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Volume 23 Number 1 - Wing Beats - Wing Beats - Florida Mosquito ...

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Public PowerPoint Presentations and Pupae by Adrian L Salinas<br />

One of my duties at the Collier<br />

<strong>Mosquito</strong> Control District in<br />

Naples, <strong>Florida</strong>, is to rear Aedes<br />

aegypti for public educational<br />

events. I learned how to rear<br />

mosquitoes years ago from<br />

Johnny Jackson at the USDA<br />

Center for Medical and Veterinary<br />

Entomology Laboratory, in<br />

Gainesville, from Mike Greer at<br />

the Public Health Entomology<br />

Research and Education Center<br />

in Panama City, and by reading<br />

the Manual for <strong>Mosquito</strong> Rearing<br />

and Experimental Techniques,<br />

published by the AMCA. During<br />

community events, we discuss<br />

the mosquito life cycle and use<br />

live mosquito larvae for school<br />

science programs. PowerPoint<br />

presentations, classroom<br />

activities, and information<br />

booths are great, but adding<br />

live wigglers always gets<br />

everyone’s attention. A few days<br />

before each event, I hatch the<br />

mosquito eggs, sprinkle the<br />

water with food and then watch<br />

them grow. For school projects,<br />

such as a bioassay, I use second<br />

to third instar larvae or I may<br />

need all the stages for a public<br />

information event. Also, rearing<br />

to replenish egg stock is a vital<br />

ongoing process. One of the<br />

more interesting times of the<br />

rearing process is anticipating<br />

the change from the fourth<br />

instar to pupae. What I work<br />

towards is a high percentage,<br />

synchronized change to pupae.<br />

I get really excited when I go to<br />

the insectary and see trays of<br />

pure pupae, as pictured above.<br />

So, the next time you see pupae,<br />

think of me pouring thousands<br />

into a sieve, with a smile on<br />

my face, as I put them into the<br />

rearing cages.<br />

Adrian L Salinas<br />

Public Information Officer<br />

asalinas@cmcd.org<br />

Collier <strong>Mosquito</strong> Control District<br />

600 North Road<br />

Naples, FL 34104<br />

<strong>23</strong>9-436-1000<br />

<strong>Wing</strong> <strong>Beats</strong> Spring 2012 15

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