FORMATO EUROPEO PER IL CURRICULUM VITAE

E UROPEAN
CURRICULUM VITAE
ET STUDIORUM
1. PERSONAL
INFORMATION
Name ANNA ALFIERI
First Home address P.zza FedericoII N°6 – 00040 Pomezia (RM)
Telephone number +39 338-7973687 +39 6-9120703
E - mail anna_alfieri@alice.it
Nationality Italian
Date of birth 05, August 1969
Family status Divorced
2. WORK
EXPERIENCE
• Date Since February 1997
• Name and address of
employer
• Type of business or
sector
IRBM Institute of Research Molecular Biology– Via Pontina Km 30.600
00040 Pomezia- Italy
Preclinical Drug Metabolism and Pharmacokinetics department
• Title Analytical Chemist – DMPK group
• Main activities and
responsabilities
• Pharmacokinetics Studies – quantitative analysis of biological
samples.
• Development, optimization and validation of bioanalytical
methods by LC/MS/MS for qualitative and quantitative
analysis of small molecules.
• Automated samples preparation by liquid handling robotic
system and Solid Phase Extraction, data elaboration and
preliminary evaluation of pharmacokinetic profiles (PdPK
LIMS).
• Plasma and blood stability studies
• Physical-chemical measurement (e.g. LogD, LogP, Solubility,
CHI)
• Purity Check of final synthetic compounds (Purity Index) by
HPLC/DAD/LC and LC/MS Ion Trap Mass Spectrometry.
• Enantiomeric Resolution by HPLC – Highthrouput column
screening and semi preparative chiral separations.
• Diastereoisomeric mixtures separation.
• Date (from-to) 1989/1997
• Name and address of
employer
Menarini Ricerche – Via Tito Speri n°10, 00040 Pomezia (RM)-Italy
• Type of business or
sector
Medicinal Chemistry Department
• Title Staff Organic Chemist
• Main activities and
responsabilities
Syntesis of new antitumoral Distamycin and Antracycline derivates.
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• Date (from-to) 1988/1989
• Name and address of
employer
Azienda Sanitaria Locale RM12– V.le Di Tor Di Quinto – Rome Italy
• Title Lab Techinician
• Main activities and
responsabilities
Clinical Analysis of Human blood, urine and feces
3. EDUCATIONAL
BACKGROUND
• Date June 1988
• Secondary School Istituo Tecnico Industriale Statale G.L. Bernini (Statal Industrial
Technical Institute “G.L. Bernini”, High school)
• Title of qualification
• Training Experiences
and Courses
Perito Chimico industriale, indirizzo chimico clinico sanitario
(Industrial Expert and Technical Leader in Sanitary Clinical
Chemistry)
• Date June 1992, Gargnano (BS)
• School Societa' Chimica Italiana SCI (Italian Society of Chemistry)
“XVII Seminari di sintesi in chimica organica "A. Corbella" (17th
Seminars in Organic Chemistry "A. Corbella")
• Date June 1994, Gargnano (BS)
• School Societa' Chimica Italiana SCI (Italian Society of Chemistry)
“XIX Seminari di sintesi in chimica organica "A. Corbella" (19th
Seminars in Organic Chemistry "A. Corbella")
• Date August 1995, Milano
• School Societa' Chimica Italiana SCI (Italian Society of Chemistry)
XVIII Congresso Nazionale Formazione, Ricerca e Innovazione (18th
National Congress on Formation, Research and Innovation)
• Date March 1996, Norwich
• School School of Sciences - University of East Anglia, Norwich
• Date June 2000, Roma
“Short Course in Organic Chemistry.”
• Training Waters Technology Seminar
“HighThroughput Analysis and purification strategies for drug
discovery.”
• Date March 2001 Vimodrone (MI, Italy)
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• Training Waters Technology Seminar
“HPLC-MS”
• Date July 2001, Roma
• Training Stepbio Technology Seminar
• Date October 2001, Roma
“Chiralita’ presente e futuro” (Seminar on Chirality)
• Training Waters Technology Seminar
• Date May 2004 Rome
“Fast and Ultra Fast Chromatography”
• Training Waters Technology Seminar
“UPLC Course”
• Date May 2005 Rome
• Training Waters Technology Seminar
• Date November 2006 Rome
“Solution for Pharmaceutical Applications”
• Training Stepbio Technology Seminar
• Date January 2008 Rome
• Training Agilent Pharma Seminar
4. LANGUAGES
FIRST LANGUAGE ITALIAN
OTHER LANGUAGES
"Separazioni Chirali su Fasi Chirali da PIRKLE ad oggi" (Chiral
Separation by means of PIRKLE Chiral Stationary Phases)
" Soluzioni per l'analisi farmaceutica" (Solution for Pharmaceutica
Analysis)
ENGLISH
• Reading skills GOOD
• Writing skills GOOD
• Verbal skills GOOD
5. PROFESSIONAL SKILLS
AND COMPETENCES
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During the last twenty years I acquired a broad ownership in bioanalytical methodologies and
scientific knowledge in the support of preclinical and clinical bioanalysis. Some of my skills and
knowledge include:
• High capacity qualitative and quantitative LC-MS/MS analysis on triple quadrupole, ion trap
mass spectrometers and QTof.
• Robotic automation for high throughput sample analysis
• Fully integrated bioanalytical LIMS system
• Chiral Separation
• Organic Synthesis
• Physical-chemical measurement
6. TECHNICAL SKILLS
AND COMPETENCES
Experienced knowledge in the use of the following laboratory instruments:
• Mass Spectrometer Systems, e.g. API2000, API3000, API4000, API5000, Ion Trap coupled
with HPLC pumps and CTC Pal Autosampler
• HPLC Systems, e.g. Perkin-Elmer ,Waters, Agilent and Jasco Systems
• Detectors, e.g. Diode Array, Light-scattering
• Chip based ESI infusion system, i.e. Triversa NanoMate
• Liquid Handler Systems for high-throughput sample preparation (Plasma Protein Precipitation,
SPE and Filtration); e.g. Hamilton StarPlus; MultiPROBE II Plus HT and Tecan System
Ability to troubleshoot instrumentation/method problems as needed to ensure their proper
functionality. Capabilities in setting work flows/standards and in assuring that they are properly
followed for the timely and proper conduct of the study.
7. COMPUTER SKILLS
AND COMPETENCES
Perfect command of Windows XP Operating Systems;
Good command of Microsoft Office tools (Word, Excel, PowerPoint);
Capability to use the following software:
ISIS application for database; Bibliographic research web tools (PubMed, ScyFinder); data elaboration
software (Analyst, Xcalibur, MassLynx,Empower) and bioanalytical LIMS system (Watson LIMS) for PK
calculation (PDM PKLIMS).
8. PERSONAL SKILLS
AND COMPETENCES
Team spirit and ability to engage in a dialogue with key colleagues to identify opportunities and user
needs and translated them in specific actions.
Good ability to adapt to new multicultural environments acquired by working in a large multinational
industry during the last years and interacting with international co-workers; Good communication
skills.
9. PUBBLICATION AND
COMMUNICATION TO SYMPOSIA
Gardelli,C.; Muraglia, E.; Crescenzi, B.; Ferrara M.; Orvieto, F.; Pace, P.; Pescatore, G.; Poma, M.;
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Rico Ferreira, M.; Scarpell, R.; Hominck, C. F.; Ikemoto, N.; Alfieri, A; Verdirame, M.; Bonelli ;
Gonzalez Paz, O.; Taliani, M.; Monteagudo, E.; Pesci, S.; Laufer, R.; Felock, P.; Stillmock, K.;
Hazuda; D.; Summa, V.
"Discovery and synthesis of HIV Integrase Inhibitor : Development of potent and orally
bioavailable N-methyl pyrimidone"
V.J.Med. Chem. (2007), 50, 4953-4975.
P.Felicetti, F.Arcamone, F.Animati, C.Rossi, A.Alfieri, A.Ciucci, M.Bigioni, P.Lombardi.
" Sintesi di distamicine antitumorali dotate di selettivita' di sequenza verso il DNA".
XXII Convegno Nazionale della divisione di chimica organica-Viareggio 1994.
A.Alfieri, F.Animati, F.Arcamone, P.Felicetti, G.DiPietro, G.Giannini, P.Lombardi, C.Rossi, .
"Synthesis and antitumoural activity of novel distamycin derivates".
II Congresso Congiunto Italo-Spagnolo di chimica Farmaceutica-Ferrara 1995.
A.Alfieri, M.Verdirame, A.Pessi, F.Bonelli.
" High throughput analysis of combinatorial libraries by high speed HPLC-MS".
Norcia 1998
M.Verdirame, A.Alfieri, F.Bonelli
“Turbolent Flow Chromatography in Early Drug Discovery and preclinical studies “
16th International Bioanalytical Forum - Guilford 4 July 2005
Gonzalez Paz, O. ; Taliani, M.; Di Marco, A. ; Monteagudo, E.S. ; Bonelli,F. ; Fiore,F. ; Fonsi,M. ;
Naimo,F. ; Verdirame,M. ; Alfieri,A. ; Marcucci,I. ; Summa,V. ; Rowley,M ; Laufer,R.
“ Preclinical pharmacokinetics and metabolism of MK-0518, a potent HIV Integrase
inhibitor in phase III clinical trials”
AIDS 16 th Internation Conference, Toronto, Ontario, Canada – 13 August 2006
Verdirame,M. ; Alfieri, A. ; Di Marco, A. ; Monteagudo, E.S. ; Laufer,R. ; Bonelli,F.
“ Application of TFC_LC/MS/MS for the rapid analysis of hepatocyte metabolic stability
samples”
Liquid Chromatography/ Mass Spectrometry 23 rd symposium, Montreux, Switzerland, 08 November
2006
Massimiliano Fonsi, Maria Vittoria Orsale, Anna Alfieri, Maria Verdirame, Fabio Bonelli, Fabrizio Fiore,
Massimo Aquilina, Ralph Laufer, Edith Monteagudo and PARP team.
"DMPK support for the identification of a PARP-1/2 inhibitor. Evolution from L-001818680
to the PCC candidate L-001946812"
Filocamo, G.; Altamura, S.; Alfieri, A.; Bala, G.; Bolkovec, J.T.; Biasol, G.; Brunetti, M.; Colaceci,
F.; Cecchetti, O.; Demuth, T.; Fiore, F.; Fonsi, M.; Gonzales Paz; O.; Hardwich, J.; Jones, P.;
Lauring, B.; Mesiti, G.; Monteagudo, E.; Nardi, M.g.; Orsale; M.; Paolino; C.; Sasso; R.; Zucconi;
A.; Steinkuler, C.; Gallinari,P.
"Taegeting Shh patway for anti-cancer therapies"
Poster Session IRBM Program Review – Merck Research Laboratories – Via Pontina Km 30600
Pomezia – 5 May 2008
"Discovery and Synthesis of HIV Integrase Inhibitors: Development of Potent and Orally
Bioavailable N-Methyl Pyrimidones"
Gardelli, C.; Nizi, E.; Muraglia, E.; Crescenzi, B.; Ferrara, M.; Orvieto, F.; Pace, P.; Pescatore, G.;
Poma, M.; Rico Ferreira, M.; Scarpelli, R.; Homnick, C. F.; Ikemoto, N.; Alfieri, A.; Verdirame, M.;
Bonelli, F.; Gonzalez Paz, O.; Taliani, M.; Monteagudo, E.; Pesci, S.; Laufer, R.; Felock, P.;
Stillmock, K. A.; Hazuda, D.; Rowley, M.; Summa, V.
J. Med. Chem.; (Article); 2007; 50(20); 4953-4975
For references please contact : Dott.ssa Edith Monteagudo (IRBM) e-mail:
edith_monteagudo@merck.com
Dott. Fabio Bonelli (IRBM) e-mail : fabio_bonelli@merck.com
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I hereby grant permission to use my personal data in accordance to the Italian Law D. LGS. 196/2003
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