Issue 1 - Thermo Fisher

Take Your Cell-based Assays to the Edge
Application Note
In cell culture, a volume loss as
small as 10% concentrates media
components and metabolites
enough to alter cell physiology, in
some cases, severely. With this in
mind, Thermo Scientific recently
unveiled its latest advancement in
plates for cell culture.
The Nunc Edge 96-well plates for
cell-based assays incorporate large
perimeter evaporative buffer zones
that eliminate well-to-well variability,
while dramatically reducing the
overall plate evaporation rate to
lower that 2% after seven days
of incubation.
And what’s the result? Tests have
shown more viable and healthy
cell yields are obtained.
The unique buffer zones enable
the use of all 96 wells on the plate
and greatly reduce the edge effect
commonly experienced in cell culture
and maintaining data consistency
throughout the plate.
The implications of volume loss can
concentrate media components
and metabolites enough to alter cell
physiology, a phenomena which is
more pronounced in the outer and
corner wells. The Nunc Edge plates
significantly reduce this occurrence,
effectively maintaining sample
concentrations over long periods of
incubation. The prevention of cell
death and toxicity in the plates’ outer
wells allows results to remain more
true to the population phenotype
for more efficient, high throughput
analysis.
In cell-based assays where multiple
targets are simultaneously imaged,
all the fluorescence from a single
location needs to focus at the same
point within the imaging system. The
flatness of the plates eliminates the
occurrence of chromatic aberrations,
enabling efficient automated
imaging. Furthermore, varying
reagent concentrations from assay
washing/aspiration steps are also
dramatically reduced.
Advantages of Thermo Scientific
Nunc Edge Plate
• Low-evaporation “moat”
• 96 well format - follows ANSI standard
footprint and is amenable to automation
• Simply fill moat with sterile H 2
O and
eliminate edge effects
• Cell culture treated or non-treated
• Low fluorescence
• Custom barcoding available – the safest
way to track your samples
• Designed for both automated
or manual use
• Superior imaging
For more information please contact:
Tina McLure (AU) tina.mclure@thermofisher.com
or Ph: +61 3 9757 4382
Dr Jerry Wong (NZ) jerry.wong@thermofisher.com
or Ph: +64 9 980 6768
Vita means Life : NEW Thermo Scientific
Nunc Nunclon Vita Surface
Animal component-free surface for growth of stem cells & other fastidious cells.
Nunclon Vita Surface supports
attachment, colony formation and
growth of human ESC, human IPS
cells and other fastidious cell lines in
the absence of feeder cells and matrix
coatings. In media supplemented with
ROCK inhibitor, human ESC can be
cultured on the Nunclon Vita Surface
for at least 10 passages without loss
of pluripotency. Human ESC has
successfully been expanded for more
than 10 passages without changes to
the karyotype.
The unique Nunclon
Vita surface helps
remove variability, requires
less work, and allows for the
treatment surface to be adaptable
to scalable cell expansion.
Human Embryonic Stem Cells
(human ESC)
• No matrix coating or feeder cells necessary
• Expand for more than 10 passages in conditioned
media with a ROCK inhibitor while maintaining pluripotency
• Passage with enzymes or by mechanical selection
• Alternatively, passage by withdrawing ROCK inhibitor followed by
a short incubation and gentle pipetting
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