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GC CAPILLARY & PACKED COLUMNS Internal Diameter The column internal diameter is inversely proportional to its separation power. The smaller the diameter, the larger the efficiency and thus a higher resolution but at the same time the loading capacity decreases. For samples containing a large number of substances where you may need a given resolution, it is recommended to use small internal diameter columns (0.20-0.25 mm) and for samples with a high range of concentrations higher internal diameter columns are recommended (0.32-0.53 mm) since these larger diameters allow for the injection of a higher sample amount. Columns of 0.53 mm ID (semicapillary) have a loading capacity similar to that of packed columns, which they replace in many analyzes, with better resolution, higher chemical inertness and lower analysis time. The 0.32-0-53 mm ID columns can be used with either the injector for capillary columns or with the packed column injector, due to the high ow-rates at which they can operate. In the increasingly used GC-MS systems it is recommended to work with small ID columns (0.10mm, 0.15mm, 0.18mm, 0.20 mm and 0.22 mm) so as not to exceed the capacity of the vacuum system. Recently, capillary columns of 0.1 mm ID have appeared on the market. These generate high plate numbers or, in other words, to reduce analysis time without losing resolution. The high efficiency of these columns (7000-10000 plates/meter) allows the resolution of complex samples using shorter column lengths, thus with very short analysis times, with the resulting cost reduction for the laboratory. Evidently, their loading capacity is a limiting factor and in order to obtain the best performance from these columns we need to take into account instrumental factors (injector-detector). Film Thickness The lm thickness of the stationary phase deposited inside the capillary column exerts an inuence on the number of effective theoretical plates that can be obtained with the column for a given separation, on its loading capacity, on the bleed level and on the elution temperature of a compound. A lm thickness of 0.25-0.32 mm is the standard thickness allowing for a compromise between loading capacity and resolution; and for the injection of samples with a wide volatility range. Thick lms increase retention of the most volatile components whereas thin lms provide faster elution at lower temperatures. As a general rule, thin lms (0.1 µm) must be used for compounds with a high molecular weight such as triglycerides, antioxidants, etc., which have elution temperatures over 300°C. Thick lms must be used for low boiling substances because thick lms increase the interaction between the substances and the stationary phase. Specically, 3-5 µm lms are used to separate solvents, gases, and very volatile substances at room temperature or lower. When the thickness of the stationary phase increases, thermal stability decreases, and thus the bleed level is higher which will limit the maximum operating temperature of the column. The ß factor denes the relation between the column internal diameter and the stationary phase thickness, thereby helping you to select the most appropriate column for your analysis. In addition, the ß factor allows for the easy exchange of columns since, for a given analysis with the same stationary phase, similar ß factors will result in the same or very similar retention times and capacity factors. Of course, this implies taking into account the column loading capacity (phase thickness and internal diameter). Cap. 1: 4 Konik-Tech
GC CAPILLARY & PACKED COLUMNS ß Factor ß Column suitable for the separation of: >400 High molecular weight compounds 100 - 400 All purpose use
Page 1 and 2: THE KONIK COMMITMENT The KONIK Grou
Page 3: Contents: 1 · GC Capillary & Packe
Page 6 and 7: GC CAPILLARY & PACKED COLUMNS KONIK
Page 10 and 11: GC CAPILLARY & PACKED COLUMNS LIST
Page 12 and 13: GC CAPILLARY & PACKED COLUMNS KONIK
Page 14 and 15: GC CAPILLARY & PACKED COLUMNS 0.25
Page 20 and 21: GC CAPILLARY & PACKED COLUMNS CAPIL
Page 22 and 23: GC CAPILLARY & PACKED COLUMNS Split
Page 24 and 25: GC CAPILLARY & PACKED COLUMNS Valco
Page 26 and 27: GC CAPILLARY & PACKED COLUMNS FERRU
Page 28 and 29: GC CAPILLARY & PACKED COLUMNS Glass
Page 30 and 31: GC CAPILLARY & PACKED COLUMNS SOLID
Page 32 and 33: GC CAPILLARY & PACKED COLUMNS PACKI
Page 35 and 36: 2 · Ferrules, Fittings & Konik Ins
Page 37 and 38: FERRULES, FITTINGS & KONIK INSTRUME
Page 49: FERRULES, FITTINGS & KONIK INSTRUME
Page 52 and 53: GC SEPTA PREMIUM SEPTA Premium Sept
Page 54 and 55: GC SEPTA MarathonTM Septa Excellent
Page 56 and 57: GC SEPTA Microsep ® Septa F-138 Th
Page 59 and 60:
4 · Syringes
Page 61 and 62:
SYRINGES HAMILTON DIGITAL SYRINGES
Page 63 and 64:
SYRINGES 900 Series - The economica
Page 65 and 66:
SYRINGES 1000 Series - The high per
Page 67 and 68:
SYRINGES 0.5 µl Syringe with Stain
Page 69:
SYRINGES ACCESSORIES Needle Cleanin
Page 72 and 73:
GAS HANDLING & PURIFICATION KONIK D
Page 74 and 75:
GAS HANDLING & PURIFICATION Snoop
Page 76 and 77:
GAS HANDLING & PURIFICATION REGULAT
Page 78 and 79:
GAS HANDLING & PURIFICATION Gas Sam
Page 80 and 81:
GAS HANDLING & PURIFICATION GAS PUR
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GAS HANDLING & PURIFICATION New ZPu
Page 84 and 85:
GAS HANDLING & PURIFICATION All-In-
Page 86 and 87:
GAS HANDLING & PURIFICATION Moistur
Page 88 and 89:
GAS HANDLING & PURIFICATION Split V
Page 90 and 91:
GAS HANDLING & PURIFICATION TUBING
Page 93 and 94:
6 · Gas Generators
Page 95 and 96:
GAS GENERATORS Nitrogen Generators
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GAS GENERATORS LCMS NITROGEN GENERA
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HPLC COLUMNS KONIK HPLC COLUMNS Ana
Page 102 and 103:
HPLC COLUMNS 1· KONIK KWIK-SNAP HP
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HPLC COLUMNS HPLC COLUMN-CARTRIDGES
Page 106 and 107:
HPLC COLUMNS KWIK-SNAP CARTRIDGE NU
Page 108 and 109:
HPLC COLUMNS 2· KONIK MEDITERRANEA
Page 110 and 111:
HPLC COLUMNS MICROBORE COLUMNS 0.21
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HPLC COLUMNS EUROPA COLUMNS ANALYTI
Page 114 and 115:
HPLC COLUMNS ULTRARAPID COLUMNS 0.4
Page 116 and 117:
HPLC COLUMNS 5· KONIK EXTRASIL Col
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HPLC COLUMNS ULTRARAPID COLUMNS 0.4
Page 120 and 121:
HPLC COLUMNS 6· KONIK ADVANTIX ODS
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HPLC COLUMNS 8· KONIK NUCLEOSIL Co
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HPLC COLUMNS MICROBORE COLUMNS - NU
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HPLC COLUMNS 9· KONIK LICHROSORB C
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HPLC COLUMNS 11· KONIK SUPERSPHER
Page 130 and 131:
HPLC COLUMNS GUARD COLUMNS AND FILT
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8 · HPLC Fittings & Accessories
Page 135 and 136:
HPLC FITTINGS & ACCESSORIES Membran
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HPLC FITTINGS / ACCESSORIES Valco N
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HPLC FITTINGS & ACCESSORIES TUBING
Page 141 and 142:
HPLC FITTINGS & ACCESSORIES Binary
Page 143 and 144:
HPLC FITTINGS & ACCESSORIES HPLC SO
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HPLC FITTINGS & ACCESSORIES Descrip
Page 148 and 149:
GC/HPLC/MS MAINTENANCE KONIK INSTRU
Page 150 and 151:
GC/HPLC/MS MAINTENANCE FPD Detector
Page 152 and 153:
GC/HPLC/MS MAINTENANCE SOURCES CLEA
Page 154 and 155:
GC/HPLC/MS MAINTENANCE GENERAL SUPP
Page 156 and 157:
GC/HPLC/MS MAINTENANCE Mirror on a
Page 159 and 160:
10 · Sample Preparation
Page 161 and 162:
SAMPLE PREPARATION General Extracti
Page 163 and 164:
SAMPLE PREPARATION Normal Phase SPE
Page 165 and 166:
MININERT ® VALVES & REACTION VIALS
Page 167 and 168:
SAMPLE CONCENTRATION, EVAPORATORS A
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SAMPLE PREPARATION Hamilton Pipette
Page 172 and 173:
VIALS RECOMMENDED KONIK AUTOSAMPLER
Page 174 and 175:
VIALS AUTOSAMPLER MANUFACTURER / VI
Page 176 and 177:
VIALS CRIMP VIALS 6 - 7 mm Crimp Vi
Page 178 and 179:
VIALS 6 - 8 mm Crimp Vials, Sci-Vi
Page 180 and 181:
VIALS AUC123251 AUC123251 AUC303121
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VIALS 12 x 32 mm Crimp, Wide Mouth
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VIALS NEW ELECTRONIC CRIMPERS & DEC
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VIALS AUC123205 AUC123215 12 x 32 m
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VIALS 12 x 32 mm Screw, Standard Mo
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VIALS 12 x 32 mm Screw, Wide Mouth
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VIALS Miscellaneous 12 x 32 mm Vial
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VIALS AUC154505 AUC154515 15 x 45 m
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VIALS 15 x 45 mm Vials Shell Vials
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VIALS HEADSPACE VIALS - CRIMP TOP R
Page 200 and 201:
VIALS 20 mm Pre-Stuffed Caps and Se
Page 202 and 203:
VIALS AUC237508 AUC237509 HEADSPACE
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VIALS Preassembled Vials • Vial i
Page 206 and 207:
VIALS AUC308001 CRIMP CAPS AND SEAL
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VIALS ACCESSORIES Autosampler Vial
Page 211 and 212:
12 · Standards & Reagents
Page 213 and 214:
STANDARD & REAGENTS MS System Test
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STANDARD & REAGENTS Mechanism Descr
Page 218 and 219:
VACUUM, UHV & SYSTEMS VACUUM PUMPS
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VACUUM, UHV & SYSTEMS NW Blank Flan
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Item Number Index Product Index
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ITEM NUMBER INDEX ITEM Nº PAGE ITE
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Page 247 and 248:
PRODUCT INDEX Syringes Hamilton Dig
Page 249 and 250:
PRODUCT INDEX Packing Accessories G
Page 251:
Notes:
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