Confirmed Nursery Protocol - aphis

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TABLE OF CONTENTS INTENDED USE _____________________________________________________________ 3 GOAL ______________________________________________________________________ 4 DISCLAIMERS ______________________________________________________________ 4 Definitions __________________________________________________________________ 5 Trigger Events for Use of Protocol ________________________________________________ 9 Authorities __________________________________________________________________ 9 Communicate and Notify ______________________________________________________ 10 Conduct Investigations ________________________________________________________ 11 Secure the Nursery ___________________________________________________________ 12 Survey the Nursery and Perimeter _______________________________________________ 13 Disinfest the Nursery _________________________________________________________ 15 Ninety (90) Day Quarantine Activities ____________________________________________ 17 Release the Nursery __________________________________________________________ 19 Post eradication monitoring ____________________________________________________ 19 APPENDICIES Appendix 1 APHIS List of Regulated Hosts and Plants Associated with Phytophthora ramorum ____ 20 Appendix 2 Schematic of a Nursery with Infected Plant(s) _______________________________ 21 Appendix 3 Contacts __________________________________________________________ 22 Appendix 4 Delimiting Survey Protocol ___________________________________________ 23 Appendix 5 Diagnostics _______________________________________________________ 25 Appendix 6 Soil and Container Mix Protocol _______________________________________ 26 Appendix 7 Water Sampling Protocol ____________________________________________ 38 Appendix 8 List of Treatment and Disinfection options ______________________________ 50 Appendix 9 Biosecurity Measures for Nurseries ____________________________________ 55 Appendix 10 Reserved ________________________________________________________ 59 Appendix 11 Mitigations for Retail Nurseries Found with P. ramorum More Than Once ____ 60 Confirmed Nursery Protocol: Version 8.1 Revised: July 3, 2013 2
INTENDED USE The intended use of this Regulatory Protocol is for nurseries containing plants infected with Phytophthora ramorum; the nursery types are: 1) Interstate shipping retail nurseries and brokers, and, 2) Intrastate and interstate shipping wholesale and production nurseries. This protocol refers to wholesale and productions nurseries throughout the document; include interstate shipping retail nurseries and brokers when wholesale or production nurseries are mentioned. In February 2005, USDA Animal and Plant Health Inspection Service (APHIS) Plant Protection and Quarantine (PPQ) published an interim rule revising federal domestic regulations for Phytophthora ramorum (7 CFR 301.92). The complete text and other information may be found at the USDA APHIS PPQ web site: http://www.aphis.usda.gov/plant_health/plant_pest_info/pram/ Since the regulations were first published in 2002, P. ramorum has been detected in a significant number of nurseries. These detections prompted the need for a standard protocol for use by state and federal regulators to respond to finds of P. ramorum in nurseries. To ensure that there is consistency in responding to infestations of P. ramorum, this protocol describes the official activities performed within and around nurseries by USDA APHIS staff in cooperation with state agriculture regulatory officials. Nurseries found with P. ramorum infestations more than once P. ramorum infestations in nurseries may also be re-introduced by the above means, or the effort to eradicate the disease may fail. In the event that a nursery has P. ramorum detected on site after the initial release from the Emergency Action Notification (EAN) or state equivalent, it is necessary to implement additional measures to ensure that the risks associated with P. ramorum are properly mitigated. See Appendix 11 for details of these additional measures. Confirmed Nursery Protocol: Version 8.1 Revised: July 3, 2013 3
Page 1: Official Regulatory Protocol for Nu
Page 5 and 6: Definitions Associated plants: Bios
Page 7 and 8: the necessity for Federal confirmat
Page 9 and 10: Trigger Events for Use of Protocol
Page 11 and 12: Conduct Investigations Trace Forwar
Page 13 and 14: Survey the Nursery and Perimeter Th
Page 15 and 16: The purpose of the perimeter survey
Page 17 and 18: Ninety (90) Day Quarantine Activiti
Page 19 and 20: Release the Nursery Nurseries and t
Page 21 and 22: Appendix 2 Schematic of Nursery wit
Page 23 and 24: Appendix 4 Delimiting Survey Protoc
Page 25 and 26: Appendix 5 Diagnostics Revised: Apr
Page 27 and 28: Sampling Soils and Container Mixes
Page 29 and 30: 7. Disinfest sampling tools and sol
Page 31 and 32: and curl up. There should be six pl
Page 33 and 34: Second Baiting of Soil and/or Conta
Page 35 and 36: Growing Media Formulae PARPH-V8 Sel
Page 37 and 38: References Jeffers, S.N., and Marti
Page 39 and 40: leaves. Present year leaf growth ma
Page 41 and 42: • Place all sample bags in an ins
Page 43 and 44: surface of the funnel with distille
Page 45 and 46: Figure I-3. Completed bait station
Page 47 and 48: Sample Processing • Bottles conta
Page 49 and 50: Pages 55-58 in: Proceedings of the
Page 51 and 52: quarantine block. The following tab
Page 53 and 54:
Fumigant Trade names Comments Vapam
Page 55 and 56:
Appendix 9 Biosecurity Measures for
Page 57 and 58:
Boots: Rubber boots which can be di
Page 59 and 60:
Appendix 10 Confirmed Nursery Proto
Page 61 and 62:
3. 45 days after implementation of
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Confirmed Nursery Protocol - aphis

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