End-point vs.Real-time PCR & RT-PCR Workflow - Gibthai

End-point vs. Real-time
PCR & RT-PCR Workflow
August 1 - September 30, 2012

End-point vs. Real-time
PCR & RT-PCR Workflow
August 1 - September 30, 2012
End-point PCR & RT-PCR Workflow
Real-time PCR & RT-PCR Workflow
Selection Guide: One-Step vs. Two-Step Kits
Choose the 1-Step Kit, if you...
• Do not store cDNA
• Dispose samples after one or few uses
• Have many samples with one or few targets
• Use liquid handling robotics
• Choose to reduce chance of cross
contamination during procedure
• Need to reduce time to results
Choose the 2-Step Kit, if you...
• Need to store cDNA
• Have limited sample quantity
• Have many targets per sample
• Require maximum performance of both
RT and PCR steps

End-point vs. Real-time
PCR & RT-PCR Workflow
August 1 - September 30, 2012
DNA/RNA Extraction
Cat.No. Description Size Price List Promo Price
1IVL11-K1820-01 PURELINK GENOMIC DNA MINI KIT 50 RXNS 6,500.00 5,200.00
1IVL11-K1820-02 PURELINK GENOMIC DNA MINI KIT 250 RXNS 28,000.00 23,000.00
1IVL11-K1830-01 PURELINK GENOMIC PLANT DNA 50 RXNS 8,000.00 6,500.00
1IV11-12280-050 PURELINK VIRAL RNA/DNA KIT 50 RXNS 9,000.00 6,500.00
1IV11-12183-018A PURELINK RNA MINI PURIFICATION KIT 50 RXNS 12,000.00 9,500.00
1IV11-K1560-01 PURELINK TOTAL RNA BLOOD KIT 50 RXNS 13,500.00 11,000.00
1IV11-10503-027 DNAZOL Reagent 100 ml 8,500.00 6,300.00
1IV05-25530-015 PROTENASE K 100 ml 6,200.00 2,800.00
1IV11-15596-026 TRIZOL Reagent 100 ml 7,500.00 5,000.00
1IV11-10296-010 TRIZOL LS Reagent 100 ml 11,000.00 7,000.00
1ABN-7020 RNAlater® RNA Stabilization Solution 100 ml 8,120.00 4,800.00
1ABN-7024 RNAlater® RNA Stabilization Solution 250 ml 17,620.00 10,500.00
Quantitative
Qubit® 2.0 Fluorometer
For your precious DNA/RNA samples
The new features include:
–– Large LCD color touch screen
–– Automatic data logging and USB port for data management
–– More Accurate Results than with UV Absorbance
–– Standard curve display after calibration completion
–– Small sample volume requirement (1–20 µl)
Qubit® 2.0 MEGA SALE ‡æ’¬ß 69,000 ∫“∑‡∑Ë“π—Èπ
Ordering Information
Cat.No. Description Size Price List Promo Price
1IVM-Q32866 Qubit® 2.0 Fluorometer 1 EA 140,000.00 69,000.00
1IVM-Q32850 QUBIT dsDNA BR ASSAY KIT 1 KIT 5,550.00 3,000.00
1IVM-Q32851 QUBIT dsDNA HS ASSAY KIT 1 KIT 5,550.00 3,000.00
1IVM-Q32852 QUBIT RNA ASSAY KIT 1 KIT 5,550.00 3,000.00
1IVM-Q33211 QUBIT PROTEIN ASSAY KIT 1 KIT 5,550.00 3,000.00
(¢Õß«π‘∑∏‘ω¡Ë√Ë«¡√“¬°“√ Special Gift & MEGA Gift

End-point vs. Real-time
PCR & RT-PCR Workflow
August 1 - September 30, 2012
End-point PCR& RT-PCR ENZYME& KIT
Cat.No. Description Size Price List MEGA OFFER
1IV05-11615-010 Taq DNA Polymerase 500 U 2,000.00 Buy 10 Get 1 Free!
Cat.No. Description Size Price List Promo Price
1IV05-10966-030 Platinum Taq DNA Polymerase 500 U 6,000.00 4,000.00
1IV05-11304-011 Platinum Taq High Fidelity 100 RXNS 10,000.00 6,300.00
1IV05-10480-010 Elongase Enzyme Mix 100 RXNS 12,000.00 6,700.00
1IV02-11708-013 PLATINUM PFX DNA POLYMERASE 100 RXNS 12,500.00 5,300.00
1IV02-12346-086 AccuPrime Taq DNA Polymerase 200 rxns 19,800.00 14,000.00
1IV02-10572-014 PCR SUPER MIX 100 rxns 6,700.00 4,900.00
1IV02-18427-013 10 mM dNTP Mix 100 ul 4,200.00 1,800.00
1IV02-10297-018 100 mM dNTP Set 4x25 umol 15,000.00 9,600.00
Cat.No. Description Size Price List Promo Price
1IV02-18080-044 Superscript TM III Reverse Transcriptase 10000 U 13,000.00 9,000.00
1IV02-12574-026 Superscript TM III One- Step with Platinum Taq 100 RXNS 28,000.00 18,000.00
1IV02-18080-051 Superscript TM III First-Strand Synthesis system 50 RXNS 23,000.00 16,500.00
Real-time PCR & RT-PCR KIT
Two-step Kits
Cat.No. Description Size Promo Price 1-3 Kits MEGA Price 4 Kits UP
1IV01-11754-050 Superscript® VILO TM cDNA Synthesis Kit 50 RXNS 15,000.00 12,500.00
Cat.No. Description Size Price List Promo Price
1IV01-11762-100 SYBR GreenER TM qPCR SuperMix Universal 100 rxns 15,000.00 7,700.00
1IV01-11733-038 Platinum® SYBR® Green qPCR SuperMix-UDG 100 rxns 17,500.00 11,000.00
1IV01-11733-046 Platinum® SYBR® Green qPCR SuperMix-UDG 500 rxns 76,000.00 38,000.00
1IV01-11730-017 Platinum® qPCR SuperMix-UDG 100 rxns 14,000.00 9,850.00
1IV01-11784-200 EXPRESS SYBR® GreenER TM qPCR Supermix 200 rxns 25,000.00 15,000.00
1IV01-11785-200 EXPRESS qPCR Supermix Universal 200 rxns 28,000.00 16,500.00
One-step Kits
Cat.No. Description Size Promo Price 1-4 Kits MEGA Price 5 Kits UP
1IV01-11732-020 SuperScript TM III Platinum® 100 rxns 16,000.00 14,000.00
One-Step qRT-PCR Kit
Next-generation, SYBR® qRT-PCR reagents designed to complement fast-cycling instruments
Cat.No. Description Size Price List Promo Price
1IV01-11780-200 EXPRESS One-Step SYBR® GreenER TM Universal 500 rxns 54,250.00 34,000.00
1IV01-11781-200 EXPRESS One-Step Superscript® qRT-PCR Universal 500 rxns 54,750.00 34,000.00

End-point vs. Real-time
PCR & RT-PCR Workflow
August 1 - September 30, 2012
Electrophoresis
Cat.No. Description Size Price List Promo Price
1IV18-75510-019 AGAROSE 100 G 5,000.00 Buy 1 Get 1!
Cat.No. Description Size Price List Promo Price
1IV13-10488-058 Track-IT 100 BP DNA LADDER 100 rxns 7,400.00 4,000.00
1IV13-10488-085 Track-IT1 KB PLUS DNA LADDER 100 rxns 8,200.00 4,400.00
1IV13-10821-015 10 BP DNA LADDER 50 UG 7,400.00 3,400.00
1IV13-10597-011 25 BP DNA LADDER 50 UG 7,400.00 3,700.00
1IV13-10416-014 50 BP DNA LADDER 50 UG 7,400.00 3,700.00
1IV13-15628-019 100 BP DNA LADDER 75 UG 6,500.00 3,800.00
1IV13-15613-011 123 BP DNA LADDER 100 UG 7,400.00 3,250.00
1IV13-10787-018 1KB PLUS DNA LADDER 250 UG 9,400.00 4,950.00
Visualization
Cat.No. Description Size Price List Promo Price
1IVM-S33102 SYBR Safe DNA Gel Stain 400 UL 3,600.00 2,500.00
1IVM-S11494 SYBR Gold Nucleic Acid Gel Stain 500 UL 8,700.00 5,800.00
1IVM-S7563 SYBR® Green I Nucleic Acid Gel Stain - 10,000X 500 UL 18,220.00 8,000.00
concentrate in DMSO
1IV13-10816-015 10X BLUEJUICE LOADING BUFFER 3x1 ml 2,770.00 1,400.00
DNA Purification
Cat.No. Description Size Price List Promo Price
1IV11-K3100-01 Purelink PCR Purification Kit 50 rxns 3,700.00 2,900.00
1IV11-K2100-12 PureLink Quick Gel Extraction Kit 50 rxns 4,000.00 2,700.00
Related Products
Cat.No. Description Size Price List Promo Price
1IV18-10977-015 DNASE/RNASE FREE DISTILLED WATER 500 ML 1,400.00 1,100.00
1ABN-AM9915G Ambion DEPC-Treated Water 100 ml 2,080.00 1,200.00
1ABN-AM7005 Ambion RNAsecure TM Reagent 1 ml 2,375.00 1,400.00
1ABN-AM7006 Ambion RNAsecure TM Reagent 10 ml 11,480.00 6,800.00
1IV02-10777-019 RNASEOUT RECOMBINATION RNASE INHIBITOR 5000 U 8,200.00 6,100.00
1GSC-10-228 RNAse Free, 475 ml 475 ml 1,800.00 1,100.00
1GSC-10-173 RNAse Free, 1 L 1 L 3,300.00 1,980.00
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Special Gift
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√—∫ø√’ Flipper Rack for 0.5/1.5mL
Tube (#1GB-KJ809) ®”π«π 1 Õ—π
Gift
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August 1- September 30, 2012
Super HELIX Promotion
Reliable Partner for DNA/RNA
Amplification
HelixCript TM Thermo Reverse Transcriptase
Cat.No. Description Size Price List Promo Price
1NNH-RT10K HelixCript Thermo Reverse Enzyme 10000 u 8,000.00 4,000.00
1NNH-RT10KN HelixCript Thermo Reverse Enzyme (with dNTPs) 10000 u 8,000.00 4,000.00
HelixCript TM Conventional RT-PCR Kits
Cat.No. Description Size Price List Promo Price
1NNH-ORTH100 HelixCript one-step RT-PCR kit (Hypersense) 100 rxns 15,000.00 7,500.00
1NNH-TRTH100 HelixCript Two-step RT-PCR kit (Hypersense) 100 rxns 20,000.00 10,000.00
HelixAmp TM Taq DNA Polymerase
Cat.No. Description Size Price List Promo Price
1NNH-TBF500 HelixAmp Taq 500 u 1,800.00 900.00
1NNH-TBF500N HelixAmp Taq (with dNTPs) 500 u 3,000.00 1,650.00
HelixAmp TM Hot-Start Taq DNA Polymerase, Chemically-Modified
Cat.No. Description Size Price List Promo Price
1NNH-HT250N HelixAmp Hot Taq (with dNTPs) 250 u 4,500.00 2,250.00
1NNH-HT5000 HelixAmp Hot Taq 5000 u 31,500.00 17,400.00
Note: These prices are VAT 7% excluded
Special Gift
´◊ÈÕ‘π§È“‚ª√‚¡—Ëππ’ȧ√∫ 12,000 ∫“∑
√—∫ø√’ Flipper Rack for 0.5/1.5mL
Tube (#1GB-KJ809) ®”π«π 1 Õ—π
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