Nuclear Transplantation in Amoebae. I. - Journal of Cell Science

Lorch and Danielli—Nuclear Transplantation in Amoebae. I 459
TABLE 3. Amoeba discoides. Nuclear diameters of rapidly growing cultures.
The standard deviations of the average nuclear diameters are approximately 3-5.
Serial
number
Di
D$a
V>sb
Dio
Du
D12
Dig
D2fl
D8a
D13
D. 4
D17
D18
Approx.
Number
of
amoebae
present
80
80
200
200
120
120
100
60
70
IOO
IOO
80
80
Age of
clone
(days)
16
IS
20
#
12
H
16
16
18
18
Date
examined
IS/I2/S°
1/3/51
6/3/51
28/4/51
28/4/51
28/4/51
22/11/51
1/2/51
10/4/51
26/4/51
26/4/51
28/4/51
28/4/51
Number
measured
47
50
100
5°
50
50
5°
47
5°
50
5°
5°
50
Average
nuclear
diam. p
35
40
39
37
37
37
40
36
34
39
39
39
42
Nuclear
diam. of
parent
of clone
33
• • )
•• 1
•• 1
3°
36
30
42
Comments
same culture. Cf. Dsc,
table 4
*Dio-i2 and D19 kept
under 'equalized' conditions.
See text. Cf. Pi 3-
15 and P19
clones derived from homotransfers.
Cf. table 4
large stellate amoebae probably
no divisions for 2
days
TABLE 4. Amoeba discoides. Nuclear diameters of mass cultures (clones) of over
1,000 amoebae in different conditions. The standard deviations of the average
nuclear diameters are approximately 3-5.
Serial
number
D Sc
D15
D16
Dzb
Vzc
DSb
State of culture
starving, sluggish
small stellate amoebae,
flagellate food only .
very active, well fed .
Date
examined
27/3/51
8/3/51
13/3/51
23/3/51
29/3/51
23/4/51
Number
measured
50
100
IOO
50
50
50
Average
nuclear
diam. /i
38
36
40
39
36
38
Comments
cf. Dsa and b (same culture)
table 3
derived from homotransfers.
same culture as D2a
(table 3) 8 weeks later.
same culture as D8a (table
3) 2 weeks later
Hh