Surface-Enhanced Raman Scattering (SERS) - iaria

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SERS vs fluorescence spectroscopy • Fluorescence is very efficient – SMD • Fluorescence is currently a well-established technique • SERS has high specificity, providing a unique ‘fingerprint’ – background distinguish, multiplexing • SERS is applied directly to the molecule, no fluorophore • Any excitation wavelength for SERS • Higher spectral specicity • Infrared excitation August 19, 2012 ICQNM 2012 Rome, Italy 74
Biological applications of SERS • Intracellular measurements • SERS images (optical bioimagin) • SERS labels for biomolecule identifying • Biocompatible nanosensors • Glucose measurement in vivo • Characterization of bacteria • DNA detection August 19, 2012 ICQNM 2012 Rome, Italy 75
Page 1 and 2:
Surface-Enhanced Raman Scattering (
Page 3 and 4:
Pros and cons of Raman (SERS) • A
Page 5 and 6:
Energy levels =2 Excited electron s
Page 7 and 8:
Dipole moment in a molecule 0 - mo
Page 9 and 10:
IR vs Raman Polystyrene Senses dipo
Page 11 and 12:
Stokes vs anti-Stokes Rayleigh line
Page 13 and 14:
Cross-sections of the optical proce
Page 15 and 16:
Steps of Raman development • Lase
Page 17 and 18:
SERS experiment with pyridine adsor
Page 19 and 20:
First experience • SERS condition
Page 21 and 22:
Deilectric function of metals E.C.
Page 23 and 24: Optical properties of metals Re() i
Page 25 and 26: Plasmonic welding Before illuminati
Page 27 and 28: Electric field outside of metal sph
Page 29 and 30: Distance dependence Pyridine, Ag ov
Page 31 and 32: Wavelength dependence benzenethiol,
Page 33 and 34: SERRS • The energy of the incomin
Page 35 and 36: Chemical enhancement EF EM , EF CE
Page 37 and 38: SERS enhancement factor Analytical
Page 39 and 40: Inetrmediate conclusion II • High
Page 41 and 42: Metal colloids for SERS • Mainly
Page 43 and 44: SPR of colloids Observed effects ar
Page 45 and 46: Gold Lace Nanoshells PU means amphi
Page 47 and 48: Nanoshells 4-mercaptobenzoic acid (
Page 49 and 50: ’Planar’ substrates - metal isl
Page 51 and 52: ’Planar’ substrates - nanoengin
Page 53 and 54: Nanohole array 100 nm thick Au 200
Page 55 and 56: Pillar based substrate SiO2 d=150nm
Page 57 and 58: Commersial SERS substrate (Klarite)
Page 59 and 60: Single-molecule detection • Compe
Page 61 and 62: SERS fluctuation sources • Photo-
Page 63 and 64: Raman instrumetation Excitation and
Page 65 and 66: Portable Raman Stage Optical fiber
Page 67 and 68: Single walled carbon nanotube on Si
Page 69 and 70: Fabrication method of metal nanostr
Page 71 and 72: Large and small particles on the sa
Page 73: EF measurement thioglycerol Used fo
Page 77 and 78: Summary • Metal nanostructures pr
Page 79 and 80: Questions • Is it possible to do
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Surface-Enhanced Raman Scattering (SERS) - iaria

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