XTN™TALEN Programs & Pricing Link - Transposagen ...
XTN™TALEN Programs & Pricing Link - Transposagen ...
XTN™TALEN Programs & Pricing Link - Transposagen ...
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XTNTALEN <strong>Programs</strong> & <strong>Pricing</strong> <strong>Link</strong><br />
All programs include:<br />
• Free Feasibility Study – non-binding <strong>Link</strong><br />
• Custom design- submit either the DNA sequence or gene symbol and species<br />
• Human, mouse, rat & zebrafish controls & GFP reporter verification system<br />
controls.<br />
• TAL-Blocks Program (TB) $950 + $600 Homodimer/ $700<br />
Heterodimer backbone vectors (one-time fee) <strong>Link</strong><br />
• Value Program Homodimer (XTN-V) $3,000 <strong>Link</strong><br />
• Value Program Heterodimer (H-XTN-V) $3,500 <strong>Link</strong><br />
• Guaranteed Success Program Homodimer (XTN-G) $4,000<br />
• Guaranteed Success Program Heterodimer (H-XTN-G) $4,500<br />
• Volume <strong>Pricing</strong> Available<br />
• Value & Guaranteed Success Program Deliverables: Sequence verified, transfection ready<br />
plasmid XTN TALEN.<br />
• TAL-Blocks Deliverables: Custom TAL and backbone DNA<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
Off-the-shelf XTN TALENs Click <strong>Link</strong><br />
• 80+ pre-validated and published XTNs<br />
• Targeting human genes involved in cancer or<br />
epigenetic regulation<br />
• $500/pair – sequence verified, prepped and<br />
transfection ready plasmid DNA<br />
Featured off-the-shelf XTNs<br />
-TP53 -PTEN -FOXO1 -APC<br />
-SMAD2 -KRAS -BRCA1<br />
-RARA -JAK2 -BRCA2<br />
Reyon et al. (2012) Nature Biotech 30,5.<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
CRISPR/Cas <strong>Programs</strong> & <strong>Pricing</strong> <strong>Link</strong><br />
All programs include:<br />
• Free Feasibility Study – non-binding <strong>Link</strong><br />
• Custom design- submit either the DNA sequence or gene symbol and<br />
species<br />
• Human & GFP reporter verification system controls.<br />
• CRISPR/Cas Kit (CAS-K) $300 <strong>Link</strong><br />
• CRISPR/Cas Guaranteed Success (CAS-G) $1,500 <strong>Link</strong><br />
• Volume <strong>Pricing</strong> Available<br />
• U6 promoter for cell based manipulations<br />
• T7 promoter for RNA embryo injection, KO/KI production<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
Custom Site-Specific Nuclease Reporter Vector<br />
• Activity assays apply to XTN TALEN, CRISPR/Cas & Gene Editing<br />
Projects.<br />
• Fluorescent-based reporter vector specific for your target sequence<br />
• Can be co-transfected with your site-specific nuclease to validate<br />
activity or select for cells expressing active nuclease<br />
Custom Reporter Vector Service:<br />
$500/Nuclease<br />
Custom Reporter Vector + Transfection &<br />
Activity Assay Service: $1,000 Nuclease<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.<br />
Positive results:<br />
Indicator alone: Red + Green -<br />
(% red cells in well indicates transfection efficiency)<br />
Indicator plus XTNs: Red + Green +<br />
Note: All cells may not be red and green; A positive<br />
result is many dual positive cell
Gene Editing Vector <strong>Programs</strong> & <strong>Pricing</strong> <strong>Link</strong><br />
• Genome Engineering Program – includes custom nuclease (TALEN or<br />
CRISPR/Cas) & custom donor vector (non-removable). Program is designed<br />
for knock-in and gene editing without selection & removal<br />
• Footprint-Free Gene Editing Program – includes custom nuclease (TALEN or<br />
CRISPR/Cas), Custom Footprint-Free Gene Editing Vector (with piggyBac)<br />
removable capability & Excision-only (PBx) piggyBac transposase. Program<br />
is designed for gene editing projects with selection and removal capability.<br />
• Off-the-shelf Footprint-Free Gene Editing Vectors – Vectors contain<br />
piggyBac removable capability and a variety of selection options<br />
$1,500/each <strong>Link</strong><br />
• XTN TALEN Genome Engineering Program: $5,000<br />
• Crispr/Cas Genome Engineering Program: $3,500<br />
• XTN TALEN Footprint-Free Gene Editing: $8,500<br />
• Crispr/Cas Footprint-Free Gene Editing Program: $7,000<br />
• Volume <strong>Pricing</strong> Available Order <strong>Link</strong><br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
AAVS1 – TALEN & Crispr/Cas Safe Harbor Kits<br />
AAVS-1 CRISPR AAVS-1 TALEN<br />
RFP RFP<br />
GFP GFP<br />
• Validated XTN TALEN & CRISP for AAVS1 Safe Harbor<br />
• Customizable donor vector & control donor vector<br />
• TALEN & CRISPR Safe Harbor Kit $1,500<br />
• Rosa26 Safe Harbor Kits – coming soon<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
TALEN or CRISPR/Cas vs. KOMP ES Cell Clone<br />
• $ 1,470.00 Targeted ES cells, First Clone, Fully Verified*<br />
• *30% of all KOMP clones fail genetic analysis<br />
• KOMP recommends 3 clones per targeted allele for 3 different injections<br />
• Real KOMP Price = $3,100 (Clones) + 3 X $ 6,323.00 = $18,969 Average Grant total<br />
= $22,069<br />
• <strong>Transposagen</strong> XTN TALENs or CRISPR/Cas ($5,000) + Injection (1) $ 6,323 =<br />
Average Grant Total ~$ 11,323<br />
KO Mouse Price<br />
25000<br />
20000<br />
15000<br />
10000<br />
5000<br />
0<br />
KOMP <strong>Transposagen</strong><br />
• Knockout Mice From <strong>Transposagen</strong> 50% less expensive than KOMP<br />
• The researcher choses the target - you are in control<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
High Efficiency Cell Line Engineering Service<br />
Knockout Knock-In Stable Expression<br />
Gene Editing: Single Base-pair changes<br />
Stem cells, CHO, HEK293, 3T3, other cell types available<br />
Milestone Based Payments<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
Custom Rat & Mouse Model Creation Services<br />
* Indicates<br />
potential<br />
founders<br />
Consistently achieve 20% founder efficiency<br />
Outbred & inbred lines available<br />
Milestone 1: vector construction<br />
Milestone 2: pronuclear injection & screening<br />
Milestone 3: germline transmission & delivery<br />
Request Quote <strong>Link</strong><br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
Thank You!<br />
© <strong>Transposagen</strong> Biopharmaceuticals, Inc.
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