Leaf has 3 axes:1) proximodistal, 2) centrolateral, 3) ab-adaxial ...
Leaf has 3 axes:1) proximodistal, 2) centrolateral, 3) ab-adaxial ...
Leaf has 3 axes:1) proximodistal, 2) centrolateral, 3) ab-adaxial ...
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Phantastica<br />
•PHAN is a MYBtranscription<br />
factor<br />
•Expressed uniformly<br />
throughout the leaf<br />
primordia<br />
•May respond to<br />
putative <strong>adaxial</strong>izing<br />
signal from the SAM<br />
•PHAN is orthologue to<br />
AS1 in Ar<strong>ab</strong>idopsis and<br />
ROUGH SHEATH2<br />
(RS2) in maize<br />
•KNAT1 and KNAT2<br />
overexpressed in AS1<br />
Bowman et al., 2002 leaves<br />
wt<br />
Filamentous flowers (fil)<br />
have flowers missing<br />
petals and stamens (B) or<br />
the only form filaments<br />
FIL = zinc finger<br />
transcription factor<br />
Sawa et al. 1999<br />
X-Gluc<br />
DAP<br />
I<br />
Wt - trichomes on <strong>adaxial</strong><br />
35S::FIL - filaments, no trichomes<br />
35S::GUS<br />
35S::FIL::<br />
GUS<br />
Wild type surface<br />
FIL localizes to the nucleus - transcription factor<br />
Member of gene family, YABBY3, also causes <strong>ab</strong>axialization<br />
35S::FIL surface<br />
Sawa et al. 1999<br />
Overexpression of FIL (35S::FIL) leads to <strong>ab</strong>axialized filamentous leaves<br />
(no trichomes) or leaves with patches of <strong>ab</strong>axialized cells amongst <strong>adaxial</strong><br />
3
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