CYCLERtest catalog.pdf - BioFrontier Technology

TOOLS FOR VALIDATION OF (q)PCR THERMAL CYCLERS AND INCUBATORS 

DRIFTCON ® 

MTAS ® 

DRIFTCON ® RF

Introduction 

Cycler Validation Equipment…How it started 

It was that unique meeting way back in 1994, when our management team decided to compose a new project-team, that had as goal to develop 

an instrument for monitoring temperature performances of thermal cyclers. At that time it was clear that we had to start from scratch since there 

was no industrial reference available. The basis for this development choice was founded on the awareness of two major issues as being: first the 

fact that there were a number problems occurring in PCR without proper explanation and, secondly the knowledge that PCR would rapidly become 

a diagnostic tool. 

These basic thoughts and the ongoing development over the last 12 year has resulted in the most sophisticated thermal cycler validation tools 

currently available. The tools are widely used in the global marketplace. Surprisingly enough the availability and the intensive use of these tools 

enabled the solid, stable, and ongoing incorporation of PCR and qPCR techniques into the diagnostic market place. 

It goes without saying that we are permanently challenged to improve our systems to the highest possible technological standards. 

Why? We feel we do obligate it to the scientific and diagnostic world. 

Tom Hendrikx 

CEO President 

We present…. 

In this brochure we present systems and services for validating, calibrating and certifying thermal cyclers. Beside the theoretical issues of validation 

we also present, for illustration and educational reasons, real global market data of variables in (q)PCR equipment as well as population variation 

data of (q)PCR cyclers of one brand and type. All of our instruments and its outcomes do fully cover any demanded requirements, and do comply 

with regulations set by ISO, GLP, GMP, FDA, ILAC, ASHI, CAP, EFI, NY-State, Bell-test and other norms and regulations. 

The available systems are: 

MTAS ® 

Validation, Calibration and Certification Service (available as service). 

DRIFTCON ® , a personal temperature drift monitoring system for thermal cyclers and instruments. DRIFTCON ® is, next to the MTAS ® certification, 

the ideal tool to serve as a periodic and continuous validation instrument for your PCR tests. It also offers a convenient and easy means to check if 

your test is still working within its predetermined parameters. 

DRIFTCON ® 

RF, a personal wireless drift monitoring system for thermal cyclers and instruments. DRIFTCON ® RF is typically used in cyclers which 

do not allow access to the reaction block by a regular DRIFTCON ® unit. 

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Website: www.driftcon.com • E-mail: info@driftcon.com 

Website: www.cyclertest.com • E-mail: info@cyclertest.com

Contents 

Contents 

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 

1. A tool that makes your (q) PCR transparent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 

2. Why thermal cyclers are not always accurate & uniform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 

3. Differences between PCR thermal cyclers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 

4. What is measured? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 

5. Cycler Performance: the global picture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 

6. Regulations and Requirements for Validation and Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 

7. Why different products were developed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 

8. Validation and Calibration Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 

9. End-user systems DRIFTCON ® and DRIFTCON ® RF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 

10. The DRIFTCON ® (RF) software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 

11. User-defined DRIFTCON ® applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 


Website: www.cyclertest.com • E-mail: info@cyclertest.com 

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1. A tool that makes your (q)PCR transparent 

Quality control, validation, calibration and certification have become important issues in the contemporary laboratory. 

For some disciplines, a good standardization of the employed procedures has always been obvious, but for others it was historically not that 

evident. In the earlier days molecular biology had always been an area where the methodologies were highly preparative and recipe-wise, 

with less awareness for quality control aspects. 

Nowadays, driven by the amount of diagnostic applications, as well as the fact that investors like to see results supported by hard evidence, 

the consciousness that a good validation and certification is a necessity, has become general practice. 

PCR (Polymerase Chain Reaction) and qPCR are the key reactions in the field of molecular biology. Whether these techniques are applied for 

research, or for the numerous diagnostic purposes (where decisions must be made based on the outcome of the reaction), (q)PCR is leading the 

way. Nevertheless, it remains unclear what contribution the major variables like thermal cyclers, reagents and disposables have on the final results. 

Especially, the effect of temperature performance of a thermal cycler has turned out to be substantial. There are many different brands and types of 

instruments available, and the market is still expanding. As the thermal performance of the many instruments differs to a great extend, a good 

quality control procedure becomes an absolute necessity. 

Already many years ago CYCLERtest started out with its research into (q)PCR-related temperature measurements, and the influence of temperature 

deviations on the results of the (q)PCR reaction. In 1998 the MTAS ® service was introduced in the Benelux market as a means for validation and 

certification of the temperature performance of thermal cyclers. In the beginning of 2002 the system was presented globally and in 2003 the first 

DRIFTCON ® end-user tools were added to complete the range. 

Today, CYCLERtest is proud to call itself world leader in the field of "temperature performance testing". It is conspicuous that the expertise gained 

with the MTAS ® 

service and DRIFTCON ® tools has directed several international developments concerning accreditation and certification of 

associations and laboratories. 

Independency Statement 

CYCLERtest and GENO-tronics, the original developers and designers, are independent and neutral companies. The hardware and software products are verified, accepted and approved by the highest global authorities. 

Both companies are obligated to improve and develop, even better and sophisticated, systems to serve the still growing and demanding market place. Although CYCLERtest and GENO-tronics supply a high number of 

“manufacturers and players” in the marketplace. They refrain from judging or categorizing (q)PCR equipment, which may directly influence the cycler manufacturers market position. However, the market place, users as well 

as cycler manufacturers, will definitively benefit using the outcome and the results of our systems, which leads to improvements in the performance of (q)PCR cyclers to the highest possible levels. By doing so, new sensitive 

(diagnostic), and solid tools and applications can be introduced in the market. 

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2. Why thermal cyclers are not always accurate & uniform 

Influence of the denaturation temperature on enzyme depletion (and reaction yield) caused by 

enzyme heat inactivation. Standard DNA template was used. PCR protocol: annealing 60°C; 60s, 

denaturation 30s. Elongation (extension) at constant time and temperature. 

Thermal cyclers are much more complicated in their design than most users of the instrument can imagine. In general, the design of thermal 

cyclers can be divided into two sections. One section takes care of the programming, thermal control, power regulation, sensor processing etc. 

The other section can be categorized as the block design. The block itself is a construction of several parts: the actual reaction block, the 

temperature sensors, Peltier modules, heaters, sealings, as well as a heat sink etc.. These individual layers are constructed on top of each other and 

they form a "sandwich" construction. 

A diversity of methods are used to balance the sandwich shrinking and expansion, caused by the wide temperature range of operation, 0-100°C 

generally. Although it is beyond the scope of this brochure to discuss every individual construction item, and the impact of these items on the 

performance of a cycler, we will give a brief overview on some of these items and the problems that can occur. Overall one should be more 

amazed about the fact that a cycler is uniform and accurate, rather than that a cycler is always OK unless proven different! 

The actual reaction block 

The block is nothing more than a metal plate (a PCR reaction plate made of metal). The block may have an inconsistent surface and well coating 

and is often not very flat. Because of fluctuations and variations in the production of blocks (CNC milling, drilling and coating), there may be 

different contact angles for tubes, resulting in temperature uniformity and accuracy problems. Since the block has a low mass it is much more 

difficult to balance uniformity. 

Four Peltiers positioned in a cycler, of which 

the reaction block is removed 

Example of a sensor position in a reaction block 

Individual element in a Peltier module 

Some samples of thermistors 

The temperature sensors 

There are different types of sensors, used to control the block temperature of the thermal cycler: e.g. thermocouples, RTD's 

(resistance temperature detectors), thermistors. They all exhibit different inaccuracies and can become defective. 

They could also have bad thermal contact, and be very sensitive to (condensation) liquids. All of these problems may result in temperature 

uniformity and accuracy problems, or even malfunction of the cycler. 

Peltier modules 

Every Peltier module consists of around 50 to 250 individual elements which generate heat or cold. Not every individual module and individual 

element generates the same amount of heat. This can easily lead to so-called "hot" and "cold" spots in Peltier modules. Complete sections of the 

Peltier modules may malfunction, and the limited number of temperature sensors, and so the cyclers, are not capable of recognizing these 

problems. Besides that, difference in height of the Peltier modules and elements may cause bad thermal contact to the block and to the heat sink, 

resulting in block temperature uniformity and accuracy problems. 

Decreased performance in time 

Heaters (if additional incorporated) may not heat uniformly. Furthermore, the thermal contact of a heater to the reaction block is sometimes not 

even. Heaters as well as Peltier modules and sensors are very sensitive to liquid (condensation during cooling). Electrical and mechanical 

components like heaters, Peltier modules, sensors, spring tensions etc. are sensitive to wear. This wear is not always uniform and similar for every 

build-in component. 



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3. Differences between PCR thermal cyclers 

As explained before, there is not a pair of thermal cyclers with the same temperature profile. The accuracy of the block temperature can 

deviate either positively or negatively from the set temperature, and the different wells in a block sometimes show a spread (non-uniformity) in 

temperature up to several degrees °C. The lack of temperature control in some cyclers causes an occurrence of temperature over- and 

undershoots, while trying to reach a plateau. 

A first explanation of these differences might be found in the differences in technology. There are many ways to regulate the temperature in a 

thermal cycler and to bring and hold the block at a certain temperature. Every technology has its pros and cons. The level to which these 

technologies are controlled is essential for the level of performance, but differs greatly. Most cyclers have only a limited level of temperature 

control. Differences between single and multi-sensor control mechanisms are obvious causes for temperature inaccuracies. 

Although a PCR instrument is normally lacking, apart from the lid, any mechanical or moving parts, it does not imply that it cannot be subject to 

wear and tear. The performance of electronic components deteriorate in time, and an intensive use can reinforce this effect. Failure and wear of 

Peltier elements can lead to hot or cold spots in a block. In general, the temperature control mechanisms in thermal cyclers do not recognize the 

loss of performance caused by wear and tear, block expansion and shrinking. It is therefore crucial to monitor the temperature performance in 

time, in order to be aware of these effects. By the same token, a bad thermal performance is often a strong indicator for upcoming failures and 

malfunction of a thermal cycler in the near future. 

Several external factors can also influence the temperature performance of the cycler. Probably, the most important external factor is the way in 

which the tubes are loaded into the block (where to position them?). As a result of the cycler block sensor position (of a certain instrument), a 

specific way of loading the tubes (load-effect) can lead to substantial temperature over-/undershoots, and also block sensor in-accuracies. 

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4. What is measured? 

During the standard validation protocol (30-95-30-90-50-70-60-30 °C) the following parameters are dynamically measured 

- Accuracy 

- Uniformity 

- Ramp Rates 

- Overshoots/undershoots 

- Hold time 

Accuracy 

The most important measured parameters are temperature accuracy and uniformity of a (q)PCR thermal cycler block. The accuracy shows the 

deviation of block temperature from set temperature, while the uniformity is a measure for the spread of temperature between wells of the same 

block. Both parameters depend on the temperature level at which they are determined; the accuracy of a thermal cycler normally decreases at 

elevated temperatures, block non-uniformity becomes more severe. 

In case the temperature accuracy of a thermal cycler is known to be for example -1.0 °C (this implies that the target temperature is not reached), 

one can take this into account while developing and optimizing a protocol. For the parameter of non-uniformity this is much more difficult. 

However, the main advantage of a dynamic and multi-channel measurement is that it is possible to check which wells deviate too much, and avoid 

the use of these hot or cold wells. 

Uniformity 

The cycling speed (or ramp rate) of a PCR thermal cycler is an indication of the slopes in between the temperature holds. Differences in ramp rate 

can influence the course of the reaction. Some cyclers are fast, but less controllable. The material of which the block is manufactured plays an 

important role. Directly related to speed, is the overshoot and undershoot parameter. This is amount the target temperature is exceeded while trying 

to stabilize at a temperature hold. The only way to visualize this is by means of a fast, frequent and dynamic measurement. 

Ramp Rates 

Overshoots/undershoots 

Hold time 



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5. Cycler Performance: the global picture 

Over the last 12 years we have collected tenths of thousands of measurements and validations from a variety of cyclers and brands all over the world. Every temperature measurement, regardless of which type of thermal 

cycling instrument is tested, is performed using a standardized and prescribed temperature protocol. This enables the comparison of the results of every measurement performed and shows if the results of a tested instrument 

deviate from the protocol standards. 

The following picture is extracted from our global data-base and gives a good indication how thermal cyclers, cycler brand and model vary between each other and within their own population. These real data are, most 

likely, the best indicator to support the importance of regular validation of thermal instruments with the correct measurement equipment. 

Statistical Market Values at 95°C 

from a known manufacturer and 

Model (A) 95% population 

boundaries 

Accuracy: 

93.08 °C < A > 95.12 °C 

Uniformity: 

1.04 °C < A > 2.94 °C 

Block Uniformity °C 

Accuracy °C 

Displayed are mean values of multiple measurements of cyclers. X-axis accuracy in degrees centigrade (°C) achieved after 30 seconds at target (95°C) temperature. Y-axis block uniformity (delta 

lowest value-highest value) in degrees centigrade. Bubble size indicates spreads of the population (a larger bubble indicates a higher variation of spread than a smaller bubble). Each dot represents 

a certain brand and type of cycler. Displayed results are extracted from our global database 

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Variation in performance of one type model and brand 

Performance comparison between two models of the same brand 

Performance variation of one type of instrument of 

one brand. Each dot represents a specific instrument 

at a different location in the world. The box 

represents 95% of the population. Mentioned values 

were measured at 95 °C and after 30 seconds 

when reaching the set temperature. X-axis = uniformity, 

Y-axis = accuracy 

Accuracy (°C) 

Performance comparison of two types of instruments 

of one brand. One instrument type: red 

dots, other type: purple dots, both types are from 

same manufacturer. Each dot represents a specific 

instrument at a different location in the world. 

The boxes represent 95% of the population. 

Mentioned values were measured at 95 °C and 

after 30 seconds when reaching the set temperature. 

X-axis = uniformity, Y-axis = accuracy 


Uniformity (°C) 


Performance variation of one type of instrument 

of one brand. Each box represents one set temperature 

(30-50-60-70-90-95) and the variation of 

the population at that temperature after 30 seconds. 

X-axis = uniformity, Y-axis = ∆ accuracy 

(set temperature minus reached temperature). 

The boxes represent 95% of the population. 



Performance increase in time of one type of instrument 

of one brand. Each box represents a time 

frame after reaching set temperature of 95 °C. 

(10-20, 15, 25-35, 30, 85-95, 90 seconds) 

X-axis = uniformity, Y-axis = accuracy boxes 

represent 95% of the population. 





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Application thresholds 

PCR applications work within certain threshold parameters. In any particular case one can think about variables in procedures pre, during and post 

PCR which may influence the generated result. Negative results can include false positives, negatives, no amplification, etc. Some of these 

variables are sample identification, sample extraction, administration, pipetting, reaction vessels, reaction components, cycler performance, 

detection, hybridization, probe and melting curve limits. 

As an example we have used the same reaction conditions as used in an earlier poster publications “The Impact of the Temperature Performance 

of PCR Thermal Cyclers on the Generated Results, and the Obligation for Regular Validation of PCR Thermal Cyclers”. 

Application Thresholds and Cycler Performance 

Influence of denaturation temperature on enzyme depletion (and reaction yield) caused by 

enzyme heat inactivation. Standard DNA template. Running conditions: annealing 60°C, 60 s, 

denaturation 30 seconds at different denaturation temperatures. Cycler block non-uniformities 

will have substantial influence on the generated amplification results. 

Based on the above mentioned gel and specific conditions one can conclude that this application 

protocol only gives trustworthy results if the denaturation temperature of every well is within 

92 and 96 degrees (30 seconds). 

Application thresholds in combination with performance thresholds 

(blue triangle) of a population of Type A and B model 

(same manufacturer) X-axis block uniformity, Y-axis block temperature 

at 95 °C setting and after 30 seconds at plateau. 

A 

B 


Note that only trustworthy results can be generated if the instrument 

performance is within the blue application threshold triangle. In this 

case most of the A cyclers will generate good results and most of the 

B cyclers will not generate trustworthy results if programmed with 

denaturation temperature of 95°C for 30 seconds. 


Type A and type B cyclers are two different models of the same 

manufacturer! 

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6. Regulations & Requirements for Validation & Certification 

Over the last years new laboratory regulations and the necessity for regular validation and calibration are designed by authorities. Depending on 

the type of laboratory, regulation and validation requirements may have been fully or partly integrated into the laboratory structure and SOP’s. If 

focussed on (q)PCR cyclers we have seen a trend that the regulation requirements are nowadays becoming more detailed and moving away from 

general statements as “you have to check your cycler annually” without mentioning how, at which accuracy and at how many well locations. 

Furthermore it becomes completely unclear what to do when a (q)PCR cycler fails at the annual test and which strategy to follow with results 

generated before the validation test. In other words how far do you have to go back and retrospectively re-generate and analyze your results? 

Future regulations will show a trend in increasing the frequency of testing to minimize these issues. DRIFTCON ® meets these kind of requirements 

already. 

General guidelines for validating instruments, as cyclers and incubators can be defined as follows: 

A. Perform the test similar to the original assay and in the working range of the instrument 

B. Test a sufficient and representative numbers of wells 

C. The validation and calibration equipment should be at least 3 times more accurate than the equipment tested 

D. Full trace ability to the highest international standard (ITS-90) 

E. Compliant with FDA 21CFR part 11 

A. Perform the test similar to the original assay and in the working range of the instrument 

This implies that for (q)PCR cyclers a dynamic measurement is the way to go, and that a static measurement is simply insufficient and incorrect. 

B. Test a sufficient and representative numbers of wells 

In other words if one has 96 wells in a reaction block one should at least test on multiple locations (CYCLERtest systems typically use 15 locations). 

Some regulations require a temperature check of all well locations which, if the case, can be accomplished by the use of our "all well" systems. 

In the following columns we have listed some associations which have requirements for (q)PCR cyclers in place: 

CAP (Pathology) 

New York State (Clinical Diagnostics) 

NFSCT (Forensics) 

AAFS 

EFI - ASHI (Immunogenetics) 

FELASA (Animal Health) 

AAVLD (Veterinary) 

GMP 

GLP ISO 17025 

ISO 18159 ISO 20836 

Food Diagnostics 

AACC 

CPA (Pathology) 

Other Quality programs 



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7. Why different products were developed 

The most optimal way of 

“being in control" 

is to combine DRIFTCON ® measurements and 

MTAS ® Certification Services 

It would be a waste of time if the reason why a temperature 

validation was carried out, is only to conform to quality norms and 

requirements. The majority of users have experienced unexplainable 

problems with their (q)PCR experiment, not related to reaction mix 

components (and variables). Fact is that there is often an undefined 

preference for a specific instrument on which "my reaction always 

works". From our experience, this really seems to be the case; not a 

pair of thermal cyclers, even identical types of the same brand, 

shows the same temperature profile. And it is exactly this profile that 

is crucial for the success of the reaction. 

CYCLERtest and GENO-tronics have developed a number of products depending on the requirements of the laboratory. The MTAS ® and Driftcon ® 

systems differ not only in software, hardware, accuracy and ease of handling but, also in the way they are used. 

MTAS ® 

The MTAS ® 

(Mobile Temperature Acquisition System) is used for temperature validation, calibration, service and certification. Accurate handling 

and use of the system requires a qualified CYCLERtest training. 

DRIFTCON ® and DRIFTCON ® RF 

The DRIFTCON ® and DRIFTCON ® RF are end-user tools. The setup of these highly accurate systems is such that they can be easily used in a 

routine laboratory. DRIFTCON ® 

stands for Drift and Control, so control your temperature drift in time. DRIFTCON ® was developed with the 

knowledge that the MTAS ® service is typically performed annually. However, any validation service done with MTAS ® is giving a statement on 

performance at that specific time. It does not guarantee any similar performance for the future. So what to do if a cycler does not pass the annual 

MTAS validation? Question yourself: what is and was the result’s reliability of the test from yesterday, last week, last month and when did my cycler 

start to perform badly? For this reason CYCLERtest developed the DRIFTCON ® system. It simply enables users to check the temperature 

performance frequently and even, in a number of cases, prior starting (q)PCR reactions. So you are not restricted to an annual test and 

DRIFTCON ® 

allows you to verify your thermal cycler performance against the global thermal cycler performance database of the same model and 

type of thermal cycler. 

Fact in many labs, “I have my favorite (q)PCR cycler since it gives me 

nice and good results” 

CYCLERtest products make transparent why 

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8. Validation and Calibration Service 

CYCLERtest services 

The CYCLERtest validation and calibration are either carried out on-site, or at the authorized distributor location using the MTAS ® system. 

The MTAS ® system, is calibrated against the ITS-90 standard, NIST traceable and optionally ISO17025 calibrated. The MTAS ® validation and 

calibration service are accepted by accreditation authorities as a tool for supporting thermal cycler performance. Test results are analyzed and 

reported through an automated process, prohibiting any alterations of the acquired sample data. CYCLERtest 's policy is that only trained and 

certified technicians are allowed to perform validations and certifications with the MTAS ® system. 

CYCLERtest reports 

CYCLERtest results are presented in a detailed report that is generated automatically. CYCLERtest offers different report formats that differ from 

each other with respect to the information presented. Reports range from a compact version (10 pages), a standard version (17 pages) up to an 

extended version (23 pages) per cycler block. Conclusions about the test results as well as recommendations for further use of the instrument are 

included. Temperature data and layouts are encrypted and cannot be modified. The CYCLERtest service complies with the requirements of GLP, 

GMP and other Quality Programs. 

Certificate of Thermal Validation 

A "Certificate of Thermal Validation" accompanies every test report. This certificate represents the results of the measured and calculated main 

parameters of the tested instrument. Notice that there is no "judgement" made whether the instrument performance is superior or inferior; this is left 

to the end-users' interpretation and has always to be combined with the temperature robustness of the used applications. 



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9. End-user systems DRIFTCON ® and DRIFTCON ® RF 

End-user systems DRIFTCON ® and DRIFTCON ® RF 

The DRIFTCON ® 

and DRIFTCON ® RF systems are personal measurement systems. While the MTAS ® measurement system is intended to validate, 

calibrate and certify the thermal performance of a thermal cycler (e.g. monthly) DRIFTCON ® systems provide the opportunity to monitor the 

described thermal parameters in time. The major difference between DRIFTCON ® and DRIFTCON ® RF (wireless) is that DRIFTCON ® is typically 

used in the majority of cyclers and that DRIFTCON ® RF (wireless) is used in those cyclers, which do not allow access to the cycler block while 

cycling, so called closed systems. The RF in DRIFTCON ® RF stands for Radio Frequency and the RF systems are in general more fragile compared 

to the regular DRIFTCON ® systems. 

Pass or Fail is determined by thresholds. Thresholds are supplied by CYCLERtest and determined from market specs and these thresholds can also 

be adjusted in the software to the required values (see also the DRIFTCON ® 

software, thresholds). 

DRIFTCON ® probe fixture 

DRIFTCON ® 

The DRIFTCON ® 

system consists of a so-called "probe fixture", a construction in which the thermistors are positioned in a holder, and placed in the 

thermal reaction block, prior to the measurement. The DRIFTCON ® box is the hardware module that process the collected sensor readings, after 

which the DRIFTCON ® software displays them. Usage of the system is monitored by means of software and the DRIFTCON ® smart card hardware. 

DRIFTCON ® RF 

The DRIFTCON ® RF system consists of a so-called "probe fixture RF", a compact construction in which the thermistors are positioned in a holder, 

with the dimensions of a PCR plate. The fixture also contains all processing electronics, calibration values, power cells, as well as RF 

communication electronics. The DRIFTCON ® RF systems can be used by any closed (q)PCR system (see compatibility chart page 26). While 

cycling, all temperature values are transmitted to the supplied RF USB dongle connected to the computer. Usage of the system is monitored by 

means of software and the DRIFTCON ® RF intelligent hardware. 

DRIFTCON ® RF probe fixture 

DRIFTCON ® and DRIFTCON ® RF test results are analyzed and reported through an automated process, thereby minimizing user intervention and 

prohibiting any alteration of the acquired sample data. 

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DRIFTCON ® monitoring parameters in time 

Every temperature measurement, regardless of which type of thermal cycling instrument is tested, is performed preferably using a standardized and 

prescribed temperature protocol. This enables the comparison of the results of every measurement performed, and shows if the results of the tested 

instrument deviate from the protocol standards. This standard protocol is designed to support all relevant temperatures related to instrument 

specifications and the (q)PCR processes. Data and results of the measurements are collected in a central database at CYCLERtest and the 

instrument specific market performance is calculated. The market performance can differ from the cycler manufacturer specifications. DRIFTCON ® 

users can also participate in a global database network generated by CYCLERtest. Particularly, users can determine how their instrument behaves 

in terms of accuracy, uniformity as well as temperature drift-in time in comparison to the other users of the same cycler type and brand (globally), 

who also participate in the database. 

DRIFTCON ® hardware module 

DRIFTCON ® System Components 

Available in the following configurations: 

* DRIFTCON ® hardware module + Connection cable 

* Intelligent probe fixture 

* Probe fixture storage box 

* DRIFTCON ® SMART-card 

* DRIFTCON ® software 

* Instrument specification set (market specs) 

* Membership of the CYCLERtest global threshold 

specification center 

* DRIFTCON ® system storage case 

* Optional: 

- cycler software specification sets for 

other types/brands of thermal cyclers 

- notebook with DRIFTCON ® software pre-installed 

o 96V-15: 

o 16-6: 

o 48V-12R: 

o 384-15: 

o 16-24: 

o LightCycler ® : 

o SmartCycler TM : 

o Flex 0.2 (flex system): 

o Flex 0.5 (flex system): 

o Flex 384 (flex system): 

15 sensors for 96 x 0.2 ml block formats 

16 sensors for ALL WELL measurements (NY-State) in 96 x 0.2 ml block formats 

12 sensors for 48 x 0.2ml block formats 

15 sensors for 384 block formats 

16 sensors for ALL WELL measurements (NY-State) in 384 block formats 

16 sensors in capillaries, positioned in LightCycler ® Rotor fixture 

16 sensors in SmartCycler TM Cuvettes 

16 flexible sensors for 0.2 ml tube shape blocks 

16 flexible sensors for 0.5 ml tube shape blocks 

16 flexible sensors for 384 wells tube shape blocks 

DRIFTCON ® ALL WELL probe fixture 

DRIFTCON ® individual probe sensor 

DRIFTCON ® SmartCycler TM probe fixture 

DRIFTCON ® LightCycler ® probe fixture 



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DRIFTCON RF ® System Components 

* DRIFTCON ® RF intelligent fixture, electronics and wireless communicator incorporated in the fixture, include also heated lid measurement sensors 

* DRIFTCON ® RF USB dongle, and extension cable 

* DRIFTCON ® RF software 

* Instrument specification set (market specs) 

* Membership of the CYCLERtest global threshold specification center 

* DRIFTCON ® system storage case 

* Optional: 

- cycler software specification sets for other types/brands of thermal cyclers 

- notebook with DRIFTCON ® RF software pre-installed 

Available in the following configurations: 

o 96V-15: 

o 16-6: 

o 96V-15LP: 

o 16-6LP: 

o 384-15: 

15 sensors, for 96 x 0.2 ml block format 

16 sensors, for ALL WELL measurements (NY-State) in 96 x 0.2 ml block format 

15 sensors, for low profile 96 x 0.2ml block format 

16 sensors , for ALL WELL measurements (NY-State) in low profile 96 x 0.2 ml block format 

15 sensors for 384 block formats 

16 



10. The DRIFTCON ® (RF) software 

After start up of the DRIFTCON ® software a pop-up window shows the thermal cyclers to be measured. The cyclers whose turn it is to be validated 

are displayed in red. After selection of an instrument the user proceeds to the main screen, where one can choose from different options, or start a 

DRIFTCON ® measurement sequence. 

Instrument management, (q)PCR Cyclers and Incubators 

The properties of the thermal cyclers and incubators that need to be checked regularly in your lab, can be added or edited, and the measurement 

frequency (daily, weekly etc.) can be defined from this menu. 

Measurement history 

The results of every measurement performed are stored, and can be reviewed easily. Relevant basic information concerning the thermal cycler, 

DRIFTCON ® , user, protocol and external parameters can be displayed, as well as a detailed overview of all the measured parameters: accuracy, 

spread, ramp rates, maximum over/undershoot, average over/undershoot and hold (plateau) time. 

The two most important parameters, temperature spread and accuracy are presented at six different time points or time intervals (10-20s, 15s, 

25-35s, 30s, 85-95s, 90s). At every level the measured and processed values are compared to the threshold values, judged right or wrong 

(within or outside the threshold range ). 

Graphical views 

The temperature uniformity and accuracy can also be presented in a time-graph. With this graph one can visually follow (the development of 

performance) of an instrument in time, between set limits (thresholds). By moving the cursor over the graph, exact values are displayed. A more 

advanced option in drift overview can also be displayed. The advanced option enables overviews and comparisons of brands, models, block types, 

block layouts, temperatures, time sets, for all variables measured as accuracy, uniformity, hold time, ramp rates, over/undershoots, drift and 

selected specification sets. 



17


“Movie” player 

Any temperature acquisition can be replayed using the “movie” player. The “movie” player can be opened for two different acquisitions which 

allow temperature and cycler comparison at any temperature and required channels to be monitored at any time in the cycling process. 

The “movie” player is presented in a way that one has a clear view of the block and the temperature of the block, including the hot and cold 

spots are indicated in color. Furthermore all relevant parameters are dynamically displayed. 

Replay mode for detecting optimal control position 

The “movie” player is an ideal tool to help to optimize your control position basically drilled down to the performance of that individual (q)PCR 

cycler. 

Reporting capabilities 

A nine page report of each measurement can automatically be generated. In these reports all relevant data as well as individual channel 

values are listed. Different report layouts also can be selected. Next to that, a wide variety of drift reports can be generated from either one 

instrument, one type of instrument or all types of instruments, one type of block or all type of blocks, with a free selection of any of the 

variables as accuracy, at which time, uniformity, heat rate, cool rate, plateau, drift, selected specification sets etc. 

18 




Pass or fail criteria 

Pass or fail is depending on the selected thresholds. There are two options when it comes to thresholds: (1) choosing to compare to the 

market specifications (the average worldwide performance of a certain brand and type of cycler), or better, (2) empirically determine the 

limits of a certain test (e.g. a HIV detection), and set the found values as pre-determined thresholds. The pro-active dynamic threshold 

database is accessible and automatically loaded by using the synchronize button. In other words, the software features fully automated 

synchronization functionality of market specs. 

Instrument-based thresholds (market specs) 

Instrument thresholds (the average market performance for one specific instrument and block type) are provided with the purchase of any 

DRIFTCON ® and DRIFTCON ® RF system. Other brand/block-type specific threshold settings may be purchased as software "add-on" 

(optional). These specific instrument thresholds are extracted from the global CYCLERtest database, which contains numerous measurements 

of machines of that same brand and type. These values are provided as a mean plus or minus 1.96 x the standard deviation representing 

95% of the population. 

Application-based thresholds 

Although the design of the application thresholds is much more difficult and time-consuming, application thresholds are superior to 

instrument thresholds. In other words: application thresholds offer a real view whether or not the used cycler is suitable for this application. 

In case of a commercial (diagnostic) kit (PCR, LCR, qPCR, RT-PCR etc) one may request the temperature profile thresholds from the kit 

manufacturer. Should the kit manufacturer not have these thresholds available, then it's worth visiting or participating in our discussion forum 

on the DRIFTCON ® website: www.driftcon.com 

User management 

Three user levels can be defined: Administrator, Controller, and User. The Administrator has full access to all possibilities of the software. 

This person can create other users and change their settings, set threshold values and alter other controls. The Controller is not able to do 

this, but can add and edit instruments. A User is just able to perform measurements and view results only. 

Synchronization of results 

To be able to judge the DRIFTCON ® results versus a frame of reference, it is possible to exchange measurement data with the CYCLERtest 

database. In exchange of these values access is granted to the most up-to-date market specifications of the brand and type of instrument 

used. 



19

11. User-defined DRIFTCON ® applications 

Typical users of the DRIFTCON ® and DRIFTCON ® RF systems 

1. Quality managers of laboratories 4. PCR end users (research) 7. Inspectors, auditors and other regulatory officials 

2. PCR kit manufacturers 5. Thermal cycler manufacturers 8. Purchase officers 

3. PCR end users (diagnostics) 6. Technical service departments 9. Users of a Real-Time PCR thermal cycler 

1. Quality Manager Laboratory 

The quality manager is responsible for all the standardization and quality issues in the laboratory. DRIFTCON ® enables the quality manager to 

design the regulatory requirements for thermal cyclers and applications used. Typically, the quality manager has access to DRIFTCON ® at the 

administrator level. DRIFTCON ® allows the quality manager to have full access to the user management system, overall system settings and device 

management. Besides that the quality manager has full access to the trace ability and report options in the software. 

The quality manager has the possibility to set instrument and application thresholds into detail. These settings may then be used to determine if 

the tested instrument is performing within specifications. The quality manager also decides on the test frequency of the PCR equipment, and sets 

clear definitions whether the threshold preferences are based on application(s) or on instruments. 

2. PCR Kit Manufacturers 

These manufacturers provide PCR kits to different markets. Most of these kits are designed to work on one or a few brands and types of cyclers. 

In general, manufacturers recommend that their kit is used in combination with the cycler brand on which it was developed. Typically, when kit 

manufacturers get claims from customers that their kit is not always working well, they cannot reproduce the customer’s findings in their QC lab, 

due to the difference in cycler temperature performance. Kit manufacturers may opt or require to measure customers thermal (q)PCR cycler to rule 

out possible cycler related issues from the kit. 

The use of DRIFTCON ® in developing, improving and marketing PCR kits 

PCR kit manufacturers can use DRIFTCON ® 

to determine the optimum temperature profile of their PCR kit. With DRIFTCON ® one can set 

thresholds for temperature accuracy and block non-uniformity (spread) within which a kit functions. In other words: determining the maximum 

temperature in-accuracy and spread still allowing the generation of a reliable result. Kit manufacturers may consider separating accuracy and 

spread settings. Different protocols for the same kit may be advised depending on the dynamic temperature performance (DRIFTCON ® 

driven) 

of the customer’s thermal cycler. 

Users of commercial kits can request their kit supplier to provide prescribed threshold sets for temperature accuracy and required block uniformity 

of the kit and/or cycler. 

20 




3. PCR End-users (diagnostics) 

This type of end-user generates diagnostic results and supports these results with appropriate evidence like biological controls, and assurance of 

full trace ability and quality performance of the used equipment. Depending on the internal validation schedule, the type of investigations, and the 

impact and importance of the generated result, they should typically use DRIFTCON ® 

on a daily, weekly or monthly base, and trace the drift of 

temperature performance of the cycler in time. The kit manufacturer may strongly recommend the use of DRIFTCON ® . With DRIFTCON ® a decision 

can be made whether a cycler is suited to be used for a certain application. 

Determine your control positions on the cycler 

To generate a trustworthy result every run contains several known controls. Extra security and support of your results can be achieved by determing 

the hottest and coldest area in your cyclers block. The areas can be easily extracted from the data file using the “movie” player of the DRIFTCON ® 

system. 

Non-working kits; kit claims 

Your kit manufacturer may recommend the regular use of a DRIFTCON ® system to exclude variations in results, generated by a lack of 

performance of the used cycler. A kit manufacturer may even require seeing the thermal DRIFTCON ® profile of the used cycler prior to the 

acceptance of kit claims. 

Non-working instruments or “out of specs” instruments 

Instruments may be performing out of “instruments market specs” provided by CYCLERtest. One can request the latest specifications by 

synchronizing to the general CYCLERtest database. Be aware that pass and fail are only indicators as far as one uses the “market specs” as 

thresholds. Optimal “pass and fail” conditions can be achieved by determination of the specific “application thresholds”. 

Temperature threshold settings for a kit (application thresholds) 

Users of commercial PCR kits may request kit manufacturers to provide prescribed threshold set(s) for temperature accuracy and block nonuniformity 

of the kit and/or cycler. A diagnostic PCR end-user may be requested by governmental auditors and other regulatory bodies to provide 

the temperature threshold settings for the supplied kit as well as the actual validation of these data in time (DRIFTCON ® driven). 

4. PCR End-users (research) 

These users are generating experimental results and support these results with appropriate evidence like biological controls. Assurance of full trace 

ability is not necessarily required, but quality approval of the used equipment is more than welcome. As a result of the differences in the 

experiments, and the experience level of users, they will use DRIFTCON ® 

more as a tool to understand, explain and support differences in the 

generated (q)PCR results, based on the temperature performance of cyclers. Brand to brand differences, as well as differences in performance 

within the same brand can be monitored and explained. They typically use the outcome of the DRIFTCON ® measurements to adjust the 

temperature profiles of different cyclers and ensure the generation of similar results on different machines. They could be using the DRIFTCON ® 

knowledge for future cycler purchases. DRIFTCON ® is typically used on a monthly basis. 



21



Generate a trustworthy result every run contains several known controls. Extra security and support of your results can be achieved by determing 

the hottest and coldest area in your cyclers block. The areas can be easily extracted from the DRIFTCON ® validation file using the movie player of 

the DRIFTCON ® or DRIFTCON ® RF system. 

5. Cycler Manufacturers and Suppliers 

A cycler manufacturer/supplier provides cyclers to the different market place(s). Typically, cycler manufacturers and cycler suppliers get claims from 

end users that their cycler is not always working well, although they cannot reproduce the customer’s findings in their test lab. The use of 

DRIFTCON ® 

as a final factory release test is therefore strongly recommended. A cycler manufacturer may consider recommending the regular use 

of a DRIFTCON ® 

system to end users, to exclude variations in results caused by non cycler related issues (enzymes, primers, disposables, incorrect 

programming etc.). At the same time, a cycler manufacturer may consider requesting the DRIFTCON ® thermal profile of the used cycler, prior to 

acceptance of cycler-performance claims. If you manufacture cyclers for Real-Time applications, the use of DRIFTCON ® or DRIFTCON ® RF is 

strongly recommended. 

Use of DRIFTCON ® in thermal cycler manufacturing 

Cycler manufacturers can use DRIFTCON ® or CYCLERtest technology to determine the temperature profile of their cyclers. It can be used as an 

“in process control” during the manufacturing of the cycling blocks. DRIFTCON ® will recognize minor inaccuracies in the block construction, like 

incorrectly placed sensors, non-uniform Peltiers, (so called “hot” and “cold” spots), block sandwich tension problems, heater non-uniformities, etc. 

By using DRIFTCON ® 

one can set thresholds towards temperature accuracy and block uniformity of a specific cycler type. In other words: define 

what the maximum temperature in-accuracy and block non-uniformity of the cycler is, which can still be acceptable before you categorize it as 

being “out of specs”. Cycler manufacturers may consider upgrading their “instrument specs” with data based on high accuracy, dynamic, multi 

sensor temperature measurements (DRIFTCON ® 

driven). 

Defective instruments or “out of specs” instruments 

Your instrument may be performing out of “instruments specs” provided by CYCLERtest. You can request the latest instrument market specifications 

by synchronizing to our general database. 

22 




6. Technical Services Departments 

Typically, these departments are responsible for the service and maintenance of instruments in their institute or company. In some cases the 

purchase department requests their (technical) advice. They are responsible for handling claims of “not correctly working” or “incorrect” cyclers, 

but normally do not have access to highly accurate, multi-sensor and dynamic measurement systems like DRIFTCON ® . The use of DRIFTCON ® 

as a “diagnostic tool” is highly recommended. Technical services departments should consider using a DRIFTCON ® system on a regular base to 

exclude variations in results generated by a lack of performance of non-cycler related issues (enzymes, primers, protocol, disposables etc.). 

If the institute/company has cyclers for real-time applications, the use of DRIFTCON ® or DRIFTCON ® RF is strongly recommended. 

In a number of cases these departments may consider to qualify for a sub-license of MTAS ® -service systems. 

Positioning the DRIFTCON ® probe fixture in a PCR block 

Use of DRIFTCON ® in service departments 

DRIFTCON ® should be used to determine the temperature profile of the cycler. DRIFTCON ® will recognize minor inaccuracies in the block 

construction, like incorrectly placed sensors, non- uniform Peltiers (so called “hot” and “cold” spots), block sandwich tension problems, heater nonuniformities 

etc. as well as wearing and drift problems in time. By using DRIFTCON ® 

one can set thresholds for temperature accuracy and block 

uniformity of the specific cycler type. In other words: define what the maximum temperature in-accuracy and block non-uniformity of the cycler is, 

which can still be accepted before it is categorized as being “out of specs”. Service departments may consider upgrading their “instrument specs” 

with data based on high accuracy, dynamic, multi sensor temperature measurements (DRIFTCON ® 

driven). 

Incoming quality control 

In institutes/companies, DRIFTCON ® might serve as an incoming quality control tool for newly purchased regular and Real-Time thermal cyclers. 

It is a way to accept or reject instruments, based on the proof provided by DRIFTCON ® measurements. 

7. Inspectors, Authorized Auditors and other Regulatory Officials 

Inspectors, authorized auditors and other regulatory officials are investigating and certifying quality issues in laboratories. They expect hard 

evidence from the audited laboratory, since they have to comply to certain regulations, like GMP, GLP, ISO, FDA and others. DRIFTCON ® has been 

designed in such a way that an inspector can design or even advice in the regulatory requirements for thermal cyclers, and the used PCR 

applications in a lab or department. The inspector should have access to DRIFTCON ® at the administrator level. DRIFTCON ® 

allows the 

administrator full access to the user management system, overall system settings and device management. Besides that, the inspector has full 

access to the trace ability and report options in the software. The software and hardware are compliant with FDA regulations (part 21 CFR part 

11). 

Drift of instruments or applications in time 

Using the report button one can display drift of temperature and uniformity. All tested temperatures and times can be displayed and printed. 

Generally, we recommend targeting the higher temperatures (90 and 95°C), and to different time points and scales. 

Reporting thresholds 

The thresholds displayed are the ones that are used during the actual measurements. Any changes in thresholds are displayed in the report. 



23


8. Purchase Officers 

Purchase officers of (q)PCR thermal cyclers and (q)PCR related kits are responsible for the purchase of medium to high capital equipment. 

They are keen to optimize the balance between quality, price and reliability. And therefore they are always searching for tools that can help in 

finding that balance. Besides using these tools they are communicating and fine-tuning their judgment with the end-users’ department and possibly 

with the technical service department of their institute. 

Cycler demonstrations 

Requesting a DRIFTCON ® temperature profile (temperature performance) from the end-user might be considered, to support the purchase of a 

specific cycler. Depending on the individual situation the technical service department may be asked to advice with the DRIFTCON ® temperature 

profile. Profiles can be requested for all tested cyclers and support the purchase with the provided temperature profiles. 

Incoming Quality Control 

In the institute/company, DRIFTCON ® and DRIFTCON ® RF might serve as an incoming quality control tool, for newly purchased regular and 

Real-Time thermal cyclers. It is a way to accept or reject instruments, based on the results provided by DRIFTCON ® or DRIFTCON ® RF 

measurements, and to advice the accounting department on the payment release for the equipment. 

Purchase of (diagnostics) PCR kits 

Users of commercial PCR kits may request kit manufacturers to provide prescribed threshold set(s) for temperature accuracy and block uniformity of 

the kit and/or cycler. A kit manufacturer may be requested by governmental auditors and other regulatory bodies to provide the temperature 

threshold settings for the supplied kit as well as the actual validation of these data in time (DRIFTCON ® and DRIFTCON ® 

RF driven). 

9. Users of a Real-Time PCR Thermal Cycler 

Users of a Real-Time PCR thermal cycler are generating Real-Time PCR (diagnostic) results and would like to support these results with appropriate 

evidence like biological controls, and assurance of full trace ability and quality of the used equipment. We therefore strongly recommend the use 

of DRIFTCON ® or DRIFTCON ® RF for Real-Time applications. Depending on the internal validation schedule, the type of investigations, and the 

impact and importance of the generated results, one should typically use DRIFTCON ® 

on a daily, weekly or monthly base, and trace the drift of 

temperature performance of a cycler in time. The kit manufacturer may strongly recommend the use of DRIFTCON ® . With DRIFTCON ® one can 

test if the cycler suitable to run your application. 

Where would you put your controls in this qPCR cycler? 


To generate a trustworthy result every run contains several known controls. Extra security and support of your results can be achieved by determing 

the hottest and coldest area in your cyclers block. The areas can be easily extracted from the DRIFTCON ® 

validation file using the “movie” player 

of the DRIFTCON ® or DRIFTCON ® RF system. 

24 




Block non-uniformities 

Every thermal cycler has temperature non-uniformities. Be aware of the fact that you may have individual “hot” and “cold” spots in the reaction 

block. These spots are normally not detected or not early enough with biological controls, or by the use of internal sample controls. In that respect 

it is important to realize that the results of fluorescence signal, yield and melting curves are in direct relation with these temperatures. If, e.g. the 

difference would just be 1°C, the impact on the generated yield and fluorescence signal would already be enormous. 

Non-working kits, kit claims 

The kit manufacturer may recommend the regular use of a DRIFTCON ® system to exclude variations in results, generated by a lack of thermal 

performance of the used cycler. A kit manufacturer may even require studying the thermal DRIFTCON ® 

profile of the used cycler prior to the 

acceptance of kit claims. 

Non-working instruments or “out of specs” instruments 

The instrument may be performing out of “instruments specs” provided by CYCLERtest. One can obtain the latest specifications by synchronizing to 

the general CYCLERtest database. 

Temperature threshold settings for a kit 

Users of commercially available kits may request kit manufacturers to provide prescribed threshold set(s) for temperature accuracy and block 

uniformity of the kit and/or cycler. Governmental auditors may request a kit manufacturer and other regulatory bodies to provide the temperature 

threshold setting for the supplied kit as well as the actual validation of these data in time (DRIFTCON ® driven). 

Melting curve accuracy 

In qPCR procedures fragment identification and size are often determined using a so called “melting” curve. Validation and knowledge of the 

accuracy and uniformity of the block at melting curve temperatures are helpful tools to support the generated results. 

DRIFTCON ® “movie” player 



25


Which cycler models can be measured? 

Basically any (q)PCR cycler and dry-block incubator can be measured. Furthermore non related “block cyclers” 

as LightCycler ® , SmartCycler ® , COBAS ® , TaqMan ® and so called Fast and closed systems can be measured and 

validated. Contact CYCLERtest or an authorized distributor for advice for which type and configuration of 

DRIFTCON ® or DRIFTCON ® RF is most appropriate to measure your instrument range. 

MTAS ® Service 

All (97%) existing brands and types of cyclers including Real-Time cyclers. 

End-user Systems 

Manufacturer Regular Cycler Model System 

Manufacturer Real-Time Cycler Model System 

Techne all types Driftcon ® 

Applied Biosystems 240, 2400, 2700, 2720, 9600, 9700, 9800 Fast Driftcon ® 

Applied Biosystems 5700, 7000, 7700 Driftcon ® 

Applied Biosystems 7300, 7500, 7900HT Driftcon ® RF 

Bioer XP, Genius, Life Express Driftcon ® 

Biometra Uno, Uno II, Tpersonal, T1, Tgradient, T3, Tprofessional, TRobot Driftcon ® 

Bio-Rad iCycler TM , iQ TM 5, my iQ TM Driftcon ® 

Bio-Rad, MJ Research type Opticon TM 1, Opticon TM 2, MiniOpticon TM , Chromo 4 TM Driftcon ® 

Bio-Rad Gene-Cycler ® , iCycler ® , MyCycler ® Driftcon ® 

Bio-Rad, MJ Research type PTC ® -100, 150, 200, 220, 221, 225 Driftcon ® 

Cepheid SmartCycler ® Driftcon ® 

Corbett Research Palm-Cycler TM Driftcon ® 

Eppendorf MasterCycler ® ep, realplex ® Driftcon ® RF 

Eppendorf Mastercycler ® , all types Driftcon ® 

Evogen Evocycler TM Driftcon ® 

Finnzymes all types Driftcon ® 

Roche LightCycler ® 480 Driftcon ® RF 

Roche LightCycler ® 1, LightCycler ® 2, COBAS ® Taqman ® Driftcon ® 

GRI G-storm TM , all types Driftcon ® 

Stratagene Mx3000P ® , Mx3005P ® Driftcon ® (RF) 

MWG all types Driftcon ® 

Techne Quantica ® Driftcon ® 

Takara all types Driftcon ® 

Contact CYCLERtest or a CYCLERtest authorized distibutor for exact system configuration. 

26 




CYCLERtest service (using the MTAS ® system) 

It is offered as a service on-site, as well as in house (at the authorized 

distributor's location). It is recommended every year, or after every 250 

runs (whichever comes first). 

o 

o 

o 

o 

Measurements are performed dynamically, static and dynamic results are reported 

Measurements on virtually all thermal equipment, regardless of brand or type 

Measurements are traceable to the ITS-90 Scale 

Measurements and test systems are NIST traceable 

o System accuracy is better than ±0.1°C over a range of 10 - 100°C 

o Measurement range 10 - 100°C 

o 

o 

o 

o 

o 

o 

o 

Measurements of 16 or more channels simultaneously (2 Hz/channel) 

Test reports in 3 possible configurations + additional custom reporting 

"Certificate of Thermal Validation" for the instruments 

Adjustment of the device if possible and necessary 

Conclusions and recommendations regarding the use of the device 

Cycler to cycler protocol transition service available 

ISO 17025 calibration optional 

DRIFTCON ® end-user product 

Use is recommended daily, weekly or monthly 

o 

o 

o 

Measurements are performed dynamically 

Measurements on virtually all thermal equipment, regardless brand or type 


o Measurements and test systems are NIST traceable 

o System Accuracy is better than ±0.15°C over a range of 10 - 100°C 

o 

o 

o 

o 

o 

o 

o 

Measurement range 10-100°C 

Measurements of up to 16 channels simultaneously (2 Hz/channel) 

Retrospective analysis, and movie player in place 

Test reports of drift, uniformity and accuracy, heat rate, cool rate, hold time 

Access to global DRIFTCON ® database 

Cycler to cycler protocol transition tool 

Conform GMP, GLP, ISO, RvA, governmental and association regulations 

DRIFTCON ® RF end-user product 

Use is recommended daily, weekly or monthly 

o 

o 

o 

o 

Measurements are performed dynamically 

Measurements on all (q) PCR closed systems non accessible by regular 

DRIFTCON systems, regardless brand or type 


Measurements and test systems are NIST traceable 

o System accuracy is better than ±0.20°C over a range of 10 - 100°C 

o 

o 

o 

o 

o 

o 

o 

Measurement range 5-100°C 

Measurements of up to 16 channels simultaneously (2 Hz/channel) 

Retrospective analysis, and movie player in place 

Test reports of drift, uniformity and accuracy, ramp rate, cool rate, hold time 

Access to global DRIFTCON ® database 

Cycler to cycler protocol transition tool 

Conform GMP, GLP, ISO, RvA, governmental and association regulations 

DRIFTCON ® MTAS ® -16 DRIFTCON ® RF MTAS ® -96 

Number of sensors 15 16 16 96 

Cycler Probe fixture yes yes (optional) wireless yes (optional) 

Intelligent Probes yes yes yes yes 

Application for end-user service dept* 50+ / dist. end-user distributor service 

Validation yes yes yes yes 

Certification no yes+ no yes 

Cycler type all all (q) PCR close all 

Purchase option sales lease sales/service lease 

System accuracy ±0.15 ±0.10 ±0.20 ±0.10 

Drift overviews yes no yes optional 

Accuracy yes yes yes yes 

Uniformity yes yes yes yes 

Ramp Rates yes yes yes yes 

Over/Undershoots yes yes yes yes 

Hold time yes yes yes yes 

Heated lid no no optional optional 

“Movie” Player yes no yes yes 

Performance level + +++ ++ ++++ 

Calibration required 1 year 1 year 1 year 1 year 

Report options ++ ++++ ++ ++++ 

ISO 17025 calibration yes / flex set only yes (optional) n/a yes (optional) 

near future near future 

Trademarks: 

CYCLERtest is a trademark of CYCLERtest BV, The Netherlands. 

GENO-tronics, DRIFTCON and DRIFTCON RF, MTAS are registered trademarks of GENO-tonics, BV The Netherlands. 

Evocycler is a trademark of Evogen Ltd. 

G-storm is a trademark of Gene Technologies Ltd. 

iCycler, iQ5, my iQ,PTC-100, PTC-150, PTC-200, PTC-220, PTC-221, PTC-225 are registered trademark of Bio-Rad Laboratories, Inc. 

LightCycler is a registered trademark of the Roche Diagnostics GmbH. 

Mastercycler is a registered trademark of Eppendorf AG. 

Mx3000P, Mx3005P and Stratagene ® are registered trademarks of Stratagene company, USA 

Opticon 1, Opticon 2, MiniOpticon, Chromo 4 are trademarks of Bio-Rad Laboratories, Inc. 

Palm-Cycler is a trademark of Corbett Research, Australia. 

SmartCycler is a trademark of Cepheid Inc. 

Uno, Uno II, Tpersonal, T1, TGradient, T3, TProfessional, TRobot are (registered) trademarks of Biometra Biomedizinische Analytik GmbH. 

Quantica is a registered trademark of Techne Ltd and Techne, Inc. 

GeneAmp is a registered trademark of Roche Molecular Systems Inc. 

COBAS is a trademark of a Member of the Roche Group 

TaqMan is a registered trademark Applied Biosystems. 

The PCR (Polymerase Chain Reaction) Process is covered by patents owned by Hoffman-La Roche, Inc. (4.683.195, 4.683.202, 4.965.188). 

Copyright CYCLERtest 01-2007. All rights reserved. 



27

Your local distributor: 

CYCLERtest BV Phone: +31+(0)45 533 87 33 

Rötscherweg 61, Entrance C Fax: +31+(0)45 533 87 94 

6374 XW Landgraaf Website: www.cyclertest.com - www.driftcon.com 

The Netherlands E-mail: support@cyclertest.com - support@driftcon.com 

BPCTi Inc, Phone: 919 806 8811 

2933 South Miami Blvd. Fax: 919 806 2014 

Suite 121 Website: www.bpcti.com - www.driftcon.com 

Durham, NC 27703, USA E-mail: info@bpcti.com - support@driftcon.com

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