BAY ZOLTÁN - BAY-LOGI

BAY ZOLTÁN
Intellectual Properties
What is it?
L - Malic Acid
The malic acid is one of the hydroxy acids that are produced by aerobic
organisms in the so called citric acid cycle. It is synthesized by the enzyme
fumarase, that incorporates a water molecule into fumarate resulting in a
malate molecule that now has an asymmetric carbon atom. Because of this
asymmetry, malic acid has two forms, - called optical isomers, L(-) and D(+) -
that could be distinguished only by their optical behaviors. Chemical
synthesis produces both optical isomers in equal amounts while biosynthesis
produces the L(-) form only. It is important to note, that this fact cause a limit
of the economical useage of DL malic acid, because the living organisms are
able to utilise the L(-) isomer only.
Proprietary
The intellectual property is owned 50% by the Biotechnology and 50% by the
Logistics and Production Engineering Institute of the Foundation.
Intellectual Protection
The bioreacor and the enzymatic conversion is protected in Hungary under
the patent number of P9602431 / 220588 which is valid until 2016.09.05. The
technology has a know how documentation.
Product description
Na-malat and Na-fumarate 80:20 solution or solid product.
L-malic acid 99 % purity.
Enzyme immobilization in pearls.
Controllable bioreactor with a self developed polarimeter.
Advantages of the technology
The technology do not have significant improvement comparing to other malic
acid production technology but it realized on a different way of production.
The recen technology is capable for producing 6 liter / hour 1.2 mol Na-malat
and 5 kg / day L-malic acid.
Products
L-malic acid and
Na-malat
Immobilized enzymes
in pearls
Controllable bioreactor
Polarimeter

Innovation directions
1. Including electrodialysis into the pilot plant scale
technology to decrease the production cost.
2. Improve quality of L-malic acid for
pharmaceutical production.
3. Implement a technology of L-mailc acid for
biopolymer production.
4. Production different malic acid salts.
Pilot Plant scale bioreactor and film dryer
Ion exchange vessels
Contact point:
Dr. Norbert Babcsán, project manager
BAY-LOGI
3519 Miskolctapolca, Iglói út 2., Hungary
Tel.: +36 46 560 120,+36 30 415 0001
Fax: +36 46 422 786
babcsan@bzaka.hu, www.bayzoltan.org
Technology description
For ensyme production we have isolated the gene
coding for the enzyme fumarase and cloned it into
a plasmid that has suitable genetic signals for
overproduction of the enzyme. The bacterial strain
carrying this plasmid produces huge amounts of
the catalytic enzyme. This microorganism was
immobilized into a specially developed ion resistant
molecular composite biopolymer matrices. After
immobilization the cells were killed. In addition a
special coating was applied to the particles that
prevents even the dead cells and macromolecules
from escaping to the environment. These particles
were used to create a continuous flow bioreactor
that converts fumarate to malic acid possessing
extremly high half life time.The reactors are not
produce bypass products eq. amino acids,or other
organic acids from the substrate.We scaled up the
bioreactor technique to industrial level. The
various parameters affecting on the efficiency of
malate production were optimized.
1.5 ton Na-malat has been produced