Checklist - ARCHIVE: Defra

INTRA-COMMUNITY TRADE IN BOVINE EMBRYOS – CHECKLIST
ITAHC No: ……...………………………...……………..
This checklist should be completed after reading the relevant Intra-trade animal health certificate
(ITAHC) and BOVINE-EMBRYO-NFG.
Part II of the ITAHC may be signed if the answers to ALL the questions in Section A and either
Section B or Section C (as appropriate) are YES.
SECTION A – Consignment details
1 Consignor (name and full address):
2 Consignee (name and full address):
3 Place of loading:
4 Name and address of embryo collection team:
5 Means of transport:
Seal number on cryogenic vessel:
6 Place and Member State of destination:
7 Registration number of embryo collection team:
8 Number and code-mark of embryo containers:
(identification details of donor dam and donor sire)
9 Identification of consignment:
SECTION B – For embryos produced in the UK
Part I - DONOR COW YES NO
1 Do you have a BOVINE EMBRYOS-OED veterinary support certificate duly
and properly completed for the donor cow(s), and were they valid at the time
of collection of the ova/embryos?
BOVINE-EMBRYOS-CKL (29/08/2007)
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2 Are you satisfied that the BOVINE EMBRYOS-OED veterinary support
certificate relates to the donor cow(s) and, as far as can be determined, is
factually correct? In particular, that the donor cow was on a holding outside
the protection/surveillance zones established following the confirmation of an
epizootic disease (e.g. FMD) to which cattle are susceptible, at the time of
collection.
3 Were the embryos for export collected, processed and stored by a Collection
Team approved by DEFRA/SEERAD/NAW at the time of the collection?
4 At an examination carried out on the day(s) of collection were the donor cows
free of clinical signs of infectious or contagious disease?
5 Were the embryos processed (examined, washed, treated and placed in
identified and sterilised containers) in a laboratory facility (either permanent or
mobile) which was not in a zone subject to prohibition or quarantine
measures?
6 Have the embryos been washed at least 10 times according to the standards
laid down by the International Embryos Transfer Society (IETS)? In particular:
- did the washing include Trypsin?
- were only embryos from the same donor may be washed at the same time?
- was the zona pellucida intact before and after washing?
- after washing, was the zona pellucida of each ovum/embryo examined over
its entire surface under a magnification of at least 50x and found to be intact
and free from adherent material?
7 Was the medium used for the collection, processing (examination, washing,
treatment), conservation / freezing of the ova/embryos sterilised in
accordance with IETS protocols and was it handled in such a way that its
sterility was maintained?
8 Have antibiotics been added to the collection, washing, and conservation
medium?
9 Following collection and examinations as at 6 above, were the ova/embryos
placed in sterile ampoules/straws which were duly marked with the date of
collection, the breed, the identity of the donor and the name and/or the
number of the collection team?
10 Does each ampoule/straw contain embryos from one female donor only?
BOVINE-EMBRYOS-CKL (29/08/2007)
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YES NO

11 Were the ampoules/straws sealed at the time of freezing (in fresh alcohol or
liquid nitrogen), and were they then placed in disinfected containers,
containing fresh liquid nitrogen?
12 Following collection/production, were the ova/embryo stored in approved
stores (rooms) in which nothing else has been stored other than bovine
embryos and bovine semen eligible for intra-community trade?
13 Were the ova/embryos placed in transport flasks which were cleaned and
disinfected or sterilised before use and which were sealed and numbered with
the corresponding export health certificate number prior to despatch from the
approved facilities?
Seal number: …………………………………………………………………….
BOVINE-EMBRYOS-CKL (29/08/2007)
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YES NO
Part II – DONOR SIRE YES NO
1 Do you have a fully completed BOVINE EMBRYOS-CKA certificate in respect
of the semen used?
SECTION C – For embryos imported into the UK
Answer either Question 1 or Question 2, as appropriate YES NO
For Embryos Originating from EU Member States
1. Have you seen an Intra-Trade Animal Health Certificate confirming that the
embryos were legally introduced into the UK from another EU member state?
ITAHC No: ……………………………………………………………………….
For Embryos Imported from Third Countries
2. Have you seen a certified copy of the veterinary health certificate and the
Common Veterinary Entry Document for products (CVEDP) confirming that
the embryos were legally imported into the UK?
AND
Does the certificate conform to one of the following models laid down under
Commission Decision 2006/168/EC:
either:
(a) Annex II – for in-vivo derived embryos of domestic animals of the bovine
species for import, collected in accordance with Council Directive
89/556/EEC?
or:
(b) Annex III – for in-vitro produced embryos of the domestic animals of the
bovine species for import, conceived using semen complying with
Council Directive 88/407/EEC?
Certificate No: ………………………………..…………………………………….
CVEDP No: ……………………………………………..………………………….

Date....................
BOVINE-EMBRYOS-CKL (29/08/2007)
Signature...…………………………….............
..............
Name......…………………..…...……............................
Official stamp (in block capitals)
Team veterinarian
Address ..............…………………………….................
.....................………………………................
PLEASE NOTE:
THIS SUPPORT CERTIFICATE SHOULD BE RETAINED BY THE OFFICIAL VETERINARIAN AND
MUST NOT ACCOMPANY THE CONSIGNMENT TO ITS FINAL DESTINATION.
Department For Environment, Food and Rural Affairs,
1A Page Street,
London
SW1P 4PQ
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