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Abstracts 2011 - Framingham State University

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Jaqueline Taravella<br />

Larval Substrate Preference by the<br />

Invasive Tunicate Botrylloides<br />

violaceus.<br />

Ascidians are a group of filter feeding marine invertebrates that are successful invasive<br />

species. These invasive species have negative impacts on the fishing industry<br />

because they out compete native shellfish, such as clams and mussels, for space. In<br />

addition they increase maintenance cost for fishermen because they often grow on<br />

nets and other equipment. Botrylloides violaceus is one species, introduced from<br />

eastern Asia, which is considered invasive in the Gulf of Maine. B. violaceus<br />

reproduces June to September by brooding their larvae; larvae are released from the<br />

adult and then settle onto a substrate within a few hours. Two surveys were done<br />

before and after reproductive season at three intertidal sites in the Gulf of Maine to<br />

determine percent cover of potential substrates present and the number of B.<br />

violaceus colonies present. Choice experiments were conducted with larvae from<br />

colonies scraped off of docks. Larvae were placed in containers with choice of two<br />

substrates. A previous study showed B. violaceus most commonly on Fucus<br />

vesiculosus so larvae were given a choice of F. vesiculosus versus the other<br />

substrates identified during the intertidal survey (Ascophyllum nodosum, Chondrus<br />

crispus, and rock). The results of the substrate preference experiments showed that B.<br />

violaceus preferred rock and A. nodosum over F. vesiculosus. These results are not<br />

consistent with the previous study since B. violaceus was found more commonly on F.<br />

vesiculosus than other substrates present.<br />

Botrylloides violaceus<br />

• Irregular flat sheet,<br />

colonial tunicate<br />

• Brood larvae<br />

• Common exhalent<br />

siphon<br />

• Colors: orange to red to<br />

purple<br />

http://www.cabi.org/isc/?compid=5&dsid=107828&loadmodule=datasheet&page=481&site=144<br />

Results: Substrate Preference<br />

Substrate Pairing Chi-Squared Value<br />

Fucus vesiculosus < Ascophyllum nodosum 6.65*<br />

Fucus vesiculosus > Chondrus crispus 2.06<br />

Fucus vesiculosus < Rock 12.35*<br />

Fucus vesiculosus < Nothing 0.31<br />

Nothing > Nothing 0.54<br />

Glue > Nothing 3.59<br />

(* Indicates p-value of less than 0.05)

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