Alice Rotini - FedOA - Università degli Studi di Napoli Federico II

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RAPD genetic analysis RESULTS The overall percentage of polymorphism in the S. Marinella meadow, obtained on 2005 samples through the calculation of amplified polymorphic and monomorphic fragments, was 63.41%. All the 10 primers gave high reproducibility of their electrophoresis pattern both in the signal intensity and in the number of bands for each different analysis performed. Cluster analysis (fig. 3.6) confirmed that similarity between samples is very high (the highest genetic distance being 0.82). The average of all similarity coefficients among the samples was 0.87±0.03. The Mantel test comparing Nei's distance and cophenetic matrices had a strong and statistically significant correlation with a value of r = 0.9 as a measure goodness of fit for cluster analysis. Fig. 3.6. UPGMA phenogram constructed from the matrix of RAPD-based genetic distances among P. oceanica samples at S. Marinella. Antioxidant enzyme activity and photosynthetic pigments A protocol for the activity determination of two enzymes involved in antioxidant defence (DHAR, dehydroascorbate reductese, and APx, ascorbate peroxidase) was developed for the first time on P. oceanica leaves. Preliminary data on S. Marinella and Monterosso 2010 samples are reported in table 3.4 and fig. 3.7. 35
36 Antioxidant enzyme activity and photosynthetic pigments Table 3.4. Dehydroascorbate reductase (DHAR) and ascorbate peroxidase (APx) activities in P. oceanica leaves, sampled in 2010 from S. Marinella (SM) and Monterosso (M) meadow. Mean concentration ± SE (standard error) from three replicates. Sample DHAR APx (µmole of ascorbate formed/mg protein/min) (µmole of ascorbate oxidized/mg protein/min) SM 1 0.176 ± 0.003 0.547 ± 0.015 SM 2 0.283 ± 0.001 0.495 ± 0.037 SM 3 0.437 ± 0.009 0.434 ± 0.170 SM 4 0.220 ± 0.004 0.828 ± 0.037 SM 5 0.101 ± 0.000 0.504 ± 0.001 SM 6 0.498 ± 0.016 1.289 ± 0.431 SM 7 0.440 ± 0.007 1.085 ± 0.005 SM 8 0.250 ± 0.002 0.860 ± 0.008 SM 9 0.331 ± 0.002 0.875 ± 0.016 SM 10 0.324 ± 0.002 0.731 ± 0.014 M 1 0.164 ± 0.003 0.353 ± 0.017 M 2 0.157 ± 0.002 0.523 ± 0.006 M 3 0.143 ± 0.002 0.356 ± 0.017 M 4 0.177 ± 0.011 0.670 ± 0.004 M 5 0.167 ± 0.002 0.483 ± 0.004 M 6 0.144 ± 0.004 0.517 ± 0.008 M 7 0.188 ± 0.012 0.622 ± 0.008 M 8 0.206 ± 0.008 0.942 ± 0.016 M 9 0.178 ± 0.003 0.566 ± 0.009 M 10 0.270 ± 0.021 0.479 ± 0.005 M 11 0.143 ± 0.005 0.386 ± 0.002 M 12 0.256 ± 0.008 0.477 ± 0.010 A good correlation (r=-0.8; p
Page 1 and 2: PhD Thesis Alice Rotini
Page 3 and 4: Università degli studi di Napoli
Page 5 and 6: To Lina, my grandmother
Page 7 and 8: Acknowledgments Completing a thesis
Page 9 and 10: 8 Posidonia oceanica (L.) Delile 1.
Page 11 and 12: 10 Seagrass meadows: ecological rol
Page 13 and 14: 12 Seagrass meadows: ecological rol
Page 15 and 16: 14 P. oceanica monitoring: traditio
Page 17 and 18: 16 P. oceanica monitoring: traditio
Page 19 and 20: 18 Study Site and Sampling 2. MATER
Page 21 and 22: 20 Study Site and Sampling The Mont
Page 23 and 24: 22 Total phenols Z. noltii samples
Page 25 and 26: 24 Heavy metal/element content Elec
Page 27 and 28: 26 Antioxidant enzyme activity, sol
Page 29 and 30: 28 Antioxidant enzyme activity, sol
Page 31 and 32: 30 Total phenols content among the
Page 33 and 34: 32 2-D electrophoresis analysis 2-D
Page 35: 34 Heavy metal/element content Fig.
Page 39 and 40: 38 Antioxidant enzyme activity and
Page 41 and 42: 40 Total phenols ranging from very
Page 43 and 44: 42 2-D electrophoresis analysis sli
Page 45 and 46: 44 Heavy metal/element content Heav
Page 52 and 53: 6. REFERENCES REFERENCES Andrade C.
Page 54 and 55: REFERENCES coastal waters of Valenc
Page 56 and 57: REFERENCES Mittler R., Puolus T. L.
Page 58 and 59: REFERENCES Sanchez-Jerez P., Ramos-

Alice Rotini - FedOA - Università degli Studi di Napoli Federico II

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