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The Genetics of Glucosamine Resistance in Yeast J. J. Elliot B.Sc. Department of Biological Sciences (Submitted in partial fulfillment of the requirements for the degree of Master of Science) BROCK UNIVERSITY St. Catharines, Ontario, L2S 3A1 August 1975
Page 5: Abstract A preliminary genetic anal
Page 9: - 4 - Table of Contents _ Page Intr
Page 13: - 6 - List of Tables Title Page Tab
Page 17: Nam et ipsa scientia potestas est.
Page 21: 10 - Most accomplishments to date i
Page 25: - 12 - oxidation by dinitrophenol (
Page 29: - 14 - sporadically risen and waned
Page 33: -a 1 3 Q 0) -a 8 Q Q. -16a- m 70 I
Page 37: - 17 - perhaps related to mitochond
Page 41: 18 C. The Life Cycle and Genetics o
Page 44: Figure 2. The Life Cycle of Sacchar
Page 49: - 22 energy metabolism" or aem have
Page 53:
- 24 shown that suppressive petites
Page 56 and 57:
' •
Page 59:
- 26 - strate the existence of pola
Page 63 and 64:
- 29
Page 65:
X) 2 -30- 3 CO
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Yeast Strains - 32 - CHAPTER 2 MATE
Page 73:
SM: - 34 - Supplement mg/1 histidin
Page 77:
Micromanipulation and Tetrad Analys
Page 81 and 82:
Phase I - Preliminary Screening - 3
Page 83:
esponses. More than half of the dip
Page 86 and 87:
Plate I. Growth responses of diploi
Page 88 and 89:
7A *t'
Page 90 and 91:
Figure 4. Complementation Pattern.
Page 93:
- 44 - patterns of mitochondrial er
Page 97:
- 46 Table II. Vegetative Segregati
Page 101 and 102:
- 48 -
Page 103:
Fig.5 RES COLONY -49- .P//DI SENS -
Page 107:
Table III. Tetrad Analysis. 51 - Nu
Page 111 and 112:
- 53
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Fig. 6 - 54 GR6 GR7 J 4 months stor
Page 116 and 117:
Figure 7. Vegetative Segregation of
Page 119:
Phase II - Detailed Analysis 57 - A
Page 122 and 123:
< < >
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- 60 - Vegetative segregational dat
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* v • - 61 -
Page 131:
- 63 - No resistant spores were rec
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- 65 analysis (6L4) which also yiel
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Figure 9. Vegetative Segregation of
Page 141:
Enrichment of Resistance in Diploid
Page 145 and 146:
- 70 - CHAPTER 4 DISCUSSION Glucosa
Page 147:
- 71 - specifically and therefore n
Page 151:
73 - constituting the mitochondrial
Page 154 and 155:
' ' '
Page 157 and 158:
- 76 - While the above hypothesis m
Page 159:
- 77 - demonstration of G segregant
Page 163 and 164:
- 79 - resistant and wild type (4B2
Page 165:
- 80 - In contrast, drug resistance
Page 169:
- 82 - R S Alteration of this G /G
Page 173 and 174:
- 84 - mtDNA and therefore was with
Page 175:
- 85 - Though the EB treatment used
Page 179 and 180:
87 The results from the reverse cro
Page 181:
location of G in strain GR6. - 88 R
Page 184 and 185:
- Ifid site. t la
Page 187:
- 90a - ADDENDUM It has been sugges
Page 191:
- 90c - LINNANE, A.W., C.L. BUNN, N
Page 194 and 195:
' i snvA ,!VA rL9S
Page 197:
- 93 - Ebner, E., L. Mennucci and G
Page 200 and 201:
' ' ilsU IsfiF weH 39H
Page 202 and 203:
' .A ,>f U '.
Page 204 and 205:
- '
Page 207 and 208:
- 98 Rank, G.H. 1970. Genetic evide
Page 209:
- 99 - Sherman, F. and J.W. Stewart
Page 213 and 214:
101 -
Page 215:
Appendix I(Q ) Linkage Group w -102
Page 219 and 220:
- 104
Page 221:
(0 < < O O O o o I ° s N ^ 2 o o o
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