to develop and promote Alectra resistant cowpea cultivars was initiated in Malawi and<br />

Tanzania. Farmer research groups have been established in cowpea growing areas of both countries<br />

and have been focusing on evaluation of cowpea lines for adaptation, yield, resistance to Alectra and<br />

processing and utilization of cowpea grain for three seasons now. Initial studies of farmers and market<br />

trader’s preferences in both countries Malawi and Tanzania indicate that large white to-cream seed<br />

types are most favored. Growers also need plant types that produce copious foliage for spinach and for<br />

preservation for consumption in the dry season. Cowpea germplasm collected from local institutions,<br />

farmers and the breeding program at the International Institute of Agriculture (IITA) Nigeria was<br />

evaluated. In Tanzania this season farmers have selected from five promising to three lines for their<br />

yield performance, Alectra resistance and earliness. Selected cowpea lines included IT99K-7-21-2-2-1,<br />

IT99K-573-1 and TZA 263. These lines are now being multiplied at Ilonga Agricultural <strong>Research</strong><br />

Institute for planting by more farmers on a large scale next season.In Malawi line IT99K-7-21-2-2-1<br />

was selected the best in terms of Alectra resistance, yield and grain size and is being earmarked for<br />

release. Parallel to this work some of these materials have been analyzed for macro and micro-nutrient<br />

content at Sokoine University of Agriculture Tanzania (see next section below). Post-graduate student<br />

projects on production, utilization and marketing of cowpea were completed in Tanzania last year, but<br />

there are still to be completed at Bunda College in Malawi.<br />

The processing of cowpea especially into grain was found to be a laborious work for women. Using<br />

tradition method of preparing - for example buns (Bagia) - a woman had to sleep late and to wake up<br />

around four in the morning ready to prepare Bagia. After introduction of new ways of preparing flour<br />

women are now it takes less than an hour to prepare Bagia, hence there is a lot of time saving. This can<br />

enable each member of the family to prepare Bagia for the children each morning. It has also been<br />

observed that dehuled grain can store for a long time without getting attacked by bruchids. The dehuled<br />

grain also cooks fast compared to grain. This is hoped to increased usage of cowpea grain at family<br />

level.<br />

Studies on micronutrients included also the frequency uptake of cowpea products by farmers in<br />

sampled villages of Iringa and Dodoma Tanzania Minerals of nutritional importance that were<br />

analyzed included iron, zinc and calcium; results indicated that, there was twice much calcium in leaves<br />

than in cowpea grains and almost 40 times as much iron. Since iron and zinc levels in cowpea leaves<br />

have shown to be significantly higher than the levels in cowpea grains, creating more awareness and<br />

encouraging the utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies<br />

from cheap sources especially from resource-poor families. The variety that was best with regards to<br />

all minerals was IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and<br />

fats content were IT97K499‐8 and IT99K‐721‐2‐2‐1.<br />

4<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction:<br />

Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural households in<br />

the drier and sub-humid region of Southern Africa, where diets tend to be overly reliant on starchy foods<br />

such as millet, sorghum, maize and cassava. The addition of even a small amount of cowpea ensures the<br />

nutritional balance of the diet and enhances the protein quality by the synergistic effect of high protein<br />

and high lysine from cowpea and high methionine and high energy from cereals. Hence cowpea grain is<br />

an inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />

particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />

harvest also it adds cash to the household after selling the grain and dried leaves. It has to be noted that<br />

cowpea is mainly a women crop.<br />

On-farm cowpea yields are extremely low, averaging 319 kg/ha in Tanzania and 388 kg/ha in Malawi.<br />

Use of late maturing cultivars, low plant density and insect damage are widely recognized as important<br />

constraints to improved cowpea production under on-farm conditions. Less well appreciated is the<br />

importance of the parasitic weed Alectra vogelii, which attaches itself to the roots of cowpea plants and<br />

interferes with the plants' ability to obtain water and nutrients. Recently-released improved cowpea<br />

cultivars that are earlier maturing and more tolerant to key insect pests and diseases are especially<br />

susceptible to Alectra attack, experiencing up to 50% yield reductions. A. vogelii is widespread from the<br />

Northern Province of South Africa, through Central Africa to Kenya and across West Africa to Mali. In<br />

Tanzania, A. vogelii is common in Mwanza, Shinyanga, Dodoma, Iringa, Mtwara and Ruvuma regions,<br />

while in Malawi, it is common in Lilongwe, Dowa and districts in central Malawi, the lower lying, drier<br />

areas of the southern region and the Blantyre/Shire Highlands<br />

Project objectives<br />

The general objective of the project is to improve cowpea productivity on A. vogelii-infested land in<br />

Malawi and Tanzania by introducing Alectra resistance into cowpea cultivars that are also early<br />

maturing, pest/disease tolerant and high yielding. The specific objectives are to develop high yielding A.<br />

vogelii resistant cowpea cultivars and then to promote them in both countries, Malawi and Tanzania.<br />

SUMMARY OF RESEARCH ACTIVITIES:<br />

Objective 1: Development of high yielding Alectra vogelii resistant cowpea varieties<br />

In year three of the project further studies have been undertaken to consolidate breeding through transfer<br />

of resistance to Alectra from the resistant lines (donor parents) into the commercial released varieties<br />

(recurrent parents) which are susceptible to Alectra by using a backcross method to improve the<br />

released varieties both in Malawi and Tanzania. Farmers groups established continued to be<br />

strengthened through training and participation in joint activities both within and outside their villages.<br />

Farmers in study villages continued to conduct on farm evaluation of the promising cowpea lines with<br />

commercially released varieties as checks. This year, 10 promising lines in Tanzania and five lines in<br />

Malawi were evaluated by farmers based on their own suitability criterion. In year three, project team<br />

continued to develop partnerships with farmer groups thus allowing continued on farm trials and<br />

availing useful data to research for making informed decisions in on-station breeding programmes.<br />

Through exchange visits and joint design of cowpea breeding programme, research collaboration<br />

between Malawi and Tanzania was increased and capacity was enhanced through farmer group training<br />

and graduate (BSc. and MSc.) students training. Details of activities undertaken on the outputs that the<br />

project had planned to address during the 2008/09 season (year 3) are presented in the individual county<br />

reports in the Annexes. A highlight of this work is as follows<br />

Output 1.1: Producer and consumer preference in cowpea identified<br />

The activities under this output were completed in year 1 and 2 and are found in annual report of 2008.<br />

5<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />

In Tanzania farmer groups have expanded from 6 to 8 and emphasis of the training was on the new<br />

farmer groups. Similarly two farmer groups were added in Malawi. The training given to the new farmer<br />

groups was on group formation, leadership and writing a group constitution. Training was also<br />

conducted on seed production using demonstration plots.<br />

Participatory Video shooting was one of the new activities introduced to farmers this season starting in<br />

Tanzania. Two farmer groups from Kikombo village (12farmers) in Dodoma and Mangalali village (14<br />

farmers) in Iringa region participated in the participatory video shooting training course. , The outcome<br />

has shown to be very effective in empowering farmers to document activities they are undertaking in the<br />

project. Their participation at CoP3 in Bagamoyo Tanzania was very evident and has created a<br />

motivation for them to undertake more of these documentation of project activities.<br />

Output 1.3: Cowpea lines resistant to A vogelii identified<br />

Assembling of cowpea germplasm:<br />

The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />

obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />

Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />

starting in the same year one. In Malawi the germplasm used came from IITA and from their national<br />

collection and Chitedzi <strong>Research</strong> Institute.<br />

On-station screening of cowpea cultivars for Alectra resistance<br />

In Tanzania, this activity started in 2007 growing season and the promising lines were tested to more<br />

Alectra infested sites. This year the trials were planted at three on-station locations which included<br />

Ismani, Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those<br />

from last season showing promising Alectra resistance and farmers had already selected some of them<br />

as the best lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288,<br />

TZA 263, IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207 and B301. VULI-2 and FAHARI<br />

were included as local checks. The materials were planted at a plot size of 3m x 4m, 4 rows per plot and<br />

replicated four times. Data recorded included; plant stand count at harvest, Alectra count per plot and<br />

yield per plot (kg/plot). Yield data was computed to yield in kg per ha.<br />

Generally weather condition was not very good for the crop, because rains started late and were erratic.<br />

Dodoma suffered the most for all the crops and this affected the crop establishment and Alectra<br />

infestation. From the test cowpea materials, only cowpea line IT97K-499-38 and B301 supported zero<br />

Alectra counts at three locations. (Table1). Other lines supported low Alectra plants as compared to the<br />

susceptible checks (Vuli-2 and Fahari) or TZA 263, and IT99K-573-2-1.The susceptible varieties<br />

(released commercial varieties) are being improved by incorporating resistance to Alectra by backcross<br />

method. At present the work is at backcross three progressing into backcross four, this will be followed<br />

by selfing then screening for resistance to Alectra.<br />

Table 1: Average infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />

locations at 12 weeks after planting 2009:<br />

Cowpea Entries Ismani Bihawana Hombolo Mean Comments<br />

FC<br />

Alectra<br />

count/sites<br />

IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of<br />

Alectra<br />

IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of<br />

Alectra<br />

IT97K-499-8 0.0 0.5 0.0 0.2<br />

IT97K-818-35 0.0 1.0 0.0 0.3<br />

6<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

IT97K-819-118 0.0 4.5 0.0 1.5<br />

IT97K-499-38 0.0 0.0 0.0 0.0<br />

IT89KD-288 0.0 26.3 0.0 8.8<br />

IT00K-1207 0.8 1.8 0.0 0.9<br />

IT96D-733 0.0 42.0 0.0 14.0<br />

B301 0.0 0.0 0.0 0.0<br />

TZA 263 0.0 1.5 0.0 0.5 Farmers selected<br />

for large grain<br />

Vuli 2 3.0 48.3 0.3 17.2 Commercial<br />

released varieties<br />

Fahari 7.5 34.3 1.0 14.3 Commercial<br />

released varieties<br />

Mean 0.87 12.73 0.15 7.58<br />

SE 0.41 2.98 -<br />

Range: 0-16 0-74 0-3<br />

In Malawi five of entries selected for further testing based on Alectra resistance, farmer preferences on<br />

grain characteristics and according to evaluations conducted in the last two years were planted at<br />

Chitedze and Bunda College. The trial’ design was complete block design, with 4 replicates. The trial<br />

plots had 5 rows, 4 m long and 0.75 m apart. Other traits evaluated included: grain yields and yield<br />

components (e.g. seed per pod,), days to flowering and maturity and disease incidences.<br />

Screening for Alectra resistance, entries IT 97K-825-15; IT99K-7-21-2-2-1 and IT 99K-494-6 recorded<br />

least number of Alectra counts (Table 2). Entries IT99K-494-6 (1369kg/ha), IT82E-16 (1786kg/ha) and<br />

Sudan 1(1827kg/ha) recorded high yield and the same entries showed larger number of seeds per pod<br />

than other entries<br />

Table 2: Alectra counts m -2 taken after flowering at all the trial sites in Malawi<br />

Entrycode & name Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />

IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />

IT97K-825-15 0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />

T99K-7-21-2-2-1 0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />

IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />

Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />

IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />

Farmers’ local 2.80 1.14 3.29 - 0.03 0.11 1.23<br />

Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot.<br />

With assistance from Village Extension Officers farmers were able to collect data and evaluate the<br />

materials based on their own set criterion.<br />

As discussed earlier, rainfall distribution was a major problem. It started late and erratic; hence there<br />

was very poor crop establishment especially in Iringa and Dodoma region. Singida received well<br />

distributed rainfall but there was a serious outbreak of cut worms on legumes in that region. The Alectra<br />

infestation was only reported on commercially released varieties and local cultivars, the other lines were<br />

observed free from the parasitic weed. The average highest cowpea yield was recorded from Singida<br />

region followed by Iringa and last Dodoma as presented in Table 3.<br />

Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania<br />

2009<br />

Cowpea line Farmer group managed to harvest from their plots of cowpea<br />

Mkungugu Iringa Kikombo Dodoma Msungua Singida<br />

IT99K-7-21-2-2-1 600 222 600<br />

IT99K 573-2-1 720 440 600<br />

IT97K 818-35 200 389 800<br />

IT89KD 288 400 333 200<br />

TZA 263 280 167 1200<br />

VULI-2 560 - 600<br />

FAHARI 800 - 760<br />

Village Local- 200 - 530<br />

Mean 470.0 310.2 661.3<br />

On-farm evaluation of cow pea lines (responses from farmers)<br />

Results of the on farm evaluation of selected cultivars was conducted in five participating villages;<br />

Msungua and IKhanoda (Singida region), Kikombo (Dodoma region), Mkungugu and Mangalali (Iringa<br />

region) based on the criterion they identified during the 2008 growing season i.e. resistance to Alectra,<br />

diseases/insects/pests, high yielding, large white to cream coloured seed, early maturity, production of<br />

many and tender leaves, drought resistance. These criteria were matched with five lines presented in<br />

Table 4.<br />

Table 4: Five best lines selected in 2008 evaluation session<br />

Lines Line description based on 2008 selection<br />

IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding, white<br />

coloured seed,<br />

IT 99K 573-1-1 Slightly late maturing, resistant to Alectra, average seed size, good seed colour,<br />

high yielding<br />

1T 97K 818-35 Late maturity, resistant to Alectra<br />

1T 89KD 288 Late maturity, high yielding, resistant to Alectra, normal seed colour,<br />

TZA 263 Late maturity, resistant to Alectra, good colour, large seeded<br />

Results indicated that line TZA263, followed by IT 99 K-7-21-2-2-1 and IT 99K-573-1-1 suited<br />

farmers’ social, economic and cultural interest. Based on these findings, lines TZA 263, IT 99K-7-21-2-<br />

2-1 and IT 99K-573-1-1 are to be increased for distribution to many farmers for further evaluation in<br />

2010 season. Initial process to establish descriptors for these lines for possible inclusion in the National<br />

Performance Trials (NPT) towards variety releases will be initiated.<br />

On farm evaluation in Malawi was conducted in Riviri, Bunda College, Mngwangwa, Mpokwa<br />

Khuvinda village, Mpokwa Chibisa village, and Lisasadzi. Seven entries involving four lines IT98K-<br />

8<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

503-1, IT97K-825-15, IT99K-7-21-2-2-1, IT99K-494-6, and two released varieties Sudan -1, IT82E-16<br />

and one farmers’ local cultivar were evaluated against five main criteria, which are; seed size, plant<br />

type, early maturity, insect resistance and disease resistance. Note that varieties Sudan -1 and IT82E-16<br />

were included for counterchecking with introduced lines. Preliminary description of the lines is<br />

presented in Table 5.<br />

Table 5: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />

Code Name Description<br />

5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra vogelii<br />

resistance<br />

37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />

preference<br />

9 IT99K-7-21-2-2- Best Alectra resistance in ’07, ‘07/08, good seed size, medium seed size,<br />

1<br />

good yield<br />

21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium seed size,<br />

low farmer preference<br />

53 Sudan -1 Released variety, good resistance to Alectra and good yielder in ‘07/08<br />

52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08. Poor<br />

resistance to A. vogelii. Well liked by farmers.<br />

54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />

Results of analysis show preference on the following order. Sudan 1 is most preferred followed by<br />

IT82E-16, IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6. Lines IT97K-825-15 followed by<br />

farmers’ local are less preferred as indicated above. Since Sudan 1 and IT82E-16 are already released,<br />

varieties IT99K-7-21-2-2-1, IT98K-503-1 and IT99K-494-6 may be considered for further genetic<br />

perfection on-station with a possibility of releasing them commercially starting with line IT99K-7-21-2-<br />

2-1.<br />

Geographic variability in host response to A. vogelii<br />

Studies undertaken in pot experiment at Long Ashton UK indicate that at a species level there<br />

are 3 strains of Alectra by host range:<br />

1. Alectra from Singida attaches and emerges on cowpea and groundnut but not on mung or common<br />

bean.<br />

2. Alectra from Bihawana and probably Ismani attaches and emerges on cowpea, groundnut, common<br />

bean but not on mung bean<br />

3. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and mung<br />

bean.<br />

Within cowpea, patterns of virulence were also observed to be evident: Alectra from all sites in both<br />

countries emerges on IT36E-16 and TZA 263 but not on B301.<br />

1. Alectra from Bunda and Kasungu emerges on ITK1207, IT97K 818-35, IT97K819-118 and IT<br />

99K7-21-2-2-1<br />

2. Alectra from Zomba emerges on ITK1207, IT97K 818-35, IT97K819-118 but not on IT 99K7-21-<br />

2-2-1 (this result for IT 99K7-21-2-2-1 same as in 2008 pot trial).<br />

3. Alectra from Singida and Ismani does not emerge on ITK1207, IT97K 818-35, IT97K819-118 and<br />

IT 99K7-21-2-2-1 (result for Singida same as in 2008 trial)<br />

4. Alectra from Bihawana emerges on IT 99K7-21-2-2-1 but not on ITK1207, IT97K 818-35 and<br />

IT97K819-118.<br />

In Tanzania it appears that Alectra from Singida has a narrower species host range and less virulent on<br />

the cowpea lines tested than Alectra from other sites. Bihawana Alectra provides a "universal" test for<br />

9<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

the three Tanzania sites. From this study the cowpeas that are resistant at Bihawana are also resistant at<br />

Singida and Ismani.<br />

In Malawi it appears that Alectra from Bunda and Kasungu are similar but different to Zomba.<br />

It has to be also noted that the Alectra samples we have from Malawi are different from the parasite<br />

collected further south near Blantyre as in trials some time back at Long Ashton this attacked B301<br />

(Mainjeni, 1999).<br />

It has been more confusing to find the result for IT81D-994 as this was resistant to all samples in 2008.<br />

There must be something went wrong with the samples delivered to Long Ashton this year.<br />

The most reliable method for characterization of these strains would be to go for using molecular<br />

markers.<br />

Reference:<br />

Mainjeni, C. E. (1999) The host range of Alectra vogelii Benth. From Malawi and resistance in common<br />

bean and cowpea. Msc. Thesis, University of Bath, UK. pp 83.<br />

Table 6: Response of 11 cowpea, one mung bean, one bambara groundnut and two groundnut lines to<br />

five accessions of A.vogelii.<br />

Alectra accession<br />

Zomba Malawi (ex cowpea) Bunda Malawi (ex groundnut) Kasungu Malawi (ex groundnut)<br />

Line Emerged No of<br />

reps<br />

Cowpea<br />

Un‐<br />

emerged<br />

Emerged No. of<br />

reps<br />

10<br />

Un‐<br />

emerged<br />

Emerged No. of<br />

reps<br />

2009<br />

Un‐<br />

emerged<br />

IT86E‐16 19.3 + 9.7 3 3 19.3 + 17.5 3 3 20.3 + 4.6 3 3<br />

B301 0 0 2 0 0 3 0 2 0 1<br />

TZA‐263 19 + 8.7 3 3 14.7 + 7.3 3 3 8.3 + 5.9 3 3<br />

IT00K‐1207 2.3 + 1.9 2 3 9 + 2.1 3 3 5.7 + 3.2 2 3<br />

IT81D‐994 1.3 + 0.7 2 3 1.3 + 0.9 2 3 0.3 + 0.3 1 3<br />

IT97K‐818‐35 1.3 + 1.3 1 3 2.7 + 1.8 2 3 3.3 + 2.8 2 2<br />

IT97K‐819‐118 0.7 + 0.7 1 3 3.3 + 2.4 2 3 1 + 0.6 2 2<br />

IT99K‐7‐21‐2‐2‐<br />

1<br />

Common bean<br />

0 0 3 0.7 + 0.2 2 3 0.7 + 0.3 2 3<br />

Kabalabala 1.7 + 1.7 1 NR 0 0 NR 2.7 + 2.2 2 NR<br />

Maluwa 4.3 + 2.2 2 3 3 + 0.8 3 3 4 + 2.5 3 3<br />

Masaka 1.0 + 0.6 2 3 5 + 4 2 3 3 + 1.7 2 3<br />

Mung bean<br />

Ex Tanzania 1.7 + 1.7 1 2 4 + 0.8 3 3 2.0 + 0.6 2 3<br />

Groundnut<br />

Chalimbana 0.7 + 0.7 1 NR 0.3 + 0.3 1 NR 1.3 + 1.3 1 NR<br />

CG7 2 0.5 + 0.4 1 NR 1.7 + 0.9 2 NR 2 + 1 2 NR<br />

1 Only one replicate pot established. 2 Only two replicate pots established, NR = not recorded yet.<br />

Table 6 continued.<br />

Singida Tanzania (ex cowpea)<br />

Alectra accession<br />

Bihawana Tanzania (ex cowpea) Ismani Tanzania (cowpea)<br />

Line Emerged No of Un‐ Emerged No. of Un‐ Emerged No. of Un‐<br />

Cowpea<br />

reps emerged<br />

reps emerged<br />

reps emerged<br />

IT86E‐16 13.3 +<br />

3.5<br />

3 3 9.3 + 6.8 3 3 3 + 0 3 3


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

B301 0 0 0 0 0 0 0 0 0<br />

TZA‐263 3.3 + 1.5 3 3 0.7 + 0.3 2 3 0.3 + 0.3 1 2<br />

IT00K‐1207 0 0 0 0 0 0 0 0 0<br />

IT81D‐994 0 0 1 2 2<br />

1 1 0.3 + 0.3 1<br />

IT97K‐818‐<br />

35<br />

0 0 2 0 0 1 0 0 1<br />

IT97K‐819‐<br />

118<br />

0 0 1 0 0 2 0 0 0<br />

IT99K‐7‐21‐<br />

2‐2‐1<br />

Common<br />

bean<br />

0 0 2<br />

0.7 + 0.3<br />

2<br />

2 0 0 1<br />

Kabalabala 0 0 NR 0 0 NR 0 0 NR<br />

Maluwa 0 0 2 1.3 + 0.3 3 3 0.3 + 0.3 1 2<br />

Masaka<br />

Mung bean<br />

0 0 3 2 + 1 3 3 0.3 + 0.3 1 2<br />

Ex Tanzania<br />

Groundnut<br />

0 0 0 0 0 0 0 0 0<br />

Chalimbana 0.7 + 0.7 1 NR 0 0 NR 0 0 NR<br />

CG7 0 0 NR 0 0 NR 0 0 NR<br />

NB: Mean number emerged stems per pot (+ S.E.) at 97 days after sowing. For entries with no emerged<br />

A. vogelii, the number of replicate pots with unemerged parasite attachments is shown. No standard<br />

errors are shown when there were less than three pots per cowpea/A. vogelii combination.<br />

Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />

Project activities are leading to increased capacity in cowpea research in Malawi and Tanzania and the<br />

strengthening of collaboration between institutions in both countries. Within the project, two researchers<br />

Abubakar Mzanda from Tanzania and Dr Vernon Kabambe from Malawi participated in a week long<br />

statistics training course offered by <strong>McKnight</strong> <strong>Foundation</strong> at Centre for Agricultural <strong>Research</strong><br />

Development (CARD)Bunda College University of Malawi June 15-22, 2009.<br />

• Two project staff – Drs J.P. Hella and V Kabamabe attended ASARECA review workshop on<br />

climate change. The workshop was held at Naivasha Kenya.<br />

• A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />

Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual<br />

Agricultural Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in<br />

AGREST conference series Vol. 8. (in press). The proceedings will be ready within and outside<br />

the country. Mr Gabriel Martin’s got financial support from the project for his MSc research work<br />

• Group dynamics training was conducted for all the farmers in the two participating villages in<br />

Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />

leadership and operation. This group strengthening included membership and responsibilities,<br />

constitution, networking and fund raising to sustain the groups.<br />

• During the season an undergraduate student (Ms Elida Kazira )undertook a study on cowpea time<br />

of planting x variety x density trial titled - Farmer Participatory Selection of Alectra Resistant<br />

Cowpea Varieties in Selected Districts of Malawi). The student has submitted her thesis for<br />

examination.<br />

• Participatory video workshop was held at INADES Dodoma, where two extension staff from the<br />

projects sites Dodoma and Iringa respectively participated and these also went back and trained<br />

farmers from the groups at Mangalali Iringa (14 farmers) and Kikombo in Dodoma (12 farmers)<br />

11<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 1.5: Project Monitored and evaluated<br />

To accomplish Activity 1.5.2 (Review progress against M & E criteria each year) monitoring and<br />

evaluation activities were conducted in Tanzania and Malawi as planned. The objective was to evaluate<br />

on going on-farm and on station cowpea project activities and crossing work in screen house.<br />

The monitoring and evaluation for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro)<br />

from 27 th to 30 th April, 2009 with participants including Ambonesigwe Mbwaga (Project Leader) ARI,<br />

Uyole Mbeya Tanzania, Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania; Vernon Kabambe (Weed<br />

specialist) Bunda College, Lilongwe, Malawi; James Bokosi (Breeder) Bunda College, Lilongwe,<br />

Malawi; Boukar Ousmane (Breeder,) IITA Nigeria. On-farm visits were done in regions of Iringa and<br />

Dodoma, whereas on-station visits were done at Ismani (Iringa region), Bihawana (Dodoma region and<br />

at Ilonga research institute (Morogoro region). Crossing for cowpea varieties to improve resistance to<br />

Alectra is being conducted.<br />

In Malawi, monitoring and evaluation was conducted from 15 th to 20 th March 2009. The team was<br />

composed of Drs Joseph Mligo (cowpea breeder), Ambonesigwe Mbwaga (Project team leader), Charlie<br />

Riches (CoP Liaison Officer) and Malawian counterparts comprising Dr Vernon Kabambe (Country<br />

project leader), Mosses Mamiliro (Breeder, and James Bokosi (Breeder). For On –farm activities 7<br />

cowpea cultivars of which 5 were experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-<br />

2-2-1, IT 97K 825-15, Bunda 1, IT98K503-1) one commercially released variety (IT 82E-16) were<br />

monitored in Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu district in<br />

Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA, Also seed<br />

multiplication of experimental cowpea lines such as TZA 263 (from Tanzania) and IT 81D-994. Seed<br />

increase also included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and<br />

IT82E-16.<br />

For on-station activities, the team visited Bunda college experimental site where seed multiplication of<br />

experimental cowpea lines which have shown Alectra resistance was being conducted. The<br />

performances of the materials were good and were at flowering stage in addition to seed multiplication<br />

activities at Bunda.<br />

Overall M&E team observed the following<br />

• Weather variability (especially drought) is an overriding problem for cowpea production. The<br />

team recommended the collaboration with the Tropical Legumes II project of IITA and try to<br />

incorporate both traits (Alectra resistance and drought tolerance) in the current breeding<br />

programme.<br />

• Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least<br />

preferred by the farmers. At all sites farmer’s variety was the latest, and it had not even<br />

flowered when all the other entries were maturing.<br />

• It was noted that only early maturing varieties were most preferred, forgetting the advantage of<br />

late maturing varieties for not having other traits such as prolonged availability of leaves as<br />

relish. This suggests that there is a need to evaluate the materials according to maturity groups.<br />

• The good production practices should be incorporated in the screening of the varieties against<br />

Alectra. Hence, there is need to expose the groups involved in the project to all these production<br />

practices.<br />

• There is a need to evaluate the materials according to maturity group: early, medium and late<br />

because farmers’ selections are dictated and influenced by time to maturity<br />

• The backcrossing programme at Ilonga will continue to backcross 6 by October 2010, while at<br />

Bunda will be at back cross 4. Request for extension of the project should be initiated early<br />

2010<br />

Overall the team noted good progress which are being made and high enthusiasm among stakeholders<br />

especially farmers who are conducting on-farm demonstrations<br />

12<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Objective 2: Promotion of high yielding A. vogelii resistant cowpea varieties<br />

With the continuation of the on-farm trials/demonstration component of the project efforts to increase<br />

awareness of the problem of A. vogelii and approaches to management continued in year 3. Work<br />

implemented included farmers field exchange visits, participation in national agricultural shows, training<br />

on preparation of different cowpea recipes and some market studies in Malawi<br />

Output 2.1: Farmer and extension awareness of .A. vogelii and use of resistant cultivars enhanced<br />

In the implementation of the above mentioned output, two activities were conducted in Tanzania, these are<br />

farmer field days and exchange visits and participation in 2009 National Agricultural show. With respect to<br />

exchange visit, twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu (Fahari research<br />

group) and later both visited Ismani On-station trials in Iringa. The objective of the farmer exchange visit<br />

was for farmers from Mangalali to share experiences with other farmers on principles of cowpea<br />

production, processing and utilization and to be acquainted with on station cowpea lines screening work. At<br />

the research station farmers participated in evaluation of 14 cowpea screening materials planted. Four<br />

cowpea farmers of the CCRP project from Singida rural district (two from Msungua and two from<br />

Ikhanoda) were sponsored by their district council and attended the National Agricultural Show between 1 st<br />

and 8 th August 2009 in Dodoma. Promising cowpea lines and associate processed products of cowpea from<br />

their group were displayed.<br />

In Malawi, farmers from Mngwangwa EPA, on their own accord, organized themselves and participated at<br />

a District Agriculture Fair held in the same EPA in June 2009. The farmers demonstrated a range of<br />

cowpea products based on the training made by the project and shared this with participants. In addition,<br />

the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science Department. Again,<br />

the focus of the exhibit was on utilization. The exhibits attracted a lot of attention from participants<br />

Output 2.2a: Nutritional status of farmers improved<br />

To accomplish output 2.2 above, activity 2.2.2: (Develop/modify/ demonstrate cowpea products) was<br />

undertaken. The primary objective was to promote the utilization of cowpea in Tanzania. Specifically was<br />

to train farmers on the processing of cowpea grains into dhal, preparation of cowpea dishes (snack bites<br />

from cowpea flour “bagia’); and to perform sensory evaluation and organoleptic tests. The training was<br />

conducted in eight villages namely Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />

Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The villages were<br />

purposively selected as a result of their involvement in a project on “Improvement of cowpea productivity<br />

on Alectra Vogelii infested land in Tanzania. A one day workshop was conducted in each project village<br />

and farmers were trained on improved cowpea technologies. The trainings were based on demonstrations,<br />

hands-on trials and printed materials (brochures for cowpea recipes). Farmers participated in the validation<br />

of five recipes for cowpea value addition. Sensory and organoleptic tests were carried out, where by<br />

farmers were required to rank and find out the most preferred types of ”bagia”. The five types of bagia<br />

were made using different recipes. The recipes were translated into Swahili language for better<br />

understanding by farmers.<br />

Table 7: Cowpea snack product recipes<br />

Type of<br />

Materials/ingredients<br />

Bagia<br />

1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />

2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />

3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and water<br />

4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and water<br />

5. Bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs, yeast and<br />

water<br />

13<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

In Malawi where farmers already have advanced knowledge on preparation of different<br />

cowpea/groundnut recipes, in areas where cowpea seed was provided, farmers were encouraged to use<br />

their cowpea to practice the different recipes which they learned from the project<br />

B: Cowpea Utilization in Iringa and Dodoma Regions<br />

Per capital consumption of 40‐200gms of cowpea grains were found to be 84% in Iringa and 94% in<br />

Dodoma, while per capital consumption for cowpea leaves (10‐500 grams) were 95% in Iringa and<br />

100% in Dodoma, respectively. More than 50% of households in Iringa and Dodoma consumed cowpea<br />

once to three times a week. The leaves are consumed fresh especially during wet season and in dried<br />

form during lean/dry season. Almost all farmers in the two regions consume cowpea leaves<br />

Macro‐ and micro‐ element analysis<br />

Analysis of the improved cowpea cultivars showed relatively higher protein levels of 24% to 26% and<br />

8% to 11% fat content. Similarly the improved cowpeas were relatively high in calcium, iron, and zinc.<br />

However, considerable mineral variation was found among the analyzed cowpeas varieties.<br />

Surprisingly, local cowpea cultivar had comparable high mineral calcium, zinc and iron concentrations<br />

compared with the improved varieties. This shows that apart from other merits that the improved<br />

cowpeas might have over local varieties such as higher yielding and resistance to certain diseases and<br />

pests, still nutritionally they are equally good. (See Annex 2)<br />

Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />

seed identified<br />

The seed availability is a big issue because commercial companies are not interested in producing seeds<br />

of low volume crops like sorghum, pearl millet bambara nuts and cowpea. To accomplish Activity 2.3.2<br />

{Train farmers to produce seed (QDS) for commercial use}, farmers were given basic training in<br />

community seed production covering four main topics namely Organization of seed production in<br />

Tanzania (L 1), Attributes of seed quality (L 2), Cowpea Seed Production (L 3) touching base on issues<br />

related to site selection for cowpea seed production, land preparation, choice of varieties to be planted<br />

according to days to maturity, and plant spacing depending on variety growth habit, crop husbandry<br />

practices such as pest management, rouging of off types, harvesting, seed conditioning, seed storage and<br />

seed sampling and testing for quality attributes. Table 8 present numbers of participants to on-farm seed<br />

production<br />

Table 8:. Participants to on-farm seed production for project participating farmers<br />

Region District Village Nane of<br />

No. of Topics Covered<br />

<strong>Research</strong> Group participants L1 L2 L3<br />

SINGIDA Singida Msungua Mshikamano 10 X X X<br />

rural Ikhanoda Mfwenji 20 X X X<br />

Nduu Matumaini 21 X X X<br />

DODOMA Dodoma Kikombo Ushirika 11 X X<br />

rural Mpunguzi Twende na<br />

wakati<br />

10 X X<br />

IRINGA Iringa Mkungugu Ari mpya 10 X X<br />

rural Mangalali Fahari 14 X X<br />

In Malawi, the project provided small packs of seed of 0.5 kg each to all project participating farmers at<br />

Mpokwa, Mngwangwa EPA (about 60 farmers at each EPA) and Kasungu. The objective was to create<br />

awareness on cowpea production. Subsequently, a follow-up training was conducted on pest<br />

management, to grow cowpea intensively (as sole crop) and to practice pest management and observe<br />

14<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

results. Farmers were monitored and they managed to get good crops and yields and were motivated to<br />

produce cowpeas. These farmers have been advised to store some of their seeds so that they can plant<br />

more cowpeas next season. They were also trained on principles of cowpea seed production during field<br />

days and visits.<br />

Output 2.4: Cowpea marketing opportunity identified<br />

In Malawi, cowpea market value chain workshops which brought representatives of cowpea producers,<br />

middlemen and traders were conducted in Lilongwe and Zomba in August 2009. The workshops were<br />

held to establish the linkages between cowpea producers (farmers) with the markets and to identify<br />

increased marketing opportunities. The workshop revealed that there are six big business traders who<br />

buy cowpea seeds (ADMARC, Rab Processors, Mulli Brothers, Agora, Grain Traders Association, and<br />

Transglobe) most of them paced emphasis on large seeded grain types as preferences.<br />

In Tanzania, the workshop was not held due to poor cowpea yields as a result of poor rainfall; hence<br />

farmers had nothing to offer to the traders. It is scheduled for May 2009 in Dodoma<br />

A paper - Gabriel, M. M.; Hella, J.P. and Mbwaga, A.M (2008) Assessment of Cowpea Marketing<br />

Efficiency: A case study of selected regions in Tanzania” will appear in the forthcoming AGREST<br />

conference proceedings Vol. 8 which is in press. Plan is underway to publish a paper in an international<br />

Journal for wider circulation. This is the first paper on cowpea marketing published in Tanzania<br />

Implication of the research findings<br />

During the past year, the project partners have built on work begun in year 1 and 2 to strengthen farmer<br />

groups, establish on farm trials and demonstrations, to take forward a cowpea-breeding programme,<br />

cowpea marketing stakeholders and to finalize marketing studies for Malawi. Crossing programme to<br />

incorporate resistance to Alectra into released commercial varieties has reached backcross 3 going in<br />

backcross 4 in Tanzania and backcross 2 in Malawi (due to delayed start in Malawi) These<br />

achievements will allow the project to move forward with next stage of the agreed work plan to<br />

undertake activities in the following ways;<br />

1. The project continues to support capacity building of farmer groups especially those new ones so<br />

that they are well informed about the aims of the project. Providing short term benefits for group<br />

membership should encourage continued participation in trials and other project activities.<br />

2. Crossing programme will continue up to backcross 6 by November 2010 in Tanzania, while in<br />

Malawi will be at backcross 4 because of late start.<br />

3. Wide scaling out identified Alectra resistant cowpea lines for Tanzania are three lines<br />

TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 and in Malawi are entries IT97K-825-15,<br />

IT99K-494-6 and IT99K-7-21-2-2-1<br />

4. Market studies have been completed in Tanzania and a stakeholders meeting will be conducted next<br />

financial year and in Malawi data is being analysed, a separate report will be prepared. Preliminary<br />

results were shared during the value chain workshops held in Lilongwe and Zomba respectively<br />

5. Farmer groups continued to be equipped with knowledge on QDS production and eventually. It is<br />

envisaged that QDS seed be bought by the project and then re-packed and farmers be given each<br />

member of the group give five new farmers of his choice to produce grain which can then be<br />

marketed by the group with aim to increase volume of grain production and hence increase grain for<br />

market at the same time popularize the varieties to more farmers.<br />

6. Since iron and zinc levels in cowpea leaves have shown to be significantly higher than the levels in<br />

cowpea grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />

15<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

contribute in alleviating micronutrient deficiencies from cheap sources especially for resource-poor<br />

families.<br />

7. The new line, which was best with regards to all minerals, was IT99K-7-21-2-2-1 while the best<br />

with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1. The later has<br />

been selected by farmers for its earliness in both countries Tanzania and Malawi (Item 3 above). It<br />

will be one of the potential candidate to be promoted for release in both countries Malawi and<br />

Tanzania<br />

16<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 1: Tanzania Annual Report<br />

Development and promotion of Alectra resistant cowpea cultivars for smallholder farmers in<br />

Malawi and Tanzania<br />

Edited by:<br />

Dr Ambonesigwe M Mbwaga: Uyole Agricultural <strong>Research</strong> Institute, Box 400 Mbeya<br />

Dr Joseph Mligo: Ilonga Agricultural <strong>Research</strong> Institute, Box 33 Kilosa, Morogoro<br />

Dr Joseph Hella: Sokoine University of Agriculture, Morogoro<br />

September 2009<br />

1<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Tanzania Project Team<br />

Uyole Agricultural <strong>Research</strong> Institute Dr A M Mbwaga (Pathologist & Project Leader)<br />

PO Box 400, Mbeya<br />

Ilonga Agricultural <strong>Research</strong> Institute Dr J K Mligo (Cowpea breeder)<br />

PO Box 33, Kilosa<br />

Dodoma Rural District Council<br />

D Muywanga (District Agricultural and Livestock<br />

PO Box 1089, Dodoma<br />

Officer)<br />

Ms Stella Mwamba (Subject Matter specialist)<br />

Singida Rural District Council N Mosha (DALDO)<br />

L Sakwera (<strong>Crop</strong>s specialist)<br />

Iringa Rural District Council<br />

P Mphwewe (<strong>Crop</strong> specialist)<br />

PO Box 290<br />

INADES Formation, Tanzania<br />

A. Katunzi (Director & Training specialist)<br />

PO Box 203, Dodoma<br />

TOSCI P.O BOX 1056, Morogoro Mwakitwange (Seed Technologist)<br />

Photo: Cowpea Seed production plot at Mpunguzi village in Dodoma, Tanzania 2009<br />

2<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction:<br />

The rainfall amount and distribution during the 2008/09 season was poor in most dry areas of Tanzania.<br />

Also there was an outbreak of cut warms at seedling stage especially in Singida, where farmers had to<br />

re-plant legumes more than twice until they ran out of seed. This has led to poor harvest of the crop. The<br />

two outputs 1.1 and 1.2 mentioned below were covered in year one and year 2.<br />

Output 1.1: Producer and consumer preferences for traits in cowpea identified<br />

This out put has been completed in year two<br />

Output 1.2: Capacity of cowpea farmers, especially women enhanced<br />

Three new more farmer groups were formed this season, one from each district of Singida, Dodoma and<br />

Iringa. These are from Nduu village (21 members) in Singida, Mpunguzi (10 members) in Dodoma and<br />

Ilambilole (8 members) in Iringa. Some preliminary training has been undertaken, mainly group<br />

formation, seed production and processing and utilization of cowpea.<br />

Output1.3.0: Cowpea lines resistant to A. vogelii identified<br />

1.3.1: To assemble cowpea germplasm:<br />

The collection of cowpea germplasm was conducted in year one, where cowpea accessions were<br />

obtained from IITA, Bihawana farmers Training Centre, National Pant Genetic Resource Centre and<br />

Ilonga Agricultural <strong>Research</strong> Station. These were evaluated for adaptability and Alectra resistance<br />

starting in the same year one.<br />

1.3.2 On-station screening of cowpea cultivars for Alectra resistance<br />

Introduction: Cowpea (Vigna unguiculata) is a crop of major importance to the nutrition of poor rural<br />

households in the drier and sub-humid regions of Eastern and Southern Africa, where diets tend to be<br />

overly reliant on starchy foods such as millet, sorghum, maize and cassava. Cowpea grain is an<br />

inexpensive, high quality source of protein and its vitamin-rich leaves are eaten as spinach. Women<br />

particularly value cowpeas, which help them to bridge the "hunger months" prior to the main cereal<br />

harvest, also it adds cash to the household after selling the grain, dried leaves and cookies like bagia<br />

(buns). Farmers grow local long duration cowpea cultivars, which produce low yield due to a number of<br />

reasons including Alectra infestation. In odder to improve the productivity of cowpeas in these areas,<br />

there was a need to introduce cowpea cultivars, which are high yielding, resistant to pests including<br />

Alectra and preferred by consumers both for market and for household use. The introduction of<br />

accessions was to asses for their resistance to Alectra. This activity started by this project in 2006/2007<br />

growing season and the promising lines were tested to more Alectra infested sites. These sites were<br />

Ismani in Iringa region; Bihawana Farmer Training Centre and Hombolo <strong>Research</strong> station both in<br />

Dodoma. For the second season 2007/2008 the cowpea lines were again planted at the same sites of<br />

Ismani, Bihawana FC and Hombolo to confirm their performance in terms of Alectra resistance and<br />

yield. Promising lines for Alectra resistance and yield were identified. In the third season 2008/2009, the<br />

promising lines were planted again at these Alectra hot spots for further confirmation of resistance to<br />

Alectra and for yield performance.<br />

Material and methods: The trial was planted at three on station locations which included Ismani,<br />

Bihawana Training Centre and Hombolo research station. Cowpea lines planted were those from last<br />

season showing promising Alectra resistance and farmers had already selected some of them as the best<br />

lines. These lines included IT99K-21-7-2-2-1, IT99K-573-1, IT97K-818-35, IT89KD-288, TZA 263,<br />

IT97K-819-118, IT97K-499-8, IT97K-499-38, IT00K-1207, B301 and VULI-2, FAHARI as local<br />

checks. The materials were planted at a plot size of 3x4m, 4 rows per plot and replicated four times.<br />

Data recorded were plant stand count at harvest, Alectra count per plot and yield per plot (g/plot). Yield<br />

data was computed to yield in kg per ha.<br />

3<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Results and discussions: Generally weather condition was not very good for the crop, because rains<br />

started late and erratic. Dodoma suffered the most for all the crops and this affected the crop<br />

establishment and Alectra infestation. The trial at Hombolo was mistakenly planted at a site where<br />

almost there was no Alectra infestation, only few Alectra plants were observed on susceptible cowpea<br />

checks Vuli-2 and Fahari. Looking at the susceptible checks high infestation of Alectra was observed at<br />

Bihawana Training Centre followed by Ismani. From the test cowpea materials only cowpea line<br />

IT97K-499-38 and B301 supported zero Alectra counts at both<br />

locations Ismani and Bihawana (Table 3.1). Other lines supported very low Alectra plants as compared<br />

to the susceptible checks Vuli-2 and Fahari.<br />

4<br />

2009<br />

Fig. 1 Cowpea field highly infested by Alectra at<br />

Bihawana Farmers training Centre Dodoma,<br />

2009<br />

Cowpea yield on average was observed highest at Hombolo followed by Bihawana FC and last was at<br />

Ismani. From the test cowpea lines, line IT99K-573-2-1 produced the highest average yield<br />

(735.6kg/ha) across sites followed by line IT97K-819-118 (647.3kg/ha). These lines also gave the<br />

highest yield from the test cowpea lines in 2008 season (Project Annual report 2008). Test line TZA 263<br />

was severely infected with a bacterial disease called bacterial blight especially at Ismani and Hombolo;<br />

in some of the plots almost all plants died due to the disease<br />

Fig. 2 Cowpea line TZA 263 attacked by a bacterial<br />

disease likely to be Bacterial blight (Xanthomonas<br />

vignicola)


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 1.Average Infestation of cowpea entries with Alectra vogelii (Alectra count/3x4m) at three<br />

locations at 12 weeks after planting 2009:<br />

Cowpea Entries Ismani Bihawana<br />

FC<br />

Hombolo Mean Alectra<br />

count across<br />

5<br />

sites<br />

Comments<br />

IT99K-7-21-2-2 0.0 3.8 0.0 1.3 Late emergence of Alectra<br />

IT99K-573-2-1 0.0 1.8 0.0 0.6 Late emergence of Alectra<br />

IT97K-499-8 0.0 0.5 0.0 0.2<br />

IT97K-818-35 0.0 1.0 0.0 0.3<br />

IT97K-819-118 0.0 4.5 0.0 1.5<br />

IT97K-499-38 0.0 0.0 0.0 0.0<br />

IT89KD-288 0.0 26.3 0.0 8.8<br />

IT00K-1207 0.8 1.8 0.0 0.9<br />

IT96D-733 0.0 42.0 0.0 14.0<br />

B301 0.0 0.0 0.0 0.0<br />

TZA 263 0.0 1.5 0.0 0.5 Farmers selected for large<br />

grain<br />

Vuli 2 3.0 48.3 0.3 17.2 Commercial released varieties<br />

Fahari 7.5 34.3 1.0 14.3 Commercial released varieties<br />

Mean 0.87 12.73 0.15<br />

SE 0.41 2.98 -<br />

Range: 0-16 0-74 0-3<br />

Table 2: Yield (kg/ha) of cowpea entries at Alectra vogelii hot spot at three locations, 2009<br />

Cowpea Entries Ismani Bihawana Hombolo mean yield Comments<br />

FC<br />

across sites<br />

IT99K-7-21-2-2 372.8 527.1 568.7 489.6 Farmers selected for<br />

earliness<br />

IT99K-573-2-1 309.4 927.1 970.4 735.6 Farmers selected for yield<br />

IT97K-499-8 410.2 643.7 753.9 602.6<br />

IT97K-818-35 432.3 525.0 608.2 521.8<br />

IT97K-819-118 590.8 677.1 674.0 647.3<br />

IT97K-499-38 362.5 409.6 604.2 458.8<br />

IT89KD-288 413.9 639.6 773.5 609.0<br />

IT00K-1207 262.9 545.8 515.2 441.3<br />

IT96D-733 404.6 302.1 514.3 407.0<br />

B301 164.8 952.1 789.2 635.4<br />

TZA 263 265.6 525.0 386.6 392.4 Large grain but severely<br />

infected by Bacterial blight<br />

disease.<br />

Vuli 2 212.7 354.1 867.3 478.0<br />

Fahari 209.8 554.2 745.6 503.2<br />

Mean 331.70 583.24 674.68<br />

SE 29.44 35.15 27.86<br />

Range: 92.5- 108.3- 236.6-<br />

791.7 1542.0 1198.0<br />

1.3.3a: On-farm evaluation of cowpea lines for Alectra resistance and yield:<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Introduction: Most farmers are growing long duration trailing type of cowpea, and mostly around their<br />

homestead. They are mainly for getting leaves and few pods. The grain yields are usually very low due<br />

to low yield potentials of the cultivars and farmers do not spray against insect pests at flowering and<br />

podding, hence, most of the pods are lost. In addition, the local varieties are very susceptible to Alectra<br />

vogelii. The introduction of early, Alectra resistant and high yielding cowpea varieties will improve the<br />

nutritional status and increase income to the households by selling extra produced grain. There is high<br />

potential market for cowpea and for improving household nutritional status.<br />

Material and Methods: Due to shortage of cowpea seeds, five villages were selected for evaluation of<br />

five best selected cowpea lines from last season for Alectra resistance, adaptation and yield.<br />

Commercially released varieties Vuli-2 and Fahari were added and their village local checks. The<br />

villages involved were Mangalali and Mkungugu (Iringa region), Kikombo in Dodoma and other two<br />

villages were Msungua and Ikhanoda in Singida rural district.<br />

The plots planted were 4m by 4m and the yields and Alectra counts were collected from the whole plot,<br />

which were separated by one meter. Farmers wanted to have larger plots, but we were limited with<br />

amount of seed. With their chairman and secretary of the group and with assistance from extension<br />

officers farmers were able to collect data and evaluate the materials according to their criterion<br />

Results and discussion: As discussed under section 3.1 rains distribution was a major problem. It was<br />

late and erratic; hence there was very poor crop establishment such that many of farmers just managed<br />

to get few cowpea pods especially in Dodoma and Iringa sites. The other problem observed in Singida<br />

was for unforeseen outbreak of cut worms, which occurred at germination of the crop. Some farmers<br />

had to replant more than two times until they ran out of seed. In Singida rainfall started on time and it<br />

was relatively evenly distributed as compared to Dodoma and Iringa rural districts. The Alectra<br />

infestation was only reported on commercial and local cultivars, the other lines were observed free from<br />

the weed pest<br />

The yields recorded on few farmers who managed to get a crop are as presented in Table 3.. The<br />

average highest cowpea yield obtained was from Singida rural followed by Iringa and last Dodoma rural<br />

district.<br />

Table 3: The yield (kg/ha) performance of the 5 cowpea lines tested at five Villages, in Tanzania 2009<br />

Cowpea line Farmer groups ir<br />

Mkungugu Kikombo Msungua<br />

Iringa Dodoma Singida<br />

IT99K-7-21-2-2-1 600 222 600<br />

IT99K 573-2-1 720 440 600<br />

IT97K 818-35 200 389 800<br />

IT89KD 288 400 333 200<br />

TZA 263 280 167 1200<br />

VULI-2 560 - 600<br />

FAHARI 800 - 760<br />

Village Local 200 - 530<br />

Mean 470.0 310.2 661.3<br />

1.3.3b: Farmer Participatory Cowpea Lines Evaluation<br />

Introductions: Cowpea (Vigna unguiculata (L) Walp), is one of the African indigenous most important<br />

leguminous crops. It is mostly grown in tropical countries for various uses and is a cheap source of<br />

vegetable protein. The crop is well adapted to relatively dry and stressful growing conditions of the<br />

marginal land, but produces excellent nutritive value (Singh et al., 1997). It is a drought tolerant crop<br />

and recently, there has been an increasing demand of cowpeas in the human diet, as well as for income<br />

generation. It thus provides solution for periodic hunger in semi-arid region of Tanzania<br />

6<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

This is the second season that the on farm evaluation of most promising lines of cowpea has been<br />

conducted in five villages namely; Msungua and Ikhanoda (Singida region), Kikombo (Dodoma region)<br />

and Mangalali and Mkungugu (Iringa region). Building from the 2008 evaluation, objectives of the 2009<br />

evaluation were as follow:<br />

~ To revisit the 2008 results<br />

~ To revisit evaluation criteria used in 2008<br />

~ To evaluate cowpea lines planted in 2009<br />

~ To make informed conclusion to guide for the subsequent on farm evaluation<br />

This brief presents the outcome evaluation of farmers’ trials in five villages in the above mentioned<br />

districts<br />

Method: The evaluation mission comprised Dr A.M. Mbwaga (Pathologist), Dr J.K. Mligo (Legume<br />

breeder) and Dr J.P. Hella (Agricultural Economist). As in 2008, the team participated in the on-farm<br />

evaluation in all villages for a period between 17 th and 24 th May 2009. In 2008 evaluation involved<br />

traversing with farmers in all five villages in each plot planted with a known variety of cowpea. Farmers<br />

were then requested to list, based on their own experience, the criteria which govern them to value and<br />

prefer particular varieties than another variety. Although main criteria varied across villages, but mostly<br />

hanged on high yield, early maturity, resistant to pests including Alectra, big sized seed and white to<br />

cream colour.<br />

Observations<br />

Criteria for selecting cowpea cultivar by villages in 2008<br />

Table 4 present the criterion used by farmers to rank different cowpea lines. The results were almost the<br />

similar in all villages. Good and more preferred lines are those associated with high yielding, early<br />

maturity, pest/disease (including Alectra) and drought resistant, large and white/cream seed colour.<br />

Table 4: Criteria for selecting different cowpea lines by village<br />

Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />

Early maturity High yielding High yielding High yielding High yielding<br />

Resistance to Alectra, Early maturity Drought Large seeds Large seeds<br />

diseases/insects/pests<br />

resistance<br />

High yielding varieties<br />

with many leaves<br />

Cream color Early maturing Early maturity Early maturing<br />

Brown color Alectra Resistant to Tolerant to Alectra<br />

resistant Alectra Alectra tolerance<br />

Big size- seeds Big size seed Cream Seed Reddish seed Drought<br />

color<br />

colour<br />

resistance<br />

Large seeds Plenty green<br />

leaves<br />

Cowpea lines preferred by farmers in target villages 2008<br />

Based on criteria highlighted in Table 4 above, in May 2008, farmers evaluated 14 lines including the<br />

commercial released varieties i.e. Vuli-2 and Fahari and locally grown cultivars named by the respective<br />

village name. List of cowpea lines evaluated in 2008 is presented in Table 5 below:<br />

Table 5: List of cowpeas line planted and evaluated in 2008 season<br />

No Lines/cultivar<br />

1 IT 99K-7-21-2-2-1<br />

2 IT 99K-573-1-1<br />

3 IT 97K-499-8<br />

4 IT 97K-818-35<br />

5 IT 97K-819-118<br />

6 IT 97K-499-38<br />

7<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

7 IT 89KD-288<br />

8 IT 00K-1207<br />

9 IT96D-733<br />

10 B 301<br />

11 TZA 263<br />

12 Vuli-2<br />

13 Fahari<br />

14 Farmer’s variety<br />

(Msungua/Ikhanoda/Kikombo/Makungugu/Mangalali)<br />

Table 6: Cowpea lines selected by each participating farmer Group in 2008 based on criteria in<br />

Table 4<br />

# Msungua Ikhanoda Kikombo Mkungugu Mangalali<br />

1 IT99K-7-21-2-2 TZ A263 IT 00K-1207 TZA 263 IT 99K-7-21-2-2<br />

2 IT97K-499-8 IT99K-7-21-2-2 IT97K-818-35 IT 99K-573-1-1 IT 97K-499-8<br />

3 IT99K-573-1-1 IT97K-818-35 IT97K-499-38 IT 97K-499-8 IT 97K-819-118<br />

4 IT00K-1207 IT97K-499-38 IT96D-733 IT 89KD-288 TZA 263<br />

5 IT97K-818-35 IT99K-573-1-1 B301, IT 97K-499-38 IT 99K-573-1-1<br />

6 IT97K-499-38 IT97K-499-8 T99K-573-1-1, IT 97K-818-35 IT 97K-499-38<br />

Ranking for 2009<br />

At the beginning of the season, five out of 14 lines planted in 2008 were selected for planting at<br />

farmers’ plots. The lines selected for the current year was based in farmers’ own expression as indicated<br />

in Table 6 above. Lines presented for further evaluation and reasons advanced by farmers are as<br />

presented in table 7 below<br />

Table 7: Cowpea lines selected for on-farm experiment in 2009<br />

# Lines Varieties<br />

1 IT 99K 7-21-2-2 average seed size, early maturity, resistant to Alectra, high yielding,<br />

white cream coloured seed,<br />

2 IT 99K 573-1 Slightly late maturity, resistant to Alectra, average seed size, white<br />

cream seed colour, high yielding<br />

3 1T 97K 818-35 Late maturity, resistant to Alectra<br />

4 1T 89KD 288 Late maturity, high yielding, resistant to Alectra, white cream seed<br />

colour,<br />

5 TZA 263 Late maturity, resistant to Alectra, reddish brown colour, large seeded<br />

Msungua -Village, Singida<br />

In Msungua village, 16 farmers participated at the on-farm evaluation. Results are as presented in Table<br />

8 below. Line IT 99K 7-21-2-2-1 was the most preferred and IT89KD288 was the least preferred line.<br />

Table 8: Pair-wise ranking for Msungua village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 1 1 1 1 4 1<br />

IT 99K 573-1-1 2 2 2 3 2<br />

1T 89KD 288 4 5 0 5<br />

1T 97K 818-35 5 1 4<br />

TZA 263 2 3<br />

8<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Ikhanoda-Village, Singida<br />

At Ikhanoda village, 12 farmers participated at the on-farm evaluation and results are as presented in<br />

Table 9 below. Line TZA 263 was the most preferred while IT 89KD 288 was the least preferred line.<br />

Table 9: Pair-wise ranking for Ikhanoda village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2 1 1 1 5 3 2<br />

IT 99K 573-1-1 2 2 5 2 3<br />

1T 97K 818-35 4 5 1 4<br />

1T 89KD 288 5 0 5<br />

TZA 263 4 1<br />

Kikombo village, Dodoma<br />

In Kikombo village, 6 farmers participated at the on-farm evaluation and results are as presented in<br />

Table 10 below. Line TZA 263 was the most preferred but IT 89KD 288 was the least preferred line.<br />

Table 10: Pair-wise ranking for Kikombo village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 3 2<br />

IT 99K 573-1 1 2 3<br />

1T 97K 818-35 1 2 1 4<br />

1T 89KD 288 1 2 3 0 5<br />

TZA 263 5 5 5 5 4 1<br />

Mkungugu –Ismani, Iringa<br />

In Mkungugu village, 10 farmers belonging to Ari Mpya farmer research group participated at the onfarm<br />

evaluation. Results are as presented in Table 11 below. Line TZA 263 was the most preferred and<br />

IT 97K 818-35 was the least preferred line.<br />

Table 11: Pair-wise ranking for Mkungugu village<br />

Lines 1 2 3 4 5 Result Rank<br />

IT 99K 7-21-2-2-1 1 1 1 5 3 2<br />

IT 99K 573-1 2 2 5 2 3<br />

1T 97K 818-35 4 5 0 5<br />

1T 89KD 288 5 1 4<br />

TZA 263<br />

Mangalali - Iringa<br />

5 1<br />

For unforeseen reasons it was not possible to evaluate the materials on day the team visited Mangalali<br />

village. When the farmers from Managalali visited Ismani research station, they were given opportunity<br />

to evaluate replicated trial planted with 14 lines including those planted at their own plots at Mangalali.<br />

The results are as presented in Table 12 below, where TZA 263 was ranked the best line followed by<br />

IT99K-7-21-2-2.<br />

Table 12: Ranking of the cowpea lines by Mangalali farmers at Ismani research Station 2009<br />

No Cowpea line Number of Number of Ranking of the Comment<br />

participants farmers selected cowpea line<br />

1 TZA-263 26 24 1 Each farmer<br />

2 IT99K-7-21-2-2-1 20 2<br />

was given a<br />

3 B 301 9 3<br />

chance of<br />

4 ITOOK-1207 8 4<br />

selecting only<br />

5<br />

6<br />

IT96D-733<br />

IT89KD-288<br />

8<br />

6<br />

4<br />

5<br />

best three<br />

cowpea lines<br />

7 IT99K-573-1 3 6<br />

9<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Conclusion<br />

The main objective of the field visitation was to evaluate farmer managed experiments in all study<br />

villages. Based on the approach and criteria agreed by the farmers, final rank on 5 lines introduced to<br />

them is presented in Table 10. The most preferred line is TZA, 263, followed by IT 99 K-7-21-2-2-1<br />

and IT 99K-573-1-1. Position four and five were contested between line IT 89KD-288 and IT 97K 818<br />

which were scored by two villages in the same positions. After revisiting some inherent characteristics<br />

of the lines as scored by same farmers in 2008, line IT 89KD-288 was relatively batter on same traits<br />

such as early maturity and leave sizes which are lacking in IT 97K-818. Thus IT 89KD-288 was ranked<br />

4 th while IT 97K-818 was ranked 5 th . as indicated in Table 10<br />

Table 10: Farmer preference with respect to 5 introduced cowpea LINES<br />

Villages Rank in order of preference<br />

1 st 2 nd 3 rd 4 th 5 th<br />

Msungua IT 99K 7-21-2-2-1 IT 99K 573-1-1 TZA 263 1T 97K 818-35 IT 89KD 288<br />

Ikanoda TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />

Kikombo TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 97K 818-35 IT 89KD 288<br />

Mkungugu TZA 263 IT 99K 7-21-2-2-1 IT 99K 573-1-1 IT 89KD 288 IT 97K 818-35<br />

Mangalali TZA 263 IT99K-7-21-2-2-1 1T89KD-288 IT99K-573-1 1T 97K 818-35<br />

Rank (1) TZA 263<br />

(2)<br />

IT 99K 7-21-2-2-1<br />

(3)<br />

IT 99K 573-1-1<br />

(4) IT 89KD 288<br />

(5) IT 97K 818-35<br />

Based on these findings, lines TZA 263, IT 99K-7-21-2-2-1 and IT 99K-573-1-1 are to be increased for<br />

distribution to many farmers for more evaluation. For these lines, initial process to establish descriptors<br />

for these lines for possible inclusion in the National Performance Trials (NPT) towards variety release<br />

should be started.<br />

1.3.4 Targeted crosses<br />

Tanzania cowpea crossing program<br />

In 2007 report 130 cowpea accessions were screened for Alectra resistance. 10 accessions could not<br />

support emergence of Alectra. The identified accessions that could not support emergence of Alectra<br />

plants were classified as resistant to Alectra. These were crossed to the desirable parents (the released<br />

varieties which are: Fahari, Tumaini, Vuli-1 and Vuli-2) with the aim of transferring the Alectra<br />

resistance into these released varieties. Since resistance to Alectra is conditioned by a single gene, a<br />

backcross method was used to transfer of resistance. At present the screening (September, 2009) is at<br />

backcross three plants. The resistant plants will be identified while they are still flowering, thus the<br />

resistant backcross three plants will be backcrossed to the desirable parents to obtain backcross four<br />

seed which will be planted and selfed then followed by screening for resistance to Alectra. This process<br />

will continue up to backcross six at which it is expected a single chromosome segment carrying the<br />

desired gene will have been incorporated into the desired parents. Planting of backcross four seed will<br />

be conducted in early November 2009. The selfed backcross four seed will be planted in late February<br />

2010 for screening again for resistance to Alectra and resistant plants backcrossed to the desired parent<br />

to produce backcross five seed. Backcross five seed will be planted for selfing in early May 2010 and<br />

the selfed backcross five seed will be planted for screening for Alectra resistance in early august 2010.<br />

Resistant plants will be backcrossed to desirable parent in October 2010 to produce backcross six seed.<br />

Backcross six seed will be selfed ready for evaluation for Alectra resistance and yield on Alectra<br />

infested fields in season 20010/011 with the expectation that the project will be extended.<br />

10<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 14: Backcross three crosses in the screen house at ARI-Ilonga by September 2009<br />

Cross number Pedigree<br />

17 (B301 x Vuli-1) x Vuli-1<br />

30 (IT 97K-499-8 x Vuli-2) x Vuli-2<br />

38 (Tumaini x IT96D-733) x Tumaini<br />

48 (ITK-499-8 x Tumaini) x Tumaini<br />

Output 1.4: Collaboration in cowpea research increased in Southern Africa<br />

Activity 1.4.1: Across site evaluation (PL to visit programme in Malawi)<br />

A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra project activities<br />

The team was composite of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />

leader and Charlie Riches, CoP liaison officer and met by the Malawian project team that was<br />

composite of Dr Vernon Kabambe country project leader, Mosses Mamiliro (breeder) ,and James<br />

Bokasi (breeder)<br />

The evaluation tour for Tanzania was done in three regions (Iringa, Dodoma, and Morogoro) from 27 th<br />

to 30 th April, 2009. The tour involved visiting seed multiplication, on-farm, on-station, and crossing<br />

programme. The team comprised of the following:<br />

Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />

Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />

Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />

James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />

Boukar Ousmane (Breeder,) IITA Nigeria<br />

Activity 1.4.2: Student projects<br />

For Tanzania an MSC student working on a project “Assessment of cowpea marketing efficiency; A<br />

case of selected regions of Tanzania” was completed and the student graduated November 2008.<br />

In Malawi an MSc student working on a project “Farmer Participatory Selection of Alectra Resistant<br />

Cowpea Varieties in Selected Districts of Malawi”. The thesis has been submitted to examiners for<br />

review. In addition in Malawi an BSc student is undertaking a study on “Cowpea variety x time of<br />

planting x density”<br />

Activity 1.4.3: Paricipation in CCRP "community of practie<br />

Three researchers, one extensionist and one farmer from the project attended CoP2 in Maputo<br />

Mozambique in October 2008. In CoP3 in Bagamoyo Tanzania 4 researchers and 4farmers from project<br />

sites attended<br />

Activity 1.4.4: End of project stakeholder’s workshop (location to be determined later)<br />

Output 1.5: Project monitored and evaluated<br />

Activity 1.5.1. Establish project level M & E criteria<br />

(done during partners meeting in Mbeya at the inception of the project)<br />

Activity1.5.2. Review progress against M & E criteria each year<br />

Introduction: A trip to Malawi was made from 15 th to 20 th March 2009 to evaluate Cowpea/Alectra<br />

project activities.<br />

The team was composed of Drs Joseph Mligo cowpea breeder, Ambonesigwe Mbwaga Project team<br />

leader and Charlie Riches( CoP- liaison officer) and met by the Malawian project team that was<br />

composed of Dr Vernon Kabambe country project leader, Mosses Mamiliro( breeder), and James<br />

Bokosi (breeder).<br />

The objective of the visit were<br />

To evaluate on going on-farm cowpea activities, on station project activities and crossing work in screen<br />

house<br />

11<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

On –farm activities: On farm activity included evaluation of 7 cowpea cultivars of which 5 were<br />

experimental lines for Alectra resistance (IT99K-494-6, IT99K-7-21-2-2-1, IT 97K 825-15, Bunda 1,<br />

IT98K503-1) one commercially released variety (IT 82E-16) and a farmer’s variety at each project site.<br />

The project sites included Lilongwe district in Ngwangwa Extension Planning Area (EPA), Kasungu<br />

district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi EPA.<br />

Lilongwe district<br />

Ngwangwa Extension Planning Area (EPA)<br />

a. Mazila village: A farmer research group in Ngwangwa was composited of 30 members but due to<br />

shortage of cowpea seed only four farmers planted the 7 cultivars. The general performance of the<br />

cultivars was very poor which resulted in poor pod setting. The possible reasons are: the soil fertility<br />

looked very poor, also it appeared to have had high insect incidences.<br />

The Alectra infestation was very high especially on the released variety (IT 82E-16) and Bunda 1. Less<br />

infestation was observed on IT97K-825-15, IT99K-494-6 and IT99K-7-21-2-2-1.<br />

b. Mpondela: Here the group was able to visit field of one farmer. There was good performance of all<br />

the entries planted and there was no Alectra observed in all of the seven entries.<br />

Kasungu District<br />

Lisasi EPA<br />

Sikondeyani village: Five farmers planted the trial. The trial performance was very good compared to<br />

those of Ngwangwa EPA. This may be due to good soils and relatively good rain Although nearby fields<br />

planted with cowpea had very high Alectra infestation but the infestation levels in the trials was<br />

relatively low and late emergency for this reason the grain yield was not much affected. Farmers<br />

selected IT82E-16 and Bunda 1 as the best varieties in terms of yield and earliness.<br />

Zomba district<br />

Mpokwa EPA<br />

Khuvinda village: There was good performance of the cowpea lines with very low Alectra infestation.<br />

Out of the seven entries, two entries (IT82E-16 and Bunda 1) were selected by farmers to be the best<br />

due to their earliness and good pod setting. Although the released variety IT82E-16 is a short duration<br />

variety, farmers were not aware of such type of the variety. This emphasizes the need to carry out more<br />

demonstrations to create awareness to more farmers.<br />

Balaka district:<br />

Rivirivi EPA: This is the only area where farmers were not organized in a formal farmer research group.<br />

The demonstrations were very good and planted along the road side for everybody to see. Alectra<br />

infestation was very low. As in other villages, here farmers also selected the early maturing varieties as<br />

the best cultivars.<br />

Bunda College: Activities here included, seed multiplication of experimental cowpea lines which have<br />

shown Alectra resistance. These included TZA 263 (from Tanzania) and IT 81D-994. Seed increase also<br />

included IT98K-503-1, IT99K-7-21-2-2-1, IT99K-494-6, IT97K-825-15, Sudan 1 and IT82E-16. The<br />

performances of the materials were good and were at flowering stage. The materials for crossing were<br />

planted in screen house and were at vegetative stage. Some of them had supported Alectra emergence<br />

already, which were recommended to be removed out of the experiment because they had shown<br />

susceptibility to Alectra.<br />

Lessons learnt:<br />

12<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Although IT97K-825-15 had the least Alectra infestation at all on-farm sites it was the least preferred by<br />

the farmers. At all sites farmer’s variety was the latest, and it had not even flowered when all the other<br />

entries were maturing.<br />

It was noted that only early maturing varieties were most preferred, forgetting the advantage of late<br />

maturing varieties for having other traits such as prolonged availability of leaves as relish. This suggests<br />

that there is a need to evaluate the materials according to maturity groups.<br />

Cowpea and pigeon pea as the main source of vegetable protein in the project target sites; it was<br />

observed that early maturity of the cowpea bridge the hunger period of February/March, while waiting<br />

for the pigeon pea to mature late in the season.<br />

Wrap up:<br />

- Released varieties showed better yields than test lines<br />

- Early maturing varieties save as source of food in hunger periods<br />

- Seed system for cowpea is very poor<br />

- More cowpea seed should be sent to Balaka and Kasungu districts as there is high demand for seed<br />

of the improved varieties.<br />

- Farmers in Rivirivi recommended forming farmer research groups for better access by researchers<br />

and extension staff as compared to individual isolated farmers<br />

TANZANIA<br />

27 th -30 th April, 2009<br />

Introduction: The monitoring and evaluation tour for Tanzania was done in three regions (Iringa,<br />

Dodoma, and Morogoro) from 27 th to 30 th April, 2009. The tour involved visiting seed multiplication,<br />

on-farm, on-station, and crossing activities. The objective was to monitor progress in implementing<br />

agreed strategies and action plans for the project. This report gives information on sites/farmers/stations<br />

visited, varieties evaluated, team observations and way forward.<br />

The monitoring and evaluation team comprised the following:<br />

Ambonesigwe Mbwaga (Project Leader) ARI, Uyole Mbeya Tanzania<br />

Joseph Mligo (Breeder) ARI, Ilonga Kilosa Tanzania<br />

Vernon Kabambe (Striga specialist) Bunda College, Lilongwe, Malawi<br />

James Bokosi (Breeder) Bunda College, Lilongwe, Malawi<br />

Boukar Ousmane (Breeder,) IITA Nigeria<br />

On-farm visits: A number of farmers groups were visited in the two regions (Iringa, and Dodoma). In<br />

general farmers were required to plant five IITA lines and two locally released varieties (Fahari and<br />

Vuli-2). The two released varieties showed to be more adapted and performed consistently well across<br />

locations despite the prevalence of drought in many locations. The major problem is that they are both<br />

susceptible to Alectra. In a participatory variety trial conducted at Mpunguzi, Dodoma region, farmers<br />

selected IT 00K-126-3 as the best entry despite being susceptible to Alectra. Many of the farmers<br />

visited showed dedication to running the on-farm trials.<br />

On-station visits: The first on-station trial visited was at Ismani. The trial had 14 entries replicated four<br />

times. Each plot had five rows of four meter length. Although drought had an impact the performance<br />

of the entries was far better than the farmers’ fields. Alectra pressure was not as high as the previous<br />

year. This could have been due to the prolonged dry spell during the growing season. Vuli-2 and Fahari<br />

performed very well despite sustaining some Alectra plants. Except for the two check entries the rest<br />

had no Alectra emergence. The second trial was at Bihawana (Bihawana farmers training centre). The<br />

entries were similar to those planted at Ismani but four extra entries. Rainfall distribution was not good.<br />

The site received a mere 250 mm (normal 330 -488 mm) of rainfall between February (151.3mm) and<br />

March (98.8mm) and poorly distributed. This site had the heaviest infestation of Alectra and is good for<br />

13<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

screening. High insect population was observed at this site as compared to the other sites. Vuli-2<br />

looked good but not outstanding. Entry IT 573-1 doing well and B301 though small seeded. <strong>Crop</strong><br />

management was very good despite drought. IT99K 573-1 and B301had no Alectra in all four<br />

replicates. Two entries, IT99K-7-21-2-2-1 and TZA 263 had only one Alectra plant in one replication.<br />

The third on-station trial was at Hombolo. The entries were same as those planted at Ismani and<br />

Bihawana. The planting date was 16 th January, 2009 and germination occurred on 4 th February, 2009<br />

because of lack of rain after planting. However the Alectra pressure was not high. Field management<br />

was good and the entries performed well.<br />

Seed production: A major objective of this activity was to teach farmers on how to produce quality<br />

seed. The first seed multiplication activity visited in Iringa was at a community plot known as. Three<br />

varieties were planted (Fahari, Vuli-2, and local cultivar). The varieties were planted on 25 th February,<br />

2009 and entries were just starting to flower. The isolation distance was 2.5 meters and crop looked<br />

very clean. Dimethoate was used to control aphids. The soil was quite sandy. Farmers claim late<br />

planting was due to late land preparation because other farmers contributed late for hiring oxen used for<br />

land preparation. There was poor effort in labelling entries.<br />

The second visit was at Mpunguzi, Dodoma region. Two farmers planted one variety (Fahari) on 2 nd<br />

February, 2009. One farmer had a poor field but the other one managed the crop well (one acre) and will<br />

harvest a good yield despite the drought.<br />

Cowpea crossing programme at ARI Ilonga<br />

The visit to see crossing programme was only to Ilonga research institute where the crossing for cowpea<br />

varieties to improve resistance to Alectra is being conducted. This station is in Kilosa district. The<br />

research mandate includes other crops such as maize, sorghum, soybean, sunflower, cotton, pearl millet<br />

besides cowpea. The crossings were done in a screen house and involved commercially released<br />

varieties as recurrent parents in a backcrossing program such as Tumani, Fahari, ,Vuli-1 and Vuli-2.<br />

Aphid infestation is a major problem in the screen house. Backcross 3 seed had been harvested ready for<br />

planting to make backcross 4.<br />

WAY FORWARD/RECOMMENDATIONS:<br />

Drought (rainfall, poor distribution and low amounts) has been an overriding problem this season. We<br />

commend the collaboration the project is undertaking along with the tropical legumes II project of IITA.<br />

The project should therefore incorporate both traits (Alectra resistance and Drought tolerance). An<br />

analysis of medium term climatic data (20-25 years) would be important to determine the frequency of<br />

rainfall events (e.g. start dates, dry spells and rainfall amounts). This can help to determine the<br />

important drought traits in plant (e.g. earliness or resilience). The good production practices should be<br />

incorporated in the screening of the varieties against Alectra. Hence, there is need to expose the groups<br />

involved in the project to all these production practices. There is a need to evaluate the materials<br />

according to maturity group early, medium and late because farmers’ selections are dictated and<br />

influenced by time to maturity. The experiences observed from this project and TLII project indicate<br />

that the overriding preference trait is seed size; large seeded types are more liked than small seeded<br />

types. Thus this should be taken care in our project. The backcrossing programme at Ilonga will<br />

continue to backcross 6 by October 2010, while at Bunda will be at back cross 4. Request for extension<br />

Objective 2: Promotion of high yielding A. vogelii resistant cowpea cultivars<br />

Output 2.1 Farmer and extension awareness of Alectra vogelii and use of resistant cultivars<br />

Activity 2.1.1: Develop leaflets on crop management and ultilization of cowpea, pre-test and print<br />

A draft leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These<br />

were produced in Kiswahili for better understanding by farmers. It emphasized on the different ways of<br />

cowpea utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be<br />

converted into other value-added products such as dhal, stew and snack products like “bagia”. This<br />

14<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

leaflet has been printed out for further dissemination of the technologies. The leaflet on crop<br />

management is to be produced next cropping season<br />

Activity 2.1.2a: Farmer exchange visits and field days across project sites<br />

Farmer exchange visit involved twelve farmers from Mangalali (Ari Mpya Group) visited Mkungugu<br />

(Fahari Group) farmer <strong>Research</strong> Group and also at Ismani On-station trials in Iringa. These were also<br />

accompanied by their extension officers from the respective villages and from the district. The objective<br />

of the farmer exchange visit was farmers from Mangalali to get experiences from other farmers on<br />

principals of cowpea production, processing and utilization as well as how to manage farmer group and<br />

then compare with the way they do in their own group. Also jointly went to research station to learn new<br />

materials and management at the research station.<br />

15<br />

2009<br />

Fig.3. Farmers discuss with their host<br />

about seed production at research plot<br />

of Mangalali <strong>Research</strong> Group, 2009<br />

At Ismani research station, farmers from both Mkungugu and Mangalali participated at evaluation of the<br />

14 cowpea screening materials planted. The outcome from their selection of the lines according to their<br />

criterion is shown in the Table-15Below.<br />

Table 15: Ranking of the cowpea lines planted at Ismani <strong>Research</strong> station 2009<br />

No Cowpea line Number of Number of Ranking of Comment<br />

participants farmers selected the cowpea<br />

line<br />

1 TZA-263 26 24 1 Each farmer was<br />

2 IT99K-7-21-2-<br />

20 2<br />

given a chance<br />

2-1<br />

of selecting only<br />

3 B 301 9 3<br />

best three<br />

4 ITOOK-1207 8 4<br />

cowpea lines<br />

5 IT96D-733 8 4<br />

6 IT89KD-288 6 5<br />

7 IT99K-573-1 3 6<br />

Table 16: Ranking of cowpea by Ari Mpya Farmer <strong>Research</strong> Group Mkungugu 2009<br />

No Cowpea line Number of Number of Ranking of Comment<br />

participants farmers who the cowpea<br />

selected line<br />

1 TZA-263 23 21 1 Each farmer was<br />

2 IT99K-7-21-2-<br />

17 2 given a chance<br />

2-1<br />

of selecting only<br />

3 IT89KD-288 17 2 best three<br />

4 B 301 9 3 cowpea lines<br />

5 IT99K-573-1 5 4<br />

At both sites farmers chose two best cowpea lines, which are TZA 263 for large grain size and IT99K-7-<br />

21-2-2-1 for its earliness.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Activity 2.1.2b Farmers participate at National Agricultural Show in Dodoma 2009:<br />

Two farmers from each of the farmer groups Msungua (Mshikamano FRG) and Ikhanoda (Mfwenji<br />

FRG) organized by the District Council represented their farmer groups at National Agricultural show,<br />

which took place in Dodoma from 1-8 August 2009. The farmers displayed their cowpea varieties and<br />

products processed from cowpea such as bans. This was a good motivation for the farmers.<br />

Output 2.2: Nutrition status of farmers improved<br />

Activity 2.2.1 Inventory of recipes of cowpea products<br />

This activity was undertaken in 2007/08 and completed and made a basis for the following activities<br />

Activity 2.2.2a: Develop/modify / demonstrate cowpea products:<br />

Introduction: Cowpea (Vigina unguiculata) is an important food legume and an integral part of<br />

traditional cropping systems in the semi arid regions of the tropics (Ryan et al, 1984). Although<br />

indigenous to Southern Africa, cowpeas has spread world wide and is extensively cultivated in semiarid<br />

and consumed in many regions of Africa including Tanzania.<br />

Farmers in the project sites produce many varieties of legumes particularly cowpeas, which are<br />

important for food and cash income for household. Thus make leguminous crops rank second after<br />

cereals such as maize and sorghum. Cowpea is a preferred staple food and its desirability reflects the<br />

fact that the leaves, immature pods, fresh seeds, green pods and dry grains can be eaten or marketed.<br />

The dry grains are commonly eaten whole or can be milled and consumed in numerous traditional main<br />

snack foods (E. Sarakikya, 1996). Cowpea grains as well as the vegetable parts, make major nutritional<br />

contributions to diets. Nutritionally, cowpea plays a critical subsistence role in the diets of many<br />

households by providing nutrients that are deficient in cereals.<br />

In order to add value, cowpea grains need to be processed. The processing of cowpea grains improves<br />

palatability, digestability, acceptability, reduced cooking time and increases market value of products<br />

made from dhal. This section therefore, sought to improve cowpeas utilization through nutrients<br />

addition to different products.<br />

The main objective of this study was to promote the utilization of cowpea in Tanzania.<br />

• Train farmers on the processing of cowpea grains into dhal<br />

• Preparation of cowpea dishes (snack bites from cowpea flour “bagia’)<br />

• Perform sensory evaluation tests.<br />

Expected outputs<br />

• Smallholder farmers’ capacity and capability on ways of using processed cowpea products built.<br />

• Smallholder farmers, skills and knowledge in value addition on cowpea products enhanced and<br />

strengthened<br />

• Cowpea products utilization promoted<br />

• Nutrition status for the families in project sites improved<br />

Methedology<br />

Study area<br />

The training was conducted in Msungua, Ikhanoda, and Nduu in Singida region, Kikombo and<br />

Mpunguzi in Dodoma region and Mkungugu, Ilambilole and Mangalali in Iringa region. The sites were<br />

purposively selected as a result of their involvement in a project on “Improvement of cowpea<br />

productivity on Alectra Vogelii infested land in Tanzania.<br />

Materials used<br />

Materials used to perform and conduct this study include, flip charts, board, cowpea grains, cowpea<br />

flour, cowpea dhal, ingredients such as onions, garlic, eggs, chilies, cooking oil and salt.<br />

16<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Approach<br />

A participatory research approach of working with organized village farmer groups was adopted. These<br />

included participatory research models such as demonstrations, displays and training through hands-on<br />

experience. Appreciative problem solving methods were used to encourage farmers learn, explore and<br />

practice new skills and knowledge.<br />

Sample size<br />

The number of farmers in each group varied between 15-35 members. Level of women representation<br />

was 58 percent of the total number. The study participants were mainly smallholder farmers drawn from<br />

eight villages in three regions. These farmers provided the information on producers and consumers.<br />

These farmers mostly grew cowpeas for subsistence and they were the one who tackled the issue of<br />

consumer preference. Other participants included government officials, district and village extension<br />

officers.<br />

Training of farmers and local processors<br />

A one day workshop was conducted in each project area and farmers were trained on improved cowpea<br />

technologies. The trainings were based on demonstrations, hands-on trials and printed materials<br />

(bronchures for cowpea recipes). Farmers participated in the validation of five recipes for cowpea value<br />

addition. Sensory tests were carried out, where by farmers were required to rank and find out the most<br />

preferred type of”bagia”. The five types of bagia were made using different recipes (see Table 1). The<br />

recipes were translated into national language for better understanding.<br />

Table 18: Cowpea snack product recipes<br />

Bagia type Materials/ingredients<br />

1. Bagia asilia Cowpea dhal/flour, salt, cooking oil, water<br />

2. Bagia asilia na viungo Cowpea flour, salt, cooking oil, garlic, onions and water<br />

3. Bagia; viungo na hamira Cowpea flour, salt, cooking oil, onions, garlic, yeast and<br />

water<br />

4. Bagia; viungo na mayai Cowpea flour, salt, cooking oil, garlic, onions, eggs and<br />

water<br />

5. bagia; viungo, mayai na hamira Cowpea flour, salt, cooking oil, onions, garlic, eggs,<br />

yeast and water<br />

Common method used<br />

• Mix all the ingredients together<br />

• Add water and mix well<br />

• Make the dough by needing (allow the dough to rise by leaving it for 10-15 minutes)<br />

• Heat cooking oil until really hot.<br />

• Use spoon or hand to make the size of bagia you need and put the dough into the oil<br />

• Stir and cook until brown<br />

• Remove from oil and drain<br />

• Cool and pack for marketing or save for consumption.<br />

Farmers were equipped with skills and knowledge on quality control with emphasis on the storage<br />

techniques. Also they were informed about product grading/sorting, market requirements of quality,<br />

quantity and sustainability. The training also emphasized on the methods of de-hulling whole cowpea<br />

grain for dhal production that can be converted into other value added and snack products, such as<br />

“bagia”. The value added products are considered nutritious and sources of income generation. It was<br />

however, observed that the prevailing low production levels, lack of adequate and appropriate storage<br />

facilities constrained the sustainability of the niche markets.<br />

17<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Dissemination of information<br />

A Leaflet on “Recipes for cowpea products” was provided to farmers in each project site. These were<br />

produced in Swahili for better understanding of farmers. It emphasized on the different ways of cowpea<br />

utilization, recipes and methods of de-hulling whole cowpea grain to produce dhal that can be converted<br />

into other value-added products such as dhal, stew and snack products like “bagia”. Value-added<br />

products are considered nutritious and sources of income generation. It was also observed that storage of<br />

cowpea grain was a big problem but dehulling the grains could improve storage up to many years. Also<br />

this reduces the labour involved in preparation by women, wherever they come to prepare Bagia.<br />

It is envisaged the disseminated materials can always be referred to by farmers/ processors in case of<br />

problem shooting long after the project expires.<br />

Results and Discussions<br />

Cowpea preference<br />

The study found that, the current type of cowpea grown in the project sites take more than two hours to<br />

cook. The smallholder farmers/consumers prefer cowpeas that cook in less than two hours. Hence<br />

training of cowpea grain processing into dhal as on alternative of reducing cooking time was important.<br />

Sensory evaluation<br />

“Bagia” samples were prepared using the above recipes. Each bagia type reflected to the type of recipe.<br />

At the time of testing the samples were coded 1,2,3,4, and 5 for bagia types made from recipes 1 to 5<br />

respectively. A panel of respective farmers, extension officers and village government leaders<br />

participated in the trial. They were given the five samples of bagia not simultaneously, one at a time and<br />

they were asked to assign preference score on products attributes including taste, texture and<br />

palatability. A pair-wise ranking method (Table19) was used to find out the most preferred bagia type.<br />

Panelists were asked to taste and rank the overall acceptability of the product in relation to that of the<br />

other four. Every sample was compared to each other by allowing every panelist to give opinion or rise<br />

up hands for most preferred sample. The scores of the quality attributes were done using the following<br />

rating scale;<br />

1 = excellent<br />

2 = very good<br />

3 = good<br />

4 = average<br />

5 = poor<br />

The overall acceptability was ranked from 1-5 (Table 19)<br />

Table 19; Ranked cowpea “bagia”<br />

Bagia type Rank<br />

1 5<br />

2 3<br />

3 4<br />

4 2<br />

5 1<br />

Table 2 shows that, bagia type 5 was the most preferred by farmers, while bagia type 1 was the least.<br />

This indicates that, bagia enriched with nutrients (type 5) came out as the most preferred as compared to<br />

“bagia asilia”(type 1), which was not enriched with nutrients (See Table 19). It is evident that,<br />

palatability and taste to a large extent is associated with nutrients enrichment. According to the panelist<br />

views, it is believed that if a product is palatable and nutritionally enriched, it is most likely to fetch<br />

more market than the reverse. Therefore value added products, apart from being nutritious they are<br />

considered palatable and sources of income generation.<br />

18<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 20: Summary village score and rank data<br />

Bagia type Village scores and rank<br />

Singida region Dodoma region Iringa region<br />

Msungua Ikhanoda Nduu Kikombo Mpunguzi Mkungugu Ilambilole Mangalali<br />

S R S R S R S R S R S R S R S R S R<br />

1.Bagia asilia 0 4 0 5 0 5 4 1 0 3 0 5 0 5 0 5 0.5 3.5<br />

2. Bagi +<br />

viungo<br />

3.Bagia +<br />

viungo+hamira<br />

4. Bagia +<br />

viungo+<br />

mayaia<br />

19<br />

2009<br />

Aver<br />

age<br />

Av.<br />

rank<br />

2 2 2 3 4 1 3 2 3 1 2 3 2 3 4 2 2.8 2.1<br />

1 3 1 4 1 4 0 5 1 2 1 4 1 4 1 4 0.9 3.8<br />

4 1 4 1 2 3 1 4 3 1 3 2 3 2 2 3 2.8 2.1<br />

5.Bagia +<br />

viungo+ mayai<br />

+ hamira<br />

4 1 3 2 3 2 2 3 3 1 4 1 5 1 3 1 3.4 1.5<br />

NB: S= Score, R= Rank<br />

Storage and storage insect pests:<br />

Cowpea grain is very susceptible to storage pests especially to bruchids hence store poorly. This has<br />

been one of the constraints that farmers do not keep much of their cowpea grains more than 6 months.<br />

On the other hand dehuled cowpeas are not infested by storage pests and can bee stored for a long time<br />

so long they are kept in a dry place.<br />

CONCLUSIONS AND RECOMMENDATIONS<br />

Initially, cowpea production has mainly been for household use and limited sales to local/village<br />

markets. Thus by providing farmers with the technologies for use in the improvement of the processing<br />

of cowpea into dhal, this study has opened the community to new avenues for storing the cowpea<br />

products longer and obtaining income from the crop.<br />

By achieving the diversification of small-scale rural farmers’ incomes through adoption of appropriate<br />

post-harvest technologies and providing information on the value addition for cowpeas, the project has<br />

equipped farmers with means of enhancing their household incomes. This will be enhanced through<br />

better quality products which will ensure small-holder farmers’ ability to have market access for them.<br />

Additionally, through equipping farmers with knowledge and skills of improved handling, processing<br />

and utilization of cowpea products, the capacity of the rural communities in which the project has<br />

operated, to earn an income from similar processed products through value addition has been enhanced.<br />

Also the project has built the capacity of the rural small-scale cowpea farmers in working in groups and<br />

organizing themselves to produce necessary volumes and quality requirements cowpea grain which can<br />

meet market requirements. The project further enabled the farmers to change their perception of cowpea<br />

as a crop for household consumption and small scale production, to perceiving it as a possible income<br />

earner with potential to increase household incomes.<br />

However, this study highlighted the fact that, what is needed at times is change in attitude of farmers<br />

towards a crop in order to enable them increase its production and make an income from marketing its<br />

products.<br />

It is recommended that, farmer groups need to be facilitated and equipped with means to obtain storage<br />

facilities for large volumes of cowpea grains, especially since large quantities of grains are expected to<br />

be produced.<br />

Finally, it is recommended that, this study is replicated in other areas of the country and other crops<br />

characterized by low utilization base.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Way forward<br />

There a need to have a follow-up study to evaluate post-harvest technologies taught/taken to smallholder<br />

farmers in project sites. This will be a kind of monitoring and evaluation study, it may be done routinely<br />

to evaluate the rate of adoption of post-harvest technologies taught/taken to farmers.<br />

The study will include the following activities;<br />

• Post-harvest technologies taught<br />

• Cowpea handling, processing, storage utilization and marketing<br />

Indicators<br />

- Level and constraints limiting the use of technologies on cowpea handling, processing, storage,<br />

utilization and marketing<br />

- Processing methods<br />

- Storage techniques<br />

- Forms and rate of cowpea consumption at household and community levels<br />

- Cowpea preferences on for example product taste, texture, grain size, skin colour, type of<br />

products, etc<br />

- Cowpea marketing value chains<br />

- Types of value added cowpea products used and sold at the markets, at what type and level of<br />

markets, Access and market linkages established/existing<br />

- The general level, use and application of improved technologies.<br />

References<br />

Eva Pendeli Sarakikya, 1996, Tanzania Cook Book, Tanzania Publishing Limited,<br />

Dar es Salaam.<br />

J.K. Ryan, P.D. Bidinger, N. Prahlad Rao, p.Pushpamma, 1884, The Determinants of<br />

Indivicual Diets and Nutritional Status in six villages of South India, ICRISAT, India<br />

Activity2.2.2b: Analysis of nutrient content in cowpea grain and products<br />

Studies on micronutrients: Minerals of nutritional importance that were analyzed included iron, zinc<br />

and calcium; results indicate that, there was twice much calcium in leaves than in cowpea grains and<br />

almost 40 times as much iron. Since iron and zinc levels in cowpea leaves have shown to be<br />

significantly higher than the levels in cowpea grains, creating more awareness and encouraging the<br />

utilization of cowpea leaves will contribute in alleviating micronutrient deficiencies from cheap sources<br />

especially from resource-poor families. The variety that was best with regards to all minerals was<br />

IT99K‐7‐21‐2‐2‐1. The cowpea lines that were best with regards to crude protein and fats content<br />

were IT97K499‐8 and IT99K‐7‐21‐2‐2‐1.<br />

Output 2.3: Option for community-based cowpea seed production and increased farmer access to<br />

seed identified:<br />

Activity 2.3.1: Identification of seed multplication mechanism<br />

- Available sources of seed or classes of seed are<br />

- Breeder seed<br />

- <strong>Foundation</strong> seed<br />

- Certified seed<br />

- Quality Declared Seed (QDS)<br />

From these classes farmer groups or communities can produce QDS from foundation seed or certified<br />

seed. The produced QDS can only be sold within the district. However, only trained Farmer<br />

Groups/Farmers can be contracted by Agricultural Seed Agency (ASA) or any other seed company to<br />

produce certified seed that can be sold beyond the production area.<br />

20<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Sub-Activity2.3.1.1: Train farmers to produce seed (QDS) for commercial use:<br />

Training of farmers on Seed Production (QDS) of Cowpea is very important because the crop saves<br />

many lives of people in drought prone areas of the semiarid areas where the crop is grown. The seed<br />

availability is a big issue because commercial companies are not interested in producing seed of low<br />

volume crops like sorghum, pearl millet bambaranuts and cowpea. For this reason it was embedded in<br />

the project to train farmers to produce their own seed which they can use themselves and sell to the<br />

neighbours and neighbouring villages. It is intended also that once these farmers have known the<br />

techniques of producing high quality seeds, they will later be contracted by the Agricultural Seed<br />

Agency (ASA) to produce certied seed to reach many farmers, and not within their district, but to more<br />

districts that grow the crop.<br />

Farmers were given training on the following main seed production areas:<br />

1. Organization of seed production in Tanzania (L 1): This lecture was given in order to expose farmers<br />

on the various institutions/stakeholders involved in the seed industry, where they are and what they are<br />

doing. It also intended to make them understand the seed classes produced in the country.<br />

Lecture components included the following: The roles of Agriculture <strong>Research</strong> Institutions (ARI’s),<br />

Agricultural Seed Agency (ASA), Seed companies, Farmer groups, Seed Certification Institute and<br />

Agriculture Extension Services Department, Seed production flow from ARI’s to Seed companies and<br />

to farmer groups and Seed classes recognized in Tanzania seed industry<br />

2. Attributes of seed quality (L 2): To make farmers understand the concept of seed quality and its<br />

implications in seed production, processing, storage and distribution. The lecture components included:<br />

Importance of seed as a basic input in agriculture production, Merits of improved varieties as a basic<br />

requirement for any variety before releasing to farmers and Seed quality aspects which are verifiable by<br />

already set standards by Seed Certification Institute such as genetic and physical purity, germination and<br />

seed health.<br />

3. Cowpea Seed Production (L 3): Being the core subject, farmers were trained on the techniques used<br />

in the production of cowpea seeds. More emphasis was put in the husbandry practices, which will<br />

enable them harvest high quality seed.<br />

Training components included: Land selection for cowpea seed production, Land preparation, Choice of<br />

varieties to be planted according to days to maturity, Plant spacing depending on whether a variety is<br />

determinate or indeterminate, <strong>Crop</strong> husbandry practices in general such as pest management, rouging of<br />

off types in seed production, timely harvesting, seed conditioning, safe seed storage and seed sampling<br />

and testing for quality attributes of the project should be initiated by 2010.<br />

Table 17: Summary topics covered by each Farmer <strong>Research</strong> Groups, 2009<br />

Region District Village F R G NO. of<br />

participants<br />

SINGIDA Singida<br />

district<br />

DODOMA Dodoma<br />

urban<br />

IRINGA Iringa<br />

district<br />

Topics Covered<br />

Topic Topic Topic<br />

1 2 3<br />

Msungua Mshikamano 10 X X X<br />

Ikhanoda Mfwenji 20 X X X<br />

Nduu Matumaini 21 X X X<br />

Kikombo Ushirika 11 X X<br />

Mpunguzi Twende na<br />

wakati<br />

10 X X<br />

Mkungugu Ari mpya 10 X X<br />

Mangalali Fahari 14 X X<br />

21<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Output 2.4: Cowpea market opportunities identified<br />

Activity 2.4.1: Market survey<br />

The survey was completed 2008 and out of it a paper was presented at the 8 th Scientific Conference of<br />

the Agricultural Economics Society of Tanzania (AGREST) held at Visitors Inn Hotel, Zanzibar, 6 th -8 th<br />

October 2008’<br />

Activity 2.4.2: Marketing chain stakeholders meeting<br />

The meeting was supposed to take place this year but due to poor cowpea harvest, farmers had nothing<br />

to offer to traders. This now has been rescheduled for next season hopping there will be good harvest for<br />

the crop as farmers have enough seed to plant during the season.<br />

22<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 2: Cowpea Utilization in Iringa and Dodoma Regions<br />

Dr. Peter S. Mamiro; Sokoine University of Agriculture, Morogoro<br />

Dr Ambonesigwe. M. Mbwaga, ARI Uyole Mbeya<br />

Introduction<br />

Mature dry cowpeas are important in the diets of many population groups around the world. Africa<br />

alone accounts for 7.5 million hectares of the estimated world total area, of about 10 million hectares.<br />

Of the 7.5 million hectares, about 70% lies in West and Central Africa (Singh et al., 1996). This food<br />

legume is readily available, inexpensive and popular part of the traditional food system. Cowpeas<br />

(Vigna unguiculata L.Walp) along with other legumes are recognized as an important source of protein<br />

(Agazounon et al, 2004). However, their contribution to the overall diet and dietary mineral needs is less<br />

well known. For instance nutritional deficiencies of iron and zinc are often widespread in developing<br />

countries, where staple diets are frequently plant-based and consumption of expensive meat products is<br />

low. Cowpea is an annual legume, cultivated dried fresh grain and long stored dried grain being cooked<br />

while others are dehulled to remove the seed testa, then ground to obtain flour. The flour is used to<br />

make various dishes or recipes. The young and tender cowpea leaves are picked and eaten as relish<br />

along with the main staples. The grains and leaves are the source of carbohydrates, proteins, fats,<br />

vitamin A (ß-carotene), B and C, which are necessary for maintaining good health and fighting disease.<br />

Young cowpea leaves are consumed in at least 18 countries in Africa, and seven countries in Asia and<br />

the Pacific (Ahenkora et al., 1998). Cowpeas are among the top three or four leaf vegetables marketed<br />

and consumed (Barret, 1990). Cowpea also contains iron, calcium, phosphorus, magnesium, copper,<br />

zinc and is high in dietary fibre (Sinha and Kawatra, 2005). The other vegetative parts of the cowpea<br />

plant after removal of the grain, are used as feed, forage, hay and silage for livestock. This paper<br />

reports on the extent of utilization of cowpeas and its contribution to the macro and micro nutrients to<br />

communities that cultivate and consume the legume.<br />

Methodology<br />

Survey<br />

A survey to determine the pattern of utilization of cowpea was conducted in Iringa and Dodoma region<br />

in Kalenga and Msinga villages respectively. The choice of these villages was undertaken in<br />

collaboration with government extension authorities from the two regions. The communities are well<br />

known for the production, selling and consumption of cowpeas. The two regions fall within two<br />

different agro ecological zones,. Iringa being a wet region located in the southern highlands zone and<br />

Dodoma being a semi arid region in the central zone. A simple structured questionnaire seeking<br />

information on the quantity and frequency of consumption of cowpeas and other foods at the homestead<br />

was administered to 511 randomly selected farmers. Samples of local cowpea varieties and products<br />

prepared from cowpeas were collected for analysis in a laboratory at Sokoine University of Agriculture.<br />

The processing and preparation steps of cowpea recipes and products were documented.<br />

Proximate analysis of the samples<br />

The food samples for chemical analysis were grounded to pass through a one millimeter screen in a<br />

Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (% DM) was determined by<br />

drying the sample in an oven at 103 ºC – 105 ºC for 24 hours. Crude protein percentage (% CP) was<br />

determined by Kjeldahl method AOAC method No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030<br />

analyzer, whereby percentage Nitrogen obtained was used to calculate the % CP using the relationship:<br />

% CP = % N x 6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />

System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and percentage ash (%<br />

minerals) was determined immediately after the dry matter determination by incinerating the samples in<br />

a muffle furnace at 550 ºC for four (4) hours. The ash was cooled in a desiccators and then weighed.<br />

Crude fiber percentage (% CF) was determined by using the fiber system and Weende method (AOAC<br />

23<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

1995). Nitrogen free extract percentage (% NFE) was calculated by differences as shown: % NFE = 100<br />

- (% moisture + % CP + % EE + % CF + % Ash).<br />

Total minerals<br />

Total mineral content of the samples was carried out after dry ash. The ash was dissolved in a solution<br />

of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture was 6NHCL. Total iron, zinc<br />

and calcium were determined by atomic absorption spectrophotometry by AOAC method 970.12 No.<br />

(AOAC, 1995).<br />

Data analysis<br />

Data for minerals and proximate analysis were entered in SPSS 12.0 for windows computer software.<br />

Descriptive statistics was used to determine the measures of central tendency for each analyzed cowpea<br />

variety. An analysis of variance of the results was done at 95% confidence interval (P≤0.05) using<br />

Tukeys Honestly Significant Difference. Homogeneity test was performed to determine homogenous<br />

sets.<br />

Results<br />

Demographic characteristics of farmers<br />

There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116 (48%) were<br />

females. In Dodoma 277 farmers were interviewed, 135 (49%) were males and 142 (51%) were females<br />

(Table 1). Farmer’s age ranged between 18 to 83 years with a mean of 45±15 years while in Dodoma<br />

the range was between 20 and 59 years with a mean of 35±7 years. In the two regions, the majority of<br />

the farmers had only studied up to class 7 (57% in Iringa and 88% in Dodoma) which is primary school<br />

level. However, 5% and 1% of interviewed farmers had no formal education in Iringa and<br />

Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up to six family<br />

members.<br />

Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />

Parameter Category Iringa Dodoma<br />

n % N %<br />

Gender Male 124 51.7 135 48.7<br />

Female 116 48.3 142 51.3<br />

Education Class: 3-7 164 70.4 257 92.8<br />

Class 8-14 58 24.2 16 5.9<br />

No education 13 5.4 4 1.4<br />

Household size 1-6 members 192 80.8 183 66.8<br />

7-14 members 48 19.2 94 33.2<br />

Cowpea harvested, procured and utilization<br />

Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own cowpea, or obtained<br />

cowpea supplies from friends and relatives. Few of the farmers purchased cowpea for home<br />

consumption (Table 2). Farmers harvested an average of 75 kg of cowpeas per household with a range<br />

from 53 to 118 kg and the amount sold per household ranged from 4 to 73kg with an average of 25kg<br />

per household. The amount kept at home for consumption ranged from 36 to 97 kg with an average of<br />

52 kg. A substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />

between 100 and 1000 grams of cowpea leaves for household consumption depending on the household<br />

size. Per capital consumption ranged from 40 to 200 gm of cowpea grain for 84% of households in<br />

Iringa and 94% in Dodoma. At the same time per capital consumption for cowpea leaves ranged from<br />

10 to 500 grams by 95% of households in Iringa and 100% in Dodoma, respectively.<br />

24<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

On days when cowpeas are cooked in a household, the same relish will be eaten with either rice or stiff<br />

porridge during lunch, dinner and with breakfast the following morning. More than 50% of households<br />

in Iringa and Dodoma consumed cowpea at least once and up to three times a week (Table 2). Cowpea<br />

leaves were also prepared and eaten on separate days. The leaves are consumed fresh especially during<br />

wet season and in dried form during the lean or dry season. Almost all farmers in the two regions<br />

consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff porridge (ugali), a<br />

mixture of dehulled maize and cowpeas (kande) and most frequently the cowpea buns (bagia).<br />

Table 2: Farmers Responses on Cowpeas Utilization<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Ever eaten cowpea Yes 240 100 277 100<br />

How often eaten per day Twice 92 38.5 156 56.3<br />

Thrice 147 61.5 121 47.7<br />

How often eaten per week Once 77 32.1 28 10.1<br />

Twice 75 31.3 119 43.0<br />

Thrice 65 27.1 82 29.6<br />

Four Times 11 4.6 38 13.7<br />

Five Times 12 5 10 3.6<br />

Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />

Purchase<br />

market<br />

from the 41 17.1 6 2.2<br />

Get from the neighbor 75 31.3 140 50.5<br />

How much cowpea is prepared in one 100-500gms<br />

meal<br />

137 57.1 115 76.9<br />

500-2000gms 103 42.9 64 33.1<br />

How much cowpea leaves are prepared<br />

in one meal<br />

100-1000gms 146 61.9 227 89.7<br />

1000-4000gms 90 39.1 26 11.3<br />

Consumed green cowpea leaves Yes 240 100 277 100<br />

Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />

Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />

20 – 90 minutes 133 65.7 214 79.4<br />

Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />

20 – 90 minutes 31 54.4 72 62.1<br />

How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />

Wash-chop-cook 214 90.7 44 44<br />

Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />

Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />

Cowpea roasted 17 2.1 44 4.8<br />

Roasted milled to flour to prepare<br />

porridge<br />

Cowpea Porridge 28 3.5 66 7.2<br />

Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />

25<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Parameter Category Iringa Dodoma<br />

Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />

Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />

201-500 39 16.2 19 7.0<br />

Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />

500 - 1400 12 5.1 0 0<br />

Mineral Content<br />

Minerals of nutritional importance that were analyzed included; iron, zinc and calcium. Analysis<br />

showed that all varieties had significantly different (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />

Moisture<br />

(%)<br />

27<br />

Ash<br />

(%)<br />

Ca<br />

(mg/kg)<br />

Zn<br />

(mg/kg)<br />

Fe<br />

(mg/kg)<br />

Local Cowpea grain Dodoma 9.40 3.89 958.14 31.45 28.90<br />

Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />

Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />

Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />

Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />

Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />

Local Cowpea grain Iringa 9.56 3.66 992.43 32.56 27.61<br />

Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />

Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />

Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />

Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />

Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />

Proximate composition<br />

Proximate analysis showed that the cowpea varieties with the highest crude protein content were<br />

IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%) Varieties with lowest iron<br />

included IT00K-1207 (22.01%) and TZA 263 (22.6%). For fats, IT97K499-8 (11.18%), IT99K-7212-2-<br />

1 (10.98%) and Fahari (10.33%) varieties had the highest levels while the lowest included IT97K819-<br />

118 (8.17%) and IT96D-733<br />

(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118 (16.1%). The<br />

lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%) and VULI 2 (13.1%). Among<br />

the best new lines with regards to crude protein and fats content were IT97K499-8 and IT99K-7212-2-1.<br />

Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />

Variety/Food component % DM %CP %CF %EE %NFE<br />

VULI 1 89.25 24.02 13.77 9.12 38.18<br />

IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />

IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />

IT96D-733 88.54 23.44 14.91 8.34 37.68<br />

IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />

IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />

FAHARI 89.29 25.46 13.45 10.33 35.86<br />

TUMAINI 89.13 25.96 13.83 9.89 35.23<br />

IT00K-1207 87.95 22.01 15.66 8.46 37.82<br />

IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />

TZA263 89.21 22.60 17.21 8.86 36.45<br />

VULI 2 89.53 25.64 13.10 10.25 36.53<br />

IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />

B301 90.11 23.75 14.85 8.39 38.85<br />

IT99K-721-2-2-1 89.68 26.00 12.53 10.98 35.89<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />

Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />

Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />

Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />

Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />

Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />

Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />

In comparison with the improved lines and varieties, local cowpeas have comparable levels of dry<br />

matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table 5). However, the levels of<br />

fats are relatively lower (5.4%) in comparison with the improved lines/ varieties ranging from 8% to<br />

11.2%. A similar trend is shown with cowpea leaves. Dehulling the cowpeas reduces crude fibre<br />

significantly with a gradual fall in fats. With regard to the cowpea buns from Iringa and Dodoma<br />

regions there was significant drop in crude protein and crude fibre but a very significant rise in fats<br />

(14.3%).<br />

Discussion<br />

Macro and micro nutrient content<br />

In the two surveyed regions it was revealed that cowpeas are produced by majority of the farmers and<br />

consumed to a relatively large extent by all respondents, which provides an indication of cowpea<br />

importance in the two communities. Similarly, the survey revealed that cowpeas are prepared and<br />

consumed in a number of ways, such as relish for stiff porridge and rice, mixed and boiled with dehulled<br />

or un-dehulled maize and cowpea buns. In both regions, the cowpea buns were important as they were<br />

also a source of income in a number of households. The buns however, appear to have lower iron and<br />

calcium, especially those from Iringa region. The calcium, zinc and iron content of the cowpea buns<br />

were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma region had comparatively<br />

higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and iron (16.3mg/kg). This difference might<br />

emanatre from the method of preparation of cowpeas in Iringa in comparison to Dodoma. In Iringa the<br />

grains are dehulled and soaked for about three hours and then washed in flowing river water for about<br />

an hour. This procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />

these minerals are largely lost during washing.<br />

With regard to the deep frying of the cowpea buns, though some nutrients such as proteins and crude<br />

fibre were significantly reduced (from average of 22% to 19% protein), in the process fats were<br />

significantly increased (from average of 5% to 14%), while nitrogen free extracts and dry matter<br />

remained stable. The oil increase is attributed to the sunflower oil used to deep fry the buns. The farmers<br />

also cultivate sunflower for home use and sale. Greater oil intake is better as daily activities require<br />

adequate energy supply.<br />

Analysis of the developed cowpea varieties showed relatively higher protein levels of 24- 26% and 8%-<br />

11% fat content. Similar results have been observed by Horax et al., (2004) where they found cowpea<br />

seed contained 20-25% protein and was rich in essential and non-essential amino acids. Asante et al.<br />

(2006) were investigating on 32 accessions of cowpea seeds, found protein and tannin contents ranging<br />

from 16.4-27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006) proximate<br />

analysis of various infested cowpea samples indicated increases in protein and crude fiber contents and<br />

decreases in moisture, ash, fats/oil and carbohydrates contents when compared with those of the<br />

uninfected samples. Percentage increases in protein and fiber contents ranged between 9.31-30.56%,<br />

and 1.24-15.11%, respectively while percentage decreases were, moisture (3.97-10.23%); ash (3.75-<br />

12.87%); fats/oil (1.43-10.00%); and carbohydrate (1.88-7.50%).<br />

28<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Similarly the developed cowpeas were relatively high in calcium, iron, and zinc. However,<br />

considerable mineral variation was found among the analyzed cowpeas varieties. Similar study by<br />

Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea variety with the highest calcium<br />

concentration of 2096.0 g/g and Zn was detected in only few varieties ranging from 1501.0 g/g to<br />

2071.0 g/g. Analysis of variance of the samples showed significant differences (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed varieties,<br />

which are higher yielding and resistant to Alectra, they will probably be accepted by the communities<br />

and the amount per capita of cowpeas consumed should increase provided they continue to cultivate the<br />

same area of land.<br />

Conclusion<br />

Cowpea grain and leaves are widely consumed in the two surveyed areas. The contribution of micro and<br />

macro nutrients is significant for both developed lines and local varieties but with leaves having greater<br />

mineral content than grain. Therefore awareness-raising within communities to consume more cowpea<br />

leaves is required. However the amount produced per household is low compared to requirements.<br />

Consequently cowpea intake per capita is low, and does not meet the RDA. However, since households<br />

in both areas still have ample land they should be encouraged to increase the area planted as well as<br />

planting higher yielding varieties in conjunction with preferred local varieties. This does however<br />

require further market investigation.<br />

Other important nutrients such as vitamins, amino acid profile and levels of ant-nutritional factors<br />

(tannins and phytates) should also be further investigated. The vitamins and amino acids influence in<br />

nutrition while the antinutritional factors influence on the bioavailability of the nutrients in the body.<br />

Acknowlegement<br />

The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong> <strong>Foundation</strong>, who<br />

facilitated successful survey and laboratory analysis of macro and micronutrients.<br />

References<br />

Agazounon, C., Coulibaly, O. and Houndekon, V. (2004) Analysis of of cowpea processing techniques<br />

into ‘atta’ in Benin. Bulletin de la recherché Agronomique du Benin. No. 46, September 2004.<br />

Ahenkora, K. Adu Dapaah, H. K. and Agyeman, A. (1998) Selected nutritional components and sensory<br />

attributes of cowpea (Vigna unguiculata [L.]Walp) leaves Plant Foods for Human Nutrition 52:<br />

221–229.<br />

AOAC (1995), Official methods of analysis. Association of Official Analytical Chemists methods,<br />

AOAC 16 th edition Nos. 963.13, 968.08, 970.12, 915.03, 920.87. Washington, D.C.<br />

Asante, I. K., Adu-Dapaah, H Addison (2006) Seed weight and protein and tannin contents of 32<br />

cowpea accessions in Ghana. Journal of Tropical Science, 44 (2): 77 – 79.<br />

Barret R. P. (1990) Legume species as leafy vegetables. In: Janick J, Simon J. E. (eds), Advances in<br />

new crops. Portland, OR: Timber Press, pp 391–396.<br />

Horax, R., Hettiarachchy, N. S., and Jalaluddin M. (2004).Preparation, characterization and functional<br />

properties of protein isolate from cowpea (Vigna unguiculata L. Walp.) Dept. of Food Science,<br />

Univ. of Arkansas, 2650 N. Young Avenue, Fayetteville, AR AR 72704, (2) Department of<br />

Agriculture, University of Arkansas, Pine Bluff, AR 71611.<br />

http://ift.confex.com/ift/2004/techprogram/paper_22759.htm.Accessed 19.09.2009.<br />

Mgbemena, I. C., Opara, F, N. and Iwuala, M. O. (2006) The effect of insect pest infestation on the<br />

nutritional composition of cowpea and maize. Department of Biotechnology, Federal University<br />

of Technology PMB 1526 Owerri, Nigeria International Journal of Natural and Applied<br />

Sciences 2 (2): 96-100, 2006.<br />

Mune Mune, M. A., Mbome, L. I. and Minka S. R. (2007) Improving the Nutritional Quality of Cowpea<br />

and Bambara Bean Flours for Use in Infant Feeding, Pakistan Journal of Nutrition 6 (6): 660-<br />

664<br />

Olaleke, A. M., Olorunfemi, O. and Akintayo, T. E. (2006) Compositional evaluation of cowpea (Vigna<br />

unguiculata) and scarlet runner bean (Phaseolus coccineus) varieties grown in Nigeria.<br />

International journal of food, agriculture and environment 4 (2): 39‐43.<br />

30<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Singh B. B., Sharma B. M. and Chambliss O. L. (1996). Recent advances in cowpea breeding. In<br />

Proceedings of the Second World Cowpea <strong>Research</strong> Conference, 5–8 September 1995, Accra,<br />

Ghana. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.<br />

Sinha, R. and Kawatra A. (2005) Effect of Processing on phytic acid and polyphenol contents of<br />

cowpeas (Vigna unguiculata (L) Walp. Plant Foods for human Nutrition, 58 (3): 1‐8.<br />

Wang, N., Hatcher, D.W., Toews, R. and Gawalko, E.J. (2009) Influence of cooking and dehulling on<br />

nutritional composition of several varieties of lentils (Lens culinaris) LWT ‐ Food Science and<br />

Technology, 42, (4): 842‐848.<br />

Weinberger, K., John Msuya, J. and Mamiro, P. (2004) Domestication of selected African indigenous<br />

vegetables in Tanzania – an ex‐ante impact assessment. AVRDC – The World Vegetable Center,<br />

pp22.<br />

31<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Annex 3: Malawi annual report:<br />

Malawi annual report 2008-09 season (third year)<br />

Prepared by V.H. Kabambe, E. Kazila, A. Mangwela, J Bokosi<br />

CC Mtambo, T. Chilongo and E. Mazuma<br />

1. Field evaluation of promising varieties<br />

1.1 Sites and entries.<br />

On farm trials to evaluate performance and adaptation of outstanding lines were conducted in the<br />

2008/09 season. Trial sites were Bunda College in Lilongwe, Mgwangwa EPA in Lilongwe, Mpokwa<br />

EPA in Zomba, Rivirivi EPA in Balaka, Chitedze <strong>Research</strong> Station and Kasungu. Kusungu and Balaka<br />

are new sites which were added in order to consolidate the genotype x environment evaluation, which<br />

would provide strong evidence on adaptation zones for any new entry which we may have to formalize<br />

for release. Balaka is a low altitude site with low and erratic rainfall. Kasungu is a mid-altitude site<br />

with sandy soils. The rainfall was normal and fairly distributed for all sites (Table 1).<br />

Table 1. Mean monthly rainfall (mm) for the trial sites in 2008/09 season.<br />

Month Site<br />

Bunda Ngwangwa Rivirivi Chitedze<br />

October ‘08 18.5 0 3.0 45<br />

November ‘08 119.2 51 113.4 112<br />

December ‘08 177.1 147 242.1 116.4<br />

January ‘09 291.9 198 300.5 227.8<br />

February ‘09 174.0 152 145.1 121<br />

March ‘09 218.3 134 125.1 223.7<br />

April /09 28.0 71 6.3 18.1<br />

May ‘09 0 0 0 0<br />

Monthly total 1026.7 753 932.5 864<br />

Seven of entries selected for evaluation based on Alectra resistance, farmer’s preferences on grain<br />

characteristics and according to evaluations conducted in the last two years. Table 2 gives brief<br />

highlights about the entries based on preliminary yield evaluations. There were about 25 farmers at 4<br />

on-farm sites conducting the trial, each farmer acted as a replicate. At Chitedze and Bunda College the<br />

trials were complete block design, each with 4 replicates. The trial plots had 5 row plots, 4 m long and<br />

0.75 m apart.<br />

Table 2: Brief descriptions of the entries under evaluations in 2008/09 season, Malawi.<br />

Code Name Description<br />

5 IT98K-503-1 Has good yield on overall, medium seed size, very good Alectra<br />

vogelii resistance<br />

37 IT97K-825-15 Good Alectra resistance, fair yield, medium seed size, low farmer<br />

preference<br />

9 IT99K-7-21-2- Best Alectra resistance in ’07, ‘07/08, preferableseed size, , good<br />

2-1<br />

yield<br />

21 IT99K-494-6 Medium yield, good Alectra resistance in ‘06/07, ‘07/08, medium<br />

seed size, low farmer preference<br />

53 Sudan -1 Released variety, good resistance to Alectra and good yielder in<br />

‘07/08<br />

52 IT82E-16 This is a released variety. Yielded second best across sites in 07/08.<br />

32<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Poor resistance to A. vogelii. Well liked by farmers.<br />

54 Farmers’ local Collected from around trial sites, usually bushy and long maturing.<br />

1.2 Screening for Alectra resistance.<br />

The sites at Ngwangwa and Kasungu had high infestation of Alectra, even though yields were good..<br />

However, within the plots, spots that had high numbers of Alectra were causing stunted growth and<br />

wilting on cowpea plants. Observations on Alectra resistance showed that in sites with high pressure, all<br />

entries succumbed to the parasite, though differentilly. In lightly infested sites, some entries showed<br />

resistance (Table 3). .At Mngwangwa, where pressure was high, the same lines showed fair resistance.<br />

At Lisasadzi, with high Alectra pressure, entries IT97K-825-15 and IT99K-494-6 showed good<br />

resistance. At Bunda College, with high Alectra pressure, the same three lines showed resistance. On the<br />

overall, entry 21 (IT99K-494-6) and entry 9 (IT99K-7-21-2-2-1) were best for resistance with 0.28 and<br />

0.29Alectra plants m -2 respectively, and entry 21 having zero Alectra count at Bunda. Depending on<br />

yield and other results, one of these three could be released. However, all these have longer maturity<br />

periods.<br />

As these observations were done in presence of local extension and farmers, this gave a good<br />

opportunity to orient farmers to the Alectra problem and the objectives of the project. Since the entries<br />

in the trial were combination of early and late maturing types, the general farmers preference at all sites<br />

was for the early types IT82E-16 and Sudan 1. At Kasungu farmers particularly noted that IT97K-825-<br />

15 was undesired due to its late maturity, as it would need more rains. Some of the other reasons for<br />

preferences were grain size (large size preferred).<br />

Table 3 Alectra counts m -2 taken after flowering at all the trial sites<br />

Entrycode<br />

name<br />

& Bunda Lisasadzi Ngwangwa Mpokwa Rivirivi Chitedze Mean<br />

5=IT98K-503-1 1.35 0.50 3.33 0.6 0.0 0.05 0.97<br />

37=IT97K-825-<br />

15<br />

0.08 0.70 0.60 0.03 0.08 0.25 0.29<br />

9=IT99K-7-21-2-<br />

2-1<br />

0.25 1.38 1.46 0.11 0.0 0.17 0.56<br />

21=IT99K-494-6 0.00 0.60 1.02 0.04 0.03 0.0 0.28<br />

53=Sudan-1 5.82 3.56 4.03 0.86 0.0 0.58 2.48<br />

52=IT82E-16 6.25 6.70 2.86 0.96 0.14 0.0 2.82<br />

54=Farmers’<br />

local<br />

2.80 1.14 3.29 - 0.03 0.11 1.23<br />

Mean 2.36 2.08 2.43 0.44 0.04 0.17 1.25<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

traits in entry 9 and 21 can be reflected in the progenies. The project is so far promoting the released<br />

varieties into its outreach progammes.<br />

Results on number of plants/m 2 are shown in Table 5and they show that there were significant<br />

variations in plant establishment. Looking at yield data, entries 21, 52 and 53 which gave highest yields<br />

also had the best plant establishment.<br />

Table 4:. Grain yield kg/ha of cowpea entries at 6 trial sites in 2008/09.<br />

Entry Site Mean<br />

Lisasadzi Mpokwa Bunda Balaka Mgwangwa Chitedze<br />

5=IT98K-503-1 2034 461 1496 749 856 1667 1210<br />

9=IT97K-7-21-2-<br />

2-1<br />

1749 353 1665 903 543 1222 1073<br />

21=IT99K-494-6 2345 562 1846 1372 1114 972 1369<br />

37=IT99K-825- 1527 447 1276 589 796 1083 953<br />

15<br />

52=IT82E-16 1936 896 1631 2156 903 3194 1786<br />

53=Sudan-1 2328 1402 1728 2158 1070 2278 1827<br />

54=Local - 353 794 964 536 1861 902<br />

Mean 1936 681 1492 1270 831 1754 1327<br />

LSD 523 544 830 878 435 1340<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

9=IT97K-7-21-2-<br />

2-1<br />

10.4 9.0 13.0 10.5 10.0 8.6<br />

21=IT99K-494-6 10.2 11.0 10.8 10.7 11.1 10.8<br />

37=IT99K-825-<br />

15<br />

8.8 10.5 10.7 10.2 10.6 10.2<br />

52=IT82E-16 13.8 13.4 11.5 15.0 14.1 13.5<br />

53=Sudan-1 16.2 17.0 14.8 17.2 16.1 16.3<br />

54=Local - - 16.0 16.0 10.0 14.0<br />

Mean 11.3 11.5 12.3 12.7 12.3 12.0<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

54=Local 3.0 3.5 3.2 4.8 5.0 4.9<br />

Mean 2.5 3.4 3.0 3.7 5.9 4.8<br />

P 0.43 0.60 0.43 0.09<br />

LSD 0.88 1.0 2.40 1.7<br />

CV% 24 21 44 19<br />

Table 9. The effect of genotype on early and late season Cercospora leafspot Incidence (5 plants<br />

affected) and scores (scale 1=clean, 9=severe) of Chitedze <strong>Research</strong> Station.<br />

Entry Cercospora Incidence Cercospora leafspot infection<br />

Early Late Mean Early Late Mean<br />

5=IT98K-503-1 26.6 72.5 49.6 4.2 6.5 5.4<br />

9=IT97K-7-21-2-2-1 21.3 73.8 47.6 5.8 7.3 6.5<br />

21=IT99K-494-6 27.3 68.9 48.1 5.0 7.0 6.0<br />

37=IT99K-825-15 20.8 73.2 47.0 4.8 6.8 5.8<br />

52=IT82E-16 19.2 64.4 41.8 4.8 6.0 5.4<br />

53=Sudan 27.2 75.2 64.4 5.3 7.2 6.3<br />

54=Local 32.7 70.2 75.2 5.8 6.2 6.0<br />

Mean 25.0 71.2 48.1 5.1 6.7 5.9<br />

P 0.38 0.80 0.44 0.14<br />

LSD 13.3 15.2 1.6 1.0<br />

CV% 36 23 22 11<br />

1.5 Days to flowering and maturity<br />

The variations of cowpea entries on days to 50% flower and to maturity are shown in Table 10 and 11.<br />

Table 10 shows that on average plants flowered earliest at Mpokwa (46 days from planting), closely<br />

followed by Mngwangwa (47 days). Mean date to flowering was most delayed at Lisasadzi in Kasungu,<br />

mainly due to the influence of the local variety which took 89 days to flower. In general Sudan-1 was<br />

the earliest to flower across all sites, followed by IT82E-16. The local entry was the last to flower at<br />

Mngwangwa and Lisasadzi only. In general, there were small differences between flowering dates of the<br />

improved varieties (no more than 10 days), but the farmers highlighted that these differences were<br />

significant and important. Regarding days to maturity, the mean maturity period was shortest at<br />

Mpokwa and Balaka, which was expected. These sites are warmer and drier historically. Apart from<br />

the local entry, entries 9 and 37 had the longest maturity periods of about 77 days. This agrees with<br />

observations in the fields and farmers reasons for disliking the entries. Although these entries were late,<br />

they were inferior in yield compared to the early maturing released varieties. This further suggests good<br />

adaptability for the released varieties.<br />

Table 10. Effect of genotype on days to 50% flower at 5 sites, 2008/09<br />

Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />

5=IT98K-503-1 46 52 57 46 48 50<br />

9=IT97K-7-21-2-2-1 48 54 59 53 53 53<br />

21=IT99K-494-6 46 52 57 46 47 50<br />

37=IT99K-825-15 47 56 58 53 52 53<br />

52=IT82E-16 44 48 55 39 46 46<br />

53=Sudan-1 46 47 59 41 51 49<br />

54=Local 50 53 89 - 52 61<br />

Mean 47 52 62 46 50 51<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

CV% 4 4 9 7 26<br />

Table 11 Effect of cowpea line on days to maturity at five sites, 2008/09 season.<br />

Entry Mngwangwa Bunda Lisasadzi Mpokwa Balaka Mean<br />

5=IT98K-503-1 79 82 73 64 61 72<br />

9=IT97K-7-21-2-2-1 80 83 76 73 70 76<br />

21=IT99K-494-6 80 82 71 65 60 72<br />

37=IT99K-825-15 83 88 72 74 69 77<br />

52=IT82E-16 74 75 60 59 66 67<br />

53=Sudan-1 74 74 68 60 69 69<br />

54=Local 80 83 107 - 68 84<br />

Mean 78 81 76 66 66 73<br />

P


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Table 13. Proportion of farmers preferences of trial lines based on selected criteria<br />

Entry Criteria Site or village<br />

Bala Bunda Mngwa Mpokwa Mpokwa Lisaka<br />

ngwa Khuvinda CChibisa Sadzi<br />

vge Vge<br />

n 24 40 17 40 39 32<br />

5=IT98K-503-1 Seed size 4 100 100 90 10 34<br />

Plant type 0 100 100 5 0 59<br />

Early maturity 0 100 100 0 100 3<br />

Insect resistant 0 100 100 0 0 28<br />

Disease resistant 0 100 100 0 25 38<br />

9=IT97K-7-21-2-2-<br />

1<br />

Seed size 100 100 65 60 100 53<br />

Plant type 100 40 41 35 0 63<br />

Early maturity 0 100 47 0 0 0<br />

Insect resistant 71 100 100 0 0 0<br />

Disease resistant 71 100 100 0 0 0<br />

21=IT99K-493-6 Seed size 100 25 71 55 60 50<br />

Plant type 4 0 88 0 26 81<br />

Early maturity 100 0 41 45 67 100<br />

Insect resistant 0 0 100 0 44 100<br />

Disease resistant 0 0 100 0 21 63<br />

37=IT99K-825-15 Seed size 100 70 0 100 38 0<br />

Plant type 0 100 41 15 0 44<br />

Early maturity 0 83 0 0 0 0<br />

Insect resistant 0 100 100 25 0 0<br />

Disease resistant 0 100 100 15 0 13<br />

52=IT82E-16 Seed size 71 35 91 75 28 59<br />

Plant type 100 40 100 100 49 63<br />

Early maturity 100 55 100 100 62 81<br />

Insect resistant 0 60 100 100 40 57<br />

38<br />

2009<br />

Disease resistant 0 50 100 100 26 100<br />

Seed size 75 83 88 75 21 82<br />

53=Sudan Plant type 100 100 100 100 49 68<br />

Early maturity 100 100 100 100 59 100<br />

Insect resistant 100 100 100 100 59 100<br />

Disease resistant 100 15 100 100 77 59<br />

Farmers local Seed size 100 50 0 65 39 19<br />

Plant type 100 0 0 100 41 19<br />

Early maturity 0 75 0 0 0 0<br />

Insect resistant 0 0 0 100 72 0<br />

Disease resistant 0 0 0 100 62 0<br />

1.7 GENERAL DISCUSSION<br />

The results of the field test have been informative due to close association between yield data, yield<br />

components and agronomic traits. The interesting point is that the earlier maturing released varieties<br />

gave highest yields. In general, longer maturity varieties are expected to give higher yields, the reason<br />

for low yields could be low yield potential, or uncontrolled insect pest attack. The key observations on<br />

the entries are summarized in Table 14 below. All entries were equally prone to pests and diseases. The


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

high yields recorded under famer’s conditions are encouraging for them to grow cowpea for food and<br />

income generation.<br />

Table 14. Key traits and strengths of trial entries<br />

Entry Key traits and strength<br />

5=IT98K-503-1 Low yielder, medium maturity (64 days), disease prone, poor farmer<br />

preference, medium Alectra resistance<br />

9=IT97K-825-15 Large seed size, low yielder, good Alectra resistance, medium farmer<br />

preference, long maturity period (73 days), disease and pest prone<br />

21=IT99K-7-21-2-2- Good seed size, medium maturity (65 days), very good Alectra resistance,<br />

1<br />

prone to pests, earmarked for release.<br />

37=IT99K-494-6 Poor farmer preference, late maturity (74 days), very good Alectra<br />

resistance, poor yielder, prone to disease and insect pests<br />

52=Sudan-1 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />

maturity, released variety<br />

53=IT82E-16 Low Alectra resistance, high yielder, small seeded, liked by farmers, early<br />

maturity, released variety<br />

54=Local Variable traits depending on source, largely late maturing, pest prone,<br />

medium yields<br />

2. INCORPORATION OF ALECTRA RESISTANCE INTO ADAPTED VARIETIES<br />

This work is in progress. The released lines are being upgraded to incorporate Alectra vogelii resistance.<br />

The donor lines for Sudan-1 are IT97K-820-18, IT99K-1060, IT99K494-6, IT97K-7-21-2-2-1, and<br />

IT93K-452-1. The donor linefor IT82E-16 is 1T81D-994. The work is at backcross 2 stage to be<br />

followed by selfing.<br />

3. COLLABORATIVE STUDENT WORK<br />

3.1 BSc student (Cowpea variety x time of planting x density trial)<br />

During the season an undergraduate student undertook a study on cowpea time of planting x variety x<br />

density trial. The objective was to determine how these factors interact to optimize crop yields with<br />

respect to rainfall and the need to plant late to avoid disease and insect pressure. Preliminary<br />

observations showed that early planting was the best for yield and less disease infection. This was<br />

because there was a dry spell soon after the first planting. This might have led to conditions not<br />

favourable for the build up of the diseases.<br />

3.2: MSC Student (Ms Elida Kazira - Farmer Participatory Selection of Alectra Resistant Cowpea<br />

Varieties in Selected Districts of Malawi).The student has submitted her thesis which is in the hands of<br />

examiner.<br />

4. CAPACITY BUILDING AND SCALING OUT OF IMPROVED COWPEA LINES<br />

4.1 Primary seed support<br />

The project provided small packs of seed of 0.5 kg each to all project participating farmers Mpokwa and<br />

Mngwangwa EPA (about 60 farmers at each EPA). Participating farmers in Kasungu were also given<br />

this support. Many farmers planted this in pure stand while others planted this in intercrop with maize.<br />

The objective of this program was to provide an experience for the farmers to grow cowpea intensively<br />

(as sole crop) and to practice pest management and observe results. Some of the farmers we monitored<br />

managed to get good yields and were motivated to produce cowpeas. We have helped these farmers to<br />

store some of their seeds so that they can plant more cowpeas next season. During field days and visits<br />

farmers were trained on principles of cowpea seed production. Farmers were also encouraged to use<br />

their cowpea to practice the different recipes which they learned from the project.<br />

4.2 Agriculture fair participation and sharing of cowpea recipes<br />

In June 2009 the farmers from Mngwangwa EPA, on their own accord, organized themselves to<br />

participate at a District Agriculture Fair held in the same EPA. The farmers demonstrated a range of<br />

39<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

cowpea products based on the training made by the project and shared this with participants (Figure 1).<br />

In addition, the project team participated at a national agriculture fair, alongside the <strong>Crop</strong> Science<br />

Department. Again, the focus of the exhibit was of utilization. The exhibits attracted a lot of attention<br />

from participants.<br />

Figure 1. Farmer group displays of cowpea recipes (left) and cross section of participants (right)<br />

at ADD – wide show in Lilongwe.<br />

4.3 Group dynamics training for farmers in Ngwangwa<br />

Group dynamics training was conducted for all the farmers in the two participating villages in<br />

Mgwangwa. The training was conducted in August 2009. The training was provided on group<br />

leadership and operation. This group strengthening included membership and responsibilities,<br />

constitution, networking and fund raising to sustain the groups.<br />

4.4 Workshops on cowpea market value chain<br />

Cowpea market value chain workshops were conducted in Lilongwe and Zomba in August 2009. These<br />

workshops were held following the conclusion of a market survey. As per project document, the,<br />

meetings were held to bring together representatives of cowpea producers, middlemen and traders. The<br />

objective was to link producers better to the market and identifies increased marketing opportunities.<br />

There were lead presentations made by project team theme leaders as follows:- a) a presentation on<br />

cowpea production trends, production requirements, cowpea agronomy, potential yields reasons for low<br />

yields in Malawi and cowpea storage Mr E. Mazuma; b) a presentation on cowpea utilization, including<br />

nutritional value, processing of leaves and grain, utilization as animal feed by Dr Mangwela and c)<br />

presentation on cowpea preferences and marketing by Mr Thabie Chilongo. At both venues there were<br />

representatives of farmers, government extension, NGO’s, agricultural input suppliers, traders and<br />

researchers.<br />

The workshop revealed that there are six big business traders who buy cowpea seeds (ADMARC, Rab<br />

Processors, Mulli Brothers, Agora, Grain Traders Association, and Transglobe). Many of them placed<br />

emphasis on large seeded grain types as preferences. A representative of ADMARC indicated that this<br />

season they have been instructed to buy large seeded cowpea only. The traders acknowledged that they<br />

don’t buy much cowpea, but that the markets were small as well thus can satisfy it. Some cowpea is<br />

sold locally (for cakes and cookie making) while most of it is exported. However, it was noted that most<br />

of the cowpeas are traded in local markets with no clear records on volumes and quality requirements.<br />

At the meetings we displayed a draft version of our booklet on cowpea production and utilization. The<br />

participants demanded speedy release of this book. The nutrition officers collected the draft copies for<br />

immediate use. The workshops also noted that there are many reasons for variety choice amongst<br />

40<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

farmers, such as maturity period, plant stature (whether climbing or creeper), seed size, stay green<br />

characteristics (for prolonged leaf harvesting), seed size and pest resistance. Participants resolved that<br />

some of these preferences were minor and suitable for backyard crop for home consumption. It was thus<br />

resolved that researchers should focus on high yield and pest resistance and commercially desirable<br />

traits such as large seeds. Farmers should be advised to plant small patches of local varieties for<br />

vegetables and green pods.<br />

At the Lilongwe Workshop the research team established links with IITA, which has just won a grant to<br />

work on cowpea. The initial collaboration will cover scaling out issues and market and preferences<br />

surveys. At the Zomba workshop we established a partnership with World Vision Area Development<br />

project officer to facilitate seed supply for groups of farmers for scaling out. They already have<br />

experience on community-based seed production. Similar relationships were established with the<br />

District Agriculture Development Officer for Phalombe.<br />

4.5 Cowpea market survey<br />

This work has been conducted and results are being analyzed, a separate report will be prepared. Very<br />

preliminary results were shared during workshops on cowpea market value chain.<br />

4.6. Policy workshop on seeds<br />

The Malawi country lead participated in a workshop bringing together relevant policy officials to<br />

discuss issues that can facilitate the scaling out of legume seed systems and Integrated Soil Fertility<br />

Management. The workshop was jointly organized by the <strong>McKnight</strong> Legume Best Bets and Seed<br />

Systems projects.<br />

The major policy issue on seed systems, which was a matter of concern, was the seed registration and<br />

certification process. The key concern was t based on the fact that process is too centralized. A seed<br />

grower is required to register at Chitedze <strong>Research</strong> Station, and only the same can certify. The<br />

registration fee is high, particularly for small holder farmers or community group whose cultivated area<br />

is 1-2 ha. The registration fees and inspection costs will erode profits. The argument was that legumes,<br />

self pollinated, have simple requirements for isolation, and rouging for disease and off-types can be<br />

done at District level. Any seed not going through the process cannot be packed and sold on shelves,<br />

then killing any entrepreneurship. The dissemination of legume seeds is directly linked ISFM because<br />

the same do make some N additions to soil. These concerns and other suggestion were raised for<br />

consideration by policy makers.<br />

5: Way forward<br />

Based on the above, the project team would like to make the following recommendations for future<br />

research action.<br />

1. Evaluate the lines again this season and earmark entry 21 for release owing to its good Alectra<br />

tolerance and medium yield<br />

2. Noting the lack of resistance to disease and pests across lines, to intensity the work on crop<br />

protection. This work should include clear definitions of critical minimum sprays required to<br />

keep pesticide costs and labour down. Also to intensity studies on use of botanical pesticides<br />

such as neem, moringa, Tephrosia vogelii and others.<br />

3. To refine the issues of time of planting in line with variety maturity periods in order to avoid<br />

pests and for drought management purposes.<br />

4. To intensify incorporation of resistance into the adapted lines and conduct adaptation trials.<br />

5. To extend primary seed support programme to all testing sites and to willing partners.<br />

6. To request for extension of the project with the objective of completing tasks above. To release<br />

new Alectra resistant at the end of renewal, as well as to establish a seed base to support the<br />

scaling out of the new varieties.<br />

41<br />

2009


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

42<br />

2009<br />

7. Continue with introgression of Alectra resistance in adapted cowpea varieties at Bunda College<br />

if extension of the project has been granted. At the end of the first phase we shall have reached<br />

backcross four.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />

Team Report<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Project leader: Dr AM Mbwaga, ARI Uyole, Box 400 Mbeya, Tanzania. Email: ambwaga@yahoo.co.uk<br />

Team report for 2008 ‐ 2009<br />

Team activities<br />

Team members from Malawi and Tanzania had a number of opportunities to meet during the year.<br />

These included the CoP2 meeting in Maputo, Mozambique in October 2008. This event allowed<br />

representatives from each country to discuss progress and were followed up with a visit by the project<br />

leader Dr Mbwaga accompanied by Dr Mligo, legume breeder, Dr Charlie Riches (CoP- liaison officer) to<br />

Lilongwe in March 2009 and met by the Malawian project team that was composed of Dr Vernon<br />

Kabambe country project leader, Mosses Mamiliro (breeder), and James Bokosi (breeder). The team<br />

visited several project sites, which included Lilongwe district in Ngwangwa Extension Planning Area<br />

(EPA), Kasungu district in Lisasi EPA, Zomba district in Mpokwa EPA and Balaka district in Rividivi<br />

EPA where farmer groups are undertaking on‐farm trials with the project. The wrap up discussions at<br />

Bunda College, University of Malawi came up with the following observations/recommendations; -<br />

released varieties showed better yields than test lines, early maturing varieties save as source of food in<br />

hunger periods, seed system for cowpea is very poor, more cowpea seed should be sent to Balaka and<br />

Kasungu districts as there was high demand for seed of the improved cowpea varieties and lastly farmers<br />

in Rivirivi were recommended to form farmer research groups for better access by researchers and<br />

extension staff as compared to individual isolated farmers<br />

Representatives of project partner institutions travelled together to assess progress with project<br />

activities in Tanzania starting on 27 th April, 2009 in Iringa and ending on 30th April at Ilonga Agricultural<br />

<strong>Research</strong> Institute, Kilosa in Tanzania. Evaluation team members were: Joseph Mligo (Breeder, ARI<br />

Ilonga Kilosa Tanzania); Vernon Kabambe (co‐ordinator for Malawi, Bunda College Malawi); James<br />

Bokosi (Breeder, Bunda College of Malawi), Boukar Ousmane (cowpea breeder IITA Kano Nigeria) and<br />

Ambonesigwe Mbwaga (Project leader, ARI Uyole Mbeya Tanzania). The team was joined by Extension<br />

staff working with the project and other staff members at various sites in each district of Tanzania. From<br />

the evaluation it was noted that drought (rainfall, poor distribution and low amounts) has been an<br />

overriding problem this season. The team commended the collaboration the project is undertaking along<br />

with the tropical legumes II project of IITA. It was recommended to incorporate both traits (Alectra<br />

resistance and Drought tolerance) in the breeding programme. An analysis of medium term climatic data<br />

(20-25 years) would be important to determine the frequency of rainfall events (e.g. start dates, dry spells<br />

and rainfall amounts). This can help to determine the important drought traits in plants (e.g. earliness or<br />

resilience). The good production practices should be incorporated in the screening of the varieties against<br />

Alectra. Hence, there is a need to expose the farmer groups involved in the project to all these production<br />

practices.<br />

The experiences observed from cowpea/Alectra and TL II projects indicate that the overriding preference<br />

trait by farmers is seed size; large seeded are more liked than small seeded types.<br />

The backcrossing programme at ARI Ilonga will continue to backcross 6 by October 2010, while at<br />

Bunda College will be at back cross 4. There will be an urgent need to request for an extension of the<br />

project as the intended cowpea materials will have not reached the farmers at the end of the funding year<br />

2010.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06‐741<br />

Team Report<br />

Insights and lessons learned<br />

During the monitoring tour the team found that the activities planned for 2009 were underway. This was<br />

continuation of activities started in year 1 and 2, including breeding for Alectra resistance and on-farm<br />

evaluation of promising cowpea lines, market surveys, cowpea processing and utilisation as well as new<br />

work on cowpea macro and micro nutrient analysis in cowpea products. Also introduced to the project<br />

was the training of farmers on seed production i.e. production of Quality Declared Seeds (QDS).<br />

There was an outbreak of cutworms on the demonstrations planted in Singida and this gave a learning<br />

lesson that cowpea seed should be dressed with insecticide to prevent damage by cutworms especially at<br />

two leaf stage.<br />

Variability was observed in the reaction of individual cowpea lines to Alectra across sites. Seed was<br />

therefore provided from a range of locations in both countries for a glasshouse screening trial at Long<br />

Ashton UK. This confirmed variability in the host range of parasite samples from Malawi and Tanzania<br />

At a species level there are 3 strains of Alectra by host range: Alectra from Singida in Tanzania attaches<br />

and emerges on cowpea and groundnut but not on mung or common bean. Alectra from Bihawana and<br />

Ismani in Tanzania attaches and emerges on cowpea, groundnut, and common bean but not on<br />

mung bean. Alectra from Malawi sites attaches and emerges on cowpea, groundnut, common bean and<br />

mung bean. Within cowpea, patterns of virulence are evident: Alectra from all sites in both countries<br />

emerges on IT36E-16 and TZA 263 but not on B301. There is need to confirm this by using<br />

biotechnology techniques.<br />

Three selected cowpea lines with traits farmers like, including IT99K-7-21-2-2-1, IT99K-753-1 and TZA<br />

267 are being increased under irrigation at Ilonga <strong>Research</strong> Station to be distributed to many farmers for<br />

wider evaluation in the 2009/2010 season<br />

Due to poor cowpea crop performance during this season, the intended market stakeholder’s meeting<br />

was postponed to next season in Tanzania but it was undertaken in Malawi and a report is being<br />

prepared.<br />

A paper titled “assessment of Cowpea Marketing Efficiency: A case study of selected regions in<br />

Tanzania” by Gabriel, M.; Hella, J.P. and Mbwaga, A.M was presented at the Annual Agricultural<br />

Economists Society of Tanzania (AGREST) held in Zanzibar and will appear in AGREST conference<br />

series Vol. 8. (in press). The proceedings will be ready within and outside the country. Mr Gabriel<br />

Martin’s got financial support from the project for his MSc research work


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Cowpea Utilization in Iringa and Dodoma Regions<br />

Peter S. Mamiro 1 , A. M. Mbwaga 2<br />

Introduction<br />

Mature dry cowpeas are important in the diets of many population groups around the<br />

world. Africa alone accounts for 7.5 million hectares of the estimated world total area, of<br />

about 10 million hectares. Of the 7.5 million hectares, about 70% lies in West and Central<br />

Africa (Singh et al., 1996). This food legume is readily available, inexpensive and<br />

popular part of the traditional food system. Cowpeas (Vigna unguiculata L.Walp) along<br />

with other legumes are recognized as an important source of protein (Agazounon et al,<br />

2004). However, their contribution to the overall diet and dietary mineral needs is less<br />

well known. For instance nutritional deficiencies of iron and zinc are often widespread in<br />

developing countries, where staple diets are frequently plant-based and consumption of<br />

expensive meat products is low. Cowpea is an annual legume, cultivated dried fresh grain<br />

and long stored dried grain being cooked while others are dehulled to remove the seed<br />

testa, then ground to obtain flour. The flour is used to make various dishes or recipes. The<br />

young and tender cowpea leaves are picked and eaten as relish along with the main<br />

staples. The grains and leaves are the source of carbohydrates, proteins, fats, vitamin A<br />

(ß-carotene), B and C, which are necessary for maintaining good health and fighting<br />

disease. Young cowpea leaves are consumed in at least 18 countries in Africa, and seven<br />

countries in Asia and the Pacific (Ahenkora et al., 1998). Cowpeas are among the top<br />

three or four leaf vegetables marketed and consumed (Barret, 1990). Cowpea also<br />

contains iron, calcium, phosphorus, magnesium, copper, zinc and is high in dietary fibre<br />

(Sinha and Kawatra, 2005). The other vegetative parts of the cowpea plant after removal<br />

of the grain, are used as feed, forage, hay and silage for livestock. This paper reports on<br />

the extent of utilization of cowpeas and its contribution to the macro and micro nutrients<br />

to communities that cultivate and consume the legume.<br />

Methodology<br />

Survey<br />

A survey to determine the pattern of utilization of cowpea was conducted in Iringa and<br />

Dodoma region in Kalenga and Msinga villages respectively. The choice of these villages<br />

was undertaken in collaboration with government extension authorities from the two<br />

regions. The communities are well known for the production, selling and consumption of<br />

cowpeas. The two regions fall within two different agro ecological zones,. Iringa being a<br />

wet region located in the southern highlands zone and Dodoma being a semi arid region<br />

in the central zone. A simple structured questionnaire seeking information on the quantity<br />

and frequency of consumption of cowpeas and other foods at the homestead was<br />

1<br />

1.Department of Food Science and Technology, Sokoine University of Agriculture, P. O. Box 3006,<br />

Morogoro, Tanzania.<br />

2 Uyole Agricultural <strong>Research</strong> Institute, Box 400, Mbeya, Tanzania


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

administered to 511 randomly selected farmers. Samples of local cowpea varieties and<br />

products prepared from cowpeas were collected for analysis in a laboratory at Sokoine<br />

University of Agriculture. The processing and preparation steps of cowpea recipes and<br />

products were documented.<br />

Proximate analysis of the samples<br />

The food samples for chemical analysis were grounded to pass through a one millimeter<br />

screen in a Christy and Norris 20cm laboratory hammer mill. Dry Matter percentage (%<br />

DM) was determined by drying the sample in an oven at 103 ºC – 105 ºC for 24 hours.<br />

Crude protein percentage (% CP) was determined by Kjeldahl method AOAC method<br />

No. 920.87 (AOAC, 1995) with the Kjeltec auto 1030 analyzer, whereby percentage<br />

Nitrogen obtained was used to calculate the % CP using the relationship: % CP = % N x<br />

6.25 (FAO, 1995). Ether Extract percentage (% EE) was determined using the Soxtec<br />

System HT- extraction technique AOAC method No. 922.06 (AOAC 1995) and<br />

percentage ash (% minerals) was determined immediately after the dry matter<br />

determination by incinerating the samples in a muffle furnace at 550 ºC for four (4)<br />

hours. The ash was cooled in a desiccators and then weighed. Crude fiber percentage (%<br />

CF) was determined by using the fiber system and Weende method (AOAC 1995).<br />

Nitrogen free extract percentage (% NFE) was calculated by differences as shown: %<br />

NFE = 100 - (% moisture + % CP + % EE + % CF + % Ash).<br />

Total minerals<br />

Total mineral content of the samples was carried out after dry ash. The ash was dissolved<br />

in a solution of 1:1 ratio of H2O : HCL, in which the concentration of the final mixture<br />

was 6NHCL. Total iron, zinc and calcium were determined by atomic absorption<br />

spectrophotometry by AOAC method 970.12 No. (AOAC, 1995).<br />

Data analysis<br />

Data for minerals and proximate analysis were entered in SPSS 12.0 for windows<br />

computer software. Descriptive statistics was used to determine the measures of central<br />

tendency for each analyzed cowpea variety. An analysis of variance of the results was<br />

done at 95% confidence interval (P≤0.05) using Tukeys Honestly Significant Difference.<br />

Homogeneity test was performed to determine homogenous sets.<br />

Results<br />

Demographic characteristics of farmers<br />

There were 240 interviewed farmers in Iringa of whom 124 (52%) were males and 116<br />

(48%) were females. In Dodoma 277 farmers were interviewed, 135 (49%) were males<br />

and 142 (51%) were females (Table 1). Farmer’s age ranged between 18 to 83 years with<br />

a mean of 45±15 years while in Dodoma the range was between 20 and 59 years with a<br />

mean of 35±7 years. In the two regions, the majority of the farmers had only studied up<br />

to class 7 (57% in Iringa and 88% in Dodoma) which is primary school level. However,<br />

5% and 1% of interviewed farmers had no formal education in Iringa and


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Dodomarespectively. About 81% of the households in Iringa and 67% in Dodoma had up<br />

to six family members.<br />

Table 1 Characteristics of interviewed farmers in Iringa and Dodoma<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Gender Male 124 51.7 135 48.7<br />

Female 116 48.3 142 51.3<br />

Education Class: 3-7 164 70.4 257 92.8<br />

Class 8-14 58 24.2 16 5.9<br />

No education 13 5.4 4 1.4<br />

Household size 1-6 members 192 80.8 183 66.8<br />

7-14 members 48 19.2 94 33.2<br />

Cowpea harvested, procured and utilization<br />

Most farmers (83% Iringa and 98% Dodoma) either grew and consumed their own<br />

cowpea, or obtained cowpea supplies from friends and relatives. Few of the farmers<br />

purchased cowpea for home consumption (Table 2). Farmers harvested an average of 75<br />

kg of cowpeas per household with a range from 53 to 118 kg and the amount sold per<br />

household ranged from 4 to 73kg with an average of 25kg per household. The amount<br />

kept at home for consumption ranged from 36 to 97 kg with an average of 52 kg. A<br />

substantial proportion of farmers prepared between 100 and 500 grams of cowpeas and<br />

between 100 and 1000 grams of cowpea leaves for household consumption depending on<br />

the household size. Per capital consumption ranged from 40 to 200 gm of cowpea grain<br />

for 84% of households in Iringa and 94% in Dodoma. At the same time per capital<br />

consumption for cowpea leaves ranged from 10 to 500 grams by 95% of households in<br />

Iringa and 100% in Dodoma, respectively.<br />

On days when cowpeas are cooked in a household, the same relish will be eaten with<br />

either rice or stiff porridge during lunch, dinner and with breakfast the following<br />

morning. More than 50% of households in Iringa and Dodoma consumed cowpea at least<br />

once and up to three times a week (Table 2). Cowpea leaves were also prepared and<br />

eaten on separate days. The leaves are consumed fresh especially during wet season and<br />

in dried form during the lean or dry season. Almost all farmers in the two regions<br />

consume cowpea leaves. Most cowpea recipes included; relish eaten with rice or stiff<br />

porridge (ugali), a mixture of dehulled maize and cowpeas (kande) and most frequently<br />

the cowpea buns (bagia).


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Table 2: Farmers Responses on Cowpeas Utilization<br />

Parameter Category Iringa Dodoma<br />

n % n %<br />

Ever eaten cowpea Yes 240 100 277 100<br />

How often eaten per day Twice 92 38.5 156 56.3<br />

Thrice 147 61.5 121 47.7<br />

How often eaten per week Once 77 32.1 28 10.1<br />

Twice 75 31.3 119 43.0<br />

Thrice 65 27.1 82 29.6<br />

Four Times 11 4.6 38 13.7<br />

Five Times 12 5 10 3.6<br />

Where the cowpea supplies are obtained Grow cowpea in my farm 124 51.7 131 47.3<br />

Purchase from the market 41 17.1 6 2.2<br />

Get from the neighbor 75 31.3 140 50.5<br />

How much cowpea is prepared in one meal 100-500gms 137 57.1 115 76.9<br />

500-2000gms 103 42.9 64 33.1<br />

How much cowpea leaves are prepared in one<br />

meal<br />

100-1000gms 146 61.9 227 89.7<br />

1000-4000gms 90 39.1 26 11.3<br />

Consumed green cowpea leaves Yes 240 100 277 100<br />

Consume dry cowpea leaves Yes 152 63.3 271 97.8<br />

Time of cooking green cowpea leaves 5-15 minutes 105 44.3 53 20.6<br />

20 – 90 minutes 133 65.7 214 79.4<br />

Time of cooking dry cowpea leaves 5-15 minutes 26 45.6 44 37.9<br />

20 – 90 minutes 31 54.4 72 62.1<br />

How leaves re prepared Chop-wash -cook 23 9.7 155 56.0<br />

Wash-chop-cook 214 90.7 44 44<br />

Cowpea soup with ugali or rice Relish 229 28.8 267 29.2<br />

Cowpea boiled with maize as kande Mixed with Maize 219 27.5 195 21.4<br />

Cowpea roasted 17 2.1 44 4.8<br />

Roasted milled to flour to prepare porridge Cowpea Porridge 28 3.5 66 7.2<br />

Milled to flour for preparing bagia Cowpea Buns 97 12.2 121 13.3<br />

Milled after soaking to prepare bagia Cowpea Buns 129 16.2 184 20.2<br />

Cowpea grain consumption 40-200gms 201 83.8 252 93.0<br />

201-500 39 16.2 19 7.0<br />

Cowpea leaves consumption 10 – 500 gms 224 94.9 247 100<br />

500 - 1400 12 5.1 0 0


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Content<br />

Minerals of nutritional importance that were analyzed in cowpea products included iron,<br />

zinc and calcium. Analysis showed that all varieties had significantly different (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Table 4: Mineral content of local cowpea grain and leaves from Iringa and Dodoma<br />

Moisture<br />

(%)<br />

Ash<br />

(%)<br />

Ca<br />

(mg/kg)<br />

Zn<br />

(mg/kg)<br />

Fe<br />

(mg/kg)<br />

Local Cowpea Dodoma 9.40 3.89 958.14 31.45 28.90<br />

Dehulled cowpeas Dodoma 10.28 4.32 363.78 28.00 25.02<br />

Cowpea Flour Dodoma 7.23 4.25 302.93 28.32 18.03<br />

Fresh Cowpea leaves Dodoma 5.59 6.28 1800.60 35.95 497.03<br />

Dry Cowpea leaves Dodoma 10.15 6.67 525.94 31.27 233.83<br />

Bagia Dodoma 35.35 3.01 893.08 13.67 16.34<br />

Local Cowpea Iringa 9.56 3.66 992.43 32.56 27.61<br />

Dehulled cowpeas Iringa 10.33 4.23 359.66 27.90 26.05<br />

Cowpea Flour Iringa 7.18 4.13 310.87 27.99 18.76<br />

Fresh Cowpea leaves Iringa 5.61 7.32 1809.63 36.13 499.52<br />

Dry Cowpea leaves Iringa 8.34 4.82 551.74 32.93 240.05<br />

Bagia Iringa 25.79 2.97 32.58 4.96 5.22<br />

Can we explain the difference from the two sites<br />

Proximate composition<br />

Proximate analysis showed that the cowpea varieties with the highest crude protein<br />

content were IT97K499-8 (26.12%) IT99K-7212-2-1 (26%) and Tumaini (25.95%)<br />

Varieties with lowest iron included IT00K-1207 (22.01%) and TZA 263 (22.6%). For<br />

fats, IT97K499-8 (11.18%), IT99K-7212-2-1 (10.98%) and Fahari (10.33%) varieties had<br />

the highest levels while the lowest included IT97K819-118 (8.17%) and IT96D-733<br />

(8.34%). Highest fibre was observed in TZA 263 (17.2%) followed by IT97K819-118<br />

(16.1%). The lowest in fibre were IT97K499-38 (12.34%), IT99K-7212-2-1 (12.53%)<br />

and VULI 2 (13.1%). Among the best new lines with regards to crude protein and fats<br />

content were IT97K499-8 and IT99K-7212-2-1.<br />

Table 5: Percent crude protein, fibre and content of 15 varieties of cowpeas<br />

Variety/Food component % DM %CP %CF %EE %NFE<br />

VULI 1 89.25 24.02 13.77 9.12 38.18<br />

IT99K573-1 88.92 23.32 14.51 8.36 38.85<br />

IT97K818-35 88.94 25.35 13.19 9.75 36.55<br />

IT96D-733 88.54 23.44 14.91 8.34 37.68<br />

IT89KD-288 88.93 24.08 14.23 9.11 37.64<br />

IT97K819-118 89.10 23.65 16.10 8.17 37.24<br />

FAHARI 89.29 25.46 13.45 10.33 35.86<br />

TUMAINI 89.13 25.96 13.83 9.89 35.23<br />

IT00K-1207 87.95 22.01 15.66 8.46 37.82


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

IT97K499-8 88.41 26.12 12.34 11.18 34.70<br />

TZA263 89.21 22.60 17.21 8.86 36.45<br />

VULI 2 89.53 25.64 13.10 10.25 36.53<br />

IT97K499-38 88.93 25.14 13.27 10.13 36.41<br />

B301 90.11 23.75 14.85 8.39 38.85<br />

IT99K-7212-2-1 89.68 26.00 12.53 10.98 35.89<br />

Local Cowpea 90.77 22.22 10.37 5.38 48.52<br />

Dehulled Cowpea 90.84 24.40 2.11 4.83 56.96<br />

Dehulled Cowpea Flour 87.75 22.53 1.11 2.07 54.30<br />

Cowpea buns (Bajia) Iringa 92.15 18.81 3.60 14.31 51.95<br />

Washed cowpeas for preparing buns 91.65 19.43 3.49 8.37 56.40<br />

Cowpea buns (Bajia) Dodoma 91.44 19.72 3.84 12.52 51.58<br />

Cowpea leaves 91.49 22.04 16.82 1.60 44.75<br />

In comparison with the improved lines and varieties, local cowpeas have comparable<br />

levels of dry matter (91%), crude protein (22%) and nitrogen free extracts (48%) (Table<br />

5). However, the levels of fats are relatively lower (5.4%) in comparison with the<br />

improved lines/ varieties ranging from 8% to 11.2%. A similar trend is shown with<br />

cowpea leaves. Dehulling the cowpeas reduces crude fibre significantly with a gradual<br />

fall in fats. With regard to the cowpea buns from Iringa and Dodoma regions there was<br />

significant drop in crude protein and crude fibre but a very significant rise in fats<br />

(14.3%).<br />

Discussion<br />

Macro and micro nutrient content<br />

In the two surveyed regions it was revealed that cowpeas are produced by majority of the<br />

farmers and consumed to a relatively large extent by all respondents, which provides an<br />

indication of cowpea importance in the two communities. Similarly, the survey revealed<br />

that cowpeas are prepared and consumed in a number of ways, such as relish for stiff<br />

porridge and rice, mixed and boiled with dehulled or un-dehulled maize and cowpea<br />

buns. In both regions, the cowpea buns were important as they were also a source of<br />

income in a number of households. The buns however, appear to have lower iron and<br />

calcium, especially those from Iringa region. The calcium, zinc and iron content of the<br />

cowpea buns were about 32.6, 5.0 and 5.2 mg/kg, respectively. The buns from Dodoma<br />

region had comparatively higher levels of calcium (893.1mg/kg), zinc (13.7mg/kg) and<br />

iron (16.3mg/kg). This difference might emanatre from the method of preparation of<br />

cowpeas in Iringa in comparison to Dodoma. In Iringa the grains are dehulled and soaked<br />

for about three hours and then washed in flowing river water for about an hour. This<br />

procedure of washing in flowing river water is not practiced in Dodoma. It is thought that<br />

these minerals are largely lost during washing.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

With regard to the deep frying of the cowpea buns, though some nutrients such as<br />

proteins and crude fibre were significantly reduced (from average of 22% to 19%<br />

protein), in the process fats were significantly increased (from average of 5% to 14%),<br />

while nitrogen free extracts and dry matter remained stable. The oil increase is attributed<br />

to the sunflower oil used to deep fry the buns. The farmers also cultivate sunflower for<br />

home use and sale. Greater oil intake is better as daily activities require adequate energy<br />

supply.<br />

Analysis of the developed cowpea varieties showed relatively high protein and fat in the<br />

levels of 24- 26% and 8%-11% respectively. Similar results have been observed by<br />

Horax et al., (2004) where they found cowpea seed contained 20-25% protein and was<br />

rich in essential and non-essential amino acids. Asante et al. (2006) were investigating on<br />

32 accessions of cowpea seeds, found protein and tannin contents ranging from 16.4-<br />

27.3% and 0.12- 2.38, respectively. In another study by Mgbemena et al. (2006)<br />

proximate analysis of various infested cowpea samples indicated increases in protein and<br />

crude fiber contents and decreases in moisture, ash, fats/oil and carbohydrates contents<br />

when compared with those of the uninfected samples. Percentage increases in protein and<br />

fiber contents ranged between 9.31-30.56%, and 1.24-15.11%, respectively while<br />

percentage decreases were, moisture (3.97-10.23%); ash (3.75-12.87%); fats/oil (1.43-<br />

10.00%); and carbohydrate (1.88-7.50%).<br />

Similarly the developed cowpeas were relatively high in calcium, iron, and zinc.<br />

However, considerable mineral variation was found among the analyzed cowpeas<br />

varieties. Similar study by Asante (2006) analyzing cowpea varieties found Ayiyi a cowpea<br />

variety with the highest calcium concentration of 2096.0 μg/g and Zn was detected in only few<br />

varieties ranging from 1501.0 μg/g to 2071.0 μg/g. Analysis of variance of the samples<br />

showed significant differences (p


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

With regard to cowpea leaves, it was observed that the leaves are consumed by almost all<br />

in the two communities surveyed. The young leaves are the ones normally picked,<br />

usually the first three or four from the cowpea plant. While some chop the leaves into<br />

small pieces before cooking, others boil the whole leaves. The leaves seem to have a big<br />

contribution with regard to calcium, iron and zinc. According to Schippers (1997)<br />

African Indigenous Vegetables (AIVs) play a highly significant role in food security of<br />

the under-privileged in both urban and rural settings. AIVs have traditionally taken an<br />

important role in African diets, being used both as medicine and as a vegetable. Many of<br />

these crops are highly nutritious, easy and cheap to grow.<br />

Results indicate that, there was twice as much calcium in leaves than in cowpea grains<br />

and almost 40 times as much iron. Similar results were observed by Weinberger et al.<br />

(2004) where indigenous vegetables showed high mineral levels. Since the iron and zinc<br />

levels in cowpea leaves have shown to be significantly higher than the levels in cowpea<br />

grains, creating more awareness and encouraging the utilization of cowpea leaves will<br />

contribute to alleviating micronutrient deficiencies from cheap sources especially in<br />

resource-poor families. However, it is also important to note that, vegetables that are<br />

common in a particular locality do not necessarily have values of iron contents that are<br />

comparable. For example, in a study by Weinberger et al. (2004) it was reported that<br />

cowpea leaves were identified and collected from four districts in Tanzania, their values<br />

were quite different varying from 179.0 mg in Kongwa, 66.0 mg in Singida, 77.5 mg in<br />

Muheza and 187.0 mg in Arumeru, per kg of edible portion. This is evident that the<br />

amount of minerals found in these vegetables does differ not only according to type of<br />

vegetable but also according to the place, location or district where they were obtained.<br />

This shows that the soil mineral content might have an influence in mineral uptake of the<br />

plants. This aspect can be further investigated.<br />

Adequacy of cowpea consumed in households<br />

As revealed in the results, the daily per capita consumption for the majority of the<br />

households surveyed ranged from 41 to 200 grams of cowpea, which means using the<br />

Atwater factors (4 kcal/g carbohydrates, 4 kcal/g protein and 4 kcal/g fats) an individual<br />

obtained about 135 to 658 kcal per day. Considering that a normal adult requires about a<br />

supply of 2500 kcal per day to perform moderate activities, the other amount of food<br />

eaten in conjunction with cowpeas such as rice or maize flour cannot fill the remaining<br />

gap. This shows that the amount of cowpeas consumed by most households does not<br />

satisfy daily requirements as the quantity eaten is inadequate. In the case of the developed<br />

varieties, which are higher yielding and resistant to Alectra, they will probably be<br />

accepted by the communities and the amount per capita of cowpeas consumed should<br />

increase provided they continue to cultivate the same area of land.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Conclusion<br />

Cowpea grain and leaves are widely consumed in the two surveyed areas. The<br />

contribution of micro and macro nutrients is significant for both developed lines and local<br />

varieties but with leaves having greater mineral content than grain. Therefore awarenessraising<br />

within communities to consume more cowpea leaves is required. However the<br />

amount produced per household is low compared to requirements. Consequently cowpea<br />

intake per capita is low, and does not meet the RDA. However, since households in both<br />

areas still have ample land they should be encouraged to increase the area planted as well<br />

as planting higher yielding varieties in conjunction with preferred local varieties. This<br />

does however require further market investigation.<br />

Other important nutrients such as vitamins, amino acid profile and levels of<br />

antinutritional factors (tannins and phytates) should also be further investigated. The<br />

vitamins and amino acids influence in nutrition while the antinutritional factors influence<br />

on the bioavailability of the nutrients in the body.<br />

Acknowlegement<br />

The authors would like to acknowledge the financial assistance from the <strong>McKnight</strong><br />

<strong>Foundation</strong>, who facilitated successful survey and laboratory analysis of macro and<br />

micronutrients.<br />

References<br />

Agazounon, C., Coulibaly, O. and Houndekon, V. (2004) Analysis of of cowpea<br />

processing techniques into ‘atta’ in Benin. Bulletin de la recherché Agronomique<br />

du Benin. No. 46, September 2004.<br />

Ahenkora, K. Adu Dapaah, H. K. and Agyeman, A. (1998) Selected nutritional<br />

components and sensory attributes of cowpea (Vigna unguiculata [L.]Walp)<br />

leaves Plant Foods for Human Nutrition 52: 221–229.<br />

AOAC (1995), Official methods of analysis. Association of Official Analytical Chemists<br />

methods, AOAC 16 th edition Nos. 963.13, 968.08, 970.12, 915.03, 920.87.<br />

Washington, D.C.<br />

Asante, I. K., Adu-Dapaah, H Addison (2006) Seed weight and protein and tannin<br />

contents of 32 cowpea accessions in Ghana. Journal of Tropical Science, 44<br />

(2): 77 – 79.<br />

Barret R. P. (1990) Legume species as leafy vegetables. In: Janick J, Simon J. E. (eds),<br />

Advances in new crops. Portland, OR: Timber Press, pp 391–396.<br />

Horax, R., Hettiarachchy, N. S., and Jalaluddin M. (2004).Preparation, characterization<br />

and functional properties of protein isolate from cowpea (Vigna unguiculata L.<br />

Walp.) Dept. of Food Science, Univ. of Arkansas, 2650 N. Young Avenue,<br />

Fayetteville, AR AR 72704, (2) Department of Agriculture, University of<br />

Arkansas, Pine Bluff, AR 71611.<br />

http://ift.confex.com/ift/2004/techprogram/paper_22759.htm.Accessed<br />

19.09.2009.


<strong>McKnight</strong> <strong>Foundation</strong> <strong>Collaborative</strong> <strong>Crop</strong>s <strong>Research</strong> Project No: 06-741<br />

Development and promotion of Alectra resistant cowpea cultivars for<br />

smallholder farmers in Malawi and Tanzania<br />

Mgbemena, I. C., Opara, F, N. and Iwuala, M. O. (2006) The effect of insect pest<br />

infestation on the nutritional composition of cowpea and maize. Department of<br />

Biotechnology, Federal University of Technology PMB 1526 Owerri, Nigeria<br />

International Journal of Natural and Applied Sciences 2 (2): 96-100, 2006.<br />

Mune Mune, M. A., Mbome, L. I. and Minka S. R. (2007) Improving the Nutritional<br />

Quality of Cowpea and Bambara Bean Flours for Use in Infant Feeding, Pakistan<br />

Journal of Nutrition 6 (6): 660-664<br />

Olaleke, A. M., Olorunfemi, O. and Akintayo, T. E. (2006) Compositional evaluation of<br />

cowpea (Vigna unguiculata) and scarlet runner bean (Phaseolus coccineus)<br />

varieties grown in Nigeria. International journal of food, agriculture and<br />

environment 4 (2): 39-43.<br />

Singh B. B., Sharma B. M. and Chambliss O. L. (1996). Recent advances in cowpea<br />

breeding. In Proceedings of the Second World Cowpea <strong>Research</strong> Conference, 5–8<br />

September 1995, Accra, Ghana. International Institute of Tropical Agriculture<br />

(IITA), Ibadan, Nigeria.<br />

Sinha, R. and Kawatra A. (2005) Effect of Processing on phytic acid and polyphenol<br />

contents of cowpeas (Vigna unguiculata (L) Walp. Plant Foods for human<br />

Nutrition, 58 (3): 1-8.<br />

Wang, N., Hatcher, D.W., Toews, R. and Gawalko, E.J. (2009) Influence of cooking and<br />

dehulling on nutritional composition of several varieties of lentils (Lens culinaris)<br />

LWT - Food Science and Technology, 42, (4): 842-848.<br />

Weinberger, K., John Msuya, J. and Mamiro, P. (2004) Domestication of selected<br />

African indigenous vegetables in Tanzania – an ex-ante impact assessment.<br />

AVRDC – The World Vegetable Center, pp22.

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!