Cell Respiration Bean Lab

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$Summer 2013 Math Institute Brochure - Carrollton-Farmers Branch ...$

Nome Dote AEROBTC CELLULAR RESPTRATTON (BEAN LAB) fn this experiment, you will compore the oxygen consumption of nongerminoting soybeons ond genminoting soybeons. Oxygengos is consumed during oerobic cellulor respirotion ond corbon dioxide gas is given oIf. The uptoke of oxygen is the evidence thot on orgonism is corrying on oerobic cellulor respirotion. Your doto will not be affected by the releose of corbon dioxide becquse you will use potossium hydroxide (KOH) to remove it. Note the following eguotions fon: the removol of COz6Y KOH is' 6Q, + ?KOH ---------2 KzCOt + HzO oerobic cellulor respirotion ist C5HpO5+ Oz (Q2 + ll2O + ATP EXPERIMENTAL PROCEDURE -_2 1 - Prepore o room temperoture woter both: Fiff o green tub to q level of -5 centimeters of woter. Attoch mosking tGPe ocross the woter both, moking q "sling" to hold the respirometer pipette ends out of the water (see Figure b)' 2 - Construct three respirom elers (see Figure o): Obtain three test tubes, eoch with o stoPPer ond calibroted pipette' Lobel the tubes 1, 2, ond 3 with the greose pencil in your desk drawer' 3 - Follow all the remoining directions CAREFULLy! place oneof the six smoll wods of obsorbenf cotton in the bottom of each test tube. Carefully dispense drops of 15%KOH (potossium hydnoxide) to saturote the cotton in the tubes (fully wet, but not so99y). Do not get ony KOH on the sides of the test tubes; the tube 4gg! be dry. Reploce o tube with o clean one if you occidentolly get the sides wet. ploce o smoll wod of dry cotton on top of eoch of the KoH-sooked cotton wads. Use the some qmounts of cotton ond KoH for eoch respirometer. 4 - Obtoin o 100 ml groduoted cylinder ond odd 50 ml of woter' Drop in twenty germinoted soybeons ond meosure the omount of woter disploced (thot is, how much the woter rises qbove the 50 ml mork)' Record this number here: ml. Remove the woter ond germinoting beons. Gently dry the beons on o Poper towel. 5 - Plqce the germinoting beons in tube 1. fnsert the stoPper fitted with o colibroted pipette (seeFigure a)'
tube 1 germinatrng bears, no gravel Figure a tube 2 nongermlna ting bears & gravel tube 3 aqua.riu.Er gravel d.I-t cotton KOH soaked cotton Figure b . Respiromeiers in the !'/ater bath' Beon lab P.2 masking lape sllng 6 - Again, add 50 ml of woter to the 100 ml groduoted cylinder. This time, drop in tweniy nongerminoting soybeons. Add oguorium grovel until the some omount of woter is disploced os in step 4. Dry the gravel ond beans on o paper towel. 7 - Place the nongerminoting beons and the grovel in tube Z. fnsert the stopper fitted with o colibrated pipette. 8 - Once more, odd 50 ml of water to the 100 ml cylinder. Then, drop in grovel uniil the some omount of water is disploced os in step 4. Remove the woter qnd gravel. Dry the grovel on o poper towel. 9 - Placethe grovel in tube 3. fnsert the stopper fitted with o colibroted pipetle. 10 - Using the test tube tongs, loy tha three respirometers in the woter bqth, with the pipettes resiing on the mosking tope sling (seeFigureb). Qently set the weight opparotus ocross the three tubes to submerge them. Woit 7 minutes so the temperature of the tubes ond the woten is the some. 11 - Remove the sling. Using the test tube tongs, position the three respirometers ond the weight dpporotus dtagonallyin the tub so thot they loy entirely under water ond so the pipette colibrotions con be eosily read (keep those warm fingers out of the woterl). Water will enter the pipettes fon o short distonce ond then stop. ff woten continues to move into o pipette, check for leoks in the respiiomefer (with fingernoil, try gently pushing down the waxy parafiln thot seols the pipette to the stopper hole. If this doesn't correct the problem, exchange your pipette stopper for onother from the cort.) pipetl tip
Page 1 and 2: Cellular Respiration Lab - The Bean
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Cell Respiration Bean Lab

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