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The Tell-Tale Stain

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New Mycobacterium species from 2 patients who died of diffuse<br />

lepromatous leprosy (DLL).<br />

DNA extraction from heavily infected freshly frozen liver tissue or<br />

paraffin-embedded skin tissue<br />

Six genes of the organism were sequenced.<br />

Significant genetic differences with M. leprae were found, including a<br />

2.1% divergence of the 16S ribosomal RNA (rRNA) gene,<br />

<strong>The</strong> unique clinicopathologic features of DLL led them to propose<br />

Mycobacterium lepromatosis sp nov.

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