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Microscopy
Core Facility
Andreas Vonderheit
Education
2000 Diploma in Molecular Biology, ZMBH, University of Heidelberg
2004 PhD in Biochemistry, ETH, Zürich
Positions held
2005-2008 Postdoctoral Researcher, Institute of Nanotechnology, ETH, Zürich
2008-2011 Screening Scientist at the RNAi Image-based Screening Center (RISC)
at the Light Microscopy Centre (LMC), ETH, Zürich
Since 2011 Head of Microscopy, Core Facilities and Technology,
Institute of Molecular Biology (IMB), Mainz
Core Facility Overview
The Microscopy Core Facility not only provides hands-on training and
access to various state-of-the-art microscopes, but also offers further
education through the provision of a variety of practical courses and
lectures. Training lectures provided range from a general “Introduction
to Microscopy” to “Pitfalls in Image Acquisition” and “Ethics in Image
Acquisition and Processing”. Lectures, courses and the scientific services
available are all open to Mainz’ scientific community. To date the lectures
have attracted a large number of non-IMB researchers, with about
80% of the audience coming from Johannes Gutenberg University
and the University Medical Center. The microscopes provided are
utilized equally by IMB staff and external users.
Services Offered
The facility provides hands-on training and access to various ultramodern
confocal laser scanning microscopes, super-resolution microscopes
and motorized fast-flourescence microscopes for live-cell
microscopy. There are also PCs for image processing equipped with
software for deconvolution and 3D-rendering. The following microscopic
equipment, a large part of which was financed by DFG, is available:
• M80 Demonstration Stereo Microscope: Equipped with a camera
and a 24“ monitor for teaching
• M205 FA Fluorescence Stereo Microscope: Equipped with a
camera, fluorescence light source and three filters for UV, green,
and red fluorophores
• DM2500 Fluorescence Upright Microscope: Equipped with a
colour camera, fluorescence light source and three filters for UV,
green, and red fluorophores and with five air objectives (this microscope
is perfectly suited for histology)
• AF7000 Widefield Fluorescence Microscope: This widefield microscope
is equipped with an incubator box for live-microscopy, fast
filter wheels and a fast camera
• TCS LSI Macro Zoom Confocal: This microscope combines a stereo
microscope with a confocal. Organisms like C. elegans or Drosophila
larvae can be scanned as a whole or zoomed down to single
cell level in confocal mode
• TCS SPE Confocal Microscope: An upright confocal microscope
with one detector
• TCS SP5 Confocal Microscope: An inverse microscope with four
PMTs, four lasers, and a fast resonance scanner
• TCS STED CW Super-Resolution Microscope: A super-resolution
microscope which allows live microscopy and FCS. Equipped with
an incubation box, two normal PMTs and two HyD detectors
• SR GSD Super-Resolution Microscope (localization method):
A super-resolution microscope on which TIRF is possible
• Image Processing Station
Figure 1. Mitochondria network in CHO cells labeled with Mitotracker® and imaged with
a confocal microscope. (A) One plane of a z-stack. (B) Same plane after deconvolution. (C)
Cutout of A. (D) Cutout of B. Scalebar: 4 µm. Imaged by A. Vonderheit.