Real time PCR-serotyping oligonucleotides
Real time PCR-serotyping oligonucleotides
Real time PCR-serotyping oligonucleotides
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
CR931683<br />
(wzy )<br />
CR931685<br />
(wzy )<br />
CR931702<br />
(wzy )<br />
8626-8711<br />
9049-9274<br />
23A-F<br />
23A-R<br />
23A-Probe<br />
23F-F<br />
23F-R<br />
23F-Probe<br />
33F/33A/37-F<br />
33F/33A/37-R<br />
33F/33A/37-Probe<br />
CTCCCCTCCATTACCCATTTGG<br />
TGAAGAAAGTGCTGTTTGTGAACC<br />
AGCTAGAAC”T”CCCACACTCCCTACTCCCA ROX/CY5 5<br />
GACAGCAACGACAATAGTCATCTC<br />
TCCATCCCAACCTAACACACTTC<br />
ATTGTGTCCA"T"AACCCTTCGTCGTATTTCCAAAG<br />
GGAACTGGTTCAGCAACTATACG<br />
GGTTCTAAGACCGTCTGAAATACC<br />
CCCCAAATAGGAC"T"TTTCTGCCATGCCAAA<br />
ROX/CY5 5<br />
"T" 4 =BHQ2 BHQ2/BHQ3<br />
"T" 4 =BHQ2<br />
11778-11882<br />
4<br />
HEX<br />
"T" =BHQ1<br />
1- Abbreviations: FAM, 6-carboxyfluorescein; HEX, hexachloro-6-carboxyfluorescein; ROX, 6-carboxy-X-rhodamine; CY5, indodicarbocyanine; BHQ, black hole quencher.<br />
2- Locked nucleic acid nucleotides are underlined<br />
BHQ2/BHQ3<br />
3- The probe for serotype 2 is labeled with FAM for the triplex reaction formulated for the Africa and Latin America region(s) and HEX for the triplex reaction formulated for the USA and Asia regions; the probe for<br />
serotype 5 is labeled with FAM for the triplex reaction formulated for the USA and Asia regions and HEX for the triplex reaction formulated for the Africa and Latin America region(s) reaction set.<br />
4-"T"= black hole quencher placed internally on the thymidine base<br />
5- Probe is labeled with CY5 if ROX is used as a reference dye<br />
BHQ1<br />
200<br />
200<br />
100<br />
300<br />
300<br />
200<br />
200<br />
200<br />
200
Change language